JPH09257795A - Slide plate washing solution and kit containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a washing soln. preventing the deformation or falling-off of antigen cell resulting from the drying of the surface of a slide plate in an indirect immunofluorescence or indirect enzyme antibody technique and a kit containing the same. SOLUTION: A slide plate 2 having cells or tissue containing an antigen bonded to an antibody to be detected fixed thereon and a labelled antibody labelled with a label substance and bonded to the above-mentioned antibody are used and the label bonded by the reaction of the above-mentioned antigen, the labelled antibody and the antibody to be measured in a specimen is detected on the slide plate by a microscope to detect the above-mentioned antibody. At this time, a humectant is added to a washing soln. used in order to remove the unreacted antibody and/or the labelled antibody from the slide plate 2.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a washing solution used for washing a slide plate such as a slide glass used in a fluorescent antibody method or an enzyme antibody method using a microscope, and a kit containing the washing solution. The present invention relates to a cleaning solution for preventing the drying of the product and the damage of the test substance derived therefrom. The cleaning solution and kit of the present invention can be suitably used particularly for an automated cleaning device.

[0002]

2. Description of the Related Art For clinical examination and research using a microscope,
There are fluorescent antibody methods, enzyme antibody methods, pathological histochemistry, etc., and many of these methods include a staining and washing step.

The fluorescent antibody method includes anti-nuclear antibody, anti-DNA antibody (Clysidia method), anti-gastric parietal cell antibody, anti-smooth muscle antibody,
Autoimmune disease tests that detect anti-mitochondrial antibodies, anti-neutrophil cytoplasmic antibodies, etc., EB virus, rubella virus antibody, herpes virus antibody (antigen), cytomegalovirus antibody, chlamydia trachomatis antibody (antigen), chlamydia sititasi It is a test method used for an infectious disease test for detecting an antibody (antigen), endotoxin, toxoplasma antibody, FTA-ABS, etc., and histological diagnosis of cancer.

This test method is classified into a direct method and an indirect method. In the direct method, the tissues, cells, etc. of the subject are fixed on a slide plate, and a fluorescently labeled antibody is reacted with the immunological staining. The image is viewed under a microscope. In addition, in the indirect method, an antigen such as a cell or a tissue is usually fixed on a slide plate in advance, an antibody against the antigen in a test sample is reacted, and then fluorescently labeled and bound to the second antibody. Is further reacted, and the presence of the target antibody in the test sample is detected by observing the immunologically stained image with a fluorescence microscope.

In the enzyme antibody method, instead of the fluorescently labeled antibody of the above-mentioned fluorescent antibody method, an antibody labeled with an enzyme such as peroxidase or alkaline phosphatase, or
It is a method of performing immunological detection by observing the color of a substrate developed by an enzymatic reaction with a light microscope using a biotin-labeled antibody to which an enzyme can indirectly bind via a biotin-avidin bond. Except for this point, the method is exactly the same as the fluorescent antibody method.

In the conventional indirect fluorescent antibody method and indirect enzyme antibody method using a microscope, test samples and reagents are dispensed onto a slide glass, and the slide glass is washed with physiological saline in a washing layer such as a beaker. Since the two types of operations of cleaning in step 1 are repeated alternately, automation of the inspection process was the most difficult field.

Taking the indirect fluorescent antibody method of antinuclear antibody as an example, a general inspection procedure is shown below. 1) The slide glass used has a plurality of inspection holes, the glass is exposed at that portion, and the other portion is water-repellent coated with Teflon or the like. A cancer cell HEp2 (having a nuclear antigen) is fixed to the exposed test hole of the glass. 2) A patient serum diluted with physiological saline or the like is placed on a slide glass and reacted with a specific antibody (antinuclear antibody) in the serum and an antigen on the slide glass. 3) Soak the slide glass in physiological saline in a beaker for washing. 4) Place the fluorescently labeled anti-immunoglobulin antibody in the test hole on the slide glass and react with the specific antibody bound to the antigen on the slide glass. 5) Soak the slide glass in physiological saline in a beaker for washing. 6) Place the aqueous glycerin solution (mounting medium) on a slide glass, cover it with a cover glass, and observe with a fluorescence microscope.

[0008] As described above, in the inspection process using the slide glass, the dispensing of the sample or the anti-immunoglobulin antibody on the slide glass and the washing of the slide glass in the beaker are repeated, which makes automation difficult. Most of the process is done manually. As a result, clinical examination using such a microscope has become the least automated field in which most other clinical examinations have been automated.

In response to such a situation, the present inventors have already made it possible to perform automation, which was difficult as described above, by mounting an upper member having a detachable through hole on the slide glass. He invented a device to do so and applied for a patent (Japanese Patent Application No. 6-247750).

[0010]

When the automatic washing mechanism as described above is applied to a slide glass on which cells are fixed,
It has been found that there are some inconveniences when using an aqueous cleaning solution that has been used for manual cleaning.

In conventional hand washing, physiological saline (usually about 0.8 to 0.9% NaCl aqueous solution) is used as the washing liquid.
Alternatively, a buffer solution such as phosphoric acid is added to this to adjust the pH to near neutral (for example, a so-called “PBS”) or the like is used. This is filled in a cleaning tank such as a beaker, the slide glass placed in a basket for cleaning is immersed, and then the basket and the slide glass are taken out. The slide glass is washed by repeating this operation.

On the other hand, in the cleaning using the automatic cleaning machine, after the cleaning liquid is injected from the nozzle, it is repeatedly sucked from the suction nozzle. In this suction process, the cleaning liquid is removed by the suction force of the decompression pump, etc., so a certain level of strong suction force is required to sufficiently remove the cleaning liquid, and after the cleaning liquid is exhausted, an air flow will be generated in the cleaning area. It is easy to cause the surface to dry. In the indirect fluorescent antibody method and the indirect enzyme antibody method, cells of animals and microorganisms are immobilized as an antigen on a slide glass, and these immobilized antigens have extremely weak properties against such type of drying. Therefore, when washing with an automatic washing machine using a conventional washing solution, deformation due to drying of the immobilized antigen (cell),
Damage and detachment from glass slides are observed. Therefore, in order to wash slide glasses with an automatic washing machine,
It turned out that it was necessary to have a mechanism to prevent the slide glass from drying during cleaning.

Further, in the slide glass used for such an inspection, as described above, the portion other than the inspection hole on the slide glass is usually coated with water repellent material such as Teflon. The water-repellent coating prevents the sample and the reagent from mixing between the inspection holes, and therefore the water-repellent property of this coating is extremely important for the inspection. In the conventional cleaning method, since the whole slide glass is immersed in the cleaning tank, when a surfactant is added to the cleaning liquid, this water repellency is rapidly lost, which causes a trouble in the steps after cleaning. Therefore, it has been practically impossible to add substances such as surfactants and high-concentration proteins that reduce the water repellency of the coating to the cleaning liquid for the conventional cleaning method.

In view of the above, the present invention has an object to improve the cleaning solution for slide glasses and to perform efficient cleaning by the above-described automatic cleaning mechanism for slide glasses. Furthermore, in the process leading to the present invention, even with manual washing with a conventional washing solution, it was found that the antigen cells were deformed and dropped due to the drying of the surface of the slide glass, and prevention of this may also be an object of the present invention. Is.

[0015]

[Means for Solving the Problems] The present inventors prevent the deformation, damage and detachment of the immobilized antigen due to drying during washing by including a moisturizer in the washing solution used in the fluorescent antibody method or the enzyme-linked immunosorbent antibody method. Therefore, it was found that the washing can be performed more efficiently. That is, the cleaning liquid containing the moisturizer is injected into the cleaning surface of the slide glass and suction is repeated. With this configuration, the cleaning surface can be prevented from being dried even when air flows through the strong suction force during suction. By doing so, it was found that the slide glass can be washed even by using the automatic washing machine, and the present invention has been completed.

That is, the present invention uses a slide plate on which cells or tissues containing an antigen that binds to an antibody to be detected are immobilized, and a labeled antibody that is labeled with a labeling substance and that binds to the antibody. When detecting the antibody by detecting the antigen, the labeled antibody and the label bound by the reaction with the antibody to be measured in the sample using a microscope on a slide plate, or the antigen to be detected A slide plate on which cells or tissues containing are immobilized, labeled with a labeling substance, and labeled with an antibody that binds to the antigen, or an antibody that binds to the antigen and a labeling substance, and A labeled antibody that binds is used, and the label bound by the reaction of the antibody and / or the labeled antibody with the antigen is used on a slide plate using a microscope. A wash solution for removing unreacted antibody and / or labeled antibody from a slide plate when detecting the antigen by detecting, and a slide plate wash solution containing a moisturizer. .

The present invention is also a kit for detecting cells or tissues containing an antigen by an antibody method using a microscope, which comprises a slide plate for immobilizing the cells or tissues and the washing solution. A kit for detecting an antibody by an antibody method using a microscope, comprising a slide plate on which cells or tissues containing an antigen that binds to the antibody are immobilized, and the above washing solution. Provide a kit.

Hereinafter, the present invention will be described in detail. First, the cleaning liquid of the present invention will be described. Suitable moisturizers to be added to the cleaning liquid of the present invention include polyalcohols such as glycerin, ethylene glycol, propylene glycol, diethylene glycol and polyethylene glycol;
Glucose, sucrose, lactose, galactose, maltose,
Sugars or sugar alcohols such as sorbitol, trehalose; serum albumin, serum globulin, gelatin,
Gelatin hydrolyzate, casein, casein hydrolyzate,
Proteins or protein hydrolysates such as ovalbumin; Tween20, Tween40, Tween80, Triton X-10
0, surfactants such as fatty acid sugar ester and fatty acid amino acid ester; water-soluble polymers such as dextran, Ficoll (Pharmacia), polyethyleneimine, polyvinylpyrrolidone and the like.

The cleaning liquid of the present invention is characterized by containing the above moisturizing agent, and the components other than the moisturizing agent are particularly different from those conventionally used in the fluorescent antibody method or the enzyme antibody method. There is no place. As a conventional cleaning solution, physiological saline or a solution containing a buffer solution component is used. The cleaning solution of the present invention can be based on these, but is not limited thereto. Specifically, 1 to 500 mM, preferably about 5 to 50 mM phosphate buffer, pH 4 to 10, preferably pH 6 to 8.
A cleaning solution such as As the buffer solution, other than phosphoric acid, for example, boric acid, glycine, barbital, trishydroxyaminomethane, HEPES, or any other commonly used buffer solution component can be used.
Also, those that give the cleaning solution an appropriate osmotic pressure, for example,
0.01-2.5%, preferably 0.8-0.9% N
It may contain aCl or the like.

The amount of the moisturizing agent added to the cleaning liquid is preferably 0.1% for polyalcohols such as glycerin.
1 to 10% by weight, more preferably 1 to 5% by weight,
For proteins such as bovine serum albumin, gelatin hydrolysates, casein hydrolysates, preferably 0.01-
10% by weight, more preferably 0.1 to 1% by weight, in the case of a surfactant such as Tween20, Triton X-100,
Preferably 0.001 to 0.5% by weight, more preferably 0.01 to 0.1% by weight, a water-soluble polymer,
For example, dextran is preferably 0.01 to 10% by weight, more preferably 0.1 to 5% by weight.

The surfactant has not only a moisturizing effect but also a cleaning effect. However, as described above, it has been practically difficult to apply the conventional cleaning method to the slide glass coated with water repellent material such as Teflon. However, when the invention is applied to an automatic washing method using an automatic washing machine that automatically injects and sucks the washing solution into the slide plate, it is necessary to add a surfactant to the washing solution. This is extremely useful because it can be performed and the cleaning efficiency can be improved.

In the present invention, the humectant may be used alone or as a mixture of two or more kinds. For example, when glycerin and Tween 20 (surfactant) are combined, a sufficient moisturizing effect and cleaning effect can be obtained.

In addition, in the indirect fluorescent antibody method and the indirect enzyme antibody method, after the dyeing step is completed, a very small amount of glycerin or polyethylene glycol aqueous solution is added as an encapsulating agent (for example, about 5 μl per inspection hole), and then a cover glass is covered. Is commonly practiced. When polyalcohols such as glycerin and polyethylene glycol are added to the cleaning solution, it is practically impossible to completely remove the cleaning solution from the support such as the slide glass after cleaning. It also serves as an encapsulant, which has the side effect of saving the labor of dispensing the encapsulant.

Next, the kit of the present invention will be described.
A first kit of the present invention is a kit for detecting cells or tissues containing an antigen by an antibody method using a microscope, comprising a slide plate for immobilizing the cells or tissues, and the above washing solution. And, including.

The above kit is used to detect cells or tissues containing an antigen by a direct method such as a direct fluorescent antibody method or a direct enzyme antibody method. When the kit of the present invention is applied to a method using one type of antibody (labeled antibody), a labeled antibody that is labeled with a labeling substance and that binds to the antigen (labeled antibody) is used. When applied to the double antibody method using two types of antibodies (primary antibody and labeled secondary antibody), the antibody that binds to the antigen (primary antibody) and a labeling substance, and A labeled antibody (labeled secondary antibody) that binds to the antibody is used. These labeled antibody, or primary antibody and labeled secondary antibody may be prepared separately from the kit, or may be included in the kit.

In the method using one kind of antibody, cells or tissues as a sample are immobilized on a slide plate, a labeled antibody is added thereto, and the labeled antibody is bound to an antigen contained in the cells or tissues on the slide plate. Then, the labeled antibody not bound to the antigen is removed by washing with a washing solution, and then the label is detected. In the double antibody method, a primary antibody is added to a slide plate on which cells or tissues as a sample are immobilized to bind the antibody to the antigen, and the labeled antibody not bound to the antigen is washed with a washing solution. The labeled secondary antibody is added to the primary antibody, the labeled secondary antibody is bound to the primary antibody, and the labeled secondary antibody not bound to the primary antibody is washed and removed to detect the label. .

The second kit of the present invention is a kit for detecting an antibody by an antibody method using a microscope, which comprises a slide on which cells or tissues containing an antigen that binds to the antibody are immobilized. Include a plate and 'the above washing solution.

The above kit is used to detect an antibody by an indirect method such as an indirect fluorescent antibody method or an indirect enzyme antibody method. That is, a sample is added to a slide plate on which cells or tissues containing an antigen are immobilized to allow the antibody in the sample to bind to the antigen, and the antibody not bound to the antigen is removed by washing with a washing solution, and then labeled. A labeled antibody that is labeled with a substance and that binds to the antibody is added to bind the labeled antibody to the antibody to be detected, and the unbound labeled secondary antibody is washed and removed, and then the label is detected. To do. The labeled antibody may be prepared separately from the kit or may be included in the kit.

The washing solution and kit of the present invention are the same as the washing solution and kit used in the usual fluorescent antibody method and enzyme antibody method except that they contain a moisturizer as the washing solution, and the components and elements that are usually used are used. Can be applied. For example, in addition to the above-mentioned elements, a buffer solution for diluting a sample, a color-developing substrate for an enzyme when used in the enzyme-antibody method may be contained. Further, a commonly used method can be adopted to fix the cells or tissues to the slide plate.

Although some of the components of the kit may be sold separately, they are used in combination for substantial inspection. Examples of the slide plate include a slide glass and an equivalent product other than glass, for example, a plastic plate such as polystyrene. As the slide glass, a slide glass having a plurality of inspection holes and other portions coated with Teflon is preferably used, but such a structure is not always necessary.

As the test substance, cells or tissues containing antigens or antibodies, for example, cells such as cancer cells and virus-infected cells, antinuclear antibodies, anti-DNA antibodies (Crysidia method), anti-gastric parietal cell antibodies, anti-smooth muscles Antibody, anti-mitochondrial antibody, anti-neutrophil cytoplasmic antibody, anti-EB virus antibody, rubella virus antibody, herpes virus antibody (or antigen),
Cytomegalovirus antibody, Chlamydia trachomatis antibody (or antigen), Chlamydia sittasis antibody (or antigen), endotoxin, toxoplasma antibody, FTA
-ABS.

When the test substance is a cell or tissue,
Usually, cells or tissues are collected from patients,
When the test substance is an antibody, the cell or tissue may be a cell containing an antigen to which the antibody to be detected binds, taken from a human or other animal, and a cultured cell containing the antigen. Or virus-infected cells or the like. For example, a tissue collected from the human stomach wall can be used for detecting the anti-gastric parietal cell antibody, and a cultured cell infected with a herpes virus can be used for detecting the herpesvirus antibody.

The labeled antibody or primary antibody in the first kit is an antibody that specifically binds to an antigen as a test substance, and may be a monoclonal antibody or a polyclonal antibody. The labeled secondary antibody in the first kit and the labeled antibody in the second kit are antibodies that bind to the immunoglobulin of the animal from which the primary antibody or the antibody that is the test substance is derived, and Those prepared in animals (for example, commonly used animals such as goats, rabbits, mice and rats) can be used.

As the labeling substance, a labeling substance used in a usual immunoassay can be used. Such labeling substances include enzymes that catalyze color development reactions, fluorescent substances,
Examples include radioisotopes and biotin, but for detection under a microscope, enzymes and fluorescent substances are preferable. Examples of the enzyme include peroxidase and alkaline phosphatase, and examples of the fluorescent substance include FITC (fluoroscein isothiocyanate), rhodamine, phycoerythrin, and phycocyanin. Further, diaminobenzidine and the like can be used as a substrate for the enzyme. When biotin is used as the labeling substance, detection can be carried out by further binding an avidin-bound enzyme or a fluorescent substance. To label the antibody with these labeling substances, a method usually used in the enzyme antibody method can be adopted.

When the labeling substance is an enzyme, the detection of the label is carried out by adding a substrate for the enzyme to the support and reacting the support to develop the resulting color. When the labeling substance is a fluorescent substance, the label can be detected by irradiating the slide plate with excitation light and detecting the generated fluorescence.

By using the washing solution or kit of the present invention, even if the washing solution is removed from the slide plate in the washing step, it is possible to prevent deformation of cells and tissues due to drying, damage and detachment from the slide plate.

[0037]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be specifically described. <1> Cleaning Liquid First, the form of the cleaning liquid will be illustrated. (1) Washing solution containing glycerin 10 mM sodium phosphate buffer (pH 7.5), 15
0 mM NaCl 5% glycerin

(2) Washing liquid containing a surfactant (Tween 20) 10 mM sodium phosphate buffer (pH 7.5), 15
0 mM NaCl 0.01% Tween 20

(3) Washing solution containing glycerin and Tween 20 10 mM sodium phosphate buffer (pH 7.5), 15
0 mM NaCl 5% glycerin 0.01% Tween20

(4) Washing solution containing bovine serum albumin 10 mM sodium phosphate buffer (pH 7.5), 15
0 mM NaCl 0.5% bovine serum albumin

(5) Washing Solution Containing Water-Soluble Polymer (Dextran) 10 mM Sodium Phosphate Buffer (pH 7.5), 15
0 mM NaCl 0.5% dextran

<2> Antinuclear Antibody Detection Kit Next, as a form of the kit of the present invention, an antinuclear antibody detection kit will be exemplified. The kit consists of the following elements.

A slide glass (Fig. 1) on which cancer cells Hep2 (having a nuclear antigen) is fixed (the slide glass has a plurality of inspection holes, the glass is exposed at that portion, and the other portion is made of Teflon or the like. Water-repellent coating and cancer cells Hep2 are fixed in the test holes of the previous term.) Washing solution (10 mM sodium phosphate buffer (pH 7.
5), 150 mM NaCl, 5% glycerin, 0.0
1% Tween20) Saline solution Rabbit anti-human immunoglobulin antibody solution labeled with fluorescent dye

<3> Example of detection of antinuclear antibody using the kit of the present invention (1) A plurality of (2 × 8) inspection holes 1 are provided, and glass is exposed at that portion, and other portions Prepares the cancer cells Hep2 fixed in the inspection holes of the slide glass 2 which is water-repellent coated with Teflon or the like. The cells are fixed, for example, by treating the slide glass to which the cells are adhered with acetone.

(2) A box-shaped lower member 3 capable of accommodating 6 slide glasses side by side is provided with a flat plate-shaped seal 4 having a size substantially equal to the inner size of the lower member 3, and the slide glasses 2 described above are placed thereon. Put on. A protrusion 5 is provided on the inside of the long side of the lower member 3 for fixing the slide glass. On top of that, a plurality of (12
An upper member 7 having (x8) through holes 6 and having a size substantially equal to the inner size of the lower member 3 is inserted into the lower member 3 and pressed onto the slide glass 2 to form an inspection cylinder (Fig. 1). ).

The lower member, the seal, and the upper member are
Although not essential for the detection of antigens and antibodies using the kit of the present invention, using them simplifies the operation. In particular, these members are useful for automating the injection and washing of reagents.

(3) Patient serum diluted with physiological saline or the like is dispensed into an inspection cylinder by an automatic dispenser and reacted with the specific antibody (antinuclear antibody) in the serum and the antigen on the slide glass 2. Let (4) Using an automatic washing machine that automatically injects and aspirates the cleaning liquid, the injection and aspiration of the cleaning liquid into the inspection cylinder are repeated twice or more. Thus, the antibody (immunoglobulin) that has not reacted with the antigen on the slide glass 2 is removed.

(5) A rabbit anti-human immunoglobulin antibody labeled with a fluorescent dye is dispensed into the test hole by an automatic dispenser and reacted with the specific antibody bound to the antigen on the slide glass 2.

(6) Using an automatic washing machine, injecting and aspirating the washing liquid into the inspection cylinder is repeated twice or more. In this way, the fluorescence-labeled anti-immunoglobulin not bound to the antinuclear antibody on the slide glass 2 is removed.

(7) The upper member 7 is removed from the slide glass 2, the aqueous glycerin solution is placed on the slide glass, covered with a cover glass, and observed with a fluorescence microscope.

[0051]

EXAMPLES The present invention will be described more specifically below with reference to examples. This example relates to the indirect fluorescent antibody method of the antinuclear antibody test. <Preparation of Reagents> (1) Cell-fixed slide glass Human cancer cells HEp2 were cultured to prepare HEp2 cell suspension (in MEM medium). The cell suspension was placed in the inspection hole 1 on the slide glass 2, the cells were adhered to the surface of the slide glass, and treated with acetone to fix the cells on the slide glass. The slide glass 2 is colored and water-repellent using a printing ink with Teflon as a binder, except for the inspection hole 1, and when the test sample or reagent is dispensed, the liquid protrudes from the inspection hole. To prevent it from being mixed with other samples.

(2) Labeled antibody solution A rabbit anti-human gamma globulin antibody was labeled with FITC and diluted with phosphate buffered saline (PBS) containing 1% bovine serum albumin.

(3) Washing solution 10 mM sodium phosphate buffer (pH 7.5), 15
0 mM NaCl, 5% glycerin, 0.01% Twe
A washing solution was prepared so as to be en20.

<Detection of antinuclear antibody> Serum samples from patients with collagen disease were diluted 20 times with PBS. Then, the cell-fixing slide glass 2 is added to the item <3> of the embodiment.
The same lower member 3, seal 4 and upper member 7 as in (2) are used, and the seal 4, slide glass 2,
The upper member 7 was placed in order and crimped to form a test tube (FIG. 1), and 50 μl of the diluted sample was dispensed into the test tube and left at room temperature for 30 minutes.

After injecting the above-mentioned cleaning liquid into the inspection cylinder using an automatic cleaning machine having a cleaning liquid injection mechanism and a suction mechanism,
The cleaning liquid was aspirated from the inspection cylinder. This operation was repeated five times. The volume of one injection was 300 μl. At this time, as a control, the moisturizer components (glycerin and T
The washing liquid except for the ween 20) was used.

After washing, the labeled antibody solution is placed in a test tube at 50 μm.
The solution was dispensed in increments of 1 and left at room temperature for 30 minutes, and then the same washing operation as above was repeated 5 times. Next, the upper member 7 was removed from the slide glass 2, and the slide glass 2 was covered with a cover glass. Since the above-mentioned cleaning liquid contains glycerin, if there is a residual cleaning liquid, no encapsulant is required. When a cleaning solution containing no moisturizing agent was used as a control, 10 μl of 10% glycerin was added as an encapsulating agent per test hole.

The specimen on the slide glass prepared as described above was observed for fluorescence with a fluorescence microscope. Schematic diagrams of the obtained fluorescence images are shown in FIGS. 2 and 3. As shown in this figure, when the washing solution of the present invention was used, undamaged cells were observed (FIG. 2), and when the conventional washing solution was used, cell damage and detachment were observed (FIG. 2). 3).

[0058]

EFFECT OF THE INVENTION By using the cleaning solution of the present invention, the slide glass can be efficiently washed in the antibody method such as the fluorescent antibody method and the enzyme antibody method.

[Brief description of drawings]

FIG. 1 is an external view (perspective view) showing a structure of an inspection apparatus according to an embodiment.

FIG. 2 is a conceptual diagram of a fluorescence image when washed with an automatic washing machine using the washing liquid of the present invention.

FIG. 3 is a conceptual diagram of a fluorescence image when washed with an automatic washing machine using a control washing solution.

[Explanation of symbols]

 1. Inspection hole 2. Slide glass 3. Lower member 4. Seal 5. Protrusion 6. Through hole 7. Upper member

Claims (9)

[Claims] 1. An antigen using a slide plate on which cells or tissues containing an antigen that binds to an antibody to be detected are immobilized, and a labeled antibody that is labeled with a labeling substance and that binds to the antibody. When detecting the antibody by detecting the labeled antibody and the label bound by the reaction with the antibody to be measured in the sample using a microscope on a slide plate, or cells containing the antigen to be detected. Or a slide plate on which a tissue is immobilized, and a labeled antibody that is labeled with a labeling substance and that binds to the antigen, or an antibody that binds to the antigen and a labeling substance, and a label that binds to the antibody And detecting the label bound by the reaction of the antibody and / or the labeled antibody with the antigen using a microscope on a slide plate using a microscope. Upon detecting the antigen I, a cleaning solution used to remove antibody and / or an unreacted labeled antibody from the slide plate, the slide plate cleaning solution, characterized in that it contains a humectant. 2. The cleaning liquid according to claim 1, wherein the humectant is selected from polyalcohols. 3. The cleaning liquid according to claim 2, wherein the humectant is selected from glycerin, ethylene glycol, propylene glycol, diethylene glycol and polyethylene glycol. 4. The moisturizing agent is used in an amount of 0.
The cleaning solution according to claim 1, which comprises 01% by weight or more of protein. 5. The cleaning solution contains 0.
The cleaning liquid according to any one of claims 1 to 4, which contains 001% by weight or more of a surfactant. 6. The cleaning liquid according to any one of claims 1 to 5, wherein the labeling substance is an enzyme or a fluorescent substance. 7. The cleaning liquid according to claim 1, wherein the cleaning is performed by an automatic cleaning machine that automatically injects and sucks the cleaning liquid into and from the slide plate. 8. A kit for detecting cells or tissues containing an antigen by an antibody method using a microscope, comprising a slide plate for immobilizing the cells or tissues,
A kit comprising the cleaning solution according to claim 1. 9. A kit for detecting an antibody by an antibody method using a microscope, comprising: a slide plate on which cells or tissues containing an antigen that binds to the antibody are immobilized; A kit containing the washing solution according to any one of claims.