JPH0611093Y2 - Sample agitator for blood analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention relates to a sample stirring device of a fully automatic blood analyzer, and relates to a device for stirring a sample in a container that requires airtightness.

[Conventional technology]

Pretreatment such as blood dilution for automatic blood cell counting
In many cases, it is automated by controlling the pressure difference in the piping system with a valve. Therefore, the stirring of the sample just before measurement must be performed in a closed container.

As a conventional stirring method corresponding to that, the sample injection port 9 is directed toward a curved surface of the bottom surface such as the container body 1 of FIG.
Blood and diluent are vigorously injected from the container, and the inertial force causes a vortex to stir, or small bubbles are intermittently injected into the sample liquid from the bottom of the container body 1, The method of agitating by causing convection due to the buoyancy of the above has been put into practical use.

Further, in JP-A-60-125240, a cylindrical casing is provided vertically in the upper part of the stirring tank, a permanent magnet is arranged inside the cylindrical casing, and another permanent magnet is arranged opposite to the outside.
A leak-free stirrer in which a drive device is connected to one permanent magnet and the shaft of a stirring blade is supported by the other permanent magnet is described.

[Problems to be solved by the device]

However, the conventional method of stirring by the above vortex or convection has a problem that the stirring force is weak. In particular, when a mixed liquid such as a drug is added at the same time as stirring, there is a problem that measurement error occurs due to insufficient stirring.

Specifically, for example, when an erythrocyte hemolytic agent for counting white blood cells is injected from the medicine injection port 10 in FIG.
Due to insufficient agitation, uneven hemolysis of red blood cells occurs, resulting in an excessively large white blood cell count.

In addition, all of the conventional methods of stirring by vortex or convection have a problem that if the stirring is performed simultaneously with the measurement, an error will occur in the measured value.

It is also possible to put the magnet pieces in the sample container in advance and apply a rotating magnetic field from the outside to rotate the magnet pieces to stir them, but when replacing the sample, it is necessary to fix the magnet pieces to the bottom without fail. Since it remains and affects the measured value, it was difficult to put it into practical use.

The stirrer described in JP-A-60-125240 is suitable for use in the fields of fine chemical plants and bioindustries, but it has a problem in being used in the field of blood analysis.

The sample (diluted blood sample) and the drug (hemolytic agent) are injected into the inside of the container through the injection port in the upper part of the container, but they must be quickly mixed. In this case, if the shape of the container is irregular (internal diameter is not constant), in order to quickly and uniformly mix the two liquids injected from above, as shown in FIG. When it is installed diagonally, the liquid also rotates in the vertical direction, which is convenient for good stirring, but like the stirrer described in JP-A-60-125240, the stirring shaft is vertical to the container. If it is attached to (see Fig. 4), quick and uniform mixing is not possible.

The present invention has been made in view of these problems, and it is possible to perform sufficient agitation in a short time, agitation during measurement is possible, and a small amount of sample remains during sample exchange. It is intended to provide a stirring device.

[Means for Solving the Problems] In order to achieve the above object, the sample agitator for a blood analyzer of the present invention is provided with a blood sample, which will be described with reference to FIGS. 1 and 2. A blood analyzer provided with a closed container formed of a container body 1 and a lid 4, and a blood cell detector 7 and an electrode 8 arranged in the closed container, wherein an outer magnet plate provided outside the closed container 20, a motor 14 for rotating the outer magnet plate 20, an agitating rod holding part 6 having a cover 18 made of a non-magnetic material at the upper end, which is obliquely and airtightly attached to the closed container, and this agitating device. A stirring rod 15 which is rotatably held by the rod holding unit 6, has an inner magnet plate 16 at a position adjacent to the outer magnet plate 20 at the upper end, and has a blade 22 for stirring a sample at the lower end, and a cover 18. Of the frictional resistance provided between the magnet plate 16 and It is characterized in that it comprises a sheet 17 made again material.

Usually, the cover 18 is made of synthetic resin or the like, and the sheet 17 is made of Teflon or the like. However, the cover 17 is not limited to these, and other materials can be used.

[Action]

When the outer magnet plate 20 rotates due to the rotation of the motor 14, the inner magnet plate 16 that faces the cover 18 that is a non-magnetic body and the sheet 17 that is made of a material having a small friction resistance faces the rotation shaft and rotates. The sheet 17 prevents the inner magnet plate 16 from being rubbed against the cover 18 and worn.

The stirring rod 15 and the blade 22 are rotated by the rotation of the inner magnet plate 16, and the blade 22 in the sample liquid causes the liquid to flow and agitates.

Further, since the inside of the container is kept airtight, the sample in the container body 1 can be exchanged by the pressure control means from the outside.

〔Example〕

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the drawings. However, unless otherwise specified, the materials, shapes, relative positions, etc. of the constituent devices described in this embodiment are not intended to limit the scope of the present invention to them only, and are merely descriptions. It's just an example.

FIG. 1 shows an embodiment of the device of the present invention. The container body 1 is fixed to the lid 4 by the O-ring 2 and the flange 3, and the lid 4 is fixed to the support body 5 in contact with the upper surface.

Further, the stirring rod holding unit 6, the detector 7, the sample injection port 9,
The drug injection port 10 penetrates the lid 4 and the support 5 and is attached to the lid 4 while keeping the container body 1 airtight.

The stirring rod holding portion 6 is obliquely attached to the lid 4 with an O-ring 11 and a screw 12, and the supporting member 5
Similarly, a screw is fixed to a frame 13 attached to the motor in parallel with the motor 14.

At the center of the stirring rod holding portion 6, a stirring rod 15 having blades 22 at the lower end for allowing liquid to flow is rotatably inserted, and an inner magnet plate 16 is attached to the upper end of the stirring rod 15. . Small magnets are embedded on the surfaces of the inner magnet plate 16 and the outer magnet plate 20 as shown in FIG.

A Teflon sheet 17 is placed on the upper surface of the inner magnet plate 16, and the outside thereof is covered with a cover 18 made of synthetic resin.

The cover 18 keeps the stirring rod holder 6 airtight.
The ring 19 is sandwiched and screwed. The motor 1
An outer magnet plate 20 is attached to the rotating shaft of No. 4 so as to face the inner magnet plate 16. Reference numeral 23 is an outlet, and 24 is a detector pellet.

In the apparatus of the embodiment having the above configuration, first, the container body 1
The sample 21 is placed inside the sample injection port 9 by controlling the pressure from the outside.
, Then the motor 14 is activated and the outer magnet plate 20 rotates. Since the magnetic force of the outer magnet plate 20 reaches the inner magnet plate 16, the inner magnet plate 16 also rotates overcoming the frictional resistance of the Teflon sheet 17. The rotational force of the inner magnet plate 16 is transmitted to the blade 22 in the sample solution through the stirring rod 15, and the blade 22 stirs the sample.

In addition, in this embodiment, prior to stirring, the drug injection port 1
It is also possible to add a drug from 0 and to perform particle detection by the electrode 8 and the detector 7 at the same time as stirring.

[Effect of device]

Since the sample stirring device for the blood analyzer of the present invention is configured as described above, it has the following effects.

(1) The measurement sample in the closed container just before the measurement is sufficiently stirred, and the measurement accuracy is improved. In particular, the non-uniformity of hemolysis due to poor stirring of red blood cell hemolytic agents is eliminated,
The accuracy of measurement of white blood cell count value and white blood cell particle size distribution is improved.

(2) In addition to stirring during measurement, it is possible to exchange samples and inject drugs by pressure control.

(3) The stirring bar is attached diagonally to the container,
As shown in FIG. 3, since the liquid also rotates in the vertical direction, even if the shape of the container is irregular, the liquid can be quickly and uniformly mixed.

[Brief description of drawings]

FIG. 1 is a partial cross-sectional explanatory view showing an embodiment of a sample stirring device for a blood analyzer of the present invention, and FIG. 2 is a plan explanatory view showing states of magnetic poles of an inner magnet plate and an outer magnet plate in FIG. , Third
FIG. 4 is an explanatory view showing a stirring state in the apparatus of the present invention, and FIG. 4 is an explanatory view showing a stirring state when the stirring shaft is vertically attached to the container. 1 ... Container body, 2, 11, 19 ... O-ring, 3 ...
Flange, 4 ... Lid, 5 ... Support, 6 ... Stirring rod holder, 7 ... Detector, 8 ... Electrode, 9 ... Sample inlet, 1
0 ... Medicine injection port, 12 ... Screw, 13 ... Cradle, 14
...... Motor, 15 …… Stirring rod, 16 …… Inner magnet plate, 17
...... Sheet, 18 ...... Cover, 20 ...... Outer magnet plate, 21
...... Sample, 22 …… Wing, 23 …… Discharge port, 24 …… Detector pellet

Claims (1)

[Scope of utility model registration request] 1. A closed container formed by a container body (1) for containing a blood sample and a lid (4), a blood cell detector (7) and an electrode (8) arranged in the closed container. In the blood analyzer, the outer magnet plate (20) provided outside the closed container, the motor (14) for rotating the outer magnet plate (20), and the airtight and diagonally attached to the closed container. And a stirring rod holding portion (6) having a cover (18) made of a non-magnetic material at the upper end, and rotatably held by the stirring rod holding portion (6), and the outer magnet plate (20) at the upper end. The inner magnet plate (1
6) and a stirring rod (15) having a blade (22) at the lower end for stirring the sample, and a material having a small frictional resistance provided between the cover (18) and the inner magnet plate (16). A sample stirring device for a blood analyzer, comprising: a sheet (17).