JPH0641949B2 - Bad breath test paper - Google Patents
Bad breath test paperInfo
- Publication number
- JPH0641949B2 JPH0641949B2 JP60255536A JP25553685A JPH0641949B2 JP H0641949 B2 JPH0641949 B2 JP H0641949B2 JP 60255536 A JP60255536 A JP 60255536A JP 25553685 A JP25553685 A JP 25553685A JP H0641949 B2 JPH0641949 B2 JP H0641949B2
- Authority
- JP
- Japan
- Prior art keywords
- halitosis
- bad breath
- test paper
- mercaptan
- yellow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/783—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour for analysing gases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/8483—Investigating reagent band
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Spectrometry And Color Measurement (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は，歯科医，歯科衛生士，あるいは一般人がきわ
めて容易に口臭の程度を測定し得る口臭試験紙に関す
る。TECHNICAL FIELD The present invention relates to a halitosis test paper which allows a dentist, a dental hygienist, or an ordinary person to very easily measure the degree of halitosis.
（従来の技術） 歯科分野においては，患者自身が自分の口臭を感じるこ
とが少ないために，医師は何らかの測定値（定量値）を
もってその患者自身の口臭度を知らせたいという要望を
もっている。さらに，歯科衛生士は、担当する歯のブラ
ッシング指導の結果である口腔内清浄度を簡便な方法も
しくは装置を用いて表示したいという要望をもってい
る。他方，自己口臭症といわれる自分の生理的口臭を病
的な悪臭と誤認している症例においては，そのような悪
臭のないことを測定値をもって示し説得する必要があ
る。個人的にも，社交上，自分の口臭を気にする人が増
加しており、口臭を客観的に，しかも容易に測定し得る
方法あるいは器具の開発が望まれている。(Prior Art) In the field of dentistry, since a patient rarely feels his or her own bad breath, a doctor desires to inform the patient of his or her own bad breath degree by some measurement value (quantitative value). Furthermore, the dental hygienist has a desire to display the oral cleanliness, which is the result of the brushing instruction of the tooth in charge, by using a simple method or device. On the other hand, in cases in which one's own physiological halitosis, called auto-halitosis, is mistaken for a pathological malodor, it is necessary to convince by showing the absence of such malodor with measured values. Personally, the number of people who are concerned about their bad breath is increasing due to social issues, and it is desired to develop a method or an instrument that can measure the bad breath objectively and easily.
口臭は口腔内の含硫タンパク質の腐敗により生じる硫化
水素およびメチルメルカプタンが主な原因物質（口臭の
９０％程度を占める）であることはよく知られている
（例えば，Oral Surg.,Vol.45,No.4,P.560〜567(197
8)。口臭の測定は，それゆえ，総硫黄量を測定すること
が望ましく，そのための最も一般的な方法として硫黄化
合物を酸化させて亜硫酸ガスにし，これを化学法・溶液
導電率法・赤外線ガス分析法および検知管法等にて測定
することが考えられる。しかし，これらの測定法はいづ
れも煩雑な酸化処理という前処理操作を必要とする。It is well known that hydrogen sulfide and methyl mercaptan produced by the decay of sulfur-containing proteins in the oral cavity are the main causative substances (accounting for about 90% of halitosis) (eg Oral Surg., Vol. 45). , No. 4, P. 560 ~ 567 (197
8). Therefore, it is desirable to measure the total amount of sulfur in the measurement of halitosis. The most common method for that is to oxidize sulfur compounds into sulfurous acid gas, which is then analyzed by chemical method, solution conductivity method, infrared gas analysis method. It is also possible to measure with the detector tube method. However, each of these measurement methods requires a complicated pretreatment operation called oxidation treatment.
このような前処理操作を要せず硫黄化合物を直接測定す
る方法としては，例えば，前出のOral Surg.に呼気中の
揮発性硫黄化合物の濃度をガスクロマトグラフィーを用
いて定量する方法が開示されている。ガスクロマトグラ
フィー分析はその感度・定量性において優れているが，
機器が高価である上に，試料（呼気）採取に特殊な器具
を要するため、個人的に使用することは困難であり，口
臭分析を必要とする歯科医や歯科衛生士にはなじまな
い。Arch.Oral Boil.Vol.9,P.39〜45(1964)には，唾液
を培養し培地中に生じる含硫化合物の濃度をガスクロマ
トグラフィー法や官能試験法により測定することが開示
されている。この方法は試料唾液の採取は容易であるが
培養に長時間を要するうえにガスクロマトグラフィーや
官能試験を採用するため迅速な口臭測定法とは言えな
い。また，特開昭57-135360号公報には，４、４′−ビ
スジメチルアミノジフェニルカルビトールが酸性下でカ
ルボニウムイオンを出して青色を示しチオール基と反応
して退色することを利用して，この化合物に唾液を加え
その退色度合いを比色法もしくは試験紙法で定量し，メ
ルカプタンを算出することが開示されている。この方法
は精度において優れるが約30分もの長時間を要し、かつ
比色計を必要とするため，歯科医や歯科衛生士のいる診
察室での口臭測定あるいは個人的な口臭測定には不適当
である。As a method for directly measuring a sulfur compound without requiring such a pretreatment operation, for example, a method of quantifying the concentration of a volatile sulfur compound in exhaled breath using gas chromatography is disclosed in the above-mentioned Oral Surg. Has been done. Gas chromatographic analysis is excellent in its sensitivity and quantitativeness,
Since the equipment is expensive and a special instrument is required to collect a sample (expired air), it is difficult to use it personally, and it is not familiar to dentists and dental hygienists who need halitosis analysis. Arch.Oral Boil.Vol.9, P.39-45 (1964) discloses that the concentration of sulfur-containing compounds produced in the culture medium of saliva is measured by gas chromatography or sensory test. There is. Although this method can easily collect saliva as a sample, it requires a long time for culturing, and since it employs gas chromatography and a sensory test, it cannot be said to be a rapid halitosis measuring method. Further, in Japanese Patent Application Laid-Open No. 57-135360, it is utilized that 4,4'-bisdimethylaminodiphenylcarbitol emits a carbonium ion under acidic condition to show blue color and reacts with a thiol group to discolor. It is disclosed that saliva is added to this compound and the degree of fading is quantified by a colorimetric method or a test paper method to calculate mercaptan. This method is excellent in accuracy, but it takes about 30 minutes and requires a colorimeter. Therefore, it is not suitable for measuring bad breath in a dentist's or dental hygienist's examination room or personal bad breath measurement. Appropriate.
さらに，口臭の主成分であるメチルメルカプタンと反応
する呈色試薬を担持体に含浸させて試験紙状にして口臭
を測定する試験紙法では、試験紙の色の濃淡変化を肉眼
で明確に識別することは困難である。通常，メチルメル
カプタンに対する呈色試薬としては，SH基の比色定量試
薬が利用され，具体的には，エールマン試薬である５，
５′−ジチオビス（２−ニトロ安息香酸）（DTNB）や，
その類似体である２，２′−ジチオジピリジン（2-PD
S），４，４′−ジチオピリジン，２，２′−ジチオビ
ス（５−ニトロピリジン），６，６′−ジチオニコチン
酸が挙げられる。また，ビス（４−ジメチルアミノフェ
ニル）メタノールから酸性pH（pH４〜6.5，望ましくはp
H５）で容易に変換される４，４′−ビスジメチルアミ
ノジフェニルカルボニウムイオンは青色を示し，メチル
メルカプタンとの反応で退色することから呈色試薬とし
て用いられる。Furthermore, in the test paper method in which the color reagent that reacts with methyl mercaptan, which is the main component of halitosis, is impregnated into the carrier to form a test paper, and the bad breath is measured, the change in shade of the test paper can be clearly identified with the naked eye. Is difficult to do. Usually, as a color reagent for methyl mercaptan, an SH group colorimetric assay reagent is used.
5'-dithiobis (2-nitrobenzoic acid) (DTNB),
Its analog, 2,2'-dithiodipyridine (2-PD
S), 4,4'-dithiopyridine, 2,2'-dithiobis (5-nitropyridine), 6,6'-dithionicotinic acid. Also, from bis (4-dimethylaminophenyl) methanol to acidic pH (pH 4-6.5, preferably p
The 4,4'-bisdimethylaminodiphenylcarbonium ion, which is easily converted in H5), shows a blue color and is used as a color reagent because it fades in the reaction with methyl mercaptan.
これらの試薬のうち，エールマン試薬およびその類似体
では，極大吸収波長部が紫外部〜近紫外部にあって黄色
を呈し，チオール類の比色法による定量に用いられる
が，その黄色の濃淡を肉眼で明確に識別することは非常
に困難である。ビス（４−ジメチルアミノフェニル）メ
タノールから得られる青色の濃淡も，同様に肉眼で識別
することは困難である。Among these reagents, Ehlmann's reagent and its analogues show a yellow color with a maximum absorption wavelength region in the ultraviolet to near-ultraviolet region and are used for the quantification of thiols by the colorimetric method. It is very difficult to identify clearly with the naked eye. The blue tint obtained from bis (4-dimethylaminophenyl) methanol is likewise difficult to identify with the naked eye.
（発明が解決しようとする問題点） 本発明は上記従来技術の問題点を解決するものであり，
その目的とするところは，口臭の程度をきわめて容易に
かつ短時間で，客観的かつ高精度で測定し得る口臭試験
紙を提供することにある。(Problems to be Solved by the Invention) The present invention solves the above-mentioned problems of the prior art.
It is an object of the invention to provide a halitosis test strip that can measure the degree of halitosis objectively and with high accuracy very easily and in a short time.
（問題点を解決するための手段） 上述したように，肉眼による色の濃淡の識別はきわめて
困難であるが，色調の変化は肉眼によっても正確に識別
することができる。例えば，一般の尿検査用試験紙で
は，色調の変化によって半定量を行っている。本発明は
このような知見に基づいて完成された。(Means for Solving Problems) As described above, it is extremely difficult to distinguish the shade of color with the naked eye, but the change in color tone can be accurately distinguished with the naked eye. For example, in general urine test paper, semi-quantitative measurement is performed by changing the color tone. The present invention has been completed based on these findings.
本発明の口臭試験紙は，口臭の成分であるメルカプタン
と反応して変色する，ビス（４−ジメチルアミノフェニ
ル）メタノールおよびビス（４−ジエチルアミノフェニ
ル）メタノールのうちいずれか一方の試薬と，前記試薬
および色素を含浸させた担持体とを具備し，試料唾液の
添着による色調の変化から口臭の程度を判定する構成と
し，そのことにより上記目的が達成される。例えば，口
臭の主成分であるメルカプタンと反応して青色が褪色す
る前記試薬と、あらかじめ黄色の色素とを，例えば濾紙
のような担持体に含浸させた本発明の口臭試験紙は，メ
ルカプタンとの反応により緑色から黄色へと変色する。
したがって，既知濃度のメルカプタンに対する試験紙の
呈色状態を調べて，標準比色表を作成しておけば，試料
を添加してから所定時間経過後の試験紙の呈色状態を該
標準比色表と対比することにより，試料中のメルカプタ
ン濃度を知ることができ，口臭の程度の客観的な判定が
可能になる。The halitosis test paper of the present invention is a reagent of any one of bis (4-dimethylaminophenyl) methanol and bis (4-diethylaminophenyl) methanol, which changes color by reacting with mercaptan which is a component of halitosis, and the above-mentioned reagent. And a carrier impregnated with a dye, and the constitution is such that the degree of bad breath is judged from the change in color tone due to the attachment of the sample saliva, whereby the above object is achieved. For example, a halitosis test paper of the present invention obtained by impregnating a carrier such as a filter paper with the above-mentioned reagent that reacts with mercaptan which is a main component of halitosis and fading blue color, and a yellow pigment in advance is The reaction changes the color from green to yellow.
Therefore, if a standard colorimetric table is prepared by examining the coloration state of the test paper for a known concentration of mercaptan, it is possible to determine the coloration state of the test paper after the lapse of a predetermined time after adding the sample. By comparing with the table, it is possible to know the mercaptan concentration in the sample and to objectively judge the degree of bad breath.
（実施例） 以下に本発明の実施例について説明する。(Examples) Examples of the present invention will be described below.
実施例１ ＜口臭試験紙の調製＞ 黄色色素として不透明水彩絵具（日本ホルバイン社製，
パーマネントイエローレモンNo.631）50mgを水40mgに懸
濁し，濾紙（東洋濾紙No.131）に含浸させた後に風乾す
る。次に，ビス（４−ジメチルアミノフェニル）メタノ
ール（別名，４，４′−ビス（ジメチルアミノ）ベンズ
ヒドロール，同仁化学社製）6.4mgを10ｍのアセトン
溶液に溶かし，その溶液２ｍを，0.1Ｍクエン酸緩衝
液（pH5.01）に滴下して精密に10ｍとし，青色の試薬
液を得る。該青色の試薬液を，上述の黄色色素を含浸さ
せた濾紙に含浸させ，凍結乾燥させた。これにより緑色
の口臭試験紙が得られた。Example 1 <Preparation of bad breath test paper> Opaque watercolor paint as a yellow pigment (manufactured by Nippon Holbein Co.,
50 mg of Permanent Yellow Lemon No. 631) is suspended in 40 mg of water, impregnated with filter paper (Toyo Filter Paper No. 131), and then air dried. Next, 6.4 mg of bis (4-dimethylaminophenyl) methanol (also known as 4,4'-bis (dimethylamino) benzhydrol, manufactured by Dojindo Co., Ltd.) was dissolved in 10 m of acetone solution, and 2 m of the solution was added to 0.1 ml. Drop to M citrate buffer (pH 5.01) to make exactly 10 m to obtain a blue reagent solution. The blue reagent solution was impregnated into the filter paper impregnated with the above-mentioned yellow dye and freeze-dried. As a result, a green bad breath test paper was obtained.
＜試験片の調製＞ 得られた口臭試験紙を６×10mmに切断し，両面粘着テー
プにより６×60mmの白色プラスチック片に固定して試験
片とした。<Preparation of test piece> The obtained bad breath test paper was cut into a piece of 6 x 10 mm and fixed on a white plastic piece of 6 x 60 mm with a double-sided adhesive tape to give a test piece.
＜測定＞ 該試験片に濃度既知のメルカプタン溶液を滴下し，試験
片の色調の変化を，色彩色差計（ミノルタ社製CR100
型）によって調べた。その結果を第１図に示す。メルカ
プタン濃度が大きくなるほど，試験片は緑色（ａ＊座標
の負の値が大きくなれば緑色に近くなる）から，黄色
（ｂ＊座標の正の値が大きくなれば黄色に近くなる）に
移行し，また時間の経過に伴って，同様に緑色から黄色
へと移行する。メルカプタン濃度および反応時間と色調
の変化との相関係数は0.962であった。したがって，反
応開始から所定時間後の色調の変化から，メルカプタン
の半定量が可能である。<Measurement> A mercaptan solution of known concentration was dropped onto the test piece, and the change in color tone of the test piece was measured by a color difference meter (CR100 manufactured by Minolta Co., Ltd.).
Type). The results are shown in FIG. As the mercaptan concentration increases, the test piece shifts from green (closer to green when the negative value of the a * coordinate increases) to yellow (closer to yellow when the positive value of the b * coordinate increases). , Also, with the passage of time, it similarly changes from green to yellow. The correlation coefficient between mercaptan concentration and reaction time and change in color tone was 0.962. Therefore, semi-quantitative determination of mercaptan is possible from the change in color tone after a predetermined time from the start of the reaction.
実施例２ ＜口臭試験紙の調製＞ 実施例１で調製した青色の試薬液を濾紙（東洋濾紙社
製，No.131）に含浸させた後，凍結乾燥する。メチルレ
ッド（和光純薬社製）50mgをエタノール100ｍに溶か
し，その上澄液を，上記濾紙に含浸させて風乾する。こ
れにより紫色の口臭試験紙が得られた。Example 2 <Preparation of halitosis test paper> Filter paper (No. 131, manufactured by Toyo Roshi Kaisha, Ltd.) is impregnated with the blue reagent solution prepared in Example 1 and then freeze-dried. 50 mg of methyl red (manufactured by Wako Pure Chemical Industries, Ltd.) is dissolved in 100 m of ethanol, and the supernatant is impregnated in the above filter paper and air dried. As a result, a purple halitosis test paper was obtained.
＜試験片の調整＞ 該口臭試験紙を６×10mmの細片に切断し，両面テープに
て白色プラスチック片に固定して試験片とした。<Adjustment of test piece> The bad breath test paper was cut into 6 x 10 mm strips and fixed to a white plastic piece with a double-sided tape to give a test piece.
＜測定＞ 該試験片に20μの濃度既知のメルカプタンを滴下し，
30〜60秒後の色調を調べた。その結果を下表に示す。<Measurement> 20 μm of a known mercaptan was dropped on the test piece,
The color tone after 30 to 60 seconds was examined. The results are shown in the table below.
メルカプタンが含まれていない紫色の色調を０と判定
し，メルカプタン濃度がそれぞれ５，10,20ppmの場合の
各色調，紫赤色，赤紫色，赤色をそれぞれ±，＋，と
し，さらに各メルカプタン濃度に対応させてスコアを，
それぞれ０，１，２，３とした。 The purple color tone that does not contain mercaptan is judged as 0, and each color tone when the mercaptan concentration is 5, 10, 20 ppm, purple red, red purple, and red are ±, +, respectively, and further, for each mercaptan concentration Corresponding score
It was set to 0, 1, 2, and 3, respectively.
次に，上記試験片に唾液を滴下して，その色調からスコ
アを認定すると共に，口臭の官能評価を行った。その結
果を第２図に示す。第２図より明らかなように，上記試
験片によるスコアと口臭官能評価とは良好な相関関係を
示し，口臭を客観的に評価し得る。Next, saliva was dripped on the above-mentioned test piece, the score was recognized from the color tone, and the sensory evaluation of bad breath was performed. The results are shown in FIG. As is clear from FIG. 2, the score of the test piece and the sensory evaluation of bad breath show a good correlation, and the bad breath can be objectively evaluated.
口臭官能評価は以下の基準に基づいて行われた。The halitosis sensory evaluation was performed based on the following criteria.
口臭官能評価 ０：無〜低臭；低度の許容できる臭い。Halitosis sensory evaluation 0: No to low odor; low acceptable odor.
１：低〜中低度臭；中低度の口臭。大部分は許容性，一
部不快臭有り。1: Low to moderately low odor; moderate to low bad breath. Mostly tolerable with some unpleasant odor.
２：中〜強い悪臭，中および強い口臭；大部分は不快
臭。2: Moderate to strong bad odor, medium and strong bad breath; mostly unpleasant odor.
３：強い悪臭；不快な（強い）口臭。3: Strong offensive odor; unpleasant (strong) bad breath.
実施例３ ＜ビス（４−ジエチルアミノフェニル）メタノールの調
製＞ ４，４′−ビス（ジエチルアミノ）ベンゾフェノン（ア
ルドリッチ社製）1.6ｇを乾燥エーテル70ｍに溶か
し，氷冷下で攪拌しながら，水素化リチウムアルミニウ
ム（メルク社製）0.1ｇを徐々に加える。その後，室温
にて攪拌し，１時間後，シリカゲル薄層クロマトグラフ
ィー（展開溶媒，ヘキサン：酢酸エチル＝５：１V/V）
にて，出発原料の消失を確認後，氷片を少量加える。エ
ーテルにて希釈（30ｍ）した後，エーテル層を無水硫
酸ナトリウムで脱水し，次いで濾別し，濾液をチッソ気
流下に濃縮すると，白色結晶が得られる（収量1.53ｇ，
95％）。これをヘキサン−エーテルで再結晶する（融点
79〜80℃（文献値78.5〜79℃））。Example 3 <Preparation of bis (4-diethylaminophenyl) methanol> 1.6 g of 4,4′-bis (diethylamino) benzophenone (manufactured by Aldrich) was dissolved in 70 m of dry ether, and lithium hydride was stirred under ice cooling. Gradually add 0.1 g of aluminum (Merck). Then, the mixture was stirred at room temperature, and after 1 hour, silica gel thin layer chromatography (developing solvent, hexane: ethyl acetate = 5: 1V / V).
After confirming the disappearance of the starting material, add a small amount of ice chips. After diluting with ether (30 m), the ether layer was dried over anhydrous sodium sulfate, then filtered off, and the filtrate was concentrated under a nitrogen stream to give white crystals (yield 1.53 g,
95%). This is recrystallized from hexane-ether (melting point)
79-80 ° C (literature value 78.5-79 ° C)).
＜口臭試験紙の調製＞ 上述のようにして得られたビス（４−ジエチルアミノフ
ェニル）メタノール7.7mgを10ｍのアセトン溶液に溶
かし，その溶液２ｍを，ポリエチレングリコール20,0
00（和光純薬社製）40mgを溶かした0.1Ｍクエン酸緩衝
液（pH6.0）８ｍに滴下し，青色の試薬液を精密に10
ｍ得た。該青色の試薬液を実施例１に用いたものと同
様の黄色濾紙に含浸し，凍結乾燥することにより緑黄色
の口臭試験紙が得られた。<Preparation of halitosis test paper> 7.7 mg of bis (4-diethylaminophenyl) methanol obtained as described above was dissolved in 10 m of acetone solution, and 2 m of the solution was added to polyethylene glycol 20,0.
00 (manufactured by Wako Pure Chemical Industries, Ltd.) 40 mg was dissolved in 0.1 M citrate buffer solution (pH 6.0) 8 m, and the blue reagent solution was precisely added to 10 m.
I got m. A yellow filter paper similar to that used in Example 1 was impregnated with the blue reagent solution and freeze-dried to obtain a green-yellow halitosis test paper.
＜試験片の調製＞ 緑黄色の口臭試験紙を６×10mmに切断し，両面粘着テー
プにより白色プラスチック片に固定した。<Preparation of test piece> A green-yellow halitosis test paper was cut into 6 x 10 mm and fixed to a white plastic piece with a double-sided adhesive tape.
＜測定＞ 実施例２と同様に，該試験片に濃度既知のメルカプタン
溶液20μを滴下し，20〜30秒後の色調を調べた。メル
カプタン濃度が０ppmでは，色調の変化はほとんどな
く，５ppmでは幾分黄色に近くなり，10ppmでは20秒後に
黄色になった。メルカプタン濃度が10ppmでは前述の表
に示すように，スコアは２となる。第２図ではスコア２
において，口臭官能評価が２（大部分が不快臭）であ
り，したがって，この実施例３における口臭試験紙で
は，不快臭の強い場合を，緑黄色から黄色への色調の変
化により明確に検知できる。<Measurement> In the same manner as in Example 2, 20 μ of a mercaptan solution having a known concentration was dropped on the test piece, and the color tone after 20 to 30 seconds was examined. At a mercaptan concentration of 0 ppm, there was almost no change in color tone, at 5 ppm it became somewhat yellow, and at 10 ppm it became yellow after 20 seconds. When the mercaptan concentration is 10 ppm, the score is 2 as shown in the above table. Score 2 in Figure 2
In 2), the halitosis sensory evaluation is 2 (most of which are unpleasant odors). Therefore, with the halitosis test paper in Example 3, the case of strong unpleasant odor can be clearly detected by the change in color tone from green yellow to yellow.
（発明の効果） 本発明によれば，このように，微量の試料唾液を用い
て，口臭の程度をきわめて容易に，しかも短時間で測定
することができる。口臭の程度を，色調の変化によって
検出し得るので，誰にでも口臭の程度を客観的に判定し
得る。(Effect of the Invention) According to the present invention, the degree of halitosis can be measured very easily and in a short time by using a small amount of sample saliva. Since the degree of bad breath can be detected by the change in color tone, anyone can objectively judge the degree of bad breath.
第１図はメルカプタン濃度および反応時間と色調の変化
との相関を示すグラフ，第２図は口臭官能評価とスコア
との相関を示すグラフである。FIG. 1 is a graph showing the correlation between mercaptan concentration and reaction time and the change in color tone, and FIG. 2 is a graph showing the correlation between halitosis sensory evaluation and score.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｇ０１Ｎ 31/22 Ｃ 7906−2Ｊ 33/497 Ｂ 7055−2Ｊ 33/52 Ｂ 7055−2Ｊ ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Internal reference number FI Technical display location G01N 31/22 C 7906-2J 33/497 B 7055-2J 33/52 B 7055-2J
Claims (1)
変色する，ビス（４−ジメチルアミノフェニル）メタノ
ールおよびビス（４−ジエチルアミノフェニル）メタノ
ールのうちいずれか一方の試薬と，前記試薬および色素
を含浸させた担持体とを具備し，試料唾液の添着による
色調の変化から口臭の程度を判定する構成とした口臭試
験紙。1. A reagent of any one of bis (4-dimethylaminophenyl) methanol and bis (4-diethylaminophenyl) methanol, which changes color by reacting with mercaptan, which is a component of halitosis, and the reagent and the dye. A halitosis test paper comprising an impregnated carrier and configured to judge the degree of halitosis based on a change in color tone due to the addition of sample saliva.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60255536A JPH0641949B2 (en) | 1985-11-14 | 1985-11-14 | Bad breath test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60255536A JPH0641949B2 (en) | 1985-11-14 | 1985-11-14 | Bad breath test paper |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62115364A JPS62115364A (en) | 1987-05-27 |
| JPH0641949B2 true JPH0641949B2 (en) | 1994-06-01 |
Family
ID=17280093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60255536A Expired - Lifetime JPH0641949B2 (en) | 1985-11-14 | 1985-11-14 | Bad breath test paper |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0641949B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002066976A1 (en) * | 2001-02-16 | 2002-08-29 | Selena Mazuran | Personal good/bad breath tester |
| US7837663B2 (en) * | 2003-10-16 | 2010-11-23 | Kimberly-Clark Worldwide, Inc. | Odor controlling article including a visual indicating device for monitoring odor absorption |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS447598Y1 (en) * | 1966-05-12 | 1969-03-22 |
-
1985
- 1985-11-14 JP JP60255536A patent/JPH0641949B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62115364A (en) | 1987-05-27 |
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