JPH0751051A - New fine algae and method for fixing highly concentrated carbon dioxide with the same - Google Patents
New fine algae and method for fixing highly concentrated carbon dioxide with the sameInfo
- Publication number
- JPH0751051A JPH0751051A JP5203118A JP20311893A JPH0751051A JP H0751051 A JPH0751051 A JP H0751051A JP 5203118 A JP5203118 A JP 5203118A JP 20311893 A JP20311893 A JP 20311893A JP H0751051 A JPH0751051 A JP H0751051A
- Authority
- JP
- Japan
- Prior art keywords
- carbon dioxide
- strain
- chlorococcum
- highly concentrated
- new fine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treating Waste Gases (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、空気中のそれよりもは
るかに高い濃度の二酸化炭素を含むガスを付加して培養
を行っても活発に分裂増殖する新規海産微細藻類クロロ
コックム ドルシベントラレ（Chlorococcum dorsivent
rale）、及び、それを用いて排ガス中の二酸化炭素を固
定する方法に関する。The present invention relates, even if the culture by adding a gas containing carbon dioxide much higher concentration than that in the air actively dividing proliferating new marine microalgae Kurorokokkumu Dorushibentorare (Chlorococcum dorsivent
rale ) and a method for fixing carbon dioxide in exhaust gas using the same.
【０００２】[0002]
【従来の技術】通常、微細藻類（特に緑藻）の培養にお
いては、増殖速度を上げるために５％程度の二酸化炭素
を含むガスを付加していたが、それ以上の濃度の二酸化
炭素を含むガスを用いて緑藻を培養している例はなかっ
た。また、微細藻類を用いて有用物質を生産したりバイ
オマスを生産した例は数多くあるが、太平洋の外洋域か
ら新規に微細藻類を分離しその株によって積極的に排ガ
ス中に含まれる高濃度二酸化炭素を固定しようとする方
法はなかった。2. Description of the Related Art Usually, in culturing microalgae (particularly green algae), a gas containing carbon dioxide of about 5% is added to increase the growth rate. There was no case of culturing green algae using. In addition, there are many examples of producing useful substances and biomass using microalgae, but new microalgae were newly separated from the open ocean of the Pacific Ocean, and the high concentration of carbon dioxide contained in the exhaust gas was positively contained by the strain. There was no way to try to fix.
【０００３】[0003]
【発明が解決しようとする課題】本発明の目的は、広く
太平洋の外洋域の海水を採取し、その中から新規な微細
藻類株を見いだし、その中からさらに高濃度二酸化炭素
に耐性のある株をスクリーニングし、得られた微細藻類
株を用いて増殖の条件を検討し、効率よく排ガス中の二
酸化炭素を固定する方法を開発することにある。DISCLOSURE OF THE INVENTION The object of the present invention is to collect seawater from a wide area of the open ocean of the Pacific Ocean, to find out a novel microalgae strain from the seawater, which is resistant to a higher concentration of carbon dioxide. The purpose of the present invention is to develop a method for efficiently fixing carbon dioxide in exhaust gas by screening the above, examining the growth conditions using the obtained microalgae strain.
【０００４】[0004]
【課題を解決しようとする手段】本発明は、太平洋外洋
域から得られた高濃度の二酸化炭素に耐性を有する新規
微細藻クロロコックム ドルシベントラレ（Chlorococc
um dorsiventrale）にある。また、本発明は太平洋外洋
域から得られた高濃度二酸化炭素に耐性を有する新規微
細藻クロロコックム ドルシベントラレ クラノ エト
チハラ エスピーノブ（Chlorococcum dorsiventrale
Kurano et Chihara sp. nov. ）株にある。DISCLOSURE OF THE INVENTION The present invention provides a novel microalga Chlorococcus dulcibentrale ( Chlorococca), which is resistant to high concentrations of carbon dioxide obtained from the Pacific ocean.
um dorsiventrale ). In addition, the present invention is a novel microalga, Chlorococcum dorsiventrale ( Chlorococcum dorsiventrale), which is resistant to high concentration carbon dioxide obtained from the Pacific Ocean.
Kurano et Chihara sp. Nov.) Strain.
【０００５】さらに、本発明は排ガス中の二酸化炭素を
固定する方法において、新規微細藻類クロロコックム
ドルシベントラレ（Chlorococcum dorsiventrale）を使
用することを特徴とする二酸化炭素固定法にある。以
下、本発明を詳細に説明する。発明者らは、広く太平洋
外洋域の海水を採取しメンブランフィルターにて海水中
に含まれる微細藻類を濃縮した。フィルター上に濃縮さ
れた微細藻類を表１に示すＭＣ培地を１０ｍｌ入れた試
験管にフィルターごと移し、そこへ連続的に光を照射し
ながら２０％二酸化炭素を通気した。Further, the present invention relates to a novel microalgae chlorococcum in a method of fixing carbon dioxide in exhaust gas.
A carbon dioxide fixing method characterized by using Dorciventrare ( Chlorococcum dorsiventrale ). Hereinafter, the present invention will be described in detail. The inventors widely collected seawater in the Pacific ocean and concentrated the microalgae contained in the seawater with a membrane filter. The microalgae concentrated on the filter were transferred together with the filter to a test tube containing 10 ml of the MC medium shown in Table 1, and 20% carbon dioxide was aerated while continuously irradiating with light.
【０００６】[0006]
【表１】 [Table 1]
【０００７】その結果、西太平洋グアム島沖外洋域の海
水サンプルから２０％二酸化炭素通気条件下で活発に生
育する新規微細藻クロロコックム ドルシベントラレ
クラノ エト チハラ エスピー ノブ（Chlorococcum
dorsiventrale Kurano et Chihara sp. nov. ）（以
下、本株と略す）を単離した。なお、本株は（株）海洋
バイオテクノロジー研究所において保存番号26-0/8等と
して保存管理されている。As a result, a novel microalga, Chlorococcum driciventrare, which actively grows from a seawater sample off the coast of Guam in the western Pacific Ocean under aeration conditions of 20% carbon dioxide.
Kuranoet Chihara Espnobu ( Chlorococcum
dorsiventrale Kurano et Chihara sp. nov.) (hereinafter abbreviated as this strain) was isolated. This strain is stored and managed by Marine Biotechnology Research Institute Co., Ltd. under the storage number 26-0 / 8.
【０００８】本株の藻類学的性質は次の通りである。 １．形態的性質 （１）栄養細胞の体は単細胞で緑色をしている。細胞は
球形ないしやや楕円形に近い球形である。 （２）栄養細胞の体は培養の経過とともに直径２．５−
１３μｍの範囲で容積を増大させる。 （３）厚さ０．１−０．２μｍの細胞壁を有する。壁の
周囲には寒天状の物質は認められない。 （４）葉緑体は一個で杯状である。ピレノイド及び核は
ともに一個である。ピレノイドの中に葉緑体チラコイド
膜が貫通しており、ピレノイドの周囲には２枚ないし数
枚の時計皿状のでんぷんをつける。 （５）一細胞中に４、８、１２、１６、３２の遊走子あ
るいは自生胞子をつくる。この場合には、一細胞中の葉
緑体、核、ピレノイドの数は胞子の数に等しい。 （６）遊走子は左右非相称で等長の２本の鞭毛を有す
る。すなわち、鞭毛基部を頭とすると腹と背が識別され
る（図１）。この点で既知のクロロコックム リトラレ
（Chlorococcum littorale）と明白に異なっている。 （７）遊走子は基質に付着した後、しばらくは原形を保
つ。すなわち、細胞壁を有している。 （８）遊走子の前端が接合する有性生殖が観察される。 ２．生理学的性状 （１）集積培養：組成中にカルシウムを含まないＭＣ培
地を用いた場合に、特徴的に集積される。すなわち、こ
の藻はカルシウム要求性が低い。 （２）培養：海水ベース、人工海水ベースのいずれの培
地においても良好な生育を示すが淡水ベースの培地にお
いては著しい生育低下が認められる。 （３）光合成能：光独立栄養的に生育可能である。貯蔵
物質としてでんぷんを蓄積する。 （４）光合成色素：クロロフィルａ、クロロフィルｂ、
ルテイン、ヴィオラキサンティン、ゼアキサンティンを
含み、典型的な緑藻の色素組成を示す。 （５）生育温度域：１５〜３０℃。 （６）生育ｐＨ域：ｐＨ４〜９。 （７）生育ＮａＣｌ濃度域：１〜１０％。 ３．属及び種の決定 本株は、光合成色素としてクロロフィルａ、クロロフィ
ルｂ、ルテイン、ヴィオラキサンティン、ゼアキサンテ
ィンを含んでおり、典型的な緑藻のパターンを示す。ま
た、上述の形態的性質は、緑藻網（Chlorophycea）、ク
ロロコックム目（Chlorococcales）、クロロコックム属
（Chlorococcum）と完全に一致する。従って、本株はク
ロロコックム属の株である。The algal properties of this strain are as follows. 1. Morphological properties (1) The body of vegetative cells is a single cell and has a green color. The cells are spherical or spherical with a shape close to an ellipse. (2) The body of vegetative cells has a diameter of 2.5-
The volume is increased in the range of 13 μm. (3) It has a cell wall with a thickness of 0.1 to 0.2 μm. No agar-like material is found around the walls. (4) One chloroplast is cup-shaped. There are only one pyrenoid and one nucleus. The chloroplast thylakoid membrane penetrates through the pyrenoid, and two or several pieces of watch glass-like starch are attached around the pyrenoid. (5) Produce 4, 8, 12, 16, 32 zoospores or autospores in one cell. In this case, the number of chloroplasts, nuclei, and pyrenoids in one cell is equal to the number of spores. (6) The zoospores are bilaterally asymmetric and have two flagella of equal length. That is, when the flagella base is the head, the belly and the back are distinguished (Fig. 1). This is clearly different from the known Chlorococcum littorale . (7) The zoospores maintain their original shape for a while after attaching to the substrate. That is, it has a cell wall. (8) Sexual reproduction in which the front ends of zoospores are joined is observed. 2. Physiological properties (1) Accumulation culture: Characteristically accumulated when an MC medium containing no calcium in the composition is used. That is, this alga has a low calcium requirement. (2) Cultivation: Good growth is shown in both seawater-based and artificial seawater-based media, but a marked decrease in growth is observed in freshwater-based media. (3) Photosynthetic ability: It can grow photoautotrophically. Accumulates starch as a storage material. (4) Photosynthetic pigments: chlorophyll a, chlorophyll b,
It contains lutein, violaxanthin, and zeaxanthin, and shows a typical pigment composition of green algae. (5) Growth temperature range: 15 to 30 ° C. (6) Growth pH range: pH 4-9. (7) Growth NaCl concentration range: 1 to 10%. 3. Genus and species determination This strain contains chlorophyll a, chlorophyll b, lutein, violaxanthin, and zeaxanthin as photosynthetic pigments, and shows a typical green alga pattern. Also, morphological properties described above, the green alga network (Chlorophycea), chlorococcales (Chlorococcales), all the same with Kurorokokkumu genus (Chlorococcum). Therefore, this strain is a chlorococcum strain.
【０００９】現在までに、海産のクロロコックム属はク
ロロコックム サブマリヌム（Chlorococcum submarinu
m）、とクロロコックム リフトラレ（Chlorococcum li
ftorale）が知られている。本株は、細胞壁の厚さと細
胞の外周に寒天上の物質を持たない点でC.submarinumと
異なっており、また、遊走子の形態の点で明白にC.lift
oraleと異なっている。以上の点を考慮して、２０％二
酸化炭素通気条件下で活発に生育する本株をクロロコッ
クム ドルシベントラレ クラノ エト チハラ エス
ピー ノブ（Chlorococcum dorsiventrale Kurano et C
hihara sp. nov. ）と命名した。なお、種名は遊走子の
特徴的な形態、すなわち、背と腹が区別される背腹性を
有している点にちなんでいる。To date, the marine genus Chlorococcum is known as Chlorococcum submarinu.
m ), and Chlorococcum li ( Chlorococcum li
ftorale ) is known. This strain differs from C. submarinum in the thickness of the cell wall and the absence of substances on the agar around the cells, and the morphology of zoospores clearly shows that C. lift.
Different from orale . Considering the above points, this strain, which actively grows under the condition of 20% carbon dioxide aeration, was added to Chlorococcum dorsiventrale Kurano et Shiranobu ( Chlorococcum dorsiventrale Kurano et C
hihara sp. nov.). The species name is named after having a characteristic form of zoospores, that is, having dorsoventral characteristics in which the back and the abdomen are distinguished.
【００１０】以下に、二酸化炭素固定の観点から見て重
要である増殖特性について述べる。 １．二酸化炭素濃度 本株は、図２に示すように空気レベルから６０％の分圧
の二酸化炭素濃度まで生育可能である。また、２０％の
分圧の場合、ほとんど生育阻害を受けない。これは、排
ガスに含まれる程度の二酸化炭素の濃度では、生育速度
が低下せず十分な二酸化炭素固定能力を発揮できること
を示している。 ２．ｐＨ 本株は、図３に示すようにｐＨ４から９の広い範囲で生
育可能である。２０％二酸化炭素を微細藻培養液に吹き
込んだ場合、緩衝作用の強い海水をベースにした培地の
場合でもｐＨが４程度まで低下してしまう。このような
場合でも、本株を用いることにより、効率的な二酸化炭
素固定が可能である。 ３．ＮａＣｌ濃度 図４に示すように本株は、淡水を基本とした培地では生
育できない。しかし、その培地に、ＮａＣｌを１％添加
すると海水の場合と同等以上の生育速度を示す。さら
に、最終藻体濃度や生育速度は低下するがＮａＣｌを１
０％添加した場合でも生育する。この点は、屋外におけ
る粗放的な培養システムを考えた場合に非常に有利な点
である。大量の排ガスを処理するためには、なるべく手
間のかからない粗放的な方法も望ましい手段の一つの選
択肢である。屋外での培養池による二酸化炭素処理シス
テムの場合、培養の経過に伴って水分の蒸発による培養
液中の塩濃度が上昇しそれによって惹起される増殖阻害
が問題となるが、本株の場合、株自身が高塩濃度耐性を
有しており特にその問題に対する対策を考慮する必要が
ない。この点でも、二酸化炭素固定に適した性質を有し
ている。The growth characteristics which are important from the viewpoint of carbon dioxide fixation will be described below. 1. Carbon Dioxide Concentration This strain can grow from the air level to a carbon dioxide concentration of 60% partial pressure as shown in FIG. Further, when the partial pressure is 20%, there is almost no growth inhibition. This indicates that at a concentration of carbon dioxide contained in the exhaust gas, the growth rate does not decrease and a sufficient carbon dioxide fixing ability can be exhibited. 2. pH This strain can grow in a wide range of pH 4 to 9, as shown in FIG. When 20% carbon dioxide is blown into the microalgae culture solution, the pH drops to about 4 even in the case of a seawater-based medium having a strong buffering effect. Even in such a case, the use of this strain enables efficient carbon dioxide fixation. 3. NaCl concentration As shown in FIG. 4, this strain cannot grow in a medium based on fresh water. However, when 1% of NaCl is added to the medium, the growth rate is equal to or higher than that of seawater. Furthermore, although the final algal cell concentration and growth rate decrease, NaCl
It grows even when 0% is added. This is a very advantageous point when considering a loose culture system outdoors. In order to treat a large amount of exhaust gas, a bleaching method that is the least troublesome is also a desirable option. In the case of a carbon dioxide treatment system using an outdoor culture pond, the concentration of salt in the culture solution due to the evaporation of water increases with the progress of culture and the growth inhibition caused by it becomes a problem, but in the case of this strain, Since the strain itself has high salt concentration tolerance, it is not necessary to consider measures against the problem. Also in this respect, it has a property suitable for carbon dioxide fixation.
【００１１】[0011]
【実施例】以下、実施例により本発明を説明する。ただ
し、本発明の技術的範囲は実施例によりなんら限定され
るものではない。 （実施例１）内径１４０ｍｍ、高さ３００ｍｍの透明円
筒形ガラス製培養槽に岩手県釜石湾から採取した海水を
ベースとした栄養塩培地（ＭＣ培地、表１に組成を示
す）を４リットルいれて、オートクレーブ滅菌したもの
に本株の前培養液１００ｍｌを添加し、培養を開始し
た。培養槽の回りからサークライン蛍光灯によって断続
的に光を供給し、温度は２５℃、通気量は０．２５ｖｏ
ｌ／ｖｏｌ／ｍｉｎ、攪拌速度２００ｒｐｍを保って培
養した。この培養条件で、通気するガス中の二酸化炭素
分圧を０〜７０％まで変化させた時の増殖の経過を図２
に示す。本株は、５％、２０％の二酸化炭素分圧におい
ても活発に分裂増殖する能力を有した。EXAMPLES The present invention will be described below with reference to examples. However, the technical scope of the present invention is not limited to the examples. (Example 1) Into a transparent cylindrical glass culture tank having an inner diameter of 140 mm and a height of 300 mm, add 4 liters of a nutrient salt medium (MC medium, the composition of which is shown in Table 1) based on seawater collected from Kamaishi Bay, Iwate Prefecture. Then, 100 ml of the preculture liquid of this strain was added to the autoclave-sterilized product to start the culture. Light is intermittently supplied from around the culture tank by a circline fluorescent lamp, the temperature is 25 ° C, and the aeration is 0.25vo.
The culture was performed at 1 / vol / min and a stirring speed of 200 rpm. Under this culture condition, the progress of growth when the partial pressure of carbon dioxide in the gas to be aerated is changed from 0 to 70% is shown in FIG.
Shown in. This strain had the ability to actively divide and grow even at a carbon dioxide partial pressure of 5% and 20%.
【００１２】（実施例２）実施例１と同じ培養装置と条
件で、本株の二酸化炭素固定能力を測定した。本株は、
２０％二酸化炭素通気条件下で、対数増殖期の倍加時間
が８時間、直線増殖期の生育速度が１リットルの培養液
あたり１日あたり０．５グラムの乾燥重量増、最高到達
藻体濃度が１リットルあたり４グラム乾燥重量という高
い生育能力を示した。そして、直線増殖期の二酸化炭素
固定能力は、に示すように約１リットルの培養液あたり
１日あたり０．７３グラム二酸化炭素であった。直線増
殖期は図に示すように約２０日間続いたので、トータル
の二酸化炭素固定量は１リットルあたり約１５グラムで
あった。(Example 2) The carbon dioxide-fixing ability of this strain was measured under the same culture apparatus and conditions as in Example 1. This strain is
Under aerated condition of 20% carbon dioxide, the doubling time in the logarithmic growth phase is 8 hours, the growth rate in the linear growth phase is 0.5 g per day, and the dry weight increase is 0.5 g per day. It showed a high viability of 4 g dry weight per liter. Then, the carbon dioxide-fixing ability in the linear growth phase was 0.73 g carbon dioxide per day per about 1 liter of the culture solution as shown in. Since the linear growth phase continued for about 20 days as shown in the figure, the total fixed amount of carbon dioxide was about 15 grams per liter.
【００１３】（実施例３）内径１８ｍｍの試験管に１０
ｍｌの淡水ベースのＭＣ培地を入れて、そこにＮａＣｌ
を０〜１０％添加して本株を培養した。培養条件は、実
施例１に準じた。結果を図４に示す。本株は、海水の代
わりにＮａＣｌを添加した培地での生育が可能であった
が、ＮａＣｌの濃度が０の場合ほとんど生育しなかっ
た。また、海水よりも高いＮａＣｌ濃度においても最高
時の半分程度の藻体濃度まで到達した。(Embodiment 3) Ten test tubes each having an inner diameter of 18 mm are used.
Add ml of fresh water-based MC medium and add NaCl to it.
This strain was cultivated by adding 0 to 10%. The culture conditions were the same as in Example 1. The results are shown in Fig. 4. This strain was able to grow in a medium to which NaCl was added instead of seawater, but it hardly grew when the NaCl concentration was 0. Further, even at a NaCl concentration higher than that of seawater, the concentration of algal cells reached about half of the maximum.
【００１４】[0014]
【発明の効果】本株は、高濃度二酸化炭素耐性、低ｐＨ
耐性、高塩濃度耐性のいずれをも兼ね備えており、他の
微細藻類に比べて排ガス中の二酸化炭素固定に適してい
る。本株を用いることにより、粗放的なシステムによる
排ガス中二酸化炭素処理が可能となる。[Effect of the Invention] This strain has high carbon dioxide tolerance and low pH.
It has both tolerance and high salt concentration tolerance, and is more suitable for carbon dioxide fixation in exhaust gas than other microalgae. By using this strain, it becomes possible to treat carbon dioxide in exhaust gas by a coarse discharge system.
【図１】 本株の特徴である遊走子の形態を示す透過電
子顕微鏡写真FIG. 1 Transmission electron micrograph showing morphology of zoospores, which is a characteristic of this strain.
【図２】 本株の各二酸化炭素濃度における増殖の経過[Fig. 2] Progress of growth of this strain at various carbon dioxide concentrations
【図３】 本株の各ｐＨにおける培養２週間後の増殖量FIG. 3 Growth amount of this strain after 2 weeks of culture at each pH
【図４】 本株の各ＮａＣｌ濃度における培養２週間後
の増殖量FIG. 4 Growth amount of this strain after 2 weeks of culture at each NaCl concentration
フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ１２Ｒ 1:89） Continuation of front page (51) Int.Cl. 6 Identification code Office reference number FI technical display area C12R 1:89)
Claims (3)
細藻類クロロコックム ドルシベントラレ（Chlorococc
um dorsiventrale）。1. A novel microalga, Chlorococcus dulciventrare ( Chlorococc), which is resistant to high concentration carbon dioxide.
um dorsiventrale ).
細藻類クロロコックム ドルシベントラレ クラノ エ
ト チハラ エスピー ノブ（Chlorococcum dorsiventr
ale Kurano et Chihara sp. nov. ）株。2. A novel microalga, Chlorococcum dorsivento, which is resistant to high concentration of carbon dioxide ( Chlorococcum dorsiventr).
ale Kurano et Chihara sp. nov.) strain.
細藻クロロコックムドルシベントラレ（Chlorococcum d
orsiventrale）を用いて排ガス中の二酸化炭素を固定す
る方法。3. A new microalga chloro cock arm to $ Bentora LES having a high concentration of carbon dioxide in the resistance (Chlorococcum d
orsiventrale ) to fix carbon dioxide in exhaust gas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20311893A JP3509902B2 (en) | 1993-08-17 | 1993-08-17 | New microalgae and method for fixing high concentration carbon dioxide using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20311893A JP3509902B2 (en) | 1993-08-17 | 1993-08-17 | New microalgae and method for fixing high concentration carbon dioxide using the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0751051A true JPH0751051A (en) | 1995-02-28 |
| JP3509902B2 JP3509902B2 (en) | 2004-03-22 |
Family
ID=16468705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20311893A Expired - Fee Related JP3509902B2 (en) | 1993-08-17 | 1993-08-17 | New microalgae and method for fixing high concentration carbon dioxide using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3509902B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007047805A2 (en) * | 2005-10-20 | 2007-04-26 | Saudi Arabian Oil Company | Carbon neutralization system (cns) for co2 sequestering |
| JP2013535957A (en) * | 2010-06-23 | 2013-09-19 | アー．ホータ．ストロイアッツォ−モウギン ベルナルド | Method for obtaining desired pharmaceutical and nutritional fatty acids |
| WO2020071444A1 (en) * | 2018-10-02 | 2020-04-09 | 国立研究開発法人科学技術振興機構 | Method for culturing fresh water microalga |
| JP2020536571A (en) * | 2017-10-11 | 2020-12-17 | ウニベルシダッド デ アントファガスタ | Species of the genus Muriellopsis, a microalga for producing biomass with high lutein content and low metal content, which has good antioxidant properties and is useful in the preparation of foods for animal or human consumption. ) Method for outdoor culture |
-
1993
- 1993-08-17 JP JP20311893A patent/JP3509902B2/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007047805A2 (en) * | 2005-10-20 | 2007-04-26 | Saudi Arabian Oil Company | Carbon neutralization system (cns) for co2 sequestering |
| WO2007047805A3 (en) * | 2005-10-20 | 2007-07-05 | Saudi Arabian Oil Co | Carbon neutralization system (cns) for co2 sequestering |
| JP2013535957A (en) * | 2010-06-23 | 2013-09-19 | アー．ホータ．ストロイアッツォ−モウギン ベルナルド | Method for obtaining desired pharmaceutical and nutritional fatty acids |
| JP2020536571A (en) * | 2017-10-11 | 2020-12-17 | ウニベルシダッド デ アントファガスタ | Species of the genus Muriellopsis, a microalga for producing biomass with high lutein content and low metal content, which has good antioxidant properties and is useful in the preparation of foods for animal or human consumption. ) Method for outdoor culture |
| WO2020071444A1 (en) * | 2018-10-02 | 2020-04-09 | 国立研究開発法人科学技術振興機構 | Method for culturing fresh water microalga |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3509902B2 (en) | 2004-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pringsheim | Pure cultures of algae | |
| Hertzberg et al. | Studies of alginate-immobilized marine microalgae | |
| CN105660357B (en) | A kind of artificial brackish water ecological breeding method of Enteromorpha | |
| CN103952315A (en) | Plankton strain algae Parachlorella nurekis 1904 KIEG and its use for biomass production and eradication of cyanobacteria, bacteria and fungi | |
| JP3509902B2 (en) | New microalgae and method for fixing high concentration carbon dioxide using the same | |
| Ailstock et al. | The characterization of axenic culture systems suitable for plant propagation and experimental studies of the submersed aquatic angiosperm Potamogeton pectinatus (sago pondweed) | |
| Takahashi et al. | Micropropagation of virus free bulblets of Lilium longiflorum by tank culture-1. Development of liquid culture method and large scale propagation | |
| JP2006129833A (en) | Method for culturing sargassum | |
| Turpin et al. | Limiting nutrients of oyster pond seawaters in the Marennes-Oléron region for Haslea ostrearia: applications to the mass production of the diatom in mesocosm experiments | |
| Kristiansen et al. | Salinity-temperature effects on growth and reproduction of Scytosiphon lomentaria (Fucophyceae) along the salinity gradient in Danish waters | |
| CN112841093B (en) | Biomembrane carrier and domestication method thereof | |
| CN1146290A (en) | Live single-cell sea red-yeast ecological bait and production method | |
| JP4411391B2 (en) | Algal growth method and algal culture obtained by the method | |
| CN115449485B (en) | Method for culturing marine chlorella | |
| JP2991512B2 (en) | New algae and carbon dioxide immobilization method using the same | |
| RU2793471C1 (en) | Method for cultivation of chaetoceros muelleri and isochrysis galbana unicellular microalgae - live food for larvae of marine invertebrates | |
| GINZBURG et al. | The adaptation of Dunaliella to widely-differing salt concentrations | |
| CN110055209B (en) | Preparation method of Talaromyces protoplast | |
| WO2020027002A1 (en) | Method for producing seaweed cells | |
| RU2675318C2 (en) | Method of growing microalga porphyridium purpureum | |
| KR100468045B1 (en) | Development of profitable prey, freshwater green algae, Synechocystis sp. | |
| GOTO | In vitro organogenesis from leaf explants of some dwarf mutants of Arabidopsis thaliana (L.) Heynh. | |
| JP2001190271A (en) | Euglena and method for cultivating the same using deep sea water | |
| JP2005312432A (en) | Immature unialgal culture stain, method for producing the same and proliferated alga body | |
| JPH09271388A (en) | New fine alga and production of highly unsaturated fatty acid using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20031224 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313117 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080109 Year of fee payment: 4 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090109 Year of fee payment: 5 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090109 Year of fee payment: 5 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313117 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090109 Year of fee payment: 5 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100109 Year of fee payment: 6 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110109 Year of fee payment: 7 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120109 Year of fee payment: 8 |
|
| LAPS | Cancellation because of no payment of annual fees |