JPH07136507A - Endotoxin adsorbent - Google Patents
Endotoxin adsorbentInfo
- Publication number
- JPH07136507A JPH07136507A JP31104893A JP31104893A JPH07136507A JP H07136507 A JPH07136507 A JP H07136507A JP 31104893 A JP31104893 A JP 31104893A JP 31104893 A JP31104893 A JP 31104893A JP H07136507 A JPH07136507 A JP H07136507A
- Authority
- JP
- Japan
- Prior art keywords
- endotoxin
- paraffin
- composite material
- endotoxin adsorbent
- adsorbent according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- External Artificial Organs (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、新規な複合材からなる
エンドトキシン吸着材に関する。TECHNICAL FIELD The present invention relates to an endotoxin adsorbent comprising a novel composite material.
【0002】[0002]
【従来の技術】エンドトキシンは、リポ多糖であり代表
的な発熱性物質である。エンドトキシンにより汚染され
た血液、輸液、注射薬が体内に入ると発熱やショックな
どの副作用をひき起すため医薬品のエンドトキシンによ
る汚染はきびしく検査されている。そのため製薬用の
水、注射器の洗浄水、手術室で用いられる水などは、こ
れまでイオン交換水を蒸留し、さらに殺菌して密封され
ており、特別に薬品メーカーから購入して使用し、残っ
た水は廃棄するといった無駄とコストがかかるものであ
った。従って手軽にエンドトキシンフリーの水を得るこ
とができるシステムの開発が待たれているのが現状であ
る。2. Description of the Related Art Endotoxin is a lipopolysaccharide and is a typical pyrogenic substance. When blood, infusions, or injections contaminated with endotoxin enter the body, side effects such as fever and shock are caused, and thus endotoxin contamination of pharmaceuticals is strictly examined. Therefore, pharmaceutical water, water used to wash syringes, water used in operating rooms, etc., have been produced by distilling ion-exchanged water, sterilizing it, and sealing it. The water was wasteful and costly to dispose of. Therefore, at present, the development of a system that can easily obtain endotoxin-free water is awaited.
【0003】従来、エンドトキシンを除去する手段とし
ては、逆浸透膜や濾過膜を使用する方法、又は特開平1
−242105号公報に記載されているようなポリメチ
ルメタクリレート系樹脂の多孔質体による除去法が知ら
れている。しかしながら、これらの方法によっても、前
者は血液等には適用できず、又、後者は、タンパク質中
のエンドトキシン除去はできないという問題点がある。Conventionally, as a means for removing endotoxin, a method using a reverse osmosis membrane or a filtration membrane, or Japanese Patent Laid-Open No.
There is known a method for removing a polymethylmethacrylate resin with a porous body as described in Japanese Patent Publication No. 242105. However, even by these methods, the former cannot be applied to blood and the like, and the latter cannot remove endotoxin in a protein.
【0004】[0004]
【発明が解決しようとする課題】本発明の目的は、エン
ドトキシンの吸着性能にすぐれた複合材を提供すること
によってエンドトキシンフリーの水を効率よく低コスト
で製造することを可能にし、又、タンパク質中のエンド
トキシンを低コストで除去することを可能にしようとす
るものである。An object of the present invention is to provide a composite material having excellent endotoxin adsorption performance, thereby making it possible to efficiently produce endotoxin-free water at low cost, and It is intended to enable low-cost removal of endotoxins of.
【0005】[0005]
【課題を解決するための手段】本発明は、次式で示され
るパラフィンオイル又はワックスを有機溶剤に分散又は
溶解させ、適度の粒径を有する種々の無機物に付着させ
た複合材からなるエンドトキシン吸着材にある。DISCLOSURE OF THE INVENTION The present invention is an endotoxin adsorption comprising a composite material in which a paraffin oil or wax represented by the following formula is dispersed or dissolved in an organic solvent and adhered to various inorganic substances having an appropriate particle diameter. It is in wood.
【0006】以下、本発明を具体的に説明する。本発明
で用いられるパラフィンは、次の一般式で表わされるパ
ラフィンオイル又はワックスである。The present invention will be specifically described below. The paraffin used in the present invention is a paraffin oil or wax represented by the following general formula.
【0007】[0007]
【化2】 [Chemical 2]
【0008】上記一般式で示したパラフィンは、式中R
1 ,R2 ,R3 がメチル基、エチル基、プロピル基、ブ
チル基、ヘプチル基、フェニル基何れでもよいが、好ま
しいのはメチル基である。The paraffin represented by the above general formula is R in the formula.
1 , R 2 and R 3 may be any of a methyl group, an ethyl group, a propyl group, a butyl group, a heptyl group and a phenyl group, but a methyl group is preferable.
【0009】本発明で用いられるパラフィンは、パラフ
ィンオイル又はパラフィンワックスであって分子量25
0以上のものが好ましい。The paraffin used in the present invention is paraffin oil or paraffin wax having a molecular weight of 25.
Those of 0 or more are preferable.
【0010】次に、本発明で用いられる無機物は、平均
粒径が5〜500μmの範囲のものであれば何れでもよ
いが、好ましくは20〜300μmのものである。この
ような無機物の例としては、シリカゲル、アルミナ、酸
化チタン、水酸化アルミニウム、タルク等を挙げること
ができる。これらの中シリカゲル、アルミナの使用が好
ましい。これら無機物は、単独で使用するか又は混合し
て用いてもよい。The inorganic material used in the present invention may be any one having an average particle size of 5 to 500 μm, preferably 20 to 300 μm. Examples of such inorganic materials include silica gel, alumina, titanium oxide, aluminum hydroxide, talc and the like. Of these, silica gel and alumina are preferably used. These inorganic substances may be used alone or as a mixture.
【0011】本発明の複合材は、パラフィンをエチルア
ルコールに分散させるか又はハロゲン化炭化水素、ヘキ
サン等の有機溶剤に溶解して無機物に混合、付着、乾燥
することによって得られる。The composite material of the present invention can be obtained by dispersing paraffin in ethyl alcohol or by dissolving it in an organic solvent such as a halogenated hydrocarbon or hexane, mixing it with an inorganic substance, adhering it, and drying it.
【0012】本発明のエンドトキシン吸着材であるパラ
フィンと無機物との複合材中の両成分の混合比は、パラ
フィン/無機物=0.05〜50/99.95〜50重
量比部、好ましくは0.1〜30/99.9〜70重量
比である。パラフィンが0.05重量%未満であると吸
着能が充分でなく、50重量%を超えると複合材の取り
扱いが困難になり、本発明の目的にそぐわない。The mixing ratio of both components in the composite material of the endotoxin adsorbent of the present invention, paraffin and inorganic material, is such that paraffin / inorganic material = 0.05 to 50 / 99.95 to 50 parts by weight, preferably 0. 1 to 30 / 99.9 to 70 weight ratio. If the amount of paraffin is less than 0.05% by weight, the adsorptivity is not sufficient, and if it exceeds 50% by weight, the composite material becomes difficult to handle, which is not suitable for the purpose of the present invention.
【0013】[0013]
【実施例】以下、実施例によって本発明を具体的に説明
する。エンドトキシン吸着量(ng/gパラフィン)
(以下、ng/gパラフィンと略記する)は、平均粒径
50μmのシリカゲルの表面にパラフィン又はワックス
をコーティングし、これをカラムクロマト用ガラス管に
つめ、1000〜2000pgのエンドトキシンを含有
する水を通水し、一定時間毎にエンドトキシン量をカラ
ム入口、出口の水について求め、総吸着量を求めた。エ
ンドトキシン量は、和光純薬(株)のリムルスHSJ−
IIテストワコーを用い、比濁法により定量した。EXAMPLES The present invention will be specifically described below with reference to examples. Adsorption amount of endotoxin (ng / g paraffin)
(Hereinafter, abbreviated as ng / g paraffin) is a silica gel having an average particle size of 50 μm coated with paraffin or wax, which is filled in a glass tube for column chromatography and passed through water containing 1000 to 2000 pg of endotoxin. After watering, the amount of endotoxin was determined for the water at the column inlet and outlet at regular time intervals, and the total adsorption amount was determined. The amount of endotoxin is Limulus HSJ- manufactured by Wako Pure Chemical Industries, Ltd.
It was quantified by the turbidimetric method using II Test Wako.
【0014】[実施例1]流動パラフィン2gをヘキサ
ンに溶解し、平均粒径50μmのシリカゲル100gと
混合し、セパラブルフラスコ中で撹拌しながら60℃に
加温してヘキサンを除き乾燥した。乾燥後のシリカゲル
複合材20gを直径2cmのガラス製のクロマト管につ
め、1000〜2000pgのエンドトキシンを含む水
を通水して総吸着量を求めたところ6μg/gパラフィ
ンであった。Example 1 2 g of liquid paraffin was dissolved in hexane, mixed with 100 g of silica gel having an average particle size of 50 μm, heated to 60 ° C. in a separable flask with stirring to remove hexane, and dried. 20 g of the dried silica gel composite material was packed in a glass chromatography tube having a diameter of 2 cm, and water containing 1000 to 2000 pg of endotoxin was passed through to obtain a total adsorption amount of 6 μg / g paraffin.
【0015】[実施例2]実施例1と同様にして、ただ
し、流動パラフィンとシリカゲルの混合比を表1のよう
に変更して実験を行った。結果を表1に示した。複合材
中の流動パラフィンの量が0.05重量%以下のものは
総吸着量が少なく、20重量%を超えると飽和すること
がわかった。Example 2 An experiment was conducted in the same manner as in Example 1, except that the mixing ratio of liquid paraffin and silica gel was changed as shown in Table 1. The results are shown in Table 1. It was found that when the amount of liquid paraffin in the composite material was 0.05% by weight or less, the total amount of adsorption was small, and when it exceeded 20% by weight, it was saturated.
【0016】[0016]
【表1】 [Table 1]
【0017】[実施例3]実施例1と同様にして、ただ
し、無機物の種類を表2のように変えて行った。結果を
表2にまとめて示した。何れも同レベルの吸着能を示し
た。[Example 3] The same procedure as in Example 1 was carried out, except that the kinds of inorganic substances were changed as shown in Table 2. The results are summarized in Table 2. All showed the same level of adsorption capacity.
【0018】[0018]
【表2】 [Table 2]
【0019】[実施例4]実施例1と同様にして、ただ
し、種々の融点のパラフィンを用いて行った。結果を表
3にまとめて示した。分子量150以下のものは乾燥中
に蒸発してしまい使いものにならなかった。Example 4 The procedure of Example 1 was repeated, except that paraffins having various melting points were used. The results are summarized in Table 3. Those having a molecular weight of 150 or less were not usable because they were evaporated during drying.
【0020】[0020]
【表3】 [Table 3]
【0021】[実施例5]タンパク質として牛血清アル
ブミン(5wt/vol%)水溶液(pH=4.0)、
コラーゲン0.3wt%/vol水溶液(pH3.0)
を通水して、除去能について調べた。タンパク中のエン
ドトキシン量は、牛血清アルブミン200ng/ml、
コラーゲン50ng/ml含まれていた。0.1gのパ
ラフィン量でアルブミンについては30ml、コラーゲ
ンについては120mlまで除去できることがわかっ
た。タンパク質は98%以上回収された。結果を表4に
示した。[Example 5] A bovine serum albumin (5 wt / vol%) aqueous solution (pH = 4.0) as a protein,
Collagen 0.3 wt% / vol aqueous solution (pH 3.0)
After passing water, the removal ability was examined. The amount of endotoxin in the protein was 200 ng / ml of bovine serum albumin,
The collagen contained 50 ng / ml. It has been found that a paraffin amount of 0.1 g can remove up to 30 ml of albumin and up to 120 ml of collagen. More than 98% of the protein was recovered. The results are shown in Table 4.
【0022】[0022]
【表4】 [Table 4]
Claims (5)
溶剤に分散又は溶解させ、無機物に付着した複合材から
なることを特徴とするエンドトキシン吸着材。1. An endotoxin adsorbent characterized by comprising a composite material in which paraffin having a molecular weight of 250 or more is dispersed or dissolved in an organic solvent and attached to an inorganic substance.
物である請求項1のエンドトキシン吸着材。 【化1】 2. The endotoxin adsorbent according to claim 1, wherein the paraffin is a compound having a main structure represented by the following formula. [Chemical 1]
る請求項1又は2のエンドトキシン吸着材。3. The endotoxin adsorbent according to claim 1, wherein the inorganic particles have an average particle size of 5 to 500 μm.
0.05〜50重量%である請求項1、2又は3のエン
ドトキシン吸着材。4. The endotoxin adsorbent according to claim 1, 2 or 3, wherein the adhering ratio of paraffin to the inorganic substance is 0.05 to 50% by weight.
タン、水酸化アルミニウム、タルクの少なくとも1種で
ある請求項3のエンドトキシン吸着材。5. The endotoxin adsorbent according to claim 3, wherein the inorganic substance is at least one of silica gel, alumina, titanium oxide, aluminum hydroxide and talc.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP31104893A JPH07136507A (en) | 1993-11-18 | 1993-11-18 | Endotoxin adsorbent |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP31104893A JPH07136507A (en) | 1993-11-18 | 1993-11-18 | Endotoxin adsorbent |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH07136507A true JPH07136507A (en) | 1995-05-30 |
Family
ID=18012486
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP31104893A Pending JPH07136507A (en) | 1993-11-18 | 1993-11-18 | Endotoxin adsorbent |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH07136507A (en) |
-
1993
- 1993-11-18 JP JP31104893A patent/JPH07136507A/en active Pending
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