JPH0664035B2 - Preparative method in gas chromatograph - Google Patents
Preparative method in gas chromatographInfo
- Publication number
- JPH0664035B2 JPH0664035B2 JP21129285A JP21129285A JPH0664035B2 JP H0664035 B2 JPH0664035 B2 JP H0664035B2 JP 21129285 A JP21129285 A JP 21129285A JP 21129285 A JP21129285 A JP 21129285A JP H0664035 B2 JPH0664035 B2 JP H0664035B2
- Authority
- JP
- Japan
- Prior art keywords
- syringe
- gas chromatograph
- needle
- collected
- components
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明はガスクロマトグラフにおける分離成分の分取方
法に関する。TECHNICAL FIELD The present invention relates to a method for separating separated components in a gas chromatograph.
ガスクロマトグラフにより分離成分を分取する際には、
分離された成分をガスクロマトグラフ出口部に設けられ
た深冷トラツプで捕集していた。When collecting separated components by gas chromatography,
The separated components were collected by a deep-chill trap provided at the gas chromatograph outlet.
〔発明が解決しようとする問題点〕 しかしながら、このような方法では、分取した成分をさ
らに他の方法で分析する場合には、トラツプに捕集され
た成分を溶媒で溶解してからサンプリングするため、折
角捕集した成分を100％利用することが困難であり、特
に目的成分が微量の場合には不都合である。本発明はガ
スクロマトグラフにおける分離成分の分取に際し、特に
微量成分を有効に利用しうるように分取する方法を提供
することを目的とするものである。[Problems to be Solved by the Invention] However, in such a method, when the separated components are analyzed by another method, the components collected in the trap are dissolved in a solvent before sampling. Therefore, it is difficult to use 100% of the collected components, which is inconvenient especially when the target component is in a small amount. An object of the present invention is to provide a method for separating a separated component in a gas chromatograph so that a trace amount of the component can be effectively used.
上述の本発明の目的は、冷却剤が収容されている容器中
にシリンジの針を収納し、該シリンジの針のシリンジ接
続部をガスクロマトグラフの分離カラムに接続されてい
る配管の出口部に接続して所望の成分を分取することに
より達成される。以下、本発明を詳細に説明する。The above-described object of the present invention is to store a needle of a syringe in a container in which a coolant is stored, and connect a syringe connecting portion of the needle of the syringe to an outlet portion of a pipe connected to a separation column of a gas chromatograph. It is achieved by separating the desired components. Hereinafter, the present invention will be described in detail.
第１図はガスクロマトグラフの一例の模式図であり、１
は恒温槽、２はキヤリアガス導入部３はキヤリアガス導
管、４は試料注入部、５は分離カラム、６は対称カラ
ム、７は検出器である。本発明においては所望の成分を
冷却したシリンジの針に捕集する。即ち、分離カラム５
で分離された成分を検出器７で検知し、目的成分が流出
してきたことが確認されたら、冷却剤を収容した容器中
に収納されたシリンジの針のシリンジ接続部をガスクロ
マトグラフの分離カラムに接続されている配管の出口部
に接続して目的成分を捕集する。第２図は本発明方法を
実施するのに好適な分取装置の一例の模式図であり、10
はシリンジの針、10−１はそのシリンジ接続部、11は冷
却剤、12はシリンジの針及び冷却剤を収容する容器であ
る。冷却剤としては目的成分に応じて、液体窒素、ドラ
イアイス、氷等が用いられる。なお、液体窒素及び氷を
用いる場合はシリンジの針の内部に液体が侵入しないよ
うにする必要がある。このような分取装置８を、容器12
に収納されているシリンジの針10のシリンジ接続部10−
１が分離カラム５に接続されている配管９の出口部と接
続するようにして（第１図参照）目的成分を分取する。
該針のシリンジ接続部は該配管の出口部と脱着自在とな
るように相互に構成されており、目的成分以外の成分が
流出しているときは、分取装置を配管の出口部から取外
すことができる。目的成分の捕集が終了したら、分取装
置を配管の出口部から取外し、シリンジ接続部にゴムキ
ヤツプ等をかぶせて空気中の水分等の混入を防止する。
この操作を繰返すことにより、多数回の分取が可能であ
る。FIG. 1 is a schematic diagram of an example of a gas chromatograph.
Is a thermostatic chamber, 2 is a carrier gas introduction part 3, 3 is a carrier gas conduit, 4 is a sample injection part, 5 is a separation column, 6 is a symmetric column, and 7 is a detector. In the present invention, the desired component is collected by a cooled syringe needle. That is, the separation column 5
When it is confirmed that the target component has flowed out by detecting the component separated by the detector 7, the syringe connection part of the needle of the syringe housed in the container containing the coolant is connected to the separation column of the gas chromatograph. The target component is collected by connecting to the outlet of the connected pipe. FIG. 2 is a schematic view of an example of a sorting device suitable for carrying out the method of the present invention.
Is a needle of a syringe, 10-1 is a syringe connection portion thereof, 11 is a coolant, and 12 is a container for containing the needle of the syringe and the coolant. As the cooling agent, liquid nitrogen, dry ice, ice or the like is used depending on the target component. When using liquid nitrogen and ice, it is necessary to prevent the liquid from entering the inside of the needle of the syringe. Such a sorting device 8 is provided with a container 12
Syringe connection part 10 of the needle 10 of the syringe stored in
1 is connected to the outlet of the pipe 9 connected to the separation column 5 (see FIG. 1), and the target component is fractionated.
The syringe connection part of the needle is configured so as to be detachable from the outlet part of the pipe, and when a component other than the target component is flowing out, remove the fractionation device from the outlet part of the pipe. You can When the collection of the target component is completed, the preparative device is removed from the outlet of the pipe, and the syringe connection is covered with a rubber cap or the like to prevent the inclusion of water in the air.
By repeating this operation, a large number of fractions can be collected.
かくして捕集された成分をさらにガスクロマトグラフ質
量分析計或はガスクロマトグラフ赤外分光光度計により
分析する場合は、第３図に示すように適当な溶媒13を収
容したシリンジ14にシリンジの針を接続し、該針の中に
捕集されている成分を該溶媒で溶解しつつ、上記分析計
の試料注入部15に注入する。When the components thus collected are further analyzed by a gas chromatograph mass spectrometer or a gas chromatograph infrared spectrophotometer, the syringe needle is connected to a syringe 14 containing an appropriate solvent 13 as shown in FIG. Then, the components collected in the needle are dissolved in the solvent and injected into the sample injection part 15 of the analyzer.
本発明方法によれば、目的成分を極めて簡便に捕集する
ことができ、また捕集された成分をさらに他の方法によ
り分析する場合に分取した成分を有効に利用することが
でき、本発明方法は特に分取成分が微量の場合に有利で
ある。According to the method of the present invention, the target component can be collected very easily, and the collected component can be effectively used when the collected component is analyzed by another method. The inventive method is particularly advantageous when the amount of preparative components is small.
第１図はガスクロマトグラフの一例の模式図、第２図は
本発明方法の実施に好適な装置の一例の模式図、第３図
は本発明で得られたガスクロマトグラフの分取成分を他
の分析法に適用する際の態様の一例の模式図である。 １……恒温槽、５……分離カラム、８……分取装置、10
……シリンジの針、11……冷却剤、14……シリンジFIG. 1 is a schematic diagram of an example of a gas chromatograph, FIG. 2 is a schematic diagram of an example of an apparatus suitable for carrying out the method of the present invention, and FIG. 3 is a schematic diagram of other components of the gas chromatograph obtained by the present invention. It is a schematic diagram of an example of the aspect at the time of applying to an analytical method. 1 ... Constant temperature bath, 5 ... Separation column, 8 ... Preparative device, 10
…… Syringe needle, 11 …… Coolant, 14 …… Syringe
Claims (1)
の針を収納し、該シリンジの針のシリンジ接続部をガス
クロマトグラフの分離カラムに接続されている配管の出
口部に接続して、所望の成分を分取することを特徴とす
るガスクロマトグラフにおける分取方法。1. A needle of a syringe is housed in a container containing a coolant, and a syringe connecting portion of the needle of the syringe is connected to an outlet portion of a pipe connected to a separation column of a gas chromatograph, A method for preparative separation in a gas chromatograph, characterized by preparatively separating desired components.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21129285A JPH0664035B2 (en) | 1985-09-25 | 1985-09-25 | Preparative method in gas chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21129285A JPH0664035B2 (en) | 1985-09-25 | 1985-09-25 | Preparative method in gas chromatograph |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6270756A JPS6270756A (en) | 1987-04-01 |
| JPH0664035B2 true JPH0664035B2 (en) | 1994-08-22 |
Family
ID=16603515
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21129285A Expired - Lifetime JPH0664035B2 (en) | 1985-09-25 | 1985-09-25 | Preparative method in gas chromatograph |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0664035B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4609943B2 (en) * | 2005-12-12 | 2011-01-12 | 曽田香料株式会社 | Method and apparatus for continuous evaluation of volatile components |
| CN105784916B (en) * | 2016-03-02 | 2017-08-08 | 国家海洋局第三海洋研究所 | The cadmium post loading method determined for water quality nitrate |
-
1985
- 1985-09-25 JP JP21129285A patent/JPH0664035B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6270756A (en) | 1987-04-01 |
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