JPH0610310Y2 - Diffusion limiting membrane holder - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial field of application> The present invention relates to a diffusion limiting film holder, and more specifically, to store a diffusion limiting film that is brought into close contact with the surface of a concentration measuring electrode until use. The present invention relates to a diffusion limiting membrane holder that is preferably used.

<Prior Art> Conventionally, a physiologically active substance is immobilized on the surface of an electrode, paying attention to the characteristic that it can detect extremely complicated organic compounds, proteins, etc. with extremely high sensitivity and selectively. Research and development have been conducted to measure the above-mentioned organic compounds, proteins and the like by means of enzyme electrodes.

When the target substance is measured using the enzyme electrode, normally, the target substance to be measured is oxidized or reduced in the presence of the physiologically active substance, and the amount of the produced substance or the lost substance is measured. Since the concentration of the substance to be measured is measured by the measurement, the upper limit of the measurable concentration is limited by the existing amount of the substance that causes oxidation, reduction, etc., such as oxygen.

Focusing on such a point, an attempt has been made to increase the measurement limit concentration by attaching a diffusion limiting membrane on the surface side of the immobilized enzyme membrane on which the physiologically active substance is immobilized and limiting the permeation rate of the substance to be measured. .

Specifically, a configuration is adopted in which a diffusion limiting film is attached to a cap screwed to a rod-shaped enzyme electrode base, and the diffusion limiting film is automatically attached to the immobilized enzyme film by screwing the cap. There is.

Further, by adopting such a configuration, the diffusion limiting film limits the permeation rate of the substance to be measured and enables measurement up to a considerably high concentration. Further, the diffusion limiting film is removable in order to eliminate the influence of the interfering substance contained in the solution to be measured (for example, increase in the diffusion limiting effect due to the adhesion of the interfering substance) and perform good measurement. The screwing mechanism by the cap is adopted.

<Problems to be Solved by the Invention> When the diffusion limiting membrane holder having the above configuration is adopted, the diffusion limiting membrane can be exchanged relatively easily. There is a problem that attachment and detachment becomes very difficult depending on the situation, and it becomes impossible to achieve a uniform adherence state to the immobilized enzyme membrane depending on the amount of force applied during screwing.

In addition, when the enzyme electrode base has a small diameter and, as a result, the cap becomes small, the manual operation becomes very difficult, and the above problem becomes more remarkable.

Further, since the diffusion limiting film is attached to the cap, there is a problem that the space required for storage and transportation becomes large.

In consideration of such problems, a diffusion limiting film holder having a configuration in which an opening is formed at a predetermined position of a thin plate having a predetermined shape and a diffusion limiting film is attached so as to cover one side of the opening is adopted. It is possible to measure the concentration of the test solution while the restriction membrane is in pressure contact with the immobilized enzyme membrane.By adopting such a configuration, the diffusion restriction membrane can be easily replaced every time the measurement of the test solution is performed. However, the influence of interfering substances can be eliminated and accurate concentration measurement can be performed.

However, when the test solution is a body fluid typified by blood, it is generally necessary to collect the body fluid and drop it on the diffusion limiting film, and then wipe off the excess body fluid. The extra body fluid is wiped off by.

As a result, every time body fluid is measured, excess tissue fluid must be wiped away, and the tissue paper must be discarded.If the thin plates with the diffusion limiting film are individually packaged, the packaging bag must be removed. Also has to be disposed of, tissue paper and the like must be prepared in advance, and there is a problem that the amount of dust generated during body fluid measurement increases.

<Purpose of Invention> This invention was made in view of the above problems.
It is an object of the present invention to provide a diffusion limiting film holder that does not require preparation of tissue paper or the like in advance and can reduce the amount of dust generated during body fluid measurement.

<Means for Solving Problems> In order to achieve the above-mentioned object, the diffusion limiting film holder of the present invention has an opening for passing a test solution at a predetermined position of a thin plate having a predetermined shape. A diffusion limiting film is attached to one side surface of the thin plate so as to cover the opening, and the bag containing the thin plate has a film capable of impregnating the test solution at least in part.

However, while the bag is composed of a sealable thin plate material as a whole and may have a film on a part of the inner surface of the thin plate material, it may have a film on the entire inner surface of the thin plate material. Good. The membrane in these cases is preferably one that has been subjected to a hydrophilization treatment, such as filter paper, absorbent paper, or a degreased natural fiber, a hydrophilized organic polymer fiber, and glass. It is possible to use a film or the like formed in a cloth shape or a non-woven cloth shape using fibers, carbon fibers or the like.

<Operation> With the diffusion limiting film holder having the above structure, an opening for passing a test solution is formed at a predetermined position of a thin plate having a predetermined shape, and diffusion limiting is performed on one side surface of the thin plate so as to cover the opening. Since the membrane is attached and the bag containing the thin plate has a membrane that can be impregnated with the test solution at least in part, open the bag when measuring the test solution. The thin plate to which the diffusion limiting film is attached is taken out, the test solution is dropped in the opening portion, and then the thin plate is attached to the concentration measuring device to measure the concentration of the test solution.

Excess test solution remaining on the fingers etc. can be wiped off with a film that can be impregnated with the test solution, and then the bag can be discarded, so there is no need to prepare tissue paper in advance. In addition, the amount of dust generated each time the test solution is measured can be suppressed to a necessary minimum.

In particular, if the thin plate on which the test solution has been dropped is placed in a bag and discarded, it is possible to reliably prevent the diffusion limiting film after the measurement of the test solution and the contamination and infection caused by the test solution remaining on the thin plate. You can

When the bag is composed of a thin sheet material that can be sealed as a whole and has a film on a part of the inner surface of the thin sheet material, the strength of the entire bag is ensured by the thin sheet material, and diffusion is performed. The storage state of the thin plate to which the limiting film is attached can be favorably maintained. In addition, when the thin plate member has a film on the entire inner surface, the wiping amount of the test solution can be increased.

<Example> Hereinafter, detailed description will be given with reference to the accompanying drawings illustrating an example.

FIG. 1 is a partially cutaway perspective view showing an embodiment of the diffusion limiting film holder of the present invention, and FIG. 2 is a longitudinal sectional view showing a resistance to a test solution and a certain degree of flexibility. A circular opening (2) is formed at a position separated by a predetermined distance from one short side of the substantially rectangular thin plate (1) having flexibility, and the opening (2) is formed on the lower surface of the thin plate (1). ) So that it covers the diffusion limiting film
(3) is attached by adhesion or the like. The thin plate (1) to which the diffusion limiting film (3) is attached is housed in the sealed bag (4), and the inner surface of the bag (4) is made to directly face the diffusion limiting film (3) and covered. A blotter paper (5) that can be impregnated with the test solution is attached. In addition, a positioning hole is provided at a predetermined position of the thin plate (1).
(6) is formed.

Fig. 3 is a plan view showing the main part of the blood glucose level measuring device, and Fig. 4 is a longitudinal sectional view. An enzyme electrode (8) is attached at a predetermined position on the base so that the electrode surface projects upward. At the same time, it has a through hole (10) through which the enzyme electrode (8) can be inserted, and has a housing groove (11) for housing the diffusion limiting film holder at a predetermined position of the diffusion limiting film holder mounting member (9). Engaging portions (12) and (13) are provided at opposing positions across the through hole (10), that is, at the innermost position and at a predetermined position near the side where the diffusion limiting film holder is inserted and removed. More specifically, the engagement portion (12) has an engagement recess (12a) whose inner depth is formed in an arcuate surface having the same radius of curvature as one short side of the thin plate (1). In addition, it has a ridge (12b) that constitutes an upper regulating protrusion of the engagement recess (12a).

The ridge (12b) may have a wide width over the entire width of the engaging recess (12a), or may be narrower than the entire width of the engaging recess (12a). Good. Further, the engaging portion (13) is an upright portion having a plane shape substantially equal to the plane shape of the positioning hole (6). The surface of the enzyme electrode (8) is formed into a substantially arcuate surface.

When dripping blood (14) onto the diffusion limiting membrane holder having the above structure, the bag (4) is opened, the thin plate (1) is taken out, and the opening (2) is placed on any surface. The blood (14) may be dropped in the vicinity of (), and the dropped blood (14) is guided to the diffusion limiting film (3).

The excess blood remaining on the fingers etc. is removed from the bag (4) above.
Since it can be wiped off by the blotting paper (5) attached to the inside of the, it is not necessary to prepare tissue paper in advance, and the generated dust is also in the bag.
Only (4) can reduce the amount of garbage.

When the diffusion limiting film is attached to the blood glucose level measuring device having the above-mentioned configuration, one of the short sides (6) of the thin plate (1) is engaged with the diffusion limiting film (3) on the lower surface. The thin plate (1) is housed in the housing groove (11) so that the diffusion limiting film (3) faces the through hole (10) (see FIG. 5A).
Then, by moving the diffusion limiting film holder attachment member (9) downward, the surface of the enzyme electrode (8) relatively penetrates the through hole (10).
It can stick out to the diffusion limiting film (3) attached to the thin plate (1) (see FIG. 5B).

Therefore, in this state, blood (14) is
It reaches the surface of the enzyme electrode (8) through (3),
The diffusion limiting membrane (3) limits the diffusion of glucose, and since it is guided to the enzyme electrode (8) in a state where the concentration is low to some extent, even if the glucose concentration in the blood is high, the diffusion is limited and considerably. The glucose concentration can be measured based on the lowered concentration.

Further, after the measurement is completed, the diffusion limiting film (3) can be easily removed by operating in the reverse of the above-mentioned mounting. Thereafter, the thin plate (1) to which the diffusion limiting film (3) is attached is stored in the opened bag (4) and discarded, whereby the diffusion limiting film (3) and the blood remaining on the thin plate (1) are discarded. Pollution caused by,
It is possible to reliably prevent infection and the like.

As is clear from the above description, the blotting paper (5) is attached to the inner surface of the bag (4), and since the blotting paper (5) can be made considerably thin, the packaging state Even if a large number of the diffusion limiting films (3) in (1) are laminated, the bulkiness can be reduced, and storage and transportation can be reliably performed in a small space.

Incidentally, the diffusion limiting film (3) is one that has a high diffusion limiting effect on glucose while blocking the permeation of large particles such as blood cells in blood (for example, cellophane having a predetermined count).
The diffusion limiting membrane having a high diffusion limiting effect on glucose is attached to the enzyme electrode (8) in advance, and the diffusion limiting membrane (3) attached to the thin plate (1) is used as a diffusion limiting membrane for glucose. It is possible to use a material (for example, a polycarbonate membrane having a predetermined pore size, pore density, etc.) that has a relatively low effect and can reliably block the passage of large particles such as blood cells. Can keep the diffusion limiting effect on glucose as a whole of both diffusion limiting membranes almost constant, and as a result,
Despite the need to replace the diffusion limiting membrane (3) clogged with large particles, the blood glucose level measurement accuracy can be improved.

<Embodiment 2> FIG. 6 is a partially cutaway perspective view showing another embodiment. The only difference from the above embodiment is that the blotting paper (5) is attached to the entire inner surface of the bag (4). Yes, the other parts have the same configuration.

Therefore, in the case of this embodiment, the amount of blood that can be wiped off by the blotting paper (5) can be significantly increased, and excess blood can be reliably wiped off even if the amount of blood collected from the finger or the like is large.

After the blood glucose measurement operation is completed, the thin plate (1)
It is possible to surely absorb excess blood remaining in the diffusion limiting membrane (3) and the thin plate (1) and certainly prevent contamination, infection, etc. it can.

Incidentally, the present invention is not limited to the above-mentioned embodiment, for example, the bag (4) can be constituted by the membrane (5) capable of impregnating blood, and the outer surface of the bag (4) can be formed. Membrane (5) can be attached to at least a part, and it can be applied to a diffusion limiting membrane holder for measuring the concentration of a specific substance contained in any test solution other than blood. Besides, various design changes can be made within the scope of the invention.

<Effect of Device> As described above, in the first device, the excess test solution remaining on the finger or the like can be wiped off with a film that can be impregnated with the test solution, and then the bag can be discarded. Not only is it unnecessary to prepare tissue paper or the like in advance, but it also has the peculiar practical effect that the amount of dust generated each time the test solution is measured can be suppressed to a necessary minimum.

In particular, if the thin plate on which the test solution has been dropped is placed in a bag and discarded, it is possible to reliably prevent the diffusion limiting film after the measurement of the test solution and the contamination and infection caused by the test solution remaining on the thin plate. You can

In the second invention, the strength of the bag as a whole is secured by the thin plate material, and the storage state of the thin plate to which the diffusion limiting film is attached can be favorably maintained.

[Brief description of drawings]

FIG. 1 is a partially cutaway perspective view showing an embodiment of the diffusion limiting membrane holder of the present invention, FIG. 2 is a longitudinal sectional view, FIG. 3 is a plan view showing the main part of a blood glucose level measuring device, and FIG. FIG. 5 is a longitudinal sectional view, FIG. 5 is a view for explaining a mounting operation, and FIG. 6 is a partially cutaway perspective view showing another embodiment. (1) …… thin plate, (2) …… opening, (3) …… diffusion limiting film, (4) …… bag, (5) …… blotting paper

Claims (2)

[Scope of utility model registration request] 1. An opening (2) for passing a test solution is formed at a predetermined position of a thin plate (1) having a predetermined shape, and diffused on one side surface of the thin plate (1) so as to cover the opening (2). The limiting membrane (3) is attached, and further, the bag (4) for accommodating the thin plate (1) is characterized by having a membrane (5) capable of impregnating a test solution at least in part. A diffusion limiting membrane holder. 2. The bag (4) is composed of a sealable thin plate material as a whole, and a film (5) is formed on a part of the inner surface of the thin plate material.
The diffusion limiting film holder according to claim 1, which has the above-mentioned utility model registration claim.