JPH09266787A - New strain of bacillus thuringiensis and insecticide for diptera noxious insect - Google Patents
New strain of bacillus thuringiensis and insecticide for diptera noxious insectInfo
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- JPH09266787A JPH09266787A JP8103198A JP10319896A JPH09266787A JP H09266787 A JPH09266787 A JP H09266787A JP 8103198 A JP8103198 A JP 8103198A JP 10319896 A JP10319896 A JP 10319896A JP H09266787 A JPH09266787 A JP H09266787A
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- bacillus thuringiensis
- insecticide
- diptera
- new strain
- insecticidal activity
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Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、バチルス・チューリン
ゲンシス（Bacillus thuringiensis）分離株に関し、特
に、この菌による双翅目害虫の駆除に関する。FIELD OF THE INVENTION The present invention relates to a Bacillus thuringiensis isolate, and more particularly to the control of Diptera pests by this bacterium.
【０００２】[0002]
【従来の技術】化学殺虫剤に代わる微生物殺虫剤とし
て、最も広く利用されている種々のバチルス・チューリ
ンゲンシス ( Bacillus thuringiensis 、以下ＢＴと言
う) は、胞子形成期に結晶性毒素タンパクを形成し、こ
の毒素タンパクを摂食した害虫は、腸管破裂等ののち死
に至ることが知られている。2. Description of the Related Art Various Bacillus thuringiensis (hereinafter referred to as BT), which are most widely used as microbial insecticides replacing chemical insecticides, form crystalline toxin proteins during sporulation. It is known that pests that feed on this toxin protein die after rupture of the intestine and the like.
【０００３】ＢＴはその亜種の違いにより殺虫活性が異
なっており、例えば亜種クルスタキー、アイザワイは鱗
翅目、双翅目昆虫に毒性を示し(Dulmage,H.T.: J. Inve
rtebr. Pathol., 15, 232 (1970)) 、イスラエレンシ
ス、ダルムスタディエンシスは双翅目昆虫に(L. J. Gol
dberg : Mosq. News, 37, 355 (1977))(L.E.padua : J.
Invertebr. Pathol., 44, 12 (1984)) 、またテネブリ
オニスは鞘翅目の甲虫類にそれぞれ毒性を示すことが知
られている（特開昭６０−１５６３８２号公報）。The insecticidal activity of BT differs depending on its subspecies. For example, the subspecies Kurustaky and Aizawai show toxicity to Lepidoptera and Diptera insects (Dulmage, HT: J. Inve.
rtebr. Pathol., 15, 232 (1970)), Isla Ellensis and Darmstadiensis are dipteran insects (LJ Gol
dberg: Mosq. News, 37, 355 (1977)) (LEpadua: J.
Invertebr. Pathol., 44, 12 (1984)), and Tenebrionis is known to be toxic to beetles of the order Coleoptera (JP-A-60-156382).
【０００４】また、このような微生物殺虫剤は、化学殺
虫剤に比べその対象とする害虫の適用範囲が狭いため、
その適用範囲を広げる目的で、数種のＢＴを混合して使
用したり、あるいは、遺伝子工学的手法により適用害虫
の範囲を広げるなどの方法がとられている。Further, such a microbial pesticide has a narrower scope of application of the target pest than a chemical pesticide,
For the purpose of expanding the range of application, methods of mixing several kinds of BT and using them, or expanding the range of pests to be applied by genetic engineering techniques have been adopted.
【０００５】しかしながら、近年これらのＢＴを用いた
微生物殺虫剤に関して、鱗翅目昆虫のコナガにおいて抵
抗性を持った系統が確認されているように、抵抗性害虫
の出現が、他のＢＴ殺虫剤でも同様に生じることが予想
されている。マラリアを媒介する蚊等の双翅目害虫の幼
虫に対して現在用いられているイスラエレンシスについ
ても、最初に単離されてすでに２０年近くが経過してお
り、耐性を持った系統の出現は必至である。そこで双翅
目昆虫に毒性を持つ、イスラエレンシス以外の新規バチ
ルス・チューリンゲンシスが求められている。However, in recent years, regarding the microbial insecticides using these BTs, the appearance of resistant pests has been confirmed by other BT insecticides, as strains having resistance in the lepidopteran insect, diamondback moth, have been confirmed. It is expected to occur as well. Islaelensis, which is currently used for larvae of malaria-borne mosquitoes and other dipteran pests, has been isolated for nearly 20 years, and has emerged as a resistant strain. Is inevitable. Therefore, a novel Bacillus thuringiensis other than Islaelensis, which is toxic to Diptera insects, is required.
【０００６】[0006]
【発明が解決しようとする課題】特にハマダラカは、マ
ラリアの原因となるマラリア原虫を媒介する昆虫で、世
界的に問題になっている。一方、チカイエカは、都市型
環境下で冬期でも気温が高ければ吸血活動を示し、衛生
不快害虫として問題になっている。ハマダラカやチカイ
エカは、均衡がとれている生態系の中や人家の近くで発
生する害虫であるため、使用する殺虫剤は生態系の破壊
を最小限に抑え、人畜に対して無害である必要がある。
そこで、これらハマダラカ及びチカイエカに対し特異的
に毒性を示す新菌株の出現が望まれている。The anopheles mosquito, in particular, is an insect that carries a malaria parasite that causes malaria, and has become a worldwide problem. On the other hand, Chica mosquitoes show blood-sucking activity even in winter under urban environment even in winter, which is a problem as a sanitary unpleasant pest. Anopheles mosquitoes and chica mosquitoes are pests that occur in a balanced ecosystem and near homes, so the pesticides used should be minimally damaging to the ecosystem and harmless to humans and animals. is there.
Therefore, it is desired to develop new strains that are specifically toxic to Anopheles mosquito and Culex pipiens.
【０００７】本発明は上記の問題を解決するために、ハ
マダラカ(Anopheles sinensis)及びチカイエカ(Culex p
ipiens molestus)に対し特異的に毒性を示す新菌株を得
て、それらの害虫を防除することを目的とする。In order to solve the above problems, the present invention provides anopheles sinensis and Culex p.
The objective is to obtain new strains that are specifically toxic to ipiens molestus) and control those pests.
【０００８】[0008]
【課題を解決するための手段】本発明者は、上記目的を
達成するために鋭意研究の結果、ハマダラカ及びチカイ
エカに対し特異的に毒性を示す新菌株の分離に成功し、
本発明を完成するに至った。Means for Solving the Problems As a result of intensive research to achieve the above object, the present inventor succeeded in isolating a new strain that is specifically toxic to Anopheles mosquito and Culex pipiens,
The present invention has been completed.
【０００９】本発明者が『９２−ＫＵ−１３７−４』と
表示する新規分離株は、工業技術院生命工学工業技術研
究所に、受託番号『ＦＥＲＭ Ｐ−１５２３０』として
寄託されており、次に示すような特性を有する。The new isolate designated by the present inventor as "92-KU-137-4" has been deposited at the Institute of Biotechnology, Institute of Industrial Science with the deposit number "FERM P-15230". It has the characteristics shown in.
【００１０】（１）このバチルス・チューリンゲンシス
( Bacillus thuringiensis) 分離株９２−ＫＵ−１３７
−４は、熊本県の養蚕農家の塵埃より分離されたもので
ある。 （２）この新菌株は、普通寒天培地（ｐＨ７．６）上
で、大きな粗面性白色コロニーを形成する。 （３）この細菌はグラム陽性の桿菌で鞭毛を有する。 （４）バチルス・チューリンゲンシス(Bacillus thurin
giensis)の分類は、その鞭毛の血清型により行われる
が、９２−ＫＵ−１３７−４は、現在単離し分類されて
いるＨ血清型１〜４３の中には分類されない。この菌の
単離及び分類、並びにカ類に対する毒性は、今まで知ら
れておらず、本発明ではじめて明らかにしたものであ
る。(1) This Bacillus thuringiensis
(Bacillus thuringiensis) Isolate 92-KU-137
-4 was separated from the dust of sericulture farmers in Kumamoto Prefecture. (2) This new strain forms large rough white colonies on ordinary agar medium (pH 7.6). (3) This bacterium is a Gram-positive bacillus and has flagella. (4) Bacillus thurin
giensis) is classified by its flagella serotype, but 92-KU-137-4 is not classified among the currently isolated and classified H serotypes 1-43. The isolation and classification of this bacterium, and the toxicity to mosquitoes have not been known until now, and have been clarified for the first time in the present invention.
【００１１】かくして、本発明によれば、バチルス・チ
ューリンゲンシス新菌株９２−ＫＵ−１３７−４（生命
工学工業技術研究所受託番号ＦＥＲＭ Ｐ−１５２３
０）の培養物を有効成分とする、ハマダラカ及びチカイ
エカに対して殺虫活性を有する双翅目害虫用殺虫剤が得
られる。Thus, according to the present invention, a new Bacillus thuringiensis strain 92-KU-137-4 (Bioengineering and Industrial Technology Research Center Accession No. FERM P-1523)
An insecticide for Diptera pests having an insecticidal activity against Anopheles mosquito and Culex pipiens, which contains the culture of 0) as an active ingredient, can be obtained.
【００１２】分離株９２−ＫＵ−１３７−４の培養は、
標準的な培地を用いて行うことができる。例えば寒天培
地を用い、３０℃で培養した場合、３日から５日で胞子
を形成し、結晶性の毒素タンパクを生産する。有効成分
となる分離株９２−ＫＵ−１３７−４の胞子と結晶性毒
素タンパクは、寒天培地上からかき取ることにより回収
することができる。また本発明の新規分離株は、適当な
液体培地を用い培養することもでき、その場合には、遠
心分離もしくはろ過を行うことにより有効成分を回収す
ることができる。Cultivation of isolate 92-KU-137-4
It can be performed using a standard medium. For example, when cultured at 30 ° C. using an agar medium, spores are formed in 3 to 5 days and a crystalline toxin protein is produced. The spores and crystalline toxin protein of isolate 92-KU-137-4, which are the active ingredients, can be recovered by scraping them from the agar medium. Further, the novel isolate of the present invention can be cultured in an appropriate liquid medium, and in that case, the active ingredient can be recovered by performing centrifugation or filtration.
【００１３】本発明の新規分離株９２−ＫＵ−１３７−
４によって胞子形成期に形成される結晶性毒素タンパク
は、電気泳動測定（ＳＤＳ−ＰＡＧＥ）したときに、４
０キロダルトンおよび５０キロダルトン付近に強いライ
ンを有し、さらに、３０，７０，１３０キロダルトン付
近にも弱いラインを有するような分子量を有するもので
あることが見いだされている。この結晶性毒素タンパク
は、溶血活性を示さない。The novel isolate 92-KU-137- of the present invention
The crystalline toxin protein formed by 4 in the sporulation period was 4% when electrophoresed (SDS-PAGE).
It has been found to have a molecular weight such that it has strong lines near 0 and 50 kilodaltons and weak lines near 30, 70 and 130 kilodaltons. This crystalline toxin protein does not show hemolytic activity.
【００１４】本発明の双翅目害虫用殺虫剤は、回収した
分離株９２−ＫＵ−１３７−４、もしくはこの菌が生産
する結晶性毒素タンパクを水に懸濁するか、または適当
な公知の配合剤を加えることによって得た混合物として
提供され、これを対象害虫に摂食させることにより駆除
を行う。The insecticide for dipterous pests of the present invention is prepared by suspending the recovered isolate 92-KU-137-4 or the crystalline toxin protein produced by this bacterium in water, or by using a suitable known agent. It is provided as a mixture obtained by adding the compounding agent, and is exterminated by feeding the target pest.
【００１５】[0015]
〔バチルス・チューリンゲンシス分離株９２−ＫＵ−１
３７−４の単離〕熊本県養蚕農家の塵埃０．５ｇを滅菌
水４．５ｍｌを入れた試験管に秤取り、十分に懸濁させ
た。次にこの土壌懸濁液（１０-1希釈液）を７０℃の水
浴中で１０分間放置し熱処理を行った。次に更に希釈す
るため、前記の土壌懸濁液を０．５ｍｌ取り、滅菌水
４．５ｍｌに加え１０-2希釈液を調整した。同様の方法
で１０-5希釈液まで順次段階希釈液を調整した。[Bacillus thuringiensis isolate 92-KU-1
Isolation of 37-4] 0.5 g of dust from a sericulture farmer in Kumamoto Prefecture was weighed into a test tube containing 4.5 ml of sterilized water and sufficiently suspended. Next, this soil suspension (10 −1 diluted solution) was left in a water bath at 70 ° C. for 10 minutes for heat treatment. Then, for further dilution, 0.5 ml of the soil suspension was taken and added to 4.5 ml of sterilized water to prepare a 10 -2 diluted solution. By the same method, serially diluted solutions were sequentially prepared up to 10 -5 diluted solutions.
【００１６】次にこの希釈試料を普通寒天培地（肉エキ
ス１０ｇ、ポリペプトン１０ｇ、塩化ナトリウム２ｇ、
寒天２０ｇ、水１リットル、ｐＨ７．６）に０．１ｍｌ
塗抹し、２８℃で３日から５日間培養した。所定期間培
養後生育してきたコロニーを位相差顕微鏡下で観察し、
白色に観察される胞子と、黒色に観察される結晶タンパ
クが同一細胞内に認められたものを選択した。Next, the diluted sample was added to an ordinary agar medium (meat extract 10 g, polypeptone 10 g, sodium chloride 2 g,
Agar 20 g, water 1 liter, pH 7.6) 0.1 ml
It was smeared and cultured at 28 ° C. for 3 to 5 days. Observe the colonies that have grown after culturing for a predetermined period under a phase contrast microscope,
Those in which spores observed in white and crystal proteins observed in black were found in the same cell were selected.
【００１７】〔実施例２〕 〔バチルス・チューリンゲンシス分離株９２−ＫＵ−１
３７−４の血清型の同定〕Ｈ血清型の同定は、大庭らの
方法(Ohba,M. : J.Invertebr. Pathol. 32,303-309 (19
78))で行った。本発明の新規Bacillus thuringiensis分
離株９２−ＫＵ−１３７−４をＬ型試験管中の４ｍｌの
培地（肉エキス１０ｇ、ポリペプトン１０ｇ、塩化ナト
リウム２ｇ、水１リットル、ｐＨ７．６）に懸濁し、３
７℃で３〜４時間、静かに振とうした。３〜４時間後、
この懸濁液１滴を、スライドガラス上で、２０〜５０倍
に希釈したＨ血清（Ｎｏ．１〜４３）１滴と混合した。
３〜５分後に凝集反応を判定した結果、既知の全てのＨ
血清（Ｎｏ．１〜４３）とも反応せず、全く新規の分離
株であることを確認し、血清型４４，セロバーヒゴと命
名した。Example 2 Bacillus thuringiensis isolate 92-KU-1
Identification of Serotype of 37-4] H serotype was identified by the method of Ohba et al. (Ohba, M .: J. Invertebr. Pathol. 32, 303-309 (19
78)). The novel Bacillus thuringiensis isolate 92-KU-137-4 of the present invention was suspended in 4 ml of a medium (meat extract 10 g, polypeptone 10 g, sodium chloride 2 g, water 1 liter, pH 7.6) in an L-type test tube, and 3
Gently shaken for 3-4 hours at 7 ° C. After 3-4 hours,
1 drop of this suspension was mixed with 1 drop of H serum (No. 1-43) diluted 20 to 50 times on a slide glass.
As a result of determining the agglutination reaction after 3 to 5 minutes, all known H
It was confirmed that it was a completely new isolate that did not react with serum (No. 1-43), and was designated as serotype 44, serobarhigo.
【００１８】〔実施例３〕 〔バチルス・チューリンゲンシス分離株９２−ＫＵ−１
３７−４の双翅目昆虫に対する毒性試験〕バチルス・チ
ューリンゲンシス分離株９２−ＫＵ−１３７−４を普通
寒天培地で２８℃で５日間培養後集菌し、蒸留水で２、
３回洗浄した。ハマダラカ(Anopheles stephensi) 、チ
カイエカ(Culex pipiens molestus)、オオチョウバエ(T
elmatoscopus albipunctatus) に対しては、胞子濃度が
１０8 ／ｍｌとなるように培養物を蒸留水に懸濁し、こ
の中にそれぞれの昆虫の幼虫５匹を入れ、２５℃で３日
間試験した。カイコ(Bombyx mori) 、アメリカシロヒト
リ(Hyphantria cunea)に対しては、幼虫５〜１０匹に対
してそれぞれ、０．１〜０．３ｍｌの懸濁液（１ｍｇ／
ｍｌ）を摂食させ、３日間試験した。またイエバエ（Mu
suca domestica)については、幼虫１匹あたりが１０8
個の胞子を食餌するように、餌の中に培養物を混入し、
同様に幼虫５匹に対し２５℃で３日間試験した。なお結
晶性タンパクは、胞子形成の際、１細胞から１個、もし
くはそれ以上形成される。Example 3 Bacillus thuringiensis isolate 92-KU-1
37-4 Toxicity test against Diptera insects] Bacillus thuringiensis isolate 92-KU-137-4 was cultivated on ordinary agar medium at 28 ° C. for 5 days and then collected, and distilled water was added to 2,
Washed three times. Anopheles stephensi, Culex pipiens molestus, Flying fly (T
For Elmatoscopus albipunctatus), the culture was suspended in distilled water so that the spore concentration would be 10 8 / ml, and 5 larvae of each insect were placed in the culture and tested at 25 ° C for 3 days. For Bombyx mori and Hyphantria cunea, 0.1-0.3 ml suspension (1 mg / mg) for 5-10 larvae, respectively.
ml) was fed and tested for 3 days. See also housefly (Mu
For suca domestica), 10 8 per larva
Mixing the culture in the diet, like feeding individual spores,
Similarly, 5 larvae were tested at 25 ° C. for 3 days. It should be noted that one or more crystalline proteins are formed from one cell during sporulation.
【００１９】毒性試験の結果を表１に示す。また、強い
殺虫活性のあったハマダラカ、チカイエカに対しては様
々な濃度に希釈した培養物懸濁液に、幼虫２０頭を入
れ、２４時間後の死虫数を測定し、プロビット法により
ＬＣ５０を求めた。その結果ＬＣ５０値はハマダラカに
対しては６．３μｇ／ｍｌ、チカイエカに対しては８
４．４μｇ／ｍｌであった。本実施例より、バチルス・
チューリンゲンシス分離株９２−ＫＵ−１３７−４は、
今まで殺虫活性が知られていなかった双翅目昆虫のハマ
ダラカ、チカイエカに活性を有することが明らかとなっ
た。The results of the toxicity test are shown in Table 1. Also, for Anopheles mosquitoes and Culex pipiens that had strong insecticidal activity, 20 larvae were placed in a culture suspension diluted to various concentrations, and the number of dead larvae was measured after 24 hours, and LC50 was determined by the probit method. I asked. As a result, the LC50 value was 6.3 μg / ml for Anopheles mosquito and 8 for Chica mosquito.
It was 4.4 μg / ml. From this example, Bacillus
Thuringiensis isolate 92-KU-137-4
It has been clarified that it has activity against the dipteran insects Anopheles mosquito and Culex pipiens, whose insecticidal activity has not been known until now.
【００２０】[0020]
【表１】 [Table 1]
【００２１】〔実施例４〕 〔バチルス・チューリンゲンシス分離株９２−ＫＵ−１
３７−４の生産する結晶性毒素タンパクの精製〕Bacill
us thuringiensis分離株９２−ＫＵ−１３７−４を普通
寒天培地（肉エキス１０ｇ、ポリペプトン１０ｇ、塩化
ナトリウム２ｇ、寒天２０ｇ、水１リットル、ｐＨ７．
６）で２７℃、５日間培養後、集菌し、１Ｍ塩化ナトリ
ウム溶液で洗浄、遠心を３回行った。結晶性毒素タンパ
クの部分精製は、硫酸デキストラン−ポリエチレングリ
コール系による二相分離法(Goodman. N. S. : J. Bact.
94, 485 (1967))により行った。結晶性毒素タンパクを
含む下層を水で希釈後、遠心して集めた。さらに純粋に
なるまで精製を行うために、ショ糖密度勾配遠心法を行
った。[Example 4] [Bacillus thuringiensis isolate 92-KU-1
Purification of crystalline toxin protein produced by 37-4] Bacill
us thuringiensis isolate 92-KU-137-4 on normal agar medium (meat extract 10 g, polypeptone 10 g, sodium chloride 2 g, agar 20 g, water 1 liter, pH 7.
After culturing at 6) for 5 days at 27 ° C, the cells were collected, washed with a 1M sodium chloride solution, and centrifuged three times. The crystalline toxin protein was partially purified by a two-phase separation method using a dextran sulfate-polyethylene glycol system (Goodman. NS: J. Bact.
94, 485 (1967)). The lower layer containing the crystalline toxin protein was diluted with water and then collected by centrifugation. Sucrose density gradient centrifugation was performed to further purify the product.
【００２２】７０Ｗ／Ｖ％、８０Ｗ／Ｖ％、９０Ｗ／Ｖ
％のショ糖溶液を１０ｍｌ容遠心管に重層し、さらに上
部に部分精製した結晶性毒素タンパクを０．５ｍｌ重層
した。２２，０００ｒｐｍで２時間遠心を行い、７０Ｗ
／Ｖ％−８０Ｗ／Ｖ％界面に沈降する、純粋に精製され
た結晶性毒素タンパクを回収した。この結晶性毒素タン
パクをＳＤＳ−ＰＡＧＥにかけたところ、４０キロダル
トンおよび５０キロダルトン付近に強いラインを有し、
さらに、３０，７０，１３０キロダルトン付近に弱いラ
インが認められた。70 W / V%, 80 W / V%, 90 W / V
% Sucrose solution was layered on a 10 ml centrifuge tube, and 0.5 ml of partially purified crystalline toxin protein was layered on top. Centrifuge at 22,000 rpm for 2 hours, 70W
Purely purified crystalline toxin protein was recovered which precipitated at the / V% -80W / V% interface. When this crystalline toxin protein was subjected to SDS-PAGE, it had strong lines near 40 and 50 kilodaltons,
Furthermore, a weak line was recognized around 30, 70, and 130 kilodaltons.
【００２３】[0023]
【発明の効果】本発明のバチルス・チューリンゲンシス
新菌株９２−ＫＵ−１３７−４は、ハマダラカ及びチカ
イエカに特異的な殺虫活性を有しており、これら害虫の
駆除に有効である。INDUSTRIAL APPLICABILITY The Bacillus thuringiensis strain 92-KU-137-4 of the present invention has an insecticidal activity specific to Anopheles mosquito and Culex pipiens, and is effective in controlling these pests.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ１２Ｒ 1:07） (72)発明者 水城 英一 福岡県筑紫野市大字上古賀332−１ 福岡 県 工業技術センター内 (72)発明者 宮本 和久 茨城県つくば市大わし１−２ 農林水産省 蚕糸・昆虫農業技術研究所内 (72)発明者 大庭 道夫 福岡県福岡市東区箱崎６−10−１ 九州大 学 内─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification number Internal reference number FI technical display location C12R 1:07) (72) Inventor Eiichi Mizuki Fukuoka Prefecture Chikushino City, Japan 332-1 Kamikoga, Fukuoka Prefecture Fukuoka Prefecture Industrial Technology Center (72) Inventor Kazuhisa Miyamoto 1-2 Owashi, Tsukuba City, Ibaraki Prefecture Ministry of Agriculture, Forestry and Fisheries, Research Institute for Silkworm and Insect Agriculture (72) Inventor Michio Ohba 6-10-1, Hakozaki, Higashi-ku, Fukuoka City Kyushu Univ. On campus
Claims (2)
活性を有するバチルス・チューリンゲンシス新菌株９２
−ＫＵ−１３７−４（生命工学工業技術研究所受託番号
ＦＥＲＭ Ｐ−１５２３０）。1. A new Bacillus thuringiensis strain 92 having insecticidal activity against Anopheles mosquito and Culex pipiens.
-KU-137-4 (Contract No. FERM P-15230, Institute of Life Science and Technology).
２−ＫＵ−１３７−４（生命工学工業技術研究所受託番
号ＦＥＲＭ Ｐ−１５２３０）の培養物を有効成分とす
るハマダラカ及びチカイエカに対して殺虫活性を有する
双翅目害虫用殺虫剤。2. A new Bacillus thuringiensis strain 9
An insecticide for diptera pests having an insecticidal activity against Anopheles mosquito and Chica mosquito, which comprises a culture of 2-KU-137-4 (Contract No. FERM P-15230 of the Institute of Biotechnology and Industrial Technology) as an active ingredient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8103198A JPH09266787A (en) | 1996-03-29 | 1996-03-29 | New strain of bacillus thuringiensis and insecticide for diptera noxious insect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8103198A JPH09266787A (en) | 1996-03-29 | 1996-03-29 | New strain of bacillus thuringiensis and insecticide for diptera noxious insect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH09266787A true JPH09266787A (en) | 1997-10-14 |
Family
ID=14347831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8103198A Pending JPH09266787A (en) | 1996-03-29 | 1996-03-29 | New strain of bacillus thuringiensis and insecticide for diptera noxious insect |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH09266787A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012065582A (en) * | 2010-09-22 | 2012-04-05 | National Institute Of Agrobiological Sciences | Bt toxin resistance gene of lepidoptera insect origin, and using thereof |
-
1996
- 1996-03-29 JP JP8103198A patent/JPH09266787A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012065582A (en) * | 2010-09-22 | 2012-04-05 | National Institute Of Agrobiological Sciences | Bt toxin resistance gene of lepidoptera insect origin, and using thereof |
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