JPH07213597A - Twisted yarn of purified collagen-like substance, formed body of twisted yarn and their manufacture - Google Patents

Abstract

PURPOSE:To provide twisted yarn of purified collagen-like substance which has superior biocompatibility and mechanical strength, is substitutable with tissue and an internal organ at an affected part of a human body as time passes after application, and can control in vivo decomposability. CONSTITUTION:A thin and long piece of purified collagen-like substance in wet state obtained from human amnions or human villi is dried and twisted, and cross-linked and/or succinylated if necessary, then is formed into a sheet or a tube to be a formed body. Or a film of purified collagen-like substance obtained from human amnions or human villi is laminated on the formed body such as sheet and tube formed from the twisted yarn.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a twisted yarn of a purified collagen-like substance, a molded article produced from this twisted yarn, and a method for producing the same. More specifically, it is excellent in biocompatibility and mechanical strength, and after application, It replaces the tissues and organs of the affected part of the human body over time, and promotes and assists the repair of diseased parts and damaged parts with its own tissues and organs.After self-repair is completed, decomposition, metabolism, and excretion from the body And biodegradability can be controlled to desired conditions, and artificial biomaterials such as sutures, wound protectors, wound prostheses, wound prostheses, and adhesion prevention materials, artificial blood vessels, artificial trachea, artificial TECHNICAL FIELD The present invention relates to a twisted yarn of a purified collagen-like substance used as an artificial organ such as an esophagus, an artificial urethra, an artificial ureter, an artificial skin, a molded product produced from the twisted yarn, and a method for producing these.

[0002]

2. Description of the Related Art A wound protective material used for healing an injured portion of a living organ or a living tissue, a prosthesis material or a filling material for a living tissue or a living organ at a diseased portion which is lost by surgery. , Synthetic artificial materials such as bioartificial materials such as sutures for suturing surgical incisions or artificial blood vessels, artificial trachea, artificial esophagus, artificial urethra, artificial ureters, artificial skin, etc. are used. Manufactured from bio-derived materials.

[0003] These bioartificial materials and artificial organs are required to be biocompatible, safe and effective, and within a desired period after application, the living tissue or living body of the affected area is required. While regenerating and repairing organs, in vivo decomposition, metabolism,
It is preferably excreted and finally has a function of self-repairing by substituting the living tissue or organ of the affected area, and in this respect, the synthetic polymer material is assimilated with the tissue or organ of the affected area,
There is a problem of lack of biocompatibility, such as non-engraftment, keloidization or deformity of the application site, or various inconvenient symptoms caused by the fact that it permanently remains in the living body.

On the other hand, as biological materials, pig, cow,
Using tissue and organs derived from xenogeneic animals such as horses as raw materials, artificial wound materials derived from pig skin, wound prostheses derived from equine pericardium, sutures derived from sheep's intestine, and artificial materials derived from porcine heart aortic valve. Artificial organs such as biological valves and artificial blood vessels derived from bovine carotid arteries have been researched and developed, and most of them are used for medical purposes. However, since it uses a tissue or organ derived from a different animal and lacks biocompatibility such as a different biological reaction, glutaraldehyde cross-linking, chemical modification such as succinylation treatment, heat cross-linking, or UV irradiation, Physical modification such as cross-linking treatment by electron beam irradiation or radiation irradiation is required, and biodegradability and substitutability are lost due to these chemical modification and physical modification. That is, since it remains as a foreign body in the patient's living body even after treatment,
Since the same problem as in the case of the synthetic polymer material occurs, it may be necessary to replace it with a new bioartificial material or artificial organ by reoperation in some cases.

Further, as a biologically derived material, a wound replacement material of the same type using human dura mater or human fascia lata as a raw material is used. However, these bioartificial materials can be formed only in a sheet shape, and cannot be formed in other shapes. Further, since human dura mater and human fascia lata are collected only from human corpses, the supply amount is extremely low and extremely expensive, and there is a medical practice problem such as social belief.

[0006]

DISCLOSURE OF THE INVENTION The inventors of the present invention have conducted extensive studies to solve the above-mentioned problems, and as a result, obtained from human amniotic membrane or human chorion constituting human fetal membrane to be disposed of as medical waste. When the purified collagen-like substance in a wet state is twisted while being dried, the twist does not return even if it is wet again, and excellent mechanical strength is maintained. Furthermore, the twisted yarn can be formed into a sheet or a tube. Found that the obtained molded body also has excellent mechanical strength,
The present invention has been completed. That is, the present invention is excellent in biocompatibility and mechanical strength, and after application, it is replaced with a tissue or an organ of a human living body affected area over time to promote repair of a diseased portion or a damaged portion with its own tissue or organ. Provided are a twisted yarn of a purified collagen-like substance which can be decomposed, metabolized and excreted from the living body after completion of self-repair, and whose biodegradability can be controlled, a molded article produced from this twisted yarn, and a method for producing these. The purpose is to do.

[0007]

Means for Solving the Problems That is, the present invention provides a twisted yarn obtained by twisting a strip of a purified collagen-like substance obtained from human amnion or human chorion; a strip of the purified collagen-like substance in a wet state. A twisted yarn which is twisted while being dried, and optionally subjected to a cross-linking treatment and / or a succinylation treatment; a molded article obtained by molding the twisted yarn into a sheet or a tube; Alternatively, a method for producing a molded product, which is formed into a tubular shape, and then optionally subjected to a crosslinking treatment and / or a succinylation treatment, and then dried; on the surface of the molded product, obtained from human amniotic membrane or human chorion membrane A molded article in which a membrane of a purified collagen-like substance is laminated; and a membrane of a purified collagen-like substance obtained from human amniotic membrane or human chorion is laminated on the surface of the molded article, and if necessary. It was subjected to a crosslinking treatment and / or succinylated process, a process for producing a molded article to be dried.

The purified collagen-like substance used in the present invention is
It consists only of a compact layer obtained by removing the epithelial layer, basement membrane layer and fibroblast layer from human amniotic membrane or human chorion by the treatment described below. Dense layers are human type I, type III, type V and X
Since it contains VI type collagen as a main component, it has excellent properties and biocompatibility suitable as a substrate material for proliferating biological membranes and fibroblasts, and also has suitable mechanical strength.

The purified collagen-like substance is manufactured as follows. First, only the fetal membrane is separated from the integrated human fetal membrane, placenta and umbilical cord immediately after the delivery of an uninfected mother, and the amniotic membrane, chorion, and the capsular membrane are separated in a benzalkonium chloride solution or a benzalkonium bromide solution. The amnion and chorion were separated from the fetal membrane consisting of four layers, the bursa decidua and the floor decidua, and then treated with enzyme using ficin, and further subjected to ultrasonic cleaning treatment to leave the remaining epithelial layer, basement membrane layer and A purified collagen-like substance which is a sterile, virus-free, foreign-body-free and pyrogen-free substance consisting of a dense layer containing human type I, type III, type V and type XVI collagen as a main component by removing the fibroblast layer To get the film of.

The twisted yarn of the present invention is obtained by twisting one elongated piece of a purified collagen-like substance, twisting or knitting two or more elongated pieces, or further combining two or more of these pieces. It may be twisted or braided, or braided, and the cross-sectional shape thereof may be substantially circular or flat. The number of twists depends on the type of bioartificial material or artificial organ produced from the twisted yarn, but is preferably 2 to 20 turns / cm. 2 times /
If it is less than cm, the twist may return, and if it exceeds 20 times / cm, the twisted yarn may be cut. The larger the number of twists, the higher the tensile strength and the slower the biodegradation rate becomes. For example, 1 when used as a suture
0 times / cm or more, 5 times / cm when used as a damage protection material
It is preferably cm.

The diameter of the twisted thread can be appropriately determined depending on the use of the artificial material for living body or artificial organ, and is not particularly limited. For example, when it is used as a suture thread, the diameter of a suture thread which is usually used. Same as 0.33mm (No. 0)
.About.1.016 mm (No. 7) is preferable. In the case of a twisted yarn having a flat cross section, the diameter of a circle having the same area as the cross sectional area is the diameter of the twisted yarn. When the twisted yarn is dried, it firmly adheres to the collagen-like substances and becomes excellent in mechanical strength such as tensile strength. Although it becomes swollen and flexible even in a wet state, the twist does not return. No, retains the twisted state and excellent mechanical strength.

The twisted yarn of the purified collagen-like substance of the present invention is manufactured as follows. First, since the membrane of the above-mentioned purified collagen-like substance contains elastin, it is extremely flexible and elastic in a wet state, and it cannot be rolled up and held in a stable state immediately. Therefore, a solution of benzalkonium chloride or benzamide bromide is used. In a ruconium solution, stretch it so that it does not have wrinkles, place it on a support and fix it, then keep it in a stream of dry air or dry nitrogen gas, or keep it under reduced pressure. Dry to a semi-dry state where there are no water droplets on the surface. If water droplets are present on the surface of the purified collagen-like substance, it will not be possible to maintain a stable shape, and the cutting operation described below will be difficult, while drying until the water retained by the purified collagen-like substance is completely removed, This is because when shear stress is applied, a crack is generated and the crack expands rapidly.

Next, the purified collagen material fixed on the support is cut into strips with a laser beam, heat rays and a sharp blade in a sterile condition. Then, the obtained strips of collagen-like substance are sprayed or applied with purified water, or immersed in purified water to be in a wet state, and twisted while being pulled by an appropriate force. The cutting width and the tension for twisting may be appropriately determined according to the desired diameter of the twisted yarn. At this time, if water is excessively absorbed, the collagen-like substance cannot retain the shape of the strip and is curled, which is not preferable. When connecting the strips of collagen-like substance, overlap the ends of the strips of collagen-like substance with the ends of the strips of other collagen-like substances by absorbing excessive moisture, and twist them while crimping. Good. Then, the yarn is maintained in a stream of dry air or dry nitrogen gas, or is dried under reduced pressure to obtain a twisted yarn of a purified collagen-like substance having a substantially circular cross section. In the case of producing a flat twisted yarn, the strips of the collagen-like substance in a twisted state before drying or the obtained twisted yarn are made to absorb moisture to have flexibility, and then pressed while being heated. And dry it.

The molded article of the present invention is a woven fabric such as a plain weave or a twill weave produced from a twisted yarn of the above purified collagen-like substance,
A sheet-shaped molded product having holes or gaps such as a knitted fabric or a mesh sheet; a tubular molded product having holes or gaps formed by weaving a twisted yarn in a cylindrical shape with a rod or the like as a core material, knitting, or spirally winding; A film of the above-mentioned purified collagen-like substance is laminated on one surface or both surfaces of the sheet-shaped molded product having pores or gaps, or on the inner surface and / or the outer surface of the tubular molded product having pores or gaps, and if necessary gelatin. It is a sheet-shaped or tube-shaped molded body having no holes or gaps, which are integrally fixed with an adhesive such as a solution or a collagen solution. The sizes of the holes and the gaps of the sheet-shaped or tube-shaped molded product having the above-mentioned holes or gaps are not particularly limited because they vary depending on the bioartificial material or artificial organ in which the molded product is used.

As the gelatin used as the raw material of the gelatin solution of the adhesive, general medical purified gelatin can be used, and as the collagen used as the raw material of the collagen solution, human-derived collagen is used. The concentration of the gelatin solution is 1.0 to 5.0% by weight, preferably 2.0 to 3.0.
The concentration of the collagen solution by weight is 0.001 to 3.0% by weight, preferably 0.002 to 3.0% by weight.

The molded product of the present invention is manufactured as follows. The method for producing a sheet-shaped or tube-shaped molded product having pores or gaps is as follows. First, the twisted yarn of the above-mentioned dried and purified collagen-like substance is sprayed or applied with purified water, or immersed in purified water to absorb water and soften. Property, and then woven in a sheet form into a plain weave or a twill weave by a known method, knitted, or made into a mesh sheet, or woven by twisting a twisted yarn in a cylindrical shape around a core material such as a rod into a woven fabric, and knitting. After knitting, making a mesh, or winding it in a spiral,
It is kept in a stream of dry air or dry nitrogen gas or dried under reduced pressure.

The sheet-shaped or tube-shaped molded product having no holes or gaps can be produced by applying the above-mentioned adhesive to the surface of the sheet-shaped or tube-shaped molded product having the above-mentioned holes or gaps, or molding these, if necessary. After immersing the body in the adhesive, a film of the purified collagen-like substance is provided on one or both sides of the sheet-shaped molded product having pores or gaps, or on the inner surface of the tubular molded product having pores or gaps and / or Alternatively, it is laminated on the outer surface and then dried under the above drying conditions. It should be noted that the sheet-shaped or tube-shaped molded product having pores or gaps and the film of the purified collagen-like substance adhere to each other when dried, but if the adhesion is insufficient, the above-mentioned adhesive is applied to the surface of these molded products as necessary. After coating or immersing these molded bodies in the adhesive, a film of purified collagen-like substance may be laminated and dried.

The twisted yarn composed of the purified collagen-like substance of the present invention and the molded article produced from the twisted yarn are the woven fabric of the purified collagen-like substance which controls the biodegradability of the purified collagen-like substance and is adhered to each other with an adhesive. A cross-linking treatment is performed to integrally fix the knitted fabric, mesh sheet, etc. and the film of the purified collagen-like substance. In particular, when all the amino groups in the collagen molecule are subjected to a crosslinking reaction, the twisted yarn and the molded article produced from this twisted yarn can be made completely free of antigenicity and non-degradable and absorbable in vivo.

Examples of the cross-linking treatment include glutaraldehyde cross-linking, thermal cross-linking, cross-linking by gamma ray irradiation, electron beam irradiation, and ultraviolet ray irradiation. Glutaraldehyde cross-linking
Concentration 0.03 to 3.0% by weight, preferably 0.05 to
It may be soaked in a 2.0% by weight glutaraldehyde solution and then air dried. This is because when the concentration is less than 0.03% by weight, the collagen-like substance is easily peeled off, and when it exceeds 3.0% by weight, the collagen-like substance is hardened and loses biocompatibility. It is considered that the glutaraldehyde cross-linking proceeds when the amino group in the collagen molecule reacts with the aldehyde group of glutaraldehyde.

The thermal crosslinking may be carried out by heating in vacuum to 90 to 200 ° C., preferably 105 to 150 ° C., dehydration and crosslinking. The heating time is appropriately adjusted depending on the heating temperature, the degree of pressure reduction, the desired degree of crosslinking, etc., but is usually about 6 to 24 hours. It is considered that the thermal cross-linking of collagen mainly proceeds by Schiff base formation, aldol condensation or the like between sugar chains in the collagen molecule or aldehyde groups generated by oxidation and lysine or hydroxylysine in the collagen molecule.

Crosslinking by irradiation with gamma rays, electron beams, ultraviolet rays, etc. is carried out in an atmosphere having a humidity of 75% or more. This is because the purified collagen-like substance is decomposed when gamma rays, electron beams, ultraviolet rays, etc. are irradiated at a humidity of less than 75%. The water content of the purified collagen-like substance should be 20% or more, depending on the dose. Water content is 2
When it is less than 0%, the decomposition of the collagen-like substance has priority over the crosslinking. The dose of electron beam and radiation is
1 × 10 ⁶ to 1 × 10 ⁷ roentgen is preferable. 10 ⁶
This is because if it is less than roentgen, the cross-linking of the purified collagen-like substance is insufficient, and if it exceeds 10 ⁷ roentgen, the purified collagen-like substance is decomposed much. The irradiation amount of ultraviolet rays is determined by the irradiation distance and the irradiation output, but irradiation with an ultraviolet lamp of approximately 4 watts from a distance of 10 cm for 1.5 hours is preferable.

Further, the twisted yarn of the present invention and the molded product produced from the twisted yarn can be subjected to succinylation treatment in order to impart anticoagulant property and hemolytic property and remove antigenicity.
Succinylation causes most of the remaining amino groups in the collagen molecule to react with succinic anhydride. This succinylation may be performed by a commonly used method, for example,
1 to 48 of a mixture of 250 ml of 0.02M borate buffer (pH 9.0) and 50 ml of 5% acetone solution of succinic anhydride.
After dipping for a time, preferably 12 to 24 hours, it may be washed with water and then dried under reduced pressure. In order to confirm that the collagen-like substance is succinylated, the succinylated twisted yarn and the molded body may be immersed in a 0.33 wt% ninhydrin aqueous solution for 3 to 5 minutes. Since the color reaction of ninhydrin is based on the condensation product generated by the reaction between the amino group in collagen and ninhydrin, the more succinylated, the less likely the collagen-like membrane is stained.

The twisted yarn of the purified collagen-like substance of the present invention produced by the above steps can be applied to sutures and the like, and the molded article produced from the twisted yarn is a wound protection material embedded in a living body. , Wound prosthesis, wound prosthesis,
It can be applied to artificial materials for living organisms such as anti-adhesion materials or artificial blood vessels, artificial trachea, artificial esophagus, artificial urethra, artificial ureters, artificial organs such as artificial skin, and the like.

[0024]

EXAMPLES Next, the present invention will be described in more detail based on examples. Example 1 Production of Purified Collagen-Like Substance Using a sharp knife from the fetal membrane, placenta, and umbilical cord excreted immediately after delivery from a mother confirmed to have not been infected with pathogens such as hepatitis, virus such as HIV, and sexually transmitted diseases. Blood that adheres to the fetal membrane in the running water of physiological saline of Japan Pharmacopeia after being immersed in 2 liters of 0.1% Japanese Pharmacopoeia / benzalkonium chloride solution immediately after removing and separating only the fetal membrane Was thoroughly washed and removed, and the obtained fetal membrane was immersed in 2 liters of a new 0.1% Japanese Pharmacopoeia benzalkonium chloride solution. The fetal membrane obtained was in a substantially cylindrical shape consisting of four layers of amniotic membrane, chorion, encapsulating decidua and floor wall decidua. Then, in a new 0.1% benzalkonium chloride solution, a cylindrical fetal membrane was incised with a sharp blade to make it flat, and then 10 liters of a new 0.1% Japanese Pharmacopoeia benzalcochloride. The amniotic membrane was peeled and separated by gently shaking it in a nitric acid solution so as to shake it. Most of the chorion was peeled from the residual membrane, so the chorion was completely peeled and separated from the residual membrane with a finger. The separated amnion and chorion were immersed in another fresh 0.1% benzalkonium chloride.

Then, the amniotic membrane obtained by the above method was stretched and developed on a work bench made of high-density polyethylene, and then fixed on the work bench using two frames of sterile polypropylene for medical use, and aseptic pyrogen. 60 in free running water
Ultrasonic cleaning (frequency 20 to 50 kHz) was performed for a minute. Then, the ultrasonically washed amniotic membrane was immersed in a 0.01% ficin pH 7.4, 0.2M phosphate buffer solution (Ficin preparation solution) in which 2% NaN ₃ was dissolved at room temperature for 24 hours.
The amniotic membrane after the ficin treatment was subjected to ultrasonic cleaning (frequency 20 to 50 kHz) again for 60 minutes. The weight of the amniotic membrane was reduced by 23% as compared with before the two ultrasonic cleanings. The amniotic membrane is a purified collagen-like substance composed of a dense layer of human amniotic membrane, which is obtained by removing the part of the surface layer of the collagen-like membrane that is a substance derived from living tissue or a human amniotic membrane substrate substance attached to the amniotic membrane by two ultrasonic cleanings. A film of was obtained. This purified collagen-like substance was tested for sterility, pyrogen test method, foreign body observation test method, etc. defined in the approval criteria for artificial blood vessel production of the Japanese Pharmacopoeia, and as a result, it was confirmed that there is no sterility, pyrogen and foreign material. confirmed.

Manufacture of a twisted yarn having a substantially circular cross section . The purified collagen-like substance obtained next was placed in a sterile booth for 3 times.
It was left for 72 hours in a sterile air stream at 0 ° C. and dried until there were no water droplets on the surface. Then, the obtained purified collagen-like substance in a semi-dried state was cut with a sharp blade in a sterile state to obtain a strip of the purified collagen-like substance having a width of 1 cm and a length of 15 cm. Next, purified water is sprayed onto the obtained strips of the purified collagen-like substance for 1 minute so as to be in a wet state, then removed from the frame, and one strip is twisted to form a twisted yarn. Collagen-like membranes were overlapped and twisted to produce a twisted yarn having a diameter of 2 mm and a length of 54 cm, which had a substantially circular cross section. The number of twists of the twisted yarn was 5 times / cm. Then, it was dried in a sterile air stream at 60 ° C. for 3 minutes to produce a yarn of purified collagen-like substance. The obtained twisted yarn was excellent in mechanical strength such as tensile strength and was a rigid twisted yarn.
The twist did not return even in the wet state, and it had excellent mechanical strength.

Manufacture of a twisted yarn having a flat cross section Further, after the above twisted yarn is sprayed with purified water to make it wet,
After pressing while heating to 130 ° C., it was dried in a sterile air stream at 60 ° C. for 3 minutes to produce a flat twisted yarn made of a purified collagen-like substance. The obtained twisted yarn was excellent in mechanical strength such as tensile strength like the twisted yarn having a substantially circular cross section, and was a rigid twisted yarn. The twist did not return even in a wet state, and it had excellent mechanical strength.

Manufacture of a sheet-shaped molded product having no pores The obtained twisted yarn having a substantially circular cross section is treated with water at 30 ° C. for 10 times.
After spraying for a minute to soften the fabric, it was woven into a plain weave fabric.
On the obtained woven fabric (10 cm x 10 cm) having an average pore diameter of 2 mm,
After applying a 0.002% by weight collagen aqueous solution, a film of the above-mentioned purified collagen-like substance (10 cm × 10 cm) was attached, and it was further dried for 24 hours under hot dry air at 37 ° C. Next, this was immersed in a 0.05% by weight aqueous solution of glutaraldehyde for 10 minutes and allowed to stand, and then unreacted glutaraldehyde solution was washed and removed using purified water. The obtained sheet was immersed in a 0.33 wt% ninhydrin aqueous solution, and it was confirmed that the amino groups in collagen remained and there was room for succinylation.

Manufacture of a tubular molded article with a gap The surface of a medical polypropylene rod having a diameter of 5 mm was coated with Japanese Pharmacopoeia glycerin and sprayed with water at 30 ° C., and the cross section was made substantially with the rod as a core. 1mm of the above round yarn
After wrapping with a gap between them, it is dried in a sterile air stream at 60 ° C for 5 minutes, the core is removed, and the inner diameter is 5 mm, the thickness is 2 mm,
A tubular molded body having a gap of 1 mm was obtained.

Manufacture of tube-shaped molded product having no gap The sheet-shaped molded product having no hole produced by the above-mentioned method is cut with a sharp blade in an aseptic state to obtain a strip having a width of 5 mm, and the strip is provided with the above-mentioned gap. In the same manner as in the method for producing a tubular molded body having
A tubular molded body having a thickness of 2 mm and having no gap was obtained.

Improvement of biocompatibility A twisted yarn having a circular cross section, a twisted yarn having a flat cross section, a sheet-shaped molded product having no holes, a tube-shaped molded product having a gap and a tube-shaped molded product having no gap produced by the above method. To 0.
A succinylation treatment was carried out by immersing in a mixed solution of 500 ml of a borate buffer solution of 02M, pH 9.0 and 100 ml of a 5% acetone succinic anhydride solution for 4 to 8 hours. The succinylated material was immersed in a 0.33% aqueous ninhydrin solution to confirm that there were no residual amino groups in the collagen (improved biocompatibility).

Sterilization A twisted yarn having a substantially circular cross section, a twisted yarn having a flat cross section, a sheet-shaped molded product having no pores, a tubular molded product having a gap and a tubular molded product having no gap produced by the above-mentioned method are treated with ethylene oxide. Using a mixed gas of 20% gas and 80% carbon dioxide gas, it was sterilized for 7 hours under the conditions of 50 ° C. and 40% humidity. It was also sterilized by irradiation with 2.0 megarads of gamma rays. After sterilization, as a result of testing according to the Japanese Pharmacopoeia sterility test method and pyrogen test method, it was confirmed that all the twisted yarns and molded articles had no change in physical properties, were sterile, and had no pyrogen.

[0033]

EFFECTS OF THE INVENTION When the twisted yarn of the present invention is dried, it firmly adheres to the purified collagen-like substance, and even if it is made wet to have flexibility, the twist does not return. Twisted yarns and molded articles are excellent in biocompatibility, mechanical strength such as tensile strength, and are replaced with tissues or organs of a human body affected area over time due to decomposition in vivo after application, and sutured portions, diseased portions or It promotes and assists the damaged area to repair with its own organs and tissues, and is decomposed, metabolized, and excreted from the body after completion of self-repair. Further, the degree of cross-linking can be adjusted by the cross-linking treatment to control the biodegradability to desired conditions. Furthermore, succinylation can impart anticoagulant and hemolytic properties. Since the molded product is manufactured using the twisted yarn of the present invention having excellent mechanical strength, it has excellent mechanical strength without using a reinforcing material such as a synthetic polymer material. Therefore, sutures, wound protectors,
The present invention can be applied to artificial materials for living bodies such as wound filling materials, wound prosthesis materials, and adhesion preventing materials, or artificial blood vessels, artificial trachea, artificial esophagus, artificial urethra, artificial ureters, artificial organs such as artificial skin, and the like.

Claims (6)

[Claims] 1. A twisted yarn obtained by twisting a strip of a purified collagen-like substance obtained from human amnion or human chorion. 2. A strip of a purified collagen-like substance in a wet state obtained from human amniotic membrane or human chorion is twisted while being dried, and is optionally subjected to a crosslinking treatment and / or a succinylation treatment. Manufacturing method of twisted yarn. 3. A molded product obtained by molding the twisted yarn according to claim 1 into a sheet shape or a tube shape. 4. A method in which the twisted yarn according to claim 1 is moistened, shaped into a sheet or a tube, and optionally crosslinked and / or succinylated, and then dried. A method for manufacturing a featured molded article. 5. A molded product obtained by laminating a film of a purified collagen-like substance obtained from human amnion or human chorion on the surface of the molded product obtained by molding the twisted yarn according to claim 1 into a sheet or tube. 6. A layer of purified collagen-like substance obtained from human amniotic membrane or human chorion is laminated on the surface of a molded body obtained by molding the twisted yarn according to claim 1 into a sheet or tube, and then, if necessary. A method for producing a molded article, which comprises subjecting the material to a crosslinking treatment and / or a succinylation treatment, and then drying.