JPH10108897A - Cleaning and sterilizing method for contact lens - Google Patents

Abstract

PROBLEM TO BE SOLVED: To make it possible to clean and sterilize contact lenses without adverse effects on the contact lenses by bringing the lenses into contact with a process solution containing iodine, ethylenediaminetetraacetic acid or its soluble salt, and a surface-active agent in specific concentrations respectively. SOLUTION: A process solution is used in a cleaning-sterilizing method for contact lenses, which solution containing 0.01-200ppm (preferably 0.1-50ppm) of iodine, 0.0001-1.0 (preferably 0.001-0.5, more preferably 0.001-0.1)% by weight/ volume of ethylenediaminetetraacetic acid or its soluble salt, and 0.01-1.0 (preferably 0.05-0.8, more preferably 0.1-0.5)% by weight/volume of nonionic surface-active agent. As the ethylenediaminetetraacetic acid or its soluble salt may be named; ethylenediaminetetraacetic acid disodium salt, ethylenediaminetetraacetic acid trisodium salt, and ethylenediaminetetraacetic acid tetrasodium salt.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention belongs to the technical field of cleaning and disinfecting a contact lens by immersing it in a specific aqueous solution.

[0002]

2. Description of the Related Art When a contact lens (hereinafter, also referred to as a lens) is worn, a tear component, for example, a protein or an eye lipid, adheres, and oil stains from a finger adhere when attaching or detaching the lens. I do. If such dirt is left as it is, not only the wearing feeling such as itching and foreign body feeling deteriorates when the lens is worn, but also eye disorders such as corneal epithelial disorder and conjunctival congestion occur. In addition, during storage of the lens, bacteria and the like attached to the surface of the lens may grow, and there is a risk that the bacteria may cause an eye infection or the like.

Therefore, in order to wear the lens safely and comfortably, it is necessary to periodically perform lens care such as cleaning and disinfection.

[0004] The method generally used so far is a method in which a lens removed from the eye is rubbed and washed with a detergent containing a surfactant, then immersed in a case filled with a preservative solution, and stored in that state. . In the case of non-hydrous lenses,
A method has been adopted in which a proteolytic enzyme is contained in this preservative solution, the protein is removed, and the rinsing solution is thoroughly rinsed with tap water before use. On the other hand, in the case of a water-containing lens, daily disinfection is indispensable because bacteria and the like attached to the lens surface may proliferate. (Every week), the protein is removed with a washing solution containing protease, and then the method of boiling and disinfecting is carried out, and the storage solution is made to contain proteinase and the removal and disinfection of the protein by boiling and disinfection is performed once. And a method of doing so has been proposed.

[0005] As described above, lens care is complicated, and several kinds of liquids and equipment such as a cleaning solution, a preservative solution, and a boiling disinfectant must be prepared. It is a big burden for the people.

[0006] As a simpler lens care method for solving the above problems, in recent years, a surfactant, a protein remover and a disinfectant have been added to a preservative solution to perform all lens care with a single solution. A possible way has also been proposed. By using such a liquid preparation, especially in the case of a water-containing lens, conventional boiling disinfection is not required, and the labor for care for the lens user is greatly improved.

[0007] The liquid is used not only as a disinfectant but also as a preservative, so that it has a high disinfecting effect, and is worn while the liquid is attached to or contained in the lens. High security is also important.

[0008] As such a liquid preparation (disinfectant), the one using a biguanide derivative which is a high-molecular disinfectant is most commonly used. The reason for using high-molecular disinfectants is that when low-molecular-weight disinfectants such as methylol, chlorhexidine, and benzalkonium chloride are used when disinfecting a water-containing lens, these low-molecular-weight disinfectants penetrate or excessively enter the lens. This is because it has been found that it is adsorbed to the concentration and allergy is likely to occur when the lens is worn.

[0009] On the other hand, it is well known that iodine is used as a disinfectant, and is used in various forms for disinfection of fingers, skin, wound sites, mucous membranes and the like. It is also used for gargles by increasing the stability of iodine by using polymers such as polyvinylpyrrolidone and polyvinyl alcohol as carriers, and reducing its vapor and odor. Has been studied quite a bit.

[0010] As a material used for a contact lens, an aqueous solution containing iodine, a water-soluble iodide, polyvinyl alcohol and boric acid (JP-A-50-1575)
No. 16 publication) is known. After disinfecting the lens with the aqueous solution, the lens is disinfected by dispersing the remaining iodine with sorbic acid and ethylenediaminetetraacetic acid or a soluble salt thereof.

[0011]

However, when a solution containing the above-mentioned biguanide derivative is used as a disinfectant, its use concentration is kept low to enhance safety, and its disinfecting effect is higher than that of boiling disinfection. There is a concern that the lens may be contaminated by bacteria.

When an aqueous solution containing iodine is used, it takes a long time to dissipate the remaining iodine, and there is a problem that iodine tends to stain the lens.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned circumstances, and has a problem to be solved. The present invention has an excellent disinfecting effect and has no adverse effects such as adsorption of a disinfectant on a lens and stains. Another object of the present invention is to provide a cleaning and disinfecting method capable of detoxifying the disinfectant after completion of the disinfecting step and at the same time removing dirt such as eye lipids adhering to the lens.

[0014]

Means for Solving the Problems In order to solve the above problems, the present inventors have conducted intensive studies and as a result, have been found that iodine having an excellent disinfecting effect and having an excellent safety under specific conditions. As a result, the present inventors have found a method that can be used safely on the eyes without adversely affecting the lens, and that allows easy operation of lens care, thereby completing the present invention.

That is, the present invention provides a method for producing iodine of 0.01 to 2
A treatment liquid containing 0.00ppm, 0.0001 to 1.0 w / v% of ethylenediaminetetraacetic acid and / or a soluble salt thereof and 0.01 to 1.0 w / v% of a nonionic surfactant and a contact lens are made transparent. A method for cleaning and disinfecting a contact lens, wherein the contact lens is placed in a case and brought into contact with the contact lens. 2. The method according to claim 1, wherein the treatment liquid is ethylenediaminetetraacetic acid and / or a soluble salt thereof in a transparent case in an amount of 0.0001 to 1.0 w / v% and a nonionic interface. An aqueous solution prepared by adding an iodine solution (II) dropwise to an aqueous solution (I) containing 0.01 to 1.0 w / v% of an activator. Claim contact with the contact lens is performed by the dropwise addition of iodine solution (II) After immersing the contact lens in an aqueous solution (I) 1
The method according to claim 3, wherein the treating solution is ethylenediaminetetraacetic acid and / or in a transparent case.
An aqueous solution prepared by adding an iodine solution (II) dropwise to an aqueous solution (I) containing the soluble salt 0.0001 to 1.0 w / v% and the nonionic surfactant 0.01 to 1.0 w / v%. The method according to claim 1, wherein the contact with the contact lens is performed by dropping the iodine solution (II) at the same time as immersing the contact lens in the aqueous solution (I). Aqueous solution (I) containing 0.0001 to 1.0 w / v% of ethylenediaminetetraacetic acid and / or its soluble salt and 0.01 to 1.0 w / v% of a nonionic surfactant in a transparent case Is an aqueous solution prepared by dropping an iodine solution (II) onto a contact lens. Contact with a contact lens is performed by dropping the iodine solution (II) into the aqueous solution (I) and then immersing the contact lens. Claim 1 is performed by
The method according to claim 5, and the method according to claim 1, 2, 3, 4, or 5, wherein the nonionic surfactant is a polyoxyethylene-polyoxypropylene block copolymer. About.

[0016]

BEST MODE FOR CARRYING OUT THE INVENTION In the method for cleaning and disinfecting a contact lens of the present invention, iodine 0.01 to 200 ppm, ethylenediaminetetraacetic acid and / or a soluble salt thereof 0.0001 to 1.0 w / v%, and nonionic surfactant A treatment solution containing 0.01 to 1.0 w / v% of the agent (hereinafter, also simply referred to as a treatment solution) is used.

The iodine is a component used for the purpose of disinfecting bacteria and the like attached to the lens.

The concentration of iodine in the processing solution is 0.01 to 200 ppm as described above, preferably 0.1 to 10 ppm.
0 ppm, more preferably 0.3 to 50 ppm. If the concentration of iodine is lower than 0.01 ppm, a sufficient disinfecting effect cannot be obtained. If the concentration is higher than 200 ppm, the lens will be colored with iodine even if iodine is dissipated from the aqueous solution after disinfection.

The ethylenediaminetetraacetic acid (EDT)
A) and / or its soluble salt is a component used to dissipate iodine from the treatment solution after washing and disinfection. Specific examples thereof include disodium ethylenediaminetetraacetate, trisodium ethylenediaminetetraacetate, tetrasodium ethylenediaminetetraacetate, and the like.

The ethylenediaminetetraacetic acid and the soluble salt thereof may be used separately or in combination.

The concentration of ethylenediaminetetraacetic acid and / or its soluble salt in the treatment solution is 0.0001.
~ 1.0w / v%, preferably 0.001-0.5w /
v%, more preferably 0.001 to 0.1 w / v%. When the concentration of ethylenediaminetetraacetic acid and / or the soluble salt thereof is lower than 0.0001 w / v%, iodine is difficult to dissipate, and therefore, iodine is colored on the lens after cleaning and disinfection. On the other hand, if it is higher than 1.0 w / v%, the dissolution of iodine occurs too quickly, so that it is difficult to obtain a sufficient disinfection effect.

The nonionic surfactant is used as a carrier for improving the washing properties of the processing solution, removing lipid stains such as eye lipids attached to the lens, and improving the temporal stability of iodine. It is a component to be performed. Since the treatment liquid contains a nonionic surfactant, a part of iodine disappears, but if iodine is further added, iodine does not disappear immediately. In addition, the iodine that is no longer lost is relatively stable unless exposed to light, so that even if the aqueous solution contains ethylenediaminetetraacetic acid and / or a soluble salt thereof, the iodine is less likely to be lost.

It is not clear why part of the iodine disappears and then the added iodine hardly disappears and exists relatively stably. However, the interaction between iodine and the nonionic surfactant is strong, and the iodine has a strong interaction. It is considered that a part of the reaction disappears due to the reaction with the nonionic surfactant, but the reaction does not proceed until the reaction, and only a strong interaction occurs. Since the amount of iodine that is lost varies depending on the type of nonionic surfactant, it cannot be determined unconditionally. For example, as a polyoxyethylene-polyoxypropylene block copolymer, Pluronic F-127 (manufactured by Asahi Denka Kogyo KK) )), The amount is 0.1 g / g.
013 g of iodine disappears, but almost no iodine disappears when polyoxyethylene lauryl ether is used. Therefore, it is preferable to add an extra amount of this iodine according to the amount of iodine that disappears. Adjustment is particularly preferred when the iodine concentration is low. When actually preparing a treatment liquid, iodine may be added to an aqueous solution containing a nonionic surfactant, and the amount added after the solution no longer disappears may be the amount of iodine added.

Examples of the nonionic surfactant include polyoxyethylene-polyoxypropylene block copolymer, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene alkyl ether, and polyoxyethylene stearate. Of these,
Polyoxyethylene which is physiologically compatible with the eye in that it is highly safe without irritation or sensitization even when it is directly injected into rabbit eyes at a concentration of about 10%.
Polyoxypropylene block copolymers are preferred. As a specific example, Pluronic F-68, which is commercially available from Asahi Denka Kogyo KK under the trade name of "Pluronic",
Examples include Pluronic L-64, Pluronic L-62, Pluronic L-72, Pluronic F-108, Pluronic F-127, etc., and Pluronic F-127, which is often used as a lens cleaning agent, is preferable.

The concentration of the nonionic surfactant in the treatment solution is 0.01 to 1.0 w / v%, preferably 0.05 to 1.0 w / v%.
0.8 w / v%, more preferably 0.1 to 0.5 w / v
v%. When the concentration of the nonionic surfactant is 0.0
When the content is lower than 1 w / v%, the stabilization of iodine is insufficient, so that the iodine is rapidly eliminated, the disinfecting effect is insufficient, and the effect of removing lipid stains attached to the lens is reduced. I do. On the other hand, if it is higher than 1.0 w / v%, the amount of the nonionic surfactant required for washing is used in excess, which is not only uneconomical, but also loses iodine due to the nonionic surfactant. The volume is increased and this is also uneconomical.

Further, although iodine is lost by light and ethylenediaminetetraacetic acid and / or its soluble salt, the reaction is probably I ₂ → 2I ⁻ , and I ⁻ remains in the aqueous solution. The I ^- since return to the original I ₂ by air oxidation, iodine concentration in the aqueous solution will be low it is preferable.
Therefore, the amount of the nonionic surfactant used is preferably set in the above range.

In addition to the above components, the pH of the processing solution
A borate buffer, a phosphate buffer, and the like may be contained for stabilizing the compound.

The concentration of the buffer in the treatment solution is generally 0.05 to 3.0 w / v%, preferably 0.1 to 3.0 w / v%.
１．５1.5 w / v%. When the concentration of the buffer is 0.05
If it is lower than w / v%, it will be difficult to maintain the desired pH, and if it is higher than 3.0 w / v%, it will only be used in excess of the amount necessary for stabilizing the pH.

It is preferable to use an isotonic agent (sodium chloride, potassium chloride, etc.) in the treatment liquid in order to reduce irritation to the eyes. Generally 150 ~
About 400 mOsm, preferably 200 to 350 mOm
It may be used so that the osmotic pressure becomes about s.

The pH of the treatment solution prepared from the above components is preferably in the range of 6.0 to 8.0 which does not cause any damage to the eyes.

The method for preparing the treatment liquid is not particularly limited,
As a preferable preparation method, iodine is directly dissolved in water separately from an aqueous solution (I) in which ethylenediaminetetraacetic acid and / or a soluble salt thereof and a nonionic surfactant and, if necessary, a buffering agent and an isotonic agent are dissolved. Alternatively, there is a method in which an iodine solution to which potassium iodide or the like is added for improving the water solubility is prepared, and after the user purchases, the two solutions are mixed to prepare a treatment solution. In this case, there is an advantage that the loss of iodine in the distribution stage is reduced.

As a method for preparing the aqueous solution (I), the concentration of ethylenediaminetetraacetic acid and / or its soluble salt is 0.0001 to 1.0 when mixed with an iodine solution.
w / v%, and the concentration of the nonionic surfactant is 0.0
It is preferable to prepare an aqueous solution by mixing necessary components so as to be 1 to 1.0 w / v%.

The method for preparing the iodine solution is such that when mixed with the aqueous solution (I), the iodine concentration is 0.01 to 2%.
The concentration is 10 to 1000 ppm by mixing iodine and water or a solution obtained by adding iodide such as potassium iodide for improving the water solubility of iodine to water so as to be 00 ppm.
Further, it is preferable to prepare an aqueous solution so as to have a concentration of 100 to 300 ppm. The concentration of iodide used to improve the water solubility of iodine should be kept as low as possible.
This is preferable because generation of iodine due to air oxidation and the resulting coloring can be suppressed.

In the present invention, the treatment liquid is put into a transparent case at the time of use, and the contact lens is immersed in the case to clean and disinfect the contact lens.

The transparent case may be a case where ordinary room light, preferably light having a wavelength of 350 to 900 nm, can be transmitted by 40% or more, preferably 60% or more. In order to clean and disinfect the lens by placing the processing solution in such a case, the stabilized iodine combined with the nonionic surfactant is exposed to light and ethylenediaminetetraacetic acid and / or a soluble salt thereof in the processing solution. As a result, iodine is not colored on the lens, and a sufficient disinfecting effect can be obtained. When the permeability of the case is low, iodine tends to remain in the aqueous solution, which tends to cause coloring of the lens.

As the material of the case, general-purpose plastics such as polycarbonate, polymethyl methacrylate, polyethylene,
Polypropylene, polystyrene and the like can be adopted.

The shape of the transparent case may be any shape, but a preferable shape is one having a light transmitting portion on the upper surface and / or the side surface when the case is placed.

The method for cleaning and disinfecting a contact lens using a treatment liquid can be specifically carried out by the following procedure.

First, a normal cleaning agent or preservative is dropped on the lens removed from the eye, and the lens is placed between the thumb and the forefinger.
Alternatively, while holding on the palm, scrub for several seconds to several tens of seconds. Next, the lens is rinsed with the above-mentioned cleaning agent or preservative, and then the lens is immersed in a transparent case containing a processing solution. The lens can be washed and disinfected by immersing the lens in this processing solution for several minutes to four hours, preferably several minutes to one hour.

The cleaning and disinfecting of the lens is preferably carried out with the brightness of general room light.

By cleaning and disinfecting the lens by the above-described method, the user purchases the aqueous solution (I) and the iodine solution, then only mixes the aqueous solution (I) and the iodine solution at a time, and then the processing solution. It can be used as it is until there is no more. That is, the trouble of dropping the iodine solution every time the lens is processed can be omitted.

In the above method, iodine is used in an amount of 0.01 to 2
A processing solution containing 00 ppm, 0.0001 to 1.0 w / v% of ethylenediaminetetraacetic acid and / or a soluble salt thereof, and 0.01 to 1.0 w / v% of a nonionic surfactant was used. An aqueous solution (aqueous solution (I)) prepared by removing a stable iodine in the solution is prepared,
Iodine may be added each time it is used. In this case, the drawback that the stability of iodine is reduced after the preparation of the treatment liquid can be improved. Further, the aqueous solution (I) can be used as a detergent or a preservative without preparing a detergent or a preservative separately. The iodine to be added is
From the viewpoint of handling and safety, iodine concentration is 10 to 1000
ppm or even 100 to 300 ppm of iodine directly in water, or an aqueous solution to which potassium iodide or the like for improving water solubility is added (an iodine solution (II) or an aqueous solution (I)) (The same as the above-mentioned iodine solution) is preferably used.

The addition of the iodine solution (II) may be performed before the lens is immersed in the aqueous solution (I), at the same time as the lens is immersed, or after the immersion. It is preferable to add (II) easily, and to add the iodine solution (II) after the lens is immersed, since iodine hardly penetrates into the inside of the lens and the lens is hardly colored. In any case, it suffices that the lens finally comes into contact with the processing liquid and the lens is cleaned and disinfected.

The iodine solution (II) may be prepared in the same manner as the iodine solution used as a treatment liquid by mixing with the aqueous solution (I).

When the lens is washed and disinfected using the aqueous solution (I) and the iodine solution (II), the iodine concentration in the aqueous solution is 0.01 to 200 ppm, preferably 0.1 to 200 ppm.
1 to 100 ppm, more preferably 0.3 to 50 pp
m. When the iodine concentration in the aqueous solution is lower than 0.01 ppm, a sufficient disinfecting effect cannot be obtained, and when the iodine concentration is higher than 200 ppm, it takes a long time to dissipate iodine from the aqueous solution after disinfection. Becomes colored with iodine.

When the iodine solution (II) having the above concentration is added dropwise to 5 ml of the aqueous solution (I) in an amount of 0.005 to 1.25 ml, preferably 0.06 to 1.0 ml, an aqueous solution within the iodine concentration range can be obtained. Can be

The method for cleaning and disinfecting a contact lens using the aqueous solution (I) and the iodine solution (II) can be specifically carried out by the following procedure.

First, the aqueous solution (I) was applied to the lens removed from the eye.
And hold the lens between the thumb and forefinger, or on the palm, and scrub for several seconds to several tens of seconds. Then, after rinsing the lens with the aqueous solution (I), before dropping the iodine solution (II) into the aqueous solution (I) in a transparent case, simultaneously with dropping the iodine solution (II), or simultaneously with the iodine solution (II). ) Is dropped, and then the lens is immersed. The lens can be washed and disinfected by immersing the lens in this aqueous solution for several minutes to four hours, preferably several minutes to one hour.

The above-mentioned cleaning and disinfecting method is preferably performed at the brightness of general room light.

[0050]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, which by no means limit the present invention.

Preparation Examples 1 to 4 Preparation of Aqueous Solution (I) Using the following four nonionic surfactants, iodine-free aqueous solutions a to d having the compositions shown in Table 1 were prepared.
Purified water was used for the preparation of all aqueous solutions. Types of nonionic surfactants 1 Pluronic F-127 (trade name of polyoxyethylene-polyoxypropylene block copolymer manufactured by Asahi Denka Kogyo KK) 2 Polyoxyethylene lauryl ether (common name: B
rij35) (manufactured by Wako Pharmaceutical Co., Ltd., polyoxyethylene alkyl ether) 3 polysorbate 80 (manufactured by Nikko Chemical Co., Ltd., trade name of polyoxyethylene sorbitan fatty acid ester) 4 polyethylene glycol monostearate (manufactured by Tokyo Chemical Industry Co., Ltd.) , Polyoxyethylene stearate)

The pH of the obtained aqueous solution and the osmotic pressure according to the following method were measured. Table 1 shows the results.

(Osmotic pressure) The osmotic pressure of the obtained aqueous solution is p
It measured using H-osmometer (HOSM-1) and Toa Denpa Kogyo.

[0054]

[Table 1]

Examples 1 to 4 4 ml each of the aqueous solutions a to d obtained in Production Examples 1 to 4.
Was placed in a transparent case made of polystyrene (transmittance of light having a wavelength of 700 nm of about 70%), and 0.1 ml of a 290 ppm aqueous iodine solution was added to prepare treatment solutions 1 to 4 having an iodine concentration of 7.07 ppm.

The following microbial tests were performed on the obtained treatment solutions 1 to 4. Table 2 shows the results.

(Microbial test) 10 ³ Aspergillus nigers were added to each of the treatment liquids 1-4.
ger (A. n.)), two lenses (trade name: Menicon Soft 72, manufactured by Menicon Co., Ltd.) were put into each processing solution, immersed in room light for 15 minutes, and then taken out of the lens. The number of surviving bacteria was measured.

At this time, the time until the iodine disappeared was about 10 minutes.

[0059]

[Table 2]

In Table 2A. n. It can be seen from the results of the number of surviving bacteria that the disinfecting effect is higher in the order of treatment liquid numbers 1>2> 3 = 4.

Among the above-mentioned treatment solutions, the treatment solution 1 having the highest disinfecting effect was further subjected to the following safety evaluation test.
Table 3 shows the results.

(Safety Evaluation Test) The safety was evaluated by determining the growth inhibition rate according to the following procedure. How to determine the growth inhibition rate a 1 × 10 ⁵ cells / ml of cell suspension was
-Prepared in ale medium. b. Cell suspension prepared in 96-well plate
1 ml was dispensed, and 2-3% at 37 ° C. in a 5% CO 2 incubator.
After culturing for a time, it was confirmed that the cells stuck to the bottom of the well. c 0.01 ml of test solution (control or treatment solution 1) was added. d The cells were cultured in a 5% carbon dioxide incubator at 37 ° C. for 3 days. e The solution in the plate was discarded and washed twice with 0.2 ml of PBS (-) repeatedly. 0.025 ml of f1N NaOH was added and stirred. g 0.1 ml of alkaline copper solution was added and stirred. h Left at room temperature for 10 minutes. i 0.01 ml of phenol solution was added and stirred. j Let stand at room temperature for 30-60 minutes. The k sample was measured for absorbance at a wavelength of 655 nm using an absorptiometer (microplate reader (Model 450, manufactured by Japan Bio-Rad Laboratories Co., Ltd.). The growth inhibition rate was determined by the following formula: Growth inhibition rate (%) ) = (Absorbance of control−absorbance of sample) /
Control absorbance x 100

The reagent used in the safety evaluation test was
The media and reagents prepared therefrom, the test solution, the cells used and the culture conditions thereof, and the method for preparing the cell suspension will be described later.

[0064]

[Table 3]

From the results in Table 3, it can be seen that the treatment liquid 1 returns to a safe solution within 15 minutes after the addition of iodine.

(Reagents Used in Safety Evaluation Test, Medium and Reagents Prepared Therefrom, Test Solutions, Cells Used, Culture Conditions for the Cells, and Method for Preparing Cell Suspension) 1 Reagent MEM-Earle Medium Life Technology ( L-Glutamine (200mM) Dainippon Pharmaceutical Co., Ltd. Sodium hydrogen carbonate Reagent Special grade Wako Chemical Co., Ltd. Fetal bovine serum Life Technology Co., Ltd. SALTS) 0.05% Trypsin 0.53mM EDTA / 4Na Sodium carbonate (anhydrous) manufactured by Life Technology Co., Ltd. Reagent special grade Wako Chemical Co., Ltd. Copper sulfate (II) pentahydrate Reagent special grade Wako Chemical Co., Ltd. Hydroxide Sodium reagent special grade Wako Chemical Co., Ltd. Sodium citrate reagent Wako Chemical Co., Ltd. phenolic reagent Wako 2 media and reagents manufactured by Chemical Co., Ltd. ・ Prepared MEM-Yale medium Eagle's minimum essential medium (Mi
minimum Essential Medium (ME
M)) in a medium containing a balanced salt solution of ale (MEM-Ale medium), L-glutamine (2 mM), sodium bicarbonate (2.2 g / l or less) and 10 fetal bovine serum
(V / v%).・ Phosphate buffer solution (PBS (-)): phosphate
Dissolve one buffered salt in 100 ml of purified water and sterilize by high pressure steam. Composition: NaCl 8.0 g / l, KCl 0.2 g /
1, Na ₂ HPO ₄ 0.2 g / l pH: 7.2 Trypsin solution 0.05% Trypsin 0.53 mM EDTA-4
Na · Alkaline copper solution (prepared when only mixing is used) 2% Na ₂ CO ₃ aqueous solution: 0.5% CuSO ₄ .5H ₂ O aqueous solution: 1% sodium citrate aqueous solution = 50: 1: 1
(Volume ratio) 1N NaOH Phenol solution (prepared at the time of use) A phenol reagent diluted twice with purified water. In addition,
Reagents and media were prepared and stored according to the manufacturer's instructions. Purified water was used for all preparations. 3 Control (a solution that is a standard with no toxicity) Control: PBS (-) 4 Cells used Cells used Name: Mouse connective tissue cell derived (CCL1) (hereinafter referred to as L929) Supplier: RIKEN Gene Bank Cell Bank 5 cells Preparation Method of Suspension After confirming that the cells grew to a monolayer in the culture flask and were in a state close to saturation, the culture solution in the culture flask was removed, and the cells were washed with PBS (−). After treatment with a trypsin solution to remove the cells, the cells were suspended in an appropriate amount of MEM-ale culture solution, the number of cells was counted, and a cell suspension of a required concentration was prepared.

Example 5 For treatment solutions 1, 2, 3 and 4, A.I. n. Instead of Candida albicans
cans (C.a.)), Pseudomonas aeruginosa (Pseudomonas aerginosa)
(Pa)), Staphylococcus aureus (S
tapylococcus aureus (St.
a. )) And Serratia marcesens (Serrat)
ia marcescens (Sm.)), the number of surviving bacteria after the test was 1
In 2, 3, and 4, all were 0. This is probably because bacteria other than mold are weak against iodine.

Examples 6 to 9 The EDTA concentration in the aqueous solution 1 obtained in Production Example 1 was adjusted to 0.001, 0.002, 0.005, 0.01 w / v.
% Aqueous solutions e to h were prepared in the same manner as in Production Example 1 except that the amount was changed to%.

Polycarbonate transparent (wavelength 700n)
5 ml of each aqueous solution in a case where the light transmittance of
Then, 0.8 ml of a 290 ppm iodine solution was added to prepare treatment solutions 5 to 8 having an iodine concentration of 40 ppm.

[0070] The obtained processing solution 5-8 in 10 ³ C.
a. (Manufactured by Menicon Soft 72, manufactured by Menicon) were immersed and allowed to stand still under room light. The time until the iodine disappeared and the number of surviving bacteria after 2 hours were measured. Table 4 shows the results.

When the EDTA concentration is 0.001 w / v%
In the case of the treatment liquid 5, coloring was observed in the lens due to the long time until the iodine disappeared, but this coloring disappeared after one night.

[0072]

[Table 4]

From Table 4, no survival of the bacteria was confirmed from any of the lenses, indicating that the lens has a high disinfecting effect.

[0074]

According to the method for cleaning and disinfecting contact lenses of the present invention, a high disinfecting effect on bacteria can be obtained because iodine is used as a disinfectant. Further, since a transparent case is used, iodine can be dissipated by light after disinfecting the lens, and there is no problem if the treated lens is worn as it is. In addition, since the solution contains a nonionic surfactant, lipid stains can be removed from the lens simultaneously with disinfection.

Claims (6)

[Claims] 1. The composition contains 0.01 to 200 ppm of iodine, 0.0001 to 1.0 w / v% of ethylenediaminetetraacetic acid and / or a soluble salt thereof, and 0.01 to 1.0 w / v% of a nonionic surfactant. A method for cleaning and disinfecting a contact lens, comprising placing a treatment solution to be contacted with a contact lens in a transparent case. 2. The method according to claim 1, wherein the contact between the treatment liquid and the contact lens is performed by immersing the contact lens in the treatment liquid in a transparent case. 3. The treatment liquid contains ethylenediaminetetraacetic acid and / or a soluble salt thereof in a transparent case in an amount of 0.0001 to 1.0 w / v% and a nonionic surfactant of 0.01 to 1.0 w / v. An aqueous solution prepared by dropping an iodine solution (II) into an aqueous solution (I) containing v%, and contacting with a contact lens is performed by immersing the contact lens in the aqueous solution (I) and then dropping the iodine solution (II). 2. The method of claim 1, wherein the method is performed. 4. The treatment liquid contains ethylenediaminetetraacetic acid and / or a soluble salt thereof in a transparent case in an amount of 0.0001 to 1.0 w / v% and a nonionic surfactant in an amount of 0.01 to 1.0 w / v. An aqueous solution prepared by dropping an iodine solution (II) into an aqueous solution (I) containing the iodine solution (I) at the same time when the contact lens is immersed in the aqueous solution (I).
The method according to claim 1, which is carried out by dropping I). 5. The treatment liquid contains ethylenediaminetetraacetic acid and / or a soluble salt thereof in a transparent case in an amount of 0.0001 to 1.0 w / v% and a nonionic surfactant of 0.01 to 1.0 w / v. An aqueous solution prepared by dropping an iodine solution (II) into an aqueous solution (I) containing v%, and contacting with a contact lens is performed by dropping the iodine solution (II) into the aqueous solution (I) and then immersing the contact lens 2. The method of claim 1, wherein the method is performed. 6. The method according to claim 1, wherein the nonionic surfactant is a polyoxyethylene-polyoxypropylene block copolymer.