JPH0638932B2 - Centrifugal type mouth use container - Google Patents

Description

TECHNICAL FIELD The present invention relates to a centrifugal filtration container for filtering a trace amount of liquid by centrifugal force, which is used for pretreatment such as clinical analysis and chemical analysis. The present invention relates to a centrifugal filtration container that can be used for concentration, desalting, buffer exchange, etc. of high molecular weight substances.

(Prior Art) Conventionally, there is a micro-separation device (Japanese Patent Laid-Open No. 60-44004) that uses a centrifuge equipped with an angle type rotor for the purpose of concentrating and recovering a polymer in a sample solution. There was no microseparator that could utilize a centrifuge equipped with a mold rotor. This is because the dead stopper does not work in the case of a swing type rotor.

Furthermore, when a part of the filtrate comes into contact with the part where the filter tray mounting part and the filter tray are fitted together, it may rise due to the capillary phenomenon of the fitted part and leak to the outside, resulting in sample loss. It is not desirable.

(Object of the Invention) The present invention provides a centrifugal filtration container capable of collecting a concentrate regardless of whether a swing type rotor or an angle type rotor is used. It is intended for a centrifugal filtration container that does not leak liquid.

(Structure of the Invention) Hereinafter, the present invention will be described in detail with reference to the drawings.

FIG. 1 is a schematic view of the centrifugal filtration container of the present invention.

In Fig. 1, the centrifugal filtration container is a sample reservoir (1).
And a separation membrane (2) having an inclination angle fixed to the lower part of the sample reservoir (1), a support portion (3) of the separation membrane (2) and the support portion (3)
The portion consisting of the liquid solution pan mounting portion (4) provided in the lower part and the liquid solution pan (5) which can be fitted into the liquid solution pan mounting portion (4) are integrated and separated. The inclination angle of the membrane (2) is 25
° to 45 °, the bottom of the inclined portion has a dead stop portion.

If the tilt angle is less than 25 °, the amount of the concentrated solution to be recovered is small, and if it exceeds 45 °, the housing is not easily formed, which is not preferable. In addition, the dead stop portion has a correlation with the inclination angle, and it is preferably 20 to 30% of the area of the support portion, which cannot be specified depending on the amount of the concentrated solution to be recovered but has almost no influence on the filtration rate.

Next, one embodiment of the support part (3) will be described.

FIG. 2 is an enlarged plan view of the support part (3). In FIG. 2, the support part (3) is configured by planarly integrating a liquid passage part (6), a membrane support part (7) and a dead stop part (8) also serving as a membrane support part. It has been done.

The membrane supporting part (7) and the liquid passage part (6) are correlated with each other, and it does not matter even if an arbitrary shape is formed, but since the dead stop part is a part for holding the concentrated solution, it is a supporter. Department
It is formed as a blind from one end of (3) and forms the bottom of the inclined part.

A dead stop part is formed by supporting the separation membrane (2) of the support part (3) configured as described above.

Although the concentrated solution obtained by the present invention is not shown in FIG. 1, a concentrated liquid recovery cap is fitted on the upper part of the sample reservoir (1) and the concentrated liquid receiving tray (5) is attached to the concentrated liquid receiving tray. After removing from the site (4), it can be recovered by centrifuging the concentrated solution recovery cap as the lower part.

Further, the shape of the liquid storage pan mounting portion in which the liquid does not leak in the centrifugal filtration container will be described.

FIG. 3 is a cross-sectional view of a part where the support part (3) of the separation membrane and the liquid solution tray mounting part (4) provided in the lower part of the support part are integrated.

In FIG. 3, the thickness of the portion (4) for mounting the liquid tray is the thickness thereof.
(t) and the outer diameter (D) of the cylinder have the following relationship. Further, the outer diameter (D) is 6 mmD20 mm, and the angle α with respect to the central axis of the outer cutout portion of the bottom is 35 ° α55 °.

(Effects of the Invention) In the centrifugal filtration container of the present invention, the membrane support part has an inclination angle and a part of the bottom part thereof is blind, so that a certain amount of the concentrated liquid is separated into a separation membrane. It can be left on top and the concentrate can be easily recovered. In addition, since the separation membrane support portion of the container is inclined, when the centrifugal separator equipped with the swing type rotor is used, the concentrated liquid can be retained regardless of the insertion direction of the container. Even when a centrifuge equipped with an angle rotor is used, the concentrated liquid can be retained similarly.

Furthermore, since leakage of the filtered liquid does not occur, if necessary,
It is possible to effectively use the filtrate.

(Example 1) A centrifugal filtration container as shown in FIG. 1 in which an asymmetric ultrafiltration membrane of regenerated cellulose having a cut-off molecular weight of 3 × 10 ⁴ was attached at 30 ° and the dead stop portion was 25%. Was produced. Except for the separation membrane, all were made of polymethylmethacrylate resin. Serum albumin (hereinafter abbreviated as BSA) (1 mg / m) (1 m) was placed in a sample reservoir as a sample solution, and centrifugally concentrated at 1000 G for 30 minutes with a swing-type centrifugal rotor to measure the amount of the concentrated solution. After the concentration is completed, attach the concentrate collecting cap to the upper part of the sample reservoir, remove the filtrate tray, invert the centrifugal filtration container, centrifuge at 1000 G for 5 minutes with a swing type centrifugal rotor, and concentrate. The liquid was collected. The results are shown in Table 1.

(Example 2) A centrifugal filtration container similar to that in Example 1 was manufactured, and 1 m of BSA (1 mg / m) was put in a sample reservoir as a sample, and the sample was stored in a 45 ° angle rotor. When the centrifugal filtration container was inserted into the angle rotor, the container was inserted so that the film surface was horizontal, and the mixture was centrifugally concentrated at 1700 G for 30 minutes, and the amount of the concentrated liquid was measured. After the concentration was completed, the centrifugal filtration container was reversed as in Example 1, and the angle centrifugal rotor was used to 170
The concentrate was collected by centrifugation at 0 G for 5 minutes. The results are shown in Table 2.

[Brief description of drawings]

FIG. 1 is a schematic view of a centrifugal filtration container of the present invention, FIG. 2 is an enlarged plan view of a support portion of a separation membrane, and FIG. 3 is an integrated support portion and a filter tray. It is sectional drawing with a mounting site. (1) ... Sample reservoir (2) ... Separation membrane (3) ... Support part (4) ... Battery saucer mounting part (5) ... Battery saucer (6) ... Battery passage section (7) ... Membrane Support part (8) ... Dead stop part

Claims (3)

[Claims] 1. A portion comprising a sample reservoir, a separation membrane fixed to the lower portion of the sample reservoir, a support portion of the separation membrane, and a liquid saucer mounting portion provided at the lower portion of the support portion; In a centrifugal filtration container in which a liquid tray that can be fitted to a liquid tray mounting portion is integrated, the support portion has an inclination angle of 25 to 45 °, and the bottom of the inclined portion is dead. A centrifugal type filtration container having a stop part. 2. The dead stop portion has a support portion area of 20 to 20.
The centrifugal filtration container according to claim (1), which has a content of 30%. 3. A sample reservoir, a separation membrane fixed to the lower part of the sample reservoir, a part comprising a support part of the separation membrane and a solution receiving pan mounting part provided under the support part, and the solution. In a centrifugal filtration container in which a liquid tray which can be fitted to a tray mounting portion is integrated, the support portion has an inclination angle of 25 to 45 °, and the bottom of the inclined portion is a dead stop. And the outer diameter of the cylinder (D) and the thickness (t) of the portion for mounting the liquid tray is configured by the following equation, Further, the centrifugal filtration container is characterized in that the outer diameter (D) is 6 mmD20 mm, and the angle α with respect to the central axis of the outer cutout portion of the bottom is 35 ° α55 °.