JPH06153728A - Method for promoting rooting of hoot of panax ginseng - Google Patents

Abstract

PURPOSE:To easily obtain a large amount of healthy seedlings by extracting a cotyledon from the seed embryo of Panax ginseng, culturing the cotyledon on an MS medium to induce an adventitious embryo, regenerating a shoot from the embryo and culturing the shoot under specific condition. CONSTITUTION:A cotyledon extracted from the seed embryo of Panax ginseng is cultured on an MS medium to directly induce an adventitious embryo. A shoot 1 regenerated from the adventitious embryo is subjected to static culture on an MS medium or B5 medium using vermiculite as the bed material of the medium. The rooting is promoted in a state to suppress the formation of callus 2, the induced root has a number of fine roots 3 and the plant keeps low withering ratio even after acclimatization.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a medicinal carrot shoe.
More specifically, the present invention relates to a method for promoting rooting of callus, and more specifically, in rooting a shoot regenerated from an adventitious embryo, by statically culturing in MS medium or B5 medium using vermiculite as a bed material of the medium, The present invention relates to a method for promoting rooting of shoots of medicinal carrots which suppresses the formation and promotes rooting.

[0002]

BACKGROUND OF THE INVENTION Medicinal carrot is a perennial plant that lives in China, Korea, etc., and is a kind of crude drug raw material that has been widely used as a nourishing tonic or gastrointestinal remedy compounded in Kampo prescriptions. However, since the cultivation period takes as long as 4 to 6 years, the yield is low, and therefore it is very valuable. Therefore, as a method for obtaining a large amount of regenerated plant bodies of medicinal carrots, a method of inducing an adventitious embryo from callus and obtaining a regenerated plant body from this adventitious embryo has been proposed, but in the method of inducing an adventitious embryo from callus, The frequency of occurrence of genetic mutation is high, which causes a defect that a regenerated plant body obtained by culturing an adventitious embryo has a malformation, and there is no suitable method for suppressing this. on the other hand,
A method for accelerating rooting has been attempted by statically culturing shoots regenerated from somatic embryos in MS medium or B5 medium using agar as a bed material for the medium. In this method, as shown in FIG. In culturing the shoot 1 in the above, the formation of the callus 2 is prioritized, and the induced roots and the fine roots 3 are scarce, and a favorable result is not obtained. Moreover, the callus formed during the culture was likely to rot during acclimation, which was one cause of death. Therefore, in order to obtain a large amount of regenerated plants of medicinal carrots, the emergence of a method of promoting rooting while suppressing the formation of callus is desired.

[0003]

In view of the above situation, the present invention has been subjected to various experiments, and as a result, in order to root a shoot regenerated from an adventitious embryo, a bar was used as a floor material of the medium. It was confirmed that rooting was promoted in a state in which callus formation was suppressed when cultured in MS medium or B5 medium using miculite, and based on this confirmation, functionally preferable healthy seedlings were easily and in large quantities. It is an object of the present invention to provide a method for promoting rooting of shoots of medicinal carrots, which can be obtained from

[0004]

[Means for Solving the Problems] In order to solve the above-mentioned problems, a technical means adopted by the present invention is to directly induce adventitious embryos by statically culturing cotyledons isolated from seed embryos of medicinal carrots in MS medium. The soot regenerated from the somatic embryo is then statically cultivated in MS medium or B5 medium using vermiculite as the bed material of the medium.

[0005]

Therefore, according to the present invention, since the cotyledon isolated from the seed embryo of the medicinal carrot was cultured in MS medium, an adventitious embryo with few genetic mutations can be directly induced without undergoing callus formation. Furthermore, by culturing the shoot regenerated from this somatic embryo in MS medium or B5 medium using vermiculite as the bed material of the medium, the influence of harmful substances produced by the culture is reduced and the formation of callus is reduced. Rooting is promoted in a suppressed state, and the number of fine roots is also increased in combination with the air permeability of vermiculite, and functionally preferable healthy seedlings can be obtained.

[0006]

EXAMPLES Examples of the present invention will be described below in detail based on experimental examples. First, the seeds of a medicinal carrot are sterilized with a sterilizing solution such as an aqueous solution of sodium hypochlorite or ethanol, and then the embryos are removed from the seeds with a sterilized scalpel or tweezers, and the cotyledons of the embryos are placed on MS agar medium. Then, somatic embryos were directly induced on the cotyledon surface without passing through callus. In this case, the medium used was MS agar and sucrose-1.
-10% was added, and auxin and cytokinin were further added as plant hormones, but when the concentration of auxin was higher than the concentration of cytokinin, there was a strong tendency to callus, and the number of chromosomes in the cell nucleus increased. Via Callus,
If the concentration of cytokinin is higher than that of auxin, there is a defect that the regenerated plant body obtained by culturing adventitious embryos has a defect that it is easy to increase or decrease and lacks genetic stability, and the regenerated plant body is browned. There is a problem that the tendency to do so becomes stronger. According to the experiment, it was confirmed that the concentrations of auxin and cytokinin are preferably in the range of 0.5 to 2 ppm at substantially the same concentration. Then, when the cotyledons were placed on the medium prepared by the above method and cultivated, after about 2 weeks, protrusion-like ridges were observed on the peripheral surface of the cotyledon, and after one month, 10 to 20 per cotyledon. Somatic embryos were induced.

Next, the somatic embryo was washed with sterile water, and then cytokinin 0.5 to 1.00 ppm and gibberellin 1 to 5
When cultured on MS agar medium containing ppm, shoots were induced from somatic embryos after 30 days. Therefore, M was filled with vermiculite having a size of about 2 to 3 mm square as a floor material for the bottom of the incubator.
When cultured in S medium and B5 medium, as shown in FIG. 2, after 5 to 7 weeks, root formation was promoted in a state in which formation of callus 2 from the shoot was suppressed, and a large number of fine roots 3
Was induced, and functionally preferable healthy seedlings could be obtained.

By the way, Table 1 shows the rooting rate when the shoots regenerated from somatic embryos were cultivated in a medium using agar and a medium using vermiculite as the floor material. . That is, the rooting rate was 30% when agar was used as the floor material in the MS medium, whereas it was 34.2% when vermiculite was used as the floor material, and the floor material was used in the B5 medium. The rooting rate was 60.4% when agar was used, whereas it was 82.6% when vermiculite was used as the floor material. Therefore, as is apparent from the results of this experiment, in order to promote the rooting by culturing the shoot regenerated from the somatic embryo, the medium used was B5 medium and the bottom thereof was filled with vermiculite. It was confirmed that the method of culturing was preferable. It is presumed that the reason is that when vermiculite is used as the floor material, the influence of harmful substances produced by the culture is reduced and the medium is provided with air permeability.

[Table 1]

The plant rooted by the above method is placed on a seedling box (container) in which a non-woven fabric for water supply is laid at the bottom and communicated with a water storage tank, and placed on the upper surface of the non-woven fabric of the above-mentioned seedling box for attachment / detachment. It is acclimatized by a acclimation device including a plug tray connected in a possible state and a cover formed of a light-transmissive film that covers the upper part of the seedling box. That is, the plant is planted in a plug tray to be acclimatized, but the plant rooted by the method of the present invention has a very low mortality rate after acclimatization because callus formation is suppressed, and the plant died at the acclimatization stage. Even if there is such a plant body, since the plug tray is connected in a detachable state, it is possible to avoid adverse effects such as decay on other plants only by removing the relevant portion.

[0010]

In summary, according to the present invention, the cotyledon isolated from the seed embryo of the medicinal carrot is statically cultured in MS medium to directly induce somatic embryos, and the shoots regenerated from the somatic embryos are used as medium. MS using vermiculite as floor material
Since the culture is statically cultivated in the medium or B5 medium, the influence of harmful substances produced by the culture is reduced, and since the culture is performed in an environment in which the medium has aeration, the callus formation is suppressed. Can promote rooting,
The induced roots also have many fine roots, and it is possible to obtain functionally preferable healthy seedlings, and it is possible to significantly reduce the mortality rate even after acclimation, which is a very useful effect.

[Brief description of drawings]

FIG. 1 is an explanatory diagram showing a state in which a shoot regenerated from an adventitious embryo is cultured in a medium using agar as a floor material.

FIG. 2 is an explanatory view showing a state in which a shoot regenerated from an somatic embryo is cultured in a medium using vermiculite as a floor material.

[Explanation of symbols]

 1 shoot 2 callus 3 fine root

Claims (1)

[Claims] 1. A cotyledon isolated from a seed embryo of a medicinal carrot is statically cultured in an MS medium to directly induce an adventitious embryo, and a shoot regenerated from this adventitious embryo is used as a bed material for the medium using vermiculite. Method for promoting root formation of medicinal carrots by static culture in the above-mentioned MS medium or B5 medium.