JPH06245759A - Microorganism having oil and fat productivity and production of oil and fat - Google Patents

Abstract

PURPOSE:To provide a microorganism having a high oil and fat productivity and capable of producing fats and oils containing palmitoleic acid as a constituting fatty acid in a high ratio, a method for production of fats and oils using the same microorganism and an effective method for recovering a fatty acid, a by-product formed in the fat and oil production process in the form of fat and oil. CONSTITUTION:There is provided Trichosporon species SH45Y-E228 strain (Bikoken stipulation no. 13406). This strain is cultured in a culture medium containing a fatty acid alkyl ester or a fatty acid so as to extracellularly produce and accumulate a fat and oil in the culture solution. The resultant accumulated fat and oil is collected from the culture solution.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism having a high oil and fat producing capacity. TECHNICAL FIELD The present invention relates to a method for producing fats and oils by high-yield production and accumulation in the extracellular culture solution by fermentation. The present invention also relates to a method for producing fats and oils containing a high proportion of palmitoleic acid as a constituent fatty acid. Furthermore, the present invention relates to a method for producing fats and oils from fatty acids, which are oil by-products.

[0002]

2. Description of the Related Art As a method for intracellular production of fats and oils by fermentation, Endomyce (Oidium) genus, Endomyce
s), Candida, Rhodotoru
la), Cryptococcus genus or Rhodosporidium genus microorganisms for producing cocoa butter substitute fat (Japanese Patent Laid-Open No. 51-12386)
8); Method for producing cocoa butter substitute fat by a microorganism belonging to the genus Endomyces, Rhodotorula or Lipomyces (Japanese Patent Laid-Open No. 52-122672); Saccharomyces (Sa
A method for producing a lipid component (including triglyceride) having a high palmitoleic acid content by a microorganism belonging to the genus ccharomyces (JP-A-63-287491); Mortierella
a) A method for producing lipids (including fats and oils) having a high γ-linolenic acid content by microorganisms belonging to the genus (JP-A-59-130191); and lipids having a high palmitoleic acid content due to microorganisms belonging to the genus Kloeckera A method for producing (including neutral lipid) is known (JP-A-1-108991), and production of oils and fats by microorganisms belonging to the genus Trychosporon has been reported [NJ Moon et al., J. Am. . Oil Che
m.Soc., 55 , 683-688 (1978) and H. Kaneko et al., Li
pids, 11 (No. 12), 837-844 (1976)]. It is also known to produce palmitoleic acid in Saccharomyces microorganisms (JP-A-62-289191).

As a method for producing lipids in vitro by fermentation, a method of culturing molds or algae in the presence of a surfactant is known (Japanese Patent Laid-Open No. 62-3791). In addition, Candida lipolytica (lypolytica), which has the ability to produce fatty acids in vitro
Has been reported on the extracellular production of fatty acids by mutants belonging to [T. Miyakawa et al., Agric. Biol. Chem., 48 ,
499 (1984)]. Palmitoleic acid is a type of monounsaturated fatty acid having 16 carbon atoms and is contained in mink oil and macadamian nut oil in an amount of 15 to 20%. Palmitoleic acid has a useful pharmacological action [antitumor action, tooth decay inhibitory action, and protective action against vascular disorders in hypertensive diseases (JP-A-62-289191), etc.]
In addition, oils and fats containing palmitoleic acid as a constituent component have good affinity with the skin and are useful as cosmetic materials. Cocoa butter contains a triglyceride mainly having a saturated fatty acid group at the α and α ′ positions and a oleic acid group which is a monoene fatty acid group mainly at the β position.

[0004]

In the case of the intracellular production of fats and oils, the extraction process of fats and oils is complicated. That is, first, the bacterial cells are recovered by centrifugation or filtration, and then the wet bacterial cells are crushed (mechanical crushing with a colloid mill, ball mill, homogenizer, etc., crushing with ultrasonic waves), and oils and fats are extracted with n-hexane etc. Or dry the wet cells (freeze drying,
Spray drying etc.), chloroform: methanol mixed solvent or n-
A step of extracting fats and oils with a hexane: isopropanol mixed solvent or the like is required. In addition, in the production of fats and oils by fungi, generally, the cultured bacterial cells are apt to agglomerate, so that the bacterial cells tend to be entangled with the stirring blade, destroyed by the stirring blades, and the reproducibility is not sufficient. In addition, in the production of fats and oils by algae, the culture time is generally 1 week to 1
It takes as long as a month and requires light irradiation equipment as an incidental equipment.
Further, in the extracellular production of fats and oils in the presence of a surfactant, after the culture is completed, generally, the produced fats and oils are extracted with an organic solvent. The problem of insufficient separation is likely to occur. An object of the present invention is to provide a microorganism having a high oil and fat production capacity. Another object of the present invention is to provide a microorganism having the ability to produce an oil or fat containing a high proportion of palmitoleic acid as a constituent fatty acid. Another object of the present invention is to provide a method for fermentatively producing fats and oils outside of bacterial cells in high yield. Another object of the present invention is to provide a method for fermentatively producing fats and oils containing palmitoleic acid as a constituent fatty acid in a high proportion. Another object of the present invention is to provide a method for facilitating the production of fats and oils outside the cells using yeast to facilitate the extraction step and reduce the cost. Another object of the present invention is to provide an extracellular fermentation production method of fats and oils which can avoid the problems of fats and oils production by molds and algae. Another object of the present invention is to provide an extracellular fermentation production method of fats and oils, which is advantageous in that extraction of fats and oils after fermentation production with an organic solvent facilitates separation of an aqueous phase and an organic solvent phase. Especially. A further object of the present invention is to provide a means for recovering, as fats and oils, fatty acids contained in distillate oil, soda oil syrup, etc., which are by-produced in the fats and oils manufacturing process, and which have not been effectively used in the past.

[0005]

[Means for Solving the Problems] Researchers including one of the inventors of the present invention have conducted research aiming at extracellular fermentation production of fats and oils, and as a result, use a normal fermentation medium such as glucose as a main carbon source. No extracellular fat production in culture in fermentation medium, Trichosporon, Saccharomycopsis, Candida or Cryptococcus oil-producing microorganisms belonging to the genus, by culturing in the presence of a fatty acid alkyl ester or fatty acid, It was found that fats and oils were produced and accumulated in a large amount outside the bacterial cell (Japanese Patent Application No. 3-272003). The present inventors mutated the microorganisms used in the above invention to obtain microorganisms having further excellent properties, and produced fats and oils, and the ability to produce fats and oils containing palmitoleic acid as a constituent fatty acid in a high ratio. It was possible to obtain a microorganism having a markedly increased amount, and the present invention was reached. That is,
The present invention is Trichosporon species SH45Y-E.
Regarding the 228 strain (Microtech Lab. No. 13406), the Trichosporon species SH45Y-E228 strain is further cultured in a medium containing a fatty acid alkyl ester or a fatty acid to generate and accumulate fats and oils outside the bacterial cells in the culture solution,
The present invention relates to a method for producing fats and oils, which comprises collecting fats and oils produced and accumulated from the culture solution.

The microorganism Trichosporone
Species SH45Y-E228 strain is the above-mentioned Japanese Patent Application No. 2-
The mutant strain obtained by mutating the Trichosporon species SH45Y used in the invention of 272003, which has the same mycological properties as the SH45Y strain, but has the fat and oil-producing ability and palmitoleic acid as a constituent fatty acid as described above. They differ in that their ability to produce high proportions of fats and oils is significantly increased. The mycological properties of the SH45Y strain are described in Japanese Patent Application No. 2-272003. The Trichosporon species SH45Y-E228 strain has been deposited with the Institute of Microbial Science and Technology of the Agency of Industrial Science and Technology, as mentioned above, as Micromachine Research Institute No. 13406.

Next, the fatty acid alkyl ester used in the present invention will be described. The acid component constituting the fatty acid alkyl ester is usually a saturated or unsaturated fatty acid having 8 to 22 carbon atoms, particularly 12 to 18 carbon atoms, such as caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid. , Linoleic acid, linolenic acid, arachidic acid, arachidonic acid, eicosapentaenoic acid, behenic acid,
Erucic acid may be mentioned. As the alcohol component of the fatty acid alkyl ester, lower alcohols having 1 to 6 carbon atoms, such as methanol, ethanol, n-propanol, i-propanol, n-butanol, etc. are usually mentioned, but ethanol is preferable. The fatty acid alkyl ester may be used alone or in combination of two or more kinds. Next, as the fatty acid used in the present invention, an unsaturated fatty acid having usually 8 to 22 carbon atoms, particularly 16 to 22 carbon atoms (for example, palmitoleic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, eicosapentaenoic acid, erucic acid), Saturated fatty acids having 8 to 16 carbon atoms (eg caprylic acid, capric acid,
Lauric acid, myristic acid, palmitic acid), or the above unsaturated fatty acid or saturated fatty acid and 18 to 22 carbon atoms,
In particular, a mixture with 18 to 20 saturated fatty acids (eg stearic acid, arachidic acid, behenic acid) can be mentioned. The unsaturated fatty acid or C ₈ -C ₁₆ saturated fatty acid in the mixture of C ₈ -C ₁₆ saturated fatty acid and C ₁₈ -C ₂₂ saturated fatty acid or C ₈ -C ₁₆
The proportion of saturated fatty acids is 10% by weight or more, preferably 25% by weight
That is all. In addition, as the mixture, vegetable oil / fat fatty acids, such as soybean fatty acid and rapeseed fatty acid, which are contained in distillate oil, soda oil syrup and the like produced as a by-product in the vegetable oil production process and have not been effectively used, can be used. In addition,
When a mixture of unsaturated fatty acid and C _{18 to} C ₂₂ saturated fatty acid is used as described above, not only unsaturated fatty acid but also C ₁₈
-C ₂₂ saturated fatty acids is also being assimilated by the present invention use yeast, for example, in the case of using stearic acid alone, which is hardly assimilated. Further, a fatty acid and a fatty acid alkyl ester may be used in combination, but when the above C _{18 to} C ₂₂ saturated fatty acid is used as the fatty acid, the proportion thereof is less than 90% by weight, preferably less than 75% by weight.

In culturing the yeast used in the present invention,
The amount of the fatty acid alkyl ester or fatty acid contained in the medium is generally 0.1 to 50 g / dl, preferably 1
~ 10g / dl. The fatty acid alkyl ester or fatty acid may be added from the beginning of the culture, may be added during the culture in which the bacteria have considerably grown, or may be a complex addition type thereof. That is, it is not necessary to maintain the above concentration over the entire period of culture, and specific control of the concentration may be appropriately determined in relation to the extracellular accumulation of oil and fat and its amount. Thus, by culturing in the above concentration range, fats and oils are produced and accumulated outside the cells. Further, as will be described later, the constituent fatty acid composition of the oil or fat changes depending on the type of fatty acid of the fatty acid alkyl ester to be added or the type of fatty acid to be added.

Regarding the medium used in the method of the present invention,
The medium usually used for fermentative production of fats and oils is used. That is, as shown in the examples, both a synthetic medium and a natural medium can be used as long as the medium contains a nitrogen source, an inorganic substance and other nutrients in addition to the main carbon source. As the carbon source, the above-mentioned fatty acid alkyl ester or fatty acid may be used alone, and a small amount, for example, 20% by weight or less, preferably 2% by weight or less, of another carbon source is added and used. May be. Such other carbon sources include glucose, sucrose, fructose, starch,
Various carbohydrates such as starch hydrolysates and molasses, alcohols such as ethanol, methanol, glycerol, polyalcohols, amino acids such as glutamic acid and aspartic acid, and hydrocarbons such as n-paraffin, etc., depending on the assimilability of the bacteria used. Can be used.

As the nitrogen source, inorganic organic nitrogen compounds such as ammonia, ammonium chloride, ammonium sulfate, ammonium carbonate, ammonium acetate, sodium nitrate and urea can be used. Further, as the nitrogen source, nitrogen-containing natural products such as peptone, meat extract, yeast extract, corn steve liquor, casein hydrolyzate, fish meal or its digest, defatted soybean meal or its digest can also be used. Inorganic substances include monopotassium phosphate, dipotassium phosphate, magnesium sulfate, sodium chloride, ferrous sulfate, ferrous chloride, manganese sulfate, calcium chloride, calcium carbonate, zinc sulfate, copper sulfate, boric acid / molybdic acid. Ammonium, potassium iodide, etc. can be used. If the microorganism used requires a specific nutrient (for example, vitamins such as biotin and thiamine) for growth, the nutrient must be added to the medium in an appropriate amount. When these nutrients are added by being included in the nitrogen-containing natural product used as the nitrogen source, it is not necessary to add the nutrients separately.

The culture is carried out under aerobic conditions such as shaking culture or deep agitation culture. The culture temperature is generally 20 to 35 ° C.
Is preferable, but other temperature conditions may be used as long as the temperature is such that the bacterium grows. The pH of the medium during culturing is 4.5 to 6.0 when fatty acid ester is used and 4.5 to 6.0 when fatty acid is used.
Maintaining at 7.2 is desirable for high yields. Usually, 3 to 7 days after the start of culture, a considerable amount of oil and fat is produced and accumulated outside the cells.

After completion of the culturing, an extraction solvent is added to the culture medium to extract oils and fats in the extraction solvent. Since part of the oil and fat is attached to the surface of the bacterial cells, the extraction solvent is usually added to the culture-finished liquid itself containing the bacterial cells or a partial concentrate thereof. In addition to the above extraction, the fats and oils remaining in the cells may be recovered by a conventional method. As an extraction solvent, an organic solvent that dissolves fats and oils and is liquid with no or poor miscibility with water at room temperature, such as a halogenated lower alkane, such as chloroform, methylene chloride, carbon tetrachloride, 1,2-dichloroethane;
N-hexane, ethyl ether; ethyl acetate; aromatic hydrocarbons such as benzene, toluene and xylene are preferably used. The amount of the extraction solvent added is not particularly limited as long as it can sufficiently recover the oils and fats produced and accumulated in the culture medium, but is generally 50 ml or more, preferably 100 ml to 3 L, and particularly preferably 1 ml of the culture-finished liquid. 100 ml to 1
It is L.

The fact that the oil / fat extracted by the extraction solvent is an oil / fat existing outside the bacterial cells means that the phospholipids constituting the cell membrane, which are also extracted when the intracellular oil / fat is extracted, are extracted. It does not substantially exist in the liquid (in contrast, for example, in Example 1 of the above-mentioned JP-A-63-287491, lipids are extracted from the dried bacterial cells with a chloroform-methanol mixture without destroying the bacterial cells. Not only simple lipids such as, but also complex lipids such as phospholipids are extracted together.) Intact cells are observed after the extraction procedure. It is clear from the fact that fats and oils cannot be extracted by such a simple operation (for example, refer to the prior art documents relating to fats and oils production described in the section of the prior art).

The oil or fat extracted in the extraction solvent can be directly used as a product by removing the extraction solvent by evaporation according to the purpose, or can be used as a raw material fatty acid ester or its decomposition product by column chromatography, distillation, deoxidation or the like. It can also be purified by removing contaminating components such as certain fatty acids or raw material fatty acids (removing fatty acid esters and fatty acids results in almost pure triglycerides), and if necessary, subjecting to treatments such as decolorization and deodorization. You can also These individual treatment operations are conventional methods for producing fats and oils (for example, Takaaki Miyakawa, “Actual Production of Edible Oil”, Extraction and purification from plants such as soybean, corn, and rapeseed, Koshobo (1988), Fermentation). It can be carried out in accordance with the above-mentioned JP-A-63-28491, JP-A-52-122672, etc.) and the general method for refining a fermented product for refining a produced oil and fat.

Cocoa butter is mainly composed of triglycerides having a stearic acid residue or a palmitic acid residue in the α and α'positions and an oleic acid residue in the β position. In the present invention, a long-chain saturated fatty acid (C: 16 to 20) or an ester thereof or a mixture thereof is used alone or a long-chain monounsaturated fatty acid saturated fatty acid (C: 16 to 20) or a raw material fatty acid alkyl or fatty acid. When used in combination with its ester or mixtures thereof, it generally has a composition similar to cocoa butter, that is, it has mainly long-chain saturated fatty acid residues (C: 16-20) in the α and α'positions. A fat or oil mainly composed of triglycerides having a long-chain monounsaturated fatty acid residue (C: 16 to 20) at the β-position can be obtained. However, when the long-chain monounsaturated fatty acid, its ester, or a mixture thereof is used in combination as described above, the proportion thereof should be 40% by weight or less in total. Also, when using a long-chain saturated fatty acid, regardless of the above, it shall be used in combination with the above other components,
The long-chain saturated fatty acid content is less than 90% by weight, preferably
It should be less than 75% by weight.

The composition of oils and fats produced and accumulated is greatly affected by the type of fatty acid alkyl ester or fatty acid added to the medium. That is, when using saturated fatty acid alkyl or saturated fatty acid as a carbon source, generally,
The proportion of the corresponding saturated fatty acid residue, or the corresponding saturated fatty acid residue and the corresponding unsaturated fatty acid (mainly monounsaturated fatty acid) residue in the resulting fat or oil increases. When unsaturated fatty acid alkyl or unsaturated fatty acid is used as the carbon source, the proportion of the corresponding unsaturated fatty acid residue in the resulting fat or oil is generally increased. When a mixture of unsaturated fatty acid and saturated fatty acid is used as a carbon source, generally,
The proportions of the corresponding unsaturated fatty acid residue and the corresponding saturated fatty acid residue in the resulting oil and fat increase.

[0017]

EXAMPLES Next, the method of the present invention will be specifically described by way of examples. Example 1 100 ml of the following medium (containing 1.0 g of ethyl palmitate as shown below) was added to Trichosporon species SH45Y-E228 strain (Microtechnology Research Institute, Inc. 13406).
The No.) was 10 ⁷ inoculum was shaken for 4 days of culture at 28 ° C.. After completion of the culturing, 15 ml of chloroform was added to the culture broth and extraction was performed 3 times. The chloroform extracts were combined to a fixed volume of 50 ml. To 2 ml of the chloroform extract, 1.0 mg of triheptadecanoin as an internal standard substance was added, concentrated, and then applied to TLC. n-hexane: diethyl ether: acetic acid (80:20:
It was developed in 1) and the triglyceride fraction was collected. To the triglyceride fraction, add 0.6 ml of 10% KOH methanol solution,
Heated at 80 ° C for 20 minutes. After cooling, 0.7 ml of boron trifluoride-methanol complex methanol solution was added and heated for 2.5 minutes. After cooling, 1.0 ml of n-hexane was added and further heated for 1.5 minutes. After cooling again, a large amount of saturated sodium chloride aqueous solution was added to collect the upper hexane layer. The hexane layer was dehydrated with anhydrous sodium sulfate, and then fatty acids were quantified by gas chromatography. The total fatty acid amount was multiplied by 1.05 to obtain the triglyceride amount. The results are shown in Table 1.

Medium NaNO ₃ 2.0 g, (NH ₄ ) SO ₄ 2.0 g, K ₂ HPO ₄ 1.0 g, KH ₂ PO _{4
7.0g, MgSO 4 · 7H 2 O0.3g} , CaCl 2 · 2H 2 O 0.1g, NaCl
0.5g, ethyl palmitate 10.0g, vitamin mixed aqueous solution
^{* 1 1} ml, mineral mixed aqueous solution ^{* 2} 1 ml, 1% chloramphenicol / ethanol 1 ml, distilled water to 1000 ml. ^{* 1. 2} Vitamin mixed solution shown below Biotin 2mg, Calcium pantothenate 400mg, Folic acid 2mg, Inositol 2000mg, Nicotinic acid 400m
g, n-aminobenzoic acid 200mg, pyridoxine hydrochloride
400mg, Riboflavin 200mg, Thiamine hydrochloride 400m
g, make up to 1000 ml with distilled water. Mineral aqueous mixture _{M n SO 4 · 4~5H 2 O} 60mg, Z n SO 4 · 7H 2 O, C u SO 4
・ 5H ₂ O 40mg, FeCl ₂・ 6H ₂ O 250mg, H ₃ BO ₃ 60mg,
_{(NH 4) 6 Mo 7 O} 24 · 4H 2 0 25mg, KI 100mg, distilled water 100
Make it 0 ml.

Comparative Example 1 The strain used was Trichosporon species SH45Y.
(Microtech Lab. No. 12362) or Trichosporon
Species SH45Y-E2
No. 4) (all of them are the microorganisms disclosed in Japanese Patent Application No. 3-272003) and were operated in the same manner as in Example 1. The results are shown in Table 1.

[Table 1] ──────────────────────────────────── Bacterial strain Triglyceride Fatty acid composition of triglyceride Ceride (wt %) (Mg) C ₁₆ C _{16: 1} C ₁₈ C _{18: 1} C _{18: 2} ────────────────────────────── ─────── (Example) Trichosporon species 153 59.1 35.2 0.5 3.1 2.1 SH45Y-E228 (Comparative example) Trichosporon species 113 63.0 15.9 1.4 16.4 3.3 SH45Y (Ministry of Microbiology Research Institute No. 12362) Trichosporon species 130 62.7 26.7 0.6 6.7 3.2 SH45Y-E2 (Microbiology Research Institute No. 12364) ──────────────────────────────── ───── C ₁₆ palmitic acid, C _{16: 1} palmitoleic acid, C
₁₈ Stearic acid, C _{18: 1} oleic acid, C _{18: 2} Linoleic acid

Example 2 The same medium as in Example 1 except that the content of ethyl palmitate was different in 5 L of jar fermenter (manufactured by Mitsuwa Rika) 3 L
(Including 60 g of ethyl palmitate) was added and sterilized. Trichosporon species SH45Y-E228 strain was inoculated into 200 ml of the same medium (containing 4.0 g of glucose) as in Example 1 except that glucose was used instead of ethyl palmitate, and the whole amount of the culture solution was cultured at 28 ° C. for 4 days. Inoculate the medium in a jar fermenter, 28 ℃, 250rpm, aeration
The cells were cultured under the condition of 0.5 L / min for 7 days. After completion of the culture, 500 ml of n-hexane (food grade) was added to the culture solution for extraction. The extraction operation was repeated 3 times in total, and the obtained extracts were combined and concentrated. The recovered oil was distilled to purify triglyceride. Using KDL1 (manufactured by LEYBOLD) 0.1mmHg, 140 ℃
The operation was repeated under the conditions described in 1. above and the distillation residue was purified by thin layer chromatography until a single spot of triglyceride was obtained. 16.2 g of triglyceride was obtained. The results are shown in Table 2.

[Table 2] ──────────────────────────────────── Triglyceride production Fatty acid composition of triglycerides (wt% ) (G) C₁₆ C_{16: 1} C₁₈ C_{18: 1} C_{18: 2}  ──────────────────────────────────── 16.2 60.3 30.4 0.6 5.0 3.7 ───────── ────────────────────────────

Example 3 Raw material 1 shown in the following table in place of 1 g / dl of ethyl palmitate
The same operation as in Example 1 was performed using the same medium as in Example 1 except that g / dl was contained. The results are shown in Table 3.

[Table 3] ──────────────────────────────────── Triglyceride Triglyceride fatty acid composition (wt%) Raw material Ceride ─────────────────────── Production (mg) C ₁₂ C ₁₄ C ₁₆ C _{16: 1} C ₁₈ C _{18: 1} C _{18: 2} ─ ─────────────────────────────────── Fatty acid myristic acid 164.3 0.2 85.0 1.7 − 0.3 9.4 3.4 Palmitic acid 15.4 − − 68.8 22.7 − 4.0 4.5 Palmitoleic acid 23.3 − − 3.4 94.0 − 2.6 − Oleic acid 125.6 − − − − − 95.5 4.5 Ethyl fatty acid ethyl ester Laurate 128.9 30.7 − 8.3 − 13.5 41.1 6.4 Ethyl myristate 123.3 − 71.5 3.9 − − 20.3 4.3 Ethyl oleate 21.3 − − 4.7 − − 89.8 5.5 ──────────────────────────────────── C ₁₂ : Lauric acid, C ₁₄ : Myristic acid

[0022]

INDUSTRIAL APPLICABILITY The present invention provides a microorganism having a high oil and fat producing ability and an ability to produce an oil and fat containing a high proportion of palmitoleic acid as a constituent fatty acid. Further, according to the method of the present invention, fats and oils can be fermented and produced from the precursor outside the cells at high yield. Further, according to the method of the present invention, fats and oils having a high content of palmitoleic acid as constituent fatty acids can be fermented and produced outside the cells from the precursor. Furthermore, according to the method of the present invention, the fatty acid by-produced in the oil / fat production step can be recovered as an oil / fat.

Continuation of front page (51) Int.Cl. ⁵ Identification code Office reference number FI technical display area C12R 1: 645)

Claims (3)

[Claims] 1. Trichosporon species SH45
Y-E228 strain (Ministry of Microbiology Research No. 13406). 2. Trichosporon. Species SH45
Y-E228 strain (Microtechnology Research Institute No. 13406) is cultured in a medium containing a fatty acid alkyl ester or a fatty acid to allow the fat and oil to be produced and accumulated outside the cells in the culture medium, and the fat and oil produced and accumulated from the culture medium. A method for producing fats and oils, which comprises collecting 3. The method for producing an oil or fat according to claim 2, wherein the oil or fat produced contains palmitoleic acid as a constituent fatty acid.