JPH07280805A - Acarid detector - Google Patents

Abstract

PURPOSE:To detect presence of acarids conveniently by providing a first antibody carrying part and a positivity deciding part for carrying a second antibody which can be bonded specifically to a protein derived from an acarid. CONSTITUTION:An acarid specimen liquid is migrated through a porous basic material 101 toward a first antibody carrying part 103 by capillary function. When a protein derived from acarid is present in the specimen liquid, a reaction for bonding the protein to a first antibody proceeds in the specimen liquid migrating through the basic material. The acarid specimen liquid from an applied part 102 passes through a migration retardant part 104 and a second antibody arrives at a positivity deciding part 105 fixed to the basic material 101. A bonding reaction to the second antibody takes place to develop a specific color at the positivity deciding part 105 when a complex with the first antibody is present, otherwise the deciding part 105 is passed and the color is not develop clearly thus detecting presence of acarids.

Description

Detailed Description of the Invention

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus and method capable of easily detecting the presence or absence of mites.

[0002] mites inhabiting Background chamber is a cause of asthma and various allergies. It is considered that knowing the presence or absence of these mites and, if present, the type of the mites is useful for the prevention and treatment of asthma and various allergies. As a method for detecting mites, it is conceivable to directly observe dust in a room with a microscope, but it is difficult to say because it requires the observer to have specialized knowledge. Therefore, some methods have been proposed as simple methods. For example, a method in which indoor dust is treated with a chemical to develop a protein unique to mites and to detect its presence (Japanese Patent Publication No. 1-35).
No. 293, Japanese Patent Publication No. 3-58667, Japanese Patent Publication No. 4-645
91, etc.), an immunological assay using an enzyme-labeled antibody (JP-A-63-191961, JP-A 2-1).
61356). According to these methods and the apparatus for carrying out the method, it is possible to know the presence or absence of mites at a constant efficiency, but it is still desired to have a device that can more easily detect mites at home. I can say.

SUMMARY OF THE INVENTION Therefore, an object of the present invention is to provide a device capable of more easily detecting the presence or absence of mites.

Specifically, the first detection device according to the present invention is a device for detecting mites for determining the presence or absence of a mite-derived protein in a mite sample liquid, wherein the base material is a mite sample liquid. Is composed of a developable porous material, the substrate is a sample solution application site to which a mite sample solution is applied, a first antibody-supporting site carrying a first antibody capable of specifically binding to a mite-derived protein And a detection device having a positive determination site carrying a second antibody different from the first antibody capable of specifically binding to a mite-derived protein.

Further, as a preferred embodiment 1 of the first detection apparatus according to the present invention, a development delay site for delaying the deployment of the sample liquid is further provided between the site for supporting the first antibody and the site for positive determination. A detection device is provided.

Further, as a second preferred embodiment of the first detecting device according to the present invention, there is provided a detecting device further comprising a part where the sample liquid cannot be developed in a part of the base material near the positive determination part.

Further, as a preferred embodiment 3 of the first detection device according to the present invention, a third substance capable of specifically binding to the first antibody is further immobilized on the downstream side in the developing direction of the sample liquid from the positive determination site. A detection device is provided.

The second detection device according to the present invention is a device for detecting mites that determines the presence or absence of a mite-derived protein in a mite sample liquid, which is capable of containing a mite sample liquid. An opened container, a sealing lid having a sealing film attached to the one side opening of the container, and a sealed detection body attached to the sealing lid, the detection body being provided with a detection unit A cutting portion that breaks the seal film when the body is attached to the sealing lid,
There is provided a detection device including a determination unit that is provided continuously to the cutting unit and that determines the presence or absence of a mite-derived protein.

Further, as a preferred embodiment of the second detecting device, the judging section comprises a detecting plate for permeating a mite sample liquid and a detecting plate capable of specifically binding to a mite-derived protein linked to the detecting plate. A first antibody-carrying portion carrying one antibody, and a first antibody provided on this detection plate, different from the first antibody,
Provided is a detection device comprising a second antibody-immobilized part to which a second antibody capable of specifically binding to a mite-derived protein is immobilized, and an absorber for absorbing a mite sample solution that has passed through. .

A third detection device according to the present invention is a mite detection device for determining the presence or absence of a mite-derived protein in a mite sample liquid, which is capable of accommodating a mite sample liquid and is open on one side. A container and a detector attached to one opening of the container, the detector having a communication hole sealed with a water-soluble film, and a case attached to the one opening of the container, There is provided a detection device provided in a case and having a determination unit for determining the presence or absence of a mite-derived protein.

Further, as a preferred embodiment of the third detecting device, the judging section comprises a detecting plate for permeating a mite sample solution and a detecting plate capable of specifically binding to a mite-derived protein linked to the detecting plate. A first antibody-carrying portion carrying one antibody, and a first antibody provided on this detection plate, different from the first antibody,
Detection comprising a second antibody-immobilized part to which a second antibody capable of specifically binding to a mite-derived protein is immobilized, and an absorber for absorbing the mite sample solution and antibody-sensitized particles that have passed through A device is provided.

Further, as a fourth detection device according to the present invention, a mite detection device for determining the presence or absence of a mite-derived protein in a mite sample liquid, which is capable of containing the mite sample liquid, A container that is opened and is closed on the other side, a partition that opens and closes the inside of the container into a solution chamber on the opening side and a detection body chamber on the sealed side, and a sealing lid attached to the opening of the container Provided is a detection device including a determination unit for determining the presence or absence of a mite-derived protein in the detection body chamber.

[Detailed Description of the Invention] The tick detection device according to the present invention is based on an immunological method, which is a so-called sandwich method, which uses an antibody against a tick-derived protein. That is, the presence or absence of a mite-derived protein is determined by using a first antibody and a second antibody that can specifically bind to the protein, and thereby the presence or absence of mites is to be known. Therefore, in the present specification, the first antibody and the second antibody mean, for example, antibodies against different epitopes of a mite-derived protein.

The first antibody and the second antibody may be a monoclonal antibody or a polyclonal antibody produced by a well-known method that has already been established, but are preferably monoclonal antibodies because of their binding specificities. Further, the protein derived from a mite which is an antigen may be appropriately selected depending on the mite to be detected, but it is preferable that the antigen is a protein derived from a leopard mite, a mite, a mite, and a mite, which often inhabit the house.

Further, either the first antibody or the second antibody is labeled, and in the detection device described below, it is preferable that the first antibody is labeled. In the detection device according to the present invention, the signal from the label is preferably observable with the naked eye, and the label is preferably present on the first antibody. Specific examples of preferable labels include enzyme labels such as alkaline phosphatase, β-galactosidase and peroxidase; fluorescent labels such as fluorescein and rhodamine; dye labels such as red blood cells and colored latex; metals such as gold, silver and selenium, and Examples thereof include inorganic particles.

In the present invention, dust, dust or dirt which may contain mites are collected, a mite sample solution is prepared therefrom, and the solution is applied to the apparatus according to the present invention to determine the presence or absence of mites. Know the existence.

Dust, dust, and dust that may contain mites, collected by a vacuum cleaner, or obtained by passing it through a mesh (for example, 16 mesh) having an appropriate fineness, and further, indoors Prepare a sample collected by sticking an adhesive tape on the floor.

The dust, dust or dirt is put in water, physiological saline, a buffer solution such as a phosphate buffer solution, and stirred.
The solution thus obtained may be applied to the apparatus according to the present invention as it is, or may be further stirred to crush dust, dirt or dust to obtain a solution. That is, since the detection device according to the present invention detects a mite-derived protein as described above, it is desirable that the protein be dissolved in the solution by crushing the individual mite. Furthermore, a filler (for example, a glass filler, a metal filler, a ceramic filler, etc.) may be present at the same time so that the mites can be efficiently crushed, and the mixture may be agitated. Also, using a container with an uneven inner wall, The individual may be crushed and stirred.

The mite sample solution thus prepared is
It is applied to the tick detection device according to the present invention.

A preferred embodiment of the first detection device according to the present invention is shown in FIG. The detection device shown in the figure is based on the substrate 1.
01 in order from one end, a sample solution application site 102 to which a mite sample solution is applied, a first antibody carrying site 103, a sample development delay site 104, a positive determination site 105 and a negative determination site 1
06 is provided.

The substrate 101 of the detection device according to the present invention is made of a porous material capable of developing the above-mentioned mite sample liquid. Here, the spread of the mite sample solution means that the mite sample solution can move through the porous material of the substrate by the capillary action. Preferred examples of the base material include glass fiber, cellulose, and filter paper made of them, nylon non-woven fabric, polyester non-woven fabric, nitrocellulose, polyvinylidene fluoride, regenerated cellulose, cellulose acetate, a porous body of charge-introduced nylon, and the like. Particularly, those having a function of fixing proteins are preferable.

The sample application site 102 is a site to which the mite sample solution is applied. After the sample solution is applied, the base material 101 is applied.
The first antibody-carrying site 10 by the capillary action in the porous body of
The deployment movement is started toward 3.

The first antibody carrying portion 103 is a portion in which the first antibody is carried on the base material 101. This first antibody has the ability to specifically bind to a mite-derived protein. Therefore, when a mite-derived protein is present in the mite sample solution that has spread and moved from the sample application site 102, the reaction in which this protein and the first antibody bind to form a complex spreads and moves in the base material. It will proceed in the sample liquid.

After binding with the substance to be detected, the first antibody is
It is necessary to move as the mite sample liquid develops. Therefore, it is preferable that the substrate is impregnated with the first antibody, and details of a preferable supporting method will be described later.

The mite sample liquid that has spread and moved from the sample liquid application site 102 passes through the expansion delay site 104, which will be described later, and then reaches the positive determination site 105. Positive judgment part 1
Reference numeral 05 is a portion in which the second antibody is fixed to the base material 101.

When a complex of a mite-derived protein and the first antibody is present in the mite sample liquid, when the sample liquid reaches the positive determination site 105, a binding reaction between the complex and the second antibody occurs. By recognizing this binding using a labeling substance described later, the presence or absence of the test substance can be determined. That is, for example, when the first antibody is labeled with colloidal gold, the complex of the substance to be detected and the first antibody binds to the second antibody of the positive determination site 104 and remains at the positive determination site and accumulates. . As a result, the coloration of the gold colloid is observed at the positive determination part 104.
On the other hand, when the substance to be detected does not exist, the first antibody labeled with colloidal gold does not bind to the second antibody,
After passing through the positive-judgment site 105, no clear gold colloid coloration is obtained in the positive-judgment site 5. As described above, it is possible to know the presence or absence of a mite-derived protein in the mite sample liquid, that is, mite.

According to a preferred embodiment 101 of the first device of the present invention, the expansion delay portion 104 is provided in front of the positive determination portion 105. The development delay site 104 is a site that acts to delay the development of the mite sample solution, that is, to reduce the development rate of the sample solution. As a result of the decrease in the development speed, it takes more time for the mite sample solution that has come into contact with the first antibody at the first antibody-carrying site 103 to reach the positive determination site 105. by this,
The time required for the binding reaction between the mite-derived protein and the first antibody is increased, and the amount of the complex formed by binding the mite-derived protein and the first antibody is determined by the expansion delay site 1
It is larger than that when 04 does not exist.

According to a preferred embodiment of the present invention, the expansion retarding portion 104 is formed by, for example, supporting a water-soluble polymer on the base material, reducing the porosity of the base material, or a sample liquid on a part of the base material. It is a part formed by reducing the effective cross-sectional area of the base material on which the sample liquid can spread by providing the non-deployable part.

For example, in the case where a water-soluble polymer is supported on the substrate, the sample liquid that has developed and moved is absorbed by the polymer when it comes into contact with the water-soluble polymer. Then, the absorption continues until the amount of the sample liquid that has developed and moved exceeds the absorption capacity of the carried polymer. During this period, the sample liquid will continue to stay in this polymer, and it is expected that the reaction between the analyte and the first antibody will proceed sufficiently during the stay.

As the water-soluble polymer, those which do not affect the analyte, the first antibody and the second antibody, and their binding reaction are preferable. Specific examples thereof include polyacrylic acid, polyvinyl alcohol, polyvinylpyrrolidone. , Polyacrylamide, polydimethylacrylamide, polyethylene glycol, polypropylene glycol, polyvinyl methyl ether, casein, gelatin,
Examples thereof include hydroxypropyl cellulose.

The expansion retarding portion 104 is formed by reducing the porosity of the base material, for example, the base material 101.
A method of allowing particles or fillers smaller than the voids to be present in the porosity of. When the porosity of the base material becomes low as described above, it becomes resistant to the spread of the sample liquid and the spread speed is lowered. Due to the increased development time, it is expected that the reaction between the substance to be detected and the first antibody will proceed sufficiently. Preferable examples of the particles or fillers for reducing the porosity include polymer particles such as polystyrene and acryl, micro silica, alumina sol and the like.

Furthermore, according to a preferred embodiment of the present invention, the development delay portion 104 may be formed by providing a portion where the sample liquid cannot be developed on a part of the base material. A preferred specific example is shown in FIG. FIG. 2 is a cross-sectional view of the development delay portion 104 portion of the detection device according to the present invention, in which 104a is a base material 1 provided with a region where the mite sample solution cannot be developed. Due to the presence of the site where the sample liquid cannot be developed, the mite sample liquid has its development flow path restricted (arrow in the figure), and thereby the development speed is expected to decrease.

Such a sample liquid non-developable portion can be formed by making a part of the base material insoluble in water by, for example, an insoluble polymer. As the water-insoluble polymer, those which are substantially insoluble in water and which do not affect the analyte substance, the first antibody and the second antibody and their binding reaction are preferable, and specific examples thereof include polyacrylate, Polymethyl methacrylate, polyvinyl acrylate, polybutyl methacrylate, polystyrene, α-
Methyl polystyrene, chloromethylated polystyrene, polyvinyl acetate, polybutadiene, polyisoprene, polyacrylonitrile, polyvinyl butyral, polyacrylamide, polyvinyl carbazole, and other vinyl-based or polymers alone or copolymers composed of two or more kinds of respective monomers Examples thereof include polyamides, polyesters, polyurethanes and the like obtained by ring-closing polymerization or condensation polymerization, celluloses such as hydroxypropylmethyl cellulose phthalate, carboxymethyl ethyl cellulose and ethyl cellulose, shellac and the like.

Further, a polymer obtained by treating with ionizing radiation after mixing one or more mono- or more functional ionizing radiation functional monomers or oligomers in the molecule, or a combination of two or more kinds or the above-mentioned polymers is also preferably used. The compound having a functional group in the molecule is preferably a compound which basically reacts to ionizing radiation such as ultraviolet rays and electron beams, and particularly a compound having a radical-polymerizable unsaturated bond. Monofunctional monomers include acrylic acid, acrylic acid dimer, 2-hydroxyethyl acrylate, 2-hydroxypropyl acrylate, methyl acrylate, 2-ethylhexyl acrylate, methoxyethyl acrylate, butyl acrylate, methoxybutyl acrylate, phenyl acrylate, etc. And esters thereof; methacrylic acid, 2-hydroxyethyl methacrylate, 2-hydroxypropyl methacrylate, glycerol methacrylate, methyl methacrylate, ethyl methacrylate,
Methacrylic acid esters such as propylmethacrylate, methoxyethylmethacrylate, ethoxyethylmethacrylate, phenylmethacrylate, laurylmethacrylate; unsaturated carboxylic acid amides of acrylamide, propylacrylamide, propylmethacrylamide, methacrylamide; 2- (N, N-dimethylamino) ) Ethyl acrylate, 2- (N, N-dimethylamino) ethyl methacrylate, 2- (N, N-dibenzylamino)
Substituted amino alcohol esters of unsaturated acids such as ethyl acrylate, (N, N-dimethylamino) methyl methacrylate, 2- (N, N-diethylamino) propyl acrylate; N-methylcarbamoyloxyethyl acrylate, N-ethylcarba Carbamoyloxyalkyl acrylates such as moyloxyethyl acrylate, N-butylcarbamoyloxyethyl acrylate, N-phenylcarbamoyloxyethyl acrylate, 2- (N-methylcarbamoyloxy) ethyl acrylate, and 2-carbamoyloxypropyl acrylate. Others Vinylbenzyltrimethylammonium chloride, N-methyl-4-vinylpyridinium chloride, 2-methacryloyloxyethyltrimethylammonium chloride, 2-
Examples thereof include cationic vinyl monomers such as acroyloxydimethylsulfonium chloride and acryloylmorpholine.

The polyfunctional monomer has two or more radically polymerizable unsaturated groups, and a polyfunctional (meth) acrylate having two or more (meth) acryloyl groups is particularly preferable. Specific examples include, for example, ethylene glycol di (meth) acrylate, polyethylene glycol di (meth) acrylate, hexanediol di (meth) acrylate, trimethylolpropane tri (meth) acrylate, trimethylolpropane di (meth).
Acrylate, pentaerythritol tetra (meth) acrylate, pentaerythritol tri (meth) acrylate, dipentaerythritol hexa (meth) acrylate, ethylene glycol diglycidyl ether (meth) acrylate, polyethylene glycol diglycidyl ether di (meth) acrylate, propylene glycol Examples thereof include polyfunctional (meth) acrylates such as diglycidyl ether di (meth) acrylate, polypropylene glycol diglycidyl ether di (meth) acrylate, sorbitol tetraglycidyl ether tetra (meth) acrylate, and melamine (meth) acrylate.

Examples of the oligomer or polymer having a functional group include urethane acrylate obtained by combining diisocyanate, a diol having a hydroxyl group at both ends and an acrylate compound having a hydroxyl group at both ends and a radically polymerizable unsaturated group. Yes, a urethane acrylate having an arbitrary molecular weight can be obtained by changing the molecular weight of the diol used. In addition, a 1: 1 adduct of acrylic acid chloride or 2-hydroxyethyl acrylate and diisocyanate is added to the hydroxyl group of the copolymer of polyester acrylate, epoxy acrylate, acrylic acid alkyl ester and acrylic acid-2-hydroxy ester. Acryloyl group-introduced copolymer, a copolymer in which glycidyl rotacrylate is added to the carboxyl group of a copolymer of alkyl acrylate and acrylic acid, or acrylic acid chloride or acrylic acid on the residual hydroxyl group of polyvinyl butyral. Those obtained by adding a 1: 1 adduct of 2-hydroxyethyl acid and diisocyanate can also be preferably used.

Unlike the case of the first antibody, the second antibody is preferably bound and supported on the base material 101 with a certain strength. Details of preferable specific examples of the supporting method will be described later.

Preferred Embodiment 2 of the First Device of the Present Invention
According to the above, a part where the sample liquid cannot be developed is provided in a part of the base material near the positive determination part 105, so that all or part of the spreading and moving tick sample liquid converges in the vicinity of the positive determination part 105. Formed. The detection device shown in FIG. 1 includes a part 107a of the base material on both sides of the positive determination part 105a.
And 107b are water-insoluble examples. Due to the presence of the site where the sample liquid cannot be developed, the effective cross-sectional area of the base material is narrowed so that the sample liquid converges near the positive determination site 105. As a result, a larger amount of the sample liquid will pass in the vicinity of the positive determination region 105, as compared with the case of using a base material that does not have such a region in which the sample liquid cannot be developed. As a result, when the sample liquid contains a complex of the substance to be detected and the first antibody, more complexes will be involved in binding with the second antibody, which is advantageous.

Another preferred example of the site where the sample liquid cannot be developed will be described with reference to FIG. FIG. 3 is a cross-sectional view near the positive determination part 105 of the first detection device according to the present invention. Figure 3
The detection device shown in 1 is an example in which the sample liquid undevelopable portion is provided by making the positive determination portion 105 of the base material 1 water-insoluble at a certain thickness from the back surface. The non-water-solubilized portion causes the sample liquid to converge on the positive determination portion 105 (arrow in the figure).

The formation of the water-insolubilized region according to the second aspect of the present invention utilizes the water-insoluble polymer used for forming the expansion retarding site in the second aspect of the present invention. It can be carried out.

Furthermore, according to a preferred embodiment of the present invention,
A negative determination part 106 is provided. This negative judgment part 1
06 carries a third antibody capable of specifically binding to the first antibody. The development of the mite sample solution causes the first antibody to be developed and moved from the first antibody carrying portion 103. If the substance to be detected does not exist in the sample liquid, the first antibody not bound to the substance to be detected reaches the negative determination site 106 and binds to the third antibody. When the first antibody or the third antibody carries a label that generates a signal by this binding, the presence or absence of this binding can be recognized. That is, the negative determination part 106
Whether or not the development is completed and the substance to be detected is absent can be determined more surely by the presence or absence of the labeling signal from. Also,
Similarly, when the first antibody-carrying site 103 is predominantly present in a larger number than the substance to be detected, two signals from the positive determination site 105 and the negative determination site 106 similarly indicate that the development has ended. The presence of the substance to be detected can be determined more reliably.

Although an example in which the first device according to the present invention and the preferred embodiments 1 and 2 are provided together has been described above, a detection device having only one of the embodiments may be provided depending on the case. Any of these are included in the scope of the present invention.

FIG. 4 shows a specific example of the detection device having the two aspects of the present invention independently. The left column in the drawing is a top view of the detection device, and the right column is its partial cross-sectional view. FIG. 4 (a) is an example in which a development delaying site 104 in which a water-soluble polymer is carried on a substrate is provided between the first antibody carrying site 103 and a positive determination site 105, and FIG. 4 (b) is a part of the substrate 1. Development delay site 10 formed by rendering water insoluble
4 is provided, the same (c) is an example in which the base material 101 on the opposite side of the positive determination part 105 is made non-water-soluble, and the development of the sample liquid is converged on the positive determination part 105, the same (d) is An example in which the base material 101 on both sides of the positive determination portion 105 is made water-insoluble so that the spread of the sample liquid is converged on the positive determination portion 105. The same (e) is a combination of the same (c) and the same (d). Each example is shown. Note that (f) shows a conventional detection device.

The above-mentioned first antibody, second antibody, water-soluble polymer or water-insoluble polymer may be dissolved or dispersed in a suitable solvent and applied to and impregnated on the substrate 101 by pipetting. Further according to a preferred aspect of the present invention, they may be applied using printing techniques. For example,
The first antibody, the second antibody, the water-soluble polymer or the water-insoluble polymer is used as an appropriate solvent (for example, water, alcohols, esters, ethers, aldehydes, ketones, benzenes, paraffins, etc.) alone. Alternatively, the composition dissolved in a mixed solvent) is applied to the substrate 101 by a method such as gravure printing, screen printing, blade coating, slide coating,
To be supported on. When such a printing method is used, the first antibody-supporting site, the development delay site, the positive determination site, the negative determination site, and the water-insolubilized site are formed on the substrate 108 that is an assembly of the base materials 101 as shown in FIG. It is possible to efficiently manufacture the detection device according to the present invention so that the detection device is formed by forming the above, and then separating along the dotted line in the drawing to form the detection device.

Here, since the first antibody needs to move with the development of the sample liquid, when the first antibody is carried on the base material 101, the functional group existing in the first antibody or the base material is
For example, it may be blocked with bovine serum albumin, collagen, casein or the like, and the first antibody may be supported so as not to be strongly immobilized. On the other hand, unlike the above-mentioned first antibody, the second antibody at the positive determination site needs not to move with the development of the sample liquid. Therefore, it is preferable that the positive determination site is dried or heated after printing to immobilize the second antibody on the substrate.

Next, the second detection device according to the present invention will be described. A second detection device according to the present invention is shown in FIG. This detection device is made of a synthetic resin container 2 with one side open.
11 and a synthetic resin sealing lid 215 attached to the opening side of the container 211. The sealing lid 215 is attached to the container 211 by engaging an inner screw 215a provided on the inner peripheral surface with an outer screw 211a provided on the outer peripheral surface of the container 211.

Further, the sealing lid 215 has a sealing film 217 at the end opposite to the container 211, and an outer screw 215b is provided on the outer circumference of the sealing lid 215. Further, the end of the sealing lid 215 on the container 211 side is the container 21.
It contacts the flange 219 provided on the No. 1 to maintain the hermeticity.

Further, the detection body 220 is attached to the sealing lid 215. The detection body 220 has a case 221 provided with an internal screw 221a, and is attached to the sealing lid 215 by engaging the internal screw 221a with the external screw 215b of the sealing lid 215. Case 221
Is made of a transparent synthetic resin material, and has a cutting portion 223 and a filter portion 225 in that order from the sealing lid 211 side.
And a detection plate 2 having a second antibody-immobilized portion 230 on which a second antibody capable of specifically binding to a mite-derived protein is immobilized.
27 and an absorbent pad 232 are provided. Of these, the detection plate 227 having the second antibody fixing portion 230 and the absorption pad 232 constitute a determination portion.

Of these, as shown in FIG. 7, the cutting portion 223 is supported by the case 221 via the supporting portion 221a, and when the detection body 220 is attached to the sealing lid 215,
The seal film 217 is designed to be broken. The filter unit 225 removes impurities in the mite sample liquid that flows in from the container 211 side, and the device shown in FIG. 6 has three stages. However, the filter unit 22
The number 5 is not limited to three stages, and may be less than this or more than this. Further, between the detection plate 227 and the filter unit 225, the first antibody-carrying unit 2 carrying the first antibody capable of specifically binding to the mite-derived protein is supported.
29 are provided.

The detection plate 227 is arranged along the longitudinal direction of the case 221 between the filter portion 225 and the absorption pad 232, and allows the mite sample liquid to permeate therethrough and to pass the first antibody that passes with the mite sample liquid. When positive, the antibody fixing unit 30 traps. The detection plate 227 is made of nitrocellulose, glass fiber filter, chromatography paper, PVDF (trade name), nylon-based or polyester-based nonwoven fabric, or the like. In addition, excess stool suspension and antibody-sensitized particles
It passes through the detection plate 227 and is absorbed by the absorption pad 232.

Next, detection of mites using the second detection device having such a configuration will be described.

First, the sealing lid 215 is removed from the container 211. Next, the mite sample solution is put into this container 211.
Further, without preparing a mite sample solution in advance as described above, put dust, dust or dirt that may contain mites in this container 211, and further add a solution such as water, physiological saline or a buffer solution. Alternatively, the mite sample solution may be prepared in this container. At this time, it is also preferable that the above-mentioned filler is further put in this container, or that the inner wall of this container 211 is provided with irregularities and is stirred with a stick or the like so that the mites are crushed more efficiently.

Next, the container 211 is sealed with the sealing lid 215. Container 211 sealed by a sealing lid 215
The ticks may be further crushed by shaking the.

After that, the case 221 of the detector 220 is attached to the sealing lid 215. That is, the outer screw 215b of the sealed body 215 is engaged with the inner screw 221a of the case 221, and the case 221 of the detection body 220 is provided outside the sealed body 215.
Push in while rotating. When the case 221 is completely attached to the closed lid 215, the cutting portion 223 breaks the seal film 217, and the mite sample liquid in the container 211 flows into the detector 220 side through the seal film 217. The mite sample liquid that has flowed into the detector 220 then passes through the filter unit 225. During this time, impurities in the mite sample liquid are removed by the filter unit 225.

Next, the mite sample liquid that has passed through the filter section 225 is absorbed by the absorption pad 2 inside the detection plate 227 together with the first antibody.
It penetrates toward the 32 side. Here, if a mite-derived protein is present in the mite sample solution, the reaction in which the protein and the first antibody bind to form a complex proceeds in the sample solution that spreads and moves on the detection plate 227. Then, the sample liquid reaches the second antibody fixing section 230, and when the complex is present in the sample liquid, a binding reaction between the complex and the second antibody occurs here. By recognizing this binding using the labeling substance preferably present in the first antibody, the presence or absence of a mite-derived protein, that is, the presence or absence of mites can be known.

Since the excess mite sample solution is absorbed by the absorbent pad 232, the mite sample solution can smoothly permeate into the detection plate 227. Next, referring to FIG. 9 and FIG.
Another embodiment of the second detection device according to the present invention will be described. Another embodiment shown in FIGS. 9 and 10 is
The only difference is the configuration of the detection plate and the absorption pad disposed inside the detection body, and the other points are substantially the same as those of the embodiment shown in FIGS.

That is, as shown in FIGS. 9 and 10, a filter portion 225 is provided in the case 221, and a developing spacer 232a is provided adjacent to the filter portion 225. Case 221 is container 2
11 (FIG. 1) is enlarged at the opposite end, and an annular pad chamber 233 is formed at this enlarged end. An absorption pad 232b is arranged in the pad chamber 233, and a detection plate 227 having a second antibody fixing portion 230 is arranged between the developing spacer 232a and the pad 232b so as to be orthogonal to the longitudinal direction of the case 221. There is.

A first antibody carrying portion 229 is provided between the filter portion 225 and the developing spacer 232a.

According to the present embodiment, the impurities in the mite sample liquid are more reliably removed by the filter portion 225 and the developing spacer 232a, and the mite sample liquid is detected by the detection plate 22.
It can be sent to the 7 side. The complex of the first antibody and the mite-derived protein trapped in the second antibody fixing section 230 can be easily confirmed from the bottom side (right side of FIG. 9) of the detector 220.

According to a preferred embodiment of the present invention, the absorbent pad 232 of the detection device may be formed by using a water-absorbent gel.

For example, as shown in FIG.
The absorbent pad 232 is made of the non-woven fabric 241 and the water-absorbing gel
42 may be dispersed and attached to the detection plate 227.

In this case, the nonwoven fabric is Condon REO.
-65 or Benliese RE750 (all manufactured by Asahi Kasei Corporation) can be used.

As the water-absorbent gel, commercially available products can be used. For example, Sumika gel (PVA / polyacrylate copolymer manufactured by Sumitomo Chemical Co., Ltd.), Diawet (Mitsubishi Petrochemical Co., Ltd., crosslinked) Polyacrylate-based), Aqua Reserve (Nippon Gosei Kagaku Co., Ltd., PV
A-type copolymer), Aqua Coke (Sumitomo Seika Co., Ltd.,
Cross-linked polyethylene oxide type), water-absorbing baogen (Daiichi Kogyo Seiyaku Co., Ltd., cross-linked polypropylene oxide type), aquaprene (Meisei Chemical Industry Co., Ltd., nonion type), sun wet (Sanyo Chemical Co., Ltd., starch / acrylic acid) Graft copolymer), KI gel (Kuraray Co., Ltd., isobutylene / maleic anhydride copolymer),
ALASORP (Arakawa Chemical Co., Ltd., polyacrylate type), Aquaric (Nippon Catalysis Chemical Co., Ltd., crosslinked polyacrylate type), Alonzap (Toa Gosei Co., Ltd.), Wonder Gel (Kao Corporation) , Crosslinked polyacrylate type) and the like.

As shown in FIG. 11 (b), the absorbent pad 232 has a water absorbent gel 2 inside a bag 243 made of non-woven fabric.
It may be configured to accommodate 42 and be attached to the detection plate 227. In this case, as the non-woven fabric and the water-absorbent gel, those similar to the absorbent pad shown in FIG. 11 (a) can be used.

Further, as shown in FIG. 11C, first, the water-absorbent gel 242, the binder, and the solvent are mixed to prepare a composition containing the water-absorbent gel 242, and the composition is placed on the detection plate 227. The absorbent pad 232 may be formed by applying and drying. In this case as well, the water-absorbent gel 242
As the absorbent pad, the same absorbent pad as shown in FIG. 11 (a) can be used.

Further, as shown in FIG. 11D, first, the water-absorbent gel 242 and a solvent (for example, alcohol + water) are mixed to prepare a water-absorbent gel solution, and the water-absorbent gel is placed on the polyethylene terephthalate substrate 245. Apply gel solution and dry. Next, the sheet-shaped absorbent pad 232 may be formed by irradiating this with an electron beam and crosslinking the water-absorbent gel. The sheet-shaped absorbent pad 232 is
The water-absorbent gel 242 side is brought into contact with the detection plate 227 side and attached to the detection plate 227. Also in this case, as the water absorbent gel 242, the same one as the water absorbent pad shown in FIG. 11A can be used.

An embodiment of the third detection device according to the present invention is shown in FIGS. As shown in FIGS. 12 and 13, the third detection device according to the present invention includes a cylindrical synthetic resin container 311 whose one side is open. This container 3
11, an inner screw 311a is provided on the inner surface on the opening side, and an outer screw 311b is provided on the outer surface on the opening side.

A sealing lid 313 having an inner screw 313a on the inner surface is attached to the opening side of the container 311 by engaging the inner screw 313a with the outer screw 311b of the container 311.

When the container 311 is used, the lid 31 is closed.
3, the detector 3 is attached to the container 311 instead. Next, the detection body 320 will be described in detail.

The detector 320 has a cylindrical case 321 provided with an external screw 321a. By engaging the external screw 321a with the internal screw 311b of the container 311,
The case 321 is attached to the container 311. Case 321
Is made of a transparent synthetic resin material, and a communication hole 322 sealed with a water-soluble film 323 is formed on the container 311 side of the case 321.

A communication hole 322 is provided in the case 321.
A filter portion 325 and a developing spacer 332a are arranged in order from the side. Further, the case 321 is enlarged at the end opposite to the communication hole 322, and an annular pad chamber 333 is formed at the enlarged end. Pad room 3
An absorption pad 332b is arranged in the casing 33, and a detection body 327 to which the second antibody fixing portion 330 is attached is provided between the developing spacer 332a and the absorption pad 332b.
It is arranged orthogonal to the axial direction (longitudinal direction) of 21.
Of these, the detection body 3 to which the second antibody fixing portion 330 is attached
27 and the absorbent pad 332b form a determination unit.

Further, the filter 325 and the developing spacer 3
A first antibody carrying part 329 carrying a first antibody is provided between the first and second antibody 32a.

Next, each component will be described. The filter part 325 is for removing impurities in the mite sample liquid that flows in from the container 311 side, and is configured in three stages in FIG. 12, but the filter part 325 is not limited to three stages and is less than this. Or it may be many. Further, the detection plate 327 includes a developing spacer 332a and an absorbing pad 33.
2b is arranged orthogonal to the longitudinal direction of the case 321 and allows the mite sample solution flowing in from the container 311 side to permeate through the second antibody fixing part when the first antibody passing with the mite sample solution is positive. It is designed to trap at 30. The detection plate 327 may be made of the same material as the detection plate 227 of the second detection device according to the present invention. The excess mite sample liquid passes through the detection plate 327 and is absorbed by the absorption pad 332b.

Next, detection of mites using the third detection device having such a configuration will be described.

First, the sealing lid 313 is removed from the container 310. Next, the mite sample liquid is put into this container 310. Further, as in the case of the second detection device according to the present invention, the mite sample liquid may be prepared in this container 310. Next, the case 321 is attached. Then, after a while, the water-soluble film 323 provided in the communication hole 322 of the case 321 is dissolved by the mite sample liquid, and the container 310
The mite sample liquid inside flows into the case 321 side through the communication hole 322. The mite sample liquid that has flowed into the case 321 is
After that, it passes through the filter unit 325. At this time, impurities in the mite sample liquid are removed by the filter unit 325.

Next, the mite sample liquid that has passed through the filter section 325 passes through the developing spacer 332a together with the first antibody carried by the first antibody carrying site 329, and passes through the detection plate 32.
The inside of 7 penetrates toward the absorbent pad 332b side. Here, if a mite-derived protein is present in the mite sample liquid, the reaction in which the protein and the first antibody bind to each other to form a complex proceeds in the sample liquid spreading and moving on the detection plate 327. Then, the sample liquid reaches the second antibody immobilization section 330, and when the complex is present in the sample liquid, a binding reaction between the complex and the second antibody occurs here. This bond
It is possible to know the presence or absence of a mite-derived protein, that is, the presence or absence of mites by recognizing by utilizing the labeling substance preferably present in the first antibody.

The excess mite sample liquid that has penetrated the detection plate 327 is absorbed by the absorption pad 332b, so that the mite sample liquid can smoothly penetrate into the detection plate 327.

Next, another embodiment of the third detecting device according to the present invention will be described with reference to FIGS. 14 and 15. The other examples shown in FIGS. 14 and 15 are different only in the configuration of the detection body, and the other points are substantially the same as the examples shown in FIGS. 12 and 13.

As shown in FIGS. 14 and 15, the detector 320 has a cylindrical case 321.
1 is a communication hole 322 sealed with a water-soluble film 323.
Is provided. In addition, the communication hole 3 is provided in the case 321.
The filter unit 325 and the second antibody fixing unit 33 in order from the 22 side
Detection plate 327 and absorption pad 332 to which 0 is attached
Is provided. Further, the filter unit 325 and the detection plate 3
A first antibody carrying portion 329 is provided between the case 27 and the detection plate 327, and the detection plate 327 is arranged along the axial direction of the case 321.

According to the present embodiment, when the mite-derived protein is present in the mite sample solution, the second antibody-immobilizing unit 330 is used.
A complex consisting of the first antibody and this protein is trapped in and shows a color. In this case, since the detection plate 327 to which the antibody fixing section 330 is attached is arranged along the axial direction of the case 321, it is possible to easily and clearly change the color of the first antibody fixing section 330 from the side of the case 321. I can confirm.

Even in the third detecting device, the absorbing pad 332 may be made of a water-absorbent gel as in the absorbing pad of the second detecting device according to the present invention.

The fourth detector according to the present invention will be described. A preferred embodiment of the fourth device according to the invention is shown in FIG.
6 to 19 show.

16 to 19, the mite detecting device includes transparent containers 411a and 411b made of synthetic resin or glass that can be split in two. Containers 411a, 4
11b are rotatable with respect to each other, and each container 41
1a and 411b are partition walls 413a and 413 which slide with each other.
b. Furthermore, one side of the container 411a is open.

Further, as shown in FIG. 19, the partition wall 413a of the container 411a and the partition wall 413b of the container 411b have communication holes 431a and 431b, respectively.
When a is rotated to a predetermined position with respect to the container 411b, the positions of the communication hole 431a and the communication hole 431b coincide with each other so that the inside of the container 411a and the inside of the container 411b communicate with each other.

When the containers 411a and 411b are combined,
Combination container 4 with one side open and the other side sealed
11a and 411b are configured, and this combination container 411
a and 411b are partition walls 413a and 413b that slide with each other.
The partition wall 413 made of a solution chamber 41 in the container 411a.
4 and the detection body chamber 415 in the container 411b. The solution chamber 414 in the container 411a can store the mite sample liquid. Also, the container 411b
Inside the filter section 418, in order from the partition wall 413 side,
Detection plate 417 (determination unit) having the second antibody fixing unit 430
And an absorption pad 420 are provided.

Of these, the filter section 418 is composed of a millipore filter, nittle cellulose, nylon mesh, Teflon mesh, glass filter, filter paper, PVDF, etc., and has not only one layer but also a multi-layer structure as required. In this case, the filtration efficiency can be improved by making the pore size of the downstream layer smaller than that of the upstream layer. Further, it is possible to improve the filtration efficiency by interposing a powder such as silica or alumina between the upstream side layer and the downstream side layer.

Further, a first antibody carrying section 419 carrying a first antibody capable of specifically binding to a mite-derived protein is provided between the filter section 418 and the detection plate 417. The first antibody carrying section 419 may be provided inside the filter section 418 or on the detection liquid 417.

The detection plate 417 is arranged between the filter portion 418 and the absorption pad 420 along the axial direction of the container 411b so as to allow the mite sample solution to permeate. The detection plate 417 is made of a hydrophobic porous film material such as nitrocellulose, glass fiber filter, chromatography paper, PVDF (trade name), nylon-based or polyester-based nonwoven fabric.

The excess mite sample liquid passes through the detection plate 417 and is absorbed by the absorption pad 420.
As the absorbent pad 420, cellulose, rayon, polyethylene, polypropylene microporous plastic, glass microfiber, Sumika gel or the like can be used.

Next, the detection of mites using the fourth detection device having the above structure will be described.

First, the mite suspension is placed in the solution chamber 414. Further, as in the case of the second detection device according to the present invention, the mite sample solution may be prepared in this solution chamber 414.

Next, the container 411a is rotated with respect to the container 411b, and the position of the communication hole 431a formed in the partition 413a of the container 411a is adjusted to the partition 413b of the container 411b.
The solution chamber 414 in the container 411a and the detection body chamber 415 in the container 411b are communicated with each other at the position of the communication hole 431b formed in the container 411a. Next, containers 411a and 411b
16 is placed vertically with the container 411a facing upward as shown in FIG.

In this case, the mite sample liquid in the solution chamber 414 is transferred to the communication holes 431a and 431 of the partition walls 413a and 413b.
It flows into the detection body chamber 415 through b. Detection body chamber 41
The mite sample liquid that has flowed into 5 is then filtered by the filter unit 418.
And the impurities in the mite sample solution are removed by the filter unit 418 during this time.

Next, the mite sample solution that has passed through the filter section 418 passes through the first antibody carrying section 419 and permeates the detection plate 417 toward the absorption pad 420 side. Here, when a mite-derived protein is present in the mite sample solution, the reaction in which the protein and the first antibody are bound to form a complex proceeds in the sample solution spreading and moving on the sample plate 417. Then, the sample liquid is placed in the second antibody fixing section 430,
When the complex is present in the sample liquid, a binding reaction between the complex and the second antibody occurs here. By recognizing this binding using the labeling substance preferably present in the first antibody, the presence or absence of a mite-derived protein, that is, the presence or absence of mites can be known.

Since the excess mite sample solution is absorbed by the absorption pad 420, the mite sample solution can smoothly permeate into the detection plate 417.

In the above-mentioned embodiment, the containers 411a and 411 are connected after the solution chamber 414 and the detector chamber 415 are communicated with each other.
Although the example in which b is left stationary in the vertical direction has been described, FIG.
As shown in, the containers 411a and 411b may be left stationary in the horizontal direction. In addition, although an example in which the containers 411a and 411b are rotated to connect the partition walls 413a and 413b to each other has been described, the partition wall 413 is not limited to this, and the partition wall 413 is formed of a film or the like, and a stick is attached to the sealing lid 421, whereby 413 may be configured to be broken.

Next, another embodiment of the fourth detector according to the present invention will be described with reference to FIGS. Figure 2
0 and FIG. 21 are different only in the configurations of the detection plate 417 provided with the second antibody immobilization unit 30 and the first antibody carrying unit 419, and the other embodiments shown in FIGS. It is almost the same as. Note that FIG. 20 and FIG.
1, the same parts as those in the embodiment shown in FIGS. 16 to 18 are designated by the same reference numerals and detailed description thereof will be omitted.

As shown in FIG. 20, a filter section 418 in the detection body chamber 415 is provided with a first antibody carrying section 419,
The mite sample solution passes through the first antibody carrying section 419. A detection plate 417 is provided below the first antibody carrier 419 at a predetermined interval. The detection plate 417 is arranged in the direction orthogonal to the axial direction of the container 411b, and the second antibody fixing portion 430 is attached to the detection plate 417 at substantially the center thereof.

Next, the detection of mites using this embodiment will be described. First, the mite sample liquid is put into the container 411a, and the sealing lid 421 is attached to the opening.

Next, the partition walls 413a and 413b are aligned so that the solution chamber 414 and the detection body chamber 415 communicate with each other, and the containers 411a and 411b are left standing vertically with the container 411a facing upward. Then, the mite sample solution in the solution chamber 414 flows into the detection body chamber 415, passes through the filter unit 418, and passes through the first antibody holding unit 419. After that, the mite sample liquid drops downward from the central portion of the first antibody carrying portion 419 drop by drop, abuts on the second antibody fixing portion 430 attached to the detection plate 417, and penetrates inside.

When a mite-derived protein is present in the mite sample solution, its presence can be confirmed in the second antibody immobilization section 430 by the same reaction as described above.

Even in the fourth detecting device, the absorbent pad 420 may be made of a water-absorbing gel like the second detecting device according to the present invention.

Further, according to a preferable aspect of the present invention, it is preferable to use a biodegradable material as a material constituting any of the above detection devices. Examples of such biodegradable materials include bionol (Showa High Polymer Co., Ltd., aliphatic polyester), Biopol (ICI, microbial-produced polyester), Mataby (Novamont, polyvinyl alcohol / starch type). And so on.

Example 1 Example 1 Rectangular (5 × 50 mm) Glass Fiber Paper (GF / DV)
A, manufactured by Whatman Co., Ltd.) is provided with an absorber made of cellulose pulp on the short side portion, and 20 mm from this absorber
1 μl of the anti-mite polyclonal antibody solution was dripped in a circular pattern using a dispenser at the location. Then, it was dried at 50 ° C. for 2 minutes to obtain a testing tool. A solution was prepared by dissolving 10 μg of mite antigen and 10 μl of anti-mite monoclonal solution labeled with gold colloid in physiological saline. The short side of the inspection tool opposite to the side where the absorber was provided was dipped in the solution. As a result, the round pattern portion on which the anti-mite polyclonal antibody solution was dropped was colored pink.

Example 2 A detector was manufactured as described in FIG. Detection plate 232
Was sprayed with an anti-mite monoclonal solution labeled with colloidal gold onto a detection plate having the second antibody binding part 230 manufactured in substantially the same manner as in Example 1, and dried at 50 ° C. for 2 minutes. An appropriate amount of dust collected by vacuuming an indoor carpet was put in a container 211 of this detection device, water was further added and the mixture was stirred, and then the detection body 220 was attached. As a result, the second antibody binding part 230 was colored pink. When this dust was inspected with a commercially available mite detection kit (dust checker, manufactured by Shinto Paint Co., Ltd.), the presence of mites could be similarly confirmed.

[Brief description of drawings]

FIG. 1 is a diagram showing a preferred example of a first detection device according to the present invention.

FIG. 2 is a preferred embodiment of the first detection device according to the present invention, showing that a part of the base material is made water-insoluble so that the development rate of the mite sample liquid is reduced. Fig.

FIG. 3 is a preferred embodiment of the first detection device according to the present invention, showing that a part of the base material is made water-insoluble so that the spread of the mite sample solution converges on the positive determination site. Fig.

FIG. 4 is a diagram showing a preferred example of the first detection device according to the present invention.

FIG. 5 is a diagram showing a preferred manufacturing example of the first detection device according to the present invention.

FIG. 6 is a side sectional view showing a preferred example of the second detection device according to the present invention.

7 is a sectional view taken along the line II-II of the detection device in FIG.

8 is a sectional view taken along line III-III of the detection device of FIG.

FIG. 9 is a partial side sectional view showing still another preferable example of the second detection device according to the present invention.

10 is a cross-sectional view taken along line VV of the detection device of FIG.

FIG. 11 is a side sectional view showing details of an absorbent pad formed by using an absorbent gel.

FIG. 12 is a side sectional view showing a preferred example of a third detection device according to the present invention.

13 is a cross-sectional view taken along the line II-II of the detection device of FIG.

FIG. 14 is a partial side sectional view showing still another preferable example of the third detection device according to the present invention.

15 is a cross-sectional view taken along the line IV-IV of the detection device of FIG.

FIG. 16 is a side sectional view showing a preferred example of a fourth detection device according to the present invention, in which the device is left stationary in a vertical direction.

17 is a perspective view of the detection device of FIG.

FIG. 18 is a side sectional view in which the detection device of FIG. 16 is left stationary in the horizontal direction.

19 is a plan view showing a partition wall 413 of the apparatus of FIG.

FIG. 20 is a side sectional view showing still another preferable example of the fourth detection device according to the present invention.

21 is a lower partial perspective view of the detection device in FIG. 20. FIG.

Claims (7)

[Claims] 1. A mite detection device for determining the presence or absence of a mite-derived protein in a mite sample solution, wherein the substrate comprises a porous material capable of developing a mite sample solution, wherein the substrate Is the sample fluid application site to which the mite sample fluid is applied,
A first antibody carrying site carrying a first antibody capable of specifically binding to a mite-derived protein and a second antibody different from the first antibody capable of specifically binding to a mite-derived protein were carried A detection device having a positive determination part. 2. A tick detection device for determining the presence or absence of a mite-derived protein in a mite sample solution, comprising: a container having one side open, capable of accommodating a mite sample solution; A sealing lid that is attached to the side opening and has a seal film, and a sealed detection body that is attached to the sealing lid, and the detection body is provided inside the sealing lid, and when the detection body is attached to the sealing lid. A detection device comprising: a cutting portion that breaks the seal film, and a determination portion that is provided continuously to the cutting portion and that determines the presence or absence of a mite-derived protein. 3. The detection unit, a detection plate for permeating a mite sample solution, and a first antibody-supporting unit for supporting a first antibody capable of specifically binding to a mite-derived protein linked to the detection plate, Different from the first antibody provided on this detection plate,
The detection device according to claim 2, comprising a second antibody-immobilized portion on which a second antibody capable of specifically binding to a mite-derived protein is immobilized, and an absorber for absorbing the mite sample liquid that has passed through. 4. A tick detection device for determining the presence / absence of a mite-derived protein in a mite sample liquid, comprising a container having one open side capable of containing a mite sample liquid, and one side of this container. With a detection body mounted in the opening, the detection body has a communication hole sealed with a water-soluble film, a case mounted in one side opening of the container, and derived from a mite provided in the case A detection device comprising: a determination unit that determines the presence or absence of protein. 5. The detection part, a detection plate for permeating a mite sample liquid, and a first antibody-supporting part for supporting a first antibody capable of specifically binding to a mite-derived protein linked to the detection plate, Different from the first antibody provided on this detection plate,
A second antibody-immobilized portion having a second antibody that can specifically bind to a mite-derived protein is immobilized, and an absorber for absorbing a mite sample solution and antibody-sensitized particles that have passed through. The detection device according to claim 4. 6. The detection device according to claim 5, wherein a filter portion for removing impurities in the mite sample liquid is provided between the communication hole in the case and the determination portion. 7. A mite detecting device for determining the presence or absence of a mite-derived protein in a mite sample liquid, comprising a container capable of containing a mite sample liquid and having one side opened and the other side sealed. A partition wall that opens and closes the inside of the container into a solution chamber on the opening side and a detection body chamber on the closed side, and a sealing lid attached to the opening of the container, and a mite-derived protein in the detection body chamber A detection device comprising a determination unit for determining the presence or absence of