JPH07198572A - Temperature agitator for enzyme immunoreaction assay - Google Patents
Temperature agitator for enzyme immunoreaction assayInfo
- Publication number
- JPH07198572A JPH07198572A JP5351588A JP35158893A JPH07198572A JP H07198572 A JPH07198572 A JP H07198572A JP 5351588 A JP5351588 A JP 5351588A JP 35158893 A JP35158893 A JP 35158893A JP H07198572 A JPH07198572 A JP H07198572A
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- Prior art keywords
- sample
- heated
- microwave
- heating
- stirring device
- Prior art date
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Abstract
Description
【０００１】[0001]
【産業上の利用分野】この発明は、医薬分野における酵
素免疫反応測定に用いられるものであって、被加熱試料
を均一に加熱するための温度撹拌装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a temperature stirring device for uniformly heating a sample to be heated, which is used for enzyme immunoassay measurement in the field of medicine.
【０００２】[0002]
【従来の技術】酵素免疫反応測定は次の手順で行われ
る。検体希釈→検体分注（温度撹拌・洗浄）→標
識抗体液添加（温度撹拌・洗浄）→発色試色添加（温
度撹拌）→反応停止液添加→吸光度測定→濃度算
出2. Description of the Related Art The enzyme immunoassay measurement is performed by the following procedure. Specimen dilution → Specimen dispensing (Temperature stirring / washing) → Labeled antibody solution addition (Temperature stirring / washing) → Color development test color addition (Temperature stirring) → Reaction stop solution addition → Absorbance measurement → Concentration calculation
【０００３】このように、酵素免疫反応測定では、三回
の温度撹拌工程（インキュベーション）が必要となる。
従来、この温度撹拌工程では、発熱抵抗体を用いた抵抗
加熱手段と、Ｘ−Ｙテーブル等の撹拌手段とから構成さ
れる温度撹拌装置が用いられている。As described above, the enzyme immunoreaction measurement requires three temperature stirring steps (incubation).
Conventionally, in this temperature stirring step, a temperature stirring device including a resistance heating means using a heating resistor and a stirring means such as an XY table is used.
【０００４】酵素免疫反応測定には、多数の有底穴（ウ
ェル）を有する試料保持平板（マイクロプレート）が用
いられる。そして、これらの多数の有底穴のそれぞれ
に、酵素免疫反応測定用の被加熱試料を入れて、前記の
温度撹拌装置で各被加熱試料を均一に加熱する。A sample holding plate (microplate) having a large number of bottomed holes (wells) is used for the enzyme immunoassay measurement. Then, the heated sample for measuring the enzyme immunoreaction is put into each of these many bottomed holes, and each heated sample is uniformly heated by the temperature stirring device.
【０００５】[0005]
【発明が解決しようとする課題】このとき、温度撹拌装
置には、各被加熱試料間で温度のバラツキ（以下、「加
熱むら」という。）が生じないように、加熱することが
要求される。そのため、加熱に要する時間が非常に長く
なり、被加熱試料（抗体）の種類によっては数十時間も
かかることがあった。At this time, the temperature stirring device is required to heat the sample to be heated so that there is no variation in temperature between the samples to be heated (hereinafter referred to as "heating unevenness"). . Therefore, the time required for heating becomes very long, and it may take several tens of hours depending on the type of sample (antibody) to be heated.
【０００６】そこで、本発明者は、迅速かつ均一な加熱
を実現するために、マイクロ波加熱を用いて被加熱試料
の内部から加熱することを考えた。すなわち、温度撹拌
装置として家庭用電子レンジを用いて、被加熱試料を加
熱することを試みた。ところが、被加熱試料は、最高と
最低とで30℃程度の温度差という、極端な加熱むらを生
じた。したがって、家庭用電子レンジを、酵素免疫反応
測定用の温度撹拌装置として用いることはできないこと
が判明した。Therefore, the present inventor has considered heating the sample to be heated from the inside by using microwave heating in order to realize rapid and uniform heating. That is, an attempt was made to heat the sample to be heated using a household microwave oven as a temperature stirring device. However, in the sample to be heated, a temperature difference of about 30 ° C. between the highest temperature and the lowest temperature caused an extreme uneven heating. Therefore, it was found that the household microwave oven cannot be used as a temperature stirring device for measuring enzyme immunoreaction.
【０００７】[0007]
【発明の目的】本発明の目的は、マイクロ波を用いて迅
速かつ均一な加熱を実現した、酵素免疫反応測定用の温
度撹拌装置を提供することにある。It is an object of the present invention to provide a temperature stirring device for measuring enzyme immunoreaction, which realizes rapid and uniform heating using microwaves.
【０００８】[0008]
【課題を解決するための手段】本発明者は、温度撹拌装
置として家庭用電子レンジを用いた場合の加熱むらの原
因を究明するために、試料保持平板における各被加熱試
料の温度分布を、図５に示す三次元グラフに表してみ
た。図５において、試料保持平板には、一定間隔９mmで
８行（Ａ〜Ｈ）×12列（１〜12）の有底穴が形成されて
おり、各有底穴に被加熱試料が入れられている。Means for Solving the Problems In order to investigate the cause of uneven heating when a household microwave oven is used as a temperature agitator, the present inventor determined the temperature distribution of each heated sample on a sample holding plate as follows. This is shown in the three-dimensional graph shown in FIG. In FIG. 5, the sample-holding flat plate is formed with bottomed holes in 8 rows (A to H) × 12 columns (1 to 12) at regular intervals of 9 mm, and the sample to be heated is put in each bottomed hole. ing.
【０００９】ここで、本発明者は、図５に示す現象を考
察することにより、被加熱試料の温度分布が家庭用電子
レンジのマイクロ波の波長に一致していることを見い出
した。以下に、詳しく説明する。Here, the present inventor has found that the temperature distribution of the sample to be heated matches the wavelength of the microwave of the household microwave oven by considering the phenomenon shown in FIG. The details will be described below.
【００１０】マイクロ波は、電磁波であるから次式が成
り立つ。Since the microwave is an electromagnetic wave, the following equation holds.
【００１１】ｃ＝ｆλ ・・・ C = fλ ...
【００１２】ここで、ｃ：光の速さ，ｆ：周波数，λ：
波長である。Here, c: speed of light, f: frequency, λ:
Wavelength.
【００１３】したがって、式により、家庭用電子レン
ジのマイクロ波は、周波数が2450MHz であるから波長が
122 mmとなる。Therefore, according to the formula, the microwave of a household microwave oven has a frequency of 2450 MHz, and therefore has a wavelength of
It will be 122 mm.
【００１４】一方、図５に示すように、Ｘ方向の温度に
おいて、最高値はＤ行及びＥ行に生じ、最低値はＡ行，
Ｈ行に生じている。また、Ｙ方向の温度において、最高
値は４列，９列に生じ、最低値は１列，６列及び７列，
12列に生じている。On the other hand, as shown in FIG. 5, at the temperature in the X direction, the highest values occur in the D and E rows, and the lowest values in the A row,
It occurs in line H. Further, in the temperature in the Y direction, the highest values occur in the 4th and 9th rows, and the lowest values occur in the 1st, 6th and 7th rows,
It occurs in 12 rows.
【００１５】さて、図４は、図５の被加熱試料の温度分
布とマイクロ波との関係を示す斜視図である。図４にお
いて、試料保持平板１２には、一定間隔９mmで８行×12
列の有底穴１４が形成されている。ここで、波形ｍ２ｘ
は、波長が122 mmのマイクロ波のＸ方向成分であり、Ｚ
方向を電界強度の絶対値としている。そして、電界強度
の最高値（★印）を、図５におけるＸ方向の温度が最高
となるＤ行及びＥ行に合わせたものである。同様に、波
形ｍ２ｙは、波長が122 mmのマイクロ波のＹ方向成分で
あり、電界強度の最高値（★印）を、図５におけるＹ方
向の温度が最高値となる４列，９列に合わせたものであ
る。Now, FIG. 4 is a perspective view showing the relationship between the temperature distribution of the sample to be heated in FIG. 5 and the microwave. In FIG. 4, the sample holding flat plate 12 has 8 rows × 12 at regular intervals of 9 mm.
Rows of bottomed holes 14 are formed. Here, the waveform m2x
Is the component of the microwave in the X direction with a wavelength of 122 mm, and Z
The direction is the absolute value of the electric field strength. Then, the maximum value of the electric field intensity (marked with *) is adjusted to the D and E rows where the temperature in the X direction is the highest in FIG. Similarly, the waveform m2y is the Y-direction component of the microwave having a wavelength of 122 mm, and the maximum value of the electric field strength (marked with *) is shown in columns 4 and 9 where the temperature in the Y direction is the maximum value in FIG. It is a combination.
【００１６】このように、被加熱試料の温度分布がマイ
クロ波の波長に極めて良く一致する。すなわち、定在波
化したマイクロ波の電界強度の空間分布が、そのまま被
加熱試料の温度分布となっていると考えられる。また、
電界強度Ｅと、単位体積当たりの消費電力ｐ（発熱量）
とは、次式の関係にある。In this way, the temperature distribution of the sample to be heated matches the wavelength of the microwave very well. That is, it is considered that the spatial distribution of the electric field strength of the microwave that has been converted into the standing wave is the temperature distribution of the sample to be heated as it is. Also,
Electric field intensity E and power consumption p (heat generation amount) per unit volume
And are in the relationship of the following formula.
【００１７】 ｐ＝(5/9×10-1 0)ｆ・Ｅ2 ・εｒ・ tanδ ・・・ [0017] p = (5/9 × 10 -1 0) f · E 2 · εr · tanδ ···
【００１８】ここで、εｒ・ tanδは、誘電係数であっ
て、物質によって定まる定数である。Here, εr · tanδ is a dielectric constant, which is a constant determined by the substance.
【００１９】式に示されるように、熱エネルギは、電
界強度の二乗に比例する。As shown in the equation, the thermal energy is proportional to the square of the electric field strength.
【００２０】以上説明したように、本発明者は、酵素免
疫反応測定用の被加熱試料のマイクロ波による加熱につ
いて鋭意研究を重ねた。その結果、マイクロ波を特定の
波長にすれば均一かつ迅速な加熱が可能となることを見
い出し、この知見に基づいて本発明をなすに至った。As described above, the present inventor has conducted extensive studies on heating of a sample to be heated for measuring enzyme immunoreaction by microwave. As a result, they have found that uniform and rapid heating is possible if the microwave has a specific wavelength, and the present invention has been completed based on this finding.
【００２１】すなわち、本発明に係る酵素免疫反応測定
用の温度撹拌装置は、マイクロ波を吸収することなく透
過させる物質から成る試料保持平板と、この試料保持平
板に一定間隔で多数設けられると共に酵素免疫反応測定
用の被加熱試料が入れられる有底穴と、前記試料保持平
板を収容する加熱室と、前記有底穴の設けられた一定間
隔の二倍以下の波長を有するマイクロ波を照射して前記
被加熱試料を加熱する加熱手段とを備えたものである。
また、前記有底穴の設けられた一定間隔が９mmであり、
前記加熱手段の照射するマイクロ波の波長が18mmである
ものとしてもよい。さらに、前記加熱手段によるマイク
ロ波照射時に前記試料保持平板を移動する試料保持平板
移動手段を付設したものとしてもよい。That is, the temperature agitation apparatus for measuring an enzyme immunoreaction according to the present invention comprises a sample holding plate made of a substance that allows microwaves to pass through without absorbing it, and a large number of enzyme are provided on the sample holding plate at regular intervals. A bottomed hole in which a heated sample for immune reaction measurement is placed, a heating chamber for accommodating the sample holding plate, and a microwave having a wavelength not more than twice the fixed interval provided in the bottomed hole are irradiated. And heating means for heating the sample to be heated.
Also, the fixed interval provided with the bottomed holes is 9 mm,
The microwave irradiated by the heating means may have a wavelength of 18 mm. Further, a sample holding plate moving means for moving the sample holding plate during microwave irradiation by the heating means may be additionally provided.
【００２２】[0022]
【作用】試料保持平板には、一定間隔で有底穴が多数設
けられている。これらの有底穴のそれぞれに、酵素免疫
反応測定用の被加熱試料を入れる。そして、試料保持平
板を温度撹拌装置の加熱室に収納する。続いて、加熱手
段からマイクロ波を試料保持平板に照射する。このマイ
クロ波の波長は、有底穴の設けられた一定間隔の二倍以
下の波長となっている。したがって、定在波化したマイ
クロ波による単位体積当たりの発熱量の空間分布の一周
期は、有底穴の設けられた一定間隔以下となる。すなわ
ち、隣接する被加熱試料において発熱量の差はほとんど
なく、各被加熱試料は均一に加熱される。また、被加熱
試料は、マイクロ波により内部から効率よく加熱され、
温度が迅速に上昇する。Function: The sample holding plate is provided with a number of bottomed holes at regular intervals. A heated sample for enzyme immunoassay measurement is placed in each of these bottomed holes. Then, the sample holding plate is housed in the heating chamber of the temperature stirring device. Then, the heating means irradiates the sample holding plate with microwaves. The wavelength of this microwave is not more than twice the fixed interval provided with the bottomed holes. Therefore, one period of the spatial distribution of the amount of heat generated per unit volume by the microwave that has been converted into the standing wave is equal to or less than the fixed interval in which the bottomed holes are provided. That is, there is almost no difference in the amount of heat generation between adjacent heated samples, and each heated sample is heated uniformly. Further, the sample to be heated is efficiently heated from the inside by the microwave,
The temperature rises quickly.
【００２３】[0023]
【実施例】図１は、本発明に係る温度撹拌装置の一実施
例を示す一部を切り欠いた正面図である。以下、この図
面に基づき説明する。DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 1 is a partially cutaway front view showing an embodiment of a temperature stirring device according to the present invention. Hereinafter, description will be given with reference to this drawing.
【００２４】本発明に係る温度撹拌装置１０は、マイク
ロ波Ｍを吸収することなく透過させる物質から成る試料
保持平板１２と、試料保持平板１２に一定間隔Ｄで多数
設けられると共に酵素免疫反応測定用の被加熱試料Ｓが
入れられる有底穴１４と、試料保持平板１２を収容する
加熱室１６と、有底穴１４の設けられた一定間隔Ｄの二
倍以下の波長を有するマイクロ波Ｍを照射して被加熱試
料Ｓを加熱する加熱手段１８とを備えたものである。The temperature agitation device 10 according to the present invention is provided with a sample holding plate 12 made of a substance that allows the microwave M to pass through without absorbing it, and a large number of the sample holding plate 12 are provided at regular intervals D and for measuring enzyme immunoreaction. Of the sample S to be heated, a heating chamber 16 for accommodating the sample-holding flat plate 12, and a microwave M having a wavelength equal to or less than twice the fixed interval D in which the bottomed holes 14 are provided. And heating means 18 for heating the sample S to be heated.
【００２５】マイクロ波Ｍを吸収することなく透過させ
る物質とは、式において誘電係数εｒ・ tanδが小さ
い物質をいい、例えば、ポリスチレン，ポリテトラフル
オロエチレン，石英等である。したがって、試料保持平
板１２は、マイクロ波Ｍによって実質的に加熱されな
い。The substance that transmits the microwave M without absorbing it means a substance having a small dielectric constant εr · tan δ in the formula, and examples thereof include polystyrene, polytetrafluoroethylene, and quartz. Therefore, the sample holding flat plate 12 is not substantially heated by the microwave M.
【００２６】加熱手段１８は、商用電源電圧を高電圧に
変換する電源トランス２０と、電源トランス２０で得ら
れた高電圧によってマイクロ波Ｍを発生させるマグネト
ロン２２と、マグネトロン２２で発生したマイクロ波Ｍ
を加熱室１６へ導くアンテナ２４及び導波管２６とから
構成されている。The heating means 18 includes a power transformer 20 for converting a commercial power source voltage into a high voltage, a magnetron 22 for generating a microwave M by the high voltage obtained by the power transformer 20, and a microwave M generated by the magnetron 22.
It is composed of an antenna 24 and a waveguide 26 that guide the light to the heating chamber 16.
【００２７】加熱室１６には、試料保持平板１２を載置
すると共に、必要に応じ移動する試料保持平板移動手段
３０が付設されている。試料保持平板移動手段３０は、
例えば、試料保持平板１２を載置したまま回転するいわ
ゆるターンテーブルや、試料保持平板１２を載置したま
まＸ方向又はＹ方向に往復移動するＸ−Ｙテーブル等で
ある。The heating chamber 16 is provided with a sample-holding plate 12 and a sample-holding plate moving means 30 for moving the sample-holding plate 12 as needed. The sample holding plate moving means 30 is
For example, a so-called turntable that rotates while mounting the sample holding flat plate 12 or an XY table that reciprocates in the X direction or the Y direction while mounting the sample holding flat plate 12 is used.
【００２８】本実施例では、一定間隔Ｄは９mm、マイク
ロ波Ｍは、波長が18mm，周波数が16.7GHz である。In this embodiment, the constant interval D is 9 mm, the microwave M has a wavelength of 18 mm and a frequency of 16.7 GHz.
【００２９】図２は、本実施例における被加熱試料の温
度分布とマイクロ波との関係を示す斜視図である。図３
は、本実施例における被加熱試料の温度分布の実測値を
示す三次元グラフである。以下、図１乃至図３に基づき
温度撹拌装置１０の動作を説明する。ただし、図４及び
図５と同一部分は同一符号を付して説明を省略する。FIG. 2 is a perspective view showing the relationship between the temperature distribution of the sample to be heated and the microwave in this embodiment. Figure 3
[Fig. 3] is a three-dimensional graph showing the actual measurement value of the temperature distribution of the sample to be heated in this example. The operation of the temperature stirring device 10 will be described below with reference to FIGS. 1 to 3. However, the same parts as those in FIG. 4 and FIG.
【００３０】試料保持平板１２には、一定間隔９mmで有
底穴１４が多数設けられている。これらの有底穴１４の
それぞれに、酵素免疫反応測定用の被加熱試料Ｓを入れ
る。そして、試料保持平板１２を加熱室１６に収納す
る。続いて、加熱手段１８からマイクロ波Ｍを試料保持
平板１２に照射する。このマイクロ波Ｍの波長は、有底
穴１４の設けられた一定間隔９mmの二倍の18mmとなって
いる。The sample-holding flat plate 12 is provided with a large number of bottomed holes 14 at regular intervals of 9 mm. A heated sample S for enzyme immunoassay measurement is placed in each of these bottomed holes 14. Then, the sample holding flat plate 12 is housed in the heating chamber 16. Then, the heating means 18 irradiates the sample holding flat plate 12 with the microwave M. The wavelength of the microwave M is 18 mm, which is twice the fixed interval 9 mm provided with the bottomed holes 14.
【００３１】図２において、波形ｍ１ｘ，ｍ１ｙは、そ
れぞれ波長が18mmのマイクロ波ＭのＸ方向成分，Ｙ方向
成分を示し、Ｚ方向を電界強度の絶対値としている。こ
れから明らかなように、定在波化したマイクロ波Ｍによ
る単位体積当たりの発熱量の空間分布の一周期は、有底
穴１４の設けられた一定間隔９mmにおおよそ一致する。
すなわち、隣接する被加熱試料Ｓにおいて発熱量の差は
ほとんどないので、各被加熱試料Ｓは均一に加熱され、
図３に示すような均一な温度分布が得られる。また、被
加熱試料Ｓは、マイクロ波Ｍにより内部から効率よく加
熱され、短時間で温度が上昇する。In FIG. 2, waveforms m1x and m1y respectively indicate the X-direction component and the Y-direction component of the microwave M having a wavelength of 18 mm, and the Z direction is the absolute value of the electric field strength. As is clear from this, one period of the spatial distribution of the amount of heat generated per unit volume by the microwave M that has been made into a standing wave approximately matches the fixed interval 9 mm in which the bottomed holes 14 are provided.
That is, since there is almost no difference in the calorific value between the adjacent samples S to be heated, each sample S to be heated is uniformly heated,
A uniform temperature distribution as shown in FIG. 3 is obtained. Further, the sample S to be heated is efficiently heated from the inside by the microwave M, and the temperature rises in a short time.
【００３２】また、図２及び図４において図示していな
いが、定在波化したマイクロ波の電界強度は、Ｚ方向に
も、Ｘ方向及びＹ方向と同様に分布している。したがっ
て、本実施例によれば、Ｚ方向にも一定間隔９mmにおい
て発熱量の差はほとんど生じない。そこで、試料保持平
板１２をＺ方向に複数枚保持して加熱室１６へ収容する
ことにより、一度に大量の被加熱試料Ｓを均一かつ短時
間に加熱できる。Although not shown in FIG. 2 and FIG. 4, the electric field strength of the microwave that has been converted into a standing wave is distributed in the Z direction as well as in the X and Y directions. Therefore, according to this embodiment, there is almost no difference in the amount of heat generated in the Z direction at a constant interval of 9 mm. Therefore, by holding a plurality of sample holding flat plates 12 in the Z direction and accommodating them in the heating chamber 16, a large amount of the sample S to be heated can be heated uniformly and in a short time.
【００３３】さらに、マイクロ波Ｍと試料保持平板移動
手段３０とを併用することによって、マイクロ波Ｍによ
って帯電した被加熱試料Ｓ内の分子を放電させることが
できるので、被加熱試料Ｓ内における目的外クーロン結
合（非特異的結合）を抑制することができる。Further, by using the microwave M and the sample holding plate moving means 30 together, the molecules in the heated sample S charged by the microwave M can be discharged, so that the purpose in the heated sample S can be improved. Outer Coulomb binding (non-specific binding) can be suppressed.
【００３４】なお、マイクロ波Ｍは水の吸収率の良好な
2.45GHz の整数倍とすると、2.45GHz と同様の吸収効率
が得られるので好ましい。The microwave M has a good water absorption rate.
An integral multiple of 2.45 GHz is preferable because the same absorption efficiency as 2.45 GHz can be obtained.
【００３５】[0035]
【発明の効果】本発明に係る酵素免疫反応測定用の温度
撹拌装置によれば、以下の効果を奏する。EFFECTS OF THE INVENTION The temperature stirring device for measuring enzyme immunoreaction according to the present invention has the following effects.
【００３６】試料保持平板に一定間隔で多数設けられて
た有底穴に、酵素免疫反応測定用の被加熱試料を入れ、
有底穴の設けられた一定間隔の二倍以下の波長のマイク
ロ波で各被加熱試料を加熱するようにしたので、隣接す
る被加熱試料において発熱量の差をほとんどなくすこと
ができ、被加熱試料全数をきわめて均一に加熱できる。
しかも、マイクロ波により被加熱試料を内部から効率よ
く加熱できるので、被加熱試料を短時間で所定の温度ま
で加熱することができる。A sample to be heated for enzyme immunoreaction measurement was placed in a bottomed hole provided in a large number at regular intervals on a sample holding plate,
Since each sample to be heated is heated with microwaves having a hole with a bottom and having a wavelength not more than twice the fixed interval, it is possible to almost eliminate the difference in the calorific value between adjacent samples to be heated. All samples can be heated very uniformly.
Moreover, since the sample to be heated can be efficiently heated from the inside by the microwave, the sample to be heated can be heated to a predetermined temperature in a short time.
【００３７】この場合、試料保持平板が収容される加熱
室内において、試料保持平板を積み重ねる方向に対して
も温度分布を均一にできるので、試料保持平板を複数枚
積み重ねることにより、一度に大量の被加熱試料を均一
かつ短時間に加熱できる。In this case, the temperature distribution can be made uniform even in the stacking direction of the sample holding flat plates in the heating chamber in which the sample holding flat plates are accommodated. The heated sample can be heated uniformly and in a short time.
【００３８】また、試料保持平板移動手段とを併用する
ことによって、マイクロ波によって帯電した被加熱試料
内の分子を放電させることができるので、被加熱試料内
における目的外クーロン結合を抑制することができる。Further, by using together with the sample holding plate moving means, it is possible to discharge the molecules charged in the sample to be heated by the microwave, so that it is possible to suppress unintended Coulomb bond in the sample to be heated. it can.
【図１】本発明に係る温度撹拌装置の一実施例を示す一
部を切り欠いた正面図である。FIG. 1 is a partially cutaway front view showing an embodiment of a temperature stirring device according to the present invention.
【図２】本実施例における被加熱試料の温度分布とマイ
クロ波との関係を示す斜視図である。FIG. 2 is a perspective view showing a relationship between a temperature distribution of a sample to be heated and a microwave in this example.
【図３】本実施例における被加熱試料の温度分布の実測
値を示す三次元グラフである。FIG. 3 is a three-dimensional graph showing measured values of the temperature distribution of the sample to be heated in this example.
【図４】従来例における被加熱試料の温度分布とマイク
ロ波との関係を示す斜視図である。FIG. 4 is a perspective view showing a relationship between a temperature distribution of a sample to be heated and a microwave in a conventional example.
【図５】従来例における被加熱試料の温度分布の実測値
を示す三次元グラフである。FIG. 5 is a three-dimensional graph showing the measured values of the temperature distribution of the sample to be heated in the conventional example.
１０ 温度撹拌装置 １２ 試料保持平板 １４ 有底穴 １６ 加熱室 １８ 加熱手段 ３０ 試料保持平板移動手段 Ｄ 有底穴の設けられた一定間隔 Ｍ マイクロ波 Ｓ 被加熱試料 10 Temperature Stirrer 12 Sample Holding Plate 14 Bottomed Hole 16 Heating Chamber 18 Heating Means 30 Sample Holding Plate Moving Means D Constant Interval with Bottomed Holes M Microwave S Sample to be Heated
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｈ０５Ｂ 6/64 Ｊ 6908−3Ｋ ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification number Office reference number FI technical display location H05B 6/64 J 6908-3K
Claims (3)
る物質から成る試料保持平板と、この試料保持平板に一
定間隔で多数設けられると共に酵素免疫反応測定用の被
加熱試料が入れられる有底穴と、前記試料保持平板を収
容する加熱室と、前記有底穴の設けられた一定間隔の二
倍以下の波長を有するマイクロ波を照射して前記被加熱
試料を加熱する加熱手段とを備えたことを特徴とする、
酵素免疫反応測定用の温度撹拌装置。1. A sample-holding plate made of a substance that allows microwaves to pass through without absorbing it, and a bottomed hole in which a large number of sample-holding plates are provided at regular intervals and into which a heated sample for enzyme immunoassay measurement is placed. A heating chamber for accommodating the sample-holding flat plate, and heating means for heating the sample to be heated by irradiating a microwave having a wavelength equal to or less than twice the fixed interval provided with the bottomed holes. Characterized by,
Temperature stirring device for measuring enzyme immunoreaction.
であり、前記加熱手段の照射するマイクロ波の波長が18
mmであることを特徴とする請求項１記載の酵素免疫反応
測定用の温度撹拌装置。2. The fixed interval provided with the bottomed holes is 9 mm.
And the wavelength of the microwave radiated by the heating means is 18
The temperature stirring device for enzyme immunoassay measurement according to claim 1, wherein the temperature stirring device is mm.
用の温度撹拌装置において、前記加熱手段によるマイク
ロ波照射時に前記試料保持平板を移動する試料保持平板
移動手段を付設したことを特徴とする酵素免疫反応測定
用の温度撹拌装置。3. The temperature stirring device for enzyme immunoassay measurement according to claim 1 or 2, further comprising sample holding plate moving means for moving the sample holding plate during microwave irradiation by the heating means. A temperature stirring device for measuring enzyme immunoreaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5351588A JPH07198572A (en) | 1993-12-28 | 1993-12-28 | Temperature agitator for enzyme immunoreaction assay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5351588A JPH07198572A (en) | 1993-12-28 | 1993-12-28 | Temperature agitator for enzyme immunoreaction assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07198572A true JPH07198572A (en) | 1995-08-01 |
Family
ID=18418290
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5351588A Withdrawn JPH07198572A (en) | 1993-12-28 | 1993-12-28 | Temperature agitator for enzyme immunoreaction assay |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07198572A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6686574B2 (en) * | 2002-06-29 | 2004-02-03 | Samsung Electronics Co., Ltd. | Microwave oven having two doors |
| WO2009075332A1 (en) | 2007-12-12 | 2009-06-18 | Saida Fds Inc. | Microwave applicator |
-
1993
- 1993-12-28 JP JP5351588A patent/JPH07198572A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6686574B2 (en) * | 2002-06-29 | 2004-02-03 | Samsung Electronics Co., Ltd. | Microwave oven having two doors |
| WO2009075332A1 (en) | 2007-12-12 | 2009-06-18 | Saida Fds Inc. | Microwave applicator |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
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