JPH086204A - Optical recording medium, optical recording method and information reproducing method - Google Patents

Abstract

PURPOSE:To make it possible to reproduce information with high quality and to produce an optical recording medium at a low cost by having a recording layer contg. a nucleic acid and dyestuff which emits fluorescence by photoirradiation in co-presence of the nucleic acid on a substrate. CONSTITUTION:The recording layer 2 contg. the nucleic acid and the dyestuff which emits the fluorescence by photoirradiation in co-presence of the nucleic acid is laminated on the substrate 1. The dyestuff described above is not particularly limited, insofar as the dyestuff emits fluorescence when subjected to the photoirradiation in co-presence of the nucleic acid. Such dyestuff includes, for example, cumarin dyestuff, such as rhodamine 6G and rhodamine B, as well as acridine dyestuff, pyrium dyestuff, etc. While the part not irradiated with light exhibits the high fluorescence, the part irradiated with the light hardly exhibits the fluorescence as the dyestuff is dissolved, when the recording layer 2 formed by contg. the nucleic acid and the dyestuff which emits the fluorescence by photoirradiation in co-presence of the nucleic acid is selectively irradiated with the light. The information is reproduced at high S/N by detecting the presence or absence of the fluorescence between both.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an optical recording medium, a method of recording information on the optical recording medium, and a method of reproducing information from the optical recording medium.

[0002]

2. Description of the Related Art In general, an optical recording medium (for example, an optical disk or an optical card) has a small recording layer provided on a substrate, which is optically detectable along a spiral, concentric, or striped track. , Information is recorded at high density by forming pits of about 1 μm.

As a method of recording information, for example, in a certain heat mode recording method, when a laser beam focused on the surface of the recording layer is scanned, laser energy is absorbed in the recording layer and converted into heat. Evaporation, decomposition, etc. occur in the laser light irradiation portion of the recording layer, and as a result, optically detectable concave pits are formed and information is recorded.

Further, in another heat mode recording method,
Absorption of laser energy in the recording layer forms a pit having a density difference that can be optically detected at that portion, and information is recorded.

The pits thus recorded are reproduced by detecting the difference in reflectance between the non-pit portion and the pit portion by irradiating the reproducing light.

By the way, as a recording layer of an optical recording medium, there has been proposed a recording layer mainly using an inorganic substance such as a metal thin film such as an aluminum vapor deposition film, a chalcogenide type amorphous glass film such as a bismuth thin film or a tellurium oxide thin film. However, in recent years, an organic dye recording layer has been proposed which can be formed into a film by coating and can reduce the manufacturing cost of an optical recording medium.

However, since the organic dye recording layer generally has a lower reflectance than the metal recording layer, there is a problem that the quality of the reproduced signal is not sufficient.

[0008]

SUMMARY OF THE INVENTION The present invention has been made in view of the above problems, and an object of the present invention is to provide an optical recording medium capable of reproducing high quality information and manufactured at low cost. It is what

Another object of the present invention is to provide a method of recording information that can be reproduced at a high S / N ratio.

It is another object of the present invention to provide a method of reproducing information which enables a reproduced signal of high quality to be obtained.

[0011]

Means for Solving the Problems That is, the present inventors have conducted various studies on the above object, and as a result, a thin film containing a nucleic acid and a dye capable of emitting fluorescence by light irradiation in the coexistence with the nucleic acid. The non-irradiated part shows high fluorescence when selectively irradiated with light, whereas the light-irradiated part shows almost no fluorescence, probably because the dye is decomposed, and the presence or absence of fluorescence in both is detected. The present invention has been achieved by finding that information can be reproduced at a high S / N ratio, and the optical recording medium of the present invention can be used on a substrate in the presence of nucleic acid and coexistence with the nucleic acid. It is characterized by having a recording layer containing a dye that emits fluorescent light when irradiated with light.

In the optical recording method of the present invention, recording light is selectively applied to an optical recording medium having a recording layer containing a nucleic acid and a dye capable of emitting fluorescence upon irradiation with light in the presence of the nucleic acid. The feature is that information is recorded by irradiating the.

Further, the information reproducing method of the present invention comprises:
Having a recording layer containing a nucleic acid and a dye capable of emitting fluorescence upon irradiation with the first light in the coexistence with the nucleic acid, and selectively erasing the luminescent ability of the recording layer upon selective irradiation with the second light The information is reproduced by irradiating the optical recording medium on which the information has been recorded with the first light and detecting the contrast between the light emitting portion and the non-light emitting portion. .

Furthermore, the information reproducing method of the present invention comprises:
A recording layer containing a nucleic acid and a dye capable of emitting fluorescence by irradiation of a first light in the coexistence of the nucleic acid, and the recording layer is selectively made to emit a fluorescence by the selective irradiation of a second light. The information is reproduced by irradiating the optical recording medium on which the information is recorded with the first light and detecting the contrast between the light emitting portion and the non-light emitting portion.

Next, the present invention will be described in detail with reference to the drawings.

FIG. 1 is a schematic cross-sectional view showing one embodiment of the optical recording medium of the present invention, which shows a substrate 1 and a nucleic acid and a dye which emits fluorescence when irradiated with light in the coexistence with the nucleic acid. The recording layer 2 including it is laminated.

The dye used in the present invention includes
The dye is not particularly limited as long as it is a dye that emits fluorescence by being irradiated with light in the coexistence with a nucleic acid. For example, rhodamine 6G, rhodamine B, fluorescein, calcein blue, eosin, diethylaminomethylcoumarin, and Coumarin-based dyes such as 4-methylumperiferone, acridine-based dyes such as acridine red and acridine orange, proflavin, ethidium bromide, donomycin, actimicin, and pyrylium-based dyes.

The emission of fluorescence due to light irradiation in the coexistence of such a dye with a nucleic acid is considered to be due to the interaction between the dye and the nucleic acid, but the dye may interact with the double helix of the nucleic acid. Known forms include intercalation between nucleic acid base pairs and embedding in a groove of a double helix.

Among them, a dye that intercalates between nucleic acid base pairs, a so-called intercalator, is generally a compound on a plane having a spread of electrons, on the extension of stacking of nucleic acid base pairs, that is, on the axis of a double helix. At a distance similar to the distance between nucleic acid base pairs, it is oriented parallel to the nucleic acid base pairs. The optical absorption spectrum when intercalated in the double-stranded nucleic acid is shifted to the long wavelength side, the absorption intensity is decreased, or the fluorescence intensity is increased, also in the present invention, The use of dyes (intercalators) that intercalate with nucleic acids as dyes used in the recording layer, such as acridine dyes, proflavin, ethidium bromide, donomycin, actimicin, and pyrylium dyes, is highly informative.
This is preferable for reproducing at N ratio.

In particular, the pyrylium dye compound as represented by the above-mentioned general formula [I] intercalates in the double helix of nucleic acid, and its fluorescence intensity is in the state of the dye alone. It will be significantly increased compared to. In addition, the maximum absorption wavelength shifts to the long wavelength side due to the interaction with the double-stranded nucleic acid, and the Stokes shift is large, which makes it possible to separate the recording light and the reproduction light from each other, and further to detect the incident light during reproduction and The optical recording medium of the present invention, in which information can be reproduced by giving a reproduction signal having a high S / N ratio because the wavelengths of light can be separated, and the stability is remarkably improved by intercalating a dye with a nucleic acid. It is particularly preferably used for.

Although the reason why the dye having the structure represented by the above general formula [I] exhibits such a phenomenon in the coexistence with a nucleic acid is not clear, the pyrylium ring and the substituent bonded to the ring are
In the state of the dyes alone, each dye has a certain bond angle (for example, about 30 degrees in the case of aminothicanphenyl-substituted pyrylium salt), but the dyes are disjointed. It is considered that, due to the biological effect, the bond angle was suppressed and became linear, and a long resonance system was generated in the dye molecule. Furthermore, since the dye is oriented in a position parallel to the nucleic acid base pairs, it is considered that the fluorescence intensity of the dye thin film also increases.

Representative examples of the dye represented by the above general formula [I] are shown in Table 1 below.

[0023]

[Table 1]

[0024]

[Table 2]

[0025]

[Table 3]

[0026]

[Table 4]

[0027]

[Table 5]

[0028]

[Table 6]

[0029]

[Table 7]

[0030]

[Table 8]

[0031]

[Table 9]

[0032]

[Table 10]

[0033]

[Table 11]

[0034]

[Table 12]

[0035]

[Table 13]

[0036]

[Table 14]

[0037]

[Table 15]

[0038]

[Table 16]

[0039]

[Table 17]

[0040]

[Table 18]

[0041]

[Table 19]

Next, the nucleic acid used in the present invention is DN
A, RNA, a double-stranded nucleic acid, or an intramolecular double-stranded portion of a single-stranded nucleic acid can be used. Specifically, other than commercially available DNA such as calf thymus DNA and salmon sperm DNA,
It is also possible to use a polyadenylic acid-polythicidylic acid complex, a polyadenylic acid-polyuridylic acid complex, and the like.

The recording layer of the optical recording medium according to the present invention is formed on the substrate 1 by various methods such as a coating method and a vapor deposition method, and the coating method is preferable for reducing the manufacturing cost of the optical recording medium. Is the way. When the coating method is used, it can be formed by coating a solution in which a fluorescent dye and nucleic acid are dissolved or dispersed in a solvent on the substrate 1, and the solvent that can be used at the time of coating is the aforementioned fluorescent dye and nucleic acid. Is in a dispersed state or in a dissolved state, but generally, alcohol-based, ketone-based, ether-based, ester-based, halogenated hydrocarbon-based, aromatic-based,
Solvents such as aliphatic hydrocarbon type and fluorine type can be used. Further, each buffer solution such as water, acetonitrile, dimethylsulfoxide, phosphate buffer solution, acetate buffer solution can be used.

If desired, waxes, higher fatty acids, amides (eg oleylamide), binders (eg
Cellulose, polystyrene, a plasticizer, an oil agent, a dispersant, and other additives can be appropriately mixed to enhance the film-forming property and coating property of the recording layer.

Specific coating methods include dip coating method, spray coating method, spinner coating method, bead coating method, Mayer bar coating method, blade coating method, roller coating method and gravure coating method. It can be performed using a coating method.

The content of the fluorescent dye and the nucleic acid in the recording layer 2 is usually 30 to 100% by weight, preferably 50 to 100% by weight.
Is desirable. If it is less than 30% by weight, sufficient light absorption of the recording layer and sufficient fluorescence for the reproduction laser beam cannot be obtained. The ratio of nucleic acid to fluorescent dye is 1 base pair of nucleic acid.
One molecule per -40 base pairs, especially one dye per 10-20 base pairs is preferred for pyrylium dyes.

The thickness of the recording layer 2 is 100Å to 20 μm.
m, preferably 200Å to 1 μm. Incidentally, if it is possible to stably form a thin film having sufficient photofluorescence with respect to the recording laser light, it is preferable that the thin film be as thin as possible.

Further, in the optical recording medium of the present invention, as shown in FIG. 2, a protective layer 3 which is transparent to the recording and reproducing laser light can be provided on the recording layer 2 via, for example, an adhesive layer 4. . The protective layer 3 may be opaque when irradiated with light from the substrate 1 side.

Further, as shown in FIG. 3, an undercoat layer 5 may be provided between the substrate 1 and the recording layer 2.

Further, as shown in FIG. 4, it is possible to use both the protective layer 3 and the undercoat layer 5.

The undercoat layer has (a) improved adhesion, (b) water or gas barrier, (c) improved storage stability of the recording layer, (d) improved reflectance, and (e) solvent. It is provided for the purpose of protecting the substrate of (1) and (f) forming a pregroup. For the purpose of (a), various polymeric materials such as ionomer resins, polyamide resins, vinyl resins, natural polymers, silicones, liquid rubbers, or various substances such as silane coupling agents are used. For the purposes of (b) and (c), in addition to the above polymeric materials, inorganic compounds such as SiO ₂ and Mg can be used.
F ₂ , SiO, TiO ₂ , ZnO, TiN, SiN, etc.,
Metals or metalloids such as Zn, Cu, S, Ni, C
r, Ge, Se, Cd, Ag, Al or the like can be used. For the purpose of (d) a metal, eg Al, A
or an organic thin film having a metallic luster, such as a cyanine dye or a methine dye, can be used. For the purposes of (e) and (f), an ultraviolet curable resin, a thermosetting resin, a thermoplastic resin or the like can be used.
The thickness of the undercoat layer is 50Å to 100 μm, preferably 20
0Å to 30 μm is suitable.

The protective layer is provided for the purpose of protecting it from scratches, dust, dirt, etc., and improving the storage stability and reflectance of the recording layer. The same material as the undercoat layer is used as the material. can do. The thickness of the protective layer is 100 Å or more, preferably 1000 Å or more.

At this time, the dye of the present invention may be contained in the undercoat layer and / or the protective layer. In addition, a stabilizer, a dispersant, a flame retardant, a lubricant, an undercoat layer or a protective layer,
An antistatic agent, a surfactant, a plasticizer, etc. may be contained.

Further, as another constitution of the optical recording medium according to the present invention, the recording layer 2 using two recording mediums of the same constitution shown in FIGS. 1 to 4 (in some cases, one of them is used as a substrate only) is used. It may be a so-called air sandwich structure in which is placed inside and sealed, or a so-called close contact structure (bonding structure) in which the layers are adhered via the protective layer 3.

In the present invention, the substrate 1 is a glass plate, a transparent ceramic plate, PVC, polymethylmethacrylate,
A transparent plastic plate such as polycarbonate, polysulfone, or polyolefin resin can be used. In particular, polymethylmethacrylate, which has no optical birefringence and is hard and hard to be scratched, is preferable. The substrate is preferably an optically transparent material.

Tracking grooves, prepits, etc. may be formed on the surface of these substrates.

Further, as the protective substrate 3, polymethylmethacrylate, polycarbonate, polysulfone, vinyl chloride or the like can be used. Particularly, a thin protective substrate is strong against bending and adheres to printing ink or the like. A good and inexpensive polycarbonate is preferable.

Next, a method of recording / reproducing information on / from an optical recording medium containing a nucleic acid and a dye in the recording layer as shown in FIG. 1 will be described. The information recording method is the same as a normal recording method. Information can be recorded by irradiating the optical recording medium of FIG. 1 with recording light to form pits with or without a change in the shape of the recording layer. In particular, it is preferable to substantially erase the fluorescence emitting ability of the recording layer in the recording light irradiation part in order to reproduce information at a high S / N ratio. In the present invention, elimination of the fluorescence emission capability means, for example, decomposition (decolorization) of organic dye or decomposition of nucleic acid in the recording layer of the recording light irradiation part. That is, the information recorded by erasing the fluorescence emission capability of such a recording section is recorded in the fluorescence emission section (information not recorded, for example, when reproduced by using as the reproduction light such that the dye emits light in the presence of nucleic acid. The information can be reproduced by detecting the contrast between the recording portion) and the non-light emitting portion (information recording portion), and the information can be reproduced at a high S / N ratio.

The recording light that can substantially erase the fluorescence emission ability of the recording layer varies depending on the types of nucleic acids and dyes contained in the recording layer, so it cannot be determined unconditionally, but the maximum absorption of the recording layer is obtained. Laser light having an oscillation wavelength near the wavelength is preferable because it can efficiently decompose the dye in the recording layer by photothermal conversion in the recording layer.

Next, FIG. 5 is a schematic sectional view of another embodiment of the optical recording medium according to the present invention. In FIG. 5, 11 is a substrate and 12 is a recording layer provided on the substrate 11. hand,
The recording layer 12 is a layer 12-1 containing a nucleic acid and a layer 1 containing a dye that emits fluorescence upon irradiation with light in the presence of the nucleic acid.
It has a laminated film of 2-2. In such a structure, the nucleic acid contained in the layer 12-1 and the layer 12-
The dyes contained in 2 can be the same as those described above.

Also, in the method of manufacturing this optical recording medium, various methods such as a coating method and a vapor deposition method can be applied similarly to the method of manufacturing the optical recording medium having the structure shown in FIG. When both 12-1 and 12-2 are formed by a coating method, for example, nucleic acid is first coated on a substrate with an aqueous solvent, the nucleic acid layer is dried, and then a fluorescent dye is added. It may be applied with a solvent such as a halogen type which is immiscible with water.

As a method of recording information on the optical recording medium having such a constitution, for example, (i) a state in which a nucleic acid and a dye coexist at the interface between the layer 12-1 and the layer 12-2 is previously formed ( (Initialization), and then irradiating the recording layer 12 with recording light to form pits and record information.
i) at least the layer 12 by irradiating the recording layer 12 with recording light
A method of recording information by forming a state in which a nucleic acid and a dye coexist at the interface between -1 and layer 12-2 can be mentioned.

When recording by the above method (i),
It is preferable that the pits have substantially no fluorescence emission ability, and the optical recording medium on which such recording is performed emits light such that the dye emits in the presence of nucleic acid as reproduction light. By using it, it is possible to reproduce information at a high S / N ratio by detecting the contrast between the fluorescent light emitting portion (information non-recording portion) and the non-light emitting portion (information recording portion).

Further, in the case of recording by the method (ii), it is preferable that the nucleic acid and the dye are sufficiently mixed in the pit portion so that fluorescence emission is caused by irradiation of predetermined light. By using the above-mentioned predetermined light as the reproduction light, the optical recording medium on which such recording is performed has a fluorescent light emitting portion (information recording portion) and a non-light emitting portion (information non-recording portion).
It is possible to reproduce the information having a high S / N ratio by detecting the contrast between and.

[0065]

EXAMPLES Next, the present invention will be described in more detail with reference to examples.

(Synthesis Example 1) Compound 1 in Table 1 was synthesized by the following method.

100 ml of acetic anhydride and 30 ml of concentrated sulfuric acid were mixed while cooling, and the resulting mixed liquid was heated for 3 hours while maintaining the temperature at 80 ° C. in a water bath. 20 ml of acetic anhydride and 30 ml of p-dimethylaminoacetophenone
Is added at room temperature, and then the temperature is raised to 45 ° C.
The reaction was allowed to stir for a period of time. To this reaction solution, an equal amount of ethanol was added, cooled, and an aqueous potassium iodide solution was further added to precipitate crude crystals. The crude crystals were collected by filtration and recrystallized from a mixed system of ethanol / ether to give 2,4-
Green crystals of bis (p-dimethylaminophenyl) -6-methylpyrylium iodide were obtained. Analysis result of the obtained compound 1 Melting point: 254-257 ° C. UV / visible (CH ₃ CN ε × 10 ⁻⁴ ) λmax: 44
4 nm (2.43), 550 nm (8.24) NMR ( ¹ H, DMSO) δ ppm: 8.3737 (1
H, s), 8.2729 (1H, d, J = 9.0H
z), 8.1795 (1H, d, J = 9.0 Hz),
7.8864 (1H, s), 6.9117 (4H, t,
J _AB = J _BC = 9.77), 3.1829 (6H, s),
3.1340 (6H, s), 2.6809 (3H, s) FAB mass m / z 333 IR (KBr) νcm ^-1 : 1645, 1610 (s
h), 1580 (s), 1490 (s), 1270, 1
200, 1160 (Reference Example 1) Compound 1 obtained in Synthesis Example 1 was dissolved in 10 mM phosphate buffer containing 10% acetonitrile to give Compound 1
Was adjusted to a final concentration of 1 × 10 ⁻⁴ M.

On the other hand, a spiral track groove was formed by a 2P method on a glass disk substrate having a diameter of 130 mmφ and a thickness of 1.2 mm, and the solution of the above compound 1 was spin-coated on the track groove forming surface of this disk substrate. Was applied to form a recording layer having a thickness (1000) Å, and a disc of Reference Example 1 was prepared.

Example 1 Compound 1 obtained in Synthesis Example 1
It was dissolved in 10 mM phosphate buffer containing 0% acetonitrile and adjusted so that the final concentration of Compound 1 was 1 × 10 ⁻⁴ M.

Next, Salmon sparm DNA
(Manufactured by Sigma) as TE buffer (10 mM Tris
-1 mM EDTA) and purified by phenol extraction. In order to facilitate handling, the purified product was further digested with the restriction enzyme EcoRI, and the resulting treated solution was treated with D
NA solution was used. A part of this DNA solution and a part of sample I are mixed to give a final DNA concentration of 50 μg / ml.
So that the final concentration of Compound 1 is 1 ×.
The coating solution was adjusted to 10 ^-4 M to prepare a coating solution for the recording layer, and this solution was applied onto the disk substrate prepared in the same manner as in Reference Example 1 by spin coating to form a recording layer having a thickness of 1000 Å. The disk of Example 1 was prepared.

When the absorption spectra of the recording layers of the disc of Reference Example 1 and the disc of Example 1 were measured, it was 570 nm for the disc of Reference Example 1 and 610 nm for the disc of Example 1.

When the fluorescence spectra of the recording layers of the disc of Reference Example 1 and the disc of Example 1 were measured, it was 550n.
When excited with m light, the disk of Reference Example 1 has a wavelength of 650n.
A weak peak was detected in the vicinity of m, whereas the disc of Example 1 had a peak of fluorescence intensity about 100 times that of the disc of Reference Example 1 detected in the vicinity of 650 nm.

From the above results, it can be seen that Compound 1 is a strong intercalator for nucleic acids.

Information was recorded and reproduced on the disk of Example 1 under the following conditions.

That is, Kr ⁺ ion laser (oscillation wavelength 56
Polarization beam splitter (P.
B. The recording light and the reproduction light are separated into two beams by S), and the recording light can be further modulated by an AO modulator.
On a disc rotated at 1800 rpm with 0 mW and a reproducing power of 1 mW, a spot size of 1.
Information was written at 5 μmφ and a recording frequency of 1 MHz and reproduced by reproducing light.

As a result, a reproduced signal with a good S / N ratio was obtained.

Reference Example 2 A disc of Reference Example 2 was prepared in the same manner as in Reference Example 1 except that Compound 16 was used instead of Compound 1 in Reference Example 1.

Example 2 A disk of Example 2 was prepared in the same manner as in Example 1 except that Compound 16 was used instead of Compound 1.

The maximum values of the absorption spectra of the disks of Reference Example 2 and Example 2 are shown in Table 2.

[0080]

[Table 20] Further, when the fluorescence spectra of the recording layers of the disc of Reference Example 2 and the disc of Example 2 were excited by (xenon lamp), a weak peak was detected at 825 nm in the disc of Reference Example 2, whereas that of Example 2 In the disk of No. 3, a peak of fluorescence intensity about 5 times that of the disk of Reference Example 1 was detected near 825 nm.

From the above results, it is found that compound 16 is a nucleic acid intercalator.

Information was recorded and reproduced on the disc of Example 2 under the following conditions.

That is, AlGaAs with an oscillation wavelength of 750 nm
Recording power 10 mW, reproduction power 1 using semiconductor laser
When recording / reproducing was performed in the same manner as in Example 1 at mW, a reproduced signal having a sufficient S / N ratio was obtained.

Example 3 In Example 1, the structure of the recording layer was as shown in FIG.
A disc was prepared in the same manner as in Example 1 except that the layer 12-2 was laminated.

Information was recorded on this disc under the following conditions. When the recording layer of this disc was excited with light of 550 nm, a peak of fluorescence intensity was observed at around 650 nm, and particularly in the information recording area, about 100 times the fluorescence intensity was detected as compared with the non-recording area.

Next, this disc was reproduced under the following conditions.

That is, recording / reproducing was performed in the same manner as in Example 1 except that the laser optical system of Example 1 was used and the recording power was 2 mW and the reproducing power was 0.2 mW.

As a result, a reproduced signal with a sufficient S / N ratio could be obtained.

[0089]

As described above, according to the present invention, it is possible to record and / or reproduce information that can obtain a high quality reproduction signal.

[Brief description of drawings]

FIG. 1 is a schematic sectional view showing an embodiment of an optical recording medium according to the present invention.

FIG. 2 is a schematic sectional view showing another embodiment of the optical recording medium according to the present invention.

FIG. 3 is a schematic sectional view showing another embodiment of the optical recording medium according to the present invention.

FIG. 4 is a schematic cross-sectional view showing another embodiment of the optical recording medium according to the present invention.

FIG. 5 is a schematic cross-sectional view showing another embodiment of the optical recording medium according to the present invention.

[Explanation of symbols]

 1, 11 Substrate 2, 12 Recording layer 3 Protective substrate 4 Adhesive layer 5 Undercoat layer

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display location C09K 11/07 9280-4H G11B 7/00 K 9464-5D 7/24 516 7215-5D // C07D 309/34 (72) Inventor Tsuyoshi Mito 3-30-2 Shimomaruko, Ota-ku, Tokyo Canon Inc.

Claims (19)

[Claims] 1. An optical recording medium comprising a substrate and a recording layer containing a nucleic acid and a dye that emits fluorescence when irradiated with light in the coexistence of the nucleic acid. 2. The optical recording medium according to claim 1, wherein the dye intercalates with the nucleic acid. 3. The optical recording medium according to claim 1, wherein the nucleic acid is DNA, RNA, a double-stranded nucleic acid, or an intramolecular double-stranded portion of a single-stranded nucleic acid. 4. The optical recording medium according to claim 1, wherein the dye is a pyrylium dye represented by the following general formula [I]. [Outer 1] (In the above general formula [I], Represents a heterocycle, X is O, S, Se or Te, represents a pyrylium ring or a pyrylium-like ring, and R ¹ and R ² are each independently a hydrogen atom, a halogen atom, a sulphonate group, an amino group, R represents a styryl group, a nitro group, a hydroxyl group, a carboxyl group, a cyano group, a substituted or unsubstituted lower alkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted lower aralkyl group or a substituted or unsubstituted cycloalkyl group, R ³ is -A or -L-a, L ^{is, -L 1 -, - L 2} -L 3 - or -L ⁴ -L ⁵ -L ⁶ -
And L ^{1 to} L ⁶ are each independently-(CH = C
H) - represents (R ⁴ represents a ring structure having an oxo group) -, a divalent group derived from a substituted or unsubstituted aryl group, a substituted or unsubstituted lower alkylene group, or -CH = R ⁴ , A is a substituted or unsubstituted aryl group, -CH = R ⁵
(R ⁵ represents a substituted or unsubstituted heterocycle, a substituted or unsubstituted cycloalkyl group or a substituted or unsubstituted aromatic ring), and R ¹ or R ^{2 of the} pyrylium ring containing X or a ring similar thereto ,
The hydrogen atom bonded to the carbon atom to which R ³ is not bonded is a halogen atom, a sulfonate group, an amino group, a styryl group, a nitro group, a hydroxyl group, a carboxyl group, a cyano group, a substituted or unsubstituted lower alkyl group, It may be substituted with a substituted or unsubstituted aryl group or a substituted or unsubstituted lower aralkyl group, and Y ⁻ represents an anion. ) 5. The optical recording medium according to claim 4, wherein R ¹ and R ² in the general formula [I] are both substituted or unsubstituted aryl groups. 6. In the general formula [I], the 2-position and 4-position of the heterocycle containing X are substituted with a substituted or unsubstituted aryl group, and any of 3-position, 5-position and 6-position is R ³ The optical recording medium of claim 4, which is replaced with. 7. In the general formula [I], the 3-position and 5-position of the heterocycle containing X are substituted with a substituted or unsubstituted aryl group, and any of 2-position, 4-position and 6-position is R ³ The optical recording medium of claim 4, which is replaced with. 8. In the general formula [I], the 2-position and 6-position of the heterocycle containing X are substituted with a substituted or unsubstituted aryl group, and any of the 3-position, 4-position and 5-position is R ³ The optical recording medium of claim 4, which is replaced with. 9. In the general formula [I], among the carbon atoms constituting the heterocycle containing X, the (n) position and (n +)
The optical recording medium according to claim 4, wherein the carbon atom at position 2) is substituted with a substituted or unsubstituted aryl group (wherein n is a positive integer). 10. In the general formula [I], -L- is a group represented by the following general formula [II], [III], [IV], [V] or [VI]. 9. Optical recording medium of 9. [Outside 3] (In the above general formula [II], Z represents a hydrogen atom or a substituted or unsubstituted lower alkyl group, and n is 0, 1 or 2
Is. ) -Φ- (CH = CH) _n- [III] (In the general formula [III], n is 0, 1 or 2, and Φ is a substituted or unsubstituted o-, m- or p-phenylene group. -CH = CH-Φ-CH = CH- [IV] (wherein Φ is a substituted or unsubstituted o.
Represents a-, m- or p-phenylene group. ) [Outer 4] 11. The optical recording medium according to claim 4, wherein the dye represented by the general formula [I] has the following structure. [Outside 5] (In the above general formula, X represents O or S, and Y ⁻ represents an anion.) 12. The optical recording medium according to claim 4, wherein the dye represented by the general formula [I] has the following structure. [Outside 6] (In the above general formula, X represents O or S, and Y ⁻ represents an anion.) 13. The optical recording medium according to claim 1, wherein the recording layer has a film in which the nucleic acid and the dye are mixed. 14. The optical recording medium according to claim 1, wherein the recording layer has a laminated film of a layer containing the nucleic acid and a layer containing the dye. 15. An optical recording medium having a recording layer containing a nucleic acid and a dye capable of emitting fluorescence by irradiation with light in the coexistence of the nucleic acid on a substrate, and selectively irradiating the recording light to record information. An optical recording method using an optical recording medium, characterized in that 16. The optical recording method according to claim 15, wherein information is recorded by erasing the light emitting ability of the recording layer. 17. The optical recording method according to claim 15, wherein the recording layer is made to exhibit a light emitting ability to record information. 18. A recording layer comprising a nucleic acid and a dye capable of emitting fluorescence by irradiation with a first light in the coexistence of the nucleic acid on a substrate, and the recording is carried out by selective irradiation with a second light. The information is reproduced by irradiating the first light onto the optical recording medium on which information is recorded by selectively erasing the luminous ability of the layer and detecting the contrast between the light emitting portion and the non-light emitting portion. An information reproducing method characterized by performing. 19. A recording layer containing a nucleic acid and a dye capable of emitting fluorescence by irradiation with the first light in the coexistence with the nucleic acid on the substrate, and the recording is carried out by selective irradiation with the second light. Reproducing information by irradiating the first light onto an optical recording medium on which information is recorded by selectively emitting fluorescence from a layer and detecting the contrast between a light emitting portion and a non-light emitting portion. An information reproducing method characterized by: