JPH0761949B2 - Composition containing useful enteric bacteria - Google Patents
Composition containing useful enteric bacteriaInfo
- Publication number
- JPH0761949B2 JPH0761949B2 JP2109244A JP10924490A JPH0761949B2 JP H0761949 B2 JPH0761949 B2 JP H0761949B2 JP 2109244 A JP2109244 A JP 2109244A JP 10924490 A JP10924490 A JP 10924490A JP H0761949 B2 JPH0761949 B2 JP H0761949B2
- Authority
- JP
- Japan
- Prior art keywords
- bacteria
- useful
- oil
- squalene
- composition containing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、保存安定性に優れた腸内有用細菌含有組成物
に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial field of use] The present invention relates to a composition containing useful enteric bacteria which is excellent in storage stability.
人間の腸内には多種の細菌類が常在しており、それらの
活動が人間の腸管内の物質の化学反応に関与し、腸の機
能、ひいては人間の健康状態に大きな影響を及ぼすこと
はよく知られている。上記腸内細菌類のうち、ビヒダス
菌や乳酸菌は、それ自体は有害な作用を有せず、その繁
殖によって他の有害な作用を有する細菌類の繁殖を抑制
する働きがある。従って、有害細菌類が原因となる腸内
異常醗酵等による有害な生産物の発生を防止し、健康を
増進させるためには、培養したビヒダス菌や乳酸菌を配
合した製剤や飲食品類を日常的に摂取することが望まし
いとされている。Many kinds of bacteria are resident in the human intestine, and their activities are involved in the chemical reaction of substances in the human intestinal tract, and have a great influence on the function of the intestine and thus on human health. well known. Among the above-mentioned intestinal bacteria, bihidobacteria and lactic acid bacteria do not have a harmful effect by themselves, but have a function of suppressing the reproduction of other bacteria having a harmful effect by their reproduction. Therefore, in order to prevent the generation of harmful products due to abnormal fermentation in the intestine caused by harmful bacteria, and to promote health, in order to improve health, preparations and foods and drinks containing cultured bihidobacteria and lactic acid bacteria are routinely used. It is considered desirable to ingest.
ところが、上記ビヒダス菌や乳酸菌等の腸内有用細菌
は、酸素や水分が存在すると急速に死滅してしまうた
め、大気中で長期間保存することができないという欠点
がある。そのため、上記腸内有用細菌をそのまま、また
は適当な粉末に担持させた状態で油脂中に分散し、酸素
や水分との接触を断つことによって、生菌を長期間安定
に保存しようとする試みがなされてきた。However, the above-mentioned useful bacteria in the intestine, such as the bihidobacteria and lactic acid bacteria, are rapidly killed in the presence of oxygen and water, so that they cannot be stored in the air for a long time. Therefore, the intestinal useful bacteria as they are, or in a state of being supported on a suitable powder, dispersed in fats and oils, by cutting off contact with oxygen and water, there is an attempt to preserve viable bacteria stably for a long period of time. It has been done.
例えば特公昭56−2908号公報には、腸内有用細菌を含有
する乾燥粉末を硬化油、ゴム油、ダイズ油、ツバキ油、
ナタネ油、綿実油等の天然油脂中に懸濁させて安定な生
菌製剤を製造する方法が開示されている。また、特公昭
56−2908号公報には、約35℃以上の温度で流動性を示
し、かつ約30℃以下の温度でほぼ固形状となる油脂、具
体的にはパーム油、ヤシ油あるいはこれらに米ヌカ油、
ナタネ油などを配合した油脂を用いて腸内有用細菌をコ
ーティングする技術が開示されている。また、ビヒダス
菌含有錠菓の製造法に関する特公昭62−39132号公報に
は、ビヒダス菌を錠菓原料に加えるに先立ち、同菌を含
有した粉末を常温で固化している植物性固形脂と混合す
る技術が開示されている。For example, Japanese Examined Patent Publication No. 56-2908 discloses a dry powder containing intestinal useful bacteria hardened oil, rubber oil, soybean oil, camellia oil,
A method for producing a stable viable cell preparation by suspending it in a natural oil or fat such as rapeseed oil or cottonseed oil is disclosed. Also,
No. 56-2908 discloses an oil or fat that exhibits fluidity at a temperature of about 35 ° C. or higher and becomes almost solid at a temperature of about 30 ° C. or lower, specifically palm oil, coconut oil, or rice bran oil. ,
A technique of coating useful enteric bacteria with an oil or fat blended with rapeseed oil or the like is disclosed. In addition, Japanese Patent Publication No. Sho 62-39132 concerning the method for producing bihlidus-containing tablet confectionery, prior to adding bihidas fungus to the raw material for confectionery, a vegetable solid fat solidified at room temperature with a powder containing the fungus. Techniques for mixing are disclosed.
ところが、本発明者は、腸内有用細菌（またはこれを粉
末に担持させたもの）を各種の油脂中に分散させて生菌
の保存安定性を詳細に検討したところ、これまでは生菌
を長期間安定に保存し得るものと考えられていた油脂類
が必ずしもその効果を発揮しないという事実を突き止め
た。そして、本発明者は、さらに各種の物質中に腸内有
用細菌を分散させて生菌の保存安定性を鋭意検討した結
果、驚くべきことに、ある種の油状物質が特異的に腸内
有用細菌を長期間安定に保存する作用を有しているとい
う事実を見出し、本発明に到達したものである。However, the present inventor has conducted a detailed study on the storage stability of live bacteria by dispersing useful bacteria in the intestine (or those which are supported on powder) in various fats and oils, and until now, live bacteria were They found that fats and oils that were thought to be able to be stably stored for a long period of time did not always exert their effects. Then, the present inventor has further studied the storage stability of viable bacteria by dispersing useful enteric bacteria in various substances, and surprisingly, some oily substances are specifically enteric useful. The present invention has been accomplished by finding the fact that it has an action of stably storing bacteria for a long period of time.
本発明の目的は、腸内有用細菌を長期間にわたって安定
に保存することのできる技術を提供することにある。An object of the present invention is to provide a technique capable of stably storing enterobacterially useful bacteria for a long period of time.
本発明は、腸内有用細菌、またはこれを担持した粉末を
スクアレン、スクアランまたはその混合物中に分散させ
た腸内有用細菌含有組成物である。The present invention is a composition containing useful enteric bacteria, wherein intestinal useful bacteria or a powder carrying the same is dispersed in squalene, squalane, or a mixture thereof.
本発明において利用しうる腸内有用細菌としては、B.bi
fidum、B.longum、B.adolescentis、B.breve、B.infant
is等のビヒダス菌（Bifidobacterium）、L.acidophilu
s、L.lactis、L.casei、L.bulgaricus等の乳酸桿菌（La
ctobacillus）およびStreptococcus lactis等を例示す
ることができる。Examples of useful enteric bacteria that can be used in the present invention include B.bi.
fidum, B.longum, B.adolescentis, B.breve, B.infant
Bifidobacterium, such as is, L. acidophilu
s, L. lactis, L. casei, L. bulgaricus, etc.
ctobacillus) and Streptococcus lactis.
本発明の腸内有用細菌含有組成物を調製するには、培養
した生菌を凍結乾燥その他公知の方法によって乾燥粉末
としたもの、あるいはこの乾燥粉末を公知の方法で脱脂
粉乳、蛋白質、澱粉、糖などの生菌保護物質に担持させ
た粉末を上記スクアレンやスクアラン中に配合し、菌
（またはこれを担持した粉末）が液中で均一に分散する
程度に混合攪拌するだけでよい。上記スクアレンやスク
アランは、菌を外気との接触から保護するのに足るだけ
の量を必要とするため、本発明組成物中におけるスクア
レンやスクアランの含有量は、25重量％程度以上である
ことが好ましい。In order to prepare the intestinal useful bacterium-containing composition of the present invention, the cultured live bacteria are freeze-dried by a known method such as freeze-drying, or this dry powder is skimmed milk powder, a protein, a starch, by a known method. It suffices to mix the powder supported by a viable cell-protecting substance such as sugar into the above-mentioned squalene or squalane, and mix and stir until the bacteria (or the powder supporting the same) are uniformly dispersed in the liquid. The above-mentioned squalene and squalane need an amount sufficient to protect the bacteria from contact with the outside air, so the content of squalene and squalane in the composition of the present invention may be about 25% by weight or more. preferable.
本発明組成物が腸内有用細菌を長期間安定に保存する作
用を有する理由については、充分に解明されていない
が、一つの理由として、第２図（相対湿度100％、温度2
5℃の条件下における各種油脂中の水分経時変化を示す
グラフ）に示すように、本発明で用いる例えばスクアレ
ンは、他の油脂類に比べて吸水率の経時変化が極めて小
さいからであると考えられる。The reason why the composition of the present invention has an effect of stably storing useful enteric bacteria for a long period of time has not been sufficiently clarified, but one reason is that FIG. 2 (relative humidity 100%, temperature 2
It is considered that, for example, squalene used in the present invention has an extremely small change with time in water absorption rate as compared with other fats and oils, as shown in a graph showing the change with time in water in various fats and oils under the condition of 5 ° C. To be
以下、実施例により本発明を説明する。Hereinafter, the present invention will be described with reference to examples.
−腸内有用細菌含有組成物の調製− スクアレン75重量部に「ミルフリーズ」（商品名：日
本凍結乾燥研究所製ビヒダス菌原末、生菌濃度1.3×10
10個/g）25重量部を配合し、「ミルフリーズ」の粉末が
スクアレン中に均一に分散するまで攪拌混合した（腸内
有用細菌含有組成物−１）。-Preparation of a composition containing useful bacteria in the intestine- "Mill freeze" was added to 75 parts by weight of squalene.
10 parts / g) was mixed with 25 parts by weight, and the mixture was stirred and mixed until the powder of "Mill freeze" was uniformly dispersed in squalene (composition-1 containing useful enteric bacteria).
スクアレンに代えてスクアランを用いた他は、上記
と同様の処理を行った（腸内有用細菌含有組成物−
２）。The same treatment as described above was performed except that squalane was used instead of squalene (composition containing useful enteric bacteria-
2).
スクアレンに代えてシリコーンオイルを用いた他は、
上記と同様の処理を行った（比較例−１）。Other than using silicone oil instead of squalene,
The same process as above was performed (Comparative Example-1).
スクアレンに代えてサフラワー油を用いた他は、上記
と同様の処理を行った（比較例−２）。The same treatment as described above was performed except that safflower oil was used instead of squalene (Comparative Example-2).
スクアレンに代えて菜種硬化油を用いた他は、上記
と同様の処理を行った（比較例−３）。The same treatment as described above was performed except that hydrogenated rapeseed oil was used instead of squalene (Comparative Example-3).
38℃に加温した液状のカカオ脂75重量部に「ミルフリ
ーズ」25重量部を配合し、「ミルフリーズ」の粉末がカ
カオ脂中に均一に分散するまで攪拌混合した（比較例−
４）。75 parts by weight of liquid cocoa butter heated to 38 ° C was mixed with 25 parts by weight of "Mill freeze", and the mixture was stirred and mixed until the powder of "Mill freeze" was uniformly dispersed in the cocoa butter (Comparative Example-
4).
−保存試験− 上記腸内有用細菌含有組成物1,2および比較例組成物１
〜４のそれぞれを温度25℃、相対湿度100％の環境下で
保存し、各組成物中のビヒダス菌数の経時変化を測定し
た。試験結果を第１図に示す。—Preservation test— Compositions 1 and 2 containing intestinal useful bacteria and Composition 1 of Comparative Example
Each of Nos. 4 to 4 was stored in an environment of a temperature of 25 ° C. and a relative humidity of 100%, and the time-dependent change in the number of Vihydus bacteria in each composition was measured. The test results are shown in FIG.
上記試験結果から明らかなように、比較例組成物１〜４
中のビヒダス菌数は、数日で急速に減少したのに対し、
腸内有用細菌含有組成物1,2中のビヒダス菌数は、１週
間経過後も殆ど変化せず、優れた保存安定性を示した。As is clear from the above test results, Comparative Compositions 1-4
While the number of Vihidus bacteria in the population decreased rapidly in a few days,
The number of Bihidus bacteria in the intestinal useful bacteria-containing compositions 1 and 2 showed almost no change even after 1 week, showing excellent storage stability.
腸内有用細菌、または前記腸内有用細菌を担持した粉末
をスクアレンやスクアラン中に分散させた本発明の腸内
有用細菌含有組成物によれば、大気中で死滅し易い脹内
有用細菌を長期間安定に保存することができる。また、
本発明の腸内有用細菌含有組成物は液状であるため、移
送、分取が容易であり、カプセル充填等も容易に行うこ
とができる。According to the intestinal useful bacterium-containing composition of the present invention in which the intestinal useful bacterium or the powder carrying the intestinal useful bacterium is dispersed in squalene or squalane, the intestinal useful bacterium can be easily killed in the atmosphere. It can be stored stably for a period of time. Also,
Since the intestinal useful bacterium-containing composition of the present invention is in a liquid state, it can be easily transferred and fractionated, and can be easily filled into capsules.
第１図は、本発明の腸内有用細菌含有組成物中および比
較例組成物中におけるビヒダス菌数の経時変化をそれぞ
れ示すグラフ図、 第２図は、油脂中の水分の経時変化を示すグラフ図であ
る。FIG. 1 is a graph showing changes over time in the number of bihidobacteria in the intestinal useful bacterium-containing composition of the present invention and in the comparative composition, and FIG. 2 is a graph showing changes over time in water content in fats and oils. It is a figure.
Claims (1)
担持した粉末をスクアレン、スクアランまたはその混合
物中に分散させてなる腸内有用細菌含有組成物。1. A composition containing useful enteric bacteria, which comprises dispersing the enteric useful bacteria or a powder carrying the enteric useful bacteria in squalene, squalane, or a mixture thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2109244A JPH0761949B2 (en) | 1990-04-25 | 1990-04-25 | Composition containing useful enteric bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2109244A JPH0761949B2 (en) | 1990-04-25 | 1990-04-25 | Composition containing useful enteric bacteria |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61043651A Division JPS62201823A (en) | 1986-02-28 | 1986-02-28 | Material containing beneficial enterobacterium and production thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0395122A JPH0395122A (en) | 1991-04-19 |
| JPH0761949B2 true JPH0761949B2 (en) | 1995-07-05 |
Family
ID=14505271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2109244A Expired - Lifetime JPH0761949B2 (en) | 1990-04-25 | 1990-04-25 | Composition containing useful enteric bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0761949B2 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK242083D0 (en) * | 1983-05-27 | 1983-05-27 | Hansens Chr Bio Syst | vaginal |
| JPS61151127A (en) * | 1984-12-25 | 1986-07-09 | Meiji Milk Prod Co Ltd | Production of soft capsule containing bifidus bacteria |
-
1990
- 1990-04-25 JP JP2109244A patent/JPH0761949B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0395122A (en) | 1991-04-19 |
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