JPH0792161A - Detector for occult blood in stool - Google Patents

Abstract

PURPOSE:To provide a detector for occult blood in stool which can easily detect occult blood while avoiding operational miss and sanitary problems associated therewith. CONSTITUTION:The detector for occult blood in stool comprises a container 11 containing a stool sampling stick 12 filled with a mixing solution, and a cover 15 for enclosing the container 11. A detecting body 20 is loaded to the enclosing cover 15. The detecting body 20 incorporates a part 23 for breaking the seal film 17 of the enclosing cover 15, a filter part 25, and a detection plate 27. The detection plate 27 is provided with an antibody fixing part 30 for trapping antibody sensitizing particles 29 passing together with suspension of stool when it is positive.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fecal occult blood detecting apparatus for detecting fecal occult blood by an immunization method utilizing an antigen-antibody reaction.

[0002]

2. Description of the Related Art A fecal occult blood detection device that can be easily operated by anyone, not only a technician but also an ordinary person (examinee) in colorectal cancer examinations such as mass examinations and next-generation OTC (over-the-counter) medicines, has no doubt. Is being considered. In this case, the fecal occult blood detection device should not be associated with discomfort and insufficiency.

The detection of occult blood in fecal excrement is a very valuable means as a screening method for colorectal cancer with poor initial subjective condition, and it is widely used for early detection and treatment in diagnosis of many pathological conditions such as digestive system. It is carried out in various medical institutions.

Detection methods can be roughly classified into two types. These consist of a chemical method for detecting the iron ion of hemoglobin and an immunization method using an antibody (SPIA method etc.). Due to the fact that there is no need to limit diets and drugs, and because of their high specificity and sensitivity, immunoassays are becoming the mainstream of occult blood detection methods. This immunization method uses a metal sol (SPI, such as gold colloid) as a labeling substance for sensitizing an antibody.
Method A), and occult blood detection methods using dye sol, colored latex, alkaline phosphatase, horseradish peroxidase and other enzymes.

The detection of occult blood in fecal excrement has hitherto been mainly carried out by a person involved in a medical institution, and it is carried out through each step of a stool collection step, a suspension step, a filtration step and a detection judgment step. . Each step is carried out using its own device (stool collection stick, suspension device, etc.). However, nowadays, there is a strong tendency to manage one's own health,
Further, since it leads to early detection of colorectal cancer, which is increasing year by year, it is desired to make the diagnostic agent OTC (over-the-counter drug). Furthermore, if colorectal cancer screening is institutionalized as a health care service for the elderly,
It is considered that the demand for in-situ occult blood detection device will increase more and more in the future in mass screening and the like, and the next OTC will be realized.

[0006]

As described above, the detection of fecal occult blood is carried out by a person involved in a medical institution, but the medical institution alone has few opportunities for screening, and it is necessary to visit a medical institution. There is.

[0007] Further, in the case of OTC, the conventional fecal occult blood test is carried out through various steps such as a stool collection step, a suspension step, a filtration step, a detection determination (coloration determination) step, each with its own device. Since it is used, many operations are required. For this reason, the operation is complicated and cumbersome, and problems such as an operation error or an unsanitary feeling may occur. As described above, the conventional inspection device is complicated in operation at the time of use and cannot be easily used at home.

The present invention has been made in view of the above points, and an object thereof is to provide a fecal occult blood detection apparatus which is easy to operate and excellent in hygiene.

[0009]

The invention according to claim 1 is
A container having a stool collection stick inside and a stool suspension filled with a solution for mixing to form a stool suspension, and a sealing lid attached to the one side opening of the container and having a seal film And a hermetically-sealed detection body mounted on the hermetically-sealed lid, wherein the hermetically-sealed detection body has a cutting portion inside which breaks the seal film when the hermetically-sealed detection body is mounted, and the cutting portion. The fecal occult blood detection apparatus is characterized in that it has a determination unit that is continuously provided to determine the presence or absence of occult blood.

According to a third aspect of the present invention, a fecal sampling stick is housed inside, a container for filling a mixing solution to prepare a fecal suspension, and a container attached to this container and communicating with the inside of the container. The detection body is provided with a possible detection body, and the detection body is provided with a case, a detection plate which penetrates the stool suspension and is arranged substantially orthogonal to the flow direction of the stool suspension, and the case. The antibody-sensitized particles arranged upstream of the detection plate, and the antibody-immobilized portion that traps the antibody-sensitized particles that are fixed to the upstream surface of the detection plate and that pass with the fecal suspension when the positive The fecal occult blood detection device is characterized by the following.

[0011]

According to the invention described in claim 1, the stool collection stick after stool collection is inserted into the container from one side opening, the sealing lid is attached to the container and the container is shaken, and the stool and the solution for mixing are mixed. Mix to make a stool suspension. When the detection body is attached to the sealing lid, the seal film is broken by the cutting part, and the fecal suspension flows into the determination part to determine the presence or absence of occult blood.

According to the third aspect of the invention, the stool collection stick is inserted into the container to prepare a stool suspension.
When the detector is attached to the container, the fecal suspension in the container flows into the detector. In the detection body, the stool suspension flows to the detection plate side together with the antibody-sensitized particles, and when positive, the antibody-sensitized particles are trapped in the antibody fixing portion fixed to the upstream surface of the detection plate.

[0013]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS First Embodiment An embodiment of the present invention will be described below with reference to the drawings. 1 to 5 are views showing a first embodiment of the fecal occult blood detection apparatus according to the present invention.

As shown in FIGS. 1 to 3, the fecal occult blood detection apparatus has a fecal sampling stick 12 having a measuring hole 13 housed therein and a transparent synthetic resin container 11 having one side opened.
And a synthetic resin sealing lid 15 mounted on the opening side of the container 11. Of these, the container 11 is pre-filled with a solution to be mixed with feces. Moreover, the sealing lid 1
5 is mounted on the container 11 by engaging an inner screw 15a provided on the inner peripheral surface with an outer screw 11a provided on the outer peripheral surface of the container 11.

The sealing lid 15 has a sealing film 17 at the end opposite to the container 11, and the sealing lid 1
An outer screw 15b is provided on the outer periphery of the No. 5. Further, the end of the sealing lid 15 on the container 11 side is in contact with a flange 19 provided on the container 11 to maintain the hermeticity.

A detector 20 is attached to the closed lid 15. The detector 20 has a case 21 provided with an inner screw 21a, and the inner screw 21a is attached to the outer lid 15 by engaging the outer screw 15b of the outer lid 15. The case 21 is made of a transparent synthetic resin material, and in the inside thereof, a cutting portion 23, a filter portion 25, a detection plate 27 having an antibody fixing portion 30, and an absorption pad 32 are provided in order from the side of the closed lid 11. ing. Of these, the detection plate 27 having the antibody fixing portion 30 and the absorption pad 32 constitute a determination portion.

As shown in FIG. 2, the cutting section 23 is supported by the case 21 via a supporting section 23a. When the detecting body 20 is attached to the sealing lid 15, the sealing film 17 is provided.
Is designed to break. The filter portion 25 removes impurities in the fecal suspension flowing from the container 11 side, and is configured in three stages in FIGS. 1 and 4. However, the filter unit 25 is not limited to three stages,
It may be less than this or more than this. A large number of antibody-sensitized particles 29 are provided between the detection plate 27 and the filter unit 25. Each particle 29 is made of a metal particle such as gold or silver, a non-metal particle such as selenium, or a colored latex or the like, and preferably has a particle size of 1 nm to 100 nm, and may be provided in the filter portion 25 or on the detection plate 27.

The detection plate 27 is arranged along the longitudinal direction of the case 21 between the filter portion 25 and the absorption pad 32, and permeates the stool suspension and senses the antibody passing along with the stool suspension. When the particles are positive, the antibody fixing unit 30 traps them. The detection plate 27
Is made of nitrocellulose, glass fiber filter, chromatography paper, PVDF (trade name), nylon-based or polyester-based nonwoven fabric, and the like. The antibody fixing section 30 is made of a hemoglobin antibody fixed to the detection plate 27. Further, excess stool suspension and antibody-sensitized particles pass through the detection plate 27 and are absorbed by the absorption pad 32.

Next, the operation of this embodiment having such a configuration will be described. First, the sealing lid 15 is removed from the container 11. Next, stool collection is performed using the stool collection stick 12. In this case, the stool collection stick 12 is stuck to the stool from the side of the measuring hole 13. After that, the stool collection stick 12 is pulled out from the stool, and the stool squeegee (not shown) wipes the stool collection stick 12. In this way, a certain amount of feces is collected in the measuring hole 13. The stool collection stick is not limited to 12 and may be any sticky stick.

Next, the stool collection stick 12 is inserted into the container 11 which has been previously filled with the solution for mixing, and the container 11 is sealed by the sealing lid 15. Next, the container 11 sealed by the sealing lid 15 is shaken well, the stool and the solution are mixed, and the stool suspension is thus obtained.

Subsequently, the case 21 of the detector 20 is attached to the closed lid 15. In this case, the outer screw 15 of the sealing body 15
The inner screw 21a of the case 21 is engaged with b, and the sealing body 15
The case 21 of the detection body 20 is pushed in while being rotated to the outside. When the case 21 is completely attached to the sealing lid 15, the cutting portion 23 breaks the seal film 17,
The fecal suspension in the container 11 flows into the detection body 20 side through the seal film 17. The fecal suspension that has flowed into the detector 20 then passes through the filter unit 25.

Next, the fecal suspension that has passed through the filter portion 25 permeates the detection plate 27 together with the antibody-sensitized particles 29 toward the absorption pad 32 side. When the stool is positive, the antibody-sensitized particles 29 in the stool suspension are trapped in the antibody fixing part 30. The antibody-sensitized particles 29 thus trapped in the antibody-immobilized portion 30 exhibit a color in the antibody-immobilized portion 30, and the result can be confirmed from the outside of the case 21.

During this period, since the excess stool suspension and antibody-sensitized particles are absorbed by the absorbent pad 32, the stool suspension and antibody-sensitized particles 29 can smoothly permeate into the detection plate 27. it can.

As described above, according to this embodiment,
Occult blood can be detected easily and easily. Therefore, an operation error can be eliminated and occult blood can be detected without any hygiene problem.

Next, a modified example of this embodiment will be described with reference to FIGS. The modified examples shown in FIGS. 4 and 5 are different only in the configurations of the detection plate and the absorption pad arranged in the detection body, and other than that are substantially the same as the fecal occult blood detection device shown in FIGS. 1 to 3. .

That is, as shown in FIG. 4 and FIG.
In the case 21 of the detection body 20, a filter part 25 is arranged, and a developing spacer 32a for absorbing the fecal suspension is arranged adjacent to the filter part 25. The case 21 is enlarged at the opposite end of the container 11 (FIG. 1), and an annular pad chamber 33 is formed at the enlarged end. An absorption pad 32b is arranged in the pad chamber 33, and a detection plate 27 having the antibody fixing portion 30 is arranged between the developing spacer 32a and the pad 32b orthogonally to the longitudinal direction of the case 21.

Further, the filter portion 25 and the developing spacer 3
A large number of antibody-sensitized particles 29 are provided between them and 2a.

According to this modification, the impurities in the stool suspension can be more reliably removed by the filter portion 25 and the developing spacer 32a, and the stool suspension can be sent to the detection plate 27 side. The antibody-sensitized particles 29 trapped in the antibody-immobilized portion 30 can be easily confirmed from the bottom side (right side in FIG. 4) of the detection body 20. Second Embodiment FIGS. 6 to 8 are views showing a second embodiment of the fecal occult blood apparatus according to the present invention.

As shown in FIGS. 6 and 7, the fecal occult blood detection device has a fecal sampling stick 12 having a measuring hole 13 housed therein, and a synthetic resin container 11 having one side opened.
A detection body 20 mounted on the opening side of the container 11 via a synthetic resin sealing lid 15 is provided. Of these, container 1
A solution to be mixed with feces is pre-filled in 1. Further, the sealing lid 15 has an inner screw 15a provided on the inner peripheral surface.
Is fitted to the container 11 by engaging an external screw 11a provided on the outer peripheral surface of the container 11.

Further, the sealing lid 15 has a sealing film 17 at the end opposite to the container 11, and the sealing lid 1 is further provided.
An outer screw 15b is provided on the outer periphery of the No. 5. Further, the end of the sealing lid 15 on the container 11 side is in contact with a flange 19 provided on the container 11 to maintain the hermeticity.

The detector 20 has an inner screw 21a and an outer screw 21b.
And a lid case 22 having an inner screw 22a that engages with an outer screw 21b. Both the case 21 and the lid case 22 are made of a synthetic resin transparent body. Further, the detection body 20 is attached to the sealing lid 15 by engaging the inner screw 21a of the case 21 with the outer screw 15b of the sealing lid 15. In the case 21 that constitutes one of the detection bodies 20, a cutting section 23 and a filter section 25 are sequentially arranged from the upstream side to the downstream side (to the right in FIG. 6). A developing spacer 32a for absorbing the fecal suspension is arranged on the downstream side.

Of these, the cutting portion 23 is, as shown in FIG.
The seal film 17 is supported by the case 21 via the support portion 23a, and when the detection body 20 is attached to the sealing lid 15, the seal film 17 is broken. In addition, the filter unit 25
Is for removing impurities in the stool suspension flowing from the container 11 side, and is configured in three stages in FIGS. 6 and 8. However, the number of filter units 25 is not limited to three, and the number may be smaller or larger than this. A large number of antibody-sensitized particles 29 are provided between the filter portion 25 and the developing spacer 32a. Each particle 29 is made of a metal particle such as gold or silver, a non-metal particle such as selenium, or a colored latex or the like, and has a particle diameter of 1 nm to 100 nm.
However, it may be provided in the filter unit 25.

Further, in the lid case 22 which constitutes the other side of the detection body 20, the antibody fixing portion 30 made of hemoglobin antibody is fixed from the upstream side to the downstream side (to the right in FIG. 6), and A detection plate 27 arranged orthogonally to the flow direction of the suspension, a water-soluble film (development delay part) 36, an absorption pad 32b for absorbing excess fecal suspension and antibody-sensitized particles 29, and an anti- The additional detection plate 37 to which the (anti-IgG) antibody portion 38 is fixed is sequentially arranged.

Of these, the antibody-fixing portion 30 is fixed to the upstream surface of the detection plate 27, and the fecal suspension is provided on the upstream surface of the detection plate 27 other than the antibody-fixing portion 30. A barrier portion 35 that prevents the passage of By thus fixing the antibody fixing part 30 to the upstream surface of the detection plate 27, the antibody fixing part 30 and the developing spacer 32a face each other. Therefore, since the fecal suspension after passing through the developing spacer 32a immediately reaches the antibody fixing portion 30,
Antibody-sensitized particles 29 and antibody-immobilized part 3 contained in the stool suspension
The reaction (trap) with 0 is reliably performed. Further, since the barrier section 35 is provided on the upstream surface of the detection plate 27,
The stool suspension that has passed through the developing spacer 32a can be entirely guided to the antibody-immobilized portion 30 side.
The reaction between 9 and the antibody-immobilized portion 30 is further facilitated. The reaction time between the antibody-sensitized particles 29 and hemoglobin in the sample can be adjusted by the length of the developing spacer 32a.

Further, the water-soluble film 36 is used as the detection plate 27.
Is adhered to the downstream surface of, and dissolves in about 3 minutes after coming into contact with, for example, a stool suspension. During this time, the passage of the fecal suspension is prevented so that the reaction time between the antibody-sensitized particles 29 in the fecal suspension and the antibody-immobilized portion 30 is sufficiently long.

Further, the anti-fixing device fixed to the additional detecting plate 37
The (anti-IgG) antibody portion 38 traps unreacted antibody-sensitized particles 29 in the fecal suspension, and is anti- (anti-
IgG) antibody-sensitized particles 29 trapped by the antibody portion 38
Indicates coloration, and this result can be confirmed from the outside of the lid case 22. The anti- (anti-IgG) antibody part 38 is
It shows that the fecal suspension has reached the end of the lid case 22, that is, the end of the reaction, but anti- (anti-IgG)
Instead of the antibody part 38, a leak checker that changes color due to moisture may be provided.

The detection body 20 is constructed by combining a case 21 and a lid case 22.
When the cover case 22 and the lid case 22 are combined, the developing spacer 32
And the antibody fixing part 30 are in contact with each other. The detection plate 27 and the additional detection plate 37 are nitrocellulose, glass fiber filter, chromatography paper, PVD.
It is made of F (trade name), nylon-based or polyester-based non-woven fabric, or the like.

Next, the container 11, the sealing lid 15, the case 21,
The material of the lid case 22 will be described.

These container 11, sealing lid 15 and case 21
Both the lid case 22 and the lid case 22 can be made of a biodegradable material. When a biodegradable material is used, the entire fecal occult blood detection device can be discarded.

Examples of such biodegradable materials include aliphatic polyester (trade name: Bionol (manufactured by Showa High Polymer Co., Ltd.)), microorganism-produced polyester (trade name: Biopol (manufactured by ICI Co.)), polyvinyl alcohol / Examples include starch-based products (trade name: Mataby (manufactured by Novamont)). Container 1 from these materials using normal injection molding
1 etc. can be manufactured.

Next, the water-soluble film 36 and the barrier portion 35 fixed to the detection plate 27 will be described in detail.

Water-soluble film 36 and barrier 35
Has a function of prolonging the time for which the fecal suspension is retained at the reaction site so that the reaction between the antibody-sensitized particles 29 and the antibody-immobilized portion 30 can proceed sufficiently. It is not always necessary if the antibody-sensitized particles 29 and the antibody-immobilized portion 30 can be sufficiently reacted even by allowing the stool suspension to naturally develop and move.

The water-soluble film 36 is made of a water-soluble polymer, and the fecal suspension that has spread and moved is absorbed by the polymer when it comes into contact with the water-soluble polymer. The absorption continues until the amount of the fecal suspension that has spread and moved exceeds the absorption capacity of the polymer. During this period, the stool suspension continues to stay in the polymer, and the reaction between the antibody-sensitized particles 29 in the stool suspension and the antibody-immobilized portion 30 sufficiently progresses during the stay.

As the water-soluble polymer, antibody-sensitized particles 29 are used.
Those which do not affect the binding reaction of are preferable, and specific examples thereof include polyacrylic acid, polyvinyl alcohol,
Examples thereof include polyvinylpyrrolidone, polyacrylamide, polydimethylacrylamide, polyethylene glycol, polypropylene glycol, polyvinyl methyl ether, casein, gelatin, hydroxypropyl cellulose and the like.

Further, instead of the water-soluble film 36, the detection plate 27 may be provided with a porous plate formed by reducing the porosity. , The detection plate 27 on the downstream side of the detection plate 27, for example.
The porous plate may be formed by allowing the presence of particles or fillers smaller than the voids. When the porosity is lowered in this way, resistance is given to the development of the fecal suspension and the development speed is reduced. Preferable examples of the particles or fillers for reducing the porosity include polymer particles such as polystyrene and acryl, micro silica, alumina sol and the like.

The barrier portion 35 can be formed by rendering the upstream surface of the detection plate 27 water-insoluble, for example, with a water-insoluble polymer. As the water-insoluble polymer, those that are substantially insoluble in water and do not affect the binding reaction of the antibody-sensitized particles are preferable, and specific examples thereof include polyacrylate, polymethylmethacrylate, polyvinylacrylate, polybutyl. Two or more kinds of vinyl-based or polymers alone or each monomer such as methacrylate, polystyrene, α-methyl polystyrene, chloromethylated polystyrene, polyvinyl acetate, polybutadiene, polyisoprene, polyacrylonitrile, polyvinyl butyral, polyacrylamide, polyvinyl carbazole, etc. Copolymers composed of combinations, polyamides, polyesters, polyurethanes, etc. obtained by ring-closing polymerization or condensation polymerization, hydroxypropylmethyl cellulose phthalate, carboxymethyl ethyl cellulose, ethyl cellulose Cellulose such as vinegar, shellac, and the like.

Furthermore, a polymer obtained by treating the above-mentioned polymer with a single or more functional ionizing radiation functional monomer or oligomer in the molecule, or a combination of two or more species, or the above polymer, is also preferably used. The compound having a functional group in the molecule is preferably a compound which basically reacts to ionizing radiation such as ultraviolet rays and electron beams, and particularly a compound having a radical-polymerizable unsaturated bond. Monofunctional monomers include acrylic acid, acrylic acid dimer, 2-hydroxyethyl acrylate, 2-hydroxypropyl acrylate, methyl acrylate, 2-ethylhexyl acrylate, methoxyethyl acrylate, butyl acrylate, butyl acrylate, methoxybutyl acrylate, phenyl acrylate, etc. Acrylic acid and its esters; Methacrylic acid esters; acrylamide, pro Le acrylamide, methacrylamide, unsaturated carboxylic acid amides of methacrylamide; 2- (N, N-
Dimethylamino) ethyl acrylate, 2- (N, N-
Dimethylamino) ethyl methacrylate, 2- (N, N
-Dibenzylamino) ethyl acrylate, (N, N-
Dimethylamino) methyl methacrylate, 2- (N, N
-Substituted amino alcohol esters of unsaturated acids such as -diethylamino) propyl acrylate; N-methylcarbamoyloxyethyl acrylate, N-ethylcarbamoyloxyethyl acrylate, N-butylcarbamoyloxyethyl acrylate, N-phenylcarbamoyloxy Carbamoyloxyalkyl acrylates such as ethyl acrylate, 2- (N-methylcarbamoyloxy) ethyl acrylate, 2-carbamoyloxypropyl acrylate, etc. Other vinylbenzyltrimethylammonium chloride, N-methyl-4-vinylpyridinium chloride, 2- Cationic vinyl monomers such as methacryloyloxyethyltrimethylammonium chloride and 2-acroyloxydimethylsulfonium chloride, acryloylmorpholine And the like.

The polyfunctional monomer has two or more radically polymerizable unsaturated groups, and a polyfunctional (meth) acrylate having two or more (meth) acryloyl groups is particularly preferable. Specific examples include, for example, ethylene glycol di (meth) acrylate, polyethylene glycol di (meth) acrylate, hexanediol di (meth) acrylate, trimethylolpropane tri (meth) acrylate, trimethylolpropane di (meth).
Acrylate, pentaerythritol tetra (meth) acrylate, pentaerythritol tri (meth) acrylate, dipentaerythritol hexa (meth) acrylate, ethylene glycol diglycidyl ether (meth) acrylate, polyethylene glycol diglycidyl ether di (meth) acrylate, propylene glycol Examples thereof include polyfunctional (meth) acrylates such as diglycidyl ether di (meth) acrylate, polypropylene glycol diglycidyl ether di (meth) acrylate, sorbitol tetraglycidyl ether tetra (meth) acrylate, and melamine (meth) acrylate.

As the oligomer or polymer having a functional group, for example, a urethane acrylate obtained by bonding a diisocyanate, a diol having hydroxyl groups at both ends and an acrylate compound having a hydroxyl group at both ends and a radical polymerizable unsaturated group is bonded. Yes, a urethane acrylate having an arbitrary molecular weight can be obtained by changing the molecular weight of the diol used. In addition, a 1: 1 adduct of acrylic acid chloride or 2-hydroxyethyl acrylate and diisocyanate is added to the hydroxyl group of the copolymer of polyester acrylate, epoxy acrylate, acrylic acid alkyl ester and acrylic acid-2-hydroxy ester. Acryloyl group-introduced copolymer, a copolymer in which glycidyl rotacrylate is added to the carboxyl group of a copolymer of acrylic acid alkyl ester and acrylic acid, or acrylic acid chloride or acrylic acid on the residual hydroxyl group of polyvinyl butyral. Those obtained by adding a 1: 1 adduct of 2-hydroxyethyl acid and diisocyanate are also preferably used.

The above-mentioned water-soluble film 36 and barrier portion 35 are formed by dissolving or dispersing a water-soluble polymer and a water-insoluble polymer in an appropriate solvent and applying them to both sides of the detection plate 27 by pipetting. . According to a preferred embodiment, these may be applied using printing techniques. For example, a water-soluble polymer or a water-insoluble polymer is dissolved in an appropriate solvent (eg, water, alcohols, esters, ethers, aldehydes, ketones, benzenes, paraffins, etc., alone or in a mixed solvent). Both surfaces of the detection plate 27 are impregnated with the above composition by a method such as gravure printing, screen printing, blade coating, slide coating and the like.

Next, the operation of this embodiment having such a configuration will be described. First, the sealing lid 15 is removed from the container 11. Next, stool collection is performed using the stool collection stick 12. In this case, the stool collection stick 12 is stuck to the stool from the side of the measuring hole 13. After that, the stool collection stick 12 is pulled out from the stool, and the stool squeegee (not shown) wipes the stool collection stick 12. In this way, a certain amount of feces is collected in the measuring hole 13. The stool collection stick is not limited to 12 and may be any sticky stick.

Next, the stool collection stick 12 is inserted into the container 11 which is filled with the solution for mixing in advance, and the container 11 is sealed by the sealing lid 15. Next, the container 11 sealed by the sealing lid 15 is shaken well, the stool and the solution are mixed, and the stool suspension is thus obtained.

Subsequently, the detection body 20, which is constructed by combining the case 21 and the lid case 22 in advance, is attached to the closed lid 15. In this case, the outer screw 15b of the sealing body 15 is attached to the case 21.
The inner screw 21a is engaged and the case 21 of the detection body 20 is pushed into the outside of the closed body 15 while rotating. When the case 21 is completely attached to the sealing lid 15, the cutting portion 23 breaks the seal film 17, and the fecal suspension in the container 11 flows into the detector 20 side through the seal film 17. The fecal suspension that has flowed into the detector 20 then passes through the filter unit 25.

The stool suspension that has passed through the filter portion 25 passes through the developing spacer 32a together with the antibody-sensitized particles 29, and reacts with the antibody fixing portion 30 fixed on the upstream side of the detection plate 27. In this case, if positive, the antibody-sensitized particles 29 are trapped in the antibody fixing part 30 via hemoglobin, and the positive result is confirmed by visually checking the antibody fixing part 30 by removing the lid case 22 from the case 21. You can

Next, the fecal suspension and the unreacted antibody-sensitized particles 29 reach the water-soluble film 36 fixed on the downstream side of the detection plate 27 from the detection plate 27. Then, after dissolving the water-soluble film 36, the stool suspension and the antibody-sensitized particles 2
9 is absorbed by the absorption pad 32b and reaches the additional detection plate 37. Here, the antibody-sensitized particles 29 react with the anti- (anti-IgG) antibody portion 38 fixed to the additional detection plate 37, and show a color. By confirming the result of this coloration from the outside, the end time of the reaction is determined. (Specific Example) Next, a specific example of this embodiment will be described. A glass fiber filter (GF / DVA, manufactured by Whatman) is cut into a size equal to the inner diameter of the lid case 22 to detect the body 2.
7, and an anti-hemoglobin antibody solution (manufactured by Denka Seiken Co., Ltd.) was fixed to the center thereof to form the antibody-immobilized portion 30. Next, there is a resistance between the inside of the lid case 22 and the absorbent pad 32b.
An additional detection plate 27b to which an (anti-IgG) antibody (manufactured by Cosmo Bio Co., Ltd.) was fixed was arranged and used as a termination sign. As the antibody-sensitized particles 29, an anti-hemoglobin antibody-sensitized gold colloidal solution (manufactured by Meiji Seika Co., Ltd.) is used and impregnated into a non-woven fabric (Benlize PO-500, manufactured by Asahi Kasei Co., Ltd.), and the filter section 25 and the developing spacer are used. It was arranged between the parts 32a. A 1 μg / ml hemoglobin solution was used as a test substance.
7 ml was developed. When all the samples were developed and the end sign was confirmed, the lid case 22 was removed from the case 21, and a reddish purple coloration of the antibody-immobilized portion 30 was confirmed.

Next, a modification of this embodiment will be described with reference to FIG. The modified example shown in FIG. 8 is different only in the structure of the lid case 22, and is otherwise substantially the same as the fecal occult blood detection apparatus shown in FIGS. 6 and 7.

That is, as shown in FIG. 8, the lid case 2
2 is enlarged at the downstream (right side in FIG. 8) end, and an annular pad chamber 33 in which the absorbent pad 32b is arranged is formed at the enlarged end. Further, in the lid case 22, a detection plate 27 having an antibody fixing portion 30 and a barrier portion 35 provided on the upstream surface is arranged substantially orthogonal to the flow direction of the fecal suspension.

[0058]

As described above, according to the present invention of claim 1, occult blood can be detected easily and easily. Therefore, it is possible to prevent an operation error and avoid a hygiene problem associated with the operation error.

According to the third aspect of the present invention, the antibody-sensitized particles flowing to the detection plate side immediately reach the antibody-immobilized portion fixed to the upstream side of the detection plate. The reaction with can be performed easily and surely.

[Brief description of drawings]

FIG. 1 is a side sectional view showing a first embodiment of a fecal occult blood detection apparatus according to the present invention.

FIG. 2 is a sectional view of the fecal occult blood detection device of FIG. 1 taken along the line II-II.

FIG. 3 is a sectional view taken along line III-III of the fecal occult blood detection device of FIG. 1.

FIG. 4 is a partial side sectional view showing a modified example of the fecal occult blood detection device according to the present invention.

5 is a cross-sectional view taken along line VV of the fecal occult blood detection device of FIG.

FIG. 6 is a side sectional view showing a second embodiment of the fecal occult blood detection apparatus according to the present invention.

7 is a sectional view taken along line VII-VII of the fecal occult blood detection device of FIG.

FIG. 8 is a partial side sectional view showing a modified example of the fecal occult blood detection device according to the present invention.

[Explanation of symbols]

 11 Container 12 Fecal Stick 15 Sealing Lid 17 Sealing Film 20 Detector 21 Case 22 Lid Case 23 Cutting Part 25 Filter Part 27 Detection Plate 29 Antibody Sensitizing Particle 30 Antibody Fixing Part 32a Deployment Spacer 32b Absorption Pad 35 Barrier Part 36 Water-soluble Film 37 Additional detection plate 38 IgG antibody part

Claims (6)

[Claims] 1. A container in which a stool collection stick is housed and which is filled with a solution for mixing to form a stool suspension and which has one side opened, and which is attached to the one side opening and sealed. A hermetically-sealing lid having a film, and a hermetically-sealed detection body mounted on the hermetically-sealing lid, wherein the detection body has a cutting portion that breaks the seal film when the detection body is mounted on the hermetically-sealing lid. And an in-stool occult blood detection device, which is provided continuously with the cutting unit and determines the presence or absence of occult blood. 2. The determination unit includes a detection plate for permeating a stool suspension,
An antibody fixing part attached to this detection plate for trapping antibody-sensitized particles that pass along with the stool suspension at the time of positive, and a stool suspension and antibody-sensitized particles that have passed through and are arranged on the downstream side of the detection plate. The fecal occult blood detection device according to claim 1, which is composed of an absorber for carrying out the treatment. 3. A container for accommodating a stool collection stick and filling a mixing solution to prepare a stool suspension, and a detector attached to the container and capable of communicating with the inside of the container. This detection body is provided with a case, a detection plate which is provided in the case and which penetrates the stool suspension and is arranged substantially orthogonal to the flow direction of the stool suspension, and an upstream side of the detection plate in the case. Stool characterized in that it has an antibody-sensitized particle disposed on the side and an antibody-immobilized portion that traps the antibody-sensitized particle that is fixed to the upstream surface of the detection plate and that passes with the stool suspension when positive. Internal occult blood detector. 4. A detector, which is disposed in the case downstream of the detector plate and absorbs fecal suspension and antibody-sensitized particles, and a development body disposed between the detector plate and the absorber. The fecal occult blood detection device according to claim 3, further comprising a delay unit. 5. The fecal occult blood detection device according to claim 3, wherein the detection body is separable into an upstream side and a downstream side with the surface of the detection plate on the side of the antibody fixing portion as a boundary. 6. The fecal occult blood detection according to claim 3, wherein a barrier portion that prevents passage of the fecal suspension is provided on the upstream surface of the detection plate other than the antibody fixing portion. apparatus.