JPH06247876A - Medicine for improving alcoholic hepatopathy - Google Patents

Abstract

PURPOSE:To obtain a new medicine containing gelatin and soluble collagen as the active components, excellent in safety, capable of economical oral large- volume administration over a long period and effective for improvement of alcoholic hepatopathy. CONSTITUTION:Gelatin or soluble collagen derived from the skin or the bone of an animal is powdered and prepared into a suitable dosage form without any further treatment or heated for formation of its sol and subsequently cooled, e.g. so as to be gelled. Thereby, this treatment enables oral administration thereof. In addition, the dosage is preferably 50 to 500mg per kg body weight per day for an adult male in general.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an alcoholic liver injury relieving agent containing gelatin or soluble collagen as an active ingredient.

[0002]

2. Description of the Related Art The functions of the liver include detoxification, metabolism of carbohydrates, proteins or fats, bile production and secretion, hormone regulation, blood coagulation (prothrombin) production, and storage of various biological constituents. It is known that there are many actions such as a hepatocyte regeneration action. However, the function of the liver is alcohol, malnutrition, viruses, drugs,
It may be damaged by toxic substances and various other factors. And, although the number of patients suffering from such disorders, including the potential population, is extremely large, a completely effective treatment method has not yet been established. Various substances have been reported to be effective in reducing or treating liver diseases in order to improve this disorder. For example, Cami Grenard (JP
60-258115), 2-hydroxy-3- (3,4-dihydro-
6-hydroxy-2,5,7,8-tetramethyl-2H-benzopyran
-2-yl) α-hydroxycarboxylic acid having a chroman skeleton such as propionic acid (JP-A-61-204122), guanidinoethanesulfonic acid (JP-A-62-138426)
Substituted 1,3-dithietane derivatives such as 2- (1,3-dithietan-2-iridin) -5,5-dimethyl-1,3-cyclohexanedione (JP-A-62-158214) ), Saponins (JP-A-3-31296), 2,2'-dithiobisbenzimidazole (JP-A-4-208223), methylenedioxyaniline derivatives (JP-A-4-193828) and so on. However, although some of these compounds have plant components, they are chemical substances and are not always satisfactory in terms of safety and effects when administered for a long period of time. On the other hand, in recent years, alcohol intake has become a habit, and as a result, various disorders due to alcohol intake, particularly alcoholic liver damage, have become a problem.
The measures are desired.

[0003]

DISCLOSURE OF THE INVENTION The inventors of the present invention are used to prevent or reduce the symptoms of various disorders caused by alcohol intake, in particular, to prevent or cure the symptoms of alcoholic liver disorder. For many years, we searched for possible active ingredients. For this reason, various naturally occurring components were examined, and it was found that gelatin or soluble collagen has an action of reducing alcoholic liver injury, and the present invention was completed. That is, an object of the present invention is to provide an alcohol liver injury relieving agent that is highly safe and can be obtained in large quantities at low cost.

[0004]

[Means for Solving the Problems] As described above, the present inventors investigated the relationship between protein and alcoholic liver injury by conducting animal tests in order to obtain a component effective for the treatment of alcoholic liver injury. As a result, they have found that the simultaneous ingestion of gelatin or soluble collagen and alcohol has an action of reducing alcoholic liver damage, and completed the present invention. That is, the present invention relates to an alcoholic liver injury relieving agent containing gelatin or soluble collagen as an active ingredient.

Gelatin, which is an active ingredient of the present invention, is a kind of protein obtained by purifying glue from animal skins and bones, and is used in many foods. The protein that constitutes gelatin is one in which collagen, which is one of the important structural proteins in the living body, is solubilized and the collagen molecule is partially decomposed. A solubilized collagen molecule without being decomposed is called soluble collagen. Gelatin and soluble collagen are characterized by containing a large amount of glycine, arginine, proline and hydroxyproline, which are types of amino acids. Glycine is known to have an action of suppressing an increase in blood cholesterol concentration in rats fed with methionine in excess of the amino acid requirement [Essential Amino Acid Research, 102, 20-25 (1984)]. Ingested arginine is changed to alanine in vivo, and this alanine is known to be a representative gluconeogenic amino acid [Harper, "Biochemistry" (21th edition) 298, (1988)]. . However, it has not been found that gelatin or soluble collagen has an action of reducing alcoholic liver injury as in the present invention. Such gelatin and soluble collagen are
A commercially available product can be used. Commercially available gelatin includes "gelatin MJ" (manufactured by Nitta Gelatin Co., Ltd.), "gelatin S-1" (manufactured by Nippi Co., Ltd.), and soluble collagen is "collagen powder P".
K ”(manufactured by Nitta Gelatin Co., Ltd.) and the like.

These gelatin and soluble collagen may be ingested orally as a powder or in a suitable dosage form orally, or may be heated to form a sol, or may be further cooled to form a gel, and orally ingested. it can.
In addition, other dosage forms suitable for oral administration are administered to humans or animals in some cases.

[0007] Oral administration agents include tablets, capsules,
There are granules, fine granules, powders, etc., and usual pharmaceutical auxiliaries, for example, binders such as sorbit and polyvinylpyrrolidone,
Formulation is carried out by an ordinary formulation method together with excipients such as lactose and corn starch, lubricants such as magnesium stearate and talc, disintegrating agents such as potato starch and carboxymethyl cellulose, and wetting agents such as sodium laurate. The tablets may be coated if desired.

Since the active ingredient of the present invention is gelatin or soluble collagen, it has almost no acute toxicity. Therefore, it is used as it is, or various nutritional ingredients, seasoning ingredients,
It is also possible to add a flavor component, a coloring component, etc., and mold the mixture into an appropriate shape to use it as a food or drink as a functional food, a food for specified health use, or a health food. Nutritional components include various vitamins and minerals, seasoning components include sucrose, L-glutamic acid, inosinic acid, citric acid, etc., flavor components include various flavors, and color components include food colorants. Is used.

The dose of gelatin or soluble collagen as an active ingredient is not particularly limited as long as it is an effective amount or more, since these ingredients are extremely safe and inexpensive and can be supplied in a large amount. 50-500m per kg of body weight
g is appropriate. If it is 50 mg or less, there is no liver damage reducing action, and if it is 500 mg or more, the reducing action does not increase even if the dose is increased. The following animal tests were carried out to show the liver damage reducing action of the present invention.

Gelatin manufactured by Wako Pure Chemical Industries, Ltd. was used. Four-week-old male Wistar rats (CLEA Japan, Inc.) were preliminarily fed with a standard liquid feed containing no alcohol for 5 days, and then 1
Each group was divided into 2 groups of 8 animals and fed with the experimental feeds shown in Table 1 for 5 weeks. The ratio of gelatin to casein in the test feed was 3 to 7. Further, in order to prevent the test feed from being deficient in essential amino acids, supplements were made so that the content of each essential amino acid in the feed would reach 2/3 of the Miyazaki pattern. The γ-GTP level in serum is
Rats were laparotomized under anesthesia with sodium pentobarbital, blood was collected from the posterior vena cava, and serum was separated. The enzyme method (Hiroshi Maruyama et al., Latest test, 5 , 87 (1987), "Determiner manufactured by Kyowa Medex Co., Ltd." γ-GTP ”). Table 1 shows the component composition of the feed used in the animal experiments.
The γ-GTP level in serum after the administration of the experimental feed is shown in FIG. As shown in this figure, γ-GTP of the test group to which gelatin was administered
The value is significantly lower than the control group not administered,
From this, it was found that gelatin is effective in reducing the symptoms of alcoholic liver injury. In addition, soluble collagen (“Collagen Powder P” manufactured by Nitta Gelatin Co., Ltd.)
K ”) was used to carry out a method similar to the above, but almost the same results were obtained, and soluble collagen was also effective in alleviating alcoholic liver damage as was gelatin.

[0011]

[Table 1] Test feed composition (g / L) ───────────────────────────── Control feed Test feed ───── ──────────────────────── Corn oil 38.0000 38.0000 Corn starch 33.9000 33.9000 Vitamin mixture 0.0853 0.0853 Salt mixture 7.7177 7.7177 Choline tartrate 0.5300 0.5300 Ethyl alcohol (99.5% ) 50.7714 50.7714 Casein 47.6000 31.7300 Gelatin 0.0000 17.7500 Methionine 0.0000 0.2590 Cysteine 0.7640 0.9060 Phenylalanine 0.0000 0.2250 Tryptophan 0.0000 0.1220 Monoglyceride 4.0000 4.0000 ────────────────────────── Since gelatin was added to the test feed, essential amino acids methionine, phenylalanine, and tryptophan, which are lacking in gelatin, were added. Also, since cysteine derived from casein was reduced, cysteine was increased so that the cysteine content was equal to that of the control diet.

Next, the present invention will be specifically described with reference to examples.

[0013]

Example 1 250 g of lactose was added to 100 g of gelatin powder (manufactured by Wako Pure Chemical Industries, Ltd.) and mixed well, and potato starch was further added.
150 g and 3 g of magnesium stearate were added, and the mixture was compressed with a tableting machine to give tablets.

[0014]

Example 2 100 g of soluble collagen powder (“Collagen powder PK” manufactured by Nitta Gelatin Co., Ltd.) was added with 150 sucrose.
g and lactose (250 g) were added and pressed to obtain a stick-shaped solid substance.

[0015]

EFFECT OF THE INVENTION Oral administration of proteins derived from animal skins and bones such as gelatin and soluble collagen can effectively reduce alcoholic liver damage. In particular, since the active ingredients are gelatin and soluble collagen, they are extremely safe and can be administered in large quantities at low cost for a long period of time.

[Brief description of drawings]

FIG. 1 Serum γ-GTP of control group and test group in test examples
Values (mean ± standard error) are shown. In the figure, * indicates that there is a significant difference between the control group and the test group (p <0.05).

Claims (1)

[Claims] 1. An alcoholic hepatic disorder relieving agent comprising gelatin or soluble collagen as an active ingredient.