JPH0653053B2 - Low molecular fraction in oyster meat - Google Patents
Low molecular fraction in oyster meatInfo
- Publication number
- JPH0653053B2 JPH0653053B2 JP61099247A JP9924786A JPH0653053B2 JP H0653053 B2 JPH0653053 B2 JP H0653053B2 JP 61099247 A JP61099247 A JP 61099247A JP 9924786 A JP9924786 A JP 9924786A JP H0653053 B2 JPH0653053 B2 JP H0653053B2
- Authority
- JP
- Japan
- Prior art keywords
- molecular weight
- oyster meat
- low molecular
- less
- fraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Description
【発明の詳細な説明】 〔技術分野〕 本発明は、カキ肉中の低分子画分に関するものである。TECHNICAL FIELD The present invention relates to a low molecular weight fraction in oyster meat.
カキは二枚貝綱イタボガキ科に属するもので、その成分
として、グリコーゲン、ビタミン類、ミネラル、アミノ
酸、低分子ペプタイド、アデノシンモノホスフェート等
の核酸関連物質等を含むものであり、極めて栄養価に富
み、且つ独特の旨味を有するものである。Oysters belong to the family Bivalvia and other species, and as components thereof, include glycogen, vitamins, minerals, amino acids, low-molecular-weight peptides, nucleic acid-related substances such as adenosine monophosphate, etc., and are extremely nutritious, and It has a unique taste.
このカキの栄養分を有効に利用したものとして、カキ肉
エキスが知られている。Oyster meat extract is known as an effective use of the nutrients of this oyster.
ところで、現在実用化ないし提案されているカキ肉エキ
スの多くはカキ肉を水（特に、温湯）抽出し、固形分を
除去した後の上清を濃縮、乾燥したものである。By the way, most of the oyster meat extracts which have been put into practical use or proposed at present are those obtained by extracting oyster meat with water (especially hot water), removing the solid content, and concentrating and drying the supernatant.
当該公知エキス中には亜鉛等のミネラル類、アミノ酸、
タウリン、ビタミン類、ペプタイド、核酸関連物質、ム
コ多糖類、タンパク質等が含有されており、これでも十
分なる栄養食品であるが、さらにより栄養価が高く、ま
た栄養補助食品として有用なカキ肉エキスの出現が待望
される。The known extracts include minerals such as zinc, amino acids,
Oyster meat extract containing taurine, vitamins, peptides, nucleic acid-related substances, mucopolysaccharides, proteins, etc., which is a sufficient nutritional product, but has a higher nutritional value and is useful as a dietary supplement. Is expected.
本発明の目的は、より栄養価が高く、栄養補助食品とし
て有用なカキ肉エキスを提供することである。An object of the present invention is to provide an oyster meat extract having a higher nutritional value and useful as a dietary supplement.
かかる目的を達成するために本発明者らは、種々研究を
重ねてきたところ、カキ肉成分中の分子量5000以下の低
分子画分中には、特に多量のアミノ酸、タウリン、アデ
ノシン５′モノホスフェートなどの核酸関連物質および
脳中のリチウム濃度を高める作用を有する物質、血栓を
溶解する作用を有する物質、精神分裂病治療作用を有す
る物質等が含まれており、従ってかかる低分子画分を多
量に含有するカキ肉エキスはより栄養価が高く、栄養補
助食品として有用であることを見出した。In order to achieve such an object, the inventors of the present invention have conducted various studies and found that particularly large amounts of amino acids, taurine and adenosine 5'monophosphate are contained in the low molecular weight fraction having a molecular weight of 5000 or less in the oyster meat component. Nucleic acid-related substances such as and substances that have the effect of increasing the concentration of lithium in the brain, substances that have the action of dissolving blood clots, substances that have the action of treating schizophrenia, etc. It was found that the oyster meat extract contained in is more nutritious and useful as a dietary supplement.
本発明はかかる新知見に基づいて完成されたものであ
り、カキ肉成分中の分子量5000以下の低分子画分を豊富
に含有してなるカキ肉エキスである。The present invention was completed based on this new finding, and is an oyster meat extract containing abundant low molecular weight fractions having a molecular weight of 5000 or less in oyster meat components.
本発明のカキ肉エキス中に豊富に含有されている低分子
画分ないしは本発明のカキ肉エキスは、たとえば次の様
にして調製されるものである。The low molecular weight fraction abundantly contained in the oyster meat extract of the present invention or the oyster meat extract of the present invention is prepared, for example, as follows.
即ち、好ましくは、まずカキ肉を水抽出する。その際、
温水（約40〜90℃、特に約60〜85℃）にて行うことが好
ましい。抽出時間は、通常１〜４時間、好ましくは２〜
３時間である。That is, preferably, the oyster meat is first extracted with water. that time,
It is preferably carried out in warm water (about 40 to 90 ° C, especially about 60 to 85 ° C). The extraction time is usually 1 to 4 hours, preferably 2 to
3 hours.
上記水抽出物の固液を分離し、液相を、好ましくは濃縮
する。従来実用化されているカキ肉エキスは当該液相を
濃縮・乾燥したものである。本発明のカキ肉エキスにお
いては前記液相の濃縮物を、さらに高分子物質を分離・
除去する手段、換言すれば分子量5000の低分子物質のみ
を含有する画分あるいは低分子物質を豊富に含有する画
分を分離回収する手段、たとえばポリオレフィン系、合
成高分子（ポリスルフォン系等）ポリイミドを膜素材と
する限外濾過膜による方法、ゲル濾過等に付して分子量
5000以下の低分子物質のみあるいは当該低分子画分を豊
富に含む画分を回収する。その際、本発明者らは次の新
知見を得た。すなわち、カキ肉の水抽出物中には分子量
１０万以上の高分子物質と、分子量5000以下の物質の含
量が多く、分子量１０万〜5000の範囲にある物質の物質
は殆ど含まれていないことを知見した。従って、当該低
分子画分の分離・回収するにあたっては、換言すればカ
キ肉水抽出物中の高分子物質（たとえば、グリコーゲ
ン、ムコ多糖類、蛋白質等）と前記低分子物質とを分離
するにあたっては、カキ肉の水抽出物中の分子量１０万
以下の物質を分離・回収する手段、あるいは分子量5000
以上の物質を分離・除去しうる手段に付せば、十分その
目的が達成される。なお、カキ肉を分子量5000以下の物
質のみを分離・回収する手段に付すことによって分子量
5000以下の物質のみよりなる低分子画分を得ることが出
来る。The solid-liquid of the above water extract is separated and the liquid phase is preferably concentrated. The oyster meat extract that has been practically used in the past is obtained by concentrating and drying the liquid phase. In the oyster meat extract of the present invention, the liquid phase concentrate is further separated into polymer substances.
A means for removing, in other words, a means for separating and collecting a fraction containing only a low molecular weight substance having a molecular weight of 5000 or a fraction rich in a low molecular weight substance, for example, a polyolefin type, a synthetic polymer (polysulfone type, etc.) polyimide The molecular weight of the method using an ultrafiltration membrane that uses as a membrane material, gel filtration, etc.
Collect only a low molecular weight substance of 5000 or less or a fraction rich in the low molecular weight fraction. At that time, the present inventors obtained the following new findings. That is, the water extract of oyster meat contains a large amount of high molecular weight substances having a molecular weight of 100,000 or more and substances having a molecular weight of 5,000 or less, and hardly contains substances having a molecular weight of 100,000 to 5,000. I found out. Therefore, in separating and recovering the low molecular weight fraction, in other words, in separating the high molecular weight substance (for example, glycogen, mucopolysaccharide, protein, etc.) in the oyster meat water extract from the low molecular weight substance. Is a means for separating and recovering substances having a molecular weight of 100,000 or less in the water extract of oyster meat, or a molecular weight of 5,000.
The purpose can be sufficiently achieved by providing a means capable of separating and removing the above substances. In addition, the molecular weight of oyster meat can be increased by attaching it to the means for separating and collecting only substances with a molecular weight of 5000 or less.
It is possible to obtain a low molecular weight fraction consisting only of substances of 5000 or less.
さらに、本発明者らは当該低分子画分を豊富に含有する
画分を効率よく得るために研究を重ねた結果、カキ肉水
抽出液、特にその温湯抽出液を分子量１０万以下の物質
を分離回収しうる膜を使用するゲル濾過に付すことによ
って極めて効率よく当該低分子物質を含む画分が得られ
ることを見出した。従って、本発明に関して、低分子画
分ないしは本発明カキ肉エキスを得るためには、カキ肉
水抽出物を分子量１０万以下の物質を回収しうる手段に
付すことが好ましい。Furthermore, as a result of repeated studies to efficiently obtain a fraction rich in the low-molecular-weight fraction, the present inventors have found that the oyster meat water extract, particularly the hot water extract thereof, has a molecular weight of 100,000 or less. It was found that a fraction containing the low-molecular substance can be obtained very efficiently by subjecting it to gel filtration using a membrane that can be separated and collected. Therefore, regarding the present invention, in order to obtain a low molecular weight fraction or an oyster meat extract of the present invention, it is preferable to subject the oyster meat water extract to a means capable of recovering a substance having a molecular weight of 100,000 or less.
ゲル濾過による場合には、使用されるゲル濾過担体とし
て、分子量5000以下の物質を分子篩しえ、分子量１０万
以上の物質を分子篩しないもの、たとえばセファデック
スＧ１００、セファデックスＧ５０、セファデックスＧ
２５等が使用される。In the case of gel filtration, the gel filtration carrier used is capable of molecular sieving a substance having a molecular weight of 5,000 or less and not sieving a substance having a molecular weight of 100,000 or more, for example, Sephadex G100, Sephadex G50, Sephadex G.
25 etc. are used.
当該ゲル濾過における条件は次の通りである。The conditions in the gel filtration are as follows.
条件：カラムサイズ・・２０mm×1000mm 溶媒・・蒸留水 展開速度・・1.5ml/min. また、ポリオレフィン系、合成高分子（ポリスルフォン
系等）、ポリイミド系等よりなる限外濾過膜、具体的に
は、たとえＮＴＲ−３５１−１８ＬＰ（日東電気工業社
製）等を通過する亜鉛などのミネラル、タウリン、核酸
関連物質、ペプタイドを含有する低分子画分を得ること
が出来る。Conditions: Column size: 20 mm x 1000 mm Solvent: Distilled water Development speed: 1.5 ml / min. In addition, an ultrafiltration membrane made of polyolefin, synthetic polymer (polysulfone, etc.), polyimide, etc. It is possible to obtain a low-molecular-weight fraction containing minerals such as zinc, taurine, nucleic acid-related substances, and peptides that pass through NTR-351-18LP (manufactured by Nitto Denki Kogyo KK).
かくして、得られた低分子画分は、たとえばエタノール
で洗浄を３回繰り返した後乾燥し、白色の粉末結晶を得
る等の手段に付してさらに精製して分子量が5000以下で
ある物質をより多量に含有せしめてもよい。Thus, the low-molecular weight fraction obtained is subjected to a procedure such as washing with ethanol three times and then drying to obtain a white powder crystal, which is further purified to obtain a substance having a molecular weight of 5,000 or less. It may be contained in a large amount.
本発明のカキ肉エキスは、たとえば栄養補助食品、医薬
品等として有用である。The oyster meat extract of the present invention is useful, for example, as a dietary supplement, a drug, and the like.
本発明のカキ肉エキスを食品、特に栄養補助食品、医薬
品等として使用する場合、たとえば当該低分子画分をそ
のまま（好ましくは、乾燥品として）、あるいは公知の
賦形剤等とともにタブレット、カプセル、顆粒、粉末等
として調製される。また、従来既知のカキ肉エキスと混
合して使用される。When the oyster meat extract of the present invention is used as a food, in particular, as a dietary supplement, a drug, etc., for example, the low molecular weight fraction as it is (preferably as a dried product) or a tablet, capsule, or the like together with a known excipient, etc. Prepared as granules, powder, etc. Further, it is used as a mixture with a conventionally known oyster meat extract.
ところで、当該低分子画分は、一般に吸湿性であるか
ら、単独で製品化する場合には凍結乾燥に付することが
好ましい。また当該低分子画分は、従来既知のカキ肉エ
キスと混合してから乾燥することによっても、良好な乾
燥製品が得られる。By the way, since the low molecular weight fraction is generally hygroscopic, it is preferable to subject it to freeze-drying when it is produced as a single product. Also, a good dried product can be obtained by mixing the low molecular weight fraction with a conventionally known oyster meat extract and then drying.
本発明に関して、低分子画分は、タウリン、アミノ酸、
核酸関連物質、亜鉛等のミネラル類、ペプタイド類を多
量に含む他、脳中のリチウム濃度を高める作用、血栓を
溶解する作用、精神分裂病治療作用を有するものであ
る。従って、本発明のカキ肉エキスは、医薬品、栄養補
助食品等として極めて優れたものである。In the context of the present invention, low molecular weight fractions include taurine, amino acids,
In addition to containing a large amount of nucleic acid-related substances, minerals such as zinc, and peptides, it has the action of increasing the lithium concentration in the brain, the action of dissolving thrombus, and the action of treating schizophrenia. Therefore, the oyster meat extract of the present invention is extremely excellent as a medicine, dietary supplement, and the like.
実施例１ 新鮮なカキ肉１kgに対して１kgの蒸留水を添加し、８５
℃で２時間抽出を行い、固液の分離を行う。液相を、膜
としてポリスルフォン系（ＮＴＲ−３５１−Ｐ１８Ｌ
Ｐ、日東電気工業社製）を使用する工業的限外濾過法に
て分子量１０万以下の物質を含む画分（分子量5000以下
の低分子画分が豊富な画分）１０ｇを得た。その後、エ
タノールで洗浄を３回繰り返した後、凍結乾燥し、白色
の粉末結晶性のカキ肉エキスを得た。Example 1 1 kg of distilled water was added to 1 kg of fresh oyster meat, and 85
Extraction is carried out at ℃ for 2 hours to separate solid and liquid. Liquid phase as a membrane, polysulfone type (NTR-351-P18L
P, manufactured by Nitto Denki Kogyo Co., Ltd.) was used to obtain 10 g of a fraction containing a substance having a molecular weight of 100,000 or less (fraction rich in low molecular weight fraction of 5000 or less). After that, washing with ethanol was repeated 3 times and then freeze-drying to obtain a white powdery crystalline oyster meat extract.
参考例２ 実施例１で得られたカキ肉エキス１００ｇと、カキ肉を
温湯抽出（８５℃、２時間）して得たカキ肉エキス９０
０ｇを混和して粉末状カキ肉エキスを得た。Reference Example 2 100 g of oyster meat extract obtained in Example 1 and oyster meat extract 90 obtained by extracting oyster meat with hot water (85 ° C., 2 hours)
0 g was mixed to obtain powdery oyster meat extract.
Claims (3)
分を豊富に含有してなるカキ肉エキス。1. An oyster meat extract containing abundant low molecular weight fractions having a molecular weight of 5000 or less in an oyster meat component.
5000以下の物質を回収しうる手段に付して得られたもの
である特許請求の範囲第(1)項記載のカキ肉エキス。2. The low molecular weight fraction of oyster meat has a molecular weight of 100,000 or less.
The oyster meat extract according to claim (1), which is obtained by applying a means capable of recovering a substance of 5000 or less.
１０万以下〜5000以下の物質を回収しうる手段に付して
得られたものである特許請求の範囲第(1)項記載のカキ
肉エキス。3. The low molecular fraction is obtained by subjecting an aqueous extract of oyster meat to a means capable of recovering a substance having a molecular weight of 100,000 or less to 5,000 or less. The oyster meat extract according to the item.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61099247A JPH0653053B2 (en) | 1986-04-28 | 1986-04-28 | Low molecular fraction in oyster meat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61099247A JPH0653053B2 (en) | 1986-04-28 | 1986-04-28 | Low molecular fraction in oyster meat |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62257359A JPS62257359A (en) | 1987-11-09 |
| JPH0653053B2 true JPH0653053B2 (en) | 1994-07-20 |
Family
ID=14242370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61099247A Expired - Lifetime JPH0653053B2 (en) | 1986-04-28 | 1986-04-28 | Low molecular fraction in oyster meat |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0653053B2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4121583B2 (en) * | 1996-05-09 | 2008-07-23 | 日本クリニック株式会社 | Antioxidant composition and method for producing the same |
| JPH11322617A (en) * | 1998-05-07 | 1999-11-24 | Tokiwa Yakuhin Kogyo Kk | Pharmaceutical composition for prevention and treatment of gastric ulcer, containing extract of chicken or oyster |
| JP2005278569A (en) * | 2004-03-30 | 2005-10-13 | Yaizu Suisankagaku Industry Co Ltd | High octopine-containing fish and shellfish extract, method for producing the same, and food and drink containing the high octopine-containing fish and shellfish extract |
| WO2006077634A1 (en) * | 2005-01-19 | 2006-07-27 | Japan Clinic Co., Ltd. | Process for producing oyster meat extract |
| US20060246206A1 (en) | 2005-04-28 | 2006-11-02 | Mitsugu Watanabe | Preparation of oyster flesh extracts |
| CN108715600B (en) * | 2018-04-12 | 2021-07-13 | 中国科学院南海海洋研究所 | Oligopeptide for promoting proliferation and migration of intestinal mucosa epithelial cells and preparation method and application thereof |
-
1986
- 1986-04-28 JP JP61099247A patent/JPH0653053B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62257359A (en) | 1987-11-09 |
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