JPH08266271A - Utilization of useful microorganism - Google Patents

Abstract

PURPOSE: To suppress malodor generated by bacteria in the intestines, smoothen the evacuation to eliminate the undesirable effect of the malodor gas and feces on the body and keep the body in health. CONSTITUTION: A microbial strain having deodorizing power and belonging to Bacillus subtilis which is definitely safe to eat is cultured by pure culture and the obtained living bacterial cells or an agent containing the cells are served for eating.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention suppresses the malodor generated by bacteria in the intestine by eating safe live cells of a microorganism belonging to Bacillus subtilis and its live bacterial agent,
In addition, the present invention relates to a method for smoothing defecation to maintain a healthy body.

[0002]

2. Description of the Related Art Extracts from plants such as yucca and tea leaves have been conventionally used as a method for controlling the foul odor of feces, but they are not as effective as expected. Also,
Although there are various constipation medicines on the market that encourage defecation, they often cause discomfort when used. As described above, a substance for suppressing the bad odor of feces or a drug for smoothing defecation is known, but none is sufficiently satisfactory, and there is no method for suppressing the bad odor of feces and smoothing defecation at the same time.

[0003]

Although the suppression of the foul odor of feces by the extraction from plants used in some health foods and the like is recognized, the effect of smoothing defecation is not seen. Also, many companies sell drugs that smooth defecation, but at the same time, there is no drug that suppresses the odor of feces.
No such drug has ever been developed. Therefore, it is an object of the present invention to provide an invention using safe microorganisms that can simultaneously satisfy these required technical fields.

[0004]

As a conventional method for deodorizing a bad odor, there is a soil deodorizing method in which air containing a bad odor is passed through soil in which microorganisms are present to deodorize.

[0005] Therefore, when Bacillus subtilis, which is a genus of edible safe microorganisms existing in soil, was examined by eating live bacterial cells of a microorganism having deodorizing ability and its live bacterial agent, it was confirmed that the odor of feces was suppressed. However, it was found to smooth bowel movements.

[0006]

[Function] Most of the bacteria that cause malodor in the intestine are facultative anaerobes or anaerobes. It is generally known that all soil deodorizing bacteria that decompose and deodorize the malodor generated by these bacteria are aerobic bacteria.

[0007] Several genus species of Bacillus subtilis, which is a spore-forming bacterium that is less affected by stomach acid etc. when eaten by humans, and is a representative aerobic bacterium that is found anywhere in nature and is safe for the human body, has deodorizing ability. I chose.

[0008] Despite being an aerobic bacterium, it was observed that when it was eaten, oxygen supplementation from the villi of the intestine promoted growth in the intestine, which should be in an anaerobic state, and active generation of a foul-smelling gas. . Microorganisms of these genus species do not grow or stay within the tree for a long period of time and require constant supplementation.

[0009]

EXAMPLES Examples will be shown below, but the present invention is not limited thereto.

Glucose 7g, meat extract 5g in 1L of water,
PH by dissolving 10 g of peptone and 4 g of sodium chloride
A medium having a composition adjusted to 7.0 was put in a 500 ml flask, sterilized at 121 ° C. for 30 minutes, cooled, and then inoculated with Bacillus subtilis purely separated from soil,
The cells were shaken at 24 ° C. for 24 hours. The obtained culture broth was centrifuged, the bacterial cell portion was mixed with the precipitate, dried and then used as a viable cell agent having a viable cell count of 1 × 10 ⁸ cells / g.

Urine was diluted 10 times with water to prepare two 50
After putting 300 ml of each in a 0 ml flask and adding and mixing 5 mg of the probiotic agent to one side and observing the change over time of the odor in the state of standing, the results shown in Table-1 were obtained, and the malodor was suppressed in the probiotic agent. It was confirmed that there is an effect.

[0012] The indication of odor intensity is 1: smell that can be perceived at last, 2: weak odor that can tell what odor it is, 3: odor that can be easily perceived, 4: strong odor.

A total of 250 mg of food was prepared by combining 10 mg of the probiotic agent and 240 mg of the enhancer consisting mainly of lactose. There are individual differences in the amount used, and 1 to 10 / person (3 on average)
However, 120 people (24 men, 96 women)
When I aggregated the questionnaires submitted after eating,
The results shown in Table 2 were obtained. From the above, 80% or more of people who ate the probiotic agent prepared evaluated the effect of suppressing the bad smell of stool odor during defecation and at the same time smoothing the stool.

[0014]

[0015]

INDUSTRIAL APPLICABILITY The present invention is a live microorganism of a microorganism having deodorizing ability, including existing species belonging to Bacillus subtilis (eg, Bacillus subtilis BN1001, Bacillus subtilis Kubota) and novel species which are completely safe to eat. Eat the body and its probiotic agents to control the odor of feces,
It is also a method of smoothing defecation. As a result, it is possible to eliminate a bad influence on the living body due to malodorous gas or feces and to maintain a healthy body.

Claims (1)

[Claims] 1. A Bacillus subtilis
subtilis), which eats live bacterial cells of a microorganism having a deodorizing ability and its live bacterial agent to suppress the foul odor of feces and smooth defecation.