JPH09299813A - Container for dialysis - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a container for dialysis which enables the dialysis of a small quantity of a sample and can improve handling and reduce costs. SOLUTION: A bottomed cylindrical container main body 11 having an opening 13 through which a sample S to be dialyzed can be put in/out and a lid 12 which opens/closes the opening 13 are provided. A hole 18 with a prescribed size is formed at a position corresponding to the opening 13 of the lid 12, a dialysis film 19 is arranged between the container main body 11 and the lid 12, and when the lid 12 is attached to the container main body 11, the opening 13 and the hole 18 are partitioned by the dialysis film 19. The film 19 can be stuck on the inner surface of the lid 12. The lid 12 can be fixed to the container main body 11 with screws. A micro-tube for a small-sized centrifuge can be used in the container main body 11.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a dialysis container, and more particularly to a small volume dialysis container.

[0002]

2. Description of the Related Art Conventionally, various dialysis containers have been proposed. For example, dialysis tubes are often used when dialyzing a relatively large amount of sample. This dialysis tube is a tube whose both ends are open and is formed of a dialysis membrane. When dialysis treatment is performed using this dialysis tube, one of the openings is closed with, for example, a clip, the sample is injected from the other opening, and then this opening is sandwiched with a clip and closed.

This dialysis tube is not suitable for a small amount of sample because there is a gap that makes it difficult for the sample to enter and be taken out at the portion sandwiched by clips or the like. Therefore, when dialyzing a small amount of sample, another dialysis container has been used.

As such a small capacity dialysis container,
For example, there is one in which two dialysis membranes are laminated in a flat bag shape, the periphery thereof is sealed with an outer frame, and the sample is taken in and out from the outer peripheral end surface of the outer frame into the inner space of the bag with, for example, a syringe.

[0005]

However, the conventional small capacity dialysis container is troublesome to handle, such as using a syringe, and has a problem that the structure is complicated and the manufacturing cost is high. Further, there is a problem that it becomes difficult to process a large number of samples.

An object of the present invention is to solve such a problem, and to provide a dialysis container capable of improving the handling property even in the case of a small volume and reducing the cost. .

[0007]

The present invention is a dialysis container, which has the following structure in order to solve the above-mentioned technical problems. That is, the dialysis container of the present invention includes a bottomed cylindrical container body having an opening through which a sample to be dialyzed can be put in and taken out, and a lid for opening and closing the opening, which corresponds to the opening of the lid. A hole having a predetermined size is provided at a position, a dialysis membrane is arranged between the container body and the lid, and when the lid is attached to the container body, the opening and the hole are partitioned by the dialysis membrane. It is characterized by doing so.

The dialysis container of the present invention comprises a bottomed cylindrical container body having an opening through which a sample to be dialyzed can be put in and taken out, and a lid for opening and closing the opening, and the bottom of the container body. Alternatively, it is characterized in that a hole having a predetermined size is provided on a side portion and the hole is closed by a dialysis membrane. Hereinafter, each component will be described. (Container main body) As the container main body of the dialysis container targeted by the present invention, polypropylene is molded into a bottomed tubular shape,
It is possible to use one having an opening at one end for loading and unloading the sample. (Lid) As the lid, one that can open and close the opening of the container body can be used, and a hole of an appropriate size can be provided at a position corresponding to the opening. (Dialysis membrane) As the dialysis membrane, cellulose, cellulose acetate, polyacrylonitrile, polysulfone, polyether sulfone, polyaramid, or the like can be used. (Additional configuration in the present invention) The dialysis container of the present invention comprises:
It consists of the above-mentioned essential components, but it also holds true when the following components are added to these components. The component is that the dialysis membrane is attached to the inner surface of the lid. The lid may be attached to the container body by screwing, or the lid may be provided with an annular protrusion having elasticity, and the protrusion may be fitted into the opening to attach the lid to the container body. it can. Furthermore, as the container body, a microtube for a small centrifuge can be used.

In the dialysis container of the first invention, when the lid is attached to the container body, the dialysis membrane disposed between the lid and the container body is fixed, and the dialysis membrane causes the opening of the container body and the hole of the lid. And are partitioned so that the sample in the container body can be dialyzed with a dialysis membrane.

In the dialysis container of the second invention,
Since the hole is provided at the bottom or side of the container body and the hole is simply closed with the dialysis membrane, the structure is very simple.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, a dialysis container according to the present invention will be described in detail with reference to the illustrated embodiments. FIG. 1 shows a dialysis container 1 according to the first embodiment of the present invention. The dialysis container 1 includes a bottomed cylindrical container body 11 and a cap-shaped lid 12 that opens and closes an opening 13 of the container body 11. As the container body 11, for example, a polypropylene microtube for a small centrifuge can be used, and the sample S to be dialyzed can be put into and taken out of the container body 11 through the opening 13.

The container body 11 and the lid 12 are detachably coupled by screws 14 and 15 provided on the outer peripheral surface or the inner peripheral surface of the upper end portion, respectively. As a result, the sample S in the container body 11 can be prevented from leaking from the joint between the container body 11 and the lid 12. Container body 11
A collar 16 is provided below the screw 14. Lid 1
A chamfer 17 is provided on the edge of the second screw 15.

The lid 12 is provided with a hole 18 having a predetermined size at a position corresponding to the opening 13 of the container body 11.
A dialysis membrane 19 is arranged between the container body 11 and the lid 12. The dialysis membrane 19 is formed to be larger than the hole 18 and is attached to the inner surface of the lid 12 by an appropriate method such as adhesion or welding. As a result, the hole 18 is completely closed.

The dialysis membrane 19 may be formed of a suitable material such as cellulose, cellulose acetate, polyacrylonitrile, polysulfone, polyether sulfone, polyaramid, etc., to have a predetermined thickness.

Next, the operation of the dialysis container 1 will be described. When performing dialysis of the liquid sample S in the dialysis container 1, first, as shown by a two-dot chain line in FIG.
Remove the lid 12 from the. Then, the dialysis membrane 19 attached to the lid 12 is also removed and the opening 13 is opened. Next, the sample S is injected into the container body 11 through the opening 13.

Next, as shown by the solid line in FIG.
Is attached to the container body 11. With this, the dialysis membrane 19 is fixed, and the opening 13 of the container body 11 and the hole 18 of the lid 12 are separated by the dialysis membrane 19. The sample S is now treated with the dialysis membrane 19
Then, the dialysis treatment can be performed. Dialysis container 1
When is immersed in a dialysis liquid, components capable of dialyzing the dialysis membrane 19 flow out through the holes 18 of the lid 12 to the outside.

In this dialysis container 1, the container body 1
Since there is no gap in the inside of 1 and the lid 12 that makes it difficult for the sample S to enter and be taken out, dialysis treatment can be performed even when the amount of the sample S is small.

Further, the dialysis membrane 19 is fixed only by attaching the lid 12 to the container body 11 by screw connection, whereby the opening 13 of the container body 11 and the hole 18 of the lid 12 are separated by the dialysis membrane 19, Since the dialysis treatment of the sample S in the main body 11 can be performed, the handling property is significantly improved and a large number of specimens can be easily treated. Further, since the structure can be simplified as compared with the conventional one, the cost can be reduced.

FIG. 2 shows a dialysis container 2 according to a second embodiment of the present invention. The dialysis container 2 includes a bottomed cylindrical container body 21 and a lid 2 for opening and closing an opening 23 of the container body 21.
2 is provided. A collar 24 is provided around the opening 23 of the container body 21. The lid 22 is movably connected to the collar 24 of the container body 21 by a hinge 25.

The lid 22 is made of an elastic material and has a flat plate portion 26.
And an annular protrusion 27 provided on the inner surface of the flat plate portion 26. The protrusion 27 is formed to have a diameter slightly larger than the opening 23 of the container body 21. Then, the projection 27 is applied to the opening 23 and pressed against the lid 22, so that the projection 27 is elastically contracted and fitted into the container body 21, and the elastic restoring force of the projection 27 generated at this time causes the lid 22 to move. Are locked to the container body 21.

The flat plate portion 26 of the lid 22 has a container body 2
A hole 28 having a predetermined size is provided at a position corresponding to the first opening 23. A dialysis membrane 29 is attached to the inside of the protrusion 27 so as to close the hole 28.

In this dialysis container 2 as well, as in the case of the dialysis container 1 described above, there is no gap that makes it difficult to take out the sample S inside the container body 21 and the lid 22, so that when the amount of the sample S is small. However, dialysis treatment can be performed.

Further, the dialysis membrane 29 is fixed only by pressing the lid 22 and attaching it to the container body 21, whereby the dialysis membrane 29 is fixed.
Since the opening 23 and the hole 28 are partitioned by the dialysis membrane 29 and the sample S can be dialyzed, the handling property is significantly improved. Furthermore, since the configuration can be simplified,
Cost reduction is possible.

FIG. 3 shows a dialysis container 3 according to a third embodiment of the present invention. The dialysis container 3 includes a bottomed cylindrical container body 31 and a cap-shaped lid 32 that opens and closes an opening 33 of the container body 31. Container body 31 and lid 32
And are detachably coupled with respective screws 34 and 35.

The screw 34 of the container body 31 is provided at a position separated from the opening 33 by a predetermined dimension, and a step portion 36 is provided between the opening 33 and the screw 34. The edge of the screw 35 of the lid 32 is chamfered 37. Also,
The lid 32 is provided with a hole 38 having a predetermined size at a position corresponding to the opening 33.

An O-ring 39 is placed on the edge of the container body 31 on the side of the opening 33, and the cap-shaped dialysis membrane 4 is placed on the O-ring 39.
0 is coated. That is, the O-ring 39 and the dialysis membrane 40 are arranged between the container body 31 and the lid 32.

The O-ring 39 and the dialysis membrane 40 are pressed by the lid 32 toward the container body 31 with a predetermined force, and the O-ring 39 is flattened. As a result, the gap between the container body 31 and the dialysis membrane 40 is sealed by the O-ring 39, and the opening 33 of the container body 31 and the hole 3 of the lid 32 are sealed.
8 and 8 are completely separated by the dialysis membrane 40.

Also in this dialysis container 3, the dialysis membrane 39 is fixed only by attaching the lid 32 to the container body 31.
As a result, the opening 33 and the hole 38 are completely partitioned, and the sample S in the container body 31 can be dialyzed by the dialysis membrane 40. Therefore, the handleability is improved, and the configuration can be simplified to reduce the cost. Further, since there is no gap between the container body 31 and the lid 32 that makes it difficult for the sample S to enter and be taken out, a small amount of sample S can be dialyzed.

Further, in this dialysis container 3, the lid 3
Since it is not necessary to attach the dialysis membrane 40 to 2,
Can be easily replaced. Therefore, for example, it is suitable for performing dialysis treatment using various dialysis membranes 40 made of different materials.

In the above embodiment, the lids 12 and 2 are
2, 32 are provided with holes 18, 28, 38, and these holes 18, 2
The dialysis membranes 19, 29, and 40 are used to block 8 and 38, but like the dialysis container 5 of the fourth embodiment shown in FIG.
A hole 53 may be provided in the bottom portion 52 of the container body 51, and the hole 53 may be closed by a dialysis membrane 54 attached to the inside or outside of the bottom portion 52 (the inside is shown in the figure). The lid 56 is
It is completely blocked. It is also possible to provide a hole (not shown) of a predetermined size in the side portion 55 of the container body 51 and close the hole with a dialysis membrane. The dialysis container 5 has a very simple structure, so that the cost can be significantly reduced.

[0031]

As described above, according to the dialysis container of the first invention, the dialysis membrane is fixed only by attaching the lid to the container body, and the opening of the container body and the hole of the lid corresponding to this opening are formed. Is partitioned by the dialysis membrane, and the sample in the container main body can be dialyzed by the dialysis membrane.

Therefore, the handling property is improved, a large number of specimens can be easily processed, and the structure can be simplified to reduce the cost. In addition, since there is no gap inside the container body and the lid that makes it difficult for the sample to enter and be taken out, dialysis treatment can be performed on a small amount of sample.

Further, according to the dialysis container of the second aspect of the present invention, a hole is provided at the bottom or side of the container body and the hole is closed by the dialysis membrane, so that the structure is very simple and the cost is greatly reduced. It becomes possible to plan.

[Brief description of drawings]

FIG. 1 is a sectional view showing a dialysis container of a first embodiment according to the present invention.

FIG. 2 is a sectional view showing a dialysis container according to a second embodiment of the present invention.

FIG. 3 is a sectional view showing a dialysis container of a third embodiment according to the present invention.

FIG. 4 is a sectional view showing a dialysis container of a fourth embodiment according to the present invention.

[Explanation of symbols]

 1, 2, 3, 5 Dialysis container 11, 21, 31, 51 Container body 12, 22, 32, 56 Lid 13, 23, 33 Opening 14, 15, 34, 35 Screw 18, 28, 38, 53 Hole 19 , 29, 40, 54 Dialysis membrane 27 Annular protrusion 39 O-ring 52 Bottom part 55 Side part S sample

Claims (6)

[Claims] 1. A bottomed cylindrical container main body having an opening through which a sample to be dialyzed can be put in and taken out, and a lid for opening and closing the opening, and a predetermined size is provided at a position corresponding to the opening of the lid. A hole is provided, a dialysis membrane is arranged between the container body and the lid, and when the lid is attached to the container body, the opening and the hole are partitioned by the dialysis membrane. Characteristic dialysis container. 2. A bottomed cylindrical container body having an opening through which a sample to be dialyzed can be taken in and out, and a lid for opening and closing the opening, the container body having a predetermined size at the bottom or side thereof. The dialysis container characterized in that the hole is provided and the hole is closed by a dialysis membrane. 3. The dialysis container according to claim 1, wherein the dialysis membrane is attached to an inner surface of the lid. 4. The lid according to claim 1, wherein the lid is attached to the container body by screw connection.
Alternatively, the dialysis container according to claim 3. 5. The lid is provided with an annular protrusion having elasticity, and the protrusion is fitted into the opening to attach the lid to the container body.
The dialysis container according to claim 2 or 3. 6. The dialysis container according to claim 1, claim 2, claim 3, claim 4, or claim 5, wherein the container body is a microtube.