JPH0951778A - Alcohol-decomposing composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an alcohol-decomposing composition having strong alcohol- decomposing action, decomposing alcohol inside the stomach and preventing excess alcohol absorption by freeze-drying an acetic acid bacterium decomposing alcohol below a specific acidic pH and mixing the dried microbes therein. SOLUTION: An acetic acid bacterium decomposing alcohol below pH3 (e.g.; Acetobacter aceti IFO 3281, etc.) is cultured and separated to the culture medium and the bacterial cells by centrifugation, to collect the cells. The cells are freeze- dried and made to powder, and the powdery material is formulated to a powder agent, a tablet, a capsule agent, a granule, a liquid agent or a drink, etc., by adding additives such as an extending agent, an excipient, etc., according to shapes of the formulation products to obtain the objective alcohol-decomposing composition. The composition has a strong alcohol-decomposing action and when taken in drinking of alcohol or before the drinking, alcohol is oxidized to acetic acid through acetaldehyde by the acetic acid bacterium inside the stomach, thus excess alcohol absorption is prevented.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an alcohol-decomposable composition containing acetic acid bacteria, which is administered at the time of or before ingesting an alcoholic beverage to decompose the alcohol in the stomach to produce excess alcohol. The present invention relates to an alcohol-decomposable composition capable of preventing absorption. In this specification, the term "alcohol" means
Unless otherwise specified, it means ethyl alcohol.

[0002]

2. Description of the Related Art Alcoholic beverages are delicious, have excellent properties such as relaxing work tension and brightening mood, and are widely used in family gatherings, social gatherings such as dinners and banquets, and the like. However, symptoms such as drunkenness, sickness, and hangover appear with an increase in the alcohol concentration in the blood, such as when alcohol is excessively ingested or when a person with a constitution weak to alcohol drinks socially. Various drugs have been developed to alleviate such symptoms.
There are alcohol absorption inhibitors disclosed in JP-A-3-127739, alcohol metabolism promoters disclosed in JP-A-4-124133, and JP-A-49-132286 discloses that the stomach and the like An enzyme solution of yeast, which decomposes alcohol in the digestive tract, is disclosed. In particular, although this enzyme solution directly decomposes alcohol, the alcohol dehydrogenase and aldehyde dehydrogenase contained in this enzyme solution exert their activity only in the alkaline region of pH 7 or higher,
It has no effect on the stomach, which secretes acidic digestive juices, and is almost inactivated during the transfer from the stomach to the intestine. Therefore, the enzyme solution of yeast has a problem in its effectiveness.

[0003]

DISCLOSURE OF THE INVENTION The present invention provides an alcohol-decomposable composition which can be administered at or before ingestion of an alcoholic beverage to decompose alcohol in the stomach and prevent excessive alcohol absorption. The purpose is to do.

[0004]

[Means for Solving the Problems] Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) possessed by ordinary yeasts are active on the alkaline side at a pH of about 7.5 to 10.0, and thus are acidified. Alcohol cannot be broken down inside the stomach. In addition, the alcohol-metabolizing enzyme system containing the membrane-bound ADH and ALDH of acetic acid bacteria has an optimum pH of around 4, and has almost no activity at pH 3 or less, and therefore cannot sufficiently decompose alcohol in the stomach. Was being considered. On the other hand, the present inventors have discovered that the cell suspension itself before extraction of the membrane-bound ADH and ALDH of acetic acid bacteria exhibits an enzymatic activity of oxidizing alcohol even at pH 3 or lower. It has been found that if acetic acid bacteria having a characteristic is ingested during or before drinking alcohol, the alcohol is oxidized in the stomach via acetaldehyde to acetic acid by the acetic acid bacteria and the alcohol is not absorbed in the digestive tract. The present invention has been made based on such findings, and therefore the present invention provides p
It is intended to provide an alcohol-decomposable composition containing acetic acid bacteria that decomposes alcohol in an acidic region of H 3 or less. Hereinafter, the contents of the present invention will be described in detail.

In the present invention, acetic acid bacteria can be used without particular limitation as long as they decompose alcohol at pH 3 or less and are harmless to the human body. Examples of preferable acetic acid bacteria that can be used in the present invention are as follows. The IFO number is a reference number given to the deposited bacteria of the Fermentation Research Institute. Acetobacter aceti IFO 3281, IFO 3283, IFO 3284 Acetobacter acetigenus IFO 3279 Acetobacter acetosus IFO 3296 Acetobacter ascendens IFO 3289 Acetobacter aurantius IFO 3245, IFO 3247, IFO 3248 Acetobacter kutzingianus IFO 3222 Acetobacter rances IFO 3297, IFO 3298 Acetobacter xylinus IFO 3298 Pasteurianus IFO 3223 Acetobacter turbidans IFO 3225

Gluconobacter genus Gluconobacter melanogenus IFO 3294 Gluconobacter oxydans IFO 3287 Gluconobacter dioxyacetonicus IFO 3271, IFO 3272, IFO 3274 Gluconobacter Gluconobacter cerinus IFO 3262, IFO 3263, IFO 3264 IFO 3265, IFO 3266, IFO 3267 IFO 3268, IFO 3269, IFO 3270 Gluconobacter gluconicus IFO 3285, IFO 3286 Gluconobacter lucon arubidas albidus) IFO 3251, IFO 3253 Gluconobacter suboxydans IFO 3290, IFO 3291, IFO 12528

In the present invention, either acetic acid bacteria that can grow (hereinafter referred to as active acetic acid bacteria) or acetic acid bacteria that cannot grow (hereinafter referred to as inactive acetic acid bacteria) may be used. When using active acetic acid bacterium, after culturing the acetic acid bacterium, separating the medium and acetic acid bacterium by centrifugation and collecting the bacterial cells as they are, or suspending the collected bacterial cells in an aqueous solution before use Or a method in which the collected cells are freeze-dried to be powdered.
Examples of the method for preparing the inactive acetic acid bacterium include a method of heat-treating the acetic acid bacterium at 40 to 60 ° C. for 30 to 60 minutes and a method of mechanically or ultrasonically disrupting the acetic acid bacterium. With the alcohol-decomposable composition of the present invention, the intake amount for obtaining the action of lowering the alcohol concentration in the stomach is very different depending on the individual weight and constitution of the person who ingests it, and the amount of alcohol consumed is large. Although it cannot be clearly specified because it influences, in order to fully exert the decomposition effect of alcohol in the stomach, the amount of one intake is in the range of 0.01 to 25 g, preferably 0.05 to 5 g. Is desirable.

The composition of the present invention may be in the form of powder, tablets,
It may be any of capsules, granules, liquids, beverages and the like. Further, depending on the form of the composition, a bulking agent, an excipient,
Add additives such as. It can also be used as a beverage. As an example, the pH is adjusted to 3.5 to 4.0 by adding 0.05 to 5 g of the composition to 10% sugar, 0.1% sourness, 0.1% fruit juice 2-3% flavor. Then, heat sterilization at 65 ° C. for 10 minutes is preferable. It is preferable to refrigerate the obtained product before drinking.

[0009]

The alcohol-decomposable composition of the present invention is
Alcohol and acetaldehyde are rapidly decomposed even at H 3 or less, so that excessive alcohol absorption is prevented. In addition, the acetic acid bacterium of the present invention is a conventional membrane-bound ADH and A.
Since it also contains LDH, it strongly decomposes alcohol when the pH inside the stomach reaches pH 3 or higher. Therefore, the alcohol-decomposable composition of the present invention has a strong alcohol-decomposing effect and prevents excessive absorption of alcohol.

[0010]

【Example】

(Example 1) The relationship between the ADH activity and the ALDH activity of acetic acid bacteria and pH was examined. Acetobacter aceti (IF
O 3284) washed cells were uniformly dispersed in 20 ml of distilled water and freeze-dried to obtain a cell powder. Both enzyme activities are W
This was carried out according to the method of using ferricyanide as an electron acceptor by Ood et al. The reduction ratio of ferricyanide to ferrocyanide corresponds to the amount of oxidized alcohol and the amount of acetaldehyde. Reaction composition is 10μ
mol potassium ferricyanide, 0.5 ml Mc at various pH
Ilvaine's buffer, 100 μmol alcohol or 20 μmol acetaldehyde, and acetic acid bacteria powder
A bacterial solution containing 0.1 mg was added to make a total volume of 1.0 ml. The reaction was started by adding potassium ferricyanide and carried out at 37 ° C. for 20 minutes. Immediately, 0.5 ml of Dupanol solution was added to stop the enzymatic reaction. Then, 3.5 ml of distilled water was added, mixed well, and allowed to stand at room temperature for 20 minutes, and then Prus produced.
The sisn blue color was measured at 660 nm. The activity was expressed as 1 unit of the activity when 1 μmol of alcohol or acetaldehyde was decreased in 1 minute. Experimental results, Figure 1
As shown in, the optimum pH of ADH of acetic acid bacteria is around 4,
In the case of DH, it is around pH 5, but at pH 3, ADH and ALDH show almost no activity.

Example 2 An experiment was carried out to examine the relationship between pH and the enzyme activity involved in alcohol oxidation of acetic acid bacteria. The experimental procedure was as follows. The activity of the enzyme system involved in the oxidation of alcohol was measured from the rate of oxygen consumption using a dissolved oxygen meter. That is, McIlvaine's buffer solution 4.6 of various pH was added to a 10 ml beaker (2.5 × 5 cm).
ml, 0.2 ml of a bacterial solution containing 1 mg of the acetic acid bacterium powder prepared in Example 1 was added, and then 1.2 ml of 1.0 M alcohol was added to carry out an enzymatic reaction at 25 ° C. The activity was indicated by consuming 1 μmol of oxygen per minute as one unit. As a result, the enzymatic activity involved in the oxidation of alcohol shows an optimum pH around pH 4, but as shown in the graphs of Table 1 and FIG.
At 0%, 33% at pH 2.5 and 68.8% at pH 3.0
Met. Example 1 As can be seen from Example 2, the enzyme activity involved in the oxidation of alcohol has a strong alcoholysis activity in a low acidic region of pH 3 or less, as compared with the ADH and ALDH activities of acetic acid bacteria. Therefore, it can be seen that the enzyme system involved in the oxidation of alcohol contained in the acetic acid bacterium powder sufficiently acts even in the stomach having a low pH.

[0012]

[Table 1] Table 1 Relationship between alcoholysis activity and enzyme activity of acetic acid bacteria ────────────────────────────────── pH 1.5 2.0 2.5 3.0 4.0 Enzyme activity 10.8 21.5 495.1 1033.2 1501.4 (Unit / ml) * 0.007% 0.014% 33.0% 68.8% 100.0% * Strength at each pH when the enzyme activity at pH 4.0 is 100%

(Example 3) Next, it is shown in an animal experiment that the alcohol-decomposable composition of the present invention decomposes alcohol in the stomach. Acetobacter aceti (IFO 3284) or
A suspension of 25 mg of freeze-dried cells of Gluconobacter Suboxydans (IFO 12528) in 2.0 ml of water and 2.0 ml of a 10% ethanol solution are collected in separate test tubes. Male Wistar rats (8 to 10 weeks old), which had been fasted for 24 hours before, were anesthetized and subjected to laparotomy, and the respective solutions were mixed for several seconds, and then the entire amount was immediately administered to the stomach with a syringe. Then, after 0 minutes, 5 minutes, 10 minutes, and 15 minutes, about 150 μm of blood was passed through a cannula tube placed in the inferior vena cava.
1 was collected. 0.33 ml for 100 μl of collected blood
Per liter of perchloric acid (800 μl) was added and mixed to obtain 3.0
After centrifugation at 00 rpm for 5 minutes, the amount of ethanol in the supernatant was measured using an ethanol measurement kit (Boehringer Mannheim Co., Ltd.). At the same time, as a control, 2.0 ml of distilled water and 2.0 ml of 10% ethanol solution were administered to the stomach of the rat with a syringe, and the same measurement was performed. Figure 3 shows the measurement results.
Is shown in the graph. As is clear from FIG. 3, it was found that the rat to which the acetic acid bacterium cell suspension was administered had a significantly lower alcohol concentration in blood, and a significant difference was observed at a risk rate of 7%. Therefore, it is clear that when present in the stomach, the alcohol-degradable composition of the present invention decomposes alcohol and suppresses excessive absorption of alcohol.

[Brief description of drawings]

FIG. 1 is a graph showing the relationship between the activity of ADH and ALDH of acetic acid bacteria and pH.

FIG. 2 is a graph showing the relationship between the activity of an enzyme system involved in alcohol oxidation of acetic acid bacteria and pH.

FIG. 3 is a graph showing the blood alcohol concentration in rats administered with an acetic acid bacterium cell suspension.

Claims (2)

[Claims] 1. An alcohol-decomposable composition containing acetic acid bacteria that decompose alcohol at a pH of 3 or less. 2. The alcohol-decomposable composition according to claim 1, wherein the acetic acid bacterium is a freeze-dried acetic acid bacterium.