JPH08764B2 - Cosmetics containing immobilized enzyme - Google Patents

Description

The present invention relates to cosmetics, and more particularly, to at least amylase, protease, or lipase immobilized on hydroxyapatite together with a binder so as to strongly adsorb to hydroxyapatite. The present invention relates to cosmetics containing one type of immobilized enzyme.

(Conventional Technology) The skin constantly secretes sebum from the sebaceous glands and sweat from the sweat glands,
Renews skin keratin due to metabolism. There are individual differences in the amount of sebum, sweat secretion, and keratin renewal. Excessive sebum secretion causes a so-called greasy phenomenon, which not only worsens the paste of cosmetics but also easily causes seborrhea, acne and the like. Further, the hardening of skin keratin due to ultraviolet rays causes the elasticity of the skin to be lost and reduces the water retention of the skin. Thus, skin function always requires maintaining proper skin condition. Various cosmetics are used to keep the skin normal, to smoothly perform the skin function, and to give an aesthetic appearance. For example, astringent lotion suppresses excess oil in the skin and causes the skin to converge, and alkaline lotion to wash away dirt and secreted oils gives the skin a moist feeling and vanishing cream to prevent chapping. Alternatively, an emulsion is used to harden skin keratin due to poor sebum secretion, sunburn, etc. Thus, in order to remove sebum and treat hardened keratin, each drug is added to each cosmetic product and used. That is, keratin-dissolving agents such as salicylic acid, sulfur and urea are used for removing keratin, or soap and alkaline substances are added to cosmetics for removing sebum. Since the cause of dirt on the skin is secreted fats, proteins, and sugars, the idea of using enzymes that decompose these in cosmetics has existed for a long time, but enzymes are used in various drugs and pH. Since it is unstable to temperature, it is inactivated quickly when it is mixed in cosmetics as it is, and it is difficult to expect its effect during storage or during production, and it is difficult to expect its effect. Didn't.

(Problems to be Solved by the Invention) The present invention is to fix carbohydrates, proteins, and lipids that cause skin stains to an enzyme amylase, protease, or lipase, which is immobilized on hydroxyapatite and then incorporated into cosmetics. In addition, instead of immobilizing these enzymes on an insoluble carrier, by encapsulating the enzymes in a cosmetic product, any commercially available enzyme can be incorporated into the cosmetic product, and a wide range of temperature and pH can be obtained. By providing an enzyme-containing cosmetic that is stable over a long period of time and can be stored for a long time, a cosmetic that is safe for the human body can be obtained without using keratolytic agents, alkalis and other agents that have been used to remove sebum and keratin. Is.

(Means for solving problems) Amylase, an enzyme that decomposes carbohydrates and glycogen, is α-
Amylase, β-amylase, including saccharified amylase, those extracted from swine pancreas, barley germ, sweet potato, barley, Bacillus, Aspergillus, etc. are commercially available, and enzymes that decompose proteins are pepsin, trypsin, chymotrypsin. , Animal systems such as calyculene, plant systems such as papain, promeline, physin, and bacterial proteases such as Streptomyces and mold are commercially available. Lipases that decompose lipids are animals such as triacetin, tristearin, and triolein. System, wheat germ, castor seeds, plant systems such as extracts from rapeseed, candida, molds such as extracts from Kumonosukai, bacterial lipases are commercially available. Any of the enzymes can be used. As the insoluble carrier, conventionally, titanium oxide, kaolinite, clay (bentonite), calcium phosphate, zinc oxide, inorganic substances such as zeolite, ion exchange resin, polyamino acid, carboxymethyl cellulose, p-aminobenzene cellulose, cellulose, granular silicon aminosilane derivative, etc. However, the hydroxyapatite used in the present application is easily produced from slaked lime and phosphoric acid by a conventional method, has a composition of Ca ₁₀ (PO ₄ ) ₆ (PH) ₂ , and is a human bone component. Therefore, even if it is absorbed by the human body, it is harmless, unlike other inorganic substances, and has a stronger protein adsorption force than other inorganic substances. It is desired that the size of the hydroxyapatite particles is 9 μ or more and 50 μ or less. This is because fine particles of 9 μ or less are permeated through the skin, and particles of 50 μ or more give the skin a rough feeling. To immobilize the enzyme on hydroxyapatite, one of the binders selected from hexamethylenediamine, glutaraldehyde, hexamethylenediisocyanate, etc. is used.

The enzyme, hydroxyapatite and binder to be used can be arbitrarily selected according to the type of cosmetics used, and the immobilization can be carried out by the existing immobilization method. That is, an enzyme, a binder, and hydroxyapatite are added to a buffer solution having a pH of about 7, and the mixture is stirred at a low temperature (about 5 ° C.) for several hours to obtain a synthetic product. The insoluble enzyme thus obtained is thoroughly washed with a buffer solution and then with cold water, and dried by freeze-drying.

The same effect can be obtained by using microcapsules encapsulating the enzyme instead of immobilizing the enzyme. Microencapsulation of enzymes can also be performed by existing methods.
That is, an enzyme solution is added to an organic solution such as benzene or ether containing nylon, polystyrene, polyisocyanate, etc. to completely emulsify, and this emulsion is dispersed in a protective colloid solution containing a nonionic surfactant or gelatin. Get by

The thus-obtained immobilized enzyme or microcapsule has long-term enzymatic activity and can be applied to the human body by long-term incorporation into cosmetic materials according to the purpose, such as lotion, cream, lotion and other purposes. Safe and efficient, keratin, lipid,
It is possible to provide a cosmetic product that decomposes sugars and promotes skin metabolism.

(Action) Since the enzyme is immobilized or microencapsulated, it shows stable enzyme activity over a wide range of temperature and pH. Immobilization increases the Michaelis constant and accelerates the decomposition rate, so it is short-term. It decomposes keratin and is safe for the human body.

(Examples) The present invention will be specifically described below with reference to Examples.

The enzymes used in this experiment were proteolytic bioburase, wheat germ lipase, and barley amylase, and their immobilization was performed as follows. Finely powdered hydroxyapatite was suspended in an ice-cooled buffer solution of pH 7.5, the same buffer solution containing hexamethylenediamine was added with stirring, and after a few minutes, an enzyme dissolved in the same buffer solution was added, and then at 4 ° C., After stirring overnight, the solid obtained by centrifugation was thoroughly washed with a buffer solution and then with cold water, and freeze-dried. In each experiment, the components other than the immobilized enzyme were added to the surfactant heated to 50 ° C while stirring vigorously, and the immobilized enzyme or an aqueous solution in which the immobilized enzyme was suspended was added and stirred. While allowing to cool to room temperature.

Example 1. Oily ointment-type foundation wt% Immobilized protease 10 Isopropyl palmitate 15 Lanolin alcohol 5 Lanolin acetate 8 Microcrystalline wax 7 Liquid paraffin 24 Candelilla wax 0.5 Powdered hydroxyapatite 30 Preservative, antioxidant Proper amount Example 2. Oily stick Type Foundation wt% Immobilized lipase, immobilized protease, immobilized amylase 15 Zinc white 30 Liquid paraffin 17 Isopropyl myristate 15 Liquid lanolin 4.5 Microcrystalline wax 4.5 Ceresin 10 Carnauba wax 2 Sorbitan sesquioleate 1 Preservative, proper amount Fragrance Appropriate amount Example 3. Emulsion foundation wt% Immobilized protease, immobilized lipase 12 Stearic acid 2.4 Propylene glycol monostearate 2 Cetostearyl alcohol 0.2 Lanolin 2 Liquid paraffin 3 Isopropyl myristate 8.5 Water balance Example 4. Targeting a cosmetic composition having the same composition as in Example 1 except that bentonite-immobilizing enzyme was used instead of hydroxyapatite-immobilizing enzyme, the cosmetic composition of Example 1 A comparative experiment with

20 inspectors used it once a day for 3 consecutive days and obtained the following results.

 Apatite Target group (People) (People) Skin was shiny 20 18 Smooth 20 16 Skin was moist 20 16 Irritating 0 2 Color and odor were not observed with time .

(Effect of the invention) Since the enzyme is immobilized, it exhibits enzyme activity over a wide range of temperature and pH, and the action time of the enzyme is shortened to effectively act on the removal of dirt on the skin, while hiding the insoluble carrier itself. The force helps improve the hiding power of the cosmetics themselves.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kiminori Atsumi 2-11-10 Tsukiji, Chuo-ku, Tokyo (Tsukiji Central Building) Sangi Co., Ltd. (72) Inoue Souki, 2 Tsukiji, Chuo-ku, Tokyo No. 11-10 (Tsukiji Central Building) Sangi Co., Ltd. (72) Inventor Keijiro Fujita 2-11-10 Tsukiji, Chuo-ku, Tokyo (Tsukiji Central Building) Sangi Co., Ltd. (72) Inventor Tsutomu Ishizaki Tokyo 2-11-10 Tsukiji, Chuo-ku, Tokyo (Tsukiji Central Building) Sangi Co., Ltd. (56) Reference JP 61-268611 (JP, A) JP 61-257906 (JP, A) JP 57-73098 (JP, A) JP-A-57-11905 (JP, A) JP-B 43-11290 (JP, B1) JP-B 49-21047 (JP, B1) JP 111955 (JP, C1) US Patent 3654083 (US, A) Kozaki Road "Enzyme Handbook", supervised by Yu Chijin Shokan Co., Ltd. 477 Fragrance Journal NO. 38 P. 51-53, NO. 57 P. 61-64, NO. 57 P. 66

Claims (1)

[Claims] 1. A binder of amylase, protease or lipase selected from hexamethylenediamine, glutaraldehyde, or hexamethylene diisocyanate.
In addition, a cosmetic product containing an immobilized enzyme containing at least one of the immobilized enzymes strongly adsorbed to hydroxyapatite.