JPH09191854A - Food and drink containing cholesterol reducing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain food and drink containing a cholesterol reducing agent and capable of easily ingesting by adding a microorganism cell-cultured product to a single or various foods and drinks without troublesome processes such as separation, harvesting, washing, etc. SOLUTION: This food and drink containing the dried and concentrated material of a cultured product as a cholesterol reducing ingredient is obtained by culturing one or two microorganisms selected from the group consisting of Streptococcus faecium, Streptococcus faecalis, Streptococcus avium, Streptococcus salivarius, Streptococcus durans, Streptococcus mitis and Streptococcus equinus in an ingestible medium having one or two materials selected from the group consisting of a milk-based material and a bean-based material and concentrating, then drying the cultured product.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a food or drink containing a cholesterol lowering agent.

[0002]

2. Description of the Related Art Today, several drugs such as clofibrate-related preparations have been proposed as therapeutic / preventive agents for atherosclerotic diseases or hyperlipidemia, which are one of the so-called typical adult diseases. In terms of pharmacological effects, side effects, etc., it is difficult to say that these are always sufficiently satisfactory, and there is a growing demand for more effective drugs. On the other hand, it is said that about 35% of blood cholesterol, which can be a direct cause of these diseases, is absorbed from food, and that various nutrients in food have a deep relationship with increase and decrease of blood lipid. So-called dietary treatments for improving medical conditions by ingesting therapeutic foods for a long time and changing eating habits are widely performed. This can be said to be the most preferable therapy because it is possible at home without worrying about side effects. Polysaccharides obtained by culturing certain microorganisms (for example, JP-A-57-29292) and dietary fiber obtained from corn fiber (for example, JP-A- 57-3
6947) has been attempted to be added to food and drink as a food material.

[0002]

However, since it is extremely complicated and difficult to separate and collect the active ingredient from the medium or the raw material, it is impossible to provide these to the market at a low price. Therefore, the present invention does not require steps such as troublesome separation, collection, and washing, and it is possible to easily and appropriately ingest an appropriate amount by adding the microorganism cell culture alone or to various foods and drinks. It is intended to provide a food and drink containing a cholesterol lowering agent. That is, the present inventors, various microorganisms belonging to the genus Streptococcus, dairy raw materials, carbohydrate raw materials, soybean raw materials or cereals, proliferate well in various edible media mainly using cereals,
The viable and dead cells can effectively lower blood cholesterol and triglyceride levels, and the origin of these cells is so-called intestinal bacteria, which are substantially non-toxic orally. And found that the present invention was completed.

Hereinafter, microorganisms, culture media, production steps of the agent, the form of use of the agent, pharmacological effects and acute toxicity involved in the production of the cholesterol-lowering agent according to the present invention will be described in detail. Microorganisms In the present invention, various microorganisms belonging to the genus Streptococcus may be used, among them, Streptococcus faecium, Streptococcus faecalis, Streptococcus bovis, Streptococcus ebium, Streptococcus durans, Streptococcus salivarius, Streptococcus, Streptococcus. , Streptococcus, Ikuinus, etc. may be mentioned as suitable examples. Further, specific strains particularly useful in the present invention are shown below together with the accession numbers of the microtechnical laboratories.

[Table 1]

[0004] In terms of microbiological properties mycological properties, microorganisms used in the present invention has the same properties as those indicated by the known respective documents for the same classification bacteria. That is, the following documents are referred to for the mycological properties and culture conditions of the microorganism of the present invention. 1) Bergey's Manual of Determinative Bacteriology,
8th ed., 490-509 (1974) 2) Int. J. Syst. Bact. 16 114 (1966) 3) Microbiol. Immunol. 25 (3), 257-269 (1981) 4) J. Clin. Pathol 33 53-57 (1980) 5) J. General Microbiol., 128 713-720 (1982) 6) Applied Microbiol., 23 (6) 1131-1139 (1972) The summary of mycological properties is shown below.

[Table 2]

[0005] The main raw material for edible medium which can be used for producing the cholesterol-lowering agent according to the medium present invention, whole milk, skim milk, skim milk, whey or milk material, such as enzyme-treated proteins contained in these, , Honey, molasses and other sugar raw materials, soy milk,
Examples include bean-based raw materials such as soymilk whey, and cereals such as wheat flour and rice flour. The medium concentration is 0.01 to 5%, more preferably 0.1 to 1.0%. Cultivation of bacterial cells The conventional method for culturing lactic acid bacteria in producing fermented milk may be used. That is, first, the medium prepared as described above was heated at 110 ° C for 10
Heat sterilize for ~ 30 minutes. After cooling to about 40 ° C, Streptococcus microorganism cells are inoculated at about 10 ⁵ cells / ml, and aerobically statically cultured at 37 to 40 ° C. However, as described above, since various edible media can be used, the culture time may be different from usual. For example, in a medium prepared with skim milk powder and water, it takes 11 hours or more for the bacterial cell concentration to reach approximately 10 ⁸ cells / ml, as shown in the experimental examples described below, whereas the tryptic case in tap water, In a liquid medium containing yeast extract and tryptose, the same cell concentration was obtained within 6 hours.

Pharmacological effect As shown in the following experimental examples, the cholesterol-lowering agent of the present invention extremely effectively lowers blood cholesterol level and triglyceride level, and the bacterial cells as its active ingredient are edible medium. Since it is cultivated in a normal medium, it is not necessary to completely wash and remove non-edible components such as heavy metals that are usually mixed in the medium, and the culture is simply subjected to treatments such as freeze-drying and spray-drying. As described above, it is possible to freely add various foods. Therefore, including arteriosclerosis, hyperlipidemia, hyperlipoproteinemia, xanthomatosis,
It makes it extremely easy and long-term at home to treat or prevent diseases such as cholelithiasis, hypertension and diabetes. The dose of the agent of the present invention is usually 10 ⁶
-10 ¹³ pieces / kg body weight / day, more preferably 10 ⁸ -1
It is about 0 ¹¹ pieces / kg body weight / day.

Acute toxicity As shown in the experimental examples below, the LD ₅₀ value of the agent of the present invention is 8.9 × 10 ^{8 to} 1.3 × 10 ¹⁰ cells / cell in the case of viable cells.
In the case of mice (intraperitoneal administration) and those composed of dead cells, the number is 6 × 10 ¹³ cells / mouse (intraperitoneal administration) or more for any of the bacteria. In the case of oral administration, both live and dead cells are substantially non-toxic. Destruction treatment of microbial cells It is desirable that the microbial cells be subjected to a destructive treatment by an autoclave or ultrasonic waves so that the active ingredients in the microbial cells act more effectively. Next, an example is shown.

Example 1 The above microorganisms were inoculated into 5 liters of a medium consisting of 5% nonfat dry milk, and aerobically statically cultured at 37 ° C. for 10 hours to obtain a viable cell count of 5
A culture solution of × 10 ⁷ / ml was prepared, and the obtained culture solution was
After autoclaving at 15 ° C. for 10 minutes, a suspension of disrupted cells is obtained. Example 2 A culture solution obtained in the same manner as in Example 1 was subjected to ultrasonic disruption treatment at 15 KC for 60 minutes to obtain a suspension of disrupted cells.

Concentration of bacterial cells The solid content of the edible medium according to the present invention is 0.
Within the range of from 01 to 5%, for example, by adding to a food or drink an amount equivalent to a predetermined number of bacterial cell cultures obtained in a medium composed of a milk material, the flavor of the food or drink itself is significantly impaired. There are cases. Therefore, depending on the type of food or drink to be added, it may be necessary to concentrate the cells in the culture.
Here, the method for concentrating cells by centrifugation can be the most preferable method because the operation is simple and economical. 700-1000 × g of unbroken cells,
Since the precipitate is obtained in 5 to 10 minutes, it is concentrated to 1/10 to 1/100 under the centrifugal conditions in this range. Even when the cell culture is dried to a final water content of about 1 to 3%, it may be desirable to carry out centrifugation as a pretreatment.

Drying of Culture The culture of bacterial cells obtained by the above method is dried by appropriate means. Drying method is freeze drying, spray drying, Foam-
Mat drying, centrifugal thin film drying method, foam drying method, etc. are selected according to the use form. An example is shown below.

Drying Example 1 Microorganisms are cultured for 8 hours according to the method of Production Example 10, and the cells are collected from the culture by centrifugation, suspended in 10% skim milk, and then dispensed into ampoules ( Bacterial concentration 10
⁹ / ml). The ampoule is cooled to −30 ° C. and frozen, and the ampoule is vacuum-dried while being frozen and then sealed. Long-term storage of viable bacteria is possible at about 5 ° C. Water content is 2%.

Drying Example 2 A culture solution obtained by culturing microorganisms for 10 hours in accordance with the method of Production Example 2 was neutralized with sodium hydrogen carbonate, and sterilized by heating at 110 ° C. for 10 minutes. Concentrate briefly to a solids content of 50%. This is 57
Emulsified with a 100 kg / cm ² single-stage homogenizer at
Fine porous glass blowing machine with N ₂ gas to uniformly blow spongy and dried and then cooled to 13 ° C., to obtain a dry concentrate of multi foam quality. When this is crushed, it is extremely excellent in water reconstitution, and thus becomes a beverage additive that can be easily added to tea, coffee and the like to be dissolved.

Form of Use The cholesterol-lowering agent according to the present invention comprises cells obtained from an edible medium, and various treatment steps can be carried out relatively easily as described above. It can take various forms. That is, it is possible to ingest the cell culture as it is, or as a food or drink simply by adding an additive such as a seasoning. It is also possible to add it to various foods and drinks in the state after performing. For example, when obtaining a lactic acid beverage, fermented milk or a soft drink or other food characterized by sourness, the culture may be added as it is, but tea, seasonings,
For other foods that do not impair the flavor of itself, the cells are concentrated to remove as much of the components of the culture medium as possible and the specified dose can be consumed, and preserved in tea, etc. The use form of the agent of the present invention is selected depending on the properties or characteristics of each food itself to be added and the dose to be taken of the agent, for example, the necessity of performing a drying treatment for convenience.

[0014]

EXAMPLES Next, examples of production, pharmacological effects, acute toxicity and food blending examples of the cholesterol lowering agent according to the present invention will be shown by examples. Example 1 (Production Example) An example of production of the cholesterol lowering agent according to the present invention is shown below. Production Example 1 110% skim milk powder adjusted to a concentration of 10% by weight was prepared.
Heat sterilized at 10 ° C for 10 minutes, Streptococcus faecalis ADV9001, Streptococcus faecium ADV1009, Streptococcus durans A
DV3001, Streptococcus ebium AD20
03 alone, the viable cell count concentration was about 10 ⁵ cells / ml
And cultured statically at 37 ° C. FIGS. 1 and 2 show how the viable cell count concentration and pH change. For all 4 strains, the maximum viable cell concentration was approximately 10 ⁸ cells / ml,
It showed that it could grow well in skim milk powder. In addition, those inoculated with Streptococcus faecalis ADV9001 decreased the pH to 4.2, and those inoculated with Streptococcus faecium ADV1009 reduced the pH to pH 4.4, causing curdling. The pH of the inoculated product did not decrease so much and it did not curd even after one week of culture. Next, the bacterial cell culture solution cultured for 10 hours by the above method was subjected to 3000 rpm for 15 minutes.
The cells were condensed to 1/10 by centrifugation at m and spray-dried to obtain granular white powder.

Production Example 2 Commercially available milk was heat-sterilized at 110 ° C. for 10 minutes, and the above four strains were inoculated and cultured as in Production Example 1. Table 3 shows the viable cell count concentration and pH after 24 hours. The four strains grew well in commercial milk. The curd had the same results as in Production Example 1. Next, a culture solution of bacterial cells cultured for 10 hours by the above method was treated in the same manner as in Production Example 1 to obtain a white powder.

Production Example 3 Commercially available soybeans were immersed in water overnight, crushed, added with 8 times the weight of soybean weight before immersion, heated at 80 ° C. for 10 minutes, and filtered with a cloth to obtain soymilk. This was heat-sterilized at 120 ° C. for 10 minutes, and the four strains of the previous example were inoculated and cultured in the same manner as in the previous example. 24
Table 3 shows the viable cell count concentration and the pH after time. All four shares
It grew very well and within 24 hours the soy milk had solidified.

Production Example 4 One volume of commercially available molasses (manufactured by Dainippon Sugar Co., Ltd.) and 9 volumes of water were mixed, adjusted to pH 7 with 1N NaOH, and sterilized by heating at 110 ° C. for 10 minutes. Inoculate 4 strains of the previous example in the same manner as the previous example,
Cultured. Table 3 shows the viable cell count concentration and pH after 24 hours. All four strains grew, but in particular, Streptococcus
E. faecalis ADV9001 and Streptococcus faecium ADV1009 grew well.

Production Example 5 Four strains of the preceding example were inoculated into a liquid medium prepared as described below in the same manner as in the preceding example, and were cultured aerobically at 37 ° C., and all the strains grew well. In about 6 hours, a viable cell concentration of 5 × 10 ⁷ cells / ml or more was obtained. Next, the culture obtained by culturing for 8 hours by the above method is centrifuged (4000 rpm).
pm, 10 minutes) to concentrate the cells to 1/100, and freeze-dried to obtain a solid.

Composition of liquid culture medium 1 liter of distilled water 25 g of tryptic case 15 g of yeast extract 10 g of tryptose

Production Example 6 11 of skim milk powder adjusted to have a weight concentration of 0.8%
The solution was sterilized by heating at 0 ° C. for 10 minutes, inoculated with the four strains of the preceding example so that the viable cell concentration was about 10 ⁵ cells / ml, and cultured at 37 ° C. in static. Table 3 shows the viable cell count concentration and pH after 24 hours.

Production Example 7 Commercially available milk was diluted 10-fold, heat-sterilized at 110 ° C. for 10 minutes, and inoculated and cultured with the four strains of the previous example as described above. 24
Table 3 shows the viable cell count concentration and the pH after time. Next, the culture obtained by culturing for 10 hours by the above method was centrifuged (4000 rpm, 10 minutes) to concentrate the cells to 1/100, and then a freeze-dried product was obtained.

Production Example 8 Soymilk having a solid content of 0.6% was heat-sterilized at 120 ° C. for 10 minutes, and the four strains of the previous example were inoculated and cultured in the same manner as in the previous example. 2
Table 3 shows the viable cell count concentration and pH after 4 hours. According to the above method, the culture solution obtained after 10 hours was centrifuged in the same manner as in the previous example, and this was spray-dried to obtain a granular powder.

Production Example 9 1 volume of commercially available molasses (manufactured by Dainippon Sugar Co., Ltd.) and 11 volumes of water were mixed, adjusted to pH 7 with 1N NaOH, and then heated to 110 ° C.
For 10 minutes. In the same manner as in the previous example,
Inoculated and cultured. Table 3 shows the viable cell count concentration and pH after 24 hours. Next, the culture solution obtained by culturing for 10 hours by the above method was subjected to an ultrasonic disruption treatment (15 KC, 60 minutes) to obtain a disrupted cell suspension.

Production Example 10 Four strains of the preceding example were inoculated into a liquid medium prepared as described below in the same manner as in the preceding example, and were cultured aerobically at 37 ° C., and all the strains grew well. In about 6 hours, a viable cell concentration of 2 × 10 ⁷ cells / ml or more was obtained. The culture solution obtained by culturing for 8 hours by the above method was heated at 115 ° C. in an autoclave.
, And then centrifuged (4000 rpm, 15 minutes) to obtain a concentrated freeze-dried portion containing the disrupted cells. Composition of liquid medium 1 liter of distilled water Trypticase 6g Yeast extract 3g Tryptose 2g

[Table 3]

Example 2 (Pharmacological Action) Hereinafter, experimental examples relating to the pharmacological effect and acute toxicity of the cholesterol-lowering agent according to the present invention will be described. EXPERIMENTAL EXAMPLE 1 The culture of Production Example 10 was freeze-dried without subjecting it to bacterial cell destruction, and was lyophilized into normal and sterile mice (male 16 weeks old, average body weight 19 g; 10 mice per group), normal rats (male 16 Week age, average body weight 232 g;
They were added to a diet equivalent to ¹⁰ rats and allowed to ingest freely, and then reared for 4 weeks on the diet alone. Then, arterial blood was collected from the inferior aorta of these mice and rats and centrifuged to obtain a serum sample, which was used as a choles kit (trade name; manufactured by Kanto Chemical Co., Inc., Zurk
owski method) and glyceride TG Wako (trade name; manufactured by Wako Pure Chemical Industries, acetylacetone extraction method) were used to measure cholesterol and triglyceride levels in serum samples. The composition (% by weight) of the diet is as follows. Diet composition Casein 20 Soybean oil 10 Wheat starch 61 Mineral 4 Vitamin mixture 2 Filter paper powder 3 The results obtained are shown in Table 4. In the table, "cholesterol load" or "fructose load" indicates the case where a feed obtained by further adding 1% cholesterol to the feed or a feed obtained by completely replacing wheat starch with fructose is used. Is the reduction rate relative to the control.

EXPERIMENTAL EXAMPLE 2 The freeze-dried product obtained in Production Example 10 was used for normal rats (male 16 weeks old, average body weight 235 g; 10 mice per group), normal and sterile mice (male 16 weeks old, average body weight 18 g; Each group 1
0) were orally ingested daily for 4 weeks at an amount equivalent to 10 ¹⁰ dead cells. Next, arterial blood was collected from the lower aorta of these rats, and cholesterol and triglyceride levels were measured in the same manner as in Experimental Example 1. Diet was also given in the same manner as in Experimental Example 1. Table 5 shows the results.

EXPERIMENTAL EXAMPLE 3 The culture of Production Example 10 was subjected to continuous centrifugation at 12,000 rpm to collect the cells, and the cells were washed with physiological saline.
The suspension was suspended in physiological saline to obtain a bacterial solution (50 ml, 10 ¹¹ / ml).
g; 15 mice per group), normal and sterile mice (male, 18 weeks old,
Average weight 31 g; 10 animals per group) for 12 weeks, 10 ¹¹ /
It was orally administered daily on a daily basis, and the decrease rates of serum cholesterol and triglyceride levels were measured in the same manner as described above. Table 6 shows the results. In the table, “cholesterol load” or “fructose load” indicates the case where a feed obtained by further adding 1% cholesterol to the feed or a feed obtained by completely replacing wheat starch with fructose is used. The rate of decrease is shown with the administration group as a control.

EXPERIMENTAL EXAMPLE 4 The suspension of living bacterial cells in the saline solution of Experimental Example 3 was washed twice with physiological saline, and then suspended in physiological saline (aqueous 0.85% NaCl solution) to obtain 50 ml of a bacterial solution. (10 ¹¹ / ml)
Is heated at 115 ° C. for 10 minutes to obtain a cell suspension. This was administered to a normal rat (male 18 weeks old, average weight 246 g;
5) and sterile mice (male 18 weeks old, average weight 30g;
10 ¹¹ animals were orally administered to each group for 12 and 8 weeks after oral administration, and the serum cholesterol and triglyceride reduction rates were measured by the same method as described above. Table 7 shows the results.

[0029]

[Table 4]

[0030]

[Table 5]

[0031]

[Table 6]

[0032]

[Table 7]

Experimental Example 5 S.P. Fesium ADV10
No. 09 was cultured, and the culture was freeze-dried by the same method as described above.
33 g, bred on a cholesterol-loaded diet, 10 animals per group) were added to the diet of Table 2 in an amount equivalent to 10 ¹¹ killed cells for 2 weeks, and continuously orally ingested.
Next, the cholesterol level in the arterial blood of these rats was measured in the same manner as in Experimental Examples 1 to 4. The results are shown in FIG. The blood cholesterol level and triglyceride level lowering rate of the rat were changed depending on the culture time.

Experimental Example 6 ICR mouse (male 6 weeks old, average body weight 30.0 ± 0.5
g) and the physiological saline 0.5 used in Experimental Example 3 was used.
9 × 10 ⁹ , 9 × 10 9
Intraperitoneal administration of a considerable amount to ^{8, 9 × 10 7} bacterial cells in three stages (10 mice in each group) was performed, and the survival of the mice was observed for 14 days.

[Outside 1] Table 8 shows the LD ₅₀ value (number of cells / mouse) calculated according to
Shown in In the case of dead cells, LD
_{The 50} value is 6 × 10 ¹³ cells / mouse or more (intraperitoneal administration) and in any case of oral administration, of course,
It was non-toxic at all.

[Table 8]

Example 3 The cholesterol-lowering agent according to the present invention can be taken alone or as it is by simply adding additives such as seasonings and flavors. It is also possible to use it and its range of use is very wide. Examples of food formulations using the same are shown below. Food formulation example 1 (powder soup) Raw material formulation (g) Cooked bean powder 3600 Wheat flour 135 Dry yeast powder 90 Dry onion 90 Salt 11 White pepper 91 (oz) MSG 12 Powder Laurel leaf 3 Powder of production example 2 300

Food Mixing Example 2 (Ochazuke Nori) Raw Material Mixing (g) Arare 2.5 Nori 0.75 Seasoning Granules 3.3 Freeze-dried Product of Production Example 0.3

Food Formulation Example 3 (curry roux) Raw material formulation (g) Beef tallow 40 Sugar ester 0.5 Wheat flour (thin strength) 31.7 Salt 10 Sugar 2 MSG 1 Skim milk powder 1.5 Curry powder 6.2 Onion powder 1. 6 Caramel 0.5 Adipol beef (powder) 5 Foam dried product of Production Example 6 0.8

Food Formulation Example 4 (Hamburger) Raw Material Formulation (g) Minced Meat 20 Vegetable Protein Meat 10 Onions 40 Eggs 10 Bread 10 Baked Wheat Flour 3 Salt 1 Pepper 0.4 Margarine 5 MSG 0.8 Foam Dried Product of Production Example 9 0.5

Food Formulation Example 5 (Fruit Milk Beverage ) Raw Material Formulation (g) Skim Milk 70 Fruit Juice 5 Citric Acid 0.3 Sugar 10 Pigment 0.1 Flavor 0.2 Stabilizer 0.4 Production Example 1 Culture 14 An example of the cholesterol-lowering agent according to the present invention, which is one of the easiest modes of use at home and which can best serve the purpose of the present invention, is a beverage additive. As described in the example of the step of drying the cell culture, the production method is either a method of condensing the culture and drying the foam, or a method of Form-mat dryin.
It is characterized by drying by the g method. The powder obtained by this method was used for the cell count of 5 × 10 ^{7 to} 5 × 10 ⁹ cells per person.
Equivalent amount (5% for the additive processed according to Dry Example 2)
When added to tea, coffee, yogurt, juice, etc., they are quickly mixed or dissolved, and do not impair the flavor or appearance of the beverage. Therefore, the use of this drug in these forms does not significantly alter daily eating habits,
This makes it possible to easily perform long-term treatment and prevention of arteriosclerosis and the like. In addition, as shown below, a diet can be more effectively administered by adding an appropriate amount to a therapeutic diet every time.

Example of Diet for Treating Hyperlipoproteinemia Breakfast Coffee or black tea (additional amount of foam dried product of 3 × 10 ⁹ equivalent amount) Bread (rye and wheat) 30 g Margarine 10 g Cottage cheese 60 g

Snack 150 g of skim milk yogurt (80 mg of spray-dried product of Production Example 6 added) Knecke 8 g Margarine 5 g

Lunch Tonkatsu pork 100 g oil 5 g carrot dish carrot 150 g margarine 5 g (24 mg of the spray dried product of Production Example 8 is added) potato 60 g pear 100 g

Dinner Black tea (Add the same amount of dried foam of the same agent as the bacterial count of 2 × 10 ⁹ pieces) Whole grain bread 50 g Margarine 10 g Ham 40 g Tomato salad Tomato 100 g Onion 10 g Oil 3 g

[Brief description of the drawings]

FIG.

FIG. 2 is a diagram showing changes in the viable cell count in a medium over time,
FIG. 2 is a diagram showing the change over time in the pH of a culture. A in FIG. Durance ADV3001, B. Ebium AD2003, C is S.P. Fesium ADV100
9, D is S.D. Fecaris ADV9001.

FIG. 3 is a graph showing changes in blood cholesterol and triglyceride lowering rates of hyperlipidemic rats depending on the culture time of bacterial cells. Solid line A indicates the cholesterol lowering rate, and broken line B indicates
The triglyceride reduction rate is shown.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display area A61K 35/74 ADN A61K 35/74 ADN

Claims (1)

[Claims] 1. A milk of one or more kinds selected from the group consisting of Streptococcus faecium, Streptococcus faecalis, Streptococcus evium, Streptococcus salivarius, Streptococcus durans, Streptococcus mitis and Streptococcus ikuinus. The dried concentrate obtained by culturing in an edible medium containing one or two kinds selected from the group consisting of raw materials and legume raw materials as the main raw material is concentrated and then dried to obtain cholesterol. Foods and drinks containing as a reduced active ingredient.