JPH0580044A - Blood separation agent and blood separation tube - Google Patents

Abstract

PURPOSE:To obtain a blood separation agent excellent in practice by using a separation layer forming material, a lipophilic laminar inorganic based com pound and a viscosity applying agent. CONSTITUTION:A separation layer forming material herein used has a specific weight in an intermediate range between a serum component and a blood cell component and a high polymer oil-like matter with the viscosity at 25 deg.C in a range of 200-600,000 CPS is suitable, for example, silicone and chlorinated polybutene. A lipophilic laminar inorganic based compound brings low and medium molecular organic compound between layers to expand easily and with high thixotropy, it is added to the separation forming material at a rate of 0.1-5 pts.wt. per 100 pts.wt. thereof. A viscosity applying agent such as rosin or dammar is blended into a high polymer oil-like matter to improve adhesiveness and wettability with an object to be applied. The agent is added to the separation forming material at the ratio of 0.1-60 pts.wt. per 100 pts.wt. thereof. In the preferable physical property of compositions as mentioned above composing a blood separation material, specific gravity at 25 deg.C is intermediate between the serum and blood cell, namely, 1.035-1.06 and the viscosity is appropriately in a range of 200,000-2,000,000 CPS.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a blood separating agent.
More specifically, when separating the serum part and blood clot part in blood, or the plasma part and blood cell part by their specific gravity differences, a partition wall is formed between both components by giving an intermediate specific gravity of both components. The present invention relates to a blood separating agent that can be used for the purpose of facilitating the separation operation of these both components, and a blood separating tube containing the same in a bottomed tube.

[0002]

2. Description of the Related Art Conventionally, as a separating agent used for separating blood, a resin such as silicone oil, chlorinated polybutene, polyisobutene, an acrylic polymer or an α-olefin / maleic acid diester polymer is used as a main component. In some of these, inorganic fine particles such as silica and clay or an organic gelling agent were added for adjusting specific gravity and viscosity. these,
The resin as the main component is basically hydrophobic, and the inorganic fine particles give a thickening effect and a thixotropy imparting property, and the organic gelling agent thickens without imparting the thixotropy property. It gives an effect.

However, the resins as the main components are liquid, and if the separation tube housed in the bottomed tube is stored horizontally, the separating agent will flow out over time, which is not practical. In order to compensate for such drawbacks, a thixotropic agent such as finely pulverized mica and colloidal silica, an organic gelling agent and the like are added. However, in a system to which a thixotropy-imparting agent is added, it has thixotropy at room temperature and can suppress the flow at the time of storage, but at high temperature the thixotropy is reduced and the viscosity is also low, so that separation The agent will flow. Further, the system to which the organic gelling agent is added has a drawback that the flow is suppressed at room temperature and high temperature, but the viscosity as time passes and the performance as a separating agent is deteriorated.

[0004]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a blood separating agent which is free from these drawbacks and which is excellent in practical use, and a blood separating tube which is obtained by accommodating the blood separating agent in a bottomed tube. .. As a result of intensive studies to achieve such an object, the present inventors prevent the flow regardless of temperature by coexisting a lipophilic layered inorganic compound and a tackifier in a separation layer forming material. The present invention has been discovered and the present invention has been achieved.

[0005]

That is, the present invention is a blood separating agent containing a separating layer forming material, a lipophilic layered inorganic compound and a tackifier, and further, the blood separating agent is housed in a bottomed tube. It is a blood separation tube made of.

The present invention will be described in detail below. As the separation layer molding material used for the blood separating agent of the present invention, as long as its specific gravity is in the intermediate region between the serum component and the blood cell component, a high-viscosity polymer oil from various low-viscosity organic solvents or plasticizers Even things can be used. Practically, a polymer oil having a viscosity in the range of 200 to 600,000 cps at a temperature of 25 ° C. is preferable from the viewpoint of providing stable and appropriate fluidity and gelation property. Examples of such polymer oils include silicone, chlorinated polybutene, chlorinated polybutadiene, poly (meth) acrylic acid ester, polyisobutene, α-olefin or a copolymer of styrene and maleic acid diester. ..

The lipophilic layered inorganic compound used in the blood separating agent of the present invention means a metal ion existing between clay minerals such as natural and synthetic mica, mica, bentonite and smectite, and lipophilic oil such as alkyl ammonium salt. It is an onium salt having a group. These lipophilic layered inorganic compounds take in low- and medium-molecular-weight organic compounds between layers and easily swell. This makes high thixotropy
You can have sex. Furthermore, due to temperature changes,
The oil absorbency changes, and it is possible to reduce the change in thixotropy due to temperature change. These lipophilic layered inorganic compounds must be blended in consideration of the viscosity and specific gravity of the separating agent, but normally it is desirable to add 0.1 to 5 parts by weight to 100 parts by weight of the separating layer forming material. .. If the amount of the lipophilic layered inorganic compound added is too small, sufficient thixotropy cannot be imparted, which causes flow. On the other hand, if the amount added is too large, the viscosity and specific gravity increase, which is not suitable for practical use.

The tackifier used in the blood separating agent of the present invention is generally thermoplastic, is solid or semi-solid at room temperature, has a softening point of about 5 to 150 ° C., and has a molecular weight of about 200.
It is about 1500. By blending these tackifiers in a polymer oil, they act viscoelastically and interfacially, and dramatically improve the adhesiveness and wettability with the adherend.
These tackifiers are largely classified into natural resin tackifiers and synthetic resin tackifiers. The tackifier is a compounding agent that is added to the polymer to impart tackiness, and has a low glass transition point and a low softening point,
Many give liquidity.

Examples of natural resin tackifiers include rosin, dammar, polymerized rosin, partially hydrogenated rosin, glycerin ester rosin, glycerin ester rosin partially hydrogenated product, glycerin ester rosin complete hydrogenated product, polymerized glycerine ester rosin, Pentaerythritol ester rosin, partially hydrogenated pentaerythritol ester rosin, polymerized pentaerythritol ester rosin, α-pinene, β
-Polyene polymers such as pinene polymers and dipentene polymers, hydrogenated products of polyterpene resins, and terpene phenols. Synthetic resin-based tackifiers include olefin and diolefin polymers, cyclopentadiene resins, aromatic petroleum resins, hydrogenated aromatic resins, phenol resins, alkylphenol-acetylene resins, styrene resins. , Xylene-based resin, coumarone-indene resin, vinyltoluene-α-methylstyrene copolymer resin and the like. These tackifiers must be blended in consideration of compatibility with the separation layer forming material, viscosity, etc., but usually 0.1 to 60 parts by weight, preferably 100 parts by weight of the separating layer forming material, preferably It is desirable to add 1 to 30 parts by weight. If the addition amount of the tackifier is too small, the amount of oil absorption to the lipophilic layered inorganic compound is small and sufficient thixotropy cannot be obtained. Further, if the addition amount is too large, the compatibility becomes poor, leading to separation,
Viscosity increases, which adversely affects centrifugation.

The preferred physical properties of the composition constituting the blood separating agent of the present invention are that the specific gravity at a temperature of 25 ° C. is between serum and blood cells, ie, 1.035 to 1.060, and the viscosity is 20.
The range of 10,000 to 2,000,000 cps is suitable. The blood separating agent according to the present invention may optionally contain inorganic powders such as silica, talc and alumina, organic additives such as wax, plasticizer and gelling agent, blood coagulant and the like. As the method for producing the blood separating agent of the present invention, the separating layer molding material is heated to a temperature of 80 to 200 ° C., a predetermined amount of the lipophilic layered inorganic compound and the tackifier are added thereto, and the mixture is heated and stirred for several hours. Alternatively, there is a method of dispersing with a roll.

Next, the present invention will be described more specifically by way of examples, but the present invention is not limited to the following examples unless it exceeds the gist. In the examples,
Unless otherwise specified, "part" represents part by weight.

Example 1 A mixed α-olefin having 12 and 14 carbon atoms and a maleic acid dimethyl ester copolymer 100 were placed in a four-necked flask.
Part, 5 parts of pentaline H (pentaerythritol ester of partially hydrogenated rosin: manufactured by Rika Hercules),
The mixture was stirred and mixed at 100 ° C. for 1 hour. This is Benton 3
8 (Alkyl ammonium modified natural smectite: NL
2 parts of Industrie Co., Ltd. and three parts were kneaded together to obtain a blood separating agent having a viscosity of 400,000 cps and a specific gravity of 1.040. The obtained separating agent was put in the bottom of a 15 cc test tube in an amount of 1.5 g, and was placed sideways in a dryer at 60 ° C. for 4 days. As a result, the flow was 2 mm or less.

Example 2 Polyisobutene 1 having a molecular weight of about 1000 was placed in a four-necked flask.
Charge 100 parts of steberite 10 (5 parts of glycerin ester of partially hydrogenated rosin: manufactured by Rika Hercules) and
The mixture was stirred and mixed at 00 ° C. for 1 hour. This is Benton 38
(Alkyl ammonium modified natural smectite: manufactured by NL Industry Co., Ltd.) and 3 parts were kneaded together to obtain a blood separating agent having a viscosity of 400,000 cps and a specific gravity of 1.048. The obtained separating agent was put in the bottom of a 15 cc test tube in an amount of 1.5 g, and was placed sideways in a dryer at 60 ° C. for 4 days. As a result, the flow was 2 mm or less.

Example 3 Mixed α-olefin having 12 and 14 carbon atoms, 100 parts of maleic acid dimethyl ester copolymer and YS resin Px
# 1000 (Terpine resin: Yasuhara Yushi Kogyo Co., Ltd.) 20
And 3 parts of CR-TS (alkylammonium-modified mica: manufactured by Topy Industries, Ltd.) were kneaded with 3 rolls to give a viscosity of 50.
A blood separating agent with 10,000 cps and a specific gravity of 1.042 was obtained. The obtained separating agent was placed in a 15 cc test tube in an amount of 1.5 g, and was placed sideways in a dryer at 60 ° C. for 4 days. As a result, the flow was 1 mm or less.

Comparative Example 1 100 parts of a mixed α-olefin having 12 and 14 carbon atoms, a dimethyl ester of maleic acid copolymer and 38 of benton.
(Alkyl ammonium modified natural smectite: manufactured by NL Industry Co., Ltd.) 2.2 parts, colloidal silica 0.5
The parts were kneaded with three rolls to obtain a blood separating agent having a viscosity of 600,000 cps and a specific gravity of 1.045. The separating agent obtained is 1
When 1.5 g was put in a 5 cc test tube and laid down and left in a dryer at 60 ° C. for 1 day, the flow was 8 mm.

Comparative Example 2 100 parts of a mixed α-olefin having 12 and 14 carbon atoms, a dimethyl ester of maleic acid copolymer and pentaline H
(Partially hydrogenated rosin pentaerythritol ester: manufactured by Rika Hercules Co., Ltd.) 40 parts was placed in a four-necked flask and mixed with stirring at 100 ° C. for 2 hours to give a viscosity of 2,000,000 cps.
A blood separating agent having a specific gravity of 1.035 was obtained. The obtained separating agent was placed in a 15 cc test tube in an amount of 1.5 g, and when it was laid down and left standing at room temperature for 1 day, the flow was 60 mm.

Comparative Example 3 100 parts of a mixed α-olefin having 12 and 14 carbon atoms, a dimethyl ester of maleic acid copolymer and Gelol D
0.5 parts (condensate of sorbitol and benzaldehyde, organic gelling agent: manufactured by Shin Nippon Rika Co., Ltd.) were kneaded with 3 rolls, and blood was separated with a specific gravity of 1.050 and a viscosity of 500,000 cps. I got an agent. The obtained separating agent is 1.5 in a 15 cc test tube.
It was placed in a dryer at 60 ° C. for 4 days, and the flow was less than 1 mm.
The viscosity increased with time, and after one year, it completely stopped flowing even after centrifugation.

[0018]

EFFECTS OF THE INVENTION The blood separating agent according to the present invention has a room temperature, compared with a conventional inorganic powder which does not use a lipophilic layered inorganic compound and a tackifier together, or a blood separating agent containing an organic gelling agent. Also, there is little flow over time during heating, and there is little change in viscosity over time. It has a thixotropic property that is not affected by temperature, which is not present in conventional blood separating agents, and therefore, even if the viscosity is not high, it does not change the shape of the separating agent with time, nor does it change with time. In addition, a blood collection tube having excellent physical properties and stability can be provided.

Claims (3)

[Claims] 1. A blood separating agent comprising a separating layer forming material, a lipophilic layered inorganic compound and a tackifier. 2. The blood separating agent according to claim 1, which has a specific gravity at 25 ° C. of 1.030 to 1.060. 3. A blood separation tube containing the blood separation agent according to claim 1 in a bottomed tube.