JPH0714400B2 - Adhesion formation method of periodontal tissue to implant - Google Patents
Adhesion formation method of periodontal tissue to implantInfo
- Publication number
- JPH0714400B2 JPH0714400B2 JP4187374A JP18737492A JPH0714400B2 JP H0714400 B2 JPH0714400 B2 JP H0714400B2 JP 4187374 A JP4187374 A JP 4187374A JP 18737492 A JP18737492 A JP 18737492A JP H0714400 B2 JPH0714400 B2 JP H0714400B2
- Authority
- JP
- Japan
- Prior art keywords
- periodontal ligament
- cells
- pseudo
- alveolar bone
- artificial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Description
【０００１】[0001]
【産業上の利用分野】本発明は天然の歯根膜類似の歯根
膜を有する人工歯根に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an artificial dental root having a periodontal ligament similar to natural periodontal ligament.
【０００２】[0002]
【従来の技術】歯が失われた症例に対して、従来は主と
して有床義歯や橋義歯などによる補綴処置がなされてき
た。しかしこのような処置では、咀嚼、発音機能や審美
性の復元が不十分で、患者に満足感を与えることのでき
ない難症例も少なくない。2. Description of the Related Art Conventionally, prosthodontic treatment using a bed denture or a bridge denture has been conventionally performed on a case where a tooth is lost. However, with such a treatment, there are many difficult cases in which the mastication, the pronunciation function, and the restoration of the aesthetics are insufficient and the patient cannot be satisfied.
【０００３】そこで最近このような症例を中心に、イン
プラント法が多用されるようになっている。このインプ
ラント法とはチタン、人工サファイア、ハイドロキシア
パタイトなどの生体に親和性のある材料からなる人工歯
根を歯槽骨または顎骨内に埋入し、これに人工歯や義歯
などの上部構造（人工歯冠）を保持して機能させる方法
である。Therefore, recently, the implant method has been widely used mainly in such cases. With this implant method, artificial tooth roots made of biocompatible materials such as titanium, artificial sapphire, and hydroxyapatite are embedded in the alveolar bone or jaw bone, and the upper structure (artificial tooth crown) ) Is a method of holding and functioning.
【０００４】インプラント法により補綴を行った例を図
１に示す。図中１２は歯肉、１４は歯槽骨、１５は人工
歯冠、１６は人工歯根（インプラント体）である。イン
プラント体１６と歯槽骨１４とは骨性癒着により直接接
着する。An example of performing prosthesis by the implant method is shown in FIG. In the figure, 12 is a gingiva, 14 is an alveolar bone, 15 is an artificial crown, and 16 is an artificial root (implant body). The implant body 16 and the alveolar bone 14 are directly adhered to each other by bony adhesion.
【０００５】インプラント法によれば歯肉から上の歯冠
部を天然の歯と同じ構造にできるため、義歯のような違
和感がなく、天然歯と同様の咀嚼、発音機能があり、美
観も損なわれない。According to the implant method, the crown above the gingiva can be made to have the same structure as a natural tooth, so that it does not have the discomfort of a denture, has the same chewing and pronunciation functions as a natural tooth, and impairs aesthetics. Absent.
【０００６】[0006]
【発明が解決しようとする課題】しかしインプラント法
では次のような欠点があった。すなわち人工歯根を歯槽
骨、または顎骨に直接埋め込むため、咀嚼時に歯に加わ
る外力が人工歯根を介して直接歯槽骨または顎骨に作用
する。一般に、骨の深層は海綿骨といわれる多孔な骨梁
から成り、空隙は骨髄で満たされている。このような骨
梁に対して咀嚼時の外力が衝撃的にあるいは繰り返し作
用すれば、生体反応としてその周辺に破骨細胞が誘導形
成され、骨吸収が急速に進行する。その結果インプラン
ト体の沈下、傾斜または移動などを生じ、最終的には人
工歯根の脱落につながる場合も多い。However, the implant method has the following drawbacks. That is, since the artificial root is directly embedded in the alveolar bone or the jawbone, the external force applied to the tooth during mastication directly acts on the alveolar bone or the jawbone via the artificial root. In general, the deep layer of bone consists of porous trabecular bone called cancellous bone, and the voids are filled with bone marrow. When an external force at the time of mastication is shocked or repeatedly acts on such a trabecular bone, osteoclasts are induced and formed around it as a biological reaction, and bone resorption rapidly progresses. As a result, the implant body may be submerged, tilted, or moved, and in many cases the artificial tooth root may eventually fall off.
【０００７】また歯垢中の細菌がインプラント体と歯槽
骨との接合面から侵入し歯槽骨の感染症を起こすことが
多く、そのため埋め込んだインプラント体を抜き取って
治療する必要が生じる。このように従来のインプラント
法によって補綴された人工歯は天然歯と同様の機能を長
期に保つことは困難であった。Bacteria in dental plaque often invade from the joint surface between the implant body and the alveolar bone to cause infection of the alveolar bone. Therefore, it is necessary to remove the embedded implant body for treatment. As described above, it has been difficult for the artificial tooth prostheses that have been prosthesized by the conventional implant method to maintain the same function as a natural tooth for a long period of time.
【０００８】そこで本発明の課題は咀嚼時に加わる外力
が歯槽骨に直接作用することを緩和し、細菌による感染
をできるだけ防止し、長期間天然歯と同様の機能を維持
するにはどうすればよいかという点にある。[0008] Therefore, the object of the present invention is to reduce the external force applied during mastication from directly acting on the alveolar bone, prevent infection by bacteria as much as possible, and maintain the same function as a natural tooth for a long time. In point.
【０００９】[0009]
【課題を解決するための手段】本発明ではこのような課
題を解決するために次に示す手段を採用した。すなわち
従来のインプラント法のようにインプラント体を直接歯
槽骨に定着するのではなくインプラント体と歯槽骨の間
に疑似歯根膜を介在させて定着するものである。 疑似
歯根膜の形成方法は次のようにする。まず歯の人工補綴
を行おうとする者、あるいは他の者から人歯根膜細胞を
採取し、それを培養する。培養された歯根膜細胞は一層
で横方向に広がるから、それを何層にもなるように重層
培養する。このようにして人の歯根膜組織に似た疑似歯
根膜を培養して形成する。In order to solve such a problem, the present invention adopts the following means. That is, the implant body is not fixed directly to the alveolar bone as in the conventional implant method, but is fixed by interposing a pseudo periodontal ligament between the implant body and the alveolar bone. The method of forming the pseudo periodontal ligament is as follows. First, human periodontal ligament cells are collected from a person who intends to perform a dental prosthesis or another person and cultured. Since the cultured periodontal ligament cells spread laterally in one layer, they are multi-layered in multiple layers. In this way, a pseudo periodontal ligament similar to human periodontal ligament is cultured and formed.
【００１０】[0010]
【作 用】天然の歯は図２に示すように歯根膜を介して
歯槽骨に接合し機能している。従来はこの歯根膜が咀嚼
時に歯槽骨に加わる衝撃的な力を緩衝する作用を有する
ことのみが強調されてきた。しかし歯根膜はその他に歯
垢沈着に伴なう細菌の侵入や歯周疾患の発症などに抵抗
していること、その周辺に固有歯槽骨を誘導し、周辺の
石灰化を促進して表層における石灰化度の高い歯槽骨を
形成するとともに、骨吸収に抵抗する破骨細胞分化抑制
因子を産出していること、あるいは歯根膜組織内に走行
する神経により咀嚼時に加わる力が感知され、咬合圧や
咀嚼リズムがコントロールされていることなどが最近明
らかになっている。[Working] As shown in Fig. 2, natural teeth function by connecting to the alveolar bone through the periodontal ligament. Conventionally, it has been emphasized that the periodontal ligament has a function of buffering an impacting force applied to the alveolar bone during mastication. However, the periodontal ligament also resists the invasion of bacteria associated with plaque deposition and the development of periodontal disease, induces alveolar bone in its periphery, promotes calcification in the periphery, and promotes calcification in the surface In addition to forming alveolar bone with a high degree of calcification, it is producing an osteoclast differentiation inhibitory factor that resists bone resorption, or the nerve running in the periodontal ligament senses the force applied during mastication, which causes occlusal pressure. Recently, it has become clear that the chewing rhythm is controlled.
【００１１】本発明はこの天然の歯根膜に似ている疑似
歯根膜をインプラント体と歯槽骨または顎骨との接合面
の間に介在させている。そして疑似歯根膜は原則として
人工補綴を行なう者の歯根膜細胞を採取してその者の血
清で培養して形成するため、これを歯槽骨内に移入して
も拒絶反応を起こすことなく生体の一部として付着す
る。このように疑似歯根膜が長期間存在するため、天然
の歯根膜と同様の効果が得られる。すなわちこの疑似歯
根膜が咀嚼時に歯槽骨に加わる外力を柔らげるクッショ
ンの役割を果たし、また口腔内から歯槽骨内への細菌の
侵入を防止する。そのため歯槽骨や顎骨が外力による破
壊、細菌の侵襲をうけることがなく、長期にわたって天
然の歯と同様の機能を保持できる。According to the present invention, the pseudo periodontal ligament, which resembles the natural periodontal ligament, is interposed between the joint surface of the implant body and the alveolar bone or the jaw bone. And, in principle, pseudo periodontal ligament is formed by collecting periodontal ligament cells of a person who performs artificial prosthesis and culturing them with the serum of the person. Attach as part. In this way, since the pseudo periodontal ligament exists for a long period of time, the same effect as that of natural periodontal ligament can be obtained. That is, this pseudo periodontal ligament plays the role of a cushion that softens the external force applied to the alveolar bone during mastication, and also prevents the invasion of bacteria from the oral cavity into the alveolar bone. Therefore, the alveolar bone and the jaw bone are not destroyed by external force and are not invaded by bacteria, and can retain the same function as a natural tooth for a long period of time.
【００１２】なお疑似歯根膜の基になる歯根膜細胞が補
綴処置を行なう者から採取できない場合でもＨＬＡ反応
などのマッチングテストを行い拒絶反応の少ない他人の
歯根膜細胞を使用すれば同様の効果が得られる。Even when the periodontal ligament cells, which are the basis of the pseudo periodontal ligament, cannot be collected from the person who performs the prosthesis treatment, the same effect can be obtained by using the matching test such as HLA reaction and using the periodontal ligament cells of other persons with less rejection. can get.
【００１３】[0013]
【実施例】本発明の一実施例について以下に説明する。
本発明はヒト歯根膜細胞を培養して疑似歯根膜を形成
し、その疑似歯根膜をインプラント体と歯槽骨の接合面
の間に介在させて人工歯根を歯槽骨または顎骨に定着す
る方法である。そこで以下（１）ヒト歯根膜細胞の培養
方法（２）重層培養法（３）人工歯根の定着方法に分け
て説明する。EXAMPLE An example of the present invention will be described below.
The present invention is a method of culturing human periodontal ligament cells to form a pseudo periodontal ligament and interposing the pseudo periodontal ligament between the implant body and the abutment surface of the alveolar bone to fix the artificial root to the alveolar bone or the jawbone. . Therefore, the following description will be divided into (1) a method for culturing human periodontal ligament cells, (2) a multilayer culture method, and (3) a method for fixing an artificial tooth root.
【００１４】（１） ヒト歯根膜細胞の培養方法 患者から得た抜去歯を直ちに生理食塩水にいれて氷冷す
る。次いで歯根中央部より歯根膜片を採取し、これを同
一患者血清（５〜２％）、抗真菌剤（フルコナゾールな
ど）および抗菌剤（ペニシリン、ストレプトマイシンな
ど）を添加したＭＥＭ（初代培養用液）に入れてコラー
ゲンコートシャーレで静置培養する。(1) Method for culturing human periodontal ligament cells Immediately put extracted teeth obtained from a patient into physiological saline and cool with ice. Then, a piece of periodontal ligament was collected from the central part of the tooth root, and MEM (primary culture liquid) containing the same patient serum (5 to 2%), antifungal agent (fluconazole, etc.) and antibacterial agent (penicillin, streptomycin, etc.) And culture statically in a collagen-coated petri dish.
【００１５】生長した細胞をトリプシン−ＥＤＴＡで分
散し、同一患者血清（５〜２％）を添加したＭＥＭ（増
殖用液）で継代培養し、約２×１０6個以上の細胞を得
る。次に患者血清（８〜１２％）を添加したＭＥＭにジ
メチルサルフォオキサイドを１０％またはグリセロール
を１５％添加して凍結保存液を調整する。The grown cells are dispersed with trypsin-EDTA and subcultured in MEM (proliferation solution) supplemented with the same patient serum (5-2%) to obtain about 2 × 10 6 cells or more. Next, a cryopreservation liquid is prepared by adding 10% of dimethyl sulfoxide or 15% of glycerol to MEM supplemented with patient serum (8-12%).
【００１６】それらの細胞を凍結保存液に混入し、チュ
ーブに入れてプログラミングフリーザーで凍結する。チ
ューブを液体窒素内に入れ細胞を凍結保存する。The cells are mixed in a cryopreservation solution, placed in a tube and frozen in a programming freezer. Place the tube in liquid nitrogen and cryopreserve the cells.
【００１７】（２） 重層培養法 （２−１） 歯根膜細胞液の形成 抜歯後の創傷あるいは歯槽骨の回復に合わせて、凍結保
存した歯根膜細胞を解凍し、再び同一患者血清を添加し
た増殖用液で培養する。(2) Layer-by-layer culture method (2-1) Formation of periodontal ligament cell fluid In accordance with the recovery of the wound or alveolar bone after tooth extraction, the cryopreserved periodontal ligament cells were thawed and the same patient serum was added again. Incubate with growth medium.
【００１８】 対数増殖期にある歯根膜細胞をトリプシ
ン−ＥＤＴＡ処理して分散し、いったんシングルセル状
態にする。このシングルセル状態の細胞を遠心法により
濃縮し増殖溶液を加え、４細胞／ｍｌ〜２×１０６細胞
／ｍｌ濃度とし細胞を増殖用液中に浮遊させる。この細
胞浮遊液と等量の０．２％コラーゲン培地を混和し、歯
根膜細胞液を得る。コラーゲン培地を加えるのは増殖用
液中での細胞の浮遊を継続させるためである。Periodontal ligament cells in the logarithmic growth phase are treated with trypsin-EDTA to disperse, and once brought into a single cell state. The cells in the single cell state are concentrated by a centrifugation method and a growth solution is added to make the cells have a concentration of 4 cells / ml to 2 × 10 6 cells / ml, and the cells are suspended in a growth solution. An equal amount of 0.2% collagen medium is mixed with this cell suspension to obtain periodontal ligament cell fluid. The reason why the collagen medium is added is to keep the cells suspended in the growth solution.
【００１９】（２−２） 重層培養法 このように形成された歯根膜細胞液を支持体に浸して培
養すると何層にも細胞が重なった重層の歯根膜細胞が得
られる。支持体は重層細胞が形成された後には不要とな
るので、後に消滅する物が望ましい。本実施例では人の
皮膚から生成するアテロコラーゲンスポンジ（これには
抗原性がないため人体に使用しても拒絶反応の心配はな
い）、あるいは蟹の甲羅から生成するキチンスポンジを
用いる。ただし支持体はこれに限る必要はない。次に重
層培養法を具体的に示す。(2-2) Layer-by-layer culturing method When the periodontal ligament cell liquid thus formed is immersed in a support and cultured, multi-layered periodontal ligament cells can be obtained. Since the support becomes unnecessary after the stratified cells are formed, it is desirable that the support disappears later. In this example, an atelocollagen sponge produced from human skin (which does not have an antigenicity and therefore does not cause a rejection reaction when used in the human body) or a chitin sponge produced from a crab shell. However, the support is not limited to this. Next, the multilayer culture method will be specifically described.
【００２０】培養用ペトリデッシュ内に支持体としてシ
ート状あるいは粒状のアテロコラーゲンスポンジまたは
キチンスポンジを静置し、歯根膜細胞液を静かに、そし
てスポンジが十分に浸るまで注入する。その後３７度Ｃ
Ｏ2インキュベーター（５％ＣＯ2の培養用恒温槽）内で
培養を継続する。コラーゲン培地のみで培養すると細胞
増殖に伴いコラーゲンの収縮が見られるが、キチンスポ
ンジまたはアテロコラーゲン・スポンジを用いると、こ
れが支持体となって形状が維持され、コラーゲン収縮に
よる不定形な変形を防ぐことができる。A sheet-like or granular atelocollagen sponge or chitin sponge is allowed to stand as a support in a culture petri dish, and periodontal ligament cell fluid is gently injected until the sponge is sufficiently immersed. Then 37 degrees C
Culturing is continued in an O 2 incubator (5% CO 2 culture constant temperature bath). When cultured in collagen medium alone, collagen contraction is seen with cell proliferation, but when chitin sponge or atelocollagen sponge is used, this serves as a support to maintain the shape and prevent irregular deformation due to collagen contraction. it can.
【００２１】増殖用液は１週間毎に半量づつ交換する。
４〜６週間の培養で重層培養は完成するが、このものは
生体内の歯根膜に比べ、膜の主要構成物である一型コラ
ーゲンの形成が未熟である。これを疑似歯根膜とする。The growth solution is replaced by half every week.
Although the multi-layered culture is completed after 4 to 6 weeks of culture, the type I collagen, which is the main constituent of the membrane, is less mature than the in vivo periodontal ligament. This is a pseudo periodontal ligament.
【００２２】（３） 人工歯根の定着方法 （３−１） 人工歯根面に疑似歯根膜を付着する方法 健全な歯が事故的要因で脱離した場合などこれを再び挿
入して固定すると健全な歯として復元する事が出来る。
このような歯の再植成功例においては脱離した歯の根面
に健全な歯根膜が生存していたため歯根膜から繊維状の
組織が歯槽骨内に侵入し歯が歯槽骨に定着したと考えら
れている。(3) Fixing method of artificial tooth root (3-1) Method of attaching pseudo periodontal ligament to artificial tooth root surface When a healthy tooth is detached due to an accidental factor, etc. Can be restored as a tooth.
In such a successful tooth replantation, a healthy periodontal ligament survived on the root surface of the detached tooth, so that fibrous tissue invaded into the alveolar bone from the periodontal ligament and the tooth settled in the alveolar bone. It is considered.
【００２３】そこで人工歯根と歯槽骨または顎骨の接合
面に疑似歯根膜を介在させる方法として第一に人工歯根
面に疑似歯根膜を付着させそれを歯槽骨または顎骨に人
工歯根が納まるように形成された穴に挿入する方法があ
る。人工歯根を穴に挿入したのち２、３カ月経過すれば
疑似歯根膜から繊維状の組織が歯槽骨または顎骨内に侵
入し人工歯根が定着する。人工歯根面に疑似歯根膜を付
着させる方法には次の二つがある。Therefore, as a method of interposing a pseudo periodontal ligament at the joint surface between the artificial tooth root and the alveolar bone or the jaw bone, first, a pseudo periodontal ligament is attached to the artificial tooth root surface so that the artificial tooth root fits in the alveolar bone or the jaw bone. There is a way to insert it into the hole. Two or three months after inserting the artificial tooth root into the hole, fibrous tissue penetrates into the alveolar bone or the jawbone from the pseudo periodontal ligament, and the artificial tooth root is fixed. There are the following two methods for attaching the pseudo periodontal ligament to the artificial tooth root surface.
【００２４】直接法 人工歯根面に直接歯根膜細胞を重層培養して疑似歯根膜
を付着させる方法である。Direct Method This is a method in which periodontal ligament cells are directly layer-cultured on the artificial tooth root surface to attach a pseudo periodontal ligament.
【００２５】人工歯根表面を細胞接着因子（セルタッ
ク、ヒト由来ラミニン、ヒト由来ビトロネクチンあるい
はヒト由来フィブロネクチン）で処理し、細胞吸着性を
高めてから人工歯根周囲にシート状の生体用有機材料
（アテロコラーゲンスポンジまたはキチンスポンジ）を
巻き付けて固定する。この面に０．１％コラーゲン培地
に浮遊させた歯根膜細胞液を付着させ、前述した（２）
の方法により表層に歯根膜細胞を重層培養して、疑似歯
根膜を形成する。この疑似歯根膜が付着した人工歯根を
歯槽骨または顎骨内の穴に差し込み定着する。The artificial tooth root surface is treated with a cell adhesion factor (CellTac, human-derived laminin, human-derived vitronectin or human-derived fibronectin) to enhance the cell adsorption property, and then a sheet-like biomaterial for organic matter (atelocollagen sponge) is provided around the artificial tooth root. Or wrap it around with a chitin sponge to fix it. To this surface, the periodontal ligament cell fluid suspended in 0.1% collagen medium was adhered, and the above-mentioned (2) was performed.
By culturing the periodontal ligament cells on the surface layer by the method described above, a pseudo periodontal ligament is formed. The artificial tooth root to which the pseudo periodontal ligament is attached is inserted into the hole in the alveolar bone or the jawbone and fixed.
【００２６】間接法 重層培養法により得た疑似歯根膜を、細胞接着因子でコ
ートした人工歯根面に巻き付けて固定し、同面と疑似歯
根膜の結合をさらに高めるために必要ならばさらに１週
間程度培養したのち、これを歯槽骨内の穴に差し込み定
着する。Indirect method The artificial periodontal ligament obtained by the overlay culture method is wound around and fixed on the artificial periodontal surface coated with the cell adhesion factor, and if necessary to further increase the bond between the artificial periodontal surface and the artificial periodontal ligament, a further week is required. After culturing to some extent, this is inserted into the hole in the alveolar bone and fixed.
【００２７】（３−２） 歯槽骨または顎骨の穴に疑似
歯根膜を付着する方法 人工歯根と歯槽骨の接合面に疑似歯根膜を介在させる第
二の方法として歯槽骨または顎骨に形成された穴に疑似
歯根膜を付着した後その穴に人工歯根を差し込み定着す
る方法がある。すなわち人工歯根を挿入する目的で骨内
に形成された穴に（２）の方法で形成した顆粒状疑似歯
根膜またはゲル状歯根膜細胞濃縮液を挿入したのち人工
歯根を差し込む方法である。(3-2) Method of attaching pseudo periodontal ligament to the hole of alveolar bone or jaw bone As a second method of interposing a pseudo periodontal ligament at the joint surface between the artificial tooth root and alveolar bone, it was formed in alveolar bone or jaw bone. There is a method of attaching a pseudo periodontal ligament to a hole and then inserting an artificial tooth root into the hole to fix it. That is, the artificial dental root is inserted after inserting the granular pseudo periodontal ligament or gel-like periodontal ligament cell concentrate formed by the method (2) into the hole formed in the bone for the purpose of inserting the artificial dental root.
【００２８】[0028]
【発明の効果】以上述べたように本発明による人工歯補
綴方法を用いれば、従来のインプラント法の構造が天然
歯に近いため違和感がないという長所を生かしながら、
その長期の使用に耐えないという欠点を防止できる。す
なわち本発明による方法を用いれば疑似歯根膜が咀嚼時
に歯槽骨に加わる外力を柔らげるクッションの役割を果
たし、また口腔内から歯槽骨内への細菌の侵入を防止す
る。そのため歯槽骨や顎骨が外力による破壊、細菌の侵
襲をうけることがなく、長期にわたって天然の歯と同様
の咀嚼機能、発音機能および美観を保持できる。本発明
はこのような耐久性のある人工歯を提供するため年齢に
関係なく優れた咀嚼機能の復元が可能となる。よく咀嚼
して物を食べられることは健康で幸福な生活にとって必
要不可欠のものであり、本発明は特に歯の欠損が多い中
高齢者に充実感を与えあるいは勤労意欲を高揚させる効
果がある。As described above, when the artificial tooth prosthesis method according to the present invention is used, the conventional implant method has a structure close to that of a natural tooth, so that there is no discomfort.
It is possible to prevent the drawback that it cannot withstand the long-term use. That is, when the method according to the present invention is used, the pseudo periodontal ligament plays the role of a cushion that softens the external force applied to the alveolar bone during mastication, and also prevents the invasion of bacteria from the oral cavity into the alveolar bone. Therefore, the alveolar bone and the jaw bone are not damaged by external force and are not invaded by bacteria, and the masticatory function, pronunciation function and aesthetics similar to those of natural teeth can be maintained for a long period of time. Since the present invention provides such a durable artificial tooth, excellent chewing function can be restored regardless of age. It is indispensable for a healthy and happy life to be able to chew well and eat food, and the present invention has the effect of giving a fulfilling feeling to the middle-aged and elderly people who have many tooth defects and enhancing their work motivation.
【図１】従来のインプラト法によって補綴された人工歯
および周辺部分を示す断面図である。FIG. 1 is a cross-sectional view showing an artificial tooth prosthesis and a peripheral portion prosthesis by a conventional implant method.
【図２】天然の歯とその周辺部分を示す断面図である。FIG. 2 is a cross-sectional view showing a natural tooth and its peripheral portion.
【図３】本発明の方法により補綴された人工歯およびそ
の周辺部分を示す断面図である。FIG. 3 is a cross-sectional view showing an artificial tooth prosthesis prosthesized by the method of the present invention and a peripheral portion thereof.
１０ 歯冠 １１ 歯根 １２ 歯肉 １３ 歯根膜 １４ 歯槽骨 １５ 人工歯冠 １６ 人工歯根（インプラント体） １７ 疑似歯根膜 10 dental crown 11 tooth root 12 gingiva 13 periodontal ligament 14 alveolar bone 15 artificial dental crown 16 artificial dental root (implant body) 17 pseudo periodontal ligament
Claims (2)
を培養して４細胞／ｍｌ〜２×１０ ６ 細胞／ｍｌ濃度と
し、これにコラーゲン培地を混和して歯根膜細胞液を
得、この歯根膜細胞液を生体用有機材料からなる支持体
に浸して培養し、疑似歯根膜を形成する重層培養法1. A human periodontal ligament cell collected from an extracted tooth is cultured to a concentration of 4 cells / ml to 2 × 10 6 cells / ml , and a collagen medium is mixed with this to obtain a periodontal ligament cell liquid, A layered culture method in which this periodontal ligament cell fluid is immersed in a support made of a biomaterial and cultured to form a pseudo periodontal ligament.
を培養し、培養された歯根膜細胞をさらに重層培養して
形成した疑似歯根膜を付着させた人工歯根 2. An artificial tooth root to which a pseudo periodontal ligament formed by culturing human periodontal ligament cells collected from extracted teeth and further culturing the cultured periodontal ligament cells
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4187374A JPH0714400B2 (en) | 1992-06-23 | 1992-06-23 | Adhesion formation method of periodontal tissue to implant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4187374A JPH0714400B2 (en) | 1992-06-23 | 1992-06-23 | Adhesion formation method of periodontal tissue to implant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH067381A JPH067381A (en) | 1994-01-18 |
| JPH0714400B2 true JPH0714400B2 (en) | 1995-02-22 |
Family
ID=16204894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4187374A Expired - Fee Related JPH0714400B2 (en) | 1992-06-23 | 1992-06-23 | Adhesion formation method of periodontal tissue to implant |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0714400B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8454362B2 (en) * | 2006-10-16 | 2013-06-04 | Natural Dental Implants Ag | Customized dental prosthesis for periodontal- or osseointegration, and related systems and methods |
| US10350030B2 (en) | 2006-10-16 | 2019-07-16 | Natural Dental Implants Ag | Methods of designing and manufacturing customized dental prosthesis for periodontal or osseointegration and related systems |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3471967B2 (en) * | 1995-03-28 | 2003-12-02 | カネボウ株式会社 | Hybrid artificial dental root |
| JP3657413B2 (en) * | 1997-12-02 | 2005-06-08 | 慶彦 清水 | Artificial tooth and manufacturing method thereof |
| JP3599701B2 (en) * | 2001-11-15 | 2004-12-08 | 独立行政法人理化学研究所 | Cell engraftment method on three-dimensional structure surface |
| JP5286484B2 (en) * | 2004-02-27 | 2013-09-11 | 国立大学法人広島大学 | Cryopreservation method and supply method of tooth extraction body |
| WO2013115128A1 (en) * | 2012-02-01 | 2013-08-08 | 株式会社オーガンテクノロジーズ | Dental implant and method for producing same |
-
1992
- 1992-06-23 JP JP4187374A patent/JPH0714400B2/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8454362B2 (en) * | 2006-10-16 | 2013-06-04 | Natural Dental Implants Ag | Customized dental prosthesis for periodontal- or osseointegration, and related systems and methods |
| US10350030B2 (en) | 2006-10-16 | 2019-07-16 | Natural Dental Implants Ag | Methods of designing and manufacturing customized dental prosthesis for periodontal or osseointegration and related systems |
| US11497583B2 (en) | 2006-10-16 | 2022-11-15 | Rtrs Investment, Llc | Methods of designing and manufacturing customized dental prosthesis for periodontal or osseointegration and related systems |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH067381A (en) | 1994-01-18 |
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