JPH07109281A - Optically active (r)-n-piperonyl-1,2,3,4-tetrahydrobenzo(b)thieno(2,3-c)pyridine-3-carboxamide, production and use thereof - Google Patents

Abstract

PURPOSE:To provide the compound useful as a psychopharmaceutical having an antianxiety effect and a learning-improving effect. CONSTITUTION:A compound of formula I (* is asymmetric carbon) or its acid adduct. The compound is obtained by subjecting a compound of formula II and formalin to a cyclization reaction in the presence of a catalyst (e.g. hydrochloric acid) in a solvent (e.g. water-methanol) at 10-IOOC 1-30 hr. The (R)-N-piperonyl-1,2,3,4-tetrahydrobenzo[b]thieno[2,3-c]pyridin-3-carbo xamide hydrochloride has a stronger antianxiety action than that of the (S)-N- piperonyl-1,2,3,4-tetrahydrobenzo[b]thieno[2,3-c]pyridine-3-carboxamide hydrochloride, and is low in hepatic toxicity, suitable as a psychopharmaceutical, and strong in a serotonin 1A-binding ability which is considered to be the main action mechanism of the medicinal effect.

Description

Detailed Description of the Invention

[0001]

The present invention relates to the following compounds as a psychotropic drug which affects central nervous system of mammals and has anxiolytic effect and learning improving effect.

[0002]

[Chemical 6]

An optically active DN-piperonyl-1,2,3,4-tetrahydro-benzo [b] thieno [2,3-c] pyridine-3-carboxamide represented by ## STR2 ## And a method for producing the same, and a therapeutic agent containing the same.

[0004]

The following compounds

[0005]

[Chemical 7]

N-piperonyl-1,2, represented by
3,4-Tetrahydro-benzo [b] thieno [2,3-
c] Pyridine-3-carbamide (racemic compound), a process for producing the same, and usefulness of the drug containing the same as a psychotropic drug having an anxiolytic effect and a learning improving effect have been filed by the present applicant. (Japanese Patent Application No. 4-126
706).

[0007]

However, most of compounds having an asymmetric carbon in the molecule have optical isomers having almost the same physicochemical properties. It has been clarified that the separation and quantification of optical isomers have been facilitated by recent advances in analytical chemistry and research has progressed, and in many cases, their behaviors in vivo are different. That is, the two optical isomers have different drug effects and toxicity, and there is a problem that the less useful isomer is an impurity.

DN-piperonyl-1, which relates to the present invention,
2,3,4-tetrahydro-benzo [b] thieno [2,2
3-c] Pyridin-3-carboxamide, a method for producing the same, and a drug containing the same are compounds having an asymmetric carbon, and have two optical isomers. When this compound is used as a medicine, it is considered preferable to use either one of the optically active substances.

[0009]

In order to solve the above-mentioned problems, the inventor of the present invention has made earnest studies and completed the present invention. That is, the present invention provides an optically active DN-piperonyl-
The present invention relates to 1,2,3,4-tetrahydro-benzo [b] thieno [2,3-c] pyridine-3-carboxamide, a method for producing the same, a drug containing the same, and an acid addition salt thereof.

The compound of the present invention is prepared by the following production method 1.

[0011]

[Chemical 8]

According to α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl)-
The L-form is deacylated by hydrolysis of propionic acid (2) with an enzyme that hydrolyzes α-acyl amino acid such as acylase to obtain L-2-amino- (benofen-3-yl).
-Propionic acid (3) was used, and the D-form was left undegraded to form D-
Leaving as α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid (4) gives two optically active intermediates. Furthermore, when describing this production method in detail, 1 .. of racemic form of α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid (2).
0% to 70% (W / V) aqueous solution, preferably 5.0-
40% (W / V) with sodium hydroxide to pH 3.0-1
The reaction temperature is 5.0 to 60 after the addition of acylase to an aqueous solvent adjusted to about pH 1, preferably pH 5.5 to 7.0.
° C, preferably 30 to 45 ° C, reaction time 0.5 to 30
By stirring for 0 hour, preferably 5.0 to 24 hours, only the L-form of α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid (2) was obtained. Convert to L-2-amino- (benzo [b] thiophen-3-yl) -propionic acid (3). The acylase used in the present invention is one that hydrolyzes only the L-form of α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid (2). , Those obtained from any kind of bacterial cells may be used. For example, the filamentous fungi Aspergillus, Penicillium, bacteria Pseudomonas, Achromobacter, Micrococcus, and actinomycete Streptomyces are used. The amount of acylase used varies depending on the concentration of α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid (2), but α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl)-
The amount is preferably 0.1 to 100% by weight, particularly preferably 0.5 to 2.0% by weight, based on propionic acid (2). Further, in the present invention, cobalt may coexist in order to accelerate the reaction and further stabilize it. Any cobalt salt can be used as the cobalt to be used as long as it is soluble in water, such as cobalt chloride, cobalt sulfate and cobalt acetate. And the concentration is in the range of 0.001 to 100 mM.
As the aqueous solvent used in the present invention, various buffer solutions of water, and an organic solvent mixed with water, for example, methanol may be appropriately added. The reaction-terminated liquid obtained by the above method contained L-2-amino- (benzo [b]
Unreacted D-α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl)-with high solubility of thiophen-3-yl) -propionic acid (3) precipitated as crystals. Propionic acid (4) remains as a solution. By solid-liquid separation of this reaction solution, for example, filtration, L-2-amino- (benzo [b] thiophen-3-yl) -propionic acid (3) having a high yield and a high optical purity can be obtained. On the other hand, the pH of the solid-liquid separated solution
Of unreacted D-α
Crystals of -acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid (4) can be obtained with high purity. This intermediate is further derivatized to give D-N-piperonyl-1,2,3,3.
4-Tetrahydro-benzo [b] thieno [2,3-c]
Pyridine-3-carboxamide (1) and L-N-piperonyl-1,2,3,4-tetrahydro-benzo [b] thieno [2,3-c] pyridine-3-carboxamide (5)
Lead to. Here, the derivatization specifically means that a process such as a manufacturing method 2 described below is performed. The drug efficacy and toxicity were investigated using these two optical isomers. D
The -body has a stronger anxiolytic effect, which is a medicinal effect, the serotonin _1A binding ability, which is considered to be the main mechanism of its medicinal effect, is significantly stronger, and the liver toxicity is lower and excellent than the L-body. Examples of the pharmaceutically acceptable acid addition salts include inorganic acid such as hydrochloric acid, sulfuric acid and phosphoric acid, and acid addition salt of organic acid such as mandelic acid, p-toluenesulfonic acid and maleic acid. .

In the above production method 1, the compound (4)
The process for producing the compound (1) from is further detailed as the following production method 2.

[0014]

[Chemical 9]

In the production method 2, D-2-amino-
(Benzo [b] thiophen-3-yl) -propionic acid (6) is D-α-acetamino-α-carboxy-β-.
(3-Benz [b] thiophen-3-yl) -propionic acid (4) can be produced by hydrolyzing an acyl group with an acid without racemization. As the acid to be used, inorganic acids such as hydrochloric acid and sulfuric acid, organic acids such as methanesulfonic acid and trifluoroacetic acid, and the like can be used. The concentration is 0.1 to 18 N, preferably 1 to 12 N.

To protect the amino group used in the reaction of compound (6) to compound (7), a urethane-type protecting group such as t-butoxycarbonyl group, benzyloxycarbonyl group, acetyl group, formyl group, trifluoroacetyl, etc. An acyl type protecting group such as a group is used. For example, when protected with a t-butoxycarbonyl group, it can be obtained by the following synthetic method.

The method for synthesizing the compound (6) to the compound (7) is carried out by converting the optically active (6) into an ether such as tetrahydrofuran.
Dissolved in a mixed solvent of tellurium-based solvent and water, di-t-butyl-
After adding 1 to 12 equivalents (preferably 1 to 2 equivalents) of di-carbonate and triethylamine, the mixture is reacted at room temperature. As a method for synthesizing the compound (8) from the compound (7), a general method for synthesizing an amide from a carboxylic acid and an amine can be applied. For example, a method of using a condensing agent such as DCC (dicyclohexylcarbodiimide), a method of condensing a carboxylic acid into an acid chloride, and the like can be mentioned.

The method using DCC can be performed by the following method, for example. Compound (7) is dissolved in a halogenated solvent such as chloroform and 1-hydroxybenzotriazole is added. At this time, 1-hydroxybenzotriazole is 1 to 10 equivalents, preferably 1.1 to 2 equivalents. In addition, piperonylamine and 1,3-dicyclohexylcarbodiimide are added. At this time, piperonylamine and 1,3-dicyclohexylcarbodiimide are 1 to 10 equivalents, preferably 1.1 to 2 equivalents. The final dicyclohexylurea formed is removed by filtration.

As a method for synthesizing the compound (9) from the compound (8), a method using an acid or a method using catalytic reduction can be applied. In this case, an acid method is preferable because it has a sulfur atom in the molecule. Commonly used acids such as methanesulfonic acid, hydrochloric acid and sulfuric acid can be used. For example, 4N hydrochloric acid in 1,4-dioxane solution is added to the compound (8) and reacted. At this time, the amount of the acid is 1 to 100 equivalents, preferably 2 to 5 equivalents. The reaction temperature is 0 to 155 °
C, preferably 60 to 90 ° C.

The method for synthesizing the compound (1) from the compound (9) is described in Dee. Zoelens et al., Journal of
Organic Chemistry (D. Soerens e
t. al. J. Org. Chem. , 44 (4), 53
5-545 (1979)) was referred to. Compound (9)
On the other hand, formalin is usually used in an amount of 1 to 10 equivalents, preferably 1.5 to 2 equivalents. The solvent used in this method is preferably a mixed solvent of water and a lower alcohol solvent such as methanol, ethanol, propyl alcohol and hexyl alcohol. The reaction temperature is 10 to 100 ° C.
At 50 to 70 ° C. The reaction time is generally 1 to 30 hours, preferably 20 to 2
5 hours. Further, as a reaction catalyst, 1 to 10 equivalents of an acid such as hydrochloric acid, sulfuric acid, p-toluenesulfonic acid or the like is used.

[0021]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto.

[0022]

Example 1 500 ml of tap water and α-acetamino-
α-carboxy-β- (3-benzo [b] thiophene-
To a slurry consisting of 150 g of 3-yl) -propionic acid, 4N-sodium hydroxide solution was added and dissolved to give a final p
It was adjusted to H6.5. To this solution, 1.0 g of acylase (30000 units of Amano Pharmaceutical Co., Ltd.) and 0.0 of cobalt chloride hexahydrate
72 g was added, and the mixture was stirred at 38 ° C. and pH 6.5 for 24 hours. Crystals of L-2-amino- (benzophen-3-yl) -propionic acid precipitated in the reaction solution were filtered and washed with water, and then the crystals were dried to give white L-2-amino- (benzophen- 39.7 g of 3-yl) -propionic acid crystals
Got This crystal was used as a gas chromatography industry HPLC column Unisil Pack F3-50A, eluent 35% methanol /
As a result of analysis with 50 mM KH ₂ PO ₄ , the purity was 99.9.
It was 5%. [Α] ^D ₂₅ = 9.17 ° (C0.6,
0.1 N-HCl), optical isomer separation column manufactured by Daicel Chemical Co., Ltd., Crown pack CR (+), pH of eluent
As a result of examining the optical purity with 4.0% methanol water of 4.0,
It was confirmed that the L-form was 100% pure. Analysis result of L-2-amino- (benzofen-3-yl) -propionic acid IR (ν _max , cm ⁻¹ ): 3030, 1665, 159
On the other hand, concentrated hydrochloric acid was added to about 500 ml of the separated mother liquor to adjust the pH to 3.
It was reduced to 0 and crystallized. After obtaining crystals by filtration, 0.01
The crystals washed with N-HCl are dried to give white, D-α.
-Acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid crystal 7
3.5 g was obtained.

The crystals were analyzed with Unisil Pack F3-50A, a gas chromatography industry HPLC column, and 35% methanol / 50 mM-KH ₂ PO _{4 as} an eluent, and as a result, the purity was 99.91%. Next, Daicel Chemical Industries, Ltd. optical isomer separation column Chiralcel OJ, eluent hexane: 2-
As a result of examining the optical purity with propanol: trifluoroacetic acid 90: 10: 0.1, the D-form was 99.7%. D
-Α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid 0.5
5 mL of 5N-methanesulfonic acid was added to g, and the mixture was stirred at 100 ° C. for 6 hours and 30 minutes. After returning to room temperature and adding 20 mL of water, the pH was adjusted to 6.5 with 6N-sodium hydroxide. The precipitated crystals were collected by filtration and dried, and D-2-amino-
(Benzofen-3-yl) -propionic acid 0.41 g
Got (Yield 93.0%) Analysis result of D-2-amino- (benzophen-3-yl) -propionic acid IR (ν _max , cm ⁻¹ ): 3050, 1670, 158
5

[0024]

Example 2 53.5 g of D-2-amino- (benzophen-3-yl) -propionic acid was added to tetrahydrofuran 3
It was dissolved in 50 ml of water and 350 ml of water, cooled to 0 ° C., and 67.4 g of di-t-butyl-di-carbonate was added.
Further, at room temperature, 41 ml of triethylamine was added, and 4
After stirring for an hour, the solvent was concentrated under reduced pressure and then ethyl acetate 500 was added.
The organic layer was washed with 100 ml of water and 500 ml of saturated saline and then dried over sodium sulfate. The organic layer is dried over magnesium sulfate,
After concentration under reduced pressure to dryness, D-2-butoxycarbonylamino- (benzophen-3-yl) -propionic acid 77.
4 g was obtained. (Yield 100%) NMR (δ, DMSO-d6): 1.00 to 1.57
(M, 9H), 3.08 to 3.40 (m, 2H), 4.
21 to 4.52 (m, 1H), 7.12 to 8.09
(M, 5H) IR (ν _max , cm ^-1 ): 3440, 2940, 170
0, 1635, 1560

[0025]

Example 3 D-2- (t-Butoxycarbonylamino)-(benzophen-3-yl) -propionic acid 5
8.7 g, 1-hydroxybenzotriazole 24.7
g, piperonylamine 19.4 ml with chloroform 82
It was dissolved in 0 ml, 41.4 g of dicyclohexylcarbodiimide was added at 0 ° C, and the mixture was stirred at room temperature for 1 hour. The precipitated dicyclohexylurea was removed by filtration. It was washed with 300 ml each of a saturated sodium hydrogen carbonate solution, a 10% citric acid solution and a saturated saline solution, and dried with sodium sulfate. The solvent is distilled off so that the amount of the solvent becomes about 300 ml,
500 ml of ether was added and the mixture was left standing for 16 hours. The precipitated crystals were collected by filtration, and DN-piperonyl-2- (t-butoxycarbonylamino)-(benzofen-3-yl) was used.
55.4 g (yield 67%) of propionylamide was obtained.

NMR (δ, CDCl ₃ ): 1.36
(S, 9H), 3.29 (d, 2H, J = 7.5H
z), 4.13 (d, 2H, J = 7.5 Hz), 4.4
3 (q, 1H, J = 7.5 Hz), 5.97 (s, 2
H), 6.00-6.30 (m, 1H), 6.30-
6.70 (m, 3H), 7.10 to 7.90 (m, 5
H) IR (ν _max , cm ^-1 ): 3300, 1680, 164
5, 1565, 1520

[0027]

Example 4 63.7 g of DN-Piperonyl-2- (t-butoxycarbonylamino)-(benzophen-3-yl) -propionylamide was added to 500 ml of dioxane. Furthermore, at room temperature, 4m dioxane hydrochloride 142m
1 was added, and the mixture was stirred at 80 ° C for 1 hour. 15 at 60 ° C
When the organic solvent is concentrated under reduced pressure to 0 ml, crystals start to precipitate. The crystals were collected by filtration to obtain 52.3 g of DN-piperonyl-2-amino- (benzophen-3-yl) -propionylamide hydrochloride (yield 95%).

NMR (δ, DMSO-d6): 3.20
Up to 3.40 (m, 2H), 4.00 to 4.20 (m, 3
H), 5.90 (s, 2H), 6.61 (dd, 1H,
J = 7.9 Hz, J = 1.3 Hz), 6.72 (d, 1
H, J = 1.3 Hz), 6.78 (d, 1H, J = 7.
9 Hz), 7.30 to 8.10 (m, 5H), 8.51
(Bs, 3H), 9.04 (m, 1H) IR (ν _max , cm ⁻¹ ): 3250, 2970, 166
0,1550

[0029]

Example 5 DN-Piperonyl-2-amino- (benzophen-3-yl) -propionylamide hydrochloride 1
Dissolve 0.0 g in 50 ml of methanol and 50 ml of water,
32 ml of 5N hydrochloric acid was added. 35% formalin 6.55
ml was added, and the mixture was stirred at 70 to 90 ° C for 7 hours. 0-1
Upon cooling to 0 ° C., crystals were precipitated and collected by filtration. Recrystallized from methanol-ether to give DN-piperonyl-1,
2,3,4-Tetrahydrobenzo [b] thieno [2,3
-C] Pyridine-3-carboxamide hydrochloride 9.67 g
Was obtained (yield 94%).

NMR (δ, CD ₃ OD): 2.33 ~
4.12 (m, 5H), 5.93 (s, 2H), 6.7
7 to 8.07 (m, 9H) IR (ν _max , cm ⁻¹ ): 3250, 2900, 168
5, 1435, 760

[0031]

Example 6 L-N-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride is the D-2-amino-form of Example 2. (Benzofen-3-yl) -propionic acid 53.
5.11 g was obtained in exactly the same manner except that 53.5 g of L-2-amino- (benzophen-3-yl) -propionic acid was used instead of 5 g (however, the final yield was 89%.
Only listed). Analysis result of L-N-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride NMR (δ, CD ₃ OD): 2.33- 4.12 (m,
5H), 5.93 (s, 2H), 6.77 to 8.07.
(M, 9H) IR (ν _max , cm ⁻¹ ): 3250, 2900, 168
5, 1435, 760 and then DN-piperonyl-1,
2,3,4-Tetrahydrobenzo [b] thieno [2,3
-C] pyridine-3-carboxamide hydrochloride is L-N-
The following tests show that it is superior to piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride. (2 Week Oral Repeated Dose Toxicity Test) Crj: Wistar rat ♂, N-piperonyl-
1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride in D- and L-forms of 100, 200 and 300 m, respectively.
Five g / kg each were continuously administered for 2 weeks, and the effect on the liver was observed. The test results are shown in Table 1. From the results in Table 1,
Abnormalities such as swelling of the liver were observed in the L-body at 100 mg / kg, while there was no abnormality in the liver in the D-body at 100 mg / kg. The D-form is less toxic to the liver than the L-form and is considered to be a safer compound. (Anxiolytic effect) Using Wistar male rats (6 weeks old), Vogel. J. Earl and Bear. Bee, Clodie. Dee. E, Psycho Pharmacologia (Vo
gelJ. R. Beer B .; , And Clody
D. E. , Psychopharmacologi
a) Water Conflict Test (Water lic) based on 1-7, 21 (1971)
k conflict test) to confirm the anxiolytic effect of D- and L-forms of N-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride. Examined.

In this test, dehydrated rats are allowed to drink water.
The drug was administered 4 to 5 hours later, and the test was started after the treatment time. The number of shocks is the number of electric shocks that a rat receives only water for 5 minutes, and indicates whether or not drinking water suppresses the conflict (anxiety) of receiving an electric shock. That is, an increase in the number of shocks means that the anxiolytic effect is enhanced. Table 2 shows the values when the drug-non-administered rats were set to 100. From the results in Table 2, it is clear that the D-form has a significantly stronger anxiolytic effect than the L-form, and the D-form is L-form in terms of the pharmacological effect.
-Indicates that it is significantly superior to the body. (Serotonin _1A binding ability test) (1) Method for adjusting serotonin 1A receptor membrane Sprague-Dawley male rat (7 weeks old,
After decapitating (Charles River), the cerebrum was immediately removed and the hippocampus was extracted under ice cooling. Tissue is 50 mM Tris-
The mixture was homogenized with HCl buffer (pH 7.4) and then centrifuged (48,000 xg, 15 min). The precipitate was resuspended in the above buffer and incubated at 30 ° C. for 20 minutes (decomposition of endogenous 5-HT), 4
It was centrifuged at 8,000 xg for 15 minutes. The obtained sediment is b
indicing buffer (50 mM Tris-H
Cl, 2.5 mM MgCl ₂ pH 7.4) was suspended to obtain a membrane preparation. (2) Experiments and Results 0.5 nM ³ H-8-OH- with a total volume of 1 ml of the membrane preparation.
Incubated with DPAT and 10 nM pargyline (MAO inhibitor) at 30 ° C. for 30 minutes. The measurement of non-specific binding while changing the drug concentration was performed in the presence of 10 μM serotonin, and the value obtained by subtracting this value from the measurement value in the absence of serotonin was used for specific 3H-8-OH-D.
PAT binding was used. After the completion of the incubation, the reaction solution was suction filtered with a glass fiber filter paper (Whatman GF / C), and the filter paper was mixed with 4 ml of binding buffer to 3 times.
After washing twice, it was transferred to a vial bottle and a scintillator was added to measure radioactivity. The results are shown in Table 3. From the results of Table 3, the D-form was more selectively bound to serotonin _1A than the L-form. As shown in Test Example 2, the anxiolytic effect was D-.
It is suggested from the mechanism of action that the body is stronger than the L-body because it has a strong binding ability to serotonin _1A . From the results of the above-mentioned usefulness test examples 1, 2, and 3, it is clear that DN-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide. It is clear that the hydrochloride salt is superior to LN-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride salt.

[0033]

[Table 1]

[0034]

[Table 2]

[0035]

[Table 3]

[0036]

ADVANTAGES OF THE INVENTION DN-Piperonyl-1,2,3,4-
Tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride is L-N-piperonyl-
1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride is superior in toxicity and drug efficacy to the DN-piperonyl-
1,2,3,4-Tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide has a strong anxiolytic effect when administered to humans or animals in the free form or in the form of its salt. It is suitable as a psychotropic drug because it exhibits low toxicity.

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[Procedure amendment]

[Submission date] May 19, 1994

[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] Full text

[Correction method] Change

[Correction content]

[Document name] Statement

Title: Optically active (R) -N-piperonyl-
1,2,3,4-Tetrahydro-benzo [b] thieno [2,3-c] pyridine-3-carboxamide , its production method and its use

[Claims]

[Chemical 1] (In the formula, * represents an asymmetric carbon atom) , and the optically active (R) -N-piperonyl-1,2,3,3
4-Tetrahydro-benzo [b] thieno [2,3-c]
Pyridine-3-carbamide or an acid addition salt thereof.

[Chemical 2] (In the formula, * represents an asymmetric carbon atom)
Optically active compound or its acid addition salt and formalin
Formula (1) characterized by reacting

[Chemical 3] (In the formula, * indicates an asymmetric carbon atom)
Optically active (R) -N-piperonyl-1,2,3,3
4-Tetrahydro-benzo [b] thieno [2,3-c]
Production of pyridine-3-carboxamide or acid addition salt thereof
Law.

[Chemical 4] The piperonylamine represented by the general formula (5)

[Chemical 5] (In the formula, B represents an amino-protecting group, and * represents an asymmetric carbon atom.
An optically active carboxylic acid represented by
Alternatively, the compound of general formula (3) can be obtained by acylating with a reactive derivative thereof.

[Chemical 6] (In the formula, B represents an amino-protecting group, and * represents an asymmetric carbon atom.
An optically active compound represented by
Then, the protecting group of the amino group of the compound is eliminated to give a compound of formula (2)

[Chemical 7] (In the formula, * indicates an asymmetric carbon atom)
To obtain an optically active compound or acid addition salt thereof,
Formula (1) characterized by reacting formalin

[Chemical 8] (In the formula, * represents an asymmetric carbon atom) , and the optically active (R) -N-piperonyl-1,2,3,3
4-Tetrahydro-benzo [b] thieno [2,3-c]
Process for producing pyridine-3-carboxamide or acid addition salt thereof .

[Chemical 9] (In the formula, * indicates an asymmetric carbon atom)
Optically active (R) -N-piperonyl-1,2,3,3
4-Tetrahydro-benzo [b] thieno [2,3-c]
Effective with pyridine-3-carbamide or its acid addition salts
An anxiolytic agent characterized by being contained as an ingredient.

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an optically active (R) -N-piperonyl-1, which is useful as a psychotropic drug, which has an anxiolytic effect and a learning improving effect on the central nervous system of mammals.
2,3,4-tetrahydro-benzo [b] thieno [2,2
3-c] pyridine-3-carboxamide , its production method and
And its use .

[0002]

2. Description of the Related Art Conventionally, general formula

[0003]

[Chemical 10]

(In the formula, A is a sulfur atom or an oxygen atom, R is
₁ is a hydrogen atom, R ₂ is a hydrogen atom, methyl, ethyl, 2-
Aminoethyl, 3-aminopropyl, 2-dimethylamido
Noethyl, N, N-dicyclohexylaminocarbonyl
Or represents a cyclohexyl group, or R ₁ and R
₂ is at least one nitrogen atom along with adjacent nitrogen atoms
A 6 to 7 membered heterocyclic group having R ₃ and R ₄
Halogen atom, unsubstituted or substituted alkyl group,
Reel group, alkenyl group, acyl group or arylcarl
Represents a bonyl group, m and n are integers from 0 to 4, and m
And n is 2 or more, R ₃ and R ₄ are the same.
Which may be one or different)
Derivatives affect the central nervous system of mammals and have anxiolytic effects.
As a result, it is useful as a psychotropic drug with learning improvement effect.
Is known (Japanese Patent Laid-Open No. 2-149583).

[0005]

However, most of compounds having an asymmetric carbon in the molecule have optical isomers having almost the same physicochemical properties. It has been clarified that the separation and quantification of optical isomers have been facilitated by recent advances in analytical chemistry and research has progressed, and in many cases, their behaviors in vivo are different. That is, the two optical isomers have different drug effects and toxicity, and there is a problem that the less useful isomer is an impurity.

[0006] that involved in the present invention N - piperonyl-1,2,
3,4-Tetrahydro-benzo [b] thieno [2,3-
c] Pyridine-3-carboxamide or an acid addition salt thereof is a compound having an asymmetric carbon atom, and has two optical isomers. When this compound is used as a medicine, it is considered preferable to use either one of the optically active substances.

[0007]

Means for Solving the Problems The inventors of the present invention have conducted extensive studies in order to solve the above problems, and as a result, the following formula (1) is obtained.
An optically active (R) -N-piperonyl-1,
2,3,4-tetrahydro-benzo [b] thieno [2,2
3-c] Pyridin-3-carboxamide or acid addition thereof
Salt has a stronger anxiolytic effect than the other optically active substance, and
It was found that the toxicity is low and the present invention has been completed .

That is, the present invention uses the formula (1)

[Chemical 11]

(In the formula, * represents an asymmetric carbon atom.
Represented by you) optically active (R)-N-piperonyl -
1,2,3,4-tetrahydro-benzo [b] thieno [2,3-c] pyridine-3-carboxamide (hereinafter
“Compound (1)” or an acid addition salt thereof is provided.

Further , the present invention provides the formula (2)

[Chemical 12] (In the formula, * represents an asymmetric carbon atom)
Optically active compound (hereinafter referred to as "compound (2)"
) Or its acid addition salt with formalin
Of the compound (1) or an acid addition salt thereof
Is provided.

Furthermore, the present invention provides the formula (4)

[Chemical 13]

The piperonylamine represented by the general formula
(5)

[Chemical 14]

(In the formula, B represents an amino-protecting group,
Is an asymmetric carbon atom)
Carboxylic acid (hereinafter referred to as "carboxylic acid (5)")
Alternatively, the compound of general formula (3) can be obtained by acylating with a reactive derivative thereof.

[Chemical 15]

(In the formula, B represents an amino-protecting group,
Is an asymmetric carbon atom)
Compound (hereinafter referred to as “compound (3)”)
The protecting group for the amino group of the compound is eliminated to give the compound (2).
Or its acid addition salt is obtained and then reacted with formalin
A compound (1) or an acid addition salt thereof
It provides a construction method.

Further, the present invention provides compound (1) or a compound thereof.
Antioxidant characterized by containing an acid addition salt as an active ingredient
It provides anxiety agents.

Starting materials for the production of the compound (1) of the present invention
The optically active calcium represented by the general formula (5)
Boronic acid (hereinafter referred to as “carboxylic acid (5)”) is, for example,
For example, it is manufactured by the following method.

Formula (9)

[Chemical 16] The racemic α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl)-represented by
And the α- acylamino acid acylase such as propionic acid (9) was deacylated hydrolyze L- body by the action of an enzyme hydrolyzing the formula (8)

[0018]

[Chemical 17] (In the formula, * represents an asymmetric carbon atom)
That L-2-amino - (benzo [b] thiophen-3-yl) - and propionic acid (8), Mom formula undecomposed the D- body (7)

[0019]

[Chemical 18] (In the formula, * represents an asymmetric carbon atom)
D-alpha-acetamino -α- carboxy -β- that (3-
Ben zo [b] thiophen-3-yl) - left as propionic acid (7), to obtain two kinds of optically active intermediates.

The enzymatic reaction described above, the racemic α- acetamino -α- carboxy-.beta.-(3- benzo [b] thiophen-3-yl) - 1 propionic acid (9). 0% to 70% (W / V) aqueous solution, preferably 5.0-40%
After the acylase was added to an aqueous solvent whose (W / V) was adjusted to about pH 3.0 to 11 with sodium hydroxide, preferably pH 5.5 to 7.0, the reaction temperature was 5.0 to 60 ° C.
By stirring preferably at 30 to 45 ° C. for a reaction time of 0.5 to 300 hours, preferably 5.0 to 24 hours,
α- acetamino -α- carboxy-.beta.-(3- benzo [b] thiophen-3-yl) - only the L-form of the propionic acid (9) is L-2-amino - (Ben zo [b] thiophen-3 Yl) -propionic acid ( 8 ).

[0021] acylase to be used in the enzyme reaction, La
Semi-form α-acetamino-α-carboxy-β- (3
As long as it hydrolyzes only the L-form of -benzo [b] thiophen-3-yl) -propionic acid ( 9 ), it may be obtained from any kind of bacterial cell. For example,
Filamentous fungi such as Aspergillus, Penicillium, Pseudomonas, Achromobacter, Micrococcus, and Streptomyces of actinomycetes are used. The amount of acylase used is racemic α-acetamino-α-
Carboxy-β- (3-benzo [b] thiophene-3-
Α) -propionic acid ( 9 )
-Acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid ( 9 ) 0.1 to 100% by weight, particularly 0.5 to 2.0% by weight
A degree is preferable.

Cobalt may be coexistent in order to promote and further stabilize the above reaction. The cobalt used is
Any cobalt salt can be used as long as it is soluble in water, such as cobalt chloride, cobalt sulfate, and cobalt acetate. And the concentration is in the range of 0.001 to 100 mM. As the aqueous solvent used in the present invention, various buffer solutions of water, and an organic solvent mixed with water, for example, methanol may be appropriately added. The reaction-terminated liquid obtained by the above method contains L-2-amino-
(Ben zo [b] thiophen-3-yl) - propionic acid (8) is precipitated as crystals, the solubility of the large unreacted D
-Α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid ( 7 )
Remains as a solution. This reaction solution to solid-liquid separation, for example, high optical purity in high yield by filtration L-2-amino - (Ben zo [b] thiophen-3-yl) - can obtain the propionic acid (7) .

On the other hand, by adjusting the pH of the solid-liquid separated solution to 3 or less with hydrochloric acid or the like, unreacted D-α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl is obtained. ) -Propionic acid ( 7 ) (hereinafter referred to as "compound
The crystal of the product (7) ” can be obtained with high purity.

By hydrolyzing the acyl group of the above compound (7) with an acid without causing racemization,
Formula (6)

[Chemical 19] (In the formula, * represents an asymmetric carbon atom)
Optically active D-2-amino- (benzo [b] thiophene
N-3-yl) -propionic acid (hereinafter, “D-amino acid
(6) ") can be manufactured . As the acid to be used, inorganic acids such as hydrochloric acid and sulfuric acid, organic acids such as methanesulfonic acid and trifluoroacetic acid, and the like can be used. The concentration is 0.1 to 18 N, preferably 1 to 12 N.

D-amino acid thus obtained
In (6), the amino group is protected with an amino protecting group, and
A carboxylic acid (5) is obtained. The protecting group of the amino group to be used in the above amino-protecting reaction, the amino acid peptide
Included are amino protecting groups used in chemistry
It For example, urethane type protecting groups such as t-butoxycarbonyl group and benzyloxycarbonyl group, and acyl type protecting groups such as acetyl group, formyl group and trifluoroacetyl group are used. Other known amino protecting groups can also be used
Needless to say.

In the above amino protection, for example, t
-When protected with a butoxycarbonyl group, it is
D-amino acid (6) was dissolved in a mixed solvent of an ether solvent such as tetrahydrofuran and water, and di-t-butyl-di-
After adding 1 to 12 equivalents (preferably 1 to 2 equivalents) of carbonate and triethylamine, the mixture was reacted at room temperature.
You can do it.

In the present invention, piperonylamine is used as a catalyst.
Acylation with rubonic acid (5) or its reactive derivative
As a method for obtaining the compound (3), a known acylation method for synthesizing an amide from a usual carboxylic acid and amine can be applied. When using carboxylic acid (5) in the free state
Is, for example, a method using a known condensing agent such as DCC ( N, N′- dicyclohexylcarbodiimide), carboxylic acid (5) is mixed with a reactive derivative thereof , such as an acid chloride , or a mixture thereof.
Converted to mixed acid anhydride, active ester, active amide, etc.,
Examples of the method include condensation.

The method using a condensing agent such as DCC can be carried out by the following method, for example. That is, Cal
Boronic acid ( 5 ) is dissolved in a halogenated solvent such as chloroform, and 1-hydroxybenzotriazole is added. At this time, 1-hydroxybenzotriazole is 1 to 10 equivalents, preferably 1.1 to 2 equivalents. In addition, piperonylamine and DCC are added. The amount used at this time is
Piperonylamine and DCC are 1 to 10 equivalents, preferably 1.1 to 2 equivalents. Finally the generated dicyclohexylurea may be divided removed by filtration.

The obtained above compound obtained by acylation reaction (3) an amino protecting group removed Hanareka from compound (2)
However, this elimination is related to amino acid and peptide chemistry.
Using a known method of eliminating the amino protecting group
It can be carried out. For example, a method using an acid or a method using catalytic reduction can be applied. In this case, an acid method is preferable because it has a sulfur atom in the molecule.

For example, elimination of the t-butoxycarbonyl group
In the case of conversion, a commonly used acid such as methanesulfonic acid, hydrochloric acid or sulfuric acid can be used. For example, addition of 4N hydrochloric acid in 1,4-dioxane is reacted to the compound (3). The amount of acid at this time is 1 to 100 equivalents, preferably
It is 2 to 5 equivalents. The temperature of the reaction, 0 to 155 ° C., preferably, 60 to 90 ° C.. When the amino protecting group is eliminated with an acid, the resulting compound (2) is obtained in the form of an addition salt of the corresponding acid.

The compound thus obtained (2) The
The Dee to synthesize a compound (1) from an acid addition salt thereof. Zoelens et al., Journal of Organic Chemistry (D. Soerens et. Al.
Org. Chem. , 44 (4), 535-545 (1
Can be synthesized according to the method described in 979))
It

That is, the compound (2) or its acid addition
The salt may be reacted with formalin to cause a cyclization reaction. The amount of formalin used is usually 1 to 1 with respect to the compound ( 2 ).
Use 0 equivalent, preferably 1.5 to 2 equivalents. The reaction solvent used in this process, water and methanol, ethanol, propyl alcohol, it is a mixed solvent of lower alcohol solvents such as hexyl alcohol. The above reaction temperature is 10 to 100 ° C, preferably 50 to 7 ° C.
It is 0 ° C. The reaction time is generally 1 to 30 hours, preferably 20 to 25 hours. Further, as a reaction catalyst, 1 to 10 equivalents of an acid such as hydrochloric acid, sulfuric acid, p-toluenesulfonic acid or the like is used.

The target compound of the present invention thus obtained
Since the product (1) was obtained in the form of an acid addition salt, another acid addition salt was obtained.
When converted into a free base by a known method,
An acid which forms a desired pharmacologically acceptable acid addition salt,
For example, hydrochloric acid, sulfuric acid , inorganic acids such as phosphoric acid , acetic acid, apple
Neutralize with organic acids such as acids, citric acid, tartaric acid, glycolic acid, mandelic acid, maleic acid, p-toluenesulfonic acid
Can be done by

The acid addition salt of the compound (1) obtained is further
Recrystallization, silica gel, if purification is required for
Column chromatography using carriers such as adsorption resins
It can be purified by a known purification method of.

[0035]

The present invention will be described in more detail with reference to Reference Examples and Examples, but the present invention is not limited thereto. In addition, from compound (7) to (R) -N-piperoni
Ru-1,2,3,4-tetrahydrobenzo [b] thieno
Obtained [2,3-c] pyridine-3-carboxamide hydrochloride
The manufacturing reaction formula is as follows.

[Chemical 20]

[0036]

Reference Example 1 A slurry of 500 ml of tap water and 150 g of racemic α-acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid was added to a 4N-sodium hydroxide solution. Was added and dissolved to adjust the final pH to 6.5. 1.0 g of acylase (Amano Pharmaceutical Co., Ltd. 30000 units) and cobalt chloride 6 in this solution
Add 0.072 g of water salt, and add 2 at 38 ° C and pH 6.5.
Stir for 4 hours. L-2-amino-precipitated in the reaction solution
(Ben zo [b] Ji thiophene-3-yl) - crystalline propionic acid is filtered, washed with water, and drying the crystals white L-2-amino - (Ben zo [b] Ji thiophene - 59.7 g of 3-yl) -propionic acid crystals were obtained. This crystal is used as a gas chromatography industry HPLC column Unisil Pack F3-
50A, eluent 35% methanol / 50mM KH ₂ P
As a result of analysis by O ₄ , the purity was 99.95%.

[Α] ^D ₂₅ = 9.17 ° (C 0.6, 0.
1N-HCl), an optical isomer separation column manufactured by Daicel Chemical Co., Ltd., Crown Pack CR (+), and an eluent pH 4.0.
Result of examining the optical purity, the Oh Rukoto with L- body 100% pure product was confirmed by 10% aqueous methanol.

[0038] L-2-amino - (Ben zo [b] Ji Ofe <br/> down-3-yl) - analysis of propionic acid ^{_{IR (ν max, cm -1)}} : 3030,1665,159
On the other hand, concentrated hydrochloric acid was added to about 500 ml of the separated mother liquor to adjust the pH to 3.
It was reduced to 0 and crystallized. After obtaining crystals by filtration, 0.01
The crystals washed with N-HCl are dried to give white, D-α.
73.5 g of -acetamino-α-carboxy-β- (3-benzo [b] thiophen-3-yl) -propionic acid (7) crystals were obtained.

The crystals were analyzed with Unisil Pack F3-50A, a gas chromatography industry HPLC column, and 35% methanol / 50 mM-KH ₂ PO _{4 as} an eluent, and as a result, the purity was 99.91%. Next, Daicel Chemical Industries, Ltd. optical isomer separation column Chiralcel OJ, eluent hexane: 2-
As a result of examining the optical purity with propanol: trifluoroacetic acid 90: 10: 0.1, the D-form was 99.7%.

D-α-acetamino-α-carboxy-
β- (3-benzo [b] thiophen-3-yl) -propionic acid (7) 0.5 g, 5N-methanesulfonic acid 5 m
1 was added, and the mixture was stirred at 100 ° C. for 6 hours and 30 minutes. Replace the water 20 m l was added to room temperature, p in 6N- sodium hydroxide
H was adjusted to 6.5. And the precipitated crystals were collected by filtration and dried, D-2-amino - (Ben zo [b] Ji thiophene-3-
0.41 g of ( yl) -propionic acid (6) was obtained. (Yield 93.0%) D-2-amino - (Ben zo [b] Ji thiophene-3-yl) - analysis of propionic acid IR (ν _max, c
m ^-1 ): 3050, 1670, 1585

[0041]

[Reference Example 2] D-2-amino - (Ben zo [b] Ji Ofe <br/> down-3-yl) - was dissolved propionic acid (6) 53.5 g of tetrahydrofuran 350 ml, water 350 ml, 0
Cool to ° C and di-t-butyl-dicarbonate 6
7.4 g was added. After further adding 41 ml of triethylamine at room temperature and stirring for 4 hours, the solvent was concentrated under reduced pressure and washed with 500 ml of ethyl acetate and 500 ml of 1N hydrochloric acid, and the organic layer was further washed with 100 ml of water and 500 m of saturated saline.
It was washed with 1 and dried over sodium sulfate. The organic layer is dried over magnesium sulfate and concentrated under reduced pressure to dryness to give D-2-
(T-butoxycarbonylamino) - (Ben zo [b] Ji <br/> thiophene-3-yl) - propionic acid (5a) 77.4
g was obtained. (100% yield)

NMR (δ, DMSO-d6): 1.00
~ 1.57 (m, 9H), 3.08 to 3.40 (m, 2
H), 4.21 to 4.52 (m, 1H), 7.12 to
8.09 (m, 5H) IR (ν _max , cm ⁻¹ ): 3440, 2940, 170
0, 1635, 1560

[0043]

EXAMPLE 1 D-2-(t-butoxycarbonylamino) - (Ben zo [b] Ji thiophene-3-yl) - propionic acid (5a) 58.7 g, 1-hydroxybenzotriazole - le 24. 7 g, piperonylamine (4) 19.4
Dissolve ml in 820 ml chloroform and DC at 0 ° C.
C (N, N'- dicyclohexylcarbodiimide ) 4
1.4g was added and it stirred at room temperature for 1 hour. The precipitated dicyclohexylurea was removed by filtration. It was washed with 300 ml each of a saturated sodium hydrogen carbonate solution, a 10% citric acid solution and a saturated saline solution, and dried with sodium sulfate. The solvent was distilled off so that the amount of the solvent was about 300 ml, 500 ml of ether was added, and the mixture was left standing for 16 hours. The precipitated crystals were collected by filtration, (R)-N-piperonyl-2-(t-butoxycarbonylamino) - (Ben zo [b] thiophene -3
55.4 g (yield 67%) of (-yl) -propionylamide (3a) was obtained.

NMR (δ, CDCl ₃ ): 1.36
(S, 9H), 3.29 (d, 2H, J = 7.5H
z), 4.13 (d, 2H, J = 7.5 Hz), 4.4
3 (q, 1H, J = 7.5 Hz), 5.97 (s, 2
H), 6.00-6.30 (m, 1H), 6.30-
6.70 (m, 3H), 7.10 to 7.90 (m, 5
H) IR (ν _max , cm ^-1 ): 3300, 1680, 164
5, 1565, 1520

[0045]

EXAMPLE 2 (R)-N-piperonyl-2-(t-butoxycarbonylamino) - (Ben zo [b] Ji thiophene -
63.7 g of 3-yl) -propionylamide (3a) was added to 500 ml of dioxane. 4N at room temperature
142 ml of a hydrochloric acid dioxane solution was added, and the mixture was stirred at 80 ° C for 1 hour. When the organic solvent is concentrated under reduced pressure to 150 ml at 60 ° C, crystals start to precipitate. The crystals are collected by filtration,
(R)-N-piperonyl -2-amino - (Ben zo [b]
Chi thiophene-3-yl) - propionyl hydrochloride
(2a) 52.3g was obtained (yield 95%).

NMR (δ, DMSO-d6): 3.20
Up to 3.40 (m, 2H), 4.00 to 4.20 (m, 3
H), 5.90 (s, 2H), 6.61 (dd, 1H,
J = 7.9 Hz, J = 1.3 Hz), 6.72 (d, 1
H, J = 1.3 Hz), 6.78 (d, 1H, J = 7.
9 Hz), 7.30 to 8.10 (m, 5H), 8.51
(Bs, 3H), 9.04 (m, 1H) IR (ν _max , cm ⁻¹ ): 3250, 2970, 166
0,1550

[0047]

Example 3 (R) -N-piperonyl-2-amino-
(Ben zo [b] Ji thiophene-3-yl) - propionyl hydrochloride (2a) 10.0 g of methanol 50m
1 and dissolved in 50 ml of water, and 32 ml of 5N hydrochloric acid was added.
35% formalin 6.55 ml was added to 70-90 ° C.
And stirred for 7 hours. Upon cooling to 0 to 10 ° C, crystals were precipitated and collected by filtration. Recrystallized from methanol-ether,
9.67 g of (R) -N-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride (1a) was obtained (yield 9
4%).

NMR (δ, CD ₃ OD): 2.33 to
4.12 (m, 5H), 5.93 (s, 2H), 6.7
7 to 8.07 (m, 9H) IR (ν _max , cm ⁻¹ ): 3250, 2900, 168
5, 1435, 760

[0049]

In Reference Example 3] Example 1, D-2-amino Reference Example 2 - (Ben zo [b] Ji thiophene-3-yl) - L-2-amino in place of propionic acid 53.5 g - (Ben Zo
[B] Ji thiophene-3-yl) - propionic acid 53.5
(S) -N-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride (9.11 g) was obtained in exactly the same manner except that g was used. (However, only the final yield of 89% is shown).

(S) -N-piperonyl-1,2,3,4
Analysis result of tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride NMR (δ, CD ₃ OD): 2.33 to 4.12 (m,
5H), 5.93 (s, 2H), 6.77 to 8.07.
(M, 9H) IR (ν _max , cm ⁻¹ ): 3250, 2900, 168
5, 1435, 760

[0051]

Next, (R) -N-piperonyl-1,
2,3,4-Tetrahydrobenzo [b] thieno [2,3
-C] pyridine-3-carboxamide hydrochloride is (S) -
The following test shows that it is superior to N-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride.

1. 2 weeks oral repeated dose toxicity test Crj: in Wistar male rats, N- piperonyl -
Of 1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride
(R) -body and (S) -body are 100, 200,
300 mg / kg each of 5 animals was continuously administered for 2 weeks, and the effect on the liver was observed. The test results are shown in Table 1 .

[0053]

[Table 1]

From the results shown in Table 1, the (S) -form is 100 mg.
/ Abnormal liver enlargement or the like Ru seen in kg, while the present invention
(R) -body was 100 mg / kg with no liver abnormalities.
The (R) -form is less toxic to the liver than the (S) -form, and is considered to be a safer compound.

[0055]2.Anti-anxiety testExamination Using Wistar male rats (6 weeks old), Vogel.
J. Earl and Bear. Bee, Clodie. Dee.
E, Psycho Pharmacologia (Vogel JR,
Beer B. , And Clody D .; E. , Ps
ychopharmacologia) 1-7,21
Water Conflict based on (1971)
Kuto test (Water lick confli
cttest), N-piperonyl-1,2,
3,4-Tetrahydrobenzo [b] thieno [2,3-
c] pyridine-3-carboxamide hydrochloride(R)-Body
as well as(S)-The anxiolytic effect of the bodyMeasuredIt was

In this test, dehydrated rats are allowed to drink water.
The drug was administered 4 to 5 hours later, and the test was started after the treatment time. The number of shocks is the number of electric shocks that a rat receives only water for 5 minutes, and indicates whether or not drinking water suppresses the conflict (anxiety) of receiving an electric shock. In other words, the fact that the number of the shock increases, which means that the anti-anxiety effect was enhanced. Table 2 shows the values when the drug-non-administered rats were set to 100 .

[0057]

[Table 2]

From the results in Table 2, it is clear that the (R) -form is
(S) - body strong significant anxiolytic effects in comparison, to medicinal manner (R) - body (S) - indicating that the significantly superior to the body.

3. Serotonin _1A binding No試Experiment (1) preparation method male Sprague-Dawley rats of serotonin _1A receptor membrane (7 weeks old,
After decapitating (Charles River), the cerebrum was immediately removed and the hippocampus was extracted under ice cooling. Tissue is 50 mM Tris-
The mixture was homogenized with HCl buffer (pH 7.4) and then centrifuged (48,000 xg, 15 min). The precipitate was resuspended in the above buffer and incubated at 30 ° C. for 20 minutes (decomposition of endogenous 5-HT), 4
It was centrifuged at 8,000 xg for 15 minutes. The obtained sediment is b
indicing buffer (50 mM Tris-H
Cl, 2.5 mM MgCl ₂ pH 7.4) was suspended to obtain a membrane preparation.

(2) Experiments and Results A membrane preparation was used in a total volume of 1 ml to give 0.5 nM ³ H-8-OH-.
Incubated with DPAT and 10 nM pargyline (MAO inhibitor) at 30 ° C. for 30 minutes. The measurement of non-specific binding while changing the drug concentration was performed in the presence of 10 μM serotonin, and the value obtained by subtracting this value from the measurement value in the absence of serotonin was used for specific 3H-8-OH-D.
PAT binding was used. After the completion of the incubation, the reaction solution was suction filtered with a glass fiber filter paper (Whatman GF / C), and the filter paper was mixed with 4 ml of binding buffer to 3 times.
After washing twice, it was transferred to a vial bottle and a scintillator was added to measure radioactivity. The results are shown in Table 3 .

[0061]

[Table 3]

From the results shown in Table 3, the (R) -form was more selectively bound to serotonin _1A than the (S) -form. As shown in Test Example 2, anxiolytic action (R) - body (S) - stronger than body and, in <br/> binding capacity stronger this serotonin _1A, mechanism of action to It is suggested.

[0063] or more Ri by utility of Test Examples 1, 2 and 3 results (R)-N-piperonyl-1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine -
3-carboxamide hydrochloride is (S) -N-piperonyl-
It is clear that it is superior to 1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride.

As described above, (R) -N-piperonyl-
1,2,3,4-tetrahydrobenzo [b] thieno [2,3-c] pyridine-3-carboxamide hydrochloride is (S) -N-piperonyl-1,2,3,4-tetrahydrobenzo [b] Stronger anxiolytic effect, which is more effective than thieno [2,3-c] pyridine-3-carboxamide hydrochloride ,
Serotonin _1A binding ability, which is considered to be the main mechanism of its efficacy
Is significantly stronger and has less hepatotoxicity. Therefore,
The optically active (R) -N-piperonyl-1,2,
3,4-Tetrahydrobenzo [b] thieno [2,3-
c] Pyridine-3-carboxamide or acid addition salt thereof
When administered to humans or animals in free form or in the form of its salt,
Since it has a strong anxiolytic effect and low toxicity,
Suitable as a psychotropic drug.

Claims (2)

[Claims] 1. The following compound: An optically active DN-piperonyl-1,2,
3,4-Tetrahydro-benzo [b] thieno [2,3-
c] Pyridine-3-carbamide, a drug containing the same, and an acid addition salt thereof. 2. The following compound: The L-form of the racemic form of the non-natural acylamino acid represented by is deacylated with an acylase to give the following compound: An unnatural L-amino acid represented by the following compound and the following compound The following compound, characterized in that it is separated into a non-natural acyl-D-amino acid represented by An optically active DN-piperonyl-1,2,
3,4-Tetrahydro-benzo [b] thieno [2,3-
c] A method for producing pyridine-3-carboxamide.