JPH06341987A - Detecting device for methamphethamine or its derivative - Google Patents

Abstract

PURPOSE:To provide a device to simply and quickly detect methamphethamine or its derivative at high sensitivity. CONSTITUTION:Sample liquid, antimethamphethamine antibody liquid and fluorescent coloring matter are severally stored in storing containers 1, 2, 3, and valves 4, 5, 6 and pumps 7, 8, 9 ar controlled to inject a fixed quantity of each liquid in order into a reaction vessel 11. The quantity of methamphetamine in the sample liquid is measured from a change in the fluorescence intensity of the sample liquid before and after the injection of the antimethamphethamine antibody liquid.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a detector for methamphetamine or a derivative thereof, which is used in medical care, police, customs duties and the like.

[0002]

BACKGROUND OF THE INVENTION Methamphetamine or its derivatives are the main components of stimulants, and there is a demand for development of a technique for detecting them easily and quickly. Generally, as a method for detecting these methamphetamine or a derivative thereof, gas chromatography or mass spectrometry in which a powder sample or a solution sample is introduced into a detection device for detection, and a solution sample is introduced in the device for detection. Liquid chromatography, a flow type or manual batch type detection method using an antigen-antibody reaction, an enzyme immunoassay method using a polyclonal antibody, and the like are performed.

[0003]

However, as a sample to be investigated whether or not these methamphetamines or their derivatives are contained, a spill on the floor of a suspect's house where a suspected stimulant was suspected was searched. Those collected by a dust collector are included. The sample thus collected contains many impurities such as dust and dust. Therefore, in gas chromatography, liquid chromatography, and mass spectrometry in which a sample is directly or in solution to perform detection, it is difficult to accurately detect the presence or absence of methamphetamine or the amount of methamphetamine due to the influence of impurities contained in the sample. there were. Further, generally, in each of these detection methods, a water circulation type thermostatic device is used in order to keep the temperature constant, so that the use in cold regions is restricted and maintenance is required.

In the flow type detection method, when an antigen-antibody reaction is carried out, a reagent and a sample are continuously supplied into a tubular reaction vessel (cell) and mixed diffusively, so that the sensitivity is lowered by dilution. Occurs. In the manual batch detection method, the liquefied sample, antibody solution, and dye solution are manually pipetted into the reaction container, and fluorescence is detected after stirring.Therefore, wash the reaction container etc. for each detection. Work efficiency was low. Furthermore, since the enzyme immunoassay method uses a polyclonal antibody, it has been difficult to achieve high sensitivity.

The present invention has been made in order to solve the above problems, and removes the influence of impurities contained in a sample, and is capable of detecting methamphetamine or a derivative thereof with high sensitivity and easy maintenance. The purpose is to provide a device.

[0006]

In order to achieve the above object, the detection apparatus for methamphetamine or a derivative thereof according to the present invention comprises a powder sample dissolved in a fluorescent dye solution having a methamphetamine structure, and an anti-methamphetamine antibody reacted with the sample solution. A detector for methamphetamine or a derivative thereof for measuring the presence or the amount of methamphetamine or a derivative thereof in the sample liquid from the change in fluorescence intensity of the sample liquid, wherein the sample solution, the fluorescent dye solution and the anti-methamphetamine antibody solution are A storage container for storing each, a reaction container for injecting and reacting the sample solution and the antibody solution, a fluorescence detection means for detecting the fluorescence intensity of the solution to be measured in the reaction container, and from each storage container A predetermined amount of the sample solution, the fluorescent dye and the anti-methamphetamine Characterized by comprising a supply means for supplying antibodies to each said reaction vessel.

In the above construction, it is preferable that the supply means is composed of a syringe type pump and a valve. Further, the reaction container is preferably a rectangular cell having an inlet and an air vent hole in the upper part and an exhaust nozzle in the lower part. Furthermore, it is preferable that the reaction container has a stirring means driven by a magnetic force for stirring the sample liquid, the fluorescent dye and the anti-methamphetamine antibody inside. Further, it is preferable that the reaction container is provided with a thermostatic device using a Peltier device. Further, it is preferable that at least the storage container and the supply means are provided inside the air-conditioning type thermostatic device.

[0008]

[Function] When a pseudo-antigen composed of a fluorescent dye having a methamphetamine structure is reacted with an anti-methamphetamine antibody, the pseudo-antigen and the antibody are bound to each other, and the fluorescence intensity (for example, excitation wavelength : 600n
m, fluorescence wavelength: 660 nm). When a sample solution is added to this, methamphetamine or its derivative in the sample solution binds to the antibody, and the fluorescent dye that is the pseudo-antigen bound to the antibody separates. Since the fluorescent dye becomes independent again, the fluorescence intensity decreases. Utilizing this phenomenon, the change in fluorescence intensity of the fluorescent dye is detected, and the presence or absence of methamphetamine or its derivative in the sample solution and its content, if present, are detected. Further, since a monoclonal antibody having high specificity for methamphetamine or its derivative is used, it reacts sensitively to methamphetamine or its derivative in the sample, and the sensitivity is improved.

Since a predetermined amount of the sample solution, the fluorescent dye and the anti-methamphetamine antibody are supplied from the storage container to the reaction container by the supply means and the change in the fluorescence intensity of the fluorescent dye is detected, the methamphetamine or the methamphetamine in the sample solution is detected. The detection of the derivative becomes easy. Further, since the inlet and the air vent hole were provided in the upper part of the reaction vessel and the discharge nozzle was provided in the lower part, the methamphetamine in different samples could be continuously changed by controlling the opening and closing of the supply means and the discharge nozzle. Alternatively, it becomes possible to detect the presence or the amount of the derivative. Further, since the reaction container is provided with a stirring means driven by a magnetic force for stirring the sample solution, the fluorescent dye and the anti-methamphetamine antibody, the sample solution and the reagent can be easily stirred, and the process can be performed easily. The time required is reduced. Further, since the reaction vessel is provided with a thermostatic device using a Peltier device, the detection work is performed under a constant temperature environment. further,
Since at least the storage container and the supply means are provided inside the air-conditioning type thermostatic device, it is possible to detect methamphetamine and its derivative under any temperature environment.

[0010]

【Example】

<First Embodiment> A detector for methamphetamine and a derivative thereof according to the present invention will be described with reference to FIGS. 1, 2, 3 and 4 showing a preferred first embodiment thereof. FIG. 1 is a diagram showing a structural formula of a fluorescent dye having a methamphetamine structure, which is used throughout each embodiment of the device according to the present invention, FIG.
Is a block diagram showing the configuration of the apparatus according to the first embodiment, and FIG. 3 is a perspective view showing the configuration of the reaction container.

In FIG. 2, the first storage container 1 contains a sample solution, the second storage container 2 contains an anti-methamphetamine antibody solution, and the third storage container 3 contains fluorescence having a methamphetamine structure as shown in FIG. Stores the pigment liquids respectively. A first pump 7 and a first valve 4 are connected to the first storage container 1. Similarly, the second storage container 2
Has a second pump 8 and a second valve 5, and a third
The third storage container 3 has a third pump 9 and a third valve 6
Are connected respectively. Further, the first valve 4 and the third valve 6 are each connected to the switching valve 10. The switching valve 10 and the second valve 5 are connected to the upper portion 11a of the reaction vessel 11, respectively. The discharge nozzle 11b at the bottom of the reaction vessel 11 is a discharge valve 1
5 and the discharge pump 13 are connected to the waste liquid container 14. Further, the reaction container 11 is provided with a fluorescence detector 12. Various types of pumps known for each pump can be used, but in particular the first, second and third pumps 7, 8 and 9 provide precise control over the amount of liquid to be maintained and supplied. From the viewpoint of obtaining it, it is preferable to use a syringe pump.

The reaction vessel 11 is a so-called prismatic cell having a rectangular cross section as shown in FIG. 3, and a switching valve 10 and a connecting pipe 11 from the second valve 5 are provided in an upper portion 11a.
c and an air vent hole 11d are provided. In general, the shape of the reaction vessel is not limited to the one having a rectangular cross section, and the one having a shape other than the rectangular cross section such as a circular cross section can also be used. However, the use of a shape other than the rectangular cross section is not suitable because the light scattering phenomenon occurs when measuring the fluorescence intensity. In addition, when injecting a liquid such as a sample liquid, it is preferable to provide an air vent hole inside the reaction container 11 so as not to apply a back pressure, in order to accurately control the injection amount.

<Second Embodiment> Next, a second preferred embodiment of the methamphetamine and its derivative detecting apparatus of the present invention will be described with reference to FIG. FIG. 5 is a block diagram showing the configuration of the device according to the second embodiment. The members given the same numbers as those in the first embodiment are substantially the same, and the description thereof will be omitted. In the second embodiment, in addition to the apparatus according to the first embodiment, a stirring member 17 is provided inside the reaction container 11, and further the stirring member 17 is provided outside the reaction container 11.
A drive source 16 for driving the motor is provided. The stirring member 17 and the driving source 16 are not directly mechanically connected to each other, and the stirring member 17 is rotated by the magnetic force generated by the driving source 16. As a result, the reaction between the sample liquid and the anti-methamphetamine antibody inside the reaction container 11 is promoted. In addition, a stirring device having a structure other than this can also be used. However, since the reaction container 11 used in each of the embodiments of the present invention has a small internal volume of, for example, about 2 ml, it is difficult to use a stirring device having a complicated structure or a large stirring device. Therefore, the above-mentioned magnetic drive system, which has a simple structure and in which the rotary shaft does not directly penetrate the container wall, is excellent.

<Third Embodiment> Next, a preferred third embodiment of the methamphetamine and its derivative detecting apparatus of the present invention will be described with reference to FIGS. 6 and 7. FIG. 6 is a block diagram showing the arrangement of an apparatus according to the third embodiment, (a) of FIG.
And (b) are a front view and a side view, respectively, showing the configuration of an embodiment of a thermostatic device. The members given the same reference numerals as those in the first and second embodiments are substantially the same, and the description thereof will be omitted. In the third embodiment,
In addition to the first and second embodiments, the fluorescence detector 12
A thermostatic device 18 using a Peltier device is provided near the reaction container 11 of FIG.
Is provided inside the temperature-controlled room 20 under a constant temperature environment. As shown in FIG. 7, the rectangular reaction vessel 11 is fixed to one surface of an aluminum block 31,
The Peltier element 32 is fixed to the other surface of the block 31. Furthermore, fins 33 made of aluminum are attached to the surface opposite to the Peltier element 32. As described above, by mounting the Peltier element 32 on the reaction vessel 11 via the block 31 having a large heat capacity, when the sample liquid or the like is injected into the reaction vessel 11 or the Peltier element 32.
The sudden temperature change when the heat is generated is alleviated. Further, since the entire apparatus is provided inside the temperature-controlled room 20, stable detection can be performed regardless of environmental conditions such as cold regions and warm regions.

<Fourth Embodiment> Next, a preferred fourth embodiment of the methamphetamine and its derivative detecting apparatus of the present invention will be described with reference to FIG. FIG. 8 is a block diagram showing the arrangement of an apparatus according to the fourth embodiment.
The members with the same numbers as in the embodiment are substantially the same, and the description thereof will be omitted. In the fourth embodiment, a computer 21 is provided as a control device, whereby each valve 4, 5, 6 and 10 and each pump 7, 8, 9 are provided.
It is configured such that the control of 13 and 13 is automatically performed. As a result, methamphetamine or a derivative thereof can be easily detected by a person who is unfamiliar with the handling of the device without requiring any special knowledge.

<Operation in Each Example> Next, the operation of the methamphetamine or its derivative detecting apparatus in each of the above-mentioned examples will be described. As the sample solution, when actually used, a sample collected by a dust collector or the like is dissolved with a dye solution having a predetermined concentration. Here, in order to obtain a characteristic curve as shown in FIG. Of methamphetamine (1 × 10 ⁻¹² to 1 × 10 ⁻⁴ M (mol /
l)) is used by dissolving a known amount of methamphetamine powder with 6 × 10 ⁻⁷ M dye solution.

First, the first pump 7 for sample liquid is driven to suck the sample liquid from the first storage container 1 into it. Next, the first valve 4 for the sample solution and the switching valve 10 are switched to switch the first pump 7 to the reaction vessel 11
1.10 ml of the sample solution is injected into the inside of the container. here,
Desirably, the fluorescence intensity of the sample liquid is measured at an excitation wavelength of 600 nm and a fluorescence wavelength of 660 nm while rotating the stirring member 17 shown in FIG. After that, the second pump 8 for the antibody solution is driven so that the second storage container 2
0.08m of 1 × 10 ^-5 M antibody solution through the valve 5 of
l Aspirate. Further, the second valve 5 is switched, and the antibody solution 0.08 is introduced into the reaction vessel 11 from the second pump 8.
Inject ml. Then, the fluorescence intensity is measured under the same conditions as above. The fluorescence intensity measured previously and the fluorescence intensity measured this time are compared to determine the amount of methamphetamine contained in the sample liquid. Finally, the rotation of the stirring member 17 is stopped, the waste liquid valve 15 is opened, and the waste liquid pump 13 is driven to discard the waste liquid in the reaction container 11 into the waste liquid container 14. In order to clean the inside of the reaction vessel 11 and the piping in preparation for the next measurement, the third valve 6 and the switching valve 10 for the dye solution are switched, and the third pump 9 is driven to move the first valve therein. From storage container 3 of 3 to 6 × 10 ⁻⁷ M dye solution 1.
Aspirate 5 ml. After switching the third valve 6 and injecting 1.5 ml of the dye solution into the reaction container 11 from the third pump 9 to wash the inside of the reaction container 11, the waste liquid pump 1
3 and the like are activated to discard the waste liquid in the waste liquid container 14. By repeating this operation two to three times, the cleaning of the inside of the reaction container 11 and the piping is completed. Then, next, the sample liquid is exchanged with another one, and the above-mentioned respective steps are repeated. As a result, a characteristic curve showing the change in the concentration of methamphetamine in the sample liquid and the fluorescence intensity as shown in FIG. 4 is obtained. In the actual detection operation of methamphetamine or its derivative, the amount of methamphetamine in the sample liquid is determined in reverse from the fluorescence intensity of the sample liquid actually collected while referring to this characteristic curve. Here, for example, assuming that the fluorescence detector 12 can detect methamphetamine with a fluorescence intensity enhancement rate of 42% or less, the detection limit of the concentration of methamphetamine contained in the sample liquid is 1 × 10.
It turns out to be ^-7 M. Therefore, in this device, if the concentration of methamphetamine in the sample liquid is 1 × 10 ⁻⁷ M or more, its presence and amount can be determined.

[0018]

As described above, according to the present invention, the presence or absence of methamphetamine or its derivative in a sample or the amount thereof is not directly measured, but an antigen-antibody reaction using a fluorescent dye as a pseudoantigen is used, and a fluorescent dye is used. Since the presence or the content of methamphetamine or its derivative is indirectly detected by detecting the change in the fluorescence intensity of, the influence of impurities contained in the sample can be removed.

Since a predetermined amount of the sample liquid, the fluorescent dye and the anti-methamphetamine antibody are supplied to the reaction container from the storage container by the supplying means and the change in the fluorescence intensity of the fluorescent dye is detected, the methamphetamine or the methamphetamine in the sample liquid is detected. The derivative can be easily detected. Further, since the inlet and the air vent hole were provided in the upper part of the reaction vessel and the discharge nozzle was provided in the lower part, the methamphetamine in different samples could be continuously changed by controlling the opening and closing of the supply means and the discharge nozzle. Alternatively, it becomes possible to detect the presence or the amount of the derivative. Further, since the reaction container is provided with a stirring means driven by a magnetic force for stirring the sample solution, the fluorescent dye and the anti-methamphetamine antibody, the sample solution and the reagent can be easily stirred, and the process can be performed easily. The time required can be shortened. Furthermore, since the reaction vessel is provided with a thermostatic device using a Peltier element, the detection work can be performed under a constant temperature environment. Furthermore, since at least the storage container and the supply means are provided inside the air-conditioning type constant temperature device,
It becomes possible to detect methamphetamine and its derivatives under any temperature environment.

[Brief description of drawings]

FIG. 1 is a diagram showing a structural formula of a fluorescent dye having a methamphetamine structure.

FIG. 2 is a block diagram showing a configuration of a first embodiment of a detection apparatus for methamphetamine or a derivative thereof according to the present invention.

FIG. 3 is a perspective view showing the configuration of a reaction container in the first embodiment.

FIG. 4 is a graph showing a change in fluorescence intensity when the concentration of methamphetamine in a sample solution is changed by using the methamphetamine or its derivative detection device of the present invention.

FIG. 5 is a block diagram showing a configuration of a second embodiment of a detection apparatus for methamphetamine or a derivative thereof according to the present invention.

FIG. 6 is a block diagram showing the configuration of a third embodiment of the detection apparatus for methamphetamine or a derivative thereof according to the present invention.

7A is a front view showing the configuration of a thermostatic device according to a third embodiment, and FIG. 7B is a side view thereof.

FIG. 8 is a block diagram showing the configuration of a fourth embodiment of the detection apparatus for methamphetamine or a derivative thereof according to the present invention.

[Explanation of symbols]

 1, 2, 3: Storage container 4, 5, 6: Valve 7, 8, 9: Pump 10: Switching valve 11: Reaction container 12: Fluorescence detector 13: Discharge pump 14: Waste liquid container 15: Discharge valve 16 : Drive source 17: Stirring member 18: Constant temperature device 19: Air conditioning type constant temperature device 20: Constant temperature room 21: Computer 31: Block 32: Peltier element 33: Fin

Claims (6)

[Claims] 1. A sample solution prepared by dissolving a powder sample in a fluorescent dye solution having a methamphetamine structure is reacted with an anti-methamphetamine antibody, and the presence or absence of methamphetamine or a derivative thereof in the sample solution is determined from the change in fluorescence intensity of the sample solution. A detection device for measuring methamphetamine or a derivative thereof, wherein the sample solution, a fluorescent dye solution and an anti-methamphetamine antibody solution are respectively stored, and a reaction container for injecting and reacting the sample solution and the antibody solution, Fluorescence detecting means for detecting the fluorescence intensity of the solution to be measured in the reaction container, and a predetermined amount of the sample solution, the fluorescent dye solution and the anti-methamphetamine antibody solution are supplied from the respective storage containers to the reaction container, respectively. And a supply means for operating the methamphetamine. An apparatus for detecting min or its derivative. 2. The detection device for methamphetamine or a derivative thereof according to claim 1, wherein the supply means is composed of a syringe type pump and a valve. 3. The detector for methamphetamine or a derivative thereof according to claim 1, wherein the reaction container is a prismatic cell having an inlet and an air vent hole in the upper part and an exhaust nozzle in the lower part. 4. The detection device for methamphetamine or a derivative thereof according to claim 1, wherein the reaction container has therein stirring means driven by magnetic force for stirring the sample solution, the fluorescent dye and the anti-methamphetamine antibody. 5. The detection apparatus for methamphetamine or a derivative thereof according to claim 1, wherein the reaction vessel is provided with a thermostat using a Peltier element. 6. The detection apparatus for methamphetamine or a derivative thereof according to claim 1, wherein at least the storage container and the supply means are provided inside the air-conditioning type thermostatic device.