JPH07123976A - Method of cleaning microbe and water or soil - Google Patents
Method of cleaning microbe and water or soilInfo
- Publication number
- JPH07123976A JPH07123976A JP5273877A JP27387793A JPH07123976A JP H07123976 A JPH07123976 A JP H07123976A JP 5273877 A JP5273877 A JP 5273877A JP 27387793 A JP27387793 A JP 27387793A JP H07123976 A JPH07123976 A JP H07123976A
- Authority
- JP
- Japan
- Prior art keywords
- soil
- trichloroethylene
- trichlorethylene
- water
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、トリクロロエチレンを
効率よく分解する微生物およびそれを用いた水または土
壌の浄化方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism that decomposes trichlorethylene efficiently and a method for purifying water or soil using the microorganism.
【０００２】[0002]
【従来の技術】周知のように、先端産業において洗浄剤
等として使用されるトリクロロエチレンによる地下水や
土壌の汚染が指摘されており、発癌性の疑いのあるトリ
クロロエチレンの処理方法が早急に求められている。As is well known, it has been pointed out that groundwater and soil are contaminated with trichlorethylene used as a cleaning agent and the like in advanced industries, and a treatment method for trichlorethylene suspected of being carcinogenic is urgently required. .
【０００３】これまで、トリクロロエチレンに汚染され
た地下水の処理方法として、真空吸引法やエアーストリ
ッピンング法などの物理的処理を行うことによりトリク
ロロエチレンを分離する方法が提案されている。As a method of treating groundwater contaminated with trichlorethylene, there has been proposed a method of separating trichlorethylene by a physical treatment such as a vacuum suction method or an air stripping method.
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、これら
の方法では、大気汚染防止等の二次汚染を防止する観点
から、活性炭処理等の二次処理を必要とするが、係る処
理を施しても、完全には除去できないのが現状である。However, these methods require a secondary treatment such as activated carbon treatment from the viewpoint of preventing secondary pollution such as prevention of air pollution. However, even if such treatment is performed, At present, it cannot be completely removed.
【０００５】一方、近年、トリクロロエチレンを微生物
によって極めて効率よく分解し無害化するいわゆる生物
浄化法に関する研究が進められている。この生物浄化法
は、微生物の分解機能を用いるため、上記の物理的処理
方法に比べて多大なエネルギーを必要とせず、二次汚染
を招来することなく、しかも、原位置での広範囲にわた
った処理が可能である等のバイオ技術の利点を備えた優
れた方法である。On the other hand, in recent years, studies have been conducted on a so-called biological purification method in which trichlorethylene is decomposed by microorganisms extremely efficiently to render it harmless. Since this biological purification method uses the function of degrading microorganisms, it does not require much energy compared to the above physical treatment methods, does not cause secondary pollution, and spreads over a wide range in situ. It is an excellent method with the advantages of biotechnology such as being processable.
【０００６】本発明は、上記の観点よりなされたもので
あり、トリクロロエチレンを効率よく分解できる微生物
を提供することを目的としている。また、本発明は、ト
リクロロエチレンに汚染された水または土壌を二次汚染
を招来することなく、効率よく浄化することができる水
または土壌の浄化方法を提供することを目的としてい
る。The present invention has been made from the above viewpoint, and an object thereof is to provide a microorganism capable of decomposing trichlorethylene efficiently. Another object of the present invention is to provide a method for purifying water or soil that can efficiently purify water or soil contaminated with trichlorethylene without causing secondary pollution.
【０００７】[0007]
【課題を解決するための手段】本発明に係る微生物は、
アルカリジェネス エウロトロフス（Alcaligenes eurt
rophus）に属し、トリクロロエチレン分解能を有するこ
とを特徴とするものである。The microorganism according to the present invention comprises:
Alcaligenes eurt
rophus) and has the ability to decompose trichlorethylene.
【０００８】また、本発明に係る水または土壌の浄化方
法は、請求項１若しくは請求項２に記載の微生物をトリ
クロロエチレンに汚染された水または土壌に接種・混合
してこれら水または土壌に含まれたトリクロロエチレン
を分解処理する水または土壌の浄化方法に係り、前記水
または土壌に前記微生物を接種・混合するに際して、少
なくとも一種以上の芳香族化合物を添加混合することを
特徴としている。Further, the method for purifying water or soil according to the present invention comprises inoculating and mixing the microorganisms according to claim 1 or 2 into water or soil contaminated with trichlorethylene to be contained in these water or soil. In addition, the present invention relates to a method for decomposing trichloroethylene for water or soil, and is characterized in that at least one or more aromatic compounds are added and mixed when inoculating and mixing the water or soil with the microorganisms.
【０００９】[0009]
【作用】本発明に係る微生物は、芳香族化合物の存在
下、水や土壌中において、トリクロロエチレンを分解す
る。したがって、この微生物をトリクロロエチレンに汚
染された水又は土壌に接種・混合する際に、少なくとも
一種類以上の芳香族化合物を添加混合することによっ
て、安全かつ確実にトリクロロエチレンを分解除去する
ことができる。The microorganism according to the present invention decomposes trichlorethylene in water or soil in the presence of an aromatic compound. Therefore, when inoculating / mixing this microorganism into water or soil contaminated with trichlorethylene, by adding and mixing at least one kind of aromatic compound, trichlorethylene can be safely and reliably decomposed and removed.
【００１０】[0010]
【実施例】以下、本発明の実施例を添付図面を参照しな
がら詳細に説明する。本発明に係る微生物は、人工的に
調整したトリクロロエチレンを含有する汚染水に伊勢原
市産黒ボク土を長時間暴露させ、土壌の一部をバイアル
瓶に入れ、これに下記組成（培地１リットルあたり）の
無機培地、所定量のトリクロロエチレンおよびトルエン
を添加し、テフロンコートブチルゴムで栓をした後アル
ミキャップでシールし、３０℃で振蘯培養し、一定時間
経過後、倍ある瓶内の気相をガスクロマトグラフで分析
し、トリクロロエチレンの減衰が見られる試験系につい
て土壌量を希釈しながら集積を繰り返し、ＬＢ（Luria-
Bertani）ブロスに寒天を加えた平板培地に塗布し、出
現するコロニーの培養を繰り返して単離したものであ
る。なお、微生物の培養には、上記のＬＢブロスをはじ
め、トリプトソイブロス(Trypticase soy broth）等
任意に至適条件を選択してこれを行なうことが可能であ
る。Embodiments of the present invention will now be described in detail with reference to the accompanying drawings. Microorganisms according to the present invention, artificially adjusted contaminated water containing trichlorethylene exposed Isehara Kuroboku soil for a long time, put a portion of the soil in a vial, the following composition (per 1 liter of medium) ) Inorganic medium, a predetermined amount of trichlorethylene and toluene were added, and the container was sealed with Teflon-coated butyl rubber, sealed with an aluminum cap, shake-cultured at 30 ° C, and after a certain period of time, the gas phase in the double bottle was changed. Gas chromatograph analysis was performed, repeating accumulation while diluting the soil amount for the test system in which attenuation of trichlorethylene was observed, and LB (Luria-
Bertani) broth was applied on a plate medium containing agar, and colonies that appeared were repeatedly cultured and isolated. In addition, for culturing the microorganism, it is possible to arbitrarily select optimal conditions such as LB broth and trypticase soy broth.
【００１１】 Ｋ2ＨＰＯ4 １．０ｇ、ＫＨ2ＰＯ4 １．０ｇ ＮＨ4ＮＯ3 １．０ｇ、ＭｇＳＯ4・７Ｈ2Ｏ ０．２ｇ Ｆｅ2（ＳＯ4）3 ５ｍｇ、Ｎａ2ＭｏＯ4・Ｈ2Ｏ ５ｍｇ ＭｎＳＯ4・Ｈ2Ｏ ５ｍｇ、酵母エキス ５ｍｇK 2 HPO 4 1.0 g, KH 2 PO 4 1.0 g NH 4 NO 3 1.0 g, MgSO 4 .7H 2 O 0.2 g Fe 2 (SO 4 ) 3 5 mg, Na 2 MoO 4 .H 2 O 5 mg MnSO 4 · H 2 O 5 mg, yeast extract 5 mg
【００１２】そして、ガスクロマトグラフによる分析結
果によりトリクロロエチレン分解能の特に強い菌株を単
離し、その形態学的および生理学的性質を調べたところ
以下に示すような結果が得られた。Then, a strain having a particularly strong trichlorethylene decomposing ability was isolated from the results of analysis by gas chromatography, and its morphological and physiological properties were examined. The following results were obtained.
【００１３】 （ａ）形態 細胞の形状 短桿菌 大きさ １．１×１．６μｍ コロニーの性状 乳白色、発育旺盛（ＬＢ寒天培地にて） （ｂ）培養的性質 マッコンキー寒天培地上の育成 ＋ （ｃ）生理学的性質 グラム染色性 ＋ 硝酸還元能 ＋ インドールの生成 − オキシダーゼ試験 ＋ ウレアーゼ − クエン酸の利用 ＋ 炭素源資化性 デキストロース（好気条件下） −、デキストロース（嫌気条件下） − マルトース −、サッカロース − デンプン −、Ｄーキシロース − （ｄ）その他 フェニルアラニンの脱アミノ反応 − アルギニンの分解 − リジンの脱炭酸反応 − オルニチンの脱炭酸反応 − ＯＮＰＧ − 脂肪酸組成 １４：０ ２．４９％ １４：０ ２ＯＨ ３．８５％ １６：１ ３５．７０％ １６：０ ２０．７２％ １８：１ ２７．１１％(A) Morphology Cell shape Short bacillus Size 1.1 × 1.6 μm Colony properties Milky white, vigorous growth (on LB agar medium) (b) Culture properties Growth on MacConkey agar medium + (c ) Physiological properties Gram stainability + Nitrate reduction ability + Indole formation − Oxidase test + Urease − Utilization of citric acid + Carbon source-assimilating dextrose (aerobic condition) −, Dextrose (anaerobic condition) − Maltose −, Saccharose-starch-, D-xylose- (d) and others Deamination reaction of phenylalanine-Decomposition of arginine-Decarboxylation reaction of lysine-Decarboxylation reaction of ornithine-ONPG-Fatty acid composition 14: 0 2.49% 14: 0 2OH 3 .85% 16: 1 35.70% 16: 0 20.72% 18: 1 27.11%
【００１４】以上の結果から、単離されたトリクロロエ
チレン分解能の高い菌株は、アルカリジェネス エウロ
トロフス（Alcaligenes eurtrophus）KS01であることが
判明した。From the above results, it was found that the isolated strain with high degradability of trichlorethylene was Alcaligenes eurtrophus KS01.
【００１５】なお、この微生物は、寄託番号 ＦＥＲＭ
Ｐー１３７６１にて工業技術院微生物工業技術研究所
に寄託されている。The microorganism has a deposit number of FERM.
Deposited at Institute for Microbial Technology, Institute of Industrial Technology, P-13761.
【００１６】この微生物は、培地に混入した５０ppm程
度の比較的高濃度のトリクロロエチレンを完全に資化、
分解する。この微生物によりトリクロロエチレンを分解
させる際には、トリクロロエチレンを含む培地基（培
地、土壌、水等）中にトルエン等の芳香族化合物を少な
くとも一種類添加する必要がある。ここで用いられる芳
香族化合物としては、トルエン、フェノール、ｏ−クレ
ゾール、m-クレゾール等が用いられる。This microorganism completely assimilates a relatively high concentration of trichloroethylene of about 50 ppm mixed in the medium,
Disassemble. When degrading trichlorethylene with this microorganism, it is necessary to add at least one aromatic compound such as toluene to the medium substrate (medium, soil, water, etc.) containing trichlorethylene. As the aromatic compound used here, toluene, phenol, o-cresol, m-cresol, or the like is used.
【００１７】以下、本発明に係る微生物を、下記の実験
例によりさらに詳しく説明する。 実験例１ 容積６８mlのバイアル瓶に上記無機培地１５mlを入れ、
培養液中の濃度として、１００ppmのトルエンと、トリ
クロロエチレンとして、０．１〜５０ppmとなるように
調整する。そして、これにＬＢブロスで培養し遠沈洗浄
した本微生物を１０8個／ml（培地）接種した後、テフ
ロンコートブチルゴム栓をし、アルミキャップでシール
したものを３０℃で培養し、定期的に気相をＥＣＤ検出
器付きガスクロマトグラフで分析した。The microorganism according to the present invention will be described below in more detail with reference to the following experimental examples. Experimental Example 1 15 ml of the above inorganic medium was placed in a vial having a volume of 68 ml,
The concentration in the culture solution is adjusted to 100 ppm of toluene and 0.1 to 50 ppm of trichloroethylene. Then, after inoculating 10 8 cells / ml (medium) of this microorganism, which had been cultivated in LB broth and washed by centrifugation, a Teflon-coated butyl rubber stopper, which was sealed with an aluminum cap, was incubated at 30 ° C. The gas phase was analyzed by a gas chromatograph equipped with an ECD detector.
【００１８】この結果を図１に示す。同図に示したよう
に、培養液中の０．１〜５０ppm濃度のトリクロロエチ
レンは、５日以内に前記ガスクロマトグラフの検出限界
以下となり、本微生物が極めて高いトリクロロエチレン
分解能を有することが確認された。The results are shown in FIG. As shown in the figure, trichloroethylene at a concentration of 0.1 to 50 ppm in the culture solution was below the detection limit of the gas chromatograph within 5 days, and it was confirmed that the present microorganism has extremely high trichlorethylene decomposing ability.
【００１９】実験例２ 容積６８mlのバイアル瓶に上記無機培地１５mlを入れ、
培養液中の濃度として、１ppmとなるように、トリクロ
ロエチレンを添加した。これに、トルエン１〜１００pp
m、フェノール１ppmまたはフェノール１０ppmを滴下
し、これにＬＢブロスで培養し遠沈洗浄した本微生物を
１０8個／ml（培地）接種した後、テフロンコートブチ
ルゴム栓をし、アルミキャップでシールしたものを３０
℃で培養し、実験例１と同様に、定期的に気相をＥＣＤ
検出器ガスクロマトグラフで分析した。Experimental Example 2 15 ml of the above inorganic medium was placed in a vial having a volume of 68 ml,
Trichlorethylene was added so that the concentration in the culture solution would be 1 ppm. To this, toluene 1-100pp
m, Phenol 1ppm or Phenol 10ppm was added dropwise, and after inoculating 10 8 cells / ml (medium) of this microorganism that had been cultured in LB broth and spun down and washed, a Teflon-coated butyl rubber stopper was placed and sealed with an aluminum cap. 30
After culturing at 0 ° C., as in Experimental Example 1, the gas phase was ECD periodically.
It was analyzed by a detector gas chromatograph.
【００２０】図２は、この結果を示したものであり、同
図に示すように、全ての条件下で本微生物を接種後３日
程度で、トリクロロエチレンが前記ガスクロマトグラフ
の検出限界以下に分解されることが確認された。なお、
図には示していないが、トルエンに代えて、１若しくは
１０ppmのメタクレゾールまたはオルトクレゾールを混
合した場合にも、本微生物によってトリクロロエチレン
が確実に分解されることが確認された。FIG. 2 shows the results. As shown in FIG. 2, trichlorethylene was decomposed below the detection limit of the gas chromatograph within about 3 days after inoculation of the microorganism under all conditions. It was confirmed that In addition,
Although not shown in the figure, it was confirmed that trichloroethylene was surely decomposed by the present microorganism even when 1 or 10 ppm of metacresol or orthocresol was mixed in place of toluene.
【００２１】実験例３ 容積６８mlのバイアル瓶に前述の無機培地１５mlを入
れ、培養液中の濃度として、１ppmとなるようトリクロ
ロエチレンを添加し、これにトルエン１０ppmを滴下
し、これにＬＢブロスで培養し遠沈洗浄した本微生物を
１０9個／ml（培地）になるように接種した後、テフロ
ンコートブチルゴム栓をし、アルミキャップでシールし
たものを３０℃で培養し、１日後気相をトリクロロエチ
レンの場合にはＥＣＤ検出器付ガスクロマトグラフで、
トルエンの場合には、ＦＩＤ検出器付ガスクロマトグラ
フで分析した。Experimental Example 3 15 ml of the above-mentioned inorganic medium was placed in a vial having a volume of 68 ml, trichlorethylene was added so that the concentration in the culture solution would be 1 ppm, and 10 ppm of toluene was added dropwise to this, which was then cultured in LB broth. After inoculating the microorganism washed and spun down to 10 9 cells / ml (medium), a Teflon-coated butyl rubber stopper and an aluminum cap-sealed one were cultivated at 30 ° C., and after 1 day, the gas phase was trichlorethylene. In the case of, with a gas chromatograph with an ECD detector,
In the case of toluene, it was analyzed by a gas chromatograph with an FID detector.
【００２２】図３は、この結果を示したものであり、同
図に示すように、本微生物によって、トリクロロエチレ
ンが分解されるとともに、添加混合したトルエンも消失
していることが確認され、環境汚染物質であるトルエン
による環境汚染の心配がないことも確認された。FIG. 3 shows the results. As shown in FIG. 3, it was confirmed that trichloroethylene was decomposed by this microorganism and the added and mixed toluene was also disappeared. It was also confirmed that there is no concern about environmental pollution due to the substance toluene.
【００２３】次に、本発明に係る微生物を用いたトリク
ロロエチレンに汚染された土壌の浄化方法に関する実験
例ついて説明する。 実験例４ 容積１２４mlのバイアル瓶に伊勢原市黒ボク土（生土）
を１０ｇ（乾燥土）を入れるとともに、トリクロロエチ
レンを０．１ppmまたは１ppm含有する上記無機培地を２
０ml入れた後、これにさらにトルエンを１０ppm滴下
し、ＬＢブロスで培養し遠沈洗浄した本微生物を１０7
個／ml（培地＋生土）接種した後、テフロンコートブチ
ルゴム栓をして懸濁状態にし、さらにアルミキャップで
シールしたものを３０℃で培養し、定期的に気相をＥＣ
Ｄ検出器付ガスクロマトグラフで分析した。Next, an experimental example relating to a method for purifying soil contaminated with trichlorethylene using the microorganism of the present invention will be described. Experimental Example 4 In a vial with a volume of 124 ml, Isehara Kuroboku soil (raw soil)
10 g (dry soil) and 2 min of the above inorganic medium containing 0.1 ppm or 1 ppm of trichlorethylene.
After adding 0 ml, 10 ppm of toluene was further added dropwise to this, and the microorganism was cultured in LB broth and spun down for 10 7
After inoculating cells / ml (medium + raw soil), put a Teflon-coated butyl rubber stopper into a suspension state, and further seal with an aluminum cap, incubate at 30 ° C, and periodically subject the gas phase to EC.
It was analyzed by a gas chromatograph with a D detector.
【００２４】図４は、その結果を示すものであり、トリ
クロロエチレンエチレン濃度が０．１ppmの場合には３
日で、１ppmの場合にも１０日以内に前記ガスクロマト
グラフの検出限界以下に分解され、本微生物が自然環境
下でもトリクロロエチレン分解機能を発揮することが確
認された。これによって、滅菌状態の培養液中では効果
を示すが、自然界では土着の微生物に駆逐されてその分
解能を発揮できないことが多い既往の微生物にあって、
本微生物が、自然環境下でもその分解機能を十分に発揮
することから、汚染された水と接触させることにより、
また、汚染された土壌を本微生物を含む水と懸濁状態に
するなどしてこれらを浄化することが可能である。FIG. 4 shows the result, which is 3 when the concentration of trichlorethylene ethylene is 0.1 ppm.
Even in the case of 1 ppm per day, it was confirmed to be decomposed within the detection limit of the gas chromatograph within 10 days, and it was confirmed that the present microorganism exhibits the trichlorethylene decomposition function even in the natural environment. By this, although it shows an effect in a sterilized culture solution, in the existing microorganisms that are often unable to exert their decomposition ability by being exterminated by indigenous microorganisms,
Since this microorganism exerts its decomposition function sufficiently even in the natural environment, by contacting it with contaminated water,
Further, it is possible to purify the contaminated soil by suspending it in water containing the microorganism.
【００２５】[0025]
【発明の効果】本発明に係る微生物によれば、少なくと
も一種以上の芳香族化合物の存在下において、水または
土壌に含まれるトリクロロエチレンを数日以内に分解す
ることができる。According to the microorganism of the present invention, trichlorethylene contained in water or soil can be decomposed within a few days in the presence of at least one aromatic compound.
【００２６】本発明係る水または土壌の浄化方法によれ
ば、多大なエネルギーを必要とせず、また、二次汚染の
発生を抑さえることができる等のバイオ技術の利点が得
られるほか、自然環境下においてトリクロロエチレンに
汚染された水または土壌を工業的規模で効率良く浄化す
ることができる。The method for purifying water or soil according to the present invention does not require a large amount of energy, and has the advantages of biotechnology such as the ability to suppress the occurrence of secondary pollution, as well as the natural environment. Water or soil contaminated with trichlorethylene below can be efficiently purified on an industrial scale.
【図１】本発明に係る微生物の各種トリクロロエチレン
濃度における分解効果を示す実験結果である。FIG. 1 is an experimental result showing a decomposition effect of a microorganism according to the present invention at various concentrations of trichlorethylene.
【図２】本発明に係る微生物の分解効果を芳香化合物の
種類およびその濃度を変化させた場合について調べた実
験結果である。FIG. 2 is an experimental result of investigating the effect of degrading a microorganism according to the present invention when the type and concentration of an aromatic compound are changed.
【図３】本発明に係る微生物のトリクロロエチレンの分
解能およびトルエンの残存量をを示す実験結果であり、
（ａ）はトリクロロエチレンの初期ピーク、（ｂ）は一
日経過後のトリクロロエチレンのピーク、（ｃ）はトル
エンの初期ピーク、（ｄ）は一日経過後のトルエンのピ
ークである。FIG. 3 is an experimental result showing the ability of the microorganism of the present invention to decompose trichlorethylene and the residual amount of toluene,
(A) is the initial peak of trichlorethylene, (b) is the peak of trichlorethylene after one day, (c) is the initial peak of toluene, and (d) is the peak of toluene after one day.
【図４】本発明に係る微生物を汚染土壌に適用した場合
の分解効果を示す実験結果である。FIG. 4 is an experimental result showing a decomposition effect when the microorganism according to the present invention is applied to contaminated soil.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 //(Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:05） ─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. 6 Identification number Office reference number FI technical display area // (C12N 1/20 C12R 1:05)
Claims (3)
caligenes eurtrophus）に属し、トリクロロエチレン分
解能を有する微生物。1. Alkaline Genes Eurotrofus (Al
bacterium belonging to caligenes eurtrophus) and capable of degrading trichlorethylene.
ロトロフス（Alcaligenes eurtrophus）KS01（ＦＥＲＭ
Ｐー１３７６１）株である請求項１に記載の微生物。2. The microorganism is Alcaligenes eurtrophus KS01 (FERM).
The microorganism according to claim 1, which is a P-13761) strain.
物をトリクロロエチレンに汚染された水または土壌に接
種・混合して、これら水または土壌に含まれたトリクロ
ロエチレンを分解処理する水または土壌の浄化方法であ
って、前記水または土壌に前記微生物を接種・混合する
に際して、少なくとも一種以上の芳香族化合物を添加混
合することを特徴とする水または土壌の浄化方法。3. Purification of water or soil in which water or soil contaminated with trichlorethylene is inoculated and mixed with the microorganism according to claim 1 or 2 to decompose and treat trichlorethylene contained in the water or soil. A method for purifying water or soil, comprising adding and mixing at least one aromatic compound when inoculating and mixing the microorganisms into the water or soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27387793A JP3513715B2 (en) | 1993-11-01 | 1993-11-01 | Microorganisms and water or soil purification methods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27387793A JP3513715B2 (en) | 1993-11-01 | 1993-11-01 | Microorganisms and water or soil purification methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH07123976A true JPH07123976A (en) | 1995-05-16 |
| JP3513715B2 JP3513715B2 (en) | 2004-03-31 |
Family
ID=17533821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP27387793A Expired - Fee Related JP3513715B2 (en) | 1993-11-01 | 1993-11-01 | Microorganisms and water or soil purification methods |
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| Country | Link |
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| JP (1) | JP3513715B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5945331A (en) * | 1996-08-01 | 1999-08-31 | Canon Kabushiki Kaisha | Microorganisms, and method for biodegradation of organic compounds and method for environmental remediation |
| US5962305A (en) * | 1996-08-01 | 1999-10-05 | Canon Kabushiki Kaisha | Microbial strain, method for biodegrading organic compounds and method for environmental remediation |
| US6171844B1 (en) | 1996-08-19 | 2001-01-09 | Toyota Jidosha Kabushiki Kaisha | Microorganism and method for environmental purification using the same |
| US6472191B1 (en) | 1998-12-03 | 2002-10-29 | Canon Kabushiki Kaisha | Dna fragment carrying toluene monooxygenase gene, recombinant plasmid, transformed microorganism, method for degrading chlorinated aliphatic hydrocarbon compounds and aromatic compounds, and method for environmental remediation |
| US6660516B1 (en) | 1997-02-18 | 2003-12-09 | Canon Kabushiki Kaisha | Method for culturing a microorganism and promoting microbial growth and metabolism |
| US6864074B2 (en) | 1998-10-30 | 2005-03-08 | Canon Kabushiki Kaisha | Dna fragment carrying toluene monooxygenase gene, recombinant plasmid, transformed microorganism, method for degrading chlorinated aliphatic hydrocarbon compounds and aromatic compounds, and method for environmental remediation |
-
1993
- 1993-11-01 JP JP27387793A patent/JP3513715B2/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5945331A (en) * | 1996-08-01 | 1999-08-31 | Canon Kabushiki Kaisha | Microorganisms, and method for biodegradation of organic compounds and method for environmental remediation |
| US5962305A (en) * | 1996-08-01 | 1999-10-05 | Canon Kabushiki Kaisha | Microbial strain, method for biodegrading organic compounds and method for environmental remediation |
| US6171844B1 (en) | 1996-08-19 | 2001-01-09 | Toyota Jidosha Kabushiki Kaisha | Microorganism and method for environmental purification using the same |
| US6521444B1 (en) | 1996-08-19 | 2003-02-18 | Toyota Jidosha Kabushiki Kaisha | Microorganism and method for environmental purification using the same |
| US6660516B1 (en) | 1997-02-18 | 2003-12-09 | Canon Kabushiki Kaisha | Method for culturing a microorganism and promoting microbial growth and metabolism |
| US6864074B2 (en) | 1998-10-30 | 2005-03-08 | Canon Kabushiki Kaisha | Dna fragment carrying toluene monooxygenase gene, recombinant plasmid, transformed microorganism, method for degrading chlorinated aliphatic hydrocarbon compounds and aromatic compounds, and method for environmental remediation |
| US6472191B1 (en) | 1998-12-03 | 2002-10-29 | Canon Kabushiki Kaisha | Dna fragment carrying toluene monooxygenase gene, recombinant plasmid, transformed microorganism, method for degrading chlorinated aliphatic hydrocarbon compounds and aromatic compounds, and method for environmental remediation |
| US6858417B2 (en) | 1998-12-03 | 2005-02-22 | Canon Kabushiki Kaisha | Dna fragment carrying toluene monooxygenase, gene, recombinant plasmid, transformed microorganism, method for degrading chlorinated aliphatic hydrocarbon compounds and aromatic compounds, and method for environmental remediation |
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|---|---|
| JP3513715B2 (en) | 2004-03-31 |
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