JPH0698917A - Ultrasonic cell membrane matching vibration treatment of malignant tumor - Google Patents

Abstract

PURPOSE:To prohibit the growth of malignant tumors and eventually to reduce and kill the malignant tumors by regarding the malignant tumors as the assemblage of individual elastic bodies each having a membrane structure and selectively irradiating the peripheral parts of the tumor cells with ultrasonic waves near the resonance frequencies possessed by the tumor cells. CONSTITUTION:This treatment method prohibits oxygen activity and kills the cancer cells by irradiating the lesions with the ultrasonic waves of the frequencies matched with the frequencies of the cancer cells, thereby losing the transmittability of the cell membranes of the cancer cells. More specifically, the three-dimensional ultrasonic irradiation treatment method of the composite frequencies using the composite frequencies of low frequencies and high frequencies is used. Specimens are first taken out of a part of the cancer cells and the characteristics of the cells are measured by a resonance frequency analyzer and thereafter, the frequencies are determined at the time of the treatment of a patient 6 of the progressive cancer transferred with the cancers 7 over the entire part of the body. The patient 6 is then put into a bath tub 5 contg. a physiological salt soln. 9 kept at about 37 deg.C and is irradiated with the composite frequencies generated by sound wave oscillating sources 8... for about two to three hours, by which the treatment is executed.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a more effective ultrasonic treatment method for malignant tumors, for example, in the medical field, and more specifically to the elasticity of a membrane structure having different natural frequencies for cells of each part of the human body. As a body group, the present invention relates to an ultrasonic treatment method and an ultrasonic health promotion method that make use of the unique frequency characteristics of each cell group.

[0002]

2. Description of the Related Art Conventionally, as a method of treating a malignant tumor using ultrasonic waves, that is, cancer, hyperthermia using ultrasonic waves has been the main focus. It utilizes the heat generated when the energy emitted from the oscillator of the ultrasonic oscillator is attenuated at the tumor part in the body. They mainly focus on the difference in heat resistance between normal cells and cancer cells in the human body against high temperatures, and apply the fact that cancer cells have slightly lower thermophysical properties than normal cells.

However, in the ultrasonic treatment methods up to now, only a slight difference in heat resistance between normal cells and cancer cells has been utilized because it is caught only by the hyperthermic effect.

[0004]

The problem to be solved by the present invention is to use ultrasonic waves to minimize the effect of cancer cells formed in the human body on normal cells, and to selectively transform cancerous cells. It is intended to provide a method for efficiently inhibiting at least growth and killing only the killed cells.

[0005]

The first invention of the present application is to focus on the specificity of cancer cells. Cancer cells have specificity in the state of the cell membrane as compared to normal cells, and differences in cell membrane strength, cell size, and elasticity can be found. As a result, when the cells of the human body are regarded as elastic bodies, the natural frequencies of the normal cells and the cancer cells, that is, the resonance frequencies of the normal cells and the cancer cells when stimulated by sound pressure from the outside are given. Will be different. It is known that the elastic body vibrates most effectively when it receives vibration energy from the outside in the vicinity of its resonance frequency. Therefore, when a cancer cell receives vibrational energy from the outside in the vicinity of the resonance frequency, its cell membrane moves most, and depending on the intensity of the energy, it destroys the physiological action of the lipid molecule forming the membrane and permeates the cell membrane. It becomes possible to lose the activity and the activity of the enzyme bound to the membrane. Furthermore, if the resonance energy given to the cancer cells is increased, the cell membrane can be destroyed. Further, since the attenuation of energy is maximized near the resonance frequency, it is expected that the combined use of the thermal effect will be highly efficient.

In specific treatment, first, cancer cells in a cancerous part of a patient are sampled, and at the same time, peripheral normal cells are sampled, and these samples are sampled by using a reflectance measuring device or the like. It starts by measuring the natural frequency of each cell, that is, the resonance frequency. After measuring the resonance frequency of the cancer cells and normal cells in the surrounding area and normal cells on the irradiation path when performing ultrasonic treatment, the ultrasonic radiation energy intensity and normal cells for obtaining the therapeutic effect at the resonance frequency of the cancer cells Experimentally and theoretically determine the safety limit for. The frequency thus determined becomes the frequency for treating the corresponding patient. When the cancer has metastasized and exists at multiple sites, it is natural that different frequencies are used for treatment. When a sample cannot be taken directly from the patient, the oscillation frequency of the cancer cells can be estimated from the frequency response characteristics of the affected part when a pulsed sound wave signal is applied to the affected part from the outside.

The method of treatment is the same as in the ordinary ultrasonic treatment method, in which an ultrasonic transducer is used as a medium with degassed liquid such as water, liquid paraffin, olive oil, castor oil, etc. When the irradiation is performed in close contact, when the patient is soaked in a treatment device such as a bath filled with degassed liquid for irradiation, or when the patient is directly irradiated on the affected area during surgery,
There are methods such as inserting an ultrasonic transducer attached to the tip of the cable into the oral cavity for irradiation.

The second invention of the present application expands the concept of the first invention and provides a similar treatment method for biological organisms such as bacteria. Even for bacteria etc. in liquid, the resonance frequency as an elastic body of cells and bacteria in liquid can be determined by utilizing the characteristics of microscopic objects as an elastic body and the characteristics of physiological action of surface membrane. This is a method of selectively killing only the target group of interest by irradiating the corresponding ultrasonic waves.

The third invention of the present application is to stimulate the cell membrane by irradiating the human body with ultrasonic vibration of sufficiently weak energy that does not harm normal cells at the resonance frequency of each normal cell of the human body. To increase the physiological activity of the cell membrane.

[0010]

In the present invention, not only is ultrasonic irradiation used as a conventional thermal effect, but growth inhibition and death are achieved mainly by giving maximum kinetic energy to the cell membrane of cancer cells. By utilizing the difference in resonance frequency Q between normal cells and cancer cells as the body and selecting and using ultrasonic waves with a frequency that matches the individual cancer cells of each patient, the target treatment is efficiently performed. be able to.
In addition, because it is possible to treat with relatively weak irradiation energy,
The so-called cavitation phenomenon can be avoided, and the effect can be further improved by projecting a focused ultrasonic wave into the deep part of the human body. When the cells are sealed in normal cells and irradiated with a weak ultrasonic wave near their resonance frequency, the cell membrane is moderately stimulated and the metabolism of each cell is promoted.

[0011]

EXAMPLE FIG. 1 is a model diagram of the intracellular membrane structure.
By irradiating the affected area with ultrasonic waves having a frequency matched with the cancer cells, the permeability of the cell membrane of the cancer cells is lost, the enzyme activity is inhibited, and the cancer cells are killed. 1,
2 is a protein molecule, 1 is a hydrophilic part, 2 is a hydrophobic part, 3, 4
Is a lipid molecule of the cell membrane, 3 is a hydrophilic part, and 4 is a hydrophobic part.

[0012]

INDUSTRIAL APPLICABILITY The present invention focuses on the frequency matching with cancer cells as an elastic body, which has not been a problem so far in ultrasonic treatment of cancer, and further, By placing the therapeutic mechanism of the above in the excessive vibration of the cell membrane of cancer and the obstruction of permeability due to movement, we provided a safer, more efficient, and more repeatable treatment method. Although the structure of the human body is complicated and it is difficult to apply the present invention to the treatment of all cancers, the frequency and irradiation in ultrasonic treatment will increase as the actual treatment results of many cancer patients accumulate. The strength relationship, the relationship with the permeability inhibition of the cancer cell membrane, the irradiation method, etc. will be clarified, which will bring further progress in the field of ultrasonic therapy.

[0013]

[Brief description of drawings]

FIG. 1 Model of intracellular membrane structure

[Explanation of symbols]

 1 protein molecule hydrophilic part 2 protein molecule hydrophobic part 3 cell membrane lipid molecule hydrophilic part 4 cell membrane lipid molecule hydrophobic part

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[Procedure amendment]

[Submission date] October 2, 1992

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be amended] Claims

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[Claims]

[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0006

[Correction method] Change

[Correction content]

[0006] In specific treatment, first, cancer cells of a patient's cancer affected area are collected, and at the same time, peripheral normal cells are collected, and these specimens are sampled by using a reflectance measuring device or the like. It starts by measuring the natural frequency of each cell, that is, the resonance frequency. After measuring the resonance frequency of the cancer cells and normal cells in the surrounding area and normal cells on the irradiation path when performing ultrasonic treatment, the ultrasonic radiation energy intensity and normal cells for obtaining the therapeutic effect at the resonance frequency of the cancer cells Experimentally and theoretically determine the safety limit for. The frequency thus determined becomes the frequency for treating the corresponding patient. When the cancer has metastasized and exists at multiple sites, it is natural that different frequencies are used for treatment. When a sample cannot be directly collected from the patient, the resonance frequency of the cancer cells can be estimated from the frequency response characteristics of the affected part when a pulsed sound wave signal is externally applied to the affected part. In addition, the fourth issue of the present application
The method by Ming is not a relatively stable interphase of cancer cells.
A few tens to several hundreds of kilohertz, aiming for an unstable mitotic period
Single oscillation of sound waves of relatively low frequency such as
Not at the source, but at least in two dimensions, preferably three
By irradiating the affected area or the whole body from the dimensional direction
Therefore, it destroys the cell membrane of the cancer cell in the mitotic phase.
Sound waves are emitted by combining low-frequency and high-frequency sound waves.
It is more preferable to do so. Combined frequency irradiation is also available
Can be spread. Low frequency sound waves are attenuated in the body
A few, effective to reach deep inside the body, three-dimensional
Directional irradiation causes cancer cells to undergo three-dimensional movement, usually
Only at the time of division of cancer cells without damaging cells
It gives a mitotic inhibitory effect on the growth inhibition, shrinkage and death of cancer cells.
To do. By attacking cancer cells in the mitotic phase,
It is possible to treat with weak energy irradiation,
The safety aspect is further improved. In any case
Always treat patients with light before, during, and after irradiation.
Sound wave irradiation is performed while monitoring the changes in
The safety aspect is always managed so as not to have an adverse effect on the inside. Well
Of nutritional supplements and anti-cancer drugs to patients undergoing sonic irradiation
Can be used in combination.

[Procedure 3]

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[Correction method] Change

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As in the case of the usual ultrasonic treatment method, the ultrasonic wave is used on the body surface of the projection site by using an ultrasonic transducer as a medium of degassed liquid such as water, liquid paraffin, olive oil, castor oil, etc. When the irradiation is performed in close contact, when the patient is soaked in a treatment device such as a bath filled with degassed liquid for irradiation, or when the patient is directly irradiated on the affected area during surgery,
There are methods such as inserting an ultrasonic transducer attached to the tip of the cable into the oral cavity for irradiation. Ultrasonic irradiation
Energy is below the range used for conventional ultrasound therapy
Used at high energy, especially slightly off resonance
Treatment method using low frequency band sets patient to temperature around body temperature
Put it in the bathtub containing the physiological saline, etc.
By applying moderately weak sound waves repeatedly for a long time,
And aim at the relatively fragile stage of cell division in cancer cells
Therapies that inhibit cancer and are expected to be effective in some advanced cancers
Is.

[Procedure amendment 4]

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[Correction target item name] 0010

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[0010]

In the present invention, not only is ultrasonic irradiation used as a conventional thermal effect, but growth inhibition and death are achieved mainly by giving maximum kinetic energy to the cell membrane of cancer cells. By utilizing the difference in resonance frequency Q between normal cells and cancer cells as the body and selecting and using ultrasonic waves with a frequency that matches the individual cancer cells of each patient, the target treatment is efficiently performed. be able to. In addition, since the treatment can be performed with relatively weak irradiation energy, so-called cavitation phenomenon can be avoided, and the effect is further improved by projecting as a focused ultrasonic wave on the deep part of the human body. Furthermore, the low frequency band
With relatively weak irradiation energy, at least two-dimensional
From the direction, if the whole body is repeatedly irradiated for a long time, comparison
Unstable, impairs the cell membrane during the cell division of cancer cells
Efficacy of cancer cell growth reduction, regression, and death due to mitotic inhibition
Can be expected . When normal cells are irradiated with weak ultrasonic waves near their resonance frequency, the cell membrane is moderately stimulated and the metabolism of each cell is promoted.

[Procedure Amendment 5]

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[Correction method] Change

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[0011]

EXAMPLES FIG. 1 is a model diagram of the intracellular membrane structure. By irradiating the affected area with ultrasonic waves having a frequency matched with the cancer cells, the permeability of the cell membrane of the cancer cells is lost, the enzyme activity is inhibited, and the cancer cells are killed.
1, 2 are protein molecules, 1 is a hydrophilic part, 2 is a hydrophobic part,
3 and 4 are lipid molecules of the cell membrane, 3 is a hydrophilic part and 4 is a hydrophobic part. Figure 2 uses a combination of low and high frequencies
3D sonication with multiple frequencies. Cancer in the whole body
Samples from some cancer cells for patients with metastatic advanced cancer
The resonance frequency analyzer using a crystal unit.
Then, the characteristics of the cells are measured and other specimens are taken out.
Irradiation is focused on low frequencies for cancer in difficult areas.
The temperature of the saline solution in the bathtub is about 37 degrees C, and the irradiation time is
Start from about 20 minutes and check the patient's blood pressure and other conditions.
While increasing, and finally 2 to 3 hours / time
It The operating frequency is 100 Hz to 10 kHz on the low frequency side.
Band, high frequency side uses 1MHz to 10MHz band
It Irradiation energy starts from about 0.1 W / cm
While looking at the condition of the person, a range that does not impair the normal part of the human body
It increases sequentially in the circle. Patient should be a nutritional supplement before sonication
, Etc., and the anticancer drug was administered after the sonication.
It Patients are regularly treated by tomography of the affected area
Continue treatment while confirming and evaluating the effect. Figure 3 shows the liver
This is an example of treatment for a patient with spleen cancer. Sample of patient's cancer affected area
The treatment frequency and radiant intensity are determined and the treatment is performed. Radiation time
And radiant intensity start from below 50% of the safety setting line
Next, increase while watching the patient's condition.

[Procedure correction 6]

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FIG. 2 is an example diagram of ultrasonic whole body irradiation treatment in a bath .

[Procedure Amendment 7]

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FIG. 3 is a diagram showing an example of treatment of local cancer .

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[Explanation of symbols] 1 protein molecule hydrophilic part 2 protein molecule hydrophobic part 3 lipid molecule of cell membrane hydrophilic part 4 lipid molecule of cell membrane hydrophobic part 5 bath 6 patient to be treated 7 cancer affected area 8 sound wave source 9 saline 10 patient's Trampling grid 11 floor 12 bag containing deaerated water

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[Figure 3]

[Procedure amendment]

[Submission date] October 8, 1992

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be corrected] Claim 4

[Correction method] Change

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[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0006

[Correction method] Change

[Correction content]

[0006] In specific treatment, first, cancer cells in a cancer affected area of a patient are collected, and at the same time, normal cells around the cancer cells are collected, and these specimens are sampled by using a reflectance measuring device or the like. It starts by measuring the natural frequency of each cell, that is, the resonance frequency. After measuring the resonance frequency of the cancer cells and normal cells in the surrounding area and normal cells on the irradiation path when performing ultrasonic treatment, the ultrasonic radiation energy intensity and normal cells for obtaining the therapeutic effect at the resonance frequency of the cancer cells Experimentally and theoretically determine the safety limit for. The frequency thus determined becomes the frequency for treating the corresponding patient. When the cancer has metastasized and exists at multiple sites, it is natural that different frequencies are used for treatment. When a sample cannot be directly collected from the patient, the resonance frequency of the cancer cells can be estimated from the frequency response characteristics of the affected part when a pulsed sound wave signal is externally applied to the affected part. The method according to the fourth invention of the present application aims at an unstable mitotic phase of cancer cells, rather than at a relatively stable intermediate phase, and aims at several tens to several hundreds of kilohertz
Appropriate time a relatively low frequency of the acoustic wave such, rather than a single oscillation source, at least opposite the two-dimensional direction, preferably from the three-dimensional directions, by irradiating the affected part or whole body, cell membrane mitotic cancer cells To destroy. It is more preferable to irradiate sound waves by irradiating sound waves of low frequency and high frequency in combination. Multiple frequency irradiation can further expand the range of coverage. Low-frequency sound waves are not attenuated in the body and effectively reach deep inside the body. Irradiation from three-dimensional directions causes cancer cells to undergo three-dimensional compression and expansion. , It gives a mitotic inhibitory effect only at the time of division of cancer cells without damaging normal cells, and inhibits growth, shrinkage, and death of cancer cells. By attacking cancer cells in the mitotic phase, it becomes possible to treat with relatively weak energy irradiation, and the safety aspect is further improved. In any case, the treatment according to the present invention is always carried out while monitoring changes in the patient's pre-irradiation, during irradiation, and post-irradiation conditions, and safety is always controlled so that sonic irradiation does not adversely affect the body. It In addition, administration of a nutritional supplement, administration of an anti-cancer drug, etc. can be used in combination for a patient who is subjected to ultrasonic wave irradiation.

[Procedure 3]

[Document name to be amended] Statement

[Correction target item name] 0010

[Correction method] Change

[Correction content]

In the present invention, not only is ultrasonic irradiation used as a conventional thermal effect, but mainly by giving maximum kinetic energy to the cell membrane of cancer cells,
In order to inhibit growth and kill, and by utilizing the difference in resonance frequency Q between normal cells and cancer cells as elastic bodies, select and use an ultrasonic wave with a frequency that matches the individual cancer cells of each patient The target treatment can be performed efficiently. In addition, since the treatment can be performed with relatively weak irradiation energy, so-called capitation phenomenon can be avoided, and the effect is further improved by projecting as a focused ultrasonic wave on the deep part of the human body. Further, using a low frequency band, a relatively weak irradiation energy, to at least the relative
From the two-dimensional direction. If the whole body is repeatedly irradiated for a long time, the cell membrane is damaged during the cell division stage of the cancer cell, which is relatively unstable, and the growth inhibition and regression of the cancer cell due to the inhibition of division occur.
A killing effect can also be expected. When normal cells are irradiated with weak ultrasonic waves near their resonance frequency, the cell membrane is moderately stimulated and the metabolism of each cell is promoted.

Claims (3)

[Claims] 1. A malignant tumor is regarded as an aggregate of individual elastic bodies having a membrane structure, and by utilizing the difference in cell membrane strength and natural frequency from surrounding normal cells, the tumor corresponding to the peripheral part of the tumor cell is identified. By selectively irradiating ultrasonic waves in the vicinity of the resonance frequency of the cells, at least the cell membrane of the relevant tumor cells is denatured, and further, the cell membrane is destroyed, at least the growth of malignant tumors is blocked, and further reduction and death are performed. A method for treating a malignant tumor, which comprises: 2. Various natural frequencies can be obtained by selectively irradiating a micro organism having a property of dying when the outer membrane is destroyed or denatured, with a sound wave near the natural frequency that the micro organism has as an elastic body. A method of dying a small organism, which comprises selectively erasing a small organism group having the same natural frequency from a group of small organisms having a. 3. A cell membrane of each part of a human body by selectively irradiating each part with ultrasonic waves in the vicinity of a natural frequency as an elastic body of a normal cell of each part of the human body A method for promoting health, which is characterized by stimulating the cells appropriately to increase the activity of the cell membrane and promote metabolism.