JPH06245753A - Apparatus for preventing contamination of culture box - Google Patents

Abstract

PURPOSE:To prevent the contamination of a culture chamber by placing a high-performance filter in a path for passing a gas to be circulated through the culture chamber. CONSTITUTION:The culture box 1 is provided with a main body 5 having a culture chamber 7 holding a specimen such as cell and maintained at a prescribed temperature, a feeding apparatus to feed a gas such as carbon dioxide gas to the culture chamber 7 of the main body and keep the concentration of the gas at a prescribed level, a path 13 connecting the upper part and the lower part of the culture chamber 7 and a blower 16 placed in the path to circulate the gas through the culture chamber 7. A high-performance filter 17 is placed in the path 13.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus for preventing contamination of culture vessels.

[0002]

2. Description of the Related Art In a conventional culture chamber, a supply device for supplying a gas such as carbon dioxide gas from the outside is provided in the culture chamber, and the gas in the culture chamber is circulated through this supply device to clean the gas supplied again in the culture chamber. A clean filter is provided so that clean gas is supplied into the culture chamber (see, for example, JP-A-60-141279).

[0003]

However, since the conventional carbon dioxide gas supplying device is provided with a filter in this supplying device, when the inside of the culture chamber is contaminated, the total amount of the culture medium inside the culture chamber is reduced by the filter. However, there was a problem that the gas in the culture could not be cleaned and the inside of the culture room had to be sterilized.

The present invention solves the above problems,
An object of the present invention is to provide a pollution control device for a culture cabinet, in which all the gas circulating in the culture cabinet is passed through a filter provided in the passage to clean the contaminated gas even if contamination occurs in the culture cabinet. It is a thing.

[0005]

The present invention relates to a main body having a culture chamber for accommodating a sample such as cells therein, a supply device for supplying a gas such as carbon dioxide gas to the culture chamber of the main body, and In a culture cabinet equipped with a passage that connects the upper part and the lower part to each other and a blower arranged in this passage to circulate the gas in the culture chamber, a filter is arranged in the passage.

[0006]

The present invention is constructed as described above,
This gas is filtered by a filter provided in a gas passage that circulates in the culture cabinet, and even if contamination occurs in the culture cabinet, the contaminated gas in the culture cabinet can be cleaned by the filter.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below with reference to the drawings.

FIG. 1 is a perspective view of a culture cabinet showing an embodiment of the present invention. FIG. 2 is a sectional view of the culture cabinet of the present invention. FIG. 3 is an exploded perspective view showing a state in which a filter is attached to the duct forming the circulation passage of the present invention.

Reference numeral 1 denotes an incubator comprising a main body 5 in which a heat insulating material 4 is filled between an outer case 2 and an inner case 3 made of stainless steel, and a door 6 for opening and closing the opening of the main body. . Inside the inner box 3 of the main body 5, a culture chamber 7 for culturing a sample such as cells while maintaining a predetermined temperature is formed. A water jacket (not shown) for heating the culture chamber 7 is provided outside the inner box 3. Reference numeral 8 is a supply device of carbon dioxide gas, nitrogen, etc. for maintaining the inside of the culture chamber 7 at a predetermined gas concentration.

The door 6 for closing the opening of the main body 5 is a heat-insulating outer door 9
And the inner door 10 inside thereof. And
Inside the inner door 10, a plurality of small inner doors 1 for closing the culture chamber 7
1 is openably and closably provided.

Reference numeral 12 is a duct mounted inside the inner box 3, and this duct has a passage 13 formed therein. The duct 12 has a suction hole 14 for sucking gas in the culture chamber 7.
Is provided on the upper side of the culture chamber, and a discharge hole 15 for discharging gas to the culture chamber 7 is provided on the lower side of the culture chamber.

A blower 16 is arranged in the passage 13 and circulates the gas in the culture chamber 7. A high performance filter 17 is arranged on the leeward side of the blower. This filter is formed into a bag by bending a flat filter.

In the apparatus for preventing contamination of an incubator constructed in this way, the incubator 1 is a water jacket and the incubator 7
The inside of the culture chamber 7 is maintained at a predetermined temperature while being heated to a predetermined temperature, and a gas such as carbon dioxide or nitrogen is supplied by a supply device 8 to maintain the inside of the culture chamber 7 at a predetermined concentration. The inside of the culture chamber 7 is set to the optimum conditions for culturing cells and the like.

The blower 16 has a suction hole 14 in the duct 12.
The gas in the culture chamber 7 is sucked in from the above and is blown from the lower side through the passage 13 to circulate the entire gas in the culture chamber 7 so that the temperature and the gas concentration in the culture chamber 7 become uniform. There is.

The gas circulating in the culture chamber 7 is forcibly sucked into the passage 13 formed by the duct 12 by the blower 16 so that it is filtered by the high-performance filter 17 arranged in this passage to obtain a clean gas. Has been

The high-performance filter 17 is formed in a bag shape and is arranged in the passage 13 of the duct 12 so that the blower 16
Therefore, all the gas ventilated in the passage 13 can be passed.

Pollutants flow into the culture chamber 7 from a supply device 8 for supplying a gas such as carbon dioxide or nitrogen, or the culture chamber 7
When the sample in the culture chamber 7 leaks and contamination occurs in the culture chamber 7, the gas in the culture chamber 7 is blown by the blower 16 into the duct 1.
2 is forcibly sucked into the passage 13 through the suction hole 14 and is filtered by the high-performance filter 17 to remove contaminants, and the gas passing through this filter is cleaned to clean the culture chamber 7.
The pollutants inside are removed. Therefore, even if the culture chamber 7 is contaminated, by operating the blower 16
The contamination in the inside can be eliminated, and the sterilization work of the culture room 7 can be made unnecessary when the contamination occurs.

Further, the high-performance filter 17 is the duct 12
Since the duct 12 is set and attached to the culture chamber 7, the duct 12 can be replaced by simply removing the duct 12.

According to the present invention, by disposing a high performance filter 17 in the passage 13 in which the gas circulates in the culture chamber 7,
Even if contamination occurs in the culture chamber 7, the contamination in the culture chamber 7 can be eliminated simply by operating the blower 16.

[0020]

As described above, according to the present invention, a main body having a culture chamber for accommodating a sample such as cells therein, and a supply device for supplying a gas such as carbon dioxide gas to the culture chamber of the main body,
In a culture cabinet equipped with a passage that connects the upper part and the lower part of the culture chamber, and a blower provided in this passage to circulate the gas in the culture chamber, since a filter is arranged in the passage,
Even if contamination occurs in the culture chamber and the gas in the culture chamber is contaminated with pollutants, it is possible to clean the culture chamber only by filtering the gas with a blower, and the sterilization of the culture chamber is reduced. Work can be eliminated.

[Brief description of drawings]

FIG. 1 is a perspective view of a culture cabinet showing an embodiment of the present invention.

FIG. 2 is a sectional view of the culture cabinet of the present invention.

FIG. 3 is an exploded perspective view showing a state in which a filter is attached to the duct forming the circulation passage of the present invention.

[Explanation of symbols]

 1 Culture Cabinet 5 Main Body 7 Culture Room 8 Supply Device 13 Passage 16 Blower 17 High Performance Filter

Claims (1)

[Claims] 1. A main body having a culture chamber for accommodating a sample such as cells therein, a supply device for supplying a gas such as carbon dioxide gas to the culture chamber of the main body, and an upper portion and a lower portion of the culture chamber are communicated with each other. A culture cabinet provided with a passage and a blower arranged in the passage to circulate gas in the culture chamber, wherein a filter is arranged in the passage, and a pollution control device for the culture cabinet.