JPH10165120A - Digestive tract function improving and cholesterol elevation suppressing agent originated from barley and health food containing the same agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a digestive tract function improving and cholesterol elevation suppressing agent originated from barley and health food containing the agent manufacturable in a simpler process without using dangerous processing agents or chemicals, by using barley bran fully containing dietary fibers similarly to wheat bran as a raw material. SOLUTION: This suppressing agent contains bran generated at the time of manufacturing edible barley, the barley bran is in the range of a grain cleaning yield 80-100% and it is obtained by grain size sorting of the barley bran by a sieve and turning the grain sized to the range of passing through a 60M sieve (a mesh opening is 0.25mm). Also, the health food is obtained by adding the digestive tract function improving and cholesterol elevation suppressing agent originated from the barley into food for more than 13%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a barley-derived gastrointestinal tract function improving agent, a cholesterol increase inhibitor, and a health food containing the agent derived from barley bran.

[0002]

2. Description of the Related Art Dietary fiber
r) is not generally used in the body, but rather is treated as an obstacle to the efficiency of nutrient utilization, and has been neglected in conventional nutrition. Food refinement has been devised so that brown rice is polished to eat white rice, and fibers are removed as much as possible. However, since the 1970s, it has been proven in various countries that dietary fiber is effective in preventing various diseases, and the importance of ingestion has been recognized.

[0003] In recent years, it has been recognized that dietary fiber obtained from the hulls of cereals, beans and the like has obesity prevention, improvement of bowel movement, and various organizing and activating effects, and has attracted attention as a health food. In particular, wheat bran is an insoluble dietary fiber whose main component is cellulose, hemicellulose, and lignin in the epidermis remaining after the endosperm and germ are separated in the milling process. Its constipation prevention effect has been known for a long time. Recently, cereal foods made mainly from wheat bran have been approved as foods for specified health use, and the effect of "intestinal action" has been granted. There is also strong interest in protective effects against diabetes, cardiovascular disease, colorectal cancer, intestinal diverticulosis, and the like. For example, Japanese Patent Application Laid-Open No. 58-41824 discloses that wheat, bran or starch, protein, lipid,
There has been proposed a serum cholesterol increase inhibitor containing, as a main component, hemicellulose extracted from the remainder from which minerals and the like have been removed with an alkali.

[0004] Japanese Patent Application Laid-Open No. 62-79756 proposes a wheat bran containing low fats and oils, which is obtained by washing and defatting wheat bran.

Further, Japanese Patent Application Laid-Open No. 1-265868 proposes a dietary fiber product obtained by subjecting wheat bran to pulverization and gas classification.

[0006]

However, the invention of JP-A-58-41824 requires complicated operations such as alkali extraction and protein removal in order to increase the content of hemicellulose. This leads to high manufacturing costs,
It could not be easily used as an additive to food and drink.

In addition, since a treating agent or a chemical such as an alkali or a deproteinizing agent, which may cause a danger to the human body, is used, it is necessary to pay close attention so that these do not remain in the product.

Further, the inventions of JP-A-62-79756 and JP-A-1-265868 also disclose a wastewater treatment system for water treatment and an alcohol and recovery facility for degreasing, and a solvent treatment treatment for the latter. Although not done,
It uses a dryer, a shifter, a classifying rotor, etc., and is not a simple processing operation.

Accordingly, the present invention provides a barley which can be produced in a simpler process without the use of dangerous treating agents or chemicals, using barley bran rich in dietary fiber in the same manner as wheat bran. It is an object of the present invention to provide a gastrointestinal tract function improving agent, a cholesterol elevation inhibitor, and a health food containing the same.

That is, the present inventors have conducted intensive studies to achieve the above object. As a result, among the bran fractions having a yield of 80 to 100% generated when milling barley, in particular, a 60M sieve (opening 0.25 mm). The passage section of, compared to commercially available wheat bran material, has been found to have a better gastrointestinal function improving action and cholesterol increase suppression action,
The present invention has been completed.

[0011]

The barley-derived gastrointestinal tract function improving and cholesterol elevation inhibitor of the present invention is characterized by containing bran generated during the production of edible barley.

[0012] The barley-derived gastrointestinal tract function improving and cholesterol elevation inhibitor of the present invention is also characterized in that the barley bran is classified into a milling yield of 80 to 100%.

The barley-derived inhibitor of gastrointestinal tract function improvement and cholesterol elevation inhibitor of the present invention has a dietary fiber content of 4%.
It is also characterized in that the content of hemicellulose is 0% or more and the content of hemicellulose in the total amount of dietary fiber is 60% or more.

The barley-derived gastrointestinal tract function improving and cholesterol elevation inhibitor of the present invention is obtained by sieving the above barley bran with a sieve or the like, and adjusting the particle size to 60M sieve (mesh size 0.25).
mm) It is also characterized as a section of passage.

The health food containing a barley-derived gastrointestinal tract function improving and cholesterol elevation inhibitor according to the present invention is characterized in that the barley-derived gastrointestinal tract function improving and cholesterol elevation inhibitor is added to the food in an amount of 13% or more. And

The milling yield of the raw material of the present invention is 80-
100% barley bran refers to the outer portion of the barley grain, which usually consists of the remainder of the edible barley in the barley milling process. Although the composition of these barley brans differs somewhat depending on the type of barley, for example, barley produced in Canada (quality B1602)
In the case of, the water content is 10.1%, the ash content is 6.1%, and the crude protein is 12.
1%, crude fat 5.3%, and dietary fiber 56.6%. However, the timing of ingestion and the variety are not particularly limited.

In the present invention, the barley bran is first sieved through a 60M sieve to remove a 60M over-segment having a small hemicellulose content in the total dietary fiber content, and a 60M sieve passing segment having a high hemicellulose content (total dietary fiber content is reduced). 40% or more, and the content of hemicellulose in the total amount of dietary fiber is 60% or more). If necessary, further pulverization to adjust the particle size allows the barley-derived inhibitor of gastrointestinal function improvement and cholesterol increase inhibitor of the present invention to be obtained. Incidentally, the amount of dietary fiber in the present invention,
It means an indigestible polysaccharide measured by the Southgate method.

In the present invention, a health food containing a gastrointestinal tract function improving agent and a cholesterol increase inhibitor can be obtained by adding this substance to various foods. Examples of such foods and drinks include breads and confectioneries such as bread, rice crackers, cakes, biscuits, cookies, noodles such as soba, udon, Chinese noodles, and various kinds of dishes such as tempura and fried foods, and dumplings. Is raised.

In this case, the amount of the substance added to the food is preferably at least 13% (by weight) in order to sufficiently impart a gastrointestinal function improving effect and a cholesterol increase suppressing effect.

The substance of the present invention can be used as it is as a health food having a gastrointestinal function improving effect and a cholesterol increase suppressing effect.

According to the present invention, a substance having a gastrointestinal function improving effect and a cholesterol increase suppressing effect can be obtained by a very simple process of separating by a sieve. In addition, since it is not necessary to use an alkali, a deproteinizing agent, and the like, there is no possibility that a harmful treating agent or drug remains on the human body.

In the present invention, among the barley bran fractions having a yield of 80% to 100% when barley is milled, the passing fraction through a 60M sieve is important, and the digestive tract function is clearly improved as compared with commercial wheat bran. And an effect of suppressing cholesterol elevation.

However, there is no significant difference in the category of over 60M sieve compared to commercial wheat bran. The reasons for the effect of improving the digestive tract function are considered to be that the water retention of hemicellulose contained in the passage section through the 60M sieve is extremely high, and that it is easily used by intestinal bacteria.

[0024]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be described below in detail based on examples.

[0025]

EXAMPLE An RM-5C grinding miller and RM-5C were used to feed six-row barley (variety B1602) harvested in Canada in 1995.
The yield was reduced to 80% by continuous milling using a 3C grinding mill (all manufactured by Satake Seisakusho Co., Ltd.). The barley bran component is 30 micro-shifters (Dalton).
It was fractionated into an M sieve (aperture 0.5 mm) over section and a 60 M sieve (aperture 0.25 mm) passage section. Bar the former
It was designated as a ray bran 30 (hereinafter abbreviated as BB-30), and the latter was a Barley bran 60 (hereinafter abbreviated as BB-60). In addition, commercially available wheat bran (hereinafter abbreviated as WB) used in the following test examples was used. All of the above samples were pulverized with a Polytron (manufactured by Hosokawa Micron Corporation) so that all of the samples passed through a 100M sieve (opening: 0.15 mm) in order to eliminate the influence on physiological effects due to differences in particle size. Table 1 shows the results of component analysis of each sample.

[0026]

[Table 1] <Test Example> In order to examine the gastrointestinal tract function improving effect and the cholesterol increase suppressing effect of the sample obtained in Example 1, animal experiments using rats were performed. (A) Experimental feed Table 2 shows the feed composition used in the experiment. The experimental group was a fiber-free (hereinafter abbreviated as FF) group and a cellulose powder (hereinafter abbreviated as CP, manufactured by Toyo Roshi Kaisha, Ltd.).
The BB-30 group, BB-
Each of the 60 groups and the WB group had a dietary fiber level of 5 w / w%.
The experimental feed was included. In addition, BB-30, BB-60,
Each group of WB, based on the general component value, protein, lipid,
The starch levels were adjusted to be equal.

[0027]

[Table 2] (B) Animal breeding method Using 4-week-old Sprague-Dawlay rats (initial weight 70-85 g, manufactured by Tokyo Laboratory Animal Co., Ltd.),
After preliminary breeding for 7 days with a base sample based on the IN-76 composition,
The animals were divided into 5 groups, each group consisting of 7 animals according to body weight, and subjected to the experiment. Rats were placed in stainless steel individual gauges, and the four-zone room was adjusted to a temperature of 23 ± 1 ° C., a humidity of 50 ± 10%, and a 12-hour light / dark cycle (light period: 8:00 to 20:00). Each feed group and water (tap water) shown in Table 2 were allowed to be freely ingested.
This breeding was carried out for a day. (C) Analysis and Statistical Processing Methods The gastrointestinal transit time (hereinafter abbreviated as GTT) was measured twice on the eighth and fifteenth days after the start of the breeding. That is,
The animals were fasted from 8:00 to 20:00 and fed a feed containing carmine as a marker at 0.5 (w / w)%. Every 30 minutes, the presence or absence of the pigment in the feces was confirmed, and the measurement was performed by measuring the time required until the marker was confirmed for the first time (the result is an average of two times).

The amount of feces was measured every day for 5 days before the end of the breeding. After measuring the amount of feces, freeze-dry it,
The amount of dry feces was measured.

In order to examine the effect of each feed group on the characteristics of the digestive tract, the animals were fasted for 3 hours after the end of the breeding period, and the abdomen was opened under Nembutal anesthesia. The cecum was quickly taken out and weighed, then a part of the contents was homogenized with ultrapure water, and the pH was measured with a glass electrode. The remaining contents are frozen in liquid nitrogen and stored at -80 ° C.
The short-chain fatty acids were quantified by a gas chromatograph (GC-12A, manufactured by Shimadzu Corporation) according to the method of Deschner et al.

To examine the effect of each feed group on liver cholesterol level, after laparotomy, the liver was washed with ice-cold physiological saline and mixed with chloroform: methanol (2: 1).
The lipids are extracted according to Folch's method, and total cholesterol is determined by Zak et al., And triglycerin is determined by D.
The method of anno et al., phospholipids was measured by the Bartlett method. To test the difference between the mean values of these analysis values,
Using an AS computer package, numerical values were analyzed by one-way ANOBA, and then a test for significant difference was performed using Duncan's multiple ran.
The test was performed by a get test. Tables 3 to 6 show the results.
Shown in

[0031]

[Table 3]

[0032]

[Table 4]

[0033]

[Table 5] (Below)

[0034]

[Table 6] (D) Description of the results 1) Description of improvement of digestive tract function Tables 3 and 4 show the effects of each test group on the amount of feces, the amount of water in the feces, the intestinal transit time of the contents, and the characteristics of the cecum. Table 5 shows the effect on short-chain fatty acids in the contents. The most characteristic difference was found in the pH of cecal contents.
As compared to the FF group, the CP group and the BB-30 group hardly changed, whereas the cecum contents pH of the BB-60 group significantly decreased. The degree was also significant in comparison with the WB group. The cecal content weight was not significantly different from the WB group and BB-30 group, but was the highest in the BB-60 group (Table 4).
One of these causes is that the difference in component characteristics between BB-60 and the other test groups affects the production of fermentation products (for example, substances that lower the pH such as short-chain fatty acids) ( Table 5). In other words, the BB-60 group contains more substances that are more likely to be decomposed and fermented by the intestinal bacteria as compared with the other test groups, and at the same time, there is a good possibility that the intestinal bacteria are promoted in planting.

Further, considering the balance between the amount of feces and the water content of feces, the BB-60 group had the largest amount of water in feces compared to the other test groups. This indicates that the BB-60 group has high water retention. Also, GTT
Also, BB-60 group compared with FF group, CP group,
Of course, the BB-30 group showed a significant difference as well as being significantly shortened. Although there was no significant difference, the BB-30 group was shorter than the WB group (Table 3). Although the effect of dietary components on GTT is complex, it is generally said that dietary fiber with high water retention increases the amount of feces and thus shortens GTT. Therefore, the BB-60 group most shortened GTT as compared with the other test groups because of the BB-60 group.
It is possible that the water retention of the group itself is sufficiently conventional. In an experiment in which SCFA was directly administered into the colon, it was recognized that butyric acid was the most effective in terms of contraction strength (which is said to have some correlation with GTT). Based on this and this result (Table 5), BB
It can be seen that the characteristics of the composition of short-chain fatty acids which are metabolites of dietary fiber in the -60 group clearly contribute to shortening of GTT.

From the above results, the BB-60 group is clearly superior in improving gastrointestinal function as compared with the other test groups, and the effect is equal to or more than that of the WB group. I understand.

2) Description of suppression of cholesterol elevation Table 6 shows the effect of each test group on liver cholesterol. In the triglycerin amount, the BB-60 test group was significantly lower than the other test groups. Also, the total cholesterol level was lower than the other test groups.
It is said that there is a relatively negative correlation between the amount of short-chain fatty acids produced and the amount of cholesterol. From this, it was considered that the reason why the BB-60 group was superior to WB in suppressing cholesterol elevation was that a large amount of short-chain fatty acids was produced (Table 5).

From the above results, it was found that the BB-60 group had a more excellent cholesterol-inhibiting effect than the WB group.

[0039]

According to the present invention, barley bran, which is rich in dietary fiber like wheat bran, is used as a raw material.
It is possible to provide a gastrointestinal function-improving and cholesterol-inhibiting agent which can be produced in a simpler process without using dangerous treating agents and drugs, and a health food containing the same at very low cost. It became so.

Claims (5)

[Claims] 1. An agent for improving digestive tract function and inhibiting cholesterol elevation derived from barley, characterized by containing bran generated during the production of edible barley. 2. The barley-derived inhibitor for improving gastrointestinal tract function and increasing cholesterol according to claim 1, wherein the barley bran has a milling yield of 80 to 100%. 3. The dietary fiber content is 40% or more, and the content of hemicellulose in the total dietary fiber content is 60%.
The barley-derived inhibitor of gastrointestinal tract function improvement and cholesterol elevation inhibitor according to claim 1 or 2, which is described above. 4. The gastrointestinal tract function derived from barley according to any one of claims 1 to 3, wherein the barley bran is classified by a sieve or the like, and the particle size is classified as passing through a 60M sieve (mesh size: 0.25 mm). An improvement and cholesterol elevation inhibitor. A health food comprising the barley-derived gastrointestinal tract function improving and cholesterol elevation inhibitor according to any one of claims 1 to 4 in a food in an amount of 13% or more.