JPH06133758A - Injection device preventing sedimentation - Google Patents
Injection device preventing sedimentationInfo
- Publication number
- JPH06133758A JPH06133758A JP4284252A JP28425292A JPH06133758A JP H06133758 A JPH06133758 A JP H06133758A JP 4284252 A JP4284252 A JP 4284252A JP 28425292 A JP28425292 A JP 28425292A JP H06133758 A JPH06133758 A JP H06133758A
- Authority
- JP
- Japan
- Prior art keywords
- suspension
- cylinder
- plunger
- blade
- pouring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、比重差のある粒子成分
を含む懸濁液を注入器（シリンジ）を介して遅い速度で
注出する装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device for pouring a suspension containing particle components having different specific gravities through a syringe at a slow speed.
【０００２】[0002]
【従来の技術】例えば、医薬品の研究や製造技術、およ
び医薬品の研究用実験装置の開発分野等においては、細
胞培養時の培養槽へ細胞を混ぜた懸濁液を注入したり、
電気泳動装置の泳動槽に細胞やタンパク質または染色体
などの試料を混ぜた懸濁液を自動的に注入するなどの作
業が行われている。2. Description of the Related Art For example, in the field of drug research and manufacturing technology, and the field of development of experimental devices for drug research, injecting a suspension containing cells into a culture tank at the time of cell culture,
Work such as automatically injecting a suspension obtained by mixing a sample such as cells, proteins or chromosomes into a migration tank of an electrophoresis apparatus is performed.
【０００３】このような懸濁液の注入は注出器（シリン
ジ）を用いて行われるが、注出器はシリンダとプランジ
ャとで構成され、このシリンダ内に収容した懸濁液（溶
液）をプランジャにより押し出すようになっている。Injection of such a suspension is performed using a pour-out device (syringe). The pour-out device is composed of a cylinder and a plunger, and the suspension (solution) contained in this cylinder is used. It is designed to be pushed out by a plunger.
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、この種
の注出器は懸濁液の送り出し量が少なく、例えば数ｃｃ
／min のレベルで注出することがある。すなわち、シリ
ンダ内に収容した懸濁液を全部送り出すのに数分の時間
を要する。However, this type of dispenser has a small amount of suspension to be delivered, for example, several cc.
May be dispensed at a level of / min. That is, it takes several minutes to send out all the suspension contained in the cylinder.
【０００５】このため、懸濁液はシリンダ内に長時間と
どまり、これは懸濁液が静止に近い状態に置かれること
になるから、懸濁液中の粒子成分、例えば上記細胞やタ
ンパク質または染色体などのように比重の重い混合物が
沈殿してしまい、粒子成分の混合濃度が薄い溶液が注出
されることになる。This causes the suspension to remain in the cylinder for an extended period of time, which leaves the suspension in a near-quiescent state, so that the particle components in the suspension, such as the cells, proteins or chromosomes mentioned above. As described above, a mixture having a high specific gravity is precipitated, and a solution having a small concentration of mixed particle components is poured out.
【０００６】本発明はこのような事情にもとづきなされ
たもので、その目的とするところは、シリンダ内に収容
した懸濁液を攪拌して沈降を防止することができる沈降
防止注出装置を提供しようとするものである。The present invention has been made in view of the above circumstances. An object of the present invention is to provide an anti-settling pouring device capable of stirring a suspension contained in a cylinder to prevent sedimentation. Is what you are trying to do.
【０００７】[0007]
【課題を解決するための手段】本発明は上記の目的を達
成するため、比重差のある粒子成分を含む懸濁液をシリ
ンダおよびプランジャを用いて注出する装置において、
上記シリンダ内に羽根を収容するとともに、この羽根を
外部から回転駆動する手段を有し、上記羽根の回転によ
り上記シリンダ内の懸濁液を攪拌することを特徴とす
る。In order to achieve the above object, the present invention provides an apparatus for pouring a suspension containing particle components having a difference in specific gravity using a cylinder and a plunger.
The present invention is characterized by accommodating blades in the cylinder and having means for rotationally driving the blades from the outside, and stirring the suspension in the cylinder by rotation of the blades.
【０００８】[0008]
【作用】本発明によれば、シリンダ内に収容した羽根を
回転させるとシリンダ内の懸濁液を攪拌し、粒子成分の
沈降を防止して懸濁液を均等な濃度に保つことができ
る。According to the present invention, when the blades housed in the cylinder are rotated, the suspension in the cylinder is agitated to prevent the settling of the particle components and keep the suspension at a uniform concentration.
【０００９】[0009]
【実施例】以下本発明について、図示の一実施例にもと
づき説明する。本実施例は、細胞やタンパク質が混合さ
れた懸濁液を細胞培養槽へ注出する装置の例を示し、図
３はその自動注出装置の全体の構造を示す。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below with reference to the illustrated embodiment. This embodiment shows an example of an apparatus for pouring a suspension in which cells and proteins are mixed into a cell culture tank, and FIG. 3 shows the entire structure of the automatic pouring apparatus.
【００１０】図３において、１は駆動モータであり、こ
の駆動モータ１の回転は減速ギア列２１、２２、２３、
２４、２５および２６を介してクラッチ３に伝えられ
る。クラッチ３は、駆動側３１と従動側３２との間の通
断をなすもので、従動側３２には従動軸４を連結してあ
る。よって、このクラッチ３をつなぐと、前記駆動モー
タ１の回転が減速されて従動軸４に伝えられるようにな
っている。In FIG. 3, reference numeral 1 is a drive motor, and the rotation of the drive motor 1 is performed by the reduction gear trains 21, 22, 23,
It is transmitted to the clutch 3 via 24, 25 and 26. The clutch 3 connects and disconnects the driving side 31 and the driven side 32, and the driven shaft 32 is connected to the driven side 32. Therefore, when the clutch 3 is engaged, the rotation of the drive motor 1 is decelerated and transmitted to the driven shaft 4.
【００１１】従動軸４の途中にはボールねじ式送り機構
を構成する送りねじ５が形成されており、この送りねじ
５には、送りナット６が螺合されている。上記従動軸４
が回転すると、上記送りナット６は軸方向に直線移動さ
れる。また、上記従動軸４の先端部には平歯車（駆動
側）７が一体に回転するように取付けられている。A feed screw 5 constituting a ball screw type feed mechanism is formed in the middle of the driven shaft 4, and a feed nut 6 is screwed into the feed screw 5. The driven shaft 4
When is rotated, the feed nut 6 is linearly moved in the axial direction. A spur gear (drive side) 7 is attached to the tip of the driven shaft 4 so as to rotate integrally therewith.
【００１２】上記送りナット６および平歯車７は、注出
器１０を作動する。注出器１０の詳細な構造は、図１お
よび図２に示されているので、これらを参照しながら説
明する。The feed nut 6 and the spur gear 7 operate the dispenser 10. The detailed structure of the dispenser 10 is shown in FIGS. 1 and 2, and will be described with reference to these.
【００１３】注出器１０は、シリンダ１１と、このシリ
ンダ１１内に摺動自在に嵌挿されたプランジャ１２を有
している。シリンダ１１は先端部に注出ノズル１３を有
し、シリンダホルダ１４によって水平に支持されてい
る。プランジャ１２はステム１５を有し、このステム１
５は上記送りナット６に連結されている。よって、送り
ナット６が軸方向に移動するとプランジャ１２がシリン
ダ１１内で軸方向に摺動する。プランジャ１２が図示の
左方向に移動する場合に、シリンダ１１内に収容した懸
濁液を押し、この懸濁液を注出ノズル１３から細胞培養
槽（図示しない）へ送り出す。The dispenser 10 has a cylinder 11 and a plunger 12 slidably fitted in the cylinder 11. The cylinder 11 has a pouring nozzle 13 at its tip and is horizontally supported by a cylinder holder 14. The plunger 12 has a stem 15 and the stem 1
5 is connected to the feed nut 6. Therefore, when the feed nut 6 moves in the axial direction, the plunger 12 slides in the cylinder 11 in the axial direction. When the plunger 12 moves to the left in the figure, the suspension contained in the cylinder 11 is pushed, and the suspension is delivered from the pouring nozzle 13 to a cell culture tank (not shown).
【００１４】この場合、プランジャ１２の移動速度は、
前記モータ１の回転速度、減速ギア列の変速比、送りね
じ５のねじピッチ等を適宜選択して極めてゆっくりした
速度で移動するように設定されており、例えば注出ノズ
ル１３から送り出される懸濁液の注出量が数ｃｃ／min
となるように微低速に設定されている。In this case, the moving speed of the plunger 12 is
The rotation speed of the motor 1, the gear ratio of the reduction gear train, the screw pitch of the feed screw 5 and the like are appropriately selected so that the motor 1 is set to move at an extremely slow speed. Liquid pouring rate is several cc / min
It is set to a very low speed so that
【００１５】上記プランジャ１２の先端には羽根１６が
回転自在に取付けられている。この羽根１６は磁石また
は磁性体により形成されており、周囲の磁界が変化する
と回転するようになっている。このような羽根１６に対
し、シリンダ１１の外側には、平歯車（従動側）８が軸
受９を介して回転自在に取付けられている。この従動側
平歯車８は前記駆動側平歯車７に噛合っており、よって
従動軸４が回転すると、この駆動側平歯車７を介して従
動側平歯車８が回転されるようになっている。この従動
側平歯車８の内面には、磁石１８、１８が取付けられて
おり、従動側平歯車８が回転すると磁石１８、１８がシ
リンダ１１の周囲を回転するようになっている。このよ
うな構成による注出装置の作用を説明する。A blade 16 is rotatably attached to the tip of the plunger 12. The blade 16 is made of a magnet or a magnetic material, and rotates when the surrounding magnetic field changes. A spur gear (driven side) 8 is rotatably attached to the outside of the cylinder 11 with respect to the blade 16 through a bearing 9. The driven spur gear 8 meshes with the drive side spur gear 7, so that when the driven shaft 4 rotates, the driven spur gear 8 is rotated via the drive side spur gear 7. . Magnets 18, 18 are attached to the inner surface of the driven spur gear 8, and when the driven spur gear 8 rotates, the magnets 18, 18 rotate around the cylinder 11. The operation of the pouring device having such a configuration will be described.
【００１６】注出器１０のシリンダ１１内に、図示しな
い供給装置から懸濁液を注入する。懸濁液としては、例
えばＨＥＰＰＳ、ＨＥＰＥＳ（いずれも薬品の商品
名）、グリシンおよび硝酸カリウムなどを混合してなる
比重が１．０の緩衝溶液に、比重が１を越え２未満の細
胞およびタンパク質を混ぜた混合液とする。シリンダ１
１内に所定量の懸濁液を充填したならば、駆動モータ１
を回転させ、クラッチ３をつなぐ。The suspension is injected into the cylinder 11 of the dispenser 10 from a supply device (not shown). As a suspension, for example, HEPPS, HEPES (all are trade names of chemicals), glycine, potassium nitrate and the like are mixed in a buffer solution having a specific gravity of 1.0, and cells and proteins having a specific gravity of more than 1 and less than 2 are added. Use the mixed solution. Cylinder 1
If a predetermined amount of suspension is filled in the drive motor 1,
Rotate and connect the clutch 3.
【００１７】すると、駆動モータ１の回転が減速ギア列
２１、２２、２３、２４、２５、２６およびクラッチ３
を介して従動軸４に伝えられ、この場合従動軸４の回転
速度は駆動モータ１の回転速度に比べて大幅に減速され
る。Then, the rotation of the drive motor 1 causes the reduction gear trains 21, 22, 23, 24, 25, 26 and the clutch 3 to rotate.
The rotation speed of the driven shaft 4 is significantly reduced as compared with the rotation speed of the drive motor 1 in this case.
【００１８】従動軸４が回転すると、送りねじ５の回転
により送りナット６が図示左方向に直線移動される。こ
の送りナット６は、ステム１５を通じてプランジャ１２
を軸方向に押すので、プランジャ１２はシリンダ１１内
に収容した懸濁液を押す。よって、懸濁液の圧力が高く
なり、この懸濁液は注出ノズル１３を通じて細胞培養槽
（図示しない）へ送り出される。When the driven shaft 4 rotates, the feed screw 6 rotates to linearly move the feed nut 6 to the left in the drawing. The feed nut 6 is attached to the plunger 12 through the stem 15.
Is pushed in the axial direction, the plunger 12 pushes the suspension contained in the cylinder 11. Therefore, the pressure of the suspension increases, and the suspension is sent out to the cell culture tank (not shown) through the pouring nozzle 13.
【００１９】このようなプランジャ１２の押圧作動中、
上記従動軸４に取着された駆動側平歯車７が回転するか
ら、この駆動側平歯車７に噛合っている従動側平歯車８
も回転する。よって、従動側平歯車８の内面に取着した
磁石１８、１８がシリンダ１１の周囲を回転し、羽根１
６の周囲の磁界が変化される。このため羽根１６が回転
する。During such pressing operation of the plunger 12,
Since the drive side spur gear 7 attached to the driven shaft 4 rotates, the driven side spur gear 8 meshed with the drive side spur gear 7
Also rotates. Therefore, the magnets 18, 18 attached to the inner surface of the driven side spur gear 8 rotate around the cylinder 11, and the blade 1
The magnetic field around 6 is changed. Therefore, the blade 16 rotates.
【００２０】この結果、回転羽根１６がシリンダ１１内
の懸濁液を攪拌する。よって、懸濁液の注出速度がきわ
めて遅い場合でも、上記羽根１６の回転により懸濁液を
攪拌するので、細胞やタンパク質などのような比重の大
きい粒子成分が沈降することが防止され、懸濁液の濃度
を均一に維持することができる。As a result, the rotary vanes 16 stir the suspension in the cylinder 11. Therefore, even if the pouring speed of the suspension is extremely slow, the suspension is agitated by the rotation of the blades 16, so that it is possible to prevent sedimentation of the particle components having a large specific gravity such as cells and proteins. The concentration of the suspension can be kept uniform.
【００２１】そして、上記実施例の場合、羽根１６を回
転駆動するための動力は、シリンダ１１の外部に設けた
磁石１８、１８によるから、シリンダ１１やプランジャ
１２を貫通して導入される部品が不要であり、いわゆる
非接触駆動であるためシリンダ１１やプランジャ１２に
格別なシール構造を設ける必要がなく、かつ懸濁液が漏
れ出ることがない。このため、リーク液による装置の汚
染や、周囲環境の汚染を防止することができる。特に、
人体が触れると危険性のある懸濁液や、細胞を取り扱う
注出装置の場合は、安全性が高くなる利点がある。な
お、本発明は上記実施例に制約されるものではない。In the case of the above embodiment, the power for driving the blades 16 to rotate is generated by the magnets 18, 18 provided outside the cylinder 11, so that the parts introduced through the cylinder 11 and the plunger 12 are introduced. Since it is unnecessary and so-called non-contact drive, it is not necessary to provide a special seal structure on the cylinder 11 or the plunger 12, and the suspension does not leak. Therefore, it is possible to prevent the device from being contaminated by the leak liquid and the surrounding environment. In particular,
In the case of a suspension, which is dangerous to the human body, or a pouring device that handles cells, there is an advantage that the safety is high. The present invention is not limited to the above embodiment.
【００２２】すなわち、上記実施例では、細胞およびタ
ンパク質などの懸濁液を取り扱う注出装置の場合を説明
したが、本発明はこれに限らず、シリンダ内で懸濁液が
沈殿して分離する可能性のある液体を取り扱う装置には
適用可能である。That is, in the above-mentioned embodiment, the case of the pouring device for handling the suspension of cells and proteins has been described, but the present invention is not limited to this, and the suspension is precipitated and separated in the cylinder. It is applicable to devices that handle potential liquids.
【００２３】また、羽根１６を回転駆動する構造は、シ
リンダの外部で磁石を回転駆動するように構成すること
に限らず、外部から導入した軸で羽根を回すようにして
もよい。Further, the structure for rotationally driving the blades 16 is not limited to the structure for rotationally driving the magnets outside the cylinder, and the blades may be rotated by an axis introduced from the outside.
【００２４】さらに、シリンダ内で懸濁液の沈降を防止
するには、注出器１０全体を振動させるなどの手段も考
えられるが、このような振動構造の場合は、駆動系との
連結構造が複雑になる心配があるので、好ましくはな
い。Further, in order to prevent the suspension from settling in the cylinder, it is conceivable to vibrate the entire pouring device 10 or the like. It is not preferable because it may be complicated.
【００２５】[0025]
【発明の効果】以上説明したように本発明によれば、シ
リンダ内に収容した羽根により懸濁液を攪拌するから、
粒子成分の沈降が防止され、懸濁液を均等な濃度に保つ
ことができる。As described above, according to the present invention, the suspension is stirred by the blades contained in the cylinder,
Settling of the particle components is prevented and the suspension can be kept at a uniform concentration.
【図１】本発明の一実施例を示す注出器の断面図。FIG. 1 is a sectional view of a dispenser showing an embodiment of the present invention.
【図２】図１のII−II線に沿う断面図。FIG. 2 is a sectional view taken along line II-II in FIG.
【図３】自動注出装置の全体の構造を示す構成図。FIG. 3 is a configuration diagram showing an overall structure of an automatic pouring device.
１…駆動モータ ２１〜２２…減速ギア列
３…クラッチ ４…従動軸 ５…送りねじ
６…送りナット ７…平歯車（駆動側） ８…平歯車（従動側） １０…注出器 １１…シリンダ
１２…プランジャ １３…注出ノズル １５…ステム
１６…羽根 １８…磁石1 ... Drive motors 21-22 ... Reduction gear train
3 ... Clutch 4 ... Driven shaft 5 ... Feed screw
6 ... Feed nut 7 ... Spur gear (drive side) 8 ... Spur gear (driven side) 10 ... Pourer 11 ... Cylinder
12 ... Plunger 13 ... Pouring nozzle 15 ... Stem
16 ... Feather 18 ... Magnet
Claims (1)
リンダおよびプランジャを用いて注出する装置におい
て、 上記シリンダ内に羽根を収容するとともに、この羽根を
外部から回転駆動する手段を有し、上記羽根の回転によ
り上記シリンダ内の懸濁液を攪拌することを特徴とする
沈降防止注出装置。1. A device for pouring out a suspension containing particle components having different specific gravities using a cylinder and a plunger, and having means for accommodating a vane in the cylinder and rotationally driving the vane from the outside. Then, the settling prevention pouring device characterized in that the suspension in the cylinder is agitated by the rotation of the blade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4284252A JPH06133758A (en) | 1992-10-22 | 1992-10-22 | Injection device preventing sedimentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4284252A JPH06133758A (en) | 1992-10-22 | 1992-10-22 | Injection device preventing sedimentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06133758A true JPH06133758A (en) | 1994-05-17 |
Family
ID=17676126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4284252A Withdrawn JPH06133758A (en) | 1992-10-22 | 1992-10-22 | Injection device preventing sedimentation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06133758A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007202449A (en) * | 2006-01-31 | 2007-08-16 | Fujitsu Ltd | Liquid-sending device |
| JP2008188535A (en) * | 2007-02-05 | 2008-08-21 | Toshiba Corp | Liquid discharge apparatus and liquid discharge method |
| JP2014004527A (en) * | 2012-06-25 | 2014-01-16 | Aitec:Kk | Fluid feeding apparatus |
| JP2014174134A (en) * | 2013-03-13 | 2014-09-22 | Kankyo Kagaku Corporation:Kk | Pretreatment method and pretreatment agent for turbid water sample related to radiation analysis; and pretreatment tool |
| WO2019177133A1 (en) * | 2018-03-15 | 2019-09-19 | テルモ株式会社 | Method for preparing cultured cells |
-
1992
- 1992-10-22 JP JP4284252A patent/JPH06133758A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007202449A (en) * | 2006-01-31 | 2007-08-16 | Fujitsu Ltd | Liquid-sending device |
| JP2008188535A (en) * | 2007-02-05 | 2008-08-21 | Toshiba Corp | Liquid discharge apparatus and liquid discharge method |
| JP2014004527A (en) * | 2012-06-25 | 2014-01-16 | Aitec:Kk | Fluid feeding apparatus |
| JP2014174134A (en) * | 2013-03-13 | 2014-09-22 | Kankyo Kagaku Corporation:Kk | Pretreatment method and pretreatment agent for turbid water sample related to radiation analysis; and pretreatment tool |
| WO2019177133A1 (en) * | 2018-03-15 | 2019-09-19 | テルモ株式会社 | Method for preparing cultured cells |
| JPWO2019177133A1 (en) * | 2018-03-15 | 2021-03-11 | テルモ株式会社 | Method for preparing cultured cells |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20000104 |