JPH09238674A - Striking device for introducing biological substance or physiologically active substance - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a striking device capable of supplying sufficient acceleration in introducing a biological substance or a physiologically active substance into an organism in a small-scale apparatus under low pressure without contaminating and damaging the organism with a pressurized fluid. SOLUTION: This device comprises an airtight cylinder 3 whose on end is connected to a pressurized fluid source 6, an emitting material 10 set in the cylinder 3, a stopper 25 which is set at another end of the cylinder 3 and stops the movement of the emitting material 10, a flow channel 25 laid between the pressurized fluid source 6 and the cylinder 3 and an on-off valve 22 attached to the flow channel 25. A biological substance or a physiologically active substance is supported on a face opposite to a face with which the pressurized fluid is brought into contact, the on-off valve 22 is opened and the emitting material 10 is moved to stop the stopper 15. The biological substance or the physiologically active substance is directly emitted from the emitting material 10 by the shock of the stopping.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a driving apparatus for introducing a biological substance such as DNA or chromosome or a physiologically active substance into a living body such as a biological tissue or a biological cell.

[0002]

2. Description of the Related Art As a conventional implanting device for introducing a biological substance or a physiologically active substance into an organism such as a biological tissue or a biological cell, there is one disclosed in Japanese Patent Laid-Open No. 5-508316. It is as shown in FIGS. 14-18, and includes a high pressure gas delivery system 30, a piston 32 with a rod 32a, a high pressure chamber 33, and a first high pressure chamber 33 for generating an instantaneous shock wave from the high pressure gas.
A shock mechanism 31 having a membrane 34 and a housing 35 for releasing, accommodating and ventilating the shock wave, and a compatible insertion can be performed, and the gas shock wave generated by the shock mechanism 31 is converted into acceleration of a small projectile. A throat region 37 having a second membrane 36 which can be connected to a compatible nozzle 38 for targeting various types of biological targets (organisms) 8.
And include.

An inert gas such as helium gas is used as the high pressure gas, and the gas is pressurized (200 kg /
cm ^2,) and is housed in a gas container of a high pressure gas delivery system 30 has a pressure fluid source 6.

Further, the piston 32 is made of a magnetically responsive metal, and the piston 32 and the rod 32a are provided with a pressurized gas (pressure fluid 6a) supplied from the pressure fluid source 6 to the high pressure chamber 33 below. It is hollow or has a groove so that it can pass through. Reference numeral 41 is a vacuum means for evacuating the housing 35, 50 is an electric urging mechanism for urging the piston 32 so as to pierce the first film 34 with the rod 32a, and 51 is urged by the electric urging mechanism 50. Rod 32a with piston 32
Is a solenoid for moving.

In such a case, the biological substance 7
Alternatively, when the physiologically active substance 7 ′ is introduced into the living body 8 such as a biological tissue or a biological cell, the high pressure gas is introduced into the high pressure chamber 33, and the piston 32 is urged by the electric urging mechanism 50 to move. The rod provided on the piston 32
The high pressure chamber 33 is formed by making a hole in the first membrane 34 by the sharp tip of 32a.
The high pressure gas is discharged from inside as the pressure fluid 6a,
As shown in FIGS. 15 to 17, the gas shock wave generated by this discharge is momentarily introduced into the throat region 37 and opened outward, and is attached in the throat region 37 so as to face the organism 8. , A biological substance 7 such as DNA or a physiologically active substance 7 ', and a mixture of fine particles of about 1 μm (usually called a carrier) such as gold or tungsten, which is carried on the surface facing the organism 8, or a polyimide or The surface of the second film 36 made of polyester, which is opposite to the surface facing the organism 8, is impacted, and the second film 36 is ruptured by the impact at that time and the mixture attached to the second film 36 It is fired and introduced into the organism 8.

In the above-mentioned structure, when the second membrane 36 is hit, the second membrane 36 is struck by the high pressure of the pressure fluid 6a.
The crushed debris and the ejected pressure fluid 6a are
The biological substance 7 or the physiologically active substance 7'is released to the side.
There is a problem that it collides with the living body 8 into which it is introduced and contaminates or damages the living body 8. As a solution to this problem, the second film 36 is inserted above the throat region 37 as shown in FIG.
A screen 39 is provided between the second body 36 and the living body 8 so that the second film 36 is expanded but not ruptured, and only the biological substance 7 or the physiologically active substance 7'is allowed to pass therethrough for emission. is there.

[0007]

In the driving device provided with the conventional screen as described above, the passage rate of the biological substance or the physiologically active substance is lowered and the pressure introduced into the organism is lost. Therefore, there is a problem that the device must be large.

Therefore, an object of the present invention is to solve the problems of the conventional driving device as described above, to make the device compact, and to reduce the pressure of the pressure fluid for ejecting a biological substance or a physiologically active substance. Even if it is low, the introduction pressure can be sufficiently obtained, and the organism into which the biological substance or the physiologically active substance is introduced is not contaminated or damaged by the pressure fluid or the debris of the membrane. An object of the present invention is to provide a driving device that does not cause a loss in the introduction pressure of the.

[0009]

In order to achieve the above-mentioned object, the present invention according to claim 1 provides an airtight cylinder having a pressure fluid source connected to one end thereof, A projectile provided in the tubular body, a stopper provided at the other end of the tubular body to stop the movement of the projectile, a flow passage provided between the pressure fluid source and the tubular body, and a flow passage provided in the flow passage. And an on-off valve, a biological substance or a physiologically active substance is carried on the opposite surface of the projectile against which the pressure fluid contacts, the on-off valve is opened, the projectile is moved, and stopped by a stopper. It is characterized in that a biological substance or a physiologically active substance is directly emitted from this projectile by the impact of. Claim 2
The invention described in (1), the airtight cylinder body having a pressure fluid source connected to one end, a projectile provided in the cylinder body, a housing for installing the cylinder body, and the other end of the cylinder body. And has a stopper for stopping the movement of the projectile, a flow passage provided between the pressure fluid source and the cylinder, and an on-off valve provided in this flow passage,
A biological substance or a physiologically active substance is carried on the opposite surface of the projectile against which the pressure fluid abuts, the on-off valve is opened, the projectile is moved and stopped by a stopper, and the impact of this stop causes the biological substance or The feature is that the physiologically active substance is directly emitted from this projectile. According to a third aspect of the present invention, in the second aspect of the present invention, the housing has a vacuum means for evacuating the inside of the housing as necessary. The invention according to claim 4 is the invention according to any one of claims 1 to 3, wherein the pressure fluid is a pressurized inert gas, air, water, oils, or a blast caused by an explosion of explosives. It is characterized by. According to a fifth aspect of the invention, in the invention according to any one of the first to fourth aspects, an opening is provided in each of the side walls on one end side and the other end side of the tubular body, and the projectile moves to cause a stopper inside the tubular body. When stopped at, the conduits for leading out the fluid in the cylinder discharged from the respective openings are connected to the respective openings. According to a sixth aspect of the invention, in the fifth aspect of the invention, the conduit has a vacuum means for evacuating the inside of the cylinder, if necessary. The invention according to claim 7 is the invention according to any one of claims 1 to 5, characterized in that the impact pressure of the projectile is 1 to 50 kg / cm ² .
The invention according to claim 8 is characterized in that, in the invention according to any one of claims 1 to 5, the projectile is made of a material having a strong elastic force such as a titanium alloy.
The invention described in claim 9 is characterized in that, in the invention described in any one of claims 1 to 5, the projectile is made of a material having a large specific gravity such as lead, iron, and depleted uranium. The invention described in claim 10 is the invention according to any one of claims 1 to 5, characterized in that the projectile has an O-ring. According to the invention of claim 11,
In the invention according to any one of claims 1 to 5, the projectile comprises a titanium alloy layer and a magnetic material layer. The invention described in claim 12 is,
In any one of the inventions described above, the projectile is composed of a titanium alloy layer and a metal layer of lead, iron, depleted uranium or the like. The invention according to claim 13 is characterized in that, in the invention according to any one of claims 1 to 5, the projectile comprises a metal layer of lead, iron, depleted uranium or the like and a plastic material layer. is there. The invention described in claim 14 is the invention described in any one of claims 1 to 5, characterized in that the projectile is made of a plastic material. According to a fifteenth aspect of the present invention, in the invention according to any one of the first to fifth aspects, the surface of the projectile carrying the biological substance or the physiologically active substance is subjected to honing or ion sputtering. It is characterized by.

[0010]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A first embodiment of the present invention is shown in FIG.
4, the airtight cylinder 3 is connected to the pressure fluid source 6, the first cap 4 is attached to one end of the cylinder 3, and the projectile is provided in the cylinder 3. 10 and the projectile
When the 10 is placed in the predetermined position of the first cap 4, the projectile
Above the surface (rear surface) 11 that contacts the pressure fluid of 10,
An exhaust pipe 13b provided on the side wall of the cylinder 3 and having an exhaust valve 22 'for discharging the supplied pressure fluid 6a and a second cap 5 at a predetermined position when the projectile 10 moves in the cylinder 3. A stopper 15 for stopping the projectile 10 and an opening 13 provided at the other end of the tubular body 3 and communicating with an exhaust hole 13a for discharging the fluid in the tubular body 3 when the projectile 10 contacts the stopper 15. A second cap 5 attached to the other end of the tubular body 3, a channel 25 provided between the first cap 4 and the pressure fluid source 6, and an on-off valve 22 provided in the channel 25. A timer 23 for adjusting the opening time of the on-off valve 22 is also installed.

After the exhaust valve 22 'projectile 10 comes into contact with the stopper 15, the pressure fluid 6a existing in the cylindrical body 3 is opened.
To discharge. The exhaust valve 22 'is a manual valve member, or
Not limited to this, the detection means (not shown) detects the contact of the projectile 10 with the stopper 15, and the electromagnetic valve is opened by the detection output.

The projectile 10 is made of a material having a large specific gravity, such as lead, iron, and depleted uranium.
It can be installed at any position in the cylinder 3 so that the pressure fluid 6a does not leak from between 10 and the inner wall of the cylinder 3, and the stroke can be adjusted. A seal ring 12 is attached to improve its durability. Then, the cylindrical body 3 and the first and second caps 4 and 5 are formed so that the stroke of the projectile 10 is about 20 cm.

The surface of the projectile 10 on which the biological substance 7 (DNA or the like) or the physiologically active substance 7'is carried is formed into a flat plate shape and subjected to a honing process to form a ground glass. Not limited to honing, ion sputtering may be used.

As the pressure fluid 6a, any one of pressurized inert gas, air, water, oils and the like is used, and when the diameter of the projectile 10 is 2 cm ² and the fluid pressure is 10 kg / cm ² , The impact energy when projectile 10 collides with stopper 15 is
It is about 10kg / Fm, but the diameter of the projectile 10 and the pressure fluid 6
The impact pressure of the projectile 10 is adjusted within the range of 1 to 50 kg / cm ² by appropriately selecting the pressure a, the supply amount of the pressure fluid 6a, and the stroke.

As the pressure fluid 6a, instead of pressurized inert gas or the like, explosive powder is contained in the first cap so that the impact pressure of the projectile 10 on the rear surface 11 of the projectile 10 becomes desired. Alternatively, the projectile 10 may be fired by loading an appropriate amount of it on the vehicle and exploding it by using an ignition means such as electric ignition.

The first cap 4 and the second cap 5 are screwed and attached to both ends of the cylindrical body 3. Further, the inner diameters of the first cap 4 and the second cap 5 and the inner diameter of the tubular body 3 are formed to be the same so that the projectile 10 can be moved smoothly. Then, the first cap 4 and the second cap 5 are removed, the projectile 10 is inserted into the first cap 4 or a predetermined position in the tubular body 3, and then the first cap 4 and the second cap 5 are removed. It is attached to the cylinder 3 to prepare for the firing of the projectile 10. At this time, the projectile 10 is held at a predetermined position by the spring action of the seal ring 12 attached to the projectile 10.

The means for supplying the pressure fluid 6a is provided with an opening / closing valve 22 composed of an electromagnetic valve in a flow passage 25 provided between the first cap 4 and the pressure fluid source 6 pressurized to a predetermined pressure. It is a thing. Then, the opening / closing valve 22 is opened by a timer 23 for a predetermined time so that a desired impact force is obtained, and a set amount of the pressure fluid 6a is supplied.

When such a thing is used, as shown in FIG. 2, it is set on the fixed table 29 and supplied with the pressure fluid 6a to operate it, or the target organism 8 is used.
Depending on the case, as shown in FIG. 3, it is set on the holding member 29 'to hold the tubular body 3 by hand, or simply holding it by hand and supplying the pressure fluid 6a to operate it.

In such a structure, the surface of the projectile 10 carrying the biological substance 7 or the physiologically active substance 7 ', the biological substance 7 or the physiologically active substance 7', and gold of about 1 μm or A fine particle substance (carrier) such as tungsten is mixed and carried in a liquid medium such as water, and the opening / closing valve 22 is operated for a predetermined time while the exhaust valve 22 'is closed, so that the projectile 10
Is installed at a predetermined position in the cylindrical body 3, the pressure fluid 6a is supplied from the back surface 11 of the projectile 10, the projectile 10 is moved and suddenly stopped by the stopper 15, and the collision causes the projectile 10 The biological substance 7 or the physiologically active substance 7 ′, the fine-grained substance and the liquid medium are directly emitted from the projectile 10 and introduced into the organism 8 by applying an impacting force to. In this case, since the surface of the projectile 10 carrying the biological substance 7 or the physiologically active substance 7'is a flat plate shape, the radiation angle of flight is concentrated without spreading as shown in FIG. It can be introduced deep into the central part of the body 8 and shallow into the peripheral part.

Further, the air remaining in the cylindrical body 3 or the preceding pressure fluid is passed through the exhaust hole 13a to be opened through the opening 13, and the newly supplied pressure fluid 6a is opened through the exhaust valve 22 '. Then, they are discharged from the exhaust pipe 13b respectively to prepare for the subsequent impact operation. Because of this, there is no gap between the projectile 10 and the cylinder 3 due to the seal ring 12, and the pressure fluid 6
The a does not leak and hit the organism 8 directly, and the organism 8 is not damaged or contaminated by the pressure fluid 6a.

Since the surface of the projectile 10 carrying the biological substance 7 or the physiologically active substance 7'has been subjected to honing processing, the biological substance 7 or the physiologically active substance 7'and about 1 μm of gold, platinum or tungsten, etc. It is possible to surely carry the granular material and the liquid material such as water at a predetermined position without diffusing on the surface of the projectile 10.

Since the projectile 10 is made of a material having a large specific gravity such as lead, iron or depleted uranium, the kinetic energy of the projectile 10 can be increased, and the projectile 10 can be a stopper.
The hitting force when hitting 15 is great.

Although not shown in the drawings, the second embodiment does not include the first embodiment.
In a structure similar to that of the embodiment, the projectile 10 is made of a titanium alloy having extremely strong elasticity. Such projectile 10 does not lose impact energy due to its elasticity, and is further subjected to a composite impact pressure by the action of elastic force, the impact force is doubled and converted into vibration, and fine particles , Is transmitted to the biological substance 7 or the physiologically active substance 7 ', accelerates them to fly and collide with the target organism 8, and the accelerating force penetrates the cell wall membrane of the organism 8 and introduces them. Can be a projectile
When the 10 is fired, the projectile 10 stops at the predetermined position.
When it is received by 15, it does not break, the pressure fluid 6a does not leak, does not contaminate the organism 8 or damages it, and the reliability of the device is extremely high.

Although not shown, the third embodiment is the first
In a structure similar to that of the embodiment, the projectile 10 is composed of a titanium alloy layer and a magnetic material layer. In such a case, the biological substance 7 or the physiologically active substance 7 ′, the liquid medium, and the magnetic fine particles carrying the gene as disclosed in JP-A-6-133784 (known in the art) ( The mixture with the carrier) can be held securely on the projectile 10 by magnetism. The order of stacking the titanium alloy layer and the magnetic material layer may be a titanium alloy layer, a magnetic material layer, or vice versa in the direction of the biological substance 7 or the physiologically active substance 7 ', and any one of them may be appropriately selected.

Although not shown in the drawings, the fourth embodiment is the first
In the same structure as the embodiment, the projectile 10 is composed of a titanium alloy layer and a metal layer made of a material having a large specific gravity such as lead, iron, and depleted uranium. With such a structure, the kinetic energy of the projectile 10 can be increased, the impact energy is not lost due to the elasticity of the titanium alloy layer, and the composite impact pressure is applied by the action of the elastic force. , The impact force is doubled and converted into vibration, and the acceleration that causes the biological substance 7 or the physiologically active substance 7'and the liquid medium or the like to fly is larger than that of the projectile 10 composed of a single substance. Become. The order of stacking the titanium alloy layer and the metal layer is such that the one carrying the biological substance 7 or the physiologically active substance 7 ′ has the titanium alloy layer,
It may be a metal layer or vice versa, and either one is appropriately selected.

Although illustration is omitted, the fifth embodiment is the first embodiment.
In the same structure as the embodiment, as shown in FIG. 5, the projectile 10 includes a metallic material layer 10a such as lead, iron, depleted uranium, and titanium alloy, and an elastic plastic layer 10b such as polyimide or polyester. It consists of That is, the plastic layer 10b is arranged on the side carrying the biological substance 7 or the physiologically active substance 7 ', and a concave portion is provided in the metal material layer 10a of the projectile 10, and the convex portion which is tightly fitted in the concave portion is formed by the plastic layer. It is provided on 10b, and both are engaged and integrally assembled. In such a case, the plastic layer 10b can be charged and the biological substance 7 or the physiologically active substance 7'and the liquid medium can be adsorbed and reliably held, and when the projectile 10 is launched. The static electricity is removed to make it easier to fly. It should be noted that the metal material layer 10a and the plastic layer 10b are not engaged by the above-described strong fitting of the concave portion and the convex portion, but as shown in FIG. The joint surface may be formed into a flat plate shape, and the both surfaces may be bonded with an adhesive material.

Although illustration is omitted in the sixth embodiment, the first embodiment
In the structure having the same structure as that of the embodiment, the projectile 10 is replaced with a titanium alloy and an elastic resin such as polyimide or polyester is used. In such a thing, the projectile 10 can be formed to be lightweight,
In addition, it is easy to process, and the biological material 7 or the physiologically active material 7'is charged by charging the plastic material layer,
The liquid medium can be adsorbed and held securely, and when the projectile 10 is launched, static electricity is removed to facilitate flight.

The seventh embodiment is a projectile 10 of the first embodiment.
Instead of the flat plate-shaped surface for supporting the biological substance 7 or the physiologically active substance 7'of FIG. 7, the convex-shaped curved face is formed as shown in FIG. In such a case, when the biological substance 7 or the physiologically active substance 7'is made to fly, the surface of the projectile 10 is formed to have a convex curved surface as compared with that of the first embodiment. As shown in, the flight of the biological substance 7 or the physiologically active substance 7'is radiated with an appropriate spread and can be introduced into a wide range of the organism 8. The shape of the surface of the projectile 10 for supporting the biological substance 7 or the physiologically active substance 7'is not limited to the flat plate shape or the convex curved surface shape, and may be a convex shape or a concave shape so as to meet the introduction condition into the organism 8. It may be formed into a curved surface or a concave surface.

The eighth embodiment differs from the first embodiment in that
As shown in FIG. 8, an opening 13 and an exhaust pipe 13b are provided at one end and a side wall of the other end of the cylindrical body 3, respectively, and the conduit 13 is provided by connecting the opening 13 and the exhaust pipe 13b. With such a structure, when the projectile 10 moves and stops at the stopper 15 in the cylindrical body 3, the residual air discharged from the cylindrical body 3 and the supplied pressure fluid 6a are delivered to a desired location. It can be released to protect organisms and workers from the exhaust fluid. If necessary, a vacuum means 18 for evacuating the inside of the cylindrical body 3 may be provided in the conduit 14. When the inside of the cylindrical body 3 is evacuated, the projectile 10 is smoothly moved to the diaphragm 9, and the impact sound when the projectile 10 collides with the diaphragm 9 can be reduced.

The ninth embodiment differs from the first embodiment in that
As shown in FIG. 9, a casing 2 in which a tubular body 3 having an outlet 26 having an opening / closing valve and an air supply port 26 'having an opening / closing valve is installed.
Is provided. As the on-off valve provided at the air supply port 26 ', a manual valve or a solenoid valve which opens and closes by a command signal is used. Further, a soundproofing material may be provided on the outer peripheral surface or the inner wall surface of the housing 2, and a light source 50 necessary for growing the living body 8 may be provided in the housing 2.
Further, instead of manually setting the projectile 10 at a predetermined position of the first cap 4, as shown in FIG. 10, a so-called linear motor type moving device using electromagnetic force, or an electromagnetic force (not shown) is used. The elevator mechanism using the above may be installed in the cylindrical body 3 and moved.

In such a structure, the existing fluid and the pressure fluid 6a in the cylindrical body 3 discharged from the opening 13 and the exhaust pipe 13b are directed to the lateral wall of the housing 2 and flow into the housing 2. It is discharged, and further discharged from the housing 2 through the discharge port 26 of the housing 2. At this time, the fluid is not suitable for the organism 8,
The organism 8 is not contaminated or damaged. further,
In the preparation process for using this device, the gas (CO ₂ ,
_{O 2, NO, NO 2,} SO 2, after supplying the required amount, etc.) steam, and closes the on-off valve, also create an environment condition from the light source 50 is irradiated with light, the organism 8, and biological Make sure that the substance 7 or the physiologically active substance 7'is not dried or killed. In this case, the opening / closing valve of the discharge port 26 is closed, the pressure fluid 6a is supplied, and after the projectile 10 collides with the stopper 15, the opening / closing valve of the discharge port 26 is opened to discharge the pressure fluid 6a.

Since the housing 2 is filled with a gas such as CO ₂ , the discharged pressure fluid 6a does not damage the living organism 8. And, the one provided with the soundproof material around the cylindrical body 3 or around the second cap is the cylindrical body 3
Prevents noise when the existing fluid inside is drained.

The tenth embodiment is the same as the eighth embodiment.
As shown in FIG. 11, a vacuum means 17 is provided at the discharge port 26 of the housing 2. In such a case, if the inside of the housing 2 is evacuated as needed, the impact force of the projectile 10 can be enhanced and the collision force can be increased, as compared with the case where it is not a vacuum.
Moreover, the noise of collision between the projectile 10 and the stopper 15 and the noise of discharge of the pressure fluid are reduced, and no noise pollution occurs. or,
After the inside of the housing 2 is evacuated and the biological substance 7 or the physiologically active substance 7'is introduced into the organism 8, a leak valve (not shown) for returning the inside of the housing 2 to normal pressure is provided. 2, it is preferable to further provide a filter for removing contaminants in the outside air on the front side or the rear side of the leak valve of the flow path through which the outside air flows.

The eleventh embodiment is different from the ninth embodiment in that
As shown in FIG. 12, a conduit 14 for discharging the air, the existing fluid, or the pressure fluid 6a in the cylindrical body 3 discharged from the opening 13 and the exhaust pipe 13b to the outside of the housing 2 is passed through the housing 2. It was provided by. In such a case, the air inside the cylinder 3, the existing fluid, or the pressure fluid 6a supplied at the time of operation can be directly discharged to the outside of the housing 2 by the conduit 14, and the inside of the housing 2 is If the inside of the casing 3 is not contaminated and the inside of the housing 2 is not evacuated, but the inside of the cylinder 3 is evacuated, the impact of the projectile 10 can be enhanced and the collision force can be increased. It is possible to introduce the biological substance 7 or the physiologically active substance 7'into the organism 8 in a normal state without being suffocated while breathing, such as an animal, since the water content of the organism 8 does not evaporate and deteriorate. The outlet of the conduit 14 may be provided with a filter for purifying the discharged fluid.

The twelfth embodiment is the same as the ninth embodiment.
As shown in FIG. 13, a vacuum means 18 is provided outside the housing 2 and in the middle of the conduit 14. This vacuum means 18 increases the collision force between the projectile 10 and the stopper 15, and reduces the discharged pressure fluid 6a and the noise such as the impact sound between the projectile 10 and the diaphragm 9. Note that a muffling means 16 ′ may be provided outside the housing 2 and in the middle of the conduit 14 and between the housing 2 and the vacuum means 18. This silencer 1
6'is a muffler for muffler, which discharges the pressure fluid 6
The noise such as the impact sound of the projectile 10 and the diaphragm 9 is further reduced.

The tenth embodiment, the eleventh embodiment,
The twelfth embodiment may be appropriately combined and used.

[0037]

As described above, the present invention according to claim 1 provides an airtight cylinder having a pressure fluid source connected to one end thereof, and a projectile provided in the cylinder. A stopper provided at the other end of the tubular body for stopping the movement of the projectile, a flow passage provided between the pressure fluid source and the tubular body, and an on-off valve provided in the flow passage, A biological substance or a physiologically active substance is carried on the opposite surface of the projectile against which the pressure fluid abuts, the on-off valve is opened, the projectile is moved and stopped by a stopper, and the impact of this stop causes the biological substance or Since the physiologically active substance is directly emitted from this projectile, the device can be made small and robust, the operation is simple, the steam sterilization and dry heat sterilization of the diaphragm are easy, and the pressure of the pressure fluid is A projectile carrying a biological or bioactive substance, which can be low The biological material or physiologically active substance is not crushed and is not contaminated with the pressure fluid and various bacteria in the pressure fluid without directly contacting the pressure fluid with the organism to be driven, nor blown away the organism. Can be introduced into an organism, and the effect is that the organism is not damaged. According to a second aspect of the present invention, an airtight cylinder having a pressure fluid source connected to one end, a projecting body provided in the cylinder, a housing for installing the cylinder, and another cylinder It has a stopper provided at the end to stop the movement of the projectile, a flow passage provided between the pressure fluid source and the cylindrical body, and an opening / closing valve provided in this flow passage, and A biological substance or a physiologically active substance is carried on the opposite surface to be abutted, the opening / closing valve is opened, the projectile is moved and stopped by the stopper, and the impact of this stop causes the biological substance or the physiologically active substance to be emitted. Since it is radiated directly from the body, the device can be manufactured compactly and robustly, and it can be shielded from the outside air to prepare the environment of organisms and biological or physiologically active substances, and it is easy to operate. Yes, steam sterilization and dry heat sterilization of projectiles and cylinders are easy May lower the pressure in the pressure fluid, projectile are not crushed carrying biological substances or biologically active substances, directly into the implanted is an organism,
Without contact with the pressure fluid, it is not contaminated with the pressure fluid and various bacteria in it, and does not blow away the organism, and highly infectious microorganisms can be introduced into the organism, and It has the effect of not being damaged. According to the third aspect of the present invention, the housing has a vacuum means for evacuating the inside of the housing as necessary. Therefore, normally, the vacuum means is not operated and the normal pressure in the housing is maintained, and the housing is easily damaged. Samples such as organisms and DNA can be introduced without damaging, and can be implanted into an object that is breathing like an animal in a normal state without being suffocated. It is possible to avoid evaporation of water such as the active substance, and to increase the collision force of the projectile by making a vacuum if necessary, and reduce the impact sound between the projectile and the stopper. There is an effect. The invention described in claim 4 is
Since the pressurized fluid is a pressurized inert gas, air, water, oil, or a blast caused by the explosion of explosives, the pressurized fluid is inexpensive and can be easily obtained. According to a fifth aspect of the present invention, an opening is provided in each of the side walls on the one end side and the other end side of the tubular body, and when the projectile moves and is stopped by the stopper in the tubular body, the tubular body is discharged from each of the openings. Since the conduits for discharging the fluid in the body are connected to the respective openings, the fluid discharged from the tubular body 3 can be discharged to a desired place, and the organism and the worker are protected from the fluid. There is an effect that can be done. In the invention according to claim 6, the conduit has vacuum means for evacuating the inside of the cylinder as necessary, so that the inside of the cylinder can be evacuated when necessary, and the air resistance is lowered to reduce the projectile. The impact speed can be reduced and the impact noise can be reduced. In the invention according to claim 7, since the impact pressure of the projectile is 1 to 50 kg / cm ² , the device can be downsized, and it can be handheld such as a gun type, during surgery. It has the effect of being applicable to the human body. According to the invention described in claim 8, since the projectile is made of a material having a strong elastic force such as a titanium alloy, the projectile is not crushed by the impact when it comes into contact with the stopper, and its vibration characteristic is appropriate. There is high reproducibility of introduction, and
There is an effect that the acceleration of the biological substance or the physiologically active substance can be doubled and caused to fly. In the invention according to claim 9, since the projectile is made of a material having a large specific gravity such as lead, iron, and depleted uranium, a large kinetic energy can be obtained when the projectile is contacted with the stopper. It has the effect of having a large impact force. In the invention according to claim 10, since the projectile has an O-ring, the pressure fluid inside the cylinder does not unnecessarily leak outside the cylinder, living organisms are not contaminated, and damage to the cylinder inner wall is prevented. Therefore, the durability of the projectile is improved, the mounting position of the projectile can be set arbitrarily, and the impact force of the projectile can be adjusted appropriately. According to the invention described in claim 11, since the projectile comprises the titanium alloy layer and the magnetic material layer, it is possible to surely hold the biological substance or the physiologically active substance on the projectile together with the carrier using the magnetic substance. There is an effect. In the invention according to claim 12, the projectile is a titanium alloy layer and lead, iron,
Since it is composed of a metal layer such as depleted uranium, a large amount of kinetic energy can be obtained when it is launched, and the impact force when the projectile contacts the stopper is large. In the invention according to claim 13, since the projectile is composed of a metal layer such as lead, iron, and depleted uranium and a plastic material layer, the plastic material layer is charged to carry a biological substance or a physiologically active substance, Further, there is an effect that it is possible to easily remove the electric charge and radiate it. In the invention according to claim 14, since the projectile is made of a plastic material, the projectile can be made inexpensive, and the projectile is charged to carry a biological substance or a physiologically active substance, and the charge is removed. The effect is that it can be easily emitted. According to the invention of claim 15, in which the surface of the projectile supporting the biological substance or the physiologically active substance is subjected to honing treatment or ion sputtering treatment, the supported biological substance or the physiologically active substance is diffused. There is an effect that it can be surely held.

[Brief description of drawings]

FIG. 1 is a schematic vertical sectional view of a first embodiment of the present invention.

FIG. 2 is a diagram showing a state where the first embodiment is installed on a fixed base.

FIG. 3 is a diagram showing a state in which the first embodiment is set on a holding member.

FIG. 4 is a diagram showing a flying state of a biological substance or a physiologically active substance according to the first embodiment.

FIG. 5 is a side view of a projectile of the fifth embodiment of the above.

FIG. 6 is a side view of another projectile of the fifth embodiment of the above.

FIG. 7 is a schematic vertical sectional view of a cylindrical portion of a seventh embodiment of the above.

FIG. 8 is a schematic vertical sectional view of an eighth embodiment of the above.

FIG. 9 is a schematic vertical sectional view of a ninth embodiment of the above.

FIG. 10 is a schematic vertical cross-sectional view in which a linear motor is provided on the cylindrical portion of the eighth embodiment.

FIG. 11 is a schematic longitudinal sectional view of the tenth embodiment.

FIG. 12 is a schematic vertical sectional view of an eleventh embodiment of the above.

FIG. 13 is a schematic vertical sectional view of the twelfth embodiment.

FIG. 14 is a schematic vertical sectional view of a conventional driving device.

FIG. 15 is a schematic vertical cross-sectional view showing a state in which the above membrane carries a biological substance or a physiologically active substance.

FIG. 16 is a schematic vertical cross-sectional view showing a state in which the above-mentioned membrane is projected toward an organism by a pressure fluid.

FIG. 17 is a schematic vertical cross-sectional view showing a state in which the above-mentioned membrane is ruptured and a biological substance or a physiologically active substance is accelerated and flies toward an organism.

FIG. 18 is a schematic vertical cross-sectional view showing a state in which the above screen prevents the membrane from bursting.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Driving device 2 Housing 3 Cylindrical body 4 1st cap 5 2nd cap 6 Pressure fluid source 7 Biological substance 7'Biologically active substance 8 Biological body 10 Projector 13 Opening 14 Conduit 15 Stopper 17, 18 Vacuum means 22 Open / close valve 25 flow paths

Claims (15)

[Claims] 1. A gas-tight cylinder having a pressure fluid source connected to one end thereof, a projectile provided in the cylinder, and a stopper provided at the other end of the cylinder for stopping the movement of the projectile. , A flow path provided between the pressure fluid source and the cylindrical body, and an on-off valve provided in this flow path, and a biological substance or a physiologically active substance is provided on the opposite surface of the projectile against which the pressure fluid abuts. A biological substance characterized in that it is carried, the on-off valve is opened, the projectile is moved and stopped by a stopper, and the impact of this stop causes the biological substance or physiologically active substance to be directly emitted from this projectile. Alternatively, a driving device for introducing a physiologically active substance. 2. An airtight cylinder having a pressure fluid source connected to one end, a casing for installing the cylinder, and a stopper provided at the other end of the cylinder for stopping the movement of the projectile. An opposite surface on which the pressure fluid of the projecting body abuts, a projecting body provided in the cylindrical body, a flow passage provided between the pressure fluid source and the cylindrical body, and an opening / closing valve provided in this flow passage. The biological substance or physiologically active substance is loaded on the substrate, the on-off valve is opened, the projectile is moved and stopped by the stopper, and the biological substance or physiologically active substance is directly emitted from the projectile by the impact of this stop. An implanting device for introducing a biological substance or a physiologically active substance, which is characterized by: 3. The implanting device for introducing a biological substance or a physiologically active substance according to claim 2, wherein the casing has vacuum means for evacuating the inside of the casing if necessary. 4. The biological system according to claim 1, wherein the pressure fluid is a pressurized inert gas, air, water, oils, or a blast caused by the explosion of an explosive. A driving device for introducing a substance or a physiologically active substance. 5. An opening is provided in each of the side wall on one end side and the other end side of the cylindrical body, and when the projectile moves and is stopped by a stopper in the cylindrical body, the fluid in the cylindrical body discharged from each of the openings is discharged. The implanting device for introducing a biological substance or a physiologically active substance according to any one of claims 1 to 4, characterized in that a conduit to be led out is connected to each of the openings. 6. The implanting device for introducing a biological substance or a physiologically active substance according to claim 5, wherein the conduit has a vacuum means for evacuating the inside of the cylinder if necessary. 7. The driving device for introducing a biological substance or a physiologically active substance according to claim 1, wherein the impact pressure of the projectile is 1 to 50 kg / cm ² . 8. The implant for introducing a biological substance or a physiologically active substance according to any one of claims 1 to 5, wherein the projectile is made of a material having a strong elastic force such as a titanium alloy. apparatus. 9. The projectile is made of a material having a large specific gravity such as lead, iron, and depleted uranium.
An implanting device for introducing the biological substance or the physiologically active substance according to any one of 5 above. 10. The driving apparatus for introducing a biological substance or a physiologically active substance according to claim 1, wherein the projectile has an O-ring. 11. The driving device for introducing a biological substance or a physiologically active substance according to claim 1, wherein the projectile comprises a titanium alloy layer and a magnetic material layer. 12. The biological substance or physiologically active substance according to claim 1, wherein the projectile comprises a titanium alloy layer and a metal layer of lead, iron, depleted uranium or the like. Driving device to be introduced. 13. The biological substance or physiologically active substance according to claim 1, wherein the projectile comprises a metal layer of lead, iron, depleted uranium or the like and a plastic material layer. Driving device to be introduced. 14. The driving device for introducing a biological substance or a physiologically active substance according to any one of claims 1 to 5, wherein the projectile is made of a plastic material. 15. The biological device according to claim 1, wherein the surface of the projectile carrying the biological substance or the physiologically active substance is subjected to honing treatment or ion sputtering treatment. A driving device for introducing a substance or a physiologically active substance.