JPH07135958A - Agitated culture tank - Google Patents
Agitated culture tankInfo
- Publication number
- JPH07135958A JPH07135958A JP31260993A JP31260993A JPH07135958A JP H07135958 A JPH07135958 A JP H07135958A JP 31260993 A JP31260993 A JP 31260993A JP 31260993 A JP31260993 A JP 31260993A JP H07135958 A JPH07135958 A JP H07135958A
- Authority
- JP
- Japan
- Prior art keywords
- culture tank
- stirring
- culture
- baffle plate
- wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【0001】[0001]
【産業上の利用分野】本発明は、撹拌培養槽に関する。
更に詳しくは、微生物による物質生産の高効率化を達成
させる撹拌培養槽に関する。TECHNICAL FIELD The present invention relates to a stirred culture tank.
More specifically, the present invention relates to a stirred culture tank that achieves high efficiency of substance production by microorganisms.
【0002】[0002]
【従来の技術】邪魔板を培養槽内壁面に直接取り付けた
撹拌培養槽を用いて、微生物による物質生産が一般に行
われているが、その際撹拌翼の回転数を増加させると、
基質との接触回数の増加、微生物周辺の拡散層の減少な
どから、生産される物質量が増加することになる。2. Description of the Related Art A substance is generally produced by microorganisms by using a stirring culture tank having a baffle plate directly attached to the inner wall surface of the culture tank. At that time, if the rotation speed of the stirring blade is increased,
The amount of substances produced will increase due to the increase in the number of contact with the substrate and the decrease in the diffusion layer around the microorganisms.
【0003】しかしながら、実際には撹拌翼による微生
物の剪断、撹拌回転数の増加に伴う培養液中の気泡、特
に気泡径の増大などにより、ある撹拌回転数を境に物質
生産量はかえって減少するようになる。また、培養槽内
に設置された邪魔板により発生する渦によっても、物質
生産量は大きく左右される。However, in reality, due to shearing of microorganisms by the stirring blades, bubbles in the culture broth with an increase in the stirring rotation number, especially an increase in the bubble diameter, the substance production amount rather decreases at a certain stirring rotation number. Like In addition, the substance production amount is greatly influenced by the vortex generated by the baffle plate installed in the culture tank.
【0004】[0004]
【発明が解決しようとする課題】本発明の目的は、一定
以上の撹拌回転数で撹拌した場合においても、微生物に
よって生産される物質量を減少させないばかりか、かえ
って増加させる撹拌培養槽を提供することにある。DISCLOSURE OF THE INVENTION An object of the present invention is to provide a stirring culture tank which not only reduces the amount of substances produced by microorganisms but rather increases the amount even when agitating at a stirring speed of a certain level or more. Especially.
【0005】[0005]
【課題を解決するための手段】かかる本発明の目的は、
邪魔板を設置した撹拌培養槽において、邪魔板を培養槽
内壁面から隔離した位置に設置した撹拌培養槽によって
達成される。The object of the present invention is as follows.
In a stirring culture tank having a baffle plate, this is achieved by a stirring culture tank having the baffle plate installed at a position separated from the inner wall surface of the culture tank.
【0006】培養槽としては、円形のもの、角形のもの
など任意のものを使用することができるが、一般には円
形の培養槽が用いられる。その円形培養槽内には、1枚
またはそれ以上、一般には2〜8枚程度の邪魔板が円形培
養槽の半径方向に向けて設置され、その際培養槽内壁面
と邪魔板端面との間が、円形培養槽の大きさにもよる
が、一般には約0.5〜5cm程度、好ましくは約0.5〜2cm程
度隔離されるように設置される。また、邪魔板の形状
は、長方形、直角三角形など、撹拌効果が損なわれない
限り、任意の形状をとり得る。As the culture tank, any one such as a circular one and a rectangular one can be used, but a circular culture tank is generally used. Inside the circular culture tank, one or more baffle plates, generally about 2 to 8 baffles, are installed in the radial direction of the circular culture tank, and at this time, between the inner wall surface of the culture tank and the end face of the baffle plate. However, depending on the size of the circular culture tank, they are generally installed so as to be separated by about 0.5 to 5 cm, preferably about 0.5 to 2 cm. The baffle plate may have any shape, such as a rectangle or a right triangle, as long as the stirring effect is not impaired.
【0007】かかる培養槽において、撹拌翼は一般に邪
魔板下部よりも低い位置に設置され、邪魔板上部よりも
低い位置に液面を有する培養液を撹拌しながら、各種微
生物による培養を行わしめる。In such a culture tank, the stirring blades are generally installed at a position lower than the lower part of the baffle plate, and culture with various microorganisms is carried out while stirring the culture solution having a liquid surface at a position lower than the upper part of the baffle plate.
【0008】[0008]
【発明の効果】撹拌培養槽の邪魔板を培養槽内壁面から
隔離した位置に設置することにより、撹拌翼によって撹
拌された培養液中の気泡径は小さくなり、渦の発生を大
きくすることができ、その結果邪魔板を培養槽内壁面に
直接取り付けた培養槽を用いた場合には、一定以上の撹
拌回転数で撹拌すると微生物による生産物質量が急激に
減少するのとは全く対称的に、生産物質量の急激な増加
効果がみられるようになる。By installing the baffle plate of the stirring culture tank at a position separated from the inner wall surface of the culture tank, the bubble diameter in the culture solution stirred by the stirring blade can be reduced and the generation of vortices can be increased. As a result, in the case of using a culture tank with a baffle plate directly attached to the inner wall surface of the culture tank, it is quite symmetrical with the sudden decrease in the amount of substances produced by microorganisms when the stirring speed is higher than a certain value. , The effect of increasing the amount of produced substances will be seen.
【0009】[0009]
【実施例】次に、実施例について本発明を説明する。EXAMPLES The present invention will now be described with reference to examples.
【0010】実施例 下記構造の培養槽を用い、L-broth(トリプトン1%、酵母
エキス0.5%、NaCl 0.5%;殺菌前のpH7.0)にトリプトフ
ァン3gを加えた培地でのEnterbacter Cloacae No.11-5
(FERM P-8884)によるインドール酢酸の生産(特開昭63-4
2693号公報参照)速度を測定した。Example Using an incubator having the following structure, Enterbacter Cloacae No. 11-5
Production of indole acetic acid by (FERM P-8884) (Japanese Patent Laid-Open No. 63-4
The speed was measured.
【0011】直径10.5cmの培養槽内には、6cmの初期液
面で培養液が仕込まれており、幅2.5cm、厚さ0.5cmの逆
L字形邪魔板が長さ10cmの内の1.5cmの部分が培養液中
に浸漬されるように、培養槽内壁面から1.5cm隔離して
設置した。そして、培養槽底面から1.5cmの位置に、直
径8.0cmの撹拌翼を設置した。A culture solution having a diameter of 10.5 cm is filled with a culture solution having an initial liquid level of 6 cm, and an inverted L-shaped baffle plate having a width of 2.5 cm and a thickness of 0.5 cm is 1.5 cm out of a length of 10 cm. It was placed 1.5 cm away from the inner wall surface of the culture tank so that the part of was immersed in the culture solution. Then, a stirring blade having a diameter of 8.0 cm was installed at a position 1.5 cm from the bottom of the culture tank.
【0012】このような状態で、撹拌回転数200rpmまた
は300rpmで撹拌翼を回転させたときの液面深さ(撹拌前
の初期液面と撹拌した場合の液面との差)は、それぞれ
1.1cmおよび1.5cmであった。また、気泡径(0.1%の界面
活性剤を含む500mlの水溶液を仕込んで撹拌し、気泡を
発生させた後撹拌を止め、液面近くに上昇してきた気泡
の直径を写真撮影して平均した)は、0.30mmであった。In such a state, the liquid level depth (difference between the initial liquid level before stirring and the liquid level when stirring) when the stirring blade was rotated at 200 rpm or 300 rpm was
It was 1.1 cm and 1.5 cm. In addition, the bubble diameter (500 ml of an aqueous solution containing 0.1% surfactant was charged and stirred, after generating the bubbles, the stirring was stopped, and the diameter of the bubbles rising near the liquid surface was photographed and averaged) Was 0.30 mm.
【0013】更に、インドール酢酸生産速度(撹拌翼を
撹拌回転数750rpmで回転させ、37℃で培養した後、0.1M
NaH2PO4、15%アセトニトリル、pH3.5を展開溶媒とし
て、カラム:東ソー製ODS. 80TM、カラム温度:35℃、
流速:2ml/分、波長:254nmの条件下で測定したHPLCに
よるインドール酢酸濃度を培養日数で割った値)は、41
μg/ml/dayであった。Further, the indole acetic acid production rate (the stirring blade was rotated at a stirring speed of 750 rpm, and the mixture was incubated at 37 ° C.
Using NaH 2 PO 4 , 15% acetonitrile, pH 3.5 as developing solvent, column: Tosoh ODS.80TM, column temperature: 35 ° C,
The value obtained by dividing the concentration of indole acetic acid by HPLC measured by the conditions of flow rate: 2 ml / min and wavelength: 254 nm by the number of culture days) was 41.
It was μg / ml / day.
【0014】比較例 従来型の培養槽を用い、幅2.5cm、厚さ0.5cmの邪魔板を
培養槽内壁面に直接接するように設置したとき、撹拌回
転数200rpmまたは300rpmで撹拌翼を回転させたときの液
面深さはそれぞれ0.5cmおよび1.1cmで、気泡径は0.28mm
であった。また、インドール酢酸生産速度は、6.5μg/m
l/dayであった。Comparative Example When a conventional culture tank was used and a baffle plate having a width of 2.5 cm and a thickness of 0.5 cm was installed in direct contact with the inner wall surface of the culture tank, the stirring blade was rotated at 200 rpm or 300 rpm. The liquid surface depth is 0.5 cm and 1.1 cm, respectively, and the bubble diameter is 0.28 mm.
Met. Indole acetic acid production rate is 6.5 μg / m
It was l / day.
【0015】これらの実施例および比較例で撹拌回転数
を種々変更したときのインドール酢酸(IAA)生産速度は
図1に示されており、一定以上の撹拌回転数で撹拌した
とき、微生物によって生産される物質量がかえって増加
することが分かる。The indole acetic acid (IAA) production rate when the stirring speed was variously changed in these Examples and Comparative Examples is shown in FIG. 1, and was produced by the microorganism when stirring at a stirring speed above a certain level. It can be seen that the amount of substance that is consumed increases.
【図1】実施例および比較例における撹拌回転数とIAA
生産速度との関係を示すグラフである。FIG. 1 Stirring speed and IAA in Examples and Comparative Examples
It is a graph which shows the relationship with a production rate.
Claims (1)
邪魔板を培養槽内壁面から隔離した位置に設置してなる
撹拌培養槽。1. A stirring culture tank equipped with a baffle plate,
A stirring culture tank in which a baffle plate is installed at a position separated from the inner wall surface of the culture tank.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP31260993A JPH07135958A (en) | 1993-11-18 | 1993-11-18 | Agitated culture tank |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP31260993A JPH07135958A (en) | 1993-11-18 | 1993-11-18 | Agitated culture tank |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH07135958A true JPH07135958A (en) | 1995-05-30 |
Family
ID=18031271
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP31260993A Pending JPH07135958A (en) | 1993-11-18 | 1993-11-18 | Agitated culture tank |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH07135958A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017013974A1 (en) * | 2015-07-22 | 2017-01-26 | 株式会社日立製作所 | Stirring device |
-
1993
- 1993-11-18 JP JP31260993A patent/JPH07135958A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017013974A1 (en) * | 2015-07-22 | 2017-01-26 | 株式会社日立製作所 | Stirring device |
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