JPH065500U - Hairy root-like plant tissue culture device - Google Patents

Abstract

(57) [Abstract] [Purpose] Not only does the exfoliation and destruction of plant tissue in culture not occur, but also the carrier on which hairy root-like plant tissue is implanted can be easily collected for industrial production with good handleability. A culture device for hairy root-like plant tissue is provided. [Constitution] Cultivation tank 10, granular carrier B for implanting plant tissue in culture tank 10, and sprinkling nozzle 31 for spraying culture solution R extracted from culture tank 10 into culture tank 10.
And a circulation means 30 for supplying the culture solution to the sprinkling nozzle, and a granular carrier outlet 12 provided at the bottom 11 of the culture tank 10.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to an apparatus for culturing hairy root-like plant tissue, which can easily collect a carrier to which hairy root-like plant tissue such as Korean carrot or horseradish is attached from a culture tank.

[0002]

[Prior art]

 BACKGROUND ART Conventionally, as an apparatus for culturing hairy root-like plant tissue, there are an air lift type apparatus and a stirring type apparatus.

[0003]

[Problems to be solved by the device]

 However, both are small-scale equipment at the laboratory level, and have the following problems as equipment for large-scale industrial production.

That is, in the former air lift type apparatus, the inner cylinder (draft tube) for air lift is made of polyurethane or wire mesh, and the hairy root-like plant tissue is implanted and propagated using this as a carrier.

Therefore, most cell tissues grow by being entangled with the polyurethane or the wire mesh, and therefore, in order to recover the plant tissue after the completion of the culture, the lid on the upper part of the culture tank must be removed to take out the inner cylinder. Absent. This is not a problem with a culture tank of about 2 to 10 liters, but in the case of an industrial production scale culture tank, this is a work that requires a great deal of work if not impossible.

[0006] In general, useful products are present in plant tissues, and unless plant tissues can be easily recovered, they are not industrial devices. Further, in the air circulation type device of the internal circulation system, the packing material is disturbed and the shearing force causes the detachment of the implantation tissue.

On the other hand, in the latter stirring type device, the culture solution is circulated by a turbine type stirring device instead of an air lift, and a cylindrical wire net is used as a carrier inside the device.

Therefore, the recovery of the plant tissue has the same problem as the above-mentioned air lift type device. In addition, the agitation type device causes disruption due to shearing of cells even when the amount of cells is small, and the plant tissue for hairy roots is entangled in the rotary blades with an increase in the amount of cells. Occurs and it becomes difficult to continue the culture.

The present invention has been made to overcome such conventional problems, and its purpose is not only to prevent detachment and destruction of plant tissues in culture, but also to prevent hairy root-like plant tissues from attaching. An object of the present invention is to provide a device for culturing hairy root-like plant tissue, which is suitable for large-scale production and has good handleability, in which the bed carrier can be easily recovered.

[0010]

[Means for Solving the Problems]

 The present invention is directed to a culture tank, a granular carrier for implanting plant tissue filled in the culture tank, a spray nozzle for spraying the culture solution extracted from the culture tank into the culture tank, and a culture solution for the spray nozzle. The present invention provides a hairy root-like plant tissue culturing apparatus, characterized by comprising a circulation means for supplying water and a granular carrier outlet provided at the bottom of the culture tank.

[0011]

[Action]

 In this way, the granular carrier for implanting plant tissue is filled in the culture tank, the culture solution is extracted from the culture tank, and sprayed into the culture tank by the circulation means and the water spray nozzle. Then, when a predetermined amount of the plant tissue is implanted on the granular carrier, the granular carrier outlet provided at the bottom of the culture tank is opened and taken out of the tank. According to such a culture device, not only does the peeling or destruction of the plant tissue in culture in the culture tank not occur, but also the granular carrier on which the hairy root-like plant tissue is implanted can be easily recovered.

[0012]

【Example】

 An embodiment of the present invention will be described below with reference to the drawings. In this example, an external circulation system using a pump is used as an example of the culture solution circulation means.

In FIG. 1, 10 is a culture tank. This culture tank 10 has a one-sided cone shape with one side of the bottom portion 11 having an inclination, and the spherical carrier B can be taken out smoothly without forming a bridge. Further, an end plate-shaped hatch 13 is openably and closably attached to the carrier outlet 12 provided at the lower end of the bottom 11. Further, inside the hatch 13, a wire net 14 for supporting the carrier B is attached. In addition, a wedge wire screen 15 is arranged in parallel on the inclined portion of the bottom portion 11 while maintaining a predetermined interval.

Further, a carrier input port 16 is attached to the upper side surface of the culture tank 10, and the spherical carrier B is filled in the culture tank 10 from the input port 16. The carrier B is, for example, a sponge-shaped and ball-shaped spherical ball made of a polymeric material such as polyurethane. In addition, if it is porous and granular, it is made of wire mesh. The ruching, etc. can also be preferably used.

Further, the end plate 17 at the top of the culture tank is equipped with an air introduction pipe 20 provided with a culture liquid supply pipe 18 and an air filter 19, and the culture liquid supply pipe 18 is used to culture the culture medium in the culture tank 10. The liquid R is filled and clean air A is supplied from the air introduction pipe 20.

On the other hand, the culture solution R in the culture tank 10 is circulated by an external circulation system. The external circulation path 30 is provided between the culture tank 10, the spray nozzle 31, which is arranged directly below the end plate 17 of the culture tank, the pump 32, the suction port 33 of the pump, the hatch 13 and the culture tank bottom 11. It is composed of conduits 35 and 36, and a conduit 37 arranged between the pump discharge port 34 and the spray nozzle 31, and when the pump 32 is operated, the culture solution R in the culture tank 10 becomes 10 → conduit 35 and 36 → pump 32 → conduit 37 → spray nozzle 31 → circulation in the culture tank 10. However, as the culture solution R is sprayed in the form of fine particles from the spray nozzle 31, oxygen in the air in the culture tank 10 is dissolved in the culture solution R to become oxygen-rich. As the pump 32, a diaphragm or tube pump that is easily steam-sterilized is suitable.

Furthermore, a jacket 38 for heating or cooling is attached to a conduit 37 between the pump 32 and the spray nozzle 31, so that the circulating culture solution R is adjusted to a predetermined temperature. . Further, a heat insulating material 39 is attached to the outside of the jacket 38 and the culture tank 10.

Furthermore, a temperature indicator controller 21, a pH indicator controller 22 and a D 2 O sensor (dissolved oxygen concentration meter) 23 are attached to the culture tank 10, and the values measured by these instruments are stored in a microcomputer. A control device (not shown) controls the system to ensure optimal operation.

Next, the operation of the culture device will be described. As shown in FIG. 1, a culture medium 10 is filled with a granular carrier B and a culture solution R containing hairy root-like plant tissue, and then air A is introduced into the culture vessel 10 through an air introduction pipe 20. At the same time, when the pump 32 is operated while supplying warm water to the jacket 38 and maintaining the culture solution R at a predetermined temperature, the culture solution R in the culture tank 10 is circulated through the external circulation path 30.

Therefore, the disturbance of the carrier B caused by the aeration in the liquid such as the air lift type device does not occur. Moreover, oxygen in the air in the culture tank 10 is dissolved in the culture solution R sprayed from the spray nozzle 31 to become oxygen-rich, and the hairy root-like plant tissue is cultured under a good environment. Be seen.

When the hairy root-like plant tissue grows to a predetermined size, the supply of air to the culture tank 10, the supply of warm water to the jacket 38, and the operation of the pump 32 are stopped. Next, after the culture solution is stored and transferred, the hatch 13 at the bottom of the culture tank is opened, and the carrier B on which the hairy root-like plant tissue grown in the culture tank 10 has been implanted is removed from the carrier outlet 12. It is discharged smoothly. Then, these carriers B are processed in an unillustrated downstream extraction device or the like to recover useful components.

Although the embodiment of the present invention has been described above, the present invention is not limited to this embodiment and may be modified or modified within the scope of the present invention. Of.

That is, in the above description, the case where the culture solution in the culture tank is externally circulated by the pump has been described, but an air lift in which the air inlet pipe 20a is connected to the discharge port 34 of the pump 32 is used. Is also good. In this case, there is an advantage that the solubility of oxygen in the culture solution can be increased.

[0024]

[Effect of device]

 As described above, the present invention provides a culture tank, a granular carrier for implanting plant tissue in the culture tank, a water spray nozzle for spraying the culture solution extracted from the culture tank into the culture tank, Since the circulation means for supplying the culture solution to the watering nozzle and the granular carrier outlet provided at the bottom of the culture tank are provided, peeling or destruction of hairy root-like plant tissue during culture in the culture tank does not occur. Not only that, the granular carrier on which the hairy root-like plant tissue is implanted can be easily recovered, which is very useful in industrial production.

[Brief description of drawings]

FIG. 1 is a schematic view of an apparatus for culturing hairy root-like plant tissue according to the present invention.

[Explanation of symbols]

 10 Culture Tank 11 Bottom of Culture Tank 12 Carrier Outlet 30 Circulating Means 31 Sprinkling Nozzle B Granular Carrier R Culture Solution

Claims (1)

[Scope of utility model registration request] 1. A culture tank, a granular carrier for implanting plant tissue filled in the culture tank, a spray nozzle for spraying the culture solution extracted from the culture tank into the culture tank, and a culture solution for the spray nozzle. An apparatus for cultivating hairy root-like plant tissue, comprising: