JPH0769312B2 - Simultaneous analysis method for nitrogen-containing ions and its equipment - Google Patents
Simultaneous analysis method for nitrogen-containing ions and its equipmentInfo
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- JPH0769312B2 JPH0769312B2 JP30379787A JP30379787A JPH0769312B2 JP H0769312 B2 JPH0769312 B2 JP H0769312B2 JP 30379787 A JP30379787 A JP 30379787A JP 30379787 A JP30379787 A JP 30379787A JP H0769312 B2 JPH0769312 B2 JP H0769312B2
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- nitrogen
- ion
- containing ions
- exchange column
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Description
【発明の詳細な説明】 （イ）産業上の利用分野 この発明は含窒素イオンの一斉分析法およびその装置に
関する。さらに詳しくはイオンクロマトグラフィを用い
た高速液体クロマトグラフィにより、亜硝酸イオン、硝
酸イオン、アンモニウムイオン等の含窒素イオンを一斉
に分析する方法およびその装置に関する。TECHNICAL FIELD The present invention relates to a simultaneous analysis method for nitrogen-containing ions and an apparatus therefor. More specifically, it relates to a method and apparatus for simultaneously analyzing nitrogen-containing ions such as nitrite ions, nitrate ions and ammonium ions by high performance liquid chromatography using ion chromatography.
（ロ）従来の技術 亜硝酸イオン、硝酸イオン、アンモニウムイオン等の無
機含窒素イオンの形態別分析を行うことは、主に土壌、
水中のバクテリアによる雰囲気等を分析する上で重要な
意義を有する。ことにイオンクロマトグラフィは、上記
無機含窒素イオンの濃度を、形態別に高感度分析するこ
とが可能なため、これらの分析に対して汎用されてい
る。(B) Conventional technology The analysis of inorganic nitrogen-containing ions such as nitrite ion, nitrate ion, and ammonium ion by morphology is mainly conducted in soil,
It has important significance in analyzing the atmosphere of bacteria in water. In particular, ion chromatography is widely used for these analyzes because it is possible to analyze the concentration of the above-mentioned inorganic nitrogen-containing ions with high sensitivity according to the form.
（ハ）発明が解決しようとする問題点 しかしながら、通常のイオンクロマトグラフィを用いた
場合、上記無機含窒素イオンのうちで亜硝酸イオンと硝
酸イオンの分析を、アンモニウムイオンの分析とは別個
に行う必要がある。このため操作性、信頼性の点から考
えると、単一流路（シングルラン）による全成分の同時
一斉分析を行うことが望ましい。(C) Problems to be Solved by the Invention However, when ordinary ion chromatography is used, it is necessary to analyze nitrite ion and nitrate ion among the above inorganic nitrogen-containing ions separately from the analysis of ammonium ion. There is. Therefore, from the viewpoint of operability and reliability, it is desirable to perform simultaneous simultaneous analysis of all components by a single flow path (single run).
この発明はかかる状況に鑑みなされたものであり、特定
の移動相を選択することにより、含窒素イオン、ことに
無機含窒素イオンの一斉分析が可能な分析法および装置
を提供しようとするものである。The present invention has been made in view of such circumstances, and it is an object of the present invention to provide an analysis method and an apparatus capable of simultaneous analysis of nitrogen-containing ions, particularly inorganic nitrogen-containing ions, by selecting a specific mobile phase. is there.
（ニ）問題点を解決するための手段 かくしてこの発明によれば、含窒素イオンを含有する試
料を、紫外吸収を持たないpHが３以上の酸性水溶液を移
動相とし、かつ、陽イオン交換カラムおよび陰イオン交
換カラムとを上記移動相流路に直列に接続したイオンク
ロマトグラフィに付して上記含窒素イオンを分離し、こ
の分離される陽イオンフラクションを電気伝導度検出器
で、同じく陰イオンフラクションを紫外吸光検出器でそ
れぞれ検出することを特徴とする含窒素イオンの一斉分
析法が提供される。(D) Means for Solving the Problems Thus, according to the present invention, a sample containing nitrogen-containing ions is used as a mobile phase in an acidic aqueous solution having a pH of 3 or more without ultraviolet absorption, and a cation exchange column. And an anion exchange column are connected to the mobile phase flow path in series by ion chromatography to separate the nitrogen-containing ions, and the separated cation fraction is analyzed by an electric conductivity detector to detect the anion fraction. A simultaneous analysis method for nitrogen-containing ions is provided, which comprises detecting each of them with an ultraviolet absorption detector.
この発明は、含窒素イオンことに無機含窒素イオンを形
態別に分別することなく、これらのイオンが含まれた試
料を特定の酸性移動相を用いたシングルラン構成のイオ
ンクロマトグラフィにより、各形態毎の濃度を一斉に測
定できうる方法であることを特徴とする。This invention does not separate the nitrogen-containing ions into inorganic nitrogen-containing ions by form, and samples containing these ions are subjected to ion chromatography in a single run configuration using a specific acidic mobile phase. The method is characterized in that the concentration can be measured all at once.
この発明の方法は、土壌、水中のバクテリア雰囲気を知
るうえで重要な、亜硝酸イオン、硝酸イオン、アンモニ
ウムイオン等からなる無機含窒素イオンを一斉分析する
のに好適な方法である。The method of the present invention is a method suitable for simultaneous analysis of inorganic nitrogen-containing ions such as nitrite ion, nitrate ion and ammonium ion, which are important for knowing the bacterial atmosphere in soil and water.
この発明の方法において、上記含窒素イオンのうち陽イ
オンは陽イオン交換カラムにより、陰イオンは陰イオン
交換カラムによりそれぞれ分離される。従ってこれらの
イオンをシングルランで分離するには、上記陽イオン交
換カラムおよび陰イオン交換カラムを直列に管路接続し
たイオン交換カラム部を用いて行われる。この場合これ
らのカラムの接続順序はいずれが先であってもよい。上
記用いられる陽イオン交換カラムおよび陰イオン交換カ
ラムは、それぞれ交換容量が通常のイオンクロマトグラ
フィ用のもの以下のものが選択される。この場合、陽イ
オン交換カラムでは25μeq/g以下が、陰イオン交換カラ
ムでは15μeq/g以下が適している。In the method of the present invention, among the nitrogen-containing ions, cations are separated by a cation exchange column and anions are separated by an anion exchange column. Therefore, the separation of these ions in a single run is performed using the ion exchange column section in which the cation exchange column and the anion exchange column are connected in series by a pipe line. In this case, any of these columns may be connected first. As the cation exchange column and the anion exchange column used above, those having exchange capacities below those for ordinary ion chromatography are selected. In this case, 25 μeq / g or less is suitable for the cation exchange column, and 15 μeq / g or less is suitable for the anion exchange column.
この発明の方法に用いる移動相は、上記のごとく直列に
管路接続されたイオン交換カラム部を移送される間に、
分析対象の陽イオンと競合しうる陽イオンと、分析対象
の陰イオンと競合しうる陰イオンとを供給でき、かつ、
陽イオン交換カラム部で分離保持された分析対象の陽イ
オンを溶出するとともに、陰イオン交換カラム部で分離
保持された分析対象の陰イオンを溶出しうるものが選択
される。上記競合する陽イオンとしては水素イオンが用
いられ従って酸性移動相が適しているが、ただし分析対
象の陰イオンである亜硝酸イオンは弱酸イオンであるた
め、上記用いる酸性移動相の酸性度が強い場合この陰イ
オンの解離が抑制されることが生ずる。このため上記酸
性移動相は、上記亜硝酸の産解離定数（pKa＝3.2）か
ら、そのpHが３以上、好ましくは3.5以上に調製されて
用いられる。また、この発明の方法において上記分析対
象の陰イオン（亜硝酸イオン、硝酸イオン等）は、後述
するごとくこれらのイオンが有する紫外吸収性に基づい
て検出するため、従って上記酸性移動相はさらに紫外吸
収性を有しないことが必要となる。このことから上記移
動相を構成する陰イオンとしては過塩素酸イオン、硫酸
イオン、ニチオン酸イオン等が選択される。The mobile phase used in the method of the present invention, while being transferred through the ion exchange column section connected in series as described above,
A cation that can compete with the cation to be analyzed and an anion that can compete with the anion to be analyzed can be supplied, and
Those capable of eluting the cations of the analysis target separated and retained by the cation exchange column section and the eluting of the anions of the analysis target separated and retained by the anion exchange column section are selected. Hydrogen ions are used as the competing cations, and therefore an acidic mobile phase is suitable, but since the anion to be analyzed, nitrite ion, is a weak acid ion, the acidity of the acidic mobile phase used is strong. In this case, the dissociation of the anion may be suppressed. Therefore, the pH of the acidic mobile phase is adjusted to 3 or higher, preferably 3.5 or higher, according to the dissociation constant (pKa = 3.2) of nitrite. Further, in the method of the present invention, the anion to be analyzed (nitrite ion, nitrate ion, etc.) is detected based on the ultraviolet absorptivity of these ions as will be described later. It is necessary to have no absorbency. From this, perchlorate ion, sulfate ion, dithionate ion and the like are selected as the anions constituting the mobile phase.
この発明の方法において、上記イオン交換カラム部で分
離・溶出される無機含窒素イオンは、電導度または紫外
吸光度に基づいてそれぞれ検出される。すなわち、アン
モニウムイオンは電導度で、亜硝酸イオンおよび硝酸イ
オンは紫外吸光度で検出される。従って検出に必要な電
気伝導度検出器および紫外吸光検出器は前記移動相流路
に直列に管路接続して用いられる。この場合接続順序は
いずれが上流側に設定されても良いが、しかしながら電
気伝導度検出器は恒温雰囲気下で用いることが必要なた
め、前記イオン交換カラム部に通常設定される恒温槽を
利用できる点で、電気伝導度検出器が上流側に設定され
ることが好ましい。In the method of the present invention, the inorganic nitrogen-containing ions separated / eluted in the ion exchange column section are respectively detected based on the conductivity or the ultraviolet absorbance. That is, ammonium ion is detected by conductivity, and nitrite ion and nitrate ion are detected by ultraviolet absorption. Therefore, the electric conductivity detector and the ultraviolet absorption detector necessary for the detection are used by connecting the mobile phase flow path in series with the pipeline. In this case, any connection order may be set on the upstream side. However, since the electric conductivity detector needs to be used in a constant temperature atmosphere, a constant temperature bath normally set in the ion exchange column section can be used. At this point, the conductivity detector is preferably set on the upstream side.
以上の分析方法を実施するにあたって、この発明は次に
挙げるごとき基本的構成を有する含窒素イオン一斉分析
装置を提供することができる。すなわち、紫外吸収を持
たないpHが３以上の酸性水溶液からなる移動相供給部、
試料導入口、陽イオン交換カラムおよび陰イオン交換カ
ラムとが直列に接続されたイオンクロマトカラム部、電
気伝導度検出器および紫外吸光検出器が直列に接続され
た含窒素イオン検出部をこの順に管路接続した分析流路
で構成されてなる含窒素イオン一斉分析装置が提供され
る。In carrying out the above-described analysis method, the present invention can provide a nitrogen-containing ion simultaneous analysis device having the following basic constitution. That is, a mobile phase supply unit composed of an acidic aqueous solution having a pH of 3 or more without ultraviolet absorption,
A sample inlet, an ion chromatographic column part in which a cation exchange column and an anion exchange column are connected in series, and a nitrogen-containing ion detection part in which an electrical conductivity detector and an ultraviolet absorption detector are connected in series are connected in this order. Provided is a nitrogen-containing ion simultaneous analysis device constituted by analytically connected analysis channels.
（ホ）作用 この発明によれば、紫外吸収性を持たないpHが３以上の
酸性移動相により移送される無機含窒素イオンは、該移
動相流路に直列に接続された陽イオン交換カラムおよび
陰イオン交換カラムからなるイオン交換カラム部におい
て、その含窒素イオンのうち陽イオン類が陽イオン交換
カラムで、その含窒素イオンのうち陰イオン類が陰イオ
ン交換カラムでそれぞれ分離され次いで溶出される。こ
れらの溶出された各イオンフラクションはさらに移送さ
れ、上記移動相流路に直列に接続された電気伝導度検出
器および紫外吸光検出器からなる検出部を通過する際、
上記陽イオンフラクションはその電導度に基づいて検出
され、上記陰イオンフラクションはその紫外吸光度に基
づいてそれぞれ検出される。(E) Action According to the present invention, the inorganic nitrogen-containing ions transferred by the acidic mobile phase having no ultraviolet absorption property and having a pH of 3 or more are used in the cation exchange column and the cation exchange column connected in series to the mobile phase channel. In the ion-exchange column section consisting of an anion-exchange column, the cations of the nitrogen-containing ions are separated in the cation-exchange column and the anions of the nitrogen-containing ions are separated in the anion-exchange column and then eluted. . Each of these eluted ion fractions is further transferred, and when passing through a detection unit composed of an electric conductivity detector and an ultraviolet absorption detector connected in series to the mobile phase flow path,
The positive ion fraction is detected based on its conductivity, and the negative ion fraction is detected based on its ultraviolet absorbance.
以下実施例によりこの発明を詳細に説明するが、これに
よりこの発明は限定されるものではない。Hereinafter, the present invention will be described in detail with reference to Examples, but the present invention is not limited thereto.
（ヘ）実施例 第１図はこの発明の方法を実施する含窒素イオン一斉分
析装置（１）の一例の構成説明図である。図において該
装置（１）は、移動相貯留槽（２）、送液ポンプ
（３）、試料導入口（４）、予熱部（５）、陽イオン交
換カラム（６）、陰イオン交換カラム（７）、電気伝導
度検出器（８）、紫外分光光度計検出器（９）およびド
レイン（10）をこの順に管路接続した分析流路（ａ）で
主として構成されている。（11）は恒温槽、（12）はデ
ータ処理装置である。上記のごとき構成を有する装置と
しては例えば、Shimadzu HIC−6A,dual detection syst
em〔（株）島津製作所製〕として入手可能である。(F) Example FIG. 1 is a structural explanatory view of an example of a nitrogen-containing ion simultaneous analyzer (1) for carrying out the method of the present invention. In the figure, the device (1) includes a mobile phase storage tank (2), a liquid feed pump (3), a sample inlet (4), a preheating section (5), a cation exchange column (6), and an anion exchange column ( 7), an electric conductivity detector (8), an ultraviolet spectrophotometer detector (9), and a drain (10) are mainly connected to each other in this order, and are composed of an analysis channel (a). (11) is a thermostat, and (12) is a data processor. As an apparatus having the above configuration, for example, Shimadzu HIC-6A, dual detection syst
It is available as em [manufactured by Shimadzu Corporation].
以上のごとく構成された装置（１）は、検出限界が下記
の通りである。検出限界（S/N＝２） NO2−Ｎ 150pg NO3−Ｎ 110pg NH3−Ｎ 50pg 上記構成の装置（１）において下記分析条件に設定し
て、亜硝酸イオン、硝酸イオン、アンモニウムイオンを
所定濃度で含有する標準試料（イ）および（ロ）につい
て（前者を５μ，後者を10μ）分析し、それぞれ第
２図および第３図に示すクロマトグラムを得た。The detection limit of the device (1) configured as described above is as follows. Detection limit (S / N = 2) NO 2 -N 150 pg NO 3 -N 110 pg NH 3 -N 50 pg Set the following analysis conditions in the device (1) with the above configuration, and set nitrite ion, nitrate ion, ammonium ion The standard samples (a) and (b) contained at a predetermined concentration (5 μ for the former and 10 μ for the latter) were analyzed to obtain the chromatograms shown in FIGS. 2 and 3, respectively.
移動相:0.1mM HClO4（pH＝4.05） カラム：陽イオン交換カラム 陰イオン交換カラム カラム温度:40℃ 移動相流量:1.5ml/min 検出器:Shimadzu CDD−6AおよびSPD−6A（λ＝210nm） 〔標準試料〕 （イ）:NO2−Ｎ 2.5ppm NO3−Ｎ ５ ppm NH3−Ｎ ５ ppm （ロ）:NO2−Ｎ 50 ppb NO3−Ｎ 100 ppb NH3−Ｎ 200 ppb 第２図において、電気伝導度検出器（CDD−6A）による
クロマトグラム（ｉ）では（←）位置にNH4 +のピークが
検出されており、一方紫外分光光度計検出器（SPD−6
A）によるクロマトグラム（ii）では（←）位置にNO2 -
およびNO3 -のピークがそれぞれ検出されている。Mobile phase: 0.1 mM HClO 4 (pH = 4.05) Column: Cation exchange column Anion exchange column Column temperature: 40 ° C Mobile phase flow rate: 1.5 ml / min Detector: Shimadzu CDD-6A and SPD-6A (λ = 210 nm) ) [Standard sample] (a): NO 2 -N 2.5 ppm NO 3 -N 5 ppm NH 3 -N 5 ppm (b): NO 2 -N 50 ppb NO 3 -N 100 ppb NH 3 -N 200 ppb No. In Fig. 2, the peak of NH 4 + is detected at the (←) position in the chromatogram (i) by the conductivity detector (CDD-6A), while the UV spectrophotometer detector (SPD-6) is detected.
Chromatogram (ii) by A) (←) NO in position 2 -
And NO 3 − peaks are detected respectively.
一方第３図において、電気伝導度検出器（CDD−6A）に
よるクロマトグラム（iii）では（←）位置にNH4 +のピ
ークが検出されており、紫外分光光度計検出器（SPD−6
A）によるクロマトグラム（iv）では（←）位置にNO2 -
およびNO3 -のピークがそれぞれ検出されている。On the other hand, in FIG. 3, the NH 4 + peak is detected at the (←) position in the chromatogram (iii) by the electric conductivity detector (CDD-6A), and the ultraviolet spectrophotometer detector (SPD-6
Chromatogram (iv) by A) (←) NO in position 2 -
And NO 3 − peaks are detected respectively.
またさらに上記装置（１）によって下水を分析したとこ
ろ、第４図に示す結果を得た。該図において、電気伝導
度検出器（CDD−6A）によるクロマトグラム（ｖ）では
（←）位置にNH4 +のピークが検出されており、紫外分光
光度計検出器（SPD−6A）によるクロマトグラム（vi）
では（←）位置にNO2 -およびNO3 -のピークがそれぞれ検
出されていることが確認された。Further, when the sewage was analyzed by the device (1), the results shown in FIG. 4 were obtained. In the figure, the NH 4 + peak is detected at the (←) position in the chromatogram (v) by the electric conductivity detector (CDD-6A), and the chromatogram by the ultraviolet spectrophotometer detector (SPD-6A) is detected. Grams (vi)
In (←) NO 2 in position - and NO 3 - peak of it was confirmed to have been detected, respectively.
以上の結果から、この発明の装置（１）によれば、N
H4 +,NO2 -,NO3 -を一斉に検出することができる。さらに
この装置によれば高感度で検出することができる。From the above results, according to the device (1) of the present invention, N
H 4 +, NO 2 -, NO 3 - can be detected all at once. Further, according to this device, detection can be performed with high sensitivity.
（ト）発明の効果 この発明によれば、一回の分析で含窒素イオンことに無
機含窒素イオンの検出が一斉に行えるため、操作性に優
れかつ分析精度が向上する。移動相に非常に希薄な酸水
溶液を用いるため、電気伝導度検出器のバックグラウン
ドが低減し、ノイズレベルも低くなるうえ、亜硝酸イオ
ン、硝酸イオンを紫外吸光検出器で測定するよう構成し
ているので、通常のイオンクロマトグラフィで別々に測
定するより高感度で検出することができる。(G) Effect of the Invention According to the present invention, since the nitrogen-containing ions and the inorganic nitrogen-containing ions can be simultaneously detected in one analysis, the operability is excellent and the analysis accuracy is improved. Since a very dilute aqueous acid solution is used for the mobile phase, the background of the conductivity detector is reduced, the noise level is also low, and it is configured to measure nitrite ion and nitrate ion with an ultraviolet absorption detector. Therefore, it is possible to detect with higher sensitivity than that which is separately measured by ordinary ion chromatography.
第１図はこの発明の方法を実施する含窒素イオン一斉分
析装置の一例の構成説明図、第２図は、第１図の装置に
よる無機含窒素イオンを含有する標準試料についてのク
ロマトグラム図、第３図は第１図の装置による無機含窒
素イオンを含有する標準試料についての高感度分析をし
たときのクロマトグラム図、第４図は第１図の装置によ
り下水を分析したときのクロマトグラム図である。 （２）……移動相貯留槽、（３）……送液ポンプ、 （４）……試料導入口、（５）……予熱部、 （６）……陽イオン交換カラム、 （７）……陰イオン交換カラム、 （８）……電気伝導度検出器、 （９）……紫外分光光度計検出器 （11）……恒温槽、（12）……データ処理装置、 （ａ）……分析流路。FIG. 1 is an explanatory diagram of an example of a nitrogen-containing ion simultaneous analysis device for carrying out the method of the present invention, and FIG. 2 is a chromatogram diagram of a standard sample containing inorganic nitrogen-containing ions by the device of FIG. FIG. 3 is a chromatogram diagram when high-sensitivity analysis was performed on a standard sample containing inorganic nitrogen-containing ions by the apparatus of FIG. 1, and FIG. 4 is a chromatogram diagram when sewage was analyzed by the apparatus of FIG. It is a figure. (2) ... Mobile phase storage tank, (3) ... Liquid feed pump, (4) ... Sample inlet, (5) ... Preheating section, (6) ... Cation exchange column, (7) ... … Anion exchange column, (8) …… Electrical conductivity detector, (9) …… Ultraviolet spectrophotometer detector (11) …… Constant temperature chamber, (12) …… Data processing device, (a) …… Analysis flow path.
Claims (3)
を持たないpHが３以上の酸性水溶液を移動相とし、か
つ、陽イオン交換カラムおよび陰イオン交換カラムとを
上記移動相流路に直列に接続したイオンクロマトグラフ
ィに付して上記含窒素イオンを分離し、この分離される
陽イオンフラクションを電気伝導度検出器で、同じく陰
イオンフラクションを紫外吸光検出器でそれぞれ検出す
ることを特徴とする含窒素イオンの一斉分析法。1. A sample containing nitrogen-containing ions is used as a mobile phase in an acidic aqueous solution having no ultraviolet absorption and a pH of 3 or more, and a cation exchange column and an anion exchange column are provided in the mobile phase flow path. The nitrogen-containing ions are separated by subjecting to ion chromatography connected in series, and the separated cation fraction is detected with an electric conductivity detector, and the anion fraction is detected with an ultraviolet absorption detector. Method for simultaneous analysis of nitrogen-containing ions.
ン、アンモニウムイオンからなる群から任意に選択され
た無機含窒素イオンである特許請求の範囲第１項記載の
含窒素イオンの一斉分析法。2. The simultaneous analysis method for nitrogen-containing ions according to claim 1, wherein the nitrogen-containing ions are inorganic nitrogen-containing ions arbitrarily selected from the group consisting of nitrite ion, nitrate ion and ammonium ion. .
液からなる移動相供給部、試料導入口、陽イオン交換カ
ラムおよび陰イオン交換カラムとが直列に接続されたイ
オンクロマトカラム部、電気伝導度検出器および紫外吸
光検出器が直列に接続された含窒素イオン検出部をこの
順に管路接続した分析流路で構成されてなる含窒素イオ
ン一斉分析装置。3. An ion chromatographic column section in which a mobile phase supply section consisting of an acidic aqueous solution having a pH of 3 or more without ultraviolet absorption, a sample inlet, a cation exchange column and an anion exchange column are connected in series, and electricity. A nitrogen-containing ion simultaneous analysis device comprising an analysis flow path in which a nitrogen-containing ion detection section in which a conductivity detector and an ultraviolet absorption detector are connected in series is connected in this order.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30379787A JPH0769312B2 (en) | 1987-11-30 | 1987-11-30 | Simultaneous analysis method for nitrogen-containing ions and its equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30379787A JPH0769312B2 (en) | 1987-11-30 | 1987-11-30 | Simultaneous analysis method for nitrogen-containing ions and its equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01143953A JPH01143953A (en) | 1989-06-06 |
| JPH0769312B2 true JPH0769312B2 (en) | 1995-07-26 |
Family
ID=17925414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP30379787A Expired - Fee Related JPH0769312B2 (en) | 1987-11-30 | 1987-11-30 | Simultaneous analysis method for nitrogen-containing ions and its equipment |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0769312B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103995076A (en) * | 2014-05-20 | 2014-08-20 | 浙江大学 | Method for analyzing and detecting trace ammonia nitrogen in complicated matrix by combining fast distillation method with ion chromatography technology |
| CN106596770B (en) * | 2016-12-13 | 2019-03-29 | 四川大学 | Simultaneously in on-line automatic analysis water-like micro ammonium ion and nitrite ion low pressure anions and canons chromatography-photometry |
| CN109358128B (en) * | 2018-12-03 | 2024-04-09 | 南京大学 | Organic nitrogen-organic carbon serial on-line detection method and device |
| CN111458418B (en) * | 2019-01-22 | 2022-07-19 | 鲁南制药集团股份有限公司 | Method for detecting residual ammonium in enoxaparin sodium |
-
1987
- 1987-11-30 JP JP30379787A patent/JPH0769312B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01143953A (en) | 1989-06-06 |
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