JPH09248177A - Production of dried bacterium cell body - Google Patents
Production of dried bacterium cell bodyInfo
- Publication number
- JPH09248177A JPH09248177A JP8061273A JP6127396A JPH09248177A JP H09248177 A JPH09248177 A JP H09248177A JP 8061273 A JP8061273 A JP 8061273A JP 6127396 A JP6127396 A JP 6127396A JP H09248177 A JPH09248177 A JP H09248177A
- Authority
- JP
- Japan
- Prior art keywords
- dried
- bacterium cell
- freezing
- starter
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 10
- 241000894006 Bacteria Species 0.000 title abstract description 37
- 210000005056 cell body Anatomy 0.000 title 1
- 238000007710 freezing Methods 0.000 claims abstract description 23
- 230000008014 freezing Effects 0.000 claims abstract description 23
- 229910052751 metal Inorganic materials 0.000 claims abstract description 14
- 239000002184 metal Substances 0.000 claims abstract description 14
- 230000000813 microbial effect Effects 0.000 claims description 50
- 238000004113 cell culture Methods 0.000 claims description 23
- 238000001291 vacuum drying Methods 0.000 claims description 17
- 239000007858 starting material Substances 0.000 abstract description 27
- 238000001035 drying Methods 0.000 abstract description 9
- 239000002245 particle Substances 0.000 abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 241001608472 Bifidobacterium longum Species 0.000 abstract description 4
- 229940009291 bifidobacterium longum Drugs 0.000 abstract description 4
- 235000021107 fermented food Nutrition 0.000 abstract description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 50
- 235000014655 lactic acid Nutrition 0.000 description 25
- 239000004310 lactic acid Substances 0.000 description 25
- 230000000052 comparative effect Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 235000020183 skimmed milk Nutrition 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000002612 dispersion medium Substances 0.000 description 5
- 230000003472 neutralizing effect Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000010378 sodium ascorbate Nutrition 0.000 description 2
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 2
- 229960005055 sodium ascorbate Drugs 0.000 description 2
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 description 2
- 238000000859 sublimation Methods 0.000 description 2
- 230000008022 sublimation Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 235000015140 cultured milk Nutrition 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、乾燥微生物菌体を
製造する方法に関する。この乾燥微生物菌体は、発酵食
品を製造する際のスターター等として有用である。TECHNICAL FIELD The present invention relates to a method for producing dried microbial cells. This dried microbial cell is useful as a starter or the like when producing a fermented food.
【０００２】[0002]
【従来の技術】乾燥微生物菌体の代表例である発酵乳等
の製造に使用する乾燥乳酸菌スターターは、従来より、
脱脂乳等の培地で培養した乳酸菌培養物をバットに入れ
て冷凍庫内で凍結した後、真空乾燥することにより製造
されている。しかし、このような方法で乾燥乳酸菌スタ
ーターを製造すると、冷凍庫内に置かれたバット中の乳
酸菌培養物は徐々に凍結するので、凍結に長時間を要す
る。また、バット中の乳酸菌培養物は、表面が凍結して
いても中心部が完全に凍結していないことがあり、この
ような状態で真空乾燥を開始すると乾燥途中で凍結させ
た乳酸菌培養物が溶解することがある。そこで、乳酸菌
培養物を完全に凍結させることを目的として、凍結後さ
らに、凍結温度で通常５時間以上保持している現状にあ
る。このことは、乳酸菌培養物を凍結する際のコスト高
を引き起こしている。さらに、バット中で徐々に乳酸菌
培養物を凍結すると、凍結途中で乳酸菌培養物内での成
分移動が起こり、乾燥後の溶解性が悪くなる原因とな
る。そこで、乳酸菌培養物に中和剤や分散媒を添加する
ことになるが、中和剤や分散媒の添加は乳酸菌培養物の
体積をさらに増加させることから、乾燥効率を低下させ
る原因となる。2. Description of the Related Art A dry lactic acid bacterium starter used for producing fermented milk, which is a typical example of dry microbial cells, has been
It is manufactured by placing a lactic acid bacterium culture, which has been cultivated in a medium such as skim milk, in a vat, freezing it in a freezer, and then vacuum drying. However, when the dry lactic acid bacterium starter is manufactured by such a method, the lactic acid bacterium culture in the vat placed in the freezer is gradually frozen, and thus it takes a long time to freeze. Further, the lactic acid bacterium culture in the vat may not be completely frozen in the center even if the surface is frozen, and when vacuum drying is started in such a state, the lactic acid bacterium culture frozen during the drying is May dissolve. Therefore, for the purpose of completely freezing the lactic acid bacterium culture, after freezing, the freezing temperature is usually maintained for 5 hours or more. This causes high costs in freezing the lactic acid bacterium culture. Further, when the lactic acid bacterium culture is gradually frozen in the vat, the components move within the lactic acid bacterium culture during freezing, which causes deterioration of the solubility after drying. Therefore, a neutralizing agent or a dispersion medium is added to the lactic acid bacterium culture, but the addition of the neutralizing agent or the dispersion medium further increases the volume of the lactic acid bacterium culture, which causes a decrease in drying efficiency.
【０００３】さらに、このバット中で凍結した乳酸菌培
養物は、側面及び底面がバットで塞がれた状態となって
いることから、真空乾燥するに際し、凍結した乳酸菌培
養物から水分が昇華するのは上面のみとなり乾燥効率が
悪い。また、凍結した乳酸菌培養物の側面や底面のバッ
トに塞がれている部分の水分が昇華するのは、既に乾燥
した部分を通してであり、水分昇華の妨げとなると共
に、通過する水分が既に乾燥している部分に損傷を与え
ることにもなる。Further, since the lactic acid bacterium culture frozen in the vat has the side surface and the bottom face closed by the vat, water is sublimated from the frozen lactic acid bacterium culture during vacuum drying. Is only on the top surface and the drying efficiency is poor. In addition, the sublimation of water in the part covered by the vat on the side or bottom of the frozen lactic acid bacterium culture is through the already dried part, which hinders the sublimation of water and the water passing through is already dried. It will also damage the part that is doing.
【０００４】なお、乾燥が完了した乳酸菌培養物は、一
般に粉砕してから乳酸菌スターターとして使用されるこ
とが多く、凍結及び真空乾燥の設備とは別に粉砕の設備
を必要とする。しかも、粉砕に際しては、飛散による損
失も多く、雑菌汚染の危険性も高いという問題があっ
た。The dried lactic acid bacterium culture is generally used as a lactic acid bacterium starter after crushing, and a crushing facility is required in addition to the freeze and vacuum drying facilities. In addition, there is a problem that when crushing, there are many losses due to scattering and there is a high risk of contamination by various bacteria.
【０００５】一方、上記したような乾燥乳酸菌スタータ
ーの製造法の問題点を改善する目的で、乳酸菌培養物を
液体窒素中に滴下して急速凍結させた後、真空乾燥する
ことにより、ペレット状乾燥乳酸菌スターターを製造す
る方法が提案されている。この方法では、液体窒素中で
瞬間的に乳酸菌培養物を凍結するので氷晶が小さく、ま
た、乳酸菌培養物中の成分の分布も殆ど変化しないこと
から、凍結による障害や変質という問題を生じないとい
う特徴がある。しかし、この方法を実施するに当たって
は液体窒素を大量に使用する必要があり、安全性やコス
トの面で問題があった。On the other hand, for the purpose of improving the above-mentioned problems in the method for producing a dried lactic acid bacterium starter, a lactic acid bacterium culture is dropped into liquid nitrogen for rapid freezing, and then vacuum-dried to dry pellets. A method for producing a lactic acid bacterium starter has been proposed. In this method, since the lactic acid bacterium culture is instantly frozen in liquid nitrogen, the ice crystals are small, and the distribution of the components in the lactic acid bacterium culture hardly changes, so there is no problem of damage or alteration due to freezing. There is a feature called. However, in carrying out this method, it was necessary to use a large amount of liquid nitrogen, and there was a problem in terms of safety and cost.
【０００６】[0006]
【発明が解決しようとする課題】本発明者等は、上述し
た乾燥微生物菌体の製造における問題点を解決し、安全
で、低コストの乾燥微生物菌体製造法を提供するべく、
鋭意研究を進めていたところ、凍結温度以下に冷却した
金属板上に微生物菌体培養物の小滴を滴下して急速凍結
させた後、真空乾燥することにより、真空乾燥前の凍結
保持時間を短くすることができると共に真空乾燥の所要
時間も短くすることができ、また、真空乾燥した乾燥微
生物菌体を粉砕する必要もなく、さらに、中和剤や分散
媒等を添加しなくても良好な溶解性を有する乾燥微生物
菌体を得ることができることを見出し、本発明を完成す
るに至った。したがって、本発明は、凍結温度以下に冷
却した金属板上に微生物菌体培養物の小滴を滴下して急
速凍結させた後、真空乾燥することを特徴とする乾燥微
生物菌体の製造法を提供することを課題とする。DISCLOSURE OF THE INVENTION The present inventors have solved the above-mentioned problems in the production of dried microbial cells, and provided a safe and low-cost method for producing dried microbial cells.
As a result of diligent research, a small drop of the microbial cell culture was dripped onto a metal plate cooled below the freezing temperature for rapid freezing, followed by vacuum drying, thus preserving the freeze holding time before vacuum drying. Not only can it be shortened, the time required for vacuum drying can also be shortened, there is no need to pulverize the vacuum-dried dry microbial cells, and it is also possible to add no neutralizing agent or dispersion medium. It was found that a dry microbial cell having high solubility can be obtained, and the present invention has been completed. Therefore, the present invention provides a method for producing a dried microbial cell, which comprises rapidly dropping a droplet of a microbial cell culture on a metal plate cooled to a freezing temperature or lower to rapidly freeze it, and then vacuum drying. The challenge is to provide.
【０００７】[0007]
【課題を解決するための手段】本発明では、凍結温度以
下に冷却した金属板上に微生物菌体培養物の小滴を滴下
して急速凍結させた後、真空乾燥して乾燥菌体を得る。Means for Solving the Problems In the present invention, small cells of a microbial cell culture are dripped onto a metal plate cooled to a freezing temperature or below for rapid freezing, and then vacuum dried to obtain dried microbial cells. .
【０００８】本発明で、凍結温度以下に冷却した金属板
上に滴下する微生物菌体培養物は、通常行われている培
養方法に従って各微生物を培養し、得ることができる。
そして、この微生物菌体培養物を凍結温度以下に冷却し
た金属板上に滴下することにより、数秒以内で液滴の芯
まで急速凍結することができるので、バット中で微生物
菌体培養物を凍結する際に要した時間を大幅に短縮する
ことができる。In the present invention, the microbial cell culture that is dripped onto a metal plate cooled to a freezing temperature or lower can be obtained by culturing each microorganism according to a commonly used culturing method.
Then, by dropping this microbial cell culture onto a metal plate cooled below the freezing temperature, it is possible to quickly freeze to the core of the droplet within a few seconds, so freeze the microbial cell culture in the vat. It is possible to significantly reduce the time required for doing.
【０００９】次に、この微生物菌体培養物の凍結粒を回
収し、真空乾燥することにより、乾燥微生物菌体を得る
ことができる。特に、微生物菌体培養物の凍結粒を網カ
ゴ等に回収し、その状態で真空乾燥することで、真空乾
燥時間を大幅に短縮することができる。Next, the frozen particles of this culture of microbial cells are collected and vacuum dried to obtain dried microbial cells. In particular, the vacuum drying time can be significantly shortened by collecting frozen particles of the microbial cell culture in a net basket or the like and vacuum drying in that state.
【００１０】このようにして、従来のような真空乾燥後
の乾燥微生物菌体を粉砕する工程を必要とせずに、粒状
の乾燥微生物菌体を得ることができる。そして、この粒
状の乾燥微生物菌体は、成分分布が殆ど変わることなく
急速冷却されていることから溶解性に優れており、ま
た、分散媒や中和剤を添加する必要がないので不溶性凝
縮物等の残渣も生じない。したがって、直接、この粒状
の乾燥微生物菌体を原料に添加して使用することもでき
る。In this way, granular dry microbial cells can be obtained without the need for the conventional step of pulverizing the dried microbial cells after vacuum drying. This granular dry microbial cell has excellent solubility because it is rapidly cooled with almost no change in the component distribution, and since it is not necessary to add a dispersion medium or a neutralizing agent, the insoluble condensate No residue is generated. Therefore, the granular dry microbial cells can be directly added to the raw material for use.
【００１１】[0011]
【発明の実施の形態】本発明では、凍結温度以下に冷却
した金属板上に通常の方法で培養した微生物菌体培養物
を滴下して急速凍結させる。本発明において使用する金
属板については特に制限はないが、食品用の乾燥微生物
菌体を製造する場合は、ステンレス板を使用することが
好ましい。そして、このような金属板を冷媒等で凍結温
度以下に冷却する。BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, a microbial cell culture that has been cultivated by an ordinary method is dropped onto a metal plate cooled to a freezing temperature or below and rapidly frozen. The metal plate used in the present invention is not particularly limited, but it is preferable to use a stainless plate when producing dry microbial cells for food. Then, such a metal plate is cooled to a freezing temperature or lower with a refrigerant or the like.
【００１２】また、微生物菌体培養物の滴下は、ノズル
等を使用して連続的に行うことが好ましく、直径が５mm
程度で重量が50mg程度の液滴とすることが特に好まし
い。なお、滴下に際して微生物菌体培養物は、５℃以下
の温度で予め冷却しておくと良い。The microbial cell culture is preferably dropped continuously using a nozzle or the like, and the diameter is 5 mm.
It is particularly preferable that the droplets have a weight of about 50 mg. In addition, at the time of dropping, the microbial cell culture is preferably cooled in advance at a temperature of 5 ° C. or lower.
【００１３】そして、微生物菌体培養物を滴下した金属
板ごと真空乾燥した後、乾燥微生物菌体を回収しても良
いし、微生物菌体培養物の凍結粒をスクレッパー等で掻
き取ってステンレス製の網カゴ等に回収した後、真空乾
燥して乾燥微生物菌体を回収しても良い。このようにし
て、直径４mm及び高さ３mm程度の半球状で重量が５mg程
度の乾燥微生物菌体をえることができる。次に、実施例
を挙げて本発明を具体的に説明する。The microbial cell culture may be vacuum-dried together with the dropped metal plate, and then the dried microbial cells may be recovered. Alternatively, frozen particles of the microbial cell culture may be scraped off with a scraper or the like and made of stainless steel. It is also possible to collect the dried microbial cells by vacuum drying after collecting them in a net basket or the like. In this way, a dry microbial cell having a diameter of 4 mm and a height of about 3 mm and a weight of about 5 mg can be obtained. Next, the present invention will be specifically described with reference to examples.
【００１４】[0014]
【参考例１】脱脂粉乳 11.53％、酵母エキス 0.5％及び
アスコルビン酸ナトリウム 0.1％を含有する還元脱脂乳
培地 10gに、前培養したビフィドバクテリウム・ロンガ
ム(Bifidobacterium longum) SBT2928 (FERM P-10657)
3％を接種し、37℃で16時間培養して菌体培養物を得
た。[Reference Example 1] Bifidobacterium longum SBT2928 ( Bifidobacterium longum ) SBT2928 (pre-cultured) in 10 g of reduced skim milk medium containing 11.53% skim milk powder, 0.5% yeast extract and 0.1% sodium ascorbate. (FERM P-10657)
3% was inoculated and cultured at 37 ° C. for 16 hours to obtain a cell culture.
【００１５】[0015]
【実施例１】参考例１で得られた菌体培養物を５℃で１
時間以上冷却した後、−80℃の冷凍庫内で冷却しておい
た直径15cmのステンレスシャーレ上に菌体培養物 35gを
ピペット (プラスチックピペット No.7521、FALCON社
製) で滴下し、凍結した凍結粒を直径９cmの金属製網カ
ゴに回収した。なお、金属製網カゴに回収した凍結粒の
厚さは10mmであった。そして、この凍結粒を室温 (20〜
25℃) で真空乾燥し、生菌数が 7.4×108cfu/g (乾燥前
培養物換算) の乾燥微生物菌体 4.21gを得た。Example 1 The cell culture obtained in Reference Example 1 was incubated at 5 ° C. for 1 hour.
After cooling for over a period of time, 35 g of the bacterial cell culture was dropped with a pipette (Plastic pipette No.7521, manufactured by FALCON) on a stainless petri dish with a diameter of 15 cm that had been cooled in a freezer at -80 ° C. The particles were collected in a metal net basket having a diameter of 9 cm. The frozen particles collected in the metal net basket had a thickness of 10 mm. Then, freeze these frozen particles at room temperature (20 ~
After vacuum drying at 25 ° C.), 4.21 g of dried microbial cells with a viable cell count of 7.4 × 10 8 cfu / g (converted to pre-dried culture) was obtained.
【００１６】[0016]
【比較例１】参考例１で得られた菌体培養物を５℃で１
時間以上冷却した後、直径９mmのガラスシャーレに菌体
培養物 35gを入れた。なお、ガラスシャーレに入れた菌
体培養物の厚さは５mmであった。そして、このガラスシ
ャーレを冷凍庫内に５時間以上保持して菌体培養物を凍
結させた後、室温 (20〜25℃) で真空乾燥し、乾燥微生
物菌体 4.06gを得た。Comparative Example 1 The cell culture obtained in Reference Example 1 was incubated at 5 ° C. for 1 hour.
After cooling for more than an hour, 35 g of the cell culture was put in a glass petri dish having a diameter of 9 mm. The thickness of the bacterial cell culture placed in the glass petri dish was 5 mm. Then, the glass petri dish was kept in a freezer for 5 hours or more to freeze the cell culture, and then vacuum dried at room temperature (20 to 25 ° C) to obtain 4.06 g of dried microbial cells.
【００１７】[0017]
【試験例１】実施例１の乾燥微生物菌体及び比較例１の
乾燥微生物菌体を調製するに際し、経時的に (乾燥重量
／湿重量) 値を測定し、真空乾燥に要する時間を比較し
た。なお、これまでの予備実験の結果から、 (乾燥重量
／湿重量) 値が 0.120以下となったときを乾燥終了とし
た。その結果を図１に示す。[Test Example 1] When preparing the dried microbial cells of Example 1 and the dried microbial cells of Comparative Example 1, the (dry weight / wet weight) value was measured over time, and the time required for vacuum drying was compared. . From the results of the preliminary experiments so far, when the (dry weight / wet weight) value was 0.120 or less, the drying was completed. The result is shown in FIG.
【００１８】比較例１においては乾燥終了までに20時間
を要したが、実施例１においては16時間で乾燥終了とな
り、乾燥に要する時間を20％短縮することができた。ま
た、比較例１においては菌体培養物の凍結に５時間以上
の時間を要したが、実施例１においては菌体培養物を数
秒以内に凍結することができた。In Comparative Example 1, it took 20 hours to complete the drying, but in Example 1, the drying was completed in 16 hours, and the time required for drying could be shortened by 20%. Further, in Comparative Example 1, it took 5 hours or more to freeze the cell culture, but in Example 1, the cell culture could be frozen within a few seconds.
【００１９】[0019]
【試験例２】実施例１で得られた乾燥微生物菌体及び比
較例１で得られた乾燥微生物菌体をそれぞれ水に溶解
し、溶解性を目視で確認したところ、比較例１で得られ
た乾燥微生物菌体では不溶性凝縮物の残渣が多少見受け
られたが、実施例１で得られた乾燥微生物菌体では残渣
は全く見受けられず、完全に溶解していた。[Test Example 2] The dried microbial cells obtained in Example 1 and the dried microbial cells obtained in Comparative Example 1 were each dissolved in water and the solubility was visually confirmed. In the dried microbial cells, some residues of the insoluble condensate were found, but in the dried microbial cells obtained in Example 1, no residue was found and they were completely dissolved.
【００２０】[0020]
【試験例３】実施例１で得られた乾燥微生物菌体をスタ
ーターとして使用し、バルクスターターを調製した (発
明スターター) 。また、比較例として、通常の方法で継
代培養されたビフィドバクテリウム・ロンガム(Bifidob
acterium longum) SBT2928(FERM P-10657)の培養物を
スターターとして使用し、バルクスターターを調製した
(対照スターター) 。Test Example 3 Using the dried microbial cells obtained in Example 1 as a starter, a bulk starter was prepared (invention starter). In addition, as a comparative example, Bifidobacterium longum (Bifidob) subcultured by an ordinary method was used.
A bulk starter was prepared using a culture of acterium longum ) SBT2928 (FERM P-10657) as a starter.
(Control starter).
【００２１】脱脂粉乳 11.53％、酵母エキス 0.625％及
びアスコルビン酸ナトリウム0.03％を含有する還元脱脂
乳培地からなるバルクスターター調製用脱脂乳培地100g
に、実施例１で得られた乾燥微生物菌体の場合は 0.36
g、継代培養物の場合は3gを接種し、37℃で培養して、
バルクスターターの酸度と生菌数を経時的に測定した。
酸度を経時的に測定した結果を表１に、生菌数を経時的
に測定した結果を表２にそれぞれ示す。100 g of skim milk medium for bulk starter preparation consisting of reduced skim milk medium containing skim milk powder 11.53%, yeast extract 0.625% and sodium ascorbate 0.03%
In the case of the dried microbial cells obtained in Example 1, 0.36
g, in the case of subculture, inoculate 3 g, incubate at 37 ° C,
The acidity and the viable cell count of the bulk starter were measured over time.
Table 1 shows the results of measuring the acidity over time, and Table 2 shows the results of measuring the viable cell count over time.
【００２２】[0022]
【表１】 ────────────────────────────── 培養時間 ──────────────────── 14 16 18 20 (時間) ────────────────────────────── 発明スターター 0.93 1.26 1.43 1.70 対照スターター 1.76 1.98 2.13 2.32 ──────────────────────────────[Table 1] ────────────────────────────── Culture time ────────────── ────── 14 16 18 20 (hours) ────────────────────────────── Invention Starter 0.93 1.26 1.43 1.70 Control Starter 1.76 1.98 2.13 2.32 ───────────────────────────────
【００２３】[0023]
【表２】 ──────────────────────────────────── 培養時間 ────────────────────────── 14 16 18 20 (時間) ──────────────────────────────────── 発明スターター 2.0×109 2.3×109 2.3×109 2.2×109 (cfu/g) 対照スターター 2.6×109 3.4×109 3.1×109 3.2×109 ────────────────────────────────────[Table 2] ──────────────────────────────────── Culture time ──────── ────────────────── 14 16 18 20 (hours) ────────────────────────── ─────────── Invention Starter 2.0 × 10 9 2.3 × 10 9 2.3 × 10 9 2.2 × 10 9 (cfu / g) Control Starter 2.6 × 10 9 3.4 × 10 9 3.1 × 10 9 3.2 × 10 9 ────────────────────────────────────
【００２４】発明スターターは、酸度、生菌数共に対照
スターターより数値的には多少低い値を示したが、大差
は認められず、実施例１で得られた乾燥微生物菌体をス
ターターとして使用しても満足できるバルクスターター
を調製することができた。The inventive starter numerically showed a slightly lower value in both acidity and viable cell count than the control starter, but no significant difference was observed, and the dried microbial cells obtained in Example 1 were used as the starter. It was possible to prepare a satisfactory bulk starter.
【００２５】[0025]
【発明の効果】本発明の方法に従って乾燥乳酸菌スター
ターを製造することにより、真空乾燥前の凍結保持時間
を短くすることができると共に、真空乾燥の所要時間も
短くすることができる。また、真空乾燥した乾燥乳酸菌
スターターを粉砕する必要もない。さらに、中和剤や分
散媒等を添加しなくても良好な溶解性を有する乾燥乳酸
菌スターターを得ることができる。By producing a dry lactic acid bacterium starter according to the method of the present invention, the freeze holding time before vacuum drying can be shortened and the time required for vacuum drying can be shortened. Further, it is not necessary to pulverize the dried lactic acid bacterium starter dried in vacuum. Furthermore, a dry lactic acid bacterium starter having good solubility can be obtained without adding a neutralizing agent or a dispersion medium.
【図１】実施例１の乾燥微生物菌体及び比較例１の乾燥
微生物菌体を調製するに際し、経時的に測定した (乾燥
重量／湿重量) 値の変化を示す。FIG. 1 shows changes in (dry weight / wet weight) values measured with time when the dried microbial cells of Example 1 and the dried microbial cells of Comparative Example 1 were prepared.
○：実施例１の粒状乾燥微生物菌体を調製するに際し、
経時的に測定した (乾燥重量／湿重量) 値 △：比較例１の乾燥微生物菌体を調製するに際し、経時
的に測定した (乾燥重量／湿重量) 値◯: In preparing the granular dry microbial cells of Example 1,
(Dry weight / wet weight) value measured with time Δ: (dry weight / wet weight) value measured with time when preparing the dry microbial cells of Comparative Example 1
Claims (1)
て乾燥微生物菌体を製造するに際し、微生物菌体培養物
の凍結を凍結温度以下に冷却した金属板上に微生物菌体
培養物の小滴を滴下して急速凍結させることにより行う
ことを特徴とする乾燥微生物菌体の製造法。1. When producing a dried microbial cell by vacuum-drying a frozen microbial cell culture, the microbial cell culture is frozen on a metal plate which has been frozen below a freezing temperature. A method for producing dried microbial cells, which is characterized in that it is carried out by dripping small drops and rapidly freezing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8061273A JPH09248177A (en) | 1996-03-18 | 1996-03-18 | Production of dried bacterium cell body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8061273A JPH09248177A (en) | 1996-03-18 | 1996-03-18 | Production of dried bacterium cell body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH09248177A true JPH09248177A (en) | 1997-09-22 |
Family
ID=13166450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8061273A Pending JPH09248177A (en) | 1996-03-18 | 1996-03-18 | Production of dried bacterium cell body |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH09248177A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001021187A1 (en) * | 1999-09-17 | 2001-03-29 | Takeda Chemical Industries, Ltd. | Process for producing protein powder |
| WO2010106095A1 (en) | 2009-03-19 | 2010-09-23 | Intervet International B.V. | In situ constituting a vaccine for administration to a predetermined herd of animals |
| US8516714B2 (en) | 2008-01-21 | 2013-08-27 | Intervet International B.V. | Method for lyophilising particles having a pharmaceutical compound contained therein and a pharmaceutical pack containing such particles |
-
1996
- 1996-03-18 JP JP8061273A patent/JPH09248177A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001021187A1 (en) * | 1999-09-17 | 2001-03-29 | Takeda Chemical Industries, Ltd. | Process for producing protein powder |
| US6723347B1 (en) | 1999-09-17 | 2004-04-20 | Takeda Chemical Industries, Ltd. | Proces for producing protein powder |
| US8516714B2 (en) | 2008-01-21 | 2013-08-27 | Intervet International B.V. | Method for lyophilising particles having a pharmaceutical compound contained therein and a pharmaceutical pack containing such particles |
| WO2010106095A1 (en) | 2009-03-19 | 2010-09-23 | Intervet International B.V. | In situ constituting a vaccine for administration to a predetermined herd of animals |
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