JPH0758285B2 - Simultaneous analysis of anions and cations - Google Patents
Simultaneous analysis of anions and cationsInfo
- Publication number
- JPH0758285B2 JPH0758285B2 JP1043918A JP4391889A JPH0758285B2 JP H0758285 B2 JPH0758285 B2 JP H0758285B2 JP 1043918 A JP1043918 A JP 1043918A JP 4391889 A JP4391889 A JP 4391889A JP H0758285 B2 JPH0758285 B2 JP H0758285B2
- Authority
- JP
- Japan
- Prior art keywords
- cations
- anions
- exchange column
- cation
- anion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Description
【発明の詳細な説明】 （イ） 産業上の利用分野 本発明は，上水，下水，排水などの各種水，生体試料，
食品，医薬品，化学合成品などに含まれる陰イオン及び
陽イオンの同時分析法に関する。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention is directed to various water such as tap water, sewage, drainage, biological samples,
The present invention relates to a simultaneous analysis method for anions and cations contained in foods, pharmaceuticals, chemically synthesized products and the like.
（ロ） 従来技術 従来，陰イオンと陽イオンは，イオンクロマトグラフィ
ーにより各々別々のカラムおよび移動相を用いて分析さ
れており（例えば，特公昭56-23100号公報,J.Chromatog
r.vol176,P199（1979年）），イオンクロマトの分野で
は陰イオンと陽イオンを同時に分析した例はなかった。(B) Conventional technology Conventionally, anions and cations have been analyzed by ion chromatography using separate columns and mobile phases (see, for example, Japanese Examined Patent Publication No. Sho 56-23100, J. Chromatog).
r.vol176, P199 (1979)), in the field of ion chromatography, there was no example of simultaneous analysis of anions and cations.
なお，陰イオンと陽イオンを同時に分析する方法とし
て，イオンの種別毎に感応するイオン選択性電極を複数
個用意して行うものがわずかに存在するだけである。There are only a few methods for simultaneously analyzing anions and cations by preparing a plurality of ion-selective electrodes sensitive to each type of ion.
（ハ） 発明が解決しようとする課題 上記のように，イオンクロマトグラフィーで陰イオンと
陽イオンを分析しようと欲すれば各々別々のカラムおよ
び移動相を用意しなければならないので，その交換に手
間どり分析時間が長くなった。(C) Problems to be Solved by the Invention As described above, if one wishes to analyze anions and cations by ion chromatography, separate columns and mobile phases must be prepared for each. Analysis time became longer.
また，イオン選択性電極による分析法は，イオンの種別
毎に電極を用意しなければならないので，測定対象が複
数種のイオンに亘る場合不可能に近かった。Further, the analysis method using the ion-selective electrode is almost impossible because the electrode must be prepared for each type of ion, so that the measurement target covers a plurality of types of ions.
本発明は，上記の事情に鑑みなされたもので，陰イオン
と陽イオンをイオンクロマトグラフィーにより同時に分
析することを目的とするものである。The present invention has been made in view of the above circumstances, and an object thereof is to simultaneously analyze anions and cations by ion chromatography.
（ニ） 課題を解決するための手段 本件発明者は，鋭意検討の結果フタル酸とトリス（ヒド
ロキシメチル）アミノメタンの混合液からなる移動相を
特定の濃度,pH範囲内で使用すれば陰イオンと陽イオン
を同時に分析できることを見出し本発明に至ったもので
ある。(D) Means for Solving the Problems As a result of intensive studies, the inventors of the present invention have found that if a mobile phase consisting of a mixed solution of phthalic acid and tris (hydroxymethyl) aminomethane is used within a specific concentration and pH range. The present invention has led to the present invention by discovering that cations and cations can be analyzed simultaneously.
すなわち，その具体的構成は，移動相としてpH4.7〜5.
2,濃度1.5mM〜2.0mMの範囲内にあるフタル酸とトリス
（ヒドロキシメチル）アミノメタンの混合液を用いて，
陰・陽イオン含有試料を陽イオン交換カラム及び陰イオ
ン交換カラムに順に付し，陰・陽イオンを各々分離した
後電気伝導度検出器により検出することを特徴とするも
のである。That is, the specific composition of the mobile phase is pH 4.7 to 5.
2, using a mixture of phthalic acid and tris (hydroxymethyl) aminomethane in a concentration range of 1.5 mM to 2.0 mM,
It is characterized in that the sample containing anions and cations is sequentially applied to a cation exchange column and an anion exchange column, and the anions and cations are separated and then detected by an electric conductivity detector.
なお，移動相のpHが4.7以下では，システムピークのト
リスイオンが陰イオンに重なりpHが,5.2以上になるとシ
ステムピークのフタル酸イオンが陽イオンに重なる。When the pH of the mobile phase is below 4.7, the tris ion at the system peak overlaps with the anion, and at pH above 5.2, the phthalate ion at the system peak overlaps with the cation.
また，移動相の濃度を1.5mM以下にすると分析時間が長
くなり,2.0mM以上にするとNO- 3,SO4 2-が分離しない。Further, the concentration of the mobile phase increases analysis time and to 1.5mM below, when more than 2.0mM NO - 3, SO 4 2- are not separated.
（ホ） 作用 本発明では，移動相条件の特定により陽イオン交換カラ
ムからのシステムピークを陰イオン（Cl-）の前に溶出
させ，陰イオン交換カラムからのシステムピークを陽イ
オン（K+）の後に溶出させ，陰イオンと陽イオンの同時
分析を可能とする。(E) Action In the present invention, the system peak from the cation exchange column is eluted before the anion (Cl − ) by specifying the mobile phase conditions, and the system peak from the anion exchange column is converted into the cation (K + ). It is eluted after and enables simultaneous analysis of anions and cations.
（ヘ） 実施例 まず，本発明に係る方法を実施するための装置の基本的
構成を第１図に示す。(F) Example First, FIG. 1 shows a basic configuration of an apparatus for carrying out the method according to the present invention.
1,1′は移動相液溜,2は送液ポンプ,3は陽イオン用ガー
ドカラム,4は陽イオン交換カラム,5は陰イオン交換カラ
ム,6は電気伝導度検出器,7は恒温槽である。1, 1'is a mobile phase liquid reservoir, 2 is a liquid feed pump, 3 is a cation guard column, 4 is a cation exchange column, 5 is an anion exchange column, 6 is a conductivity detector, and 7 is a constant temperature bath. Is.
かかる構成のもと，次の分析条件により分析したクロマ
トグラムが第２図である。FIG. 2 shows a chromatogram analyzed under the following conditions under such a constitution.
試料:NaCl,NaNO2,NaBr,NaNO3，Na2SO4混合溶液（各陰イ
オン10ppm） NaCl,NH4Cl,KCl混合溶液（各陽イオン10ppm） 移動相:17mM−フタル酸・トリス（ヒドロキシメチル）
アミノメタン混合液 （pH4.94） 移動相流量:1.3ml/min ガードカラム:Shim-pack IC-GCl（島津製作所製）i.d.8
mmφ×5cm 陽イオン交換カラム:Shim-pack IC−ｌ（島津製作所
製）i.d.5.0mmφ×15cm 陰イオン交換カラム:Shim-pack IC-Al（島津製作所製）
i.d.4.6mmφ×10cm 恒温槽温度:40℃ 検出器：電気伝導度検出器CDD-6A（島津製作所製） 第２図より，各種陰イオン（Cl-,NO2 -,NO3 -,SO4 2-）と
各種陽イオン（Na+,NH4 +，K+）のピーク及び移動相由来
のシステムピーク・トリス（ヒドロキシメチル）アミノ
メタンとフタル酸イオンが相互に重ならずに出てくるこ
とがわかる。Sample: NaCl, NaNO 2 , NaBr, NaNO 3 , Na 2 SO 4 mixed solution (each anion 10 ppm) NaCl, NH 4 Cl, KCl mixed solution (each cation 10 ppm) Mobile phase: 17 mM-phthalic acid / tris (hydroxy) Methyl)
Aminomethane mixture (pH4.94) Mobile phase flow rate: 1.3 ml / min Guard column: Shim-pack IC-GCl (manufactured by Shimadzu) id8
mmφ × 5cm Cation exchange column: Shim-pack IC-1 (Shimadzu) id 5.0mmφ × 15cm Anion exchange column: Shim-pack IC-Al (Shimadzu)
id4.6mmφ × 10cm thermostatic bath temperature: 40 ° C. Detector: from Figure 2 the electrical conductivity detector CDD-6A (manufactured by Shimadzu Corporation), various anions (Cl -, NO 2 -, NO 3 -, SO 4 2 - ) And various cations (Na + , NH 4 + , K + ) peaks and mobile phase derived system peaks-tris (hydroxymethyl) aminomethane and phthalate ions may appear without overlapping each other. Recognize.
なお，上記説明では，陽イオン交換カラムを陰イオン交
換カラムの前に接続したが，この接続順序はどちらでも
良い。In the above description, the cation exchange column was connected before the anion exchange column, but this connection order does not matter.
（ト） 効果 本発明によれば，イオンクロマトグラフィーにより陽イ
オンと陰イオンを同時に分析できる。(G) Effect According to the present invention, cations and anions can be analyzed simultaneously by ion chromatography.
第１図は，本発明に係る方法を実施するための装置構成
図，第２図は，本発明の方法により陽イオン・陰イオン
混合物を分析したときのクロマトグラムである。 ４……陽イオン交換カラム ５……陰イオン交換カラムFIG. 1 is an apparatus configuration diagram for carrying out the method according to the present invention, and FIG. 2 is a chromatogram when a cation / anion mixture is analyzed by the method of the present invention. 4 …… Cation exchange column 5 …… Anion exchange column
Claims (1)
Mの範囲内にあるフタル酸とトリス（ヒドロキシメチ
ル）アミノメタンの混合液を用いて，陰・陽イオン含有
試料を陽イオン交換カラム及び陰イオン交換カラムに順
に付し，陰・陽イオンを各々分離した後電気伝導度検出
器により検出することを特徴とする陰イオン及び陽イオ
ンの同時分析法。1. A mobile phase having a pH of 4.7 to 5.2 and a concentration of 1.5 mM to 2.0 m
Using a mixed solution of phthalic acid and tris (hydroxymethyl) aminomethane in the range of M, anion- and cation-containing samples were sequentially applied to a cation-exchange column and an anion-exchange column, respectively. A method for simultaneous analysis of anions and cations, which is characterized by separating and then detecting with an electric conductivity detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1043918A JPH0758285B2 (en) | 1989-02-23 | 1989-02-23 | Simultaneous analysis of anions and cations |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1043918A JPH0758285B2 (en) | 1989-02-23 | 1989-02-23 | Simultaneous analysis of anions and cations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02223857A JPH02223857A (en) | 1990-09-06 |
| JPH0758285B2 true JPH0758285B2 (en) | 1995-06-21 |
Family
ID=12677094
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1043918A Expired - Lifetime JPH0758285B2 (en) | 1989-02-23 | 1989-02-23 | Simultaneous analysis of anions and cations |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0758285B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2700986B2 (en) * | 1992-12-07 | 1998-01-21 | 吉田 明浩 | Method for simultaneous determination of multiple inorganic acids |
| JP2002372521A (en) * | 2001-06-14 | 2002-12-26 | Tosoh Corp | Measuring method of alkalinity using ion chromatograph method and simultaneous measuring method with monovalent cation |
-
1989
- 1989-02-23 JP JP1043918A patent/JPH0758285B2/en not_active Expired - Lifetime
Non-Patent Citations (2)
| Title |
|---|
| Ｊ．Ｃｈｖｏｍａｔｏｇｒ．，４１１，（１９８７），ｐ１０７−１９ |
| Ｍａｇｙ．Ｋｅｎ．Ｆｏｌｙ．，９４（４），（１９８８）．ｐ１５８−６２ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02223857A (en) | 1990-09-06 |
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