JPH07241192A - Liposome containing magnetic bacterium magnetosome and gene and method for introducing gene into bacterium by utilization of the same - Google Patents

Abstract

PURPOSE:To simply introduce a desired gene into a cell in a short time by applying a magnetic field to a liposome containing the magnetron of a magnetic bacterium and the gene to introduce the liposome into the bacterium. CONSTITUTION:A liposome containing the magnetosome of a magnetic bacterium and a desired gene is prepared by a method comprising e.g. adding the magnetosome of the magnetic bacterium and the desired gene to a liposome suspension and subsequently applying a magnetic field to the prepared liposome to introduce the liposome into the cell.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a liposome useful for gene transfer into cells and a gene transfer method using the liposome.

[0002]

2. Description of the Related Art Introduction of genes into cells is useful for studying the functions and expression mechanisms of human and other animal genes, gene therapy studies and gene therapy. Conventionally, various methods for introducing such genes have been proposed, but among them, the method utilizing liposomes has been drawing attention as an excellent method. As a typical example thereof, JP-A Nos. 2-135092 and 4-108391 disclose that a liposome composed of a cationic lipid is electrostatically attached to a cell membrane that is negatively charged, and the gene of the gene is transferred through the cell membrane. Introduction into cells is disclosed.

[0003]

However, in the above method utilizing electrostatic attraction, it is difficult to introduce genes into cells indiscriminately and it is difficult to selectively and efficiently introduce the genes into target cells. there were. There is also a drawback that the introduction process requires a long time. Therefore, an object of the present invention is to provide a means capable of introducing a desired gene into a cell with high efficiency and in a short time and, if necessary, selectively introducing the gene into a target cell. It is in.

[0004]

[Means for Solving the Problems] Liposomes, that is, according to the present invention, there is provided a liposome containing a magnetosome of a magnetic bacterium and a gene as a solution to the above problems. The magnetosome used in the present invention is a magnetic bacterium having a size of about 500 to 100
It is a fine magnetite particle of 1500Å. Magnetosomes obtained from the same kind of bacteria are very uniform in size and shape, and it is difficult to artificially synthesize the same. A magnetic bacterium that produces such a magnetosome in the cells can be easily separated from fresh water or seawater by the method described in, for example, JP-A-62-61599. The magnetosome is usually covered with an organic film, but it may be used as it is in the present invention, or may be used in a state where the organic film is removed.

The gene introduced into the liposome is not particularly limited, and any gene used for transformation of microorganisms, animals, plants, etc. is applicable. The form of the gene is not limited at all, and examples thereof include plasmids, DNA fragments, R
NA fragments and the like. The liposome of the present invention is prepared by
For example, it can be carried out by adding required magnetosomes and genes to a lipid suspension forming a liposome and vortexing. Alternatively, it may be prepared by adding a magnetosome and a gene to a commercially available liposome suspension. The lipid used to prepare the liposome is not particularly limited.

Gene Transfer Method Further , according to the present invention, by applying a magnetic field to the liposome containing the above-mentioned magnetosomes and genes,
Provided is a method for introducing a gene into a cell, which comprises the step of inducing the liposome into the cell and bringing the cell into contact with the cell. The kind of magnetic field, the method of applying the magnetic field, etc. are not particularly limited as long as the magnetic field strength and the application of the magnetic field do not cause cell damage via the magnetosomes. In vivo and in vitro of the present invention
It can be used in any of. For example, in
As for in vitro use, when studying transient gene expression in animal cells, by using the method of the present invention, a gene can be introduced into a target cell simply and in a short time with high efficiency. be able to.

In vivo use, for example, the magnetosome-containing liposome of the present invention containing a gene for gene therapy of coronary artery restenosis is induced by a magnetic field into smooth muscle cells involved in coronary artery restenosis. It is considered that the gene is introduced into the cell by contacting with. It is also useful for transcatheter gene therapy in various other diseases.

[0008]

【Example】

Example 1 (1) A liposome containing a magnetosome isolated from a magnetic bacterium AMB-1 (Microtechnology Research Institute No. 13282) and a luciferase gene, which is a bioissue gene, was prepared as follows. Magnetosome and liposome were mixed at a weight ratio of 1/5 to 5/1 in sterilized distilled water and left standing for 15 minutes. Then, the required amount of gene was added to the mixed solution, mixed, and allowed to stand for 15 minutes.

Separately, smooth muscle cells (separated from rabbit aorta and cultured) were cultured in a plastic culture container in Dulbecco's modified Eagle medium containing 5% fetal bovine serum. The liposome containing the magnetosome and the luferase gene was administered to the smooth muscle cells thus cultured, and left to react for 12 hours. At this time, a disc-shaped permanent magnet having a diameter of 20 mm was attached to a specific site on the outer surface of the plastic culture container, and the luciferase gene to the smooth muscle cells at the site where the magnet was attached and the site where no magnet was attached. The introduction efficiency of was investigated. That is, the gene transfer efficiency was calculated by measuring the bioluminescence obtained by expressing the gene. Luminescence was corrected by the total protein concentration in the total smooth muscle cell lysate. The result is shown in FIG. In the figure, MF (+) indicates the part where magnetic induction is performed by attaching a magnet, and MF (-) indicates the measurement result at the part where such magnet is not attached.

Comparative Example 1 As a control, an attempt was made to introduce the luciferase gene into smooth muscle cells in the same manner as in Example 1 except that the liposome containing only the luciferase gene was used without using the magnetosome. Then, the efficiency of introduction of the gene into smooth muscle cells was measured in the same manner as above. The results are also shown in Fig. 1 (Note: RLV = Relative Light Unit). As can be seen from the results in FIG. 1, in the case of Comparative Example 1 in which the liposome containing no magnetosome was used, the luciferase gene transfer efficiency was the same regardless of the application of the magnetic field. However, in the case of Example 1 in which the liposome containing magnetosomes was used, the introduction efficiency increased more than 10 times when the magnetic field was applied as compared with the case where the magnetic field was not applied.

Example 2 Similar to the one used in Example 1, a liposome containing a magnetosome and a luciferase gene was prepared. This was added to a plastic culture container in which smooth muscle cells were cultured in the same manner as in Example 1 and reacted. This operation was performed for a case where a permanent magnet was arranged on the entire bottom surface of the plastic culture container and a case where such a permanent magnet was not arranged at all. The efficiency of luciferase gene introduction into smooth muscle cells was measured over time from the expression of the gene. The result is shown in Figure 2.
Shown in. In the figure, MI (+) is the case where magnetic induction is performed using a magnet, and MI (-) is the case where such magnetic induction is not performed. As can be seen from the results of FIG. 2, the introduction efficiency was almost saturated at 1 minute after administration of the liposome at the site where magnetic induction was performed, but until the same introduction efficiency was obtained at the site where magnetic induction was not performed. It took more than 60 minutes.

[0012]

INDUSTRIAL APPLICABILITY The liposome containing a magnetic bacterium magnetosome and a gene of the present invention is a novel substance, and a desired gene can be introduced into a target cell with high efficiency and in a short time by applying a magnetic field. Since the liposome can be magnetically induced, it is possible to selectively introduce a gene into a target cell.

[Brief description of drawings]

FIG. 1 shows the results of measuring the gene transfer efficiency at the site where magnetic induction was performed and the site where magnetic induction was not performed using the liposome containing the magnetosome of Example 1 and the liposome not containing the magnetosome of Comparative Example 1, respectively. FIG.

FIG. 2 is a graph showing the results obtained by measuring the effect of magnetic induction on the gene transfer efficiency obtained in Example 2 over time.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (71) Applicant 594053420 Koichiro Harada 322 Zeno-cho, Kashihara-shi, Nara (72) Inventor Koichiro Harada 322 Zeno-cho, Kashihara-shi, Nara (72) Ryozo Nagai Yushima, Bunkyo-ku, Tokyo 4-8-3 Nissho Iwai Daini Hongo Mansion 609 (72) Inventor Megumi Matsunaga 2-41-13 Sachimachi, Fuchu-shi, Tokyo Fuchu Third House 2-304

Claims (2)

[Claims] 1. A liposome containing a magnetosome of a magnetic bacterium and a gene. 2. A method for introducing a gene into a cell, which comprises a step of inducing the liposome into a cell by applying a magnetic field to the liposome containing the magnetosome of a magnetic bacterium and the gene, and bringing the liposome into contact with the cell.