JPH06259533A - Optical image reconstituting device - Google Patents

Abstract

PURPOSE:To provide an optical image reconstituting device capable of reconsti tuting an optical tomographic image or an optical three-dimensional (3-D) image having excellent resolution or SN ratio and effective for practical use. CONSTITUTION:The optical image reconstituting device is provided with an optical image formation system 220, a focus plane control means 230 for moving the position of an object face focused by the system 220, an image pickup means 300 for converting an object image formed by the system 200 into an electric signal, an image input means 402 for preparing a segmented image obtained by stacking an image signal inputted by the means 300 in accordance with the position of a focus plane, a means for inputting images from plural angular directions on the same plane, a means 405 for positioning plural segmented images, and a means 405 for preparing a tomographic image by synthesizing plural segmented images.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a technique for reconstructing and displaying a tomographic image or a three-dimensional image by digital processing in an image input processing device using an optical instrument such as a microscope or a visualization device for various luminescence developments. .

[0002]

2. Description of the Related Art In a conventional image input processing apparatus using optical equipment, it may be difficult to input an image from an arbitrary direction due to structural restrictions. Consider a microscope as an example.
FIG. 11 shows a configuration diagram of a general microscope. Generally, a microscope is constructed so that an image of an object placed on a stage surface perpendicular to the optical axis direction of the optical system is formed. As shown in FIG. 11, in the direction perpendicular to the optical axis of the objective lens 103, that is, in the horizontal direction, an arbitrary portion of the object can be set within the visual field by the XY stage drive unit 101. The Z stage driving unit 102 in the direction is used for focus adjustment, and it is not possible to obtain a tomographic image parallel to the optical axis in the normal observation direction.

Examples of reconstructing a tomographic image parallel to the optical axis by a digital image processing method include A. Erhardt, G. Zins.
er, D.Komitowski and J.Bille, Appl.opt., 24,194-200,
(1985). In this paper, a three-dimensional image (hereinafter referred to as “intersection”) is obtained by inputting an image corresponding to a set focal plane while stepwise moving the position of a focused object plane (hereinafter referred to as “focus plane”) in the optical axis direction. Called a statue),
A method of recovering a spatial frequency component deteriorated at the time of image input by applying an inverse filter of a three-dimensional optical transfer function (3D-OTF) to this is shown.

However, in such a method, the spatial frequency characteristic in the optical axis direction is greatly deteriorated as compared with the surface direction due to the limitation of the numerical aperture (NA) of the microscope objective lens.
Even if recovery filtering is performed, it is difficult to obtain a fault plane with excellent resolution. Further, when the inverse filter is operated, the signal-noise ratio (S / N) of the reconstructed image may be extremely deteriorated by forcibly emphasizing the spatial frequency component whose 3D-OTF characteristic is significantly inferior. There is also.

[0005]

As described above, the conventional method in the field for obtaining an optical tomographic image is obtained within the limits of the optical characteristics such as the pass spatial frequency band and the depth of focus of the optical imaging system. Since the processing is performed using the obtained image information, there is a problem in terms of resolution and S / N.

The optical image reconstructing apparatus of the present invention has been made in view of such a problem, and its purpose is to obtain an optical tomographic image or an optical three-dimensional image excellent in resolution and S / N. An object of the present invention is to provide an optical image reconstructing device that can be reconstructed and is practically useful.

[0007]

In order to achieve the above-mentioned object, an optical imaging system, and a focusing surface control means for moving the position of a focused object surface in the optical imaging system, Image pickup means for converting an image of an object formed by the optical image forming system into an electric signal, and an image for forming a slice image in which image signals input by the image pickup means are stacked according to the position of the focusing surface. Input means, means for performing the image input from a plurality of angle directions within the same plane, means for aligning the plurality of section images, and means for creating a tomographic image by combining the plurality of section images. And.

[0008]

Embodiments of the present invention will be described below with reference to the drawings.

First, a first embodiment of the present invention will be described. In the present embodiment, by combining the slice images obtained in different optical axis directions, the spatial frequency characteristic in the optical axis direction, which was inferior to the slice images obtained in one direction, is improved, and a three-dimensional image with good characteristics is obtained. In addition, when performing synthesis, positional deviation correction is performed on the intercept images obtained in different directions, thereby reducing the load on the device configuration that requires spatial accuracy.

The slice image is obtained by stacking the input images while moving the focusing surface of the optical imaging system in the optical axis direction. A section image obtained for the object shown in FIG. 4A is shown in FIG. At this time, the spatial frequency characteristic of the intercept image given from the spatial frequency characteristic of the optical imaging system is shown in FIG.
It becomes like (c).

FIG. 4 (c) is a two-dimensional representation of the spatial frequency characteristic of the optical image-forming system in which the characteristic in the isotropic focusing plane direction is represented by an appropriate one direction and combined with the characteristic in the optical axis direction. Is. FIG. 4D shows a profile of the spatial frequency characteristic of FIG. 4C in the focusing plane direction. A profile in the optical axis direction is shown in FIG. It is understood from FIGS. 4D and 4E that the spatial frequency characteristic in the optical axis direction is significantly inferior to the spatial frequency characteristic in the focusing surface direction.

A method of correcting the positional deviation will be described below. Positioning of slice images obtained from different directions is performed using a correlation calculation. Since the intercept image is three-dimensional, the correlation calculation is as follows.

[0013]

[Equation 1] Here, c (x, y, z) is a correlation function, and * represents a complex conjugate. The correlation function c (x, y, z) can also be expressed as follows.

[0014]

[Equation 2] However, F [] represents the Fourier transform, and F [f (x,
y, z)] = F (u, v, w), F [g (x, y,
z)] = G (u, v, w). By obtaining the correlation function c (x, y, z) by the equation (2) and finding the peak position, the shift amount of the intercept image necessary for the position shift correction can be obtained. Position shift correction can be performed by shifting the shift amount of the intercept image and performing synthesis. According to the above-described positional deviation correction, the load of the apparatus configuration, which requires the spatial accuracy in this embodiment, can be reduced.

Next, the synthesis of slice images obtained in different optical axis directions will be described. The object shown in FIG. 5 (a),
Section image shown in FIG. 5B, FIG. 5C and FIG.
The composite image shown in (d) is a tomographic image obtained on a tomographic plane parallel to the rotation plane of the object shown in FIG.

The synthesis will be described below using a tomographic image. When it is desired to obtain a three-dimensional image from the tomographic images, the combined tomographic images may be stacked in a predetermined positional relationship. Figure 5 (a)
FIG. 5 (b) shows a section image obtained for the object. The intercept image of FIG. 5B is the sharpest image obtained when the focal plane exists at the position where the object exists, and the blur of the image increases as the focal plane moves away from the position where the object exists. . Further, FIG. 6A shows the spatial frequency characteristic of the intercept image.

FIG. 6A is a simplified view of FIG. 4C looking down from above. The shaded area is shown in Figure 4.
In the diagram of (c), it is a region having a height, that is, a region having a value other than zero. 5C and 5D show composite images obtained by obtaining such slice images from multiple directions and adding them. Figure 5
5C shows the case where the number of directions is 2, and FIG. 5D shows the case where the number of directions is 4. The spatial frequency characteristics of each composite image are shown in FIGS. 6 (b) and 6 (c). These spatial frequency characteristics show that by synthesizing intercept images obtained from multiple directions, it is possible to compensate for the band that is lost only by using intercept images in one direction, and a synthesized image with good characteristics can be obtained. ing.

The obtained composite image will be described below.
The combined image is obtained by removing the blurred image of the out-of-focus position generated when the image is input using the optical imaging system for the following reason. First, when a slice image is obtained, a blurred image that is out of focus and continuous in the optical axis direction disappears by addition and normalization of slice images in different directions except for the position where the object exists. Also, in the section image, the sharpest image should be at the position where the object exists, so the only image left by combining is the sharpest image. Therefore, by performing the combination, the blurred image which is caused by the optical image forming system and which is deviated from the in-focus position continuous in the optical axis direction is removed.

FIG. 1 is a view showing the arrangement of the optical image reconstructing apparatus according to the first embodiment. The optical image reconstruction device of FIG. 1 is roughly divided into a microscope device 200, a TV camera 300, an image processor 400, a TV monitor 500, and a man-machine interface 600. The microscope apparatus 200 is provided with a sample rotating device 210, and is configured so that it can be rotated while holding the sample. The objective lens 220 is configured to be driven in the optical axis direction by the focusing surface drive device 230, and the objective lens 22
The in-focus surface of 0 is set as a predetermined object plane in the optical axis direction.

FIG. 2 is a diagram showing the structure of the sample rotating device 210. The pulley 212 is rotationally controlled by the motor 211, and the power thereof is transmitted to the transparent tube 214 by the belt 213. The transparent tube 214 is filled with a liquid such as physiological saline or alcohol, and holds a sample such as living tissue or living cells. In addition, the transparent tube 214 is the holding table 21.
It is set so that it can rotate while being supported by 5a and 215b.

A configuration other than the microscope apparatus 200 in FIG. 1 will be described. The TV camera 300 is the microscope device 20.
It is attached to the tip of the lens barrel of 0 and the microscope is imaged. The image processor 400 includes an A / D converter 401 for digitally converting an analog video signal from the TV camera 300 and an image memory 402, and stores the digital image signal in accordance with the spatial positional relationship of the focusing surface at the time of image pickup. Is configured to be.

Further, an image memory 409 for recording a plurality of image signals, a CPU 405, an image memory 406, an FFT calculator 411, and a voxel processor 408 are connected to the internal bus 410, and based on a command from the CPU 405,
A two-dimensional image is reconstructed, and a predetermined two-dimensional image is calculated from the three-dimensional image by the voxel processor 408 and stored in the image memory 406. The image stored in the image memory 406 is converted into a predetermined analog video signal by the video D / A converter 407, and the TV monitor 500 is displayed.
Is displayed in.

Further, in the image processor 400, a sample rotator driver 404 for controlling the sample rotator 210.
A focusing surface driving device driver 403 for controlling the focusing surface driving device 230 is built in, and sends a control signal to each driving device based on a command signal from the CPU 405. The man-machine interface 600 is connected to the CPU 405,
The setting conditions and the like are displayed, and the operator can send operation commands and the like.

The operation of the above configuration will be described below.

In the image processor 400, an image obtained by picking up an object while moving the focusing surface of the objective lens 220 over the range completely included from the lowermost portion to the uppermost portion of the transparent tube 214 is the section image shown in FIG. As described above, according to the signal from the focusing surface driving device driver 403, it is stored in the image memory 402 according to the spatial positional relationship of the focusing surface. The completed slice image for one projection direction is sent from the image memory 402 to the image memory 409.

This operation is repeated until the set angle of the transparent tube 214 with respect to the optical axis of the objective lens 220 is changed by a predetermined interval until it makes one rotation, and the image memory 409 stores multidirectional slice images. The CPU 405 sets one appropriate intercept image as a reference and obtains a correlation function with another arbitrary intercept image using the FFT calculator 411. The CPU 405 detects the peak position of the correlation function to obtain the position shift amount of an arbitrary slice image with respect to the reference slice image. CPU
Reference numeral 405 synthesizes a three-dimensional image by adding the slice images from multiple directions stored in the image memory 409 while shifting them by the amount of positional deviation.

However, although the intercept image obtained in this embodiment is a three-dimensional image, the core synthesis is performed in two dimensions by advancing the processing as follows. CP
U405 cuts a three-dimensional slice image in the direction of the rotation surface of the rotation operation performed by the sample rotating device 210 to obtain a two-dimensional slice image. The CPU 405 obtains a two-dimensional composite image by performing addition processing including interpolation processing on the multi-directional two-dimensional intercept images while performing positional deviation correction. A three-dimensional image is obtained by stacking two-dimensional composite images. The combined three-dimensional image is stored in the image memory 409 again. Image memory 409
The voxel processor 408 executes a predetermined process so that the three-dimensional image stored in is visualized as an image displayed on the TV monitor 500. That is, by converting a three-dimensional image into a projection image viewed from a predetermined viewpoint, forming a predetermined cut plane image, or forming a predetermined isodensity plane, the three-dimensional image can be used according to the purpose. It is converted into an image that can be easily visually recognized and displayed.

As described above in detail, in the first embodiment, by combining the slice images obtained from different multiple directions,
The spatial frequency characteristic in the optical axis direction, which was inferior to the one-direction slice image, is improved. Further, when synthesizing the slice images, the positional deviation is corrected to reduce the load on the apparatus configuration that requires spatial accuracy.

Therefore, according to the first embodiment, it is possible to provide an optical image reconstructing apparatus which can reconstruct an optical tomographic image or an optical three-dimensional image having excellent resolution and which is practically useful.

The second embodiment of the present invention will be described below. The second embodiment is an example applicable to the case where the band limitation in the optical axis direction of the optical imaging system is inferior to that in the focusing surface direction, but is not so severe. The characteristics of the intercept obtained in this case are relatively inferior in the characteristics in the optical axis direction as compared with the in-focus surface direction, but are relatively good characteristics because the band limitation in the optical axis direction is not severe. Therefore, the synthesis of the intercept images in different projection directions to compensate for the band deterioration in the optical axis direction is
It can be performed even if the number of projection directions is reduced. In the second embodiment, in the reconstruction capable of reducing the number of projection directions, by preparing only the number of projection directions of the optical image forming system for inputting an image, a troublesome rotation operation such as adjustment is omitted and the operation is simplified. It is to measure

FIG. 7 is a view showing the arrangement of the optical image reconstructing apparatus according to the second embodiment. The configuration of FIG. 7 is roughly the microscope device 700, the TV camera 800, the TV camera 801,
It is divided into an image processor 900, a TV monitor 1000, and a man-machine interface 1100. A transparent tube 714 is fixed to the microscope apparatus 700 by a stand of a microscope apparatus (not shown). The transparent tube 714 is filled with a liquid such as physiological saline or alcohol, and holds a sample such as living tissue or living cells.

Two devices for inputting an image are prepared in the microscope device 700, and they are fixed so that their optical axis directions are orthogonal to each other in a plane orthogonal to the axis of the transparent tube. An objective lens 720 that constitutes a device for inputting an image is configured to be driven in the optical axis direction by a focusing surface driving device 730, and the focusing surface of the objective lens 720 is a predetermined object plane in the optical axis direction. Is set to. The objective lens 721 that constitutes the other device for inputting an image also
Similarly, the focusing surface driving device 731 is configured to be driven in the optical axis direction, and the focusing surface of the objective lens 721 is set to a predetermined object surface in the optical axis direction.

A configuration other than the microscope apparatus 700 in FIG. 7 will be described. The TV camera 800 is the microscope device 70.
It is attached to the tip of the lens barrel 740 inside 0 and captures the image obtained through the objective lens 720. The TV camera 801 is attached to the tip of the lens barrel 741 in the microscope apparatus 700 and captures an image obtained through the objective lens 721. In the image processor 900, an A / D converter 901 for converting an analog video signal from the TV camera 800 into a digital signal, an A / D converter 902 for converting an analog video signal from the TV camera 801 into a digital signal,
An image memory 903 is included and configured to store digital image signals in accordance with the spatial positional relationship of the focusing surface at the time of image pickup.

Further, a CPU 905, an image memory 906,
FFT calculator 910 and voxel processor 908
Is connected to the internal bus 909, a three-dimensional image is combined based on a command from the CPU 905, and a predetermined two-dimensional image is calculated from the three-dimensional image by the voxel processor 908 and stored in the image memory 906. Image memory 9
The image stored in 06 is converted into a predetermined analog video signal by the video D / A converter 907 and displayed on the TV monitor 1000.

Further, in the image processor 900, a focusing surface driving device driver 730 and a focusing surface driving device driver 904 for controlling the focusing surface driving device 731 are built in, and a CPU 905.
A control signal is sent based on the command signal from. The man-machine interface 1000 is connected to the CPU 905,
The setting conditions and the like are displayed, and the operator can send operation commands and the like.

The operation of the above configuration will be described below.

Within the image processor 900, the objective lens 72 over the entire included area of the transparent tube 714.
An image obtained by picking up an object while moving the focal plane of 0 is a focal plane driving device driver 90 as shown by a section image a in FIG.
It is stored in the image memory 903 according to the spatial positional relationship of the in-focus surface according to the signal of No. 4. Further, an image obtained by capturing an object while moving the focusing surface of the objective lens 721 over the range completely included from the lower part to the upper part of the transparent tube 714 is a focusing surface driving device as shown in a section image b in FIG. It is stored in the image memory 903 according to the spatial positional relationship of the focusing surface according to the signal of the driver 904.

The CPU 905 sets one intercept image as a reference, and obtains a correlation function with another intercept image using the FFT calculator 910. The CPU 905 detects the peak position of the correlation function to obtain the position shift amount of an arbitrary slice image with respect to the reference slice image. CPU 905 is an image memory 90
While shifting the slice image a and the slice image b stored in No. 3 by the positional displacement amount, addition is performed in the positional relationship shown in FIG. 8 to obtain a combined image.

The synthesized three-dimensional image is again used in the image memory 90.
3 is stored. The three-dimensional image stored in the image memory 903 is subjected to predetermined processing by the voxel processor 908 so that the three-dimensional image is visualized as an image displayed on the TV monitor 1000. That is, by converting a three-dimensional image into a projection image viewed from a predetermined viewpoint, forming a predetermined cut plane image, or forming a predetermined isodensity plane, the three-dimensional image can be used according to the purpose. It is converted into an image that can be easily visually recognized and displayed.

As described in detail above, in the second embodiment, when the image input is performed using the optical imaging system, if the band limitation in the optical axis direction is not so severe, the number of projection directions is reduced and the image input is performed. There are prepared as many optical imaging systems as the number of projection directions. By doing so, it is possible to omit complicated rotation operations such as adjustment in the reconstructing device. Further, the acquisition time of the section images can be shortened by simultaneously acquiring the section images from multiple directions.

The third embodiment of the present invention will be described below.
In the third embodiment, a case will be described in which the restoration filtering performed by utilizing the spatial frequency characteristic of the optical imaging system is applied to this embodiment. It is difficult to perform the restoration filtering using the spatial frequency characteristic of the optical imaging system on the intercept image obtained in a single projection direction. This is because the spatial frequency characteristic of the intercept image is severely band-limited in the projection direction due to the influence of the optical imaging system. In addition, there is a possibility that the S / N of the intercept image after the recovery processing may be extremely deteriorated by forcibly emphasizing the inferior spatial frequency component.

In the present embodiment, the intercept images in different projection directions are combined, and the obtained combined image is subjected to the restoration filtering utilizing the characteristics of the optical image forming system. By performing the restoration filtering after the synthesis process, it is possible to avoid the restoration filtering for the spatial frequency components that are severely band-limited, and thus eliminate the possibility that the S / N becomes worse.

A method of obtaining a filter used for recovery filtering in this embodiment will be described. The filter R (u, v) used for the restoration filtering is obtained as follows using the spatial frequency characteristic of the optical imaging system.
First, consider the spatial frequency characteristics given to the intercept image. Since the intercept image is an image obtained through the optical imaging system, the spatial frequency characteristic is the spatial frequency characteristic itself of the optical imaging system.

Since the composite image is the sum of the intercept images in different projection directions, the spatial frequency characteristic is the sum of the spatial frequency characteristics of the optical imaging system in accordance with the projection direction. The spatial frequency characteristic given to the composite image is I (u,
v), for example, as shown in FIG. 5C, the spatial frequency characteristic I (u,
v) becomes like FIG.6 (b). This is obtained by adding the spatial frequency characteristics of the intercept image shown in FIG. 4C, that is, the spatial frequency characteristics of the optical imaging system, in the directions orthogonal to each other.

When the number of projection directions is further increased, the spatial frequency characteristics of the composite image can be similarly obtained by adding the spatial frequency characteristics of the intercept image according to the increased projection directions. The spatial frequency characteristic assumed in the composite image after the restoration filtering is O (u, v), and O (u, v) is shown in FIG.
It shows in (a). In the present embodiment, considering the S / N, the spatial frequency characteristic of the composite image after the recovery fill retarding is the spatial frequency characteristic obtained by in-focus imaging. Figure 10 (b)
Represents the two-dimensional spatial frequency characteristic shown in FIG.
It is a profile cut by a plane represented by = 0. Using the spatial frequency characteristic I (u, v) of the composite image before the recovery filtering and the spatial frequency characteristic O (u, v) assumed for the composite image after the recovery filtering as described above, the filter R (u, v) is used. ) Is represented by the following formula.

[0046]

[Equation 3] FIG. 10C shows how the filter R (u, v) is obtained.

By using the filter R (u, v) obtained above with respect to the tomographic image after the synthesis processing, it is not necessary to perform unreasonable emphasis on inferior spatial frequency components, and thus S /
Good recovery filtering can be performed while avoiding the possibility of N being deteriorated.

FIG. 9 is a view showing the arrangement of the optical image reconstructing apparatus according to the third embodiment of the present invention. The configuration of FIG. 9 is roughly divided into a microscope device 200, a TV camera 300, an image processor 1200, a TV monitor 500, and a man-machine interface 600. Microscope device 200, T
The V camera 300, the TV monitor 500, and the man-machine interface 600 are the same as those in the first embodiment.

The image processor 1200 includes an A / D converter 1201 for digitally converting an analog video signal from the TV camera 300 and an image memory 1202, and a digital image according to the spatial positional relationship of the focusing surface at the time of image pickup. The signal is configured to be stored. Further, the image memory 1209 for recording a plurality of image signals, the CPU 12
05, an image memory 1206, a voxel processor 1208, an FFT calculator 1211, and a ROM 1212 that stores the filter shown in Expression (3).
0, the three-dimensional image is reconstructed based on a command from the CPU 1205, and the voxel processor 1
A predetermined two-dimensional image is calculated from the three-dimensional image by 208 and stored in the image memory 1206.

The image stored in the image memory 1206 is D
A / A converter 1207 converts the analog video signal into a predetermined analog video signal and displays it on the TV monitor 500. Further, in the image processor 1200, a sample rotating device driver 1204 for controlling the sample rotating device 210 and a focusing surface driving device driver 1203 for controlling the focusing surface driving device 230 are built in, and based on a command signal from the CPU 1205, Send a control signal to each drive. The man-machine interface 600 is connected to the CPU 1205, and is configured so that setting conditions and the like are displayed and the operator can send operation commands and the like.

The operation of the above configuration will be described below.

In the image processor 1200, an image obtained by picking up an object while moving the focusing surface of the objective lens 220 over the range completely included from the lowermost portion to the uppermost portion of the transparent tube 214 is adjusted so as to be a section image. It is stored in the image memory 1202 in accordance with the spatial positional relationship of the in-focus surface according to the signal from the in-focus surface driver driver 1203. The completed slice image for one projection direction is sent from the image memory 1202 to the image memory 1209. This operation is the objective lens 2
The setting angle of the transparent tube 214 with respect to the optical axis of 20 is changed at predetermined intervals and repeated until it makes one rotation, and multi-directional section images are stored in the image memory 1209.

The CPU 1205 determines an appropriate one intercept image as a reference, and obtains a correlation function with another arbitrary intercept image by using the FFT calculator 1211. The CPU 1205 detects the peak position of the correlation function to obtain the amount of positional deviation of an arbitrary slice image with respect to the reference slice image.

The CPU 1205 adds three-dimensional images stored in the image memory 1209 while shifting the slice images from various directions by the amount of positional deviation. CP
U1205 is a sample rotation device 21 based on the synthesized three-dimensional image.
A tomographic image is obtained in the direction of the plane of rotation of the rotation operation performed by 0.

The CPU 1205 performs FFT on the obtained tomographic image.
Fourier transform is performed using a computing unit (DSP) 1211 to obtain a spatial frequency representation of a tomographic image. The CPU 1205 performs recovery filtering by multiplying the spatial frequency expression of the tomographic image by the filter stored in the ROM 1212 for each component. The CPU 1205 is the FFT calculator 12
Inverse Fourier transform is performed using 11 to obtain a tomographic image after recovery filtering. A three-dimensional image after recovery filtering is obtained by stacking the tomographic images after recovery filtering at a predetermined position. The obtained three-dimensional image is stored in the image memory 1209 again. The voxel processor 1 allows the three-dimensional image stored in the image memory 1209 to be visualized as an image displayed on the TV monitor 500.
Predetermined processing is executed by 208.

That is, a three-dimensional image is obtained by converting the three-dimensional image into a projected image viewed from a predetermined viewpoint, forming a predetermined cut surface image, or forming a predetermined equal density surface. Is converted into an image that can be easily visually recognized and displayed.

As described in detail above, in the third embodiment, the restoration filtering is performed after the synthesis process of the intercept image, so that the restoration filtering for the spatial frequency component which is severely band-limited can be avoided. It is possible to eliminate the possibility that the S / N ratio of the composite image will deteriorate.

[0058]

As described in detail above, in the optical image reconstructing apparatus of the present invention, an optical tomographic image or an optical three-dimensional image excellent in resolution and S / N can be reconstructed, and the optical image is practically useful. An image reconstruction device can be provided.

[Brief description of drawings]

FIG. 1 is a diagram showing a configuration of an optical image reconstructing apparatus according to a first embodiment.

FIG. 2 is a diagram showing a configuration of a sample rotation device.

FIG. 3 is a diagram showing a tomographic image obtained on a tomographic plane parallel to the rotation plane of the object.

FIG. 4 is a diagram for explaining the outline of the first embodiment.

FIG. 5 is a diagram for explaining composition of slice images obtained in different optical axis directions.

FIG. 6 is a diagram for explaining composition of slice images obtained in different optical axis directions.

FIG. 7 is a diagram showing a configuration of an optical image reconstructing device according to a second embodiment.

FIG. 8 is a diagram for explaining the operation of the second embodiment.

FIG. 9 is a diagram showing a configuration of an optical image reconstructing device according to a third embodiment.

FIG. 10 is a diagram for explaining how to obtain a spatial frequency characteristic and a filter R (u, v).

FIG. 11 is a configuration diagram of a conventional general microscope.

[Explanation of symbols]

200 ... Microscope device, 210 ... Sample rotation device, 220 ...
Objective lens, 230 ... Focusing plane drive device, 400 ... Image processor, 401 ... A / D converter, 402, 406, 4
09 ... Image memory, 403 ... Focus plane driving device driver,
404 ... Sample rotation device driver, 405 ... CPU, 40
7 ... D / A converter, 408 ... Voxel processor, 41
0 ... Internal bus, 411 ... FFT calculator, 500 ... TV monitor, 600 ... Man = machine interface.

Claims (1)

[Claims] 1. An optical imaging system, a focusing surface control means for moving a position of a focused object surface in the optical imaging system, and an object image formed by the optical imaging system. Means for converting the image signal into a dynamic signal, an image input means for creating a slice image in which image signals input by the image pickup means are stacked according to the position of the focusing surface, and a plurality of angles for the image input in the same plane. An optical image reconstructing apparatus comprising: a unit for performing a direction, a unit for aligning the plurality of slice images, and a unit for creating a tomographic image by synthesizing the plurality of slice images. .