JPH0665061A - Additive to cleaning solution and surgical solution - Google Patents

Abstract

PURPOSE: To provide a soln. which is used as a washing soln. and an additive to a surgical soln. in the anterior and posterior chambers of the eye and is further used for other surgical operation and treatment means, such as the preserving media (preserving fluid) for the cornea. CONSTITUTION: This washing liquid for the inside of the eye and the additive for the surgical procedure soln. are supplied to the anterior and posterior chambers of the eye accompanied with a protective film during the surgical optical requiring washing. The additive for the soln. comprises an equiv. salt soln. contg. dextrose added with chondroitin sulfate. The additive may be added with other chemical compsns. as well.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention is used as an additive for irrigation solutions and surgical solutions in the anterior and posterior chambers of the eye.
Furthermore, this additive can be used as a solution for other surgical operations and medical procedures such as a preservation medium (preservation solution) for cornea.

[0002]

EXAMPLES Generally, there are two types of intraocular irrigation solutions used for intraocular surgery. These two washing solutions are balanced salt solution and BSS Plus. BSS is a balanced salt solution with a buffering system of sodium acetate and sodium citrate. BS
S Plus consists of a bicarbonate buffer system and a balanced salt solution that provides glucose with an energy source and permeability. The added components and oxidized glutathione are reduced by ocular cells and function as antioxidants. In addition, lactate Ringer's solution is BSS and B
Used when SS Plus is unavailable.

[0003] The present invention also relates to a surgical treatment solution or additive to the irrigation solution that is supplied to the anterior and posterior chambers of the eye that protects the eye during surgical procedures that require irrigation.

This additive protects the corneal endothelium particularly significantly in the anterior chamber surgical procedure.

The corneal endothelium as well as other anterior and posterior chamber tissues are in direct contact with additives in solution.

According to one embodiment of the invention, the additive contains the following composition:

1. A protective coating agent which is a biodegradable substance usually found in the cornea of a human, is naturally produced, and contains a very small amount of glycosaminoglycan such as chondroitin sulfate.

2. An effective antioxidant such as 2-mercaptoethanol that can be used for both oxides and reductants by human corneal endothelial cells. 2-Mercaptoethanol is usually used intracellularly as a (transport) carrier for cystine. Cystine, like glutathione, may be a limiting amino acid that synthesizes proteins. 2-Mercaptoethanol increases the intracellular accumulation of glutathione and assists the film formation and repair of the connected composite cells.

3. It is an energy source such as dextrose (D-glucose) and is glycolytic, that is, ATP.
It is the starting material of the central metabolic pathway that takes in the chemical energy of (adenosine triphosphate).

4. An additional source of energy, such as pyruvate, is supplied with additional biosynthetic substances that are essential for eye cells after surgical trauma.

5.2-The amino acid cystine present in 2-mercaptoethanol, which is supplied so that endothelial cells of the cornea can constantly utilize the cystine in the additive to keep the organ at the injury site healthy. Is supplied to maintain glutathione levels during perfusion and cystine in cells.

6. A bicarbonate buffer system (bicarbonate buffer system) that is important for intracellular tissue fluids to help regulate intracellular pH, flow tissue fluids, and create a membrane voltage between different endothelial cells. A basic solution of a balanced salt solution or a wash solution with an additive consisting of a lactate Ringer solution containing the essential 5 ionic components, or a surgical treatment solution.

The characteristics and one important aspect of the additive according to the present invention are that it protects the anterior and posterior chambers of the cornea in the treatment by surgical operation, and maintains homeostasis (homeostasis) after injury of surgical trauma. A) and supply the metabolic substrates needed for wound healing.

As a characteristic and another important aspect of this additive, when it is added to a cleaning solution, it is comprehensively applied to the following different medical uses.

1. 1. Eye drops that cleanse the eyes and impart wetness and lubricity; Liquid for burns; 3. 3. Surgical solutions for all surgical procedures that require irrigation solutions; 4. Excipients for pharmaceuticals in the ophthalmological field, such as drops that impart wetting and lubricating properties; Cataract therapeutic agent; 6. 7. Eye drops; 7. 7. Additives for corneal storage media; 9. Orthopedic areas such as histoscopy, urology areas such as cystoscopy, neurosurgery areas, general areas including obstetrics and gynecological areas (OBGYN) including artificial insemination; 9. Liquid medicine for healing (washing) wounds (pain); or 10. Ulcer Remedies Additive solutions added to the lavage solution or surgical treatment solution were 1-100 for each 500 parts by volume of a commercially available effective balanced salt solution (BSS) sold by Iolab or Alcon. Used in volumetric concentrations.

[0016] Preferably, the additive concentrate is from Table A
One or more components of Table A Ingredient Range Specific Ingredients Glycosaminoglycan 0.001-550mg / ml Chondroitin Sulfate Antioxidant 0-10mM 2-Mercaptoethanol Energy Source 0-20mM Dextrose (D-Glucose) 0-20mM Pyruvate Amino Acid 0-10mM Cystine Buffering agent 0-100 mM bicarbonate solution (bicarbonate solution) The formulation is a standard concentration (state) of the balanced salt solution with a total volume of 1-50 ml or the additive prepared with purified water.

As shown in Table B, the additives are 500 m
It is also possible to add 1 balanced salt solution. Therefore, the additive in this wash solution uses the above concentration ratios in all other volumes. Table B 50 ml additive prepared from balanced salt solution (BSS) medium: Chondroitin sulfate 44.00 mg / ml 2-mercaptoethanol 5.50 mM dextrose (D-glucose) 55.00 mM pyruvate 11.00 mM cystine 33.00 mM bicarbonate solution ( Bicarbonate solution) 275.00 mM balanced salt solution (BSS) medium: sodium chlorate 0.64% potassium chlorate 0.075% calcium chloride 0.048% magnesium chloride 0.030% sodium acetate 0.39% sodium citrate 0.17% or distilled water for injection is added. And then prepared. Table C shows the final concentrations of the additives after suitable dilution with the balanced salt solution. Table C Final Concentration Component Final Concentration Approximate Range of Final Concentration Chondroitin Sulfate 4.00mg / ml (0-100mg / ml) 2-Mercaptoethanol 0.50mM (0-2mM) Dextrose (D-glucose) 0.92mg / ml (0-20mM) ) Pyruvic acid 1.00 mM (0-20 mM) Cystin 3.00 mM (0-10 mM) Bicarbonate solution (bicarbonate solution) 25.00 mM (0-100 mM) Therefore, this additive is present at this concentration at all other volumes. Limited to ratio. A concentrate of a GROUPING additive is composed of one or more of the following balanced salt or similar solution components (lyophilized).

A. Glycosaminoglycan: 1.0 mg /
ml to 550 mg / ml. 1. Chondroitin sulfate 2. Dermatan sulfate 3. Heparan sulfate 4. Heparin sulfate 5. Keratin sulfate 6. Hyaluronic acid b. Antioxidant 1. Ascorbic acid: 1 mM-20 mM 2. Glutathione: 10 μg / ml-500 mg / ml 3. Dl-α-tocopherol: 0.01 mg / ml-
1 mg / ml 4.2-mercaptoethanol: 0.1 mM to 8 mM c. Glycoproteins that cause cell adhesion and migration: Laminin is a huge glycoprotein with a molecular weight of about 1,000,000 daltons. Each laminin molecule has 200,
It consists of three B-chains of 000 Daltons and one A-chain of 400,000 Daltons, and has an asymmetric (asymmetric) crossing shape. This chain is held by a disulfide bond. The A single chain exists at the binding position of heparin sulfate. The B chain is present at the binding position of type IV collagen. The intersection of the three B chains is the cell-binding locus. Laminin promotes attachment, cytoplasmic diffusion and dispersal growth, and supplies cells that are physiologically compatible with the extracellular stroma.

Laminin: 1 μg / ml-300 mg / ml 2. Fibronectin is a glycoprotein associated with the extracellular stroma, and each subunit of 220,000 daltons is composed of two disulfide bonds. Fibronectin has a potential for correlating high molecular weight compounds, including collagen, glycosaminoglucans, and several cell surfaces associated with receptors. Fibronectin promotes the binding and attachment of human corneal endothelial cells, epithelial cells, and fibroblasts.

Fibronectin: 100 ng / ml-1
00 mg / ml d. Cell growth factors or growth cofactors: Fibroblast growth factor (FGF) is an acidic or basic single-chain polypeptide isolated and purified from the pituitary gland, human fibronectin (hFGF), or bovine fibronectin (bFGF). The molecular weight is
It is from 14K to 16K. This factor is shown to be a factor that significantly promotes mitosis in cells of tissues including neuroectodermal and mesoderm in vitro.

This also includes the FGF basic peptide consisting of (1-24) artificially synthesized in vitro. : Pro (proline) -Ala (alanine) -Le
u (leucine) -Pro (proline) -Glu (glutamic acid) -Asp (aspartic acid) -Gly (glycine) -Gly (glycine) -Ser (serine) -Gly
(Glycine) -Ala (alanine) -Phe (feralanine) -Pro (proline) -Pro (proline)-
Gly (glycine) -His (histidine) -Phe
(Phenylalanine) -Lys (lysine) -Asp (aspartic acid) -Pro (proline) -Lys (lysine) -Arg (arginine) -Leu (leucine) -T
yr (tyrosine) and FG synthesized from (1-11) in vitro
The F acidic peptide has the following sequence.

Phen (phenylalanine) -Asn
(Asparagine) -Leu (leucine) -Pro (proline) -Leu (leucine) -Gly (glycine) -A
sn (asparagine) -Tyr (tyrosine) -Lys
(Lysine) -Lys (Lysine) -Pro (Proline) Fibroblasts: 100 ng / ml-100 mg / ml 1. Mesothelial cell growth cofactor (ECGS) was extracted from the hypothalamus by the freeze-drying method. This growth cofactor, in other words, human corneal sarcoma cells, human umbilical sarcoma cells, and mouse Balb / C fibroblasts, promotes mitosis widely and markedly in vitro. It is a factor that The skin cell growth cofactor is 200
It can be used in the concentration range of μg / ml-500 mg / ml.

3. Urogastron or epidermal growth factor (EGF) is a single-chain polypeptide composed of 53 amino acids and three disulfide bonds, and is a mouse mandibular gland secretion (mEGF), human urine (hE).
GF). It is shown that this growth factor is a factor that significantly promotes mitosis in vitro over a wide range including neuroectodermal and mesoderm.

The base sequence of artificially isolated mouse EGF is as follows.

Asn (asparagine) -Ser (serine) -Tyr (tyrosine) -Pro (proline) -Gl
y (glycine) -Cys (cysteine) -Pro (proline) -Ser (serine) -Ser (serine) -Tyr
(Tyrosine) -Asp (aspartic acid) -Gly (glycine) -Tyr (tyrosine) -Cys (cysteine)
-Leu (leucine) -Asn (asparagine) -Gl
y (glycine) -Gly (glycine) -Val (valine) -Cys (cysteine) -Met (methionine)-
His (histidine) -Ile (isoleucine) -Gl
u (glutamic acid) -Ser (serine) -Leu (leucine) -Asp (aspartic acid) -Ser (serine)
-Tyr (tyrosine) -Thr (threonine) -Cys
(Cysteine) -Asn (Asparagine) -Cys (Cysteine) -Val (Valine) Ile (Isoleucine)
-Gly (glycine) -Tyr (tyrosine) -Ser
(Serine) -Gly (glycine) -Asp (aspartic acid) -Arg (arginine) -Cys (cysteine)
-Gln (glutamine) -Thr (threonine) -Ar
g (arginine) -Asp (aspartic acid) -Leu
(Leucine) -Arg (Arginine) -Trp (Tryptophan) -Trp (Tryptophan) -Glu (Glutamic acid) -Leu (Leucine) -Arg (Arginine) and artificially isolated EGF [Cys (Acm) 2.
0'31] (20-31) Cys (cysteine)-(Acm) -Met (methionine) -His (histidine) -Ile (isoleucine)
-Glu (glutamic acid) -Ser (serine) -Leu
(Leucine) -Asp (aspartic acid) -Ser (serine) -Tyr (tyrosine) -Thr (threonine)-
Cys (cysteine) and human EGF artificially isolated receptor peptide: Asp (aspartic acid) -Val (valine) -Val (valine) -Asp
(Aspartic acid) -la (alanine) -Asp (aspartic acid) -Glu (glutamic acid) -Tyr (tyrosine) -Leu (leucine) -Ile (isoleucine)
-Pro (proline) -Gln (glutamine) Epidermal growth factor: 0.1 μg / ml-100 mg / ml 4. Bovine pituitary extract (BPF) is a water-soluble extract of the secretory glands of the pituitary gland of cattle or humans. This growth-promoting factor is shown to be a prominent mitogen in vitro on epithelial cells, in other words, human corneal epithelial cells and human epithelial keratinocytes (keratinocytes). .

Bovine pituitary extract: 100 ng / ml-50
0 mg / ml 5. Insulin is a polypeptide hormone having a mechanism of action that regulates glucose metabolism of cells and synthesizes cells, proteins, RNA and neutral fat.

Insulin: 0.1 μg / ml-10 mg
/ Ml 6. Transferrin: 0.1 μg / ml-10 mg /
ml 7. Sodium selenite: 0.1 ng / ml-100
μg / ml e. Extracellular interstitial protein 1. Type IV collagen: Main collagen of basement membrane The concentration range of type IV collagen is about 5 mg / ml-10.
g / ml.

2. Enatin concentration range is about 1 μg / m
1-100 mg / ml.

F. Buffering agent: 1. HEPES buffer (N'-2-hydroxyet
hylpiperazine-N'-Ethenesu
lfonic Acid) HEPES buffer: 10 mM-250 mM 2. Bicarbonate solution (bicarbonate solution): 0.01 mM to 250 mM g. Energy source: 1. Pyruvate: 5 mM-100 mM 2. Dextrose (D-glucose): 0.5 mM-
100 mM h. Amino acid: 0.01 mM-50 mM 1. Cystine 2. Inosine 3. Adenine 4. Adenosine i. Cell membrane fat precursors: 1. Ethanolamine: 0.01 mM-20 mM 2. Phosphoethanolamine: 0.01 mM-20 mM 3. Sialic acid: 0.001 mM-10 mM A balanced salt solution to which an additive not containing cystine was added
Used, the cat lens containing glutathione was washed for 2 hours
2-mercaptoethanol, which exhibits an effective effect in vitro, exhibits the same effect in vivo as shown by the following results.

Additive (chondroitin sulfate 4.0 mg
/ Ml; 2-mercaptoethanol 0.5 mM; dextrose (D-glucose) 0.92 mg / ml;
Pirpinic acid 1.0 mM; Bicarbonate solution (bicarbonate solution); 25 mM) (without cystine) was used to perform a 2-hour perfusion test on the anterior chamber of the cat's eye. , Perfusion of glutathione in the lens 24
A comparative study was conducted with a comparative example in which no cleaning was performed for a time. ──────────────────────────────────── Washing with additives Comparative example Glutathione total amount 1530.27 ± 26.03 μg / Lens 1435.39 ± 46.39 μg / lens 6.20% difference ───────────────────────────────────── There was a difference between the washed lens and the lens used as a comparative example due to an increase in the total amount of glutathione of 6.2%. This increase in glutathione content is due to de novo synthesis (at the amino acid level).
is)). A balanced salt solution containing an additive containing 3 mM cystine
Used, the cat lens containing glutathione was washed for 2 hours
If the effect is exhibited in vivo by the additive of the detergent containing 2-mercaptoethanol, the effect can be enhanced by adding 3 mM of cystine as shown by the following results.

A 2-hour perfusion test was performed on the anterior chamber of the cat's eye using a balanced salt solution supplemented with an additive having 3 mM cystine, and the glutathione in the lens was not washed for 24 hours after perfusion. We compared it with an example. ──────────────────────────────────── Washing containing 3 mM cystine Comparative example Total amount of BSS glutathione with added liquid 1495.73 ± 35.75 μg / lens 1378.25 ± 31.25 μg / lens 7.85% difference ──────────────────────────────── There was a difference between the washed lens and the lens used as a comparative example due to an increase in the total amount of glutathione of 7.85%. This increase in glutathione content is attributed to de novo synthesis (at the amino acid level).
sis).

It was revealed that the effect of 2-mercaptoethanol was enhanced in the balanced salt solution to which the additive having 3 mM cystine was added, by further increasing the glutathione concentration in the lens in vivo. 2-Mel to human lens and rabbit lens in a test tube
Effects of Captoethanol Results from studies in human corneal endothelial cells confirmed the limited ability to synthesize cystine via the methionine metabolic pathway. Furthermore, the endothelial cells of the cornea have a reduced ability to take up cystine, and intracellular cystine and glutathione are present in a considerably reduced amount from the standard culture medium (standard culture medium) containing cystine. Therefore, this test quantified the content of glutathione in the lens of humans and rabbits.

Reduced glutathione contained in the human lens is associated with the development of several types of cataracts. Glutathione is composed of three types of amino acids, cystine, glutamic acid, and glycine.
Cystine is classified as a restricted amino acid for the synthesis of proteins such as glutathione.

The decrease in intracellular cystine accumulation due to the addition of 2-mercaptoethanol causes a decrease in cell glutathione content. This is because the amount of cystine accumulated in cells is extremely small compared with glutamic acid and glycine.

In a test in which rabbit lenses were incubated with 2-mercaptoethanol for 3 days in vitro, glutathione content in the lenses was compared with comparative examples of lenses incubated without mercaptoethanol. ──────────────────────────────────── 2-mercaptoethanol-containing medium (culture medium) Comparative example Total glutathione 657.61 ± 24.70 μg / lens 615.51 ± 24.70 μg / lens Oxidation rate (percent) 3.94 8.54 Difference in total glutathione 6.4% ─────────────────── ───────────────── From these tests, it was found that the difference in the total amount of glutathione was very small. The rabbit lens is capable of producing sufficient cystine necessary to maintain intracellular glutathione levels while the lens is incubated in vitro. 2-mercaptoethanol can maintain the reduced state of glutathione remarkably more than the comparative example.

In a test in which a human lens was incubated with 2-mercaptoethanol in a test tube for 3 days, the glutathione-containing lens was compared and compared with a comparative example of a lens incubated without mercaptoethanol. ──────────────────────────────────── 2-mercaptoethanol-containing medium (culture medium) Comparative example Total glutathione 230.91 ± 15.49 μg / lens 176.16 ± 2.26 μg / lens Percent oxidation 13.02 17.87 Difference in total glutathione by 23.70% ─────────────────────── In the medium (culture medium) containing 2-mercaptoethanol, the total amount of glutathione mainly containing reduced glutathione increased by 23.7%.

Further, glucose is a balanced salt solution in which glucose and 1 to 5 kinds of cornea and retinal enhancing factors are added to define the normality. These (corneal and retinal enhancing factors described above) are chondroitin sulfate, 2-mercaptoethanol, pyruvic acid, cystine and bicarbonate solution (bicarbonate solution).

For example, in the implantation of a lens inside the eyeball,
In routine ophthalmic surgery, which is quite commonly performed, the exposure time to the solution is usually 3 minutes to 8 minutes.
A short and limited period of minutes. However, in special cases, the anterior chamber is kept as it is until the solution is fully filled and the aqueous solution of the eye is regenerated. The condition can last for 24 hours. During this time, cells in the anterior chamber of the eye cease to consume the required nutrients, which are usually supplied from aqueous solutions. Even in the cells of the anterior segment, the most important corneal endothelium, nutrients are supplied from those segments and most of the metabolic uptake is from the anterior surface.

The corneal endothelium maintains the transparency of the cornea by actively transporting interstitial fluid and salts from the inside of the anterior chamber of the eye to the outside of connective tissue. The sodium / potassium ATPase pumps of these endothelial cells require ATP (adenosine triphosphate) and reduce pumps (reduc).
ed pump) is provided to maintain the function of this pump. When the pump behaves as the endothelium is reduced, the cornea takes up fluid and forms a suspension that loses clarity.

One of the major disadvantages of the reducing agent glutathione and BSS Plus with bicarbonate buffering system is that the once prepared solution lacks (liquid) stability. The glutathione component is added alone and the solution is only stable for 24 hours. BSS
Plus's bicarbonate buffering capacity is already significantly reduced over solutions once exposed to the atmosphere.

The additive according to the invention supplies 2-mercaptoethanol, a reducing agent that serves both reduction and oxidation in eliminating the instability of glutathione contained in the wash solution. The effect of this reducing agent can be exerted and utilized by the epidermal cells of the human cornea.

The components of the five additives are added to the solution in order to improve the effect of repairing and protecting the anterior part of the spherical surface of the cornea during and after the trauma caused by the operation.

(1) Chondroitin sulfate, which is a glycosaminoglycan that is negatively charged with a high charge, moved from the damaged site or the ruptured site due to the movement of fluid aqueous humor to the surface of corneal epidermal cells. Added to supplement glycosaminoglycans. Glycosaminoglycans are required for membrane (potential) stabilization and maintenance of the protein receptor trilayer. These protein receptors are required for the progression of cellular metabolic processes. Chondroitin sulphate coats a protective membrane on the front of the circular part of the cell.

Additives (2) Pyruvate is supplied after the surgical trauma to additional biochemical synthetic processes required by the anterior part of the circular part of these cells. There are 20 standard amino acids that form 20 standard proteins with completely different carbon skeletons. At the same time, they have 20
Have different catabolic pathways. The 10 amino acid carbon skeleton is eventually destroyed upon entry into the citric acid cycle to produce acetyl-CoA. 10
Five of them are altered by the pyruvate pathway. The five amino acids that enter the pyruvate pathway are alanine, cystine, glycine, serine, and threonine.

(3) 2-Mercaptoethanol, an antioxidant is used to instruct the addition of the detergent, and 10 μm is used.
The effect is exhibited in the concentration range of M to 1000 μM, and its action is similar to that of macrophages of corneal endothelial cells or cells of the supply cell layer. Said substances trigger a low volume of cystine synthesis in human endothelial cells by the methionine pathway. Methylnin supplies the molecule of sulfur and serine supplies the carbon chains used for the biosynthesis of cystine. In the final reaction, the disulfide group (SH group) derived from methionine and serine is replaced with the hydroxyl group (-OH group) of serine.

In addition, corneal endothelial cells lack sufficient cystine absorption capacity to reduce intracellular cystine and glutathione to a certain threshold in normal medium containing cystine. Thus, in the presence of 2-mercaptoethanol, corneal endothelial cells are constantly available.

(4) Cystine, cell cystine, and glutathione in the wash solution are maintained by perfusion inside the eye. This effect is the most prominent function of 2-mercaptoethanol, which stimulates growing cells, and can be explained as reduction of cystine to each other in the wash solution. It is a disulphide bond between 2-mercaptoethanol and cystine consisting of the same structural formula of cystine and 2-mercaptoethanol in the wash solution,
It plays an important role under the action of 2-mercaptoethanol.

2-Mercaptoethanol repeatedly acts as a carrier for cystine. 2-mercaptopurine is removed by the perfusate without accumulating intracellularly and reacts with cystine. Thus, 2-mercaptoethanol causes cells to continuously condense due to the disulfid bond structure with cystine, and returns the perfusion to the original structural formula. With the cyclic assistance of 2-mercaptoethanol on this cystine, cells can constantly utilize cystine.

(5) The bicarbonate buffer mechanism is the intracellular p
The intracellular fluid is important for retaining H, fluidity, or the membrane potential developed in mammalian cells such as corneal endothelial cells.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification code Internal reference number FI Technical indication location A61K 31:70) (A61K 31/725 31:19) 8413-4C (72) Inventor Richard El. Lindstrom Lakeview Avenue, Excelsior, 55331, Minnesota, USA 20050 (72) Inventor Debra El. Skelnick, Minnesota, USA 55008, Cambridge, Route 5, Box 344

Claims (25)

[Claims] 1. A balanced salt solution or a solution similar thereto, comprising: a. Glycosaminoglycans, b. An antioxidant, c. At least one or more energy sources, d. Cystine, e. A buffer solution, which is an additive for a cleaning solution and a surgical solution, which contains at least one component selected from the group consisting of a buffer, and the concentration of the component in the additive for the cleaning solution and the surgical solution is , For a concentrate of 500 parts by volume of balanced salt buffer solution 1-
An additive for irrigation solution and surgical solution, which contains 100 parts by volume. 2. The cleaning solution and the additive for surgical solution according to claim 1, wherein the glycosaminoglycan is composed of chondroitin sulfate, the antioxidant is composed of 2-mercaptoethanol, and the energy source is dextrose. An additive for irrigation solutions and surgical solutions, which comprises at least one of pyruvic acid, and the buffering agent is a bicarbonate solution. 3. The irrigation solution and surgical solution additive according to claim 2, wherein the components have the following concentrations. Chondroitin sulfate 0.001-550mg / ml 2-mercaptoethanol 0.1-10mM Energy source 0.5-100mM cystine 0.01-50mM Sodium bicarbonate 0.01-100mM 4. The additive for irrigation solution and surgical solution according to claim 1, wherein the balanced salt solution contains the following components. Chondroitin sulfate 44 mg / ml 2-mercaptoethanol 5.5 mM dextrose 55 mM pyruvate 11 mM cystine 33 mM sodium bicarbonate 275 mM 5. An additive for a lavage solution and a surgical solution, which comprises the following composition in an aqueous solution. Chondroitin sulfate 44 mg / ml 2-mercaptoethanol 5.5 mM dextrose 55 mM pyruvate 11 mM cystine 33 mM sodium bicarbonate 275 mM 6. An irrigation solution and surgical solution additive, which comprises a freeze-dried component having the following composition in an aqueous solution. Chondroitin sulfate 44 mg / ml 2-mercaptoethanol 5.5 mM dextrose 55 mM pyruvate 11 mM cystine 33 mM sodium bicarbonate 275 mM 7. The concentration of the components in the wash solution as well as the additive of the surgical solution is from 1 to 100 parts by volume of concentrate to 500 parts by volume of balanced salt solution. 8. A balanced salt solution having the following composition: a. Glycosaminoglycans, b. At least one or more energy sources, c. Buffer, concentration of component of concentrate is 1-100 parts by volume of concentrate to 500 parts by volume of balanced salt solution, additive of irrigation solution and surgical solution. 9. An aqueous solution having the following composition: a. Glycosaminoglycans, b. At least one or more energy sources, c. Buffer, concentration of component of concentrate is 1-100 parts by volume of concentrate to 500 parts by volume of balanced salt solution, additive of irrigation solution and surgical solution. 10. An aqueous solution containing a lyophilized component having the following composition, a. Glycosaminoglycans, b. At least one or more energy sources, c. Buffer, lyophilized component concentration is 1-100 parts by volume of concentrate to 500 parts by volume of balanced salt solution, additive of irrigation solution and surgical solution. 11. The additive for irrigation solution and surgical solution according to claim 11, wherein the glycosaminoglycan comprises chondroitin sulfate, the antioxidant comprises 2-mercaptoethanol, and the energy source is dextrose. An additive for irrigation solutions and surgical solutions, which comprises at least one of pyruvic acid, and the buffering agent is a bicarbonate solution. 12. The additive for irrigation solution and surgical solution according to claim 11, wherein the components of the additive for irrigation solution and surgical solution have the following concentrations. Chondroitin sulfate 0.001-550 mg / ml Energy source 0.5-100 mM Sodium hydrogen carbonate 0.01-100 mM 13. An additive for irrigation solution and surgical solution, which comprises the following components in a balanced salt solution. Chondroitin sulfate 44 mg / ml Dextrose 55 mM Sodium pyruvate 11 mM Sodium bicarbonate 275 mM 14. An additive for a lavage solution and a surgical solution, which comprises the following components in an aqueous solution. Chondroitin sulfate 44 mg / ml Dextrose 55 mM Sodium pyruvate 11 mM Sodium bicarbonate 275 mM 15. An additive for a lavage solution and a surgical solution, which comprises a freeze-dried component having the following composition in an aqueous solution. Chondroitin sulfate 44 mg / ml Dextrose 55 mM Sodium pyruvate 11 mM Sodium bicarbonate 275 mM 16. An additive for an ophthalmic solution, which has the following composition. a. Glucosaminoglucan b. Antioxidant c. Energy source d. Amino acids e. Buffer 17. An additive for an ophthalmic solution, which comprises the following components. a. Chondroitin sulfate b. 2-mercaptoethanol c. Dextrose or alkaline pyruvate (a
lkali pyruvate) d. Cystine e. sodium hydrogen carbonate 18. An equilibration buffered salt solution or a solution similar thereto, comprising: a. Glycosaminoglycans, b. An additive for irrigation solution and surgical solution, which contains a solution of at least one component selected from the group consisting of at least one energy source. 19. An additive for a lavage solution and a surgical solution, which comprises the following components in an aqueous solution. a. Glycosaminoglycan b. At least one type of energy source 20. An additive for a lavage solution and a surgical solution, which comprises the following lyophilized components in an aqueous solution. a. Glycosaminoglycan b. At least one type of energy source 21. The additive for irrigation solution and surgical solution according to claim 20, wherein the glycosaminoglycan is chondroitin sulfate. 22. The irrigation solution and surgical solution additive according to claim 20, wherein the energy source is dextrose. 23. A balanced salt solution or a solution similar thereto, comprising: a. Clicosaminoglycans, b. At least one or more energy sources, c. An additive for a washing solution and a surgical solution, which contains at least one or more lyophilized components selected from the group consisting of at least one or more cell-forming factors or growth cofactors. 24. The additive for irrigation solution and surgical solution according to claim 23, wherein the glycosaminoglycan comprises chondroitin sulfate, the energy source comprises dextrose, and the cell growth factor comprises EGF and insulin. An additive for irrigation solutions as well as surgical solutions. 25. The irrigation solution and surgical solution additive according to claim 23, wherein the glycosaminoglycan comprises chondroitin sulfate, the energy source comprises dextrose, and the cell growth factor comprises EGF and insulin. , An additive for irrigation and surgical solutions, characterized in that it consists of a solution, transferrin, and a selenite solution.