JPH058677B2 - - Google Patents
Info
- Publication number
- JPH058677B2 JPH058677B2 JP62118881A JP11888187A JPH058677B2 JP H058677 B2 JPH058677 B2 JP H058677B2 JP 62118881 A JP62118881 A JP 62118881A JP 11888187 A JP11888187 A JP 11888187A JP H058677 B2 JPH058677 B2 JP H058677B2
- Authority
- JP
- Japan
- Prior art keywords
- linolenic acid
- lipid
- carbon source
- mucor
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000002632 lipids Chemical class 0.000 claims description 35
- VZCCETWTMQHEPK-UHFFFAOYSA-N gamma-Linolensaeure Natural products CCCCCC=CCC=CCC=CCCCCC(O)=O VZCCETWTMQHEPK-UHFFFAOYSA-N 0.000 claims description 30
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 claims description 30
- 235000020664 gamma-linolenic acid Nutrition 0.000 claims description 30
- 229960002733 gamolenic acid Drugs 0.000 claims description 30
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 16
- 229910052799 carbon Inorganic materials 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 241000306281 Mucor ambiguus Species 0.000 claims description 8
- 235000000346 sugar Nutrition 0.000 claims description 5
- 241000498617 Mucor javanicus Species 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 claims 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 claims 1
- 229960004488 linolenic acid Drugs 0.000 claims 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 claims 1
- 244000005700 microbiome Species 0.000 description 18
- 230000001580 bacterial effect Effects 0.000 description 14
- 239000000203 mixture Substances 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000235395 Mucor Species 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- 241000134363 Umbelopsis ramanniana Species 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 241000235388 Mucorales Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 150000001879 copper Chemical class 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- HOBAELRKJCKHQD-QNEBEIHSSA-N dihomo-γ-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCCCC(O)=O HOBAELRKJCKHQD-QNEBEIHSSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229940094443 oxytocics prostaglandins Drugs 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
［産業上の利用分野］
本発明はγ−リノレン酸含有脂質の製造法に関
し、詳しくは特定の微生物を用いてγ−リノレン
酸含量の高い脂質を効率よく製造する方法に関す
る。
［従来の技術、発明が解決しようとする問題点］
微生物によるγ−リノレン酸含有脂質の生産に
ついては、これまでにいくつかの報告がある
（R.O.Mumma：Lipids、６、584（1971）、R.
Shaw：Biochem.Biophys.Acta.98、230（1965）、
鈴木ら：油化学、30、863（1981）、特開昭61−
37097）。しかし、これら従来法では生産される全
脂質中のγ−リノレン酸含量は低く、精々20％で
ある。
γ−リノレン酸およびこれから生体内で合成さ
れるビスホモ−γ−リノレン酸およびアラキドン
酸はプロスタグランジンの前駆体であることか
ら、食品、医薬品等の分野での利用が期待され、
γ−リノレン酸を大量かつ安定的に得る方法が渇
望されている。
［問題点を解決するための手段］
そこで本発明者らは、γ−リノレン酸を30％以
上含有する脂質を生産する能力を有する脂質を生
産する能力を有する微生物の検索を行い、ムコー
ル属に属する微生物が該能力を有していることを
見出し、本発明を完成するに至つた。
すなわち本発明は、ムコール属に属し、γ−リ
ノレン酸を30％以上含言有する脂質生産能を有す
る微生物」を「ムコール・シルシネロイデス
（Mucor circinelloides）またはムコール・ジヤ
バニクス（Mucor javanicus）」を炭素源として
糖類または酢酸ソーダを含む培地に培養し、培養
物から「γ−リノレン酸を30％以上含有する脂
質」を採取することを特徴とするγ−リノレン酸
含有脂質の製造法並びに該微生物の培養中に培地
に前記炭素源を追加して培養を行い、培養物から
γ−リノレン酸含有脂質を採取することを特徴と
するγ−リノレン酸含有脂質の製造法を提供する
ものである。
本発明に使用する微生物は、前述の如くγ−リ
ノレン酸を30％以上含有する脂質を生産しうるも
のであることを第１の条件とし、さらに該脂質含
量が乾燥菌体重量の25％以上であることを目標と
して、接合菌類を中心にスクリーニングして得ら
れたものである。この微生物は広島大学工学部醗
酵工学講座所有のものであり、栄養細胞は菌糸状
で隔壁を欠き、また胞子のう内に胞子のう胞子を
作り、無性生殖を行うなどムコール属に特有の菌
学的性質を有している。
このムコール属微生物についてH.Zycha、R.
Siepmann、G.Linnemann著、「Mucorales」
（1969）（J.Cramer発行）により同定を行つたと
ころ、菌株（HUT1121株）はムコール・シル
シネロイデス（Mucor circinelloides）であり、
菌株（HUT 1162株）はムコール・ジヤバニ
クス（M.javanicus）であることが判つた。これ
ら菌株、は微生物工業技術研究所にそれぞれ
FERM Ｐ−9359、FERM Ｐ−9360として寄託
されている。
本発明には上記微生物のほか、これらから誘導
される変異株であつて上記のようにγ−リンレン
酸高含有脂質を生産する能力を有するもの等も等
しく使用することができる。
上記微生物を培養するための倍地は、該微生物
が良く生育して目的とする脂質を生産しうるもの
であればよく、炭素源、窒素源、無機塩類および
必要により微生物の生育に好適なアミノ酸等の成
分を含むものが用いられる。炭素源としてはグル
コース、でんぷん、廃糖蜜等の糖類や酢酸ソーダ
などが使用でき、特にグルコース等の糖類が好適
である。また、窒素源としてはアンモニウム塩、
酵母エキス、コーン、ステイーブ、リカー、ペプ
トンなどがあり、無機塩類としてはマグネシウム
塩、カルシウム塩、リン酸塩、鉄塩、銅塩などが
ある。
培養にあたり、炭素源は倍地に最初から全量を
加えてもよいが、培養開始後適当な時期に追加す
ることによつてγ−リノレン酸の生産量を増大さ
せることができる。グルコース等の炭素源を倍地
に最初から加える場合、初発添加量が多過ぎる
と、微生物の生育に悪影響を及ぼすことがあるの
で、通常は50〜250g／、好ましくは100〜
200g／とすべきである。また、炭素源を培養
中に追加する場合、その時期、回数などは適宜決
定すればよい。たとえば、グルコース等の炭素源
の初発濃度を100〜200g／として培養を行い、
炭素源の大部分が消費されたときに50〜100g／
程度の炭素源を追加（１回もしくは数回に分け
て）することにより菌体の増殖を高め、結果的に
γ−リノレン酸高含量の脂質の生産量を増すこと
ができる。
その他の培養条件、たとえば温度、時間帯は使
用する微生物の性質等を考えて目的とする脂質の
生産量が高くなるような条件を設定すればよい。
通常は20〜32℃、好ましくは25〜30℃、PH３〜
７、好ましくは3.5〜６にて60〜120時間、好まし
くは70〜100時間行えばよい。
γ−リノレン酸を含有する脂質は通常、微生物
菌体中に蓄積されるので、常法により培養液を
固・液分離し、該脂質を含む菌体を得る。γ−リ
ノレン酸の用途によつては該菌体自体をそのまま
用いることも可能であるが、さらに精製するため
には、該菌体からBligh ＆ Dyer法、Folich法
などの抽出操作により目的とするγ−リノレン酸
含有脂質を得ることができる。
［実施例］
次に、本発明を実施例により詳しく説明する。
実施例 １
第１表に示した組成の倍地（PH４）６を10
容ジヤーフアーメンターに入れ、この倍地にムコ
ール・シルシネロイデスHUT 1121（FERM Ｐ
−9359）を接種し、30℃で３日間通気撹拌培養を
行つた。
第１表
グルコース 100g／
硫酸アンモニウム 6.6 〃
KH2PO4 4.5〃
MgSO4・7H2O 0.5 〃
酵母エキス 0.3 〃
FeSO4・7H2O 20mg／
CaCl2・2H2O 2.4 〃
CuSO4・5H2O 0.4 〃
ZnSO4・7H2O 2.0 〃
MnCl2・4H2O 2.0 〃
培養終了後、培養液を過して菌体を回収し、
乾燥菌体として21.6g／の菌体を得た。この菌
体をBligh ＆ Dyer法により抽出し、γ−リノ
レン酸含量32.4％の脂質6.4g／を得た。得られ
た脂質の脂質酸組成を第２表に示す。
第２表脂肪酸
含量（％）
16：０ 12.3
18：０ 1.7
18：１ 27.6
18：２ 16.0
18：3* 32.4 ＊γ−リノレン酸
実施例 ２
第３表に示した組成の倍他（PH5.5）18を30
容ジヤーフアーメンターに入れ、この倍地にム
コール・シルシネロイデスHUT 1121（FERM
Ｐ−9359）を接種し、30℃で４日間通気撹拌培養
を行つた。
第３表
グルコース* 150g／＋100g／
硫酸アンモニウム 19.8g／
KH2PO4 9.0 〃
MgSO4・7H2O 1.0 〃
酵母エキス 0.6 〃
FeSO4・7H2O 40mg／
CaCl2・2H2O 4.8 〃
CuSO4・5H2O 0.8 〃
ZnSO4・7H2O 4.0 〃
MnCl2・4H2O 4.0 〃
＊初発濃度150g／で培養を開始し、45時間
後に100g／を追加した。
培養終了後、実施例１と同様に処理して乾燥菌
体として58.01／の菌体を得た。この菌体から
実施例１と同様に抽出処理してγ−リノレン酸
34.4％を含む脂質14.7g／を得た。得られた脂
質の脂肪酸組成を第４表に示す。
第４表脂肪酸
含量（％）
16：０ 10.6
18：０ 2.3
18：１ 27.4
18：２ 18.1
18：３ 34.4
実施例 ３
実施例２において、ムコール・シルシネロイデ
スHUT 1121（FERM Ｐ−9359）に代りに第５
表に示した微生物を用いたこと以外は実施例２と
同様に行つた。菌体の収量、γ−リノレン酸含量
および脂質収量を第５表に示す。
[Industrial Application Field] The present invention relates to a method for producing a γ-linolenic acid-containing lipid, and more particularly to a method for efficiently producing a lipid with a high γ-linolenic acid content using a specific microorganism. [Prior art and problems to be solved by the invention] There have been several reports on the production of γ-linolenic acid-containing lipids by microorganisms (ROMumma: Lipids, 6 , 584 (1971), R.
Shaw: Biochem.Biophys.Acta. 98 , 230 (1965),
Suzuki et al.: Oil Chemistry, 30 , 863 (1981), JP-A-61-
37097). However, in these conventional methods, the content of γ-linolenic acid in the total lipid produced is low, at most 20%. γ-Linolenic acid and bishomo-γ-linolenic acid and arachidonic acid, which are synthesized from it in vivo, are precursors of prostaglandins, so they are expected to be used in fields such as food and medicine.
There is a need for a method for stably obtaining γ-linolenic acid in large amounts. [Means for Solving the Problems] Therefore, the present inventors conducted a search for microorganisms that have the ability to produce lipids that have the ability to produce lipids containing 30% or more of γ-linolenic acid, and found that they belong to the genus Mucor. The inventors discovered that the microorganisms to which they belong have this ability, and have completed the present invention. That is, the present invention uses "a microorganism belonging to the genus Mucor and having a lipid-producing ability containing 30% or more of γ-linolenic acid" using "Mucor circinelloides or Mucor javanicus" as a carbon source. A method for producing a γ-linolenic acid-containing lipid, which is characterized by culturing in a medium containing sugars or sodium acetate, and collecting a “lipid containing 30% or more γ-linolenic acid” from the culture, and during cultivation of the microorganism. The present invention provides a method for producing a γ-linolenic acid-containing lipid, which comprises adding the above-mentioned carbon source to a medium, culturing, and collecting the γ-linolenic acid-containing lipid from the culture. The first condition for the microorganism used in the present invention is that it is capable of producing a lipid containing 30% or more of γ-linolenic acid as described above, and furthermore, the lipid content is 25% or more of the dry bacterial weight. This was obtained by screening mainly Zygomycetes with the goal of This microorganism is owned by the Department of Fermentation Engineering, Faculty of Engineering, Hiroshima University, and its vegetative cells are hyphae-like and lack septation walls, and it produces sporangium within sporangia and reproduces asexually. It has certain characteristics. About this Mucor microorganism, H.Zycha, R.
Siepmann, G. Linnemann, "Mucorales"
(1969) (published by J. Cramer), the strain (HUT1121 strain) was identified as Mucor circinelloides.
The bacterial strain (HUT strain 1162) was found to be M. javanicus. These strains were sent to the Microbial Technology Research Institute.
Deposited as FERM P-9359 and FERM P-9360. In addition to the above-mentioned microorganisms, mutant strains derived from these microorganisms and having the ability to produce lipids with a high content of γ-phosphoric acid as described above can be equally used in the present invention. The medium for culturing the above-mentioned microorganisms may be any medium that allows the microorganisms to grow well and produce the desired lipid, and contains a carbon source, a nitrogen source, inorganic salts, and, if necessary, amino acids suitable for the growth of the microorganisms. Those containing the following ingredients are used. As the carbon source, sugars such as glucose, starch, blackstrap molasses, and sodium acetate can be used, and sugars such as glucose are particularly preferred. In addition, as a nitrogen source, ammonium salt,
Examples include yeast extract, corn, stave, liquor, and peptone, and inorganic salts include magnesium salts, calcium salts, phosphates, iron salts, and copper salts. During culture, the entire amount of the carbon source may be added to the medium from the beginning, but the production amount of γ-linolenic acid can be increased by adding it at an appropriate time after the start of culture. When adding carbon sources such as glucose to the medium from the beginning, if the initial addition amount is too large, it may have a negative effect on the growth of microorganisms, so it is usually 50 to 250 g/, preferably 100 to
It should be 200g/. Furthermore, when adding a carbon source during culture, the timing, number of times, etc. may be determined as appropriate. For example, culture is performed with an initial concentration of carbon source such as glucose at 100 to 200 g/
50-100g/ when most of the carbon source is consumed
By adding a certain amount of carbon source (in one time or in several parts), the growth of bacterial cells can be increased, and as a result, the production amount of lipids with a high content of γ-linolenic acid can be increased. Other culture conditions, such as temperature and time, may be set so as to increase the production of the desired lipid, taking into consideration the properties of the microorganisms used.
Usually 20~32℃, preferably 25~30℃, PH3~
7, preferably 3.5 to 6 for 60 to 120 hours, preferably 70 to 100 hours. Since lipids containing γ-linolenic acid are usually accumulated in microbial cells, the culture solution is separated into solid and liquid by a conventional method to obtain microbial cells containing the lipid. Depending on the use of γ-linolenic acid, it is possible to use the bacterial cells themselves as they are, but for further purification, the desired product can be extracted from the bacterial cells by an extraction procedure such as the Bligh & Dyer method or the Folich method. A γ-linolenic acid-containing lipid can be obtained. [Example] Next, the present invention will be explained in detail with reference to Examples. Example 1 Mixture (PH4) 6 to 10 with the composition shown in Table 1
Add Mucor circineroides HUT 1121 (FERM P
-9359) was inoculated and cultured with aeration at 30°C for 3 days. Table 1 Glucose 100g / Ammonium sulfate 6.6 〃 KH 2 PO 4 4.5〃 MgSO 4・7H 2 O 0.5 〃 Yeast extract 0.3 〃 FeSO 4・7H 2 O 20mg / CaCl 2・2H 2 O 2.4 〃 CuSO 4・5H 2 O 0.4 〃 ZnSO 4・7H 2 O 2.0 〃 MnCl 2・4H 2 O 2.0 〃 After culturing, strain the culture solution and collect the bacterial cells.
21.6 g of dried bacterial cells were obtained. This bacterial cell was extracted by the Bligh & Dyer method to obtain 6.4 g of lipid with a γ-linolenic acid content of 32.4%. The lipid acid composition of the obtained lipid is shown in Table 2. Table 2 Fatty acid content (%) 16:0 12.3 18:0 1.7 18:1 27.6 18:2 16.0 18:3 * 32.4 *γ-Linolenic acid Example 2 Double the composition shown in Table 3 (PH5. 5) 18 to 30
Add Mucor circinelloides HUT 1121 (FERM) to this double base.
P-9359) was inoculated and cultured with aeration and stirring at 30°C for 4 days. Table 3 Glucose * 150g/+100g/ Ammonium sulfate 19.8g/ KH 2 PO 4 9.0 〃 MgSO 4・7H 2 O 1.0 〃 Yeast extract 0.6 〃 FeSO 4・7H 2 O 40mg/ CaCl 2・2H 2 O 4.8 〃 CuSO 4・5H 2 O 0.8 〃 ZnSO 4・7H 2 O 4.0 〃 MnCl 2・4H 2 O 4.0 〃 *Culture was started at an initial concentration of 150 g/, and 100 g/ was added after 45 hours. After the culture was completed, the cells were treated in the same manner as in Example 1 to obtain 58.01 dry cells. This bacterial cell was extracted in the same manner as in Example 1 to obtain γ-linolenic acid.
14.7 g of lipid containing 34.4% was obtained. The fatty acid composition of the obtained lipids is shown in Table 4. Table 4 Fatty acid content (%) 16:0 10.6 18:0 2.3 18:1 27.4 18:2 18.1 18:3 34.4 Example 3 In Example 2, instead of Mucor circinelloides HUT 1121 (FERM P-9359) Fifth
The same procedure as in Example 2 was carried out except that the microorganisms shown in the table were used. Table 5 shows the bacterial cell yield, γ-linolenic acid content, and lipid yield.
【表】
比較例 １
グルコース200g／、硫酸アンモニウム
13.2g／、リン酸１カリウム9g／、MgSO4・
7H2O 1g／、酵母エキス0.6g／、FeSO4・
7H2O 30mg／、CaCl2・2H2O 3.6mg／、
CuSO4・5H2O 0.6mg／、ZnSO4・7H2O 3.0
mg／、MnCl2・4H2O 3.0mg／を含む倍地
（PH4.0）６を用い、この倍地にモルテイエレ
ラ・ラマニアナ・バル・アングリスポラ
（Mortiere−lla ramanniana var.angulispora）
（IFO 8187）を接種し、以下実施例１と同様に行
なつた。その結果、乾燥菌体として91.5g／の
菌体を得た。この菌体から抽出された脂質は
26.04g／であつたが、γ−リノレン酸含量は
8.19％であつた。
比較例 ２
比較例１と同じ組成の倍地に比較例１と同じ微
生物を接種し、同じ条件で培養を行ない、45時間
経過後にグルコース60g／を追加したこと以外
は比較例１と同様に行なつた。その結果、乾燥菌
体として96.7g／の菌体を得た。この菌体から
抽出された脂質は43.1g／であつたが、γ−リ
ノレン酸含量は8.4％であつた。
［発明の効果］
本発明によれば、攘酵法によつてγ−リノレン
酸を高率で含有する脂質を効率よく得ることがで
きる。γ−リノレン酸を含む脂質は食品、医薬品
等の分野において広範な利用が期待される。[Table] Comparative example 1 Glucose 200g/, ammonium sulfate
13.2g/, monopotassium phosphate 9g/, MgSO 4 .
7H 2 O 1g/, yeast extract 0.6g/, FeSO 4 .
7H 2 O 30mg/, CaCl 2・2H 2 O 3.6mg/,
CuSO 4・5H 2 O 0.6mg/, ZnSO 4・7H 2 O 3.0
Using a medium (PH4.0) 6 containing MnCl 2 4H 2 O 3.0 mg/, Mortiere-lla ramanniana var.angulispora (Mortiere-lla ramanniana var.angulispora)
(IFO 8187) and the same procedure as in Example 1 was carried out. As a result, 91.5 g of dried bacterial cells were obtained. The lipids extracted from this bacterial body are
26.04g/, but the γ-linolenic acid content was
It was 8.19%. Comparative Example 2 The same microorganisms as in Comparative Example 1 were inoculated into a medium with the same composition as in Comparative Example 1, cultured under the same conditions, and 60 g/g of glucose was added after 45 hours. Summer. As a result, 96.7 g of dried bacterial cells were obtained. The amount of lipid extracted from this bacterial cell was 43.1 g/g, but the γ-linolenic acid content was 8.4%. [Effects of the Invention] According to the present invention, a lipid containing a high percentage of γ-linolenic acid can be efficiently obtained by a fermentation method. Lipids containing γ-linolenic acid are expected to be widely used in fields such as food and medicine.
Claims (1)
circinelloides）またはムコール・ジヤバニクス
（Mucor javanicus）を炭素源として糖類または
酢酸ソーダを含む培地に培養し、培養物からγ−
リノレン酸を30％以上含有する脂質を採取するこ
とを特徴とするγ−リノレン酸含有脂質の製造
法。 ２ 炭素源がグルコースである特許請求の範囲第
１項記載の方法。 ３ ムコール・シルシネロイデス（Mucor
circinelloides）またはムコール・ジヤバニクス
（Mucor javanicus）を炭素源として糖類または
酢酸ソーダを含む培地に培養し、培養中に炭素源
を追加して培養を行い、培養物からγ−リノレン
酸を30％以上含有する脂質を採取することを特徴
とするγ−リノレン酸含有脂質の製造法。 ４ 炭素源がグルコースである特許請求の範囲第
３項記載の方法。 ５ 炭素源の初発濃度が50〜250ｇ／である特
許請求の範囲第３項記載の方法。[Claims] 1. Mucor circinelloides (Mucor
circinelloides or Mucor javanicus was cultured in a medium containing sugar or sodium acetate as a carbon source, and γ-
A method for producing a γ-linolenic acid-containing lipid, which comprises collecting a lipid containing 30% or more of linolenic acid. 2. The method according to claim 1, wherein the carbon source is glucose. 3 Mucor circinelloides (Mucor
circinelloides) or Mucor javanicus in a medium containing sugar or sodium acetate as a carbon source, and the carbon source is added during the culture to produce a culture containing 30% or more of γ-linolenic acid. A method for producing a γ-linolenic acid-containing lipid, the method comprising collecting a lipid containing γ-linolenic acid. 4. The method according to claim 3, wherein the carbon source is glucose. 5. The method according to claim 3, wherein the initial concentration of the carbon source is 50 to 250 g/.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62118881A JPS63283589A (en) | 1987-05-18 | 1987-05-18 | Production of gamma-linolenic acid-containing lipid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62118881A JPS63283589A (en) | 1987-05-18 | 1987-05-18 | Production of gamma-linolenic acid-containing lipid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63283589A JPS63283589A (en) | 1988-11-21 |
| JPH058677B2 true JPH058677B2 (en) | 1993-02-02 |
Family
ID=14747442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62118881A Granted JPS63283589A (en) | 1987-05-18 | 1987-05-18 | Production of gamma-linolenic acid-containing lipid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63283589A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63263088A (en) * | 1987-04-20 | 1988-10-31 | Lion Corp | Gamma-linolenic acid-containing intracellular lipid |
-
1987
- 1987-05-18 JP JP62118881A patent/JPS63283589A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63263088A (en) * | 1987-04-20 | 1988-10-31 | Lion Corp | Gamma-linolenic acid-containing intracellular lipid |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63283589A (en) | 1988-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shinmen et al. | Production of arachidonic acid by Mortierella fungi: selection of a potent producer and optimization of culture conditions for large-scale production | |
| AU2009213103B2 (en) | Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors | |
| JP6169150B2 (en) | Process for producing highly unsaturated fatty acids using novel strain preservation technology | |
| AU2008338017B2 (en) | Method for the cultivation of microorganisms of the order thraustochytriales | |
| JP3995290B2 (en) | Method for producing omega-9 polyunsaturated fatty acid and lipid containing the same | |
| JP4088097B2 (en) | Method for producing highly unsaturated fatty acid-containing lipid | |
| EP0159137A2 (en) | Microbial desaturase enzyme inhibitors and their use in a method of producing lipids | |
| JPH058677B2 (en) | ||
| Blakley | Citrate formation in Mycobacterium phlei | |
| JPH0378106B2 (en) | ||
| CN117604046A (en) | Single-cell grease production process | |
| KR910000453B1 (en) | Novel microorganism streptomyces sp kccb2 | |
| JP2008307063A (en) | PROCESS FOR PRODUCING ARACHIDONIC ACID-CONTAINING LIPID AND DIHOMO-gamma-LINOLENIC ACID CONTAINING LIPID | |
| Ziniades | The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum | |
| JPS6251110B2 (en) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| EXPY | Cancellation because of completion of term | ||
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20080202 Year of fee payment: 15 |