JPH06138006A - Sample elution device - Google Patents

Abstract

PURPOSE:To provide a sample elution device superior in work efficiency and reliability and capable of automatically eluting sample collected on a sample card into a specific amount of solvent. CONSTITUTION:A solvent is supplied from the bottom of a sheet part 12 holding a sample 13 in the constitution, the solvent and the sample are brought into contact with each other and the elution liquid is discharged again from the bottom of the sheet part 12. As the control of fluid such as the solvent can be done below the sheet part 12, the flow path constitution is simple and thus, a device for eluting sample automatically with a simple constitution can be realized. And by providing an outer lid 14 covering the upper part of the sheet part 12, stain of the device higher part than the sample card 10 is surely prevented. Furthermore, by providing a piston 50 for pushing the upper lid 14, supplying the solvent to lift the piston 50 and storing the energy in a spring 58, the spring 58 is released with a simple action o only switching a three-way valve 80 arranged in a solvent inlet/outlet pipe 42 and the elution liquid can be efficiently dischanged.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to an extremely small amount of explosives, medicines,
The present invention relates to a sample elution device that elutes a sample collected to detect drugs such as pesticides and pollutants into a predetermined amount of solvent.

[0002]

2. Description of the Related Art In recent years, in order to prevent explosion accidents and environmental damage due to explosives and pollutants, trace substances that collect samples from the survey target or the vicinity of the survey target and detect explosives and pollutants The development of detection systems is in progress.

In particular, a fluorescence detection device based on the principle of antigen-antibody reaction has high sensitivity and can detect even suspended matter in the atmosphere, and is highly expected as a device capable of detecting a very small amount of drug.

This fluorescence detection device will be briefly described below. First, a sample is collected on the filter by sucking air and dust around the object to be investigated. This sample is usually in the form of powder and is eluted by mixing with a predetermined amount of solvent in a test tube or the like. This is because the antigen-antibody reaction, which is the measurement principle, is carried out in the liquid.

Next, the obtained eluate is filtered to remove solids such as dust that are not soluble in the solvent, then weighed to a predetermined amount and mixed with a predetermined amount of the antibody solution. When the drug to be detected is present in the eluate, it reacts with the antibody as an antigen.

[0006] An antibody is a substance having a fluorescent reaction, and has the property that its fluorescence intensity changes by binding with an antigen. Therefore, the fluorescence intensity of only the mixed solution of the sample and the antibody and the antibody solution was measured, and when the values of both were compared and a certain rate of change was exceeded, it was determined that the investigation substance was detected in the sample. is there.

It should be noted that dust and the like are removed from the eluate because it itself has a fluorescent reaction and causes an error in measuring the fluorescence intensity.

Here, a series of operations including collection, weighing, mixing, and dust removal until the sample is dissolved in the solvent are all manually performed.

Subsequent operations such as mixing the eluate and the antibody solution, measuring the fluorescence intensity, and the like are carried out in the same manner as a measuring apparatus that handles a plurality of liquids simultaneously by making full use of a pump, a buret, and a valve, which have been widely sold in the past. The work can be easily automated.

[0010]

However, in the above method, there is a problem that the work for eluting the collected sample in the solvent is very complicated and time-consuming, and the efficiency of the entire system is deteriorated.

In consideration of the above problems of the prior art, the present invention provides a sample elution device which can automatically elute a sample collected on a sample card into a predetermined amount of solvent and is excellent in work efficiency and reliability. That is the purpose.

[0012]

According to a first aspect of the present invention, a sample is held on a sheet member which is permeable to a solvent, and the solvent is supplied from the lower side of the sheet member to supply the solvent. It is a sample elution device configured to pass through a sheet member and be eluted to the lower side of the sheet member.

The invention according to claim 2 has a penetrating portion,
A sample card for holding a sample and a solvent-permeable sheet member with the penetrating part blocked, an outer cover for covering the penetrating part of the sample card from the side holding the sample, and a sample card holding sheet A card holding means provided with a cavity facing the member, a solvent supply means for injecting a solvent into the cavity to allow the sheet member to permeate and supply the solvent into the space formed by the outer lid and the sheet member, A sample elution device comprising a discharge means for discharging a solvent and a sample, and after the solvent is injected, the sample permeates the sheet member to be eluted into the cavity and further discharged to the discharge means.

According to a seventh aspect of the present invention, in addition to the configuration of the second aspect of the present invention, the discharging means stores an eluate storage section for storing the solvent and the sample discharged from the cavity, the cavity and the eluate storage. Sample elution device having a pipe line connecting to the elution liquid storage unit, the eluate storage unit is opened to the outside, and the tip of the pipe line is held in a non-contact state with the eluate storage unit. Is.

The invention described in claim 8 is, in addition to the configuration of the invention described in claim 2, provided with a switching means for selectively switching connection between the cavity and the solvent supply means or connection between the cavity and the discharge means. This is a sample elution device.

According to a ninth aspect of the invention, in addition to the configuration of the second aspect of the invention, the sample elution is provided with a seal member crimping means for crimping the seal member around the penetrating portion of the sample card via an outer lid. It is a device.

The eleventh aspect of the present invention is, in addition to the structure of the second aspect, further comprises a piston which is urged by an elastic member to press the outer lid toward the sheet member, and the elastic member is formed by injecting a solvent. After energy is stored in and the solvent is injected, the piston compresses the space formed by the outer lid and the sheet member through the outer lid, the sample permeates the sheet member, is eluted into the cavity, and is further discharged to the discharging means. The sample elution device is configured as described above.

According to a thirteenth aspect of the present invention, in addition to the configuration of the second aspect of the invention, a seal member crimping means for crimping the seal member around the penetrating portion of the sample card through an outer lid and an elastic member are used. A guide for biasing the outer lid toward the sheet member, the seal member crimping means being held openably and closably with respect to the card holding means, and guiding the piston to a part of the seal member crimping means. Part of the piston, and a part of the outer circumference of the piston and a part of the inner circumference of the guide part have substantially the same conical shape, and when the seal member crimping means is open, a part of the outer circumference of the piston and the guide part After the piston is positioned with respect to the seal member crimping means by engaging a part of the circumference, and the seal member crimping means is closed, energy is stored in the elastic member by injecting the solvent and after the solvent is injected. In the sample elution device configured such that the piston compresses the space formed by the outer cover and the sheet member via the outer cover, the sample permeates the sheet member, is eluted into the cavity, and is further discharged to the discharging means. is there.

[0019]

According to the first aspect of the invention, the solvent is supplied from the lower side of the sheet member holding the sample, the solvent and the sample are brought into contact with each other, and the eluate is discharged again to the lower side of the sheet member. Since all the fluids such as solvents can be controlled below the sheet member, the structure of the flow path can be simplified and a device that automatically elutes the sample using a simple mechanism can be realized. You can

According to the second aspect of the present invention, similarly, the solvent is supplied from the lower side of the sheet member holding the sample to the upper side through the cavity by the solvent supply means to allow the sheet member to permeate to bring the solvent into contact with the sample. And elute. At this time, since the sheet member holding the sample is covered with the outer lid from above, the sample and the solvent are prevented from flowing out above the sample card. Therefore, the advancing direction of a fluid such as a solvent can be regulated with a simple structure, and the device above the sample card can be reliably prevented from being contaminated, for example, the sample eluted before can be prevented from being mixed. Therefore, reliable sample elution can always be performed.

According to a seventh aspect of the present invention, the eluent storage unit for storing the solvent and the sample has an opening to the outside, and the tip of the conduit is held in a state of not contacting the eluate storage unit. Therefore, the eluate can be dripped from the tip of the conduit to the bottom surface or the side surface of the eluate storage portion in a non-contact manner. Therefore, it is possible to eliminate bubbles that have penetrated into the pipe line during elution without adhering to the wall surface of the eluate storage section, to reliably quantify the eluate, and to improve the reliability of drug detection. it can.

In the invention described in claim 8, after the solvent supplying means injects the solvent into the cavity to elute the sample, the switching means connects the cavity and the solvent supplying means to the cavity and the discharging means. By selectively switching,
Discharge the solution automatically. That is, the solvent and the sample are naturally dropped from the cavity to the discharging means by their own weight and discharged. Here, for example, if the outer lid is made of an elastic sheet, by injecting a sufficient amount of the solvent into the cavity, the elastic sheet can be inflated and a force can be stored therein. Then, by switching the pipeline to release the load on the solvent supply means, the restraining force of the elastic sheet is released,
The solution can be drained. Therefore, the sample can be easily discharged from the cavity with a simple configuration including only the switching means.

According to the ninth aspect of the present invention, since the seal member is pressed against the periphery of the penetrating portion of the sample card through the outer lid, the outer lid is removed even if the solvent supply load is increased to some extent. It is possible to reliably prevent leakage of the solvent and the like from around the outer lid. Therefore, the space formed by the sheet member and the outer lid is filled with the solvent, and the sample and the solvent are sufficiently brought into contact with each other, whereby reliable elution can be realized. Further, it is possible to prevent contamination due to liquid leakage or the like and further improve the reliability of the device.

According to the eleventh aspect of the present invention, by sufficiently injecting the solvent, the outer lid made of, for example, an elastic sheet and the piston for pressing the outer lid are both pushed up to store energy in the elastic member. Further, the energy of the elastic member is released by switching the pipe line and releasing the load of the solvent supply means, the piston compresses the space formed by the outer lid and the sheet member via the outer lid, and the sample becomes the sheet member. And is eluted into the cavity and discharged to the discharging means. Therefore, the solvent remaining between the outer lid and the sheet member can be minimized, the sample and the solvent can be sufficiently discharged, and the sample can be efficiently eluted with a simple configuration.

In the thirteenth aspect of the present invention, the seal member crimping means is openably and closably held by the card holding means, and a guide portion for guiding the piston is further provided at a part thereof, and a part of the outer circumference of the piston and the inside of the guide portion. Part of the circumference has a conical shape that is almost the same. When the sample card is mounted in the device and the sealing member crimping means is completely closed, the piston contacts the outer lid of the sample card and slides in the guide part, and is urged by the elastic member to the outer lid. Held in. At this time, the engagement between a part of the outer circumference of the piston and a part of the inner circumference of the cylinder is released, and the piston is held slightly rough in the guide portion. Therefore, the movement of the piston that moves up and down in the process of injecting and discharging the solvent becomes smooth, and the tip of the piston can be easily aligned with the inner peripheral surface of the penetrating portion of the sample card.
It is possible to surely elute the sample. Further, when the seal member crimping means is opened and the sample card is taken out of the apparatus, the piston urged by the elastic member is guided by the cone portion on the outer periphery of the piston to the cone portion provided on the inner periphery of the cylinder portion. Part of the inner circumference of the guide part is engaged again,
It is possible to accurately hold the piston biased by the elastic member in a state of slightly protruding in the center of the guide portion. Therefore, when the next sample card is mounted and the seal member crimping means is closed, the tip of the piston and the penetrating portion of the sample card can be reliably positioned.

[0026]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below with reference to the drawings showing its embodiments.

First, the structure of the sample card and the method of collecting the sample on the sample card will be described.

6 and 7 are a plan view and a sectional view of the sample card 10. The sample card 10 includes a holding plate 11, a sheet member 12, a sample 13, and an outer lid 14.
The holding plate 11 is made of ABS resin, for example, and has a vertical size of 6
4 mm x 40 mm x thickness 3 mm, with a diameter of 2
It has a penetration portion 15 of 0 mm.

The sheet member 12 is, for example, 8 of SUS316.
It is made of 00 mesh, and is held substantially parallel to the surface of the holding plate 11 at approximately the center of the through portion 15 in the thickness direction. The penetrating portion 15 is provided with a conical taper portion 16 having a predetermined apex angle on the upper side of the sheet member 12 as a boundary.

As a method of manufacturing such a sample card 10, as shown in FIG. 7, a through hole 15 is provided with a counterbore 17 having a diameter of 26 mm and a depth of 2 mm, and a sheet member 12 is mounted on the through hole 15, and then a taper portion is formed on the inner circumference. A method in which a ring 18 having 16 is fitted and they are integrally welded by heating or using a solvent is considered. The cross section of the tapered portion 16 forms an inclined surface of 45 degrees, and the maximum inner diameter of the ring 18 is 24 mm.

The outer lid 14 is an elastic sheet made of silicon rubber or the like, and an adhesive is applied to a part thereof. After the sample 13 is collected and held on the sheet member 12, the outer lid 14 is bonded so as to cover the entire surface of one side of the penetrating portion 15, so that the sample 13 is enclosed in the sample card 10. At this time, the sample 13 is collected and held on the upper side of the sheet member 12, that is, where the penetrating portion 15 has the tapered portion 16.

Incidentally, 19a and 19b are the sample cards 1
These are positioning long holes and holes used when 0 is mounted in the sample elution device described later.

FIG. 8 is a block diagram of a sample collecting device 20 for collecting the sample 13 on the sample card 10. The sample collecting device 20 includes a sample card 10 and a cassette holder 21.
And air suction means 22.

Further, the cassette holder 21 comprises a cassette insertion portion 23 for inserting and holding the sample card 10, and a nozzle 24a and a suction pipe line 24b which face each other with the penetrating portion 15 of the mounted sample card 10 interposed therebetween. . The air suction means 22 is connected to the suction pipe line 24b, and the sample card 1
No. 0 sheet member 12 and the nozzle 24a communicate with the outside.

When collecting a sample, the sample card 10 is mounted on the cassette holder 21 (direction of arrow X), and external air is sucked from the nozzle 24a by the air suction means 22 (direction of arrow Y). On the sheet member 12 of the sample card 10, dust or dust larger than the mesh-shaped hole is captured, and the sample 13 for detecting the presence or absence of the drug is captured.

Next, a sample elution device according to an embodiment of the present invention will be described with reference to the drawings. FIG. 1 shows the configuration of a sample elution device according to an embodiment of the present invention.

In FIG. 1, 10 is a sample card, 30 is an elution section, 70 is a solvent supply means, 80 is a three-way valve, and 90.
Is an eluate reservoir.

The elution section 30 is composed of a lower unit 31 for holding the sample card 10 and an upper unit 32 for pressing and holding the sample card 10 to the lower unit 31. The upper unit 32 is rotatably attached to the lower unit 31, and is openable / closable to facilitate attachment / detachment of the sample card 10 and cleaning of each part.

The lower unit 31 includes a cassette holder 40, a cavity 41 provided on the cassette holder 40, and a solvent inlet / outlet conduit 4.
2, O-ring 43, positioning pins 44a, 44b, 44
c, 44d (not shown).

The cavity 41 has a diameter of 20 mm and a depth of 2 mm.
Is a conical recess (volume: 0.2 cm ³ ) of which the solvent inlet / outlet conduit 42 communicating with the outside has a diameter of about 1 mm.
Is provided. In addition, the O-ring 43 is the cavity 4
It is attached to the outer periphery of the upper part of 1.

Positioning pins 44 on the cassette holder 40
The a and 44b engage with the elongated holes 19a and 19b for positioning the sample card 10 shown in FIG. 6, respectively, and position the sample card 10 with respect to the cavity 41.

The upper unit 32 is mainly composed of a piston 50, a piston holder 51 and an outer frame 52. The piston 50 is held slidably in the vertical direction in a guide portion 53 provided in a piston holder 51, and is biased against the sample card 10 by a pressing spring 58. However, in order to avoid complication of the drawing, in FIG. 1, the piston 50 is shown in a slightly raised state.

Further, the piston holder 51 has an outer frame 5
3 guide rods 54 implanted in 2 (only 1 shown)
Is slidable in the vertical direction, and is urged downward by a spring 55.

An O-ring 56 is attached to the outer periphery of the guide portion 53 on the lower surface of the piston holder 52. The O-ring 56 is pressed against the upper surface of the sample card 10 via the outer lid 14 by the force of the pressing spring 55, and at the same time, the sample card 10 is pressed.
The O-ring 43 on the lower unit 31 is crimped.

The piston holder 51 has positioning holes 57a and 57b (not shown) which are engaged with positioning pins 44c and 44d (not shown) on the lower unit 31 for positioning. .

The outer diameter of the piston 50 is 25 mm, and a taper portion 63 is provided on the outer circumference of the tip end portion thereof. The cross section of the taper portion 63 forms an inclined surface of 45 degrees, and the diameter of the tip surface 66 is 21 mm. The taper portion 63 is guided to the taper portion 16 of the sample card 10 via the outer lid 14, and the piston 50 is positioned substantially at the center of the penetrating portion 15 of the sample card 10.

Here, the diameter (21 mm) of the tip surface 66 of the piston 50 is equal to the diameter (2 mm) of the penetrating portion 15 of the sample card 10.
Since it is set to be slightly larger than 0 mm), the taper portion 63 of the piston 50 always contacts the taper portion 16 of the sample card 10 via the outer lid 14. Therefore, the piston 50 does not press the sheet member 12.

This is due to the force of the spring 58.
This is to prevent the sample 13 from flowing out due to deformation / damage of the sample 2 and to efficiently compress the space formed by the outer lid 14 and the sheet member 12 by the piston 50.

A piston shaft 59 is implanted in the center of the piston 50, and the upper end of the piston shaft 59 is attached to the piston holder 5.
One of them is projected to the outside through a hole 61 provided in the top plate 60. An E-ring 62 is attached to this protrusion to prevent the piston 50 from falling off when the upper and lower units are opened.

The outer diameter of the piston 50 is 25 mm as described above, but the outer diameter is 27 mm on the upper side by providing the tapered portion 64. The guide portion 53 that guides the piston 50 also has an inner diameter of 27 mm, but a tapered portion 65 is provided so that the inner diameter is 29 mm on the upper side.

In the state shown in FIG. 1, the piston 50
Since there is a diameter difference of 2 mm between the guide part 53 and
The piston 50 is easily guided and positioned by the tapered portion 16 of the sample card 10.

Also, with the upper and lower units open,
The piston 50 protrudes from the guide portion 53 by the spring 58, and at this time, the piston 50 has its upper end outer periphery at the guide portion 53.
Is guided to the inner circumference of the lower end of. Therefore, when closing the upper and lower units, the piston 50 protruding from the guide portion 53
Is guided almost in the center of the tapered portion 16 of the sample cassette 10 to facilitate positioning.

The solvent supply means 70 supplies a predetermined amount of solvent into the cavity 41 through the solvent inlet / outlet conduit 42. The three-way valve 80 connects the cavity 41 and the solvent supply means 70 through the solvent inlet / outlet conduit 42, and the other one is connected to the discharge conduit 81. The three-way valve 80 is driven according to the input of the control signal,
The connection between the cavity 41 and the solvent supply means 70 or between the cavity 41 and the discharge conduit 81 is selectively switched.

The eluate storage unit 90 is, for example, a cell (capacity -3 cm ³ ) made of Teflon having excellent water repellency, and stores the eluate sent through the discharge conduit 81. The eluate storage unit 90 has an opening 91 for the outside air. Further, the tip of the discharge conduit 81 is held in a non-contact state with the bottom surface and the side surface of the eluate storage unit 90, and a discharge conduit 92 is further provided on the bottom surface.

The operation of the sample elution device configured as described above will be described with reference to FIGS. 1 and 2. Figure 2
It is a figure showing the sample elution device of this invention in the state which opened the upper unit 32.

When mounting the sample card 10, FIG.
The upper unit 32 is rotated to open the lower unit 31, as shown in FIG.
9b is engaged with the positioning pins 44a and 44b, and the sample card 10 is positioned so that the center of the penetrating portion 15 and the center of the cavity 41 are substantially aligned with each other.

At this time, the piston holder 51 has the spring 5
It is urged downward by 5. Further, the piston 50 is pressed by the spring 58 and its tip is projected from the guide portion 53. However, since the outer periphery of the upper end is guided to the inner periphery of the lower end of the guide portion 53, as described above,
It is guided to the center of 3.

When the upper unit 32 is closed, as shown in FIG.
By engaging 7a and 57b (not shown) with the positioning pins 44c and 44d (not shown) on the lower unit 31, the center of the guide portion 53 and the center of the penetrating portion 15 of the sample card 10 are substantially formed. Positioned to match. Further, the piston 50 guided in the center of the guide portion 53 is also guided almost in the center of the taper portion 16 of the sample cassette 10 and easily positioned.

The piston 50 is the outer lid 14 of the sample card 10.
The outer lid 14 is held in a state of being urged by the spring 58 by the spring 58. At this time, a part of the outer circumference of the piston 50 is disengaged from a part of the inner circumference of the guide part 53, and the piston 50 is held in the guide part 53 slightly rough.

Next, FIG. 3 shows the actual sample elution operation.
And FIG. 4 will be described in more detail. Note that FIG. 3 is an operation explanatory view of the sample elution device showing a state in which the solvent is supplied into the cavity 41 and FIG. 4 is a state in which the eluate is discharged through the discharge conduit 81.

First, the solvent is supplied by the solvent supply means 70 through the solvent inlet / outlet conduit 42 and the three-way valve 80 to the cavity 41.
Supply in. After the solvent fills the cavity 41,
After passing through the sheet member 12 of the sample card 10 and contacting the sample 13, elution is performed. When the solvent is further injected, as shown in FIG. 3, the outer lid 14 and the piston 50 that presses the outer lid 14 are both pushed up to store energy in the spring 58.

Since the sheet member 12 holding the sample is covered with the outer lid 14 from above, the sample and solvent are prevented from flowing out above the sample card 10. Further, the O-ring 56 provided under the piston holder 51 is attached to the outer lid 1.
Since it is pressure-bonded to the periphery of the penetrating portion 15 of the sample card 10 via 4, it is possible to surely remove the outer lid 14 or leak the solvent from the periphery of the outer lid 14 even if the solvent supply load is increased to some extent. Can be prevented.

Next, as shown in FIG. 4, the three-way valve 8
When the connection between the cavity 41 and the solvent supply means 70 is cut by 0 and the pipeline is switched so that the cavity 41 and the discharge pipeline 81 are connected, the solvent supply load on the cavity 41 of the solvent supply means 70 is released, The energy stored in the spring 58 is released.

Then, the piston 50 compresses the space formed by the outer lid 14 and the sheet member 12 via the outer lid 14, and the sample permeates the sheet member 12 to form the cavity 41.
It is eluted inside and sent to the discharge line 81 through the solvent inlet / outlet line 42 and the three-way valve 80.

Further, the eluate is dropped from the tip of the discharge conduit 81 to the bottom surface or the side surface of the eluate storage section 90 having an opening to the outside air in a non-contact manner. Here, the bubbles that have entered the discharge conduit 81 during elution can be removed to the outside without adhering to the wall surface of the eluate storage section 90.

In this way, the eluate obtained by supplying the solvent to the sample on the sheet member 12 from the lower side and further discharging it to the lower side is stored in the eluate storage section 90.

When the upper unit 32 is opened and the sample card 10 after elution is taken out of the apparatus, the piston 50 urged by the spring 58 has the taper 64 on the outer periphery thereof provided on the inner periphery of the guide portion 53. Guided by the taper 65, a part of the outer periphery of the piston 50 and a part of the inner periphery of the guide portion 53 are engaged again, and the piston 50 urged by the spring 58 is guided to the guide portion 5
It can be held with high precision in a state of being slightly projected to the center of 3.

Therefore, when the next sample card 10 is mounted and the upper unit 32 is closed again, the tip of the piston 50 and the penetrating portion 15 of the sample card 10 can be reliably positioned.

As described above, according to the present embodiment, since the fluid control of the solvent or the like can be performed entirely below the sheet member 12, the flow channel structure is simplified and a simple mechanism is used. It is possible to realize a device that automatically elutes a sample.

Further, at the time of elution, the piston 50 has the guide portion 53.
Since the piston 50 is held slightly rough inside, the movement of the piston 50 that moves up and down in the process of solvent injection and discharge becomes smooth, and the taper 63 at the tip of the piston 50 becomes the taper 16 at the inner circumference of the penetrating portion 15 of the sample card 10. On the other hand, the sample can be easily placed along the line, and the sample can be surely eluted.

Further, since the outer peripheral wall surface of the penetrating portion 15 of the sample card 10 and the tip of the piston 50 both have a conical shape with the same apex angle, even if the relative positions of the sample card 10 and the piston 50 are slightly deviated, Stable positioning can be performed, and the eluate of the sample can be surely eluted by minimizing the residual amount of the eluate, and the shearing force applied to the outer lid 15 is mitigated to prevent the outer lid 15 from breaking and resulting liquid leakage. can do.

Next, a fluorescence detection device using the sample elution device of the present invention will be briefly described with reference to FIG.

In FIG. 5, as described above, 10 is a sample card, 30 is an elution section, 70 is a solvent supply means, 80 is a three-way valve, and 90 is an eluate storage section. Further, 93 is an eluate weighing means, 94 is a measuring cell, 95 is a fluorescence measuring means,
96 is an antibody liquid supply means, and 97 is a waste liquid tank.

The eluate stored in the eluate storage unit 90 by the apparatus and method described above is measured by the eluate weighing means 93.
Is weighed to a predetermined amount and sent to the measuring cell 94 in the fluorescence measuring means 95. Then, it is mixed with a predetermined amount of antibody solution sent by the antibody solution supply means 96.

Next, as in the conventional example, the fluorescence measuring means 95 is used.
Measure the fluorescence intensity of this mixed solution by comparing with the fluorescence intensity of the antibody solution measured in advance, and if the value exceeds a certain rate of change, the antigen that reacts with the antibody in the sample It is judged that the substance that becomes

Finally, the mixed liquid in the measuring cell 94 is discharged to a waste liquid tank 97 by a waste liquid pump (not shown),
A series of work is completed.

After the measurement, the eluate remaining in the device was removed, and each part of the device through which the eluate passed was washed,
It is necessary to prepare for the next sample measurement. This residual liquid removal and cleaning is performed by supplying only the solvent to each part by the solvent supply means 70, sufficiently diluting the eluate, and finally collecting in the waste liquid tank 97.

Therefore, by constructing a fluorescence detection device utilizing such an antigen-antibody reaction using the sample elution device of the present invention, it is only necessary to mount a sample card in which a sample is collected on a sheet member to the device. Not only can the sample on the sheet member be automatically eluted into the solvent with a simple operation,
It is possible to provide a trace amount drug detection system which is completely automated and has excellent work efficiency and reliability.

[0079]

As is apparent from the above description, the present invention provides a sample elution device which automatically elutes the sample collected on the sample card into a predetermined amount of solvent and is excellent in work efficiency and reliability. can do.

[Brief description of drawings]

FIG. 1 is a configuration diagram of a sample elution device according to an embodiment of the present invention.

FIG. 2 is a configuration diagram of a sample elution device according to an embodiment of the present invention.

FIG. 3 is an operation explanatory diagram of a sample elution device according to an embodiment of the present invention.

FIG. 4 is an operation explanatory diagram of a sample elution device according to an embodiment of the present invention.

FIG. 5 is a configuration diagram of a fluorescence detection device using a sample elution device according to an embodiment of the present invention.

FIG. 6 is a plan view of a sample card of a sample elution device according to an embodiment of the present invention.

FIG. 7 is a sectional view of a sample card of the sample elution device in the example.

FIG. 8 is a configuration diagram of a sample collection device that collects a sample on a sample card of the sample elution device in the example.

[Explanation of symbols]

 10 sample card 12 sheet member 13 sample 14 outer lid 15 penetrating part 16, 63 taper part 30 elution part 40 card holder 41 cavity 42 solvent inlet / outlet conduit 43, 56 O-ring 44c, 44d positioning pin 50 piston 51 piston holder 53 guide Part 58 Spring 64, 65 Taper 70 Solvent supply means 80 Three-way valve 81 Discharge pipe line 90 Eluent storage part

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Hitoshi Miyazaki, 1006 Kadoma, Kadoma City, Osaka Prefecture Matsushita Electric Industrial Co., Ltd.

Claims (13)

[Claims] 1. A sample is held on a sheet member which is permeable to a solvent, the sheet member is permeated from the lower side of the sheet member to supply the solvent, and the sample permeates the sheet member to form the sheet. A sample elution device, which is configured to elute below a member. 2. A sample card having a penetrating portion, which holds a sample and holds a solvent and which holds a solvent in a state in which the penetrating portion is closed, and the penetrating portion of the sample card holds the sample. An outer lid that covers from the side, a card holding means that holds the sample card and has a cavity facing the sheet member, and a solvent is injected into the cavity to allow the sheet member to pass therethrough and the outer lid and the sheet. A solvent supply means for supplying the solvent into the space formed by the member and a discharge means for discharging the solvent and the sample in the cavity are provided, and the sample permeates the sheet member after the solvent is injected. And then eluted into the cavity,
Further, the sample elution device is configured to be discharged to the discharging means. 3. The sample elution device according to claim 2, wherein the sheet member has a mesh shape. 4. The sample elution device according to claim 2, wherein the outer lid is an elastic sheet. 5. The sample elution device according to claim 2, wherein an outer lid is attached to the sample card body at least when the sample card is outside the sample elution device. 6. The sample elution device according to claim 2, wherein the discharging means is provided with an opening / closing means for controlling connection with the cavity. 7. The discharging means comprises an eluate storage section for storing the solvent and the sample discharged from the cavity, and a pipe line connecting the cavity and the eluate storage section. The sample elution device according to claim 2, wherein the sample elution device is open to the outside, and the tip of the conduit is held in a non-contact state with the eluate storage unit. 8. A sample card having a penetrating portion, which holds a sample and holds a solvent and which holds a solvent in a state in which the penetrating portion is closed, and the penetrating portion of the sample card holds the sample. An outer lid that covers from the side, a card holding means that holds the sample card and has a cavity facing the sheet member, and a solvent is injected into the cavity to allow the sheet member to pass therethrough and the outer lid and the sheet. Solvent supply means for supplying the solvent into the space formed by the member, discharge means for discharging the solvent and the sample in the cavity, connection between the cavity and the solvent supply means, or the cavity and the discharge Switching means for selectively switching the connection with the means, after the injection of the solvent, the sample permeates the sheet member and is eluted into the cavity, Sample elution apparatus characterized by being configured so as to be discharged to the discharge means al. 9. A sample card having a through-hole, holding a sample and holding a solvent-permeable sheet member in a state in which the through-hole is closed, and the through-hole of the sample card holds the sample. An outer lid that covers from the side, a card holding means that holds the sample card and has a cavity facing the sheet member, and a solvent is injected into the cavity to allow the sheet member to pass therethrough and the outer lid and the sheet. A solvent supply means for supplying the solvent into a space formed by a member, a discharge means for discharging the solvent and the sample in the cavity, and a seal member for mounting the outer lid around the penetrating portion of the sample card. And a sealing member crimping means for crimping via the solvent. After the solvent is injected, the sample permeates the sheet member to be eluted into the cavity and further discharged to the discharging means. Sample elution apparatus characterized by being configured as. 10. The seal member crimping means is held openably and closably with respect to the card holding means, and in the closed state, the sealing member crimping means is positioned by a positioning member provided in the card holding means. The sample eluting apparatus according to claim 9, which is characterized in that. 11. A sample card having a through-hole, holding a sample and holding a solvent-permeable sheet member in a state in which the through-hole is closed, and the through-hole of the sample card holds the sample. An outer lid that covers from the side, a card holding means that holds the sample card and has a cavity facing the sheet member, and a solvent is injected into the cavity to allow the sheet member to pass therethrough and the outer lid and the sheet. Solvent supply means for supplying the solvent into the space formed by the member, discharge means for discharging the solvent and the sample in the cavity, and the outer lid urged by an elastic member toward the sheet member. And a piston for pressing, storing energy in the elastic member by the injection of the solvent,
After the injection of the solvent, the piston compresses the space formed by the outer lid and the sheet member through the outer lid, the sample permeates the sheet member and is eluted into the cavity, and A sample elution device, which is configured to be discharged to a discharging means. 12. A taper portion having substantially the same conical shape is provided on each of a part of a penetrating portion of a sample card and a tip end portion of a piston, and before the piston comes into contact with a sheet member via an outer lid, The sample elution device according to claim 11, wherein the tapered portion of the piston is configured to abut the tapered portion of the penetrating portion of the sample card. 13. A sample card having a through-hole, holding a sample and holding a solvent-permeable sheet member in a state where the through-hole is closed, and the through-hole of the sample card holds the sample. An outer lid that covers from the side, a card holding means that holds the sample card and has a cavity facing the sheet member, and a solvent is injected into the cavity to allow the sheet member to pass therethrough and the outer lid and the sheet. A solvent supply means for supplying the solvent into a space formed by a member, a discharge means for discharging the solvent and the sample in the cavity, and a seal member for mounting the outer lid around the penetrating portion of the sample card. A seal member crimping means for crimping via the seal member, and a piston biased by an elastic member to press the outer lid toward the sheet member. A guide part for guiding the piston is formed in a part of the seal member crimping part while being openably and closably held by the card holding means, and a part of the outer circumference of the piston and the inner circumference of the guide part. A part of the same has a substantially conical shape, and in a state where the seal member crimping means is opened, a part of the outer circumference of the piston and a part of the inner circumference of the guide part engage with each other to the seal member crimping means After the piston is positioned and the sealing member crimping means is closed, energy is stored in the elastic member by injecting the solvent, and after the solvent is injected, the piston is connected to the outer lid via the outer lid. The space formed by the sheet member is compressed so that the sample permeates the sheet member, is eluted into the cavity, and is further discharged to the discharging means. Samples eluted and wherein the.