JPH07294516A - Blood collecting tube - Google Patents

Abstract

PURPOSE:To provide a blood collecting tube enabling the separation operation of blood by a low centrifugal force and requiring no complicated equipment. CONSTITUTION:In the blood collecting tube consisting of a blood sampling tube main body 1 having a curved surface bottom opened at one end thereof and closed at the other end thereof and a serum separation agent 4 received in the blood collecting main body and having an intermediate specific gravity between serum to be separated or a plasma component and blood clot or a corpuscle component, the surface inclined from the inner peripheral surface 5 of the tube wall toward the bottom surface 6 of the blood collecting tube main body 1 is provided in at least the bottom part 2 receiving the serum separation agent 4 of the main body 1.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a blood collection tube for separating serum.

[0002]

2. Description of the Related Art Conventionally, blood collection tubes used for clinical tests are
For example, as shown in the sectional view in the tube axis direction in FIG. 1, a serum separating agent 14 is contained in the bottom portion 12 of the blood collection tube 11. Since this serum separating agent 14 has an intermediate specific gravity between the serum or plasma component and the blood clot or blood cell component, for example, when centrifugal force is applied, it moves to the middle of the serum or plasma component and the blood clot or blood cell component, and the separating agent layer Has the action of separating both components. However, since the bottom surface 16 of the blood collection tube 11 is spherical,
When the centrifugation operation was performed, there was a problem that the centrifugal force did not act effectively, the serum separating agent 14 did not sufficiently move, and inversion failure occurred.

The term "reversal failure" as used herein means that when a blood sample is placed in a blood collection tube containing a separating agent at the bottom and a separating operation is performed by a centrifuge, the separating agent is sufficiently moved. Since it does not exist, a separating agent layer is not formed between the serum or plasma layer and the blood clot or blood cell layer, and the serum or plasma layer and the blood clot or blood cell layer are not completely separated.

In order to prevent such reversal failure, methods such as applying a larger centrifugal force or prolonging the time of the centrifugal separation operation have been carried out, but this method complicates the separation work. There was a problem that

As a blood collection tube for preventing such reversal failure, (1) a blood collection tube in which spherical particles having different specific gravities are placed on a separating agent (JP-A-52-137181),
(2) A blood collection tube in which a dent is provided on the upper surface of the separating agent (Japanese Patent Laid-Open No. 60-65) is disclosed.

However, in the blood collection tube of the above (1),
There is a problem that the reliability of the test value is reduced because the test value is adversely affected by the destruction of blood cells, etc. In the blood collection tube of (2) above, the equipment is complicated due to the provision of the depression, and the economical efficiency is reduced. There was a problem of being damaged.

[0007]

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned drawbacks, and an object of the present invention is to enable production without requiring complicated equipment and to separate blood with low centrifugal force. An object is to provide an operable blood collection tube.

[0008]

The blood collection tube of the present invention comprises a bottomed blood collection tube main body and a separating agent contained in the blood collection tube main body.

Examples of the material of the blood collection tube include glass and plastic, and the plastic includes polyethylene terephthalate, nylon, polypropylene,
Polyethylene or the like is preferably used.

As shown in the longitudinal sectional view of FIG. 2, the main body of the blood collection tube has a shape in which one end is open and the other end is closed. A bottom surface 2 of the blood collection tube main body 1 is provided with a surface 3 that is inclined from the inner peripheral surface 5 of the tube wall toward the bottom surface 6.

The inclined surface 3 does not have to be provided from the entire inner peripheral surface 5 of the pipe wall toward the bottom surface 6 as shown in the enlarged perspective view of FIG. It may be formed in one place toward the bottom surface 6, and may be flat or curved. Further, when the surface 3 is provided as a columnar or complicatedly shaped ridge, bubbles may be entrained in the separating agent during the centrifugation operation, which is not preferable.

When the outer diameter of the blood collection tube main body 1 is 16 mm, the surface 3 is preferably formed to have a height of about 10 to 20 mm from the bottom surface 6. Such inclined surface 3 can be easily provided by integrally molding by injection molding or the like when the blood collection tube body 1 is manufactured.

In the blood collection tube of the present invention, the separating agent 4 is contained in the bottom portion 2 of the main body 1 of the blood collection tube. The separating agent 4 preferably has an intermediate specific gravity (1.040 to 1.060) between the serum or plasma component to be separated and the blood clot or blood cell component, and further has hydrophobicity and thixotropic properties, More preferably, it is substantially inactive.

[0014]

In the conventional blood collection tube, since the tube bottom has a substantially hemispherical shape, a uniform centrifugal force is applied to the separating agent and the blood collection tube bottom in the centrifugal separation operation, and the separating agent is pressed against the tube bottom. Is thought to be difficult to move. On the other hand, by providing the surface of the blood collection tube of the present invention that is inclined from the inner peripheral surface of the tube wall toward the tube bottom portion, a non-uniform centrifugal force is applied to the separating agent in the centrifugal separation operation. It is considered that the particles can be easily moved and can be separated with a low centrifugal force.

[0015]

【Example】

(Examples 1 to 3) 100 blood collection tubes in which a separating agent was dispensed into the blood collection tube main body (made of polyethylene terephthalate) shown in FIG. 3 were used, and a saline solution having a specific gravity of 1.
08), and set in a swing-rotor centrifuge and subjected to centrifugal separation by the predetermined centrifugal force shown in Table 1. Then, the separation performance of the blood collection tube was judged according to the following criteria, and the number of each Is shown in Table 1. ◯: The separating agent moved to form partition walls of the separating agent on the surface of the saline. X: The separating agent did not move.

(Examples 4 to 6) Centrifugation was carried out by the predetermined centrifugal force shown in Table 1 in the same manner as in Example 1 except that human fresh blood was used instead of saline, and then, The separation performance of the blood collection tubes was judged according to the following criteria, and the number of each is shown in Table 1. ◯: The separating agent migrates and the thickness is 5 between the serum phase and the blood clot phase.
A partition wall having a size of mm or more was formed. X: The separating agent did not move.

(Examples 7 to 9) In the same manner as in Example 1 except that a blood collection tube in which a separating agent was dispensed was used in the blood collection tube body (made of polyethylene terephthalate) shown in FIG.
After centrifugation was carried out with the predetermined centrifugal force shown in Table 1, the separation performance of the blood collection tube was judged according to the same criteria as in Example 1,
The results are shown in Table 1.

(Examples 10 to 12) [0018] After centrifugation was carried out by the predetermined centrifugal force shown in Table 1 in the same manner as in Example 7 except that human fresh blood was used instead of saline, The separation performance of the blood collection tube was judged according to the same criteria as in Example 4, and the results are shown in Table 1.

[0019]

[Table 1]

Comparative Examples 1 to 3 In the same manner as in Example 1 except that a blood collection tube in which a separating agent was dispensed was used in the blood collection tube body (made of polyethylene terephthalate) shown in FIG.
After centrifugation was carried out with the predetermined centrifugal force shown in Table 1, the separation performance of the blood collection tube was judged according to the same criteria as in Example 1,
The results are shown in Table 2.

(Comparative Examples 4 to 6) Centrifugation was carried out by the predetermined centrifugal force shown in Table 1 in the same manner as Comparative Example 1 except that human fresh blood was used instead of saline, and then, The separation performance of the blood collection tube was judged according to the same criteria as in Example 4, and the results are shown in Table 2.

[0022]

[Table 2]

A thixotropic gel-like separating agent obtained by kneading cyclopentadiene resin, dibenzylidene sorbitol, silica and phthalic acid (2-ethylhexyl) as a separating agent (“Escollect” manufactured by Sekisui Chemical Co., Ltd., Specific gravity 1.05, viscosity 150,000 cp (25 ° C.)] was used.

[0024]

The structure of the blood collection tube of the present invention is as described above, the blood separation operation can be performed with a low centrifugal force, and the conventional blood collection device can be manufactured without requiring complicated equipment. Further, since it is not necessary to add a foreign substance other than the separating agent to the blood collection tube of the present invention, the test value is not adversely affected.

[Brief description of drawings]

FIG. 1 is a longitudinal sectional view showing a conventional blood collection tube.

FIG. 2 is a vertical sectional view showing an embodiment of a blood collection tube of the present invention.

FIG. 3 is an enlarged perspective view showing an inclined surface formed on the blood collection tube of the present invention.

FIG. 4 is a longitudinal sectional view showing an example of a blood collection tube used in an example of the present invention.

FIG. 5 is a longitudinal sectional view showing an example of a blood collection tube used in another example of the present invention.

[Explanation of symbols]

 1, 11 Blood collection tube main body 2, 12 Bottom part 3 Sloping surface 4, 14 Separation agent 5 Pipe wall inner peripheral surface 6, 16 Bottom surface

Claims (1)

[Claims] 1. A curved bottomed blood collection tube main body having one end open and the other end closed, and an intermediate specific gravity between a blood serum or plasma component and a blood clot or blood cell component to be separated and contained in the blood collection main body. A blood collecting tube comprising a serum separating agent having: a bottom surface of the blood collecting tube containing the serum separating agent, wherein a surface inclined from the inner peripheral surface of the tube wall toward the bottom surface is projected. And blood collection tube.