JPH0576341A - Cell transfer apparatus - Google Patents

Abstract

PURPOSE:To perform an operation such as selection and collection of necessary organism cell and the removal of dust, etc., by attaching a sucking and discharging pump to a cube having small holes on the side face, rotating the cube to suck a cell from a liquid phase and releasing the cell at the end of rotation. CONSTITUTION:A collection drum 6 is rotated in a Petri dish 3 holding a solution 4 containing organism cells. Sucking and discharging pumps 14a... are connected to connection holes 12a... connected to small holes on the side faces of the drum. When a small hole is immersed in the liquid phase, a negative pressure is applied to the small hole to suck a cell, which is released by applying a positive pressure to the small hole at the transfer end of rotation.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cell transporting device for individually collecting, sorting, transporting, etc. cells individually isolated and dispersed in a liquid phase.

[0002]

2. Description of the Related Art In the field of so-called biotechnology, techniques for growing new varieties of plants by cell fusion have been developed. Conventionally, as such a cell manipulation means, one disclosed in the Journal of Precision Engineering (56/1/1990) is known. According to this conventional technique, as shown in FIG. 8, a structure called a micro-chamber plate is formed by forming a large number of fine holes 1 on a single crystal silicon substrate by utilizing an etching technique in a semiconductor process. use.

Each hole 1 has a quadrangular pyramid taper shape on the upper side and a slit on the lower side, and a pair of electrodes (not shown) are provided on both sides of each slit. Then, two different types of cells having a diameter of about 20 to 100 μm are supplied to the microchamber plate 2 from the upper side by the flow of an isotonic solution, and the negative voltage applied to the above electrodes sucks and charges the slits of the holes 1. To wear. Next, while a pair of cells to be fused are in contact with each other and fixed on the slit, by applying a pulse to the electrode,
It breaks the membrane at the contact area of a pair of cells and initiates cell fusion.

[0004]

However, in such a conventional cell manipulating means, as shown in FIG. 8, the microchamber plate has a large number of fine holes suitable for the size of the cells. The semiconductor process technology is applied to perform this fine processing.
This microchamber plate itself does not have a selection function of selecting a pair of cells to be fused and fixing them in their respective pores, but simply as a result of coincidence when they are supplied together with the liquid phase. It just determines the combination of cells.

Therefore, it goes without saying that the cells cannot be individually discriminated and judged, and in the case of plant cells, dust such as leaf vein pieces cannot be separated from the required cells for use. Unable to exclude unnecessary things such as. Furthermore, if you try to select cells continuously,
There is a problem that it is necessary to facilitate a large number of microchamber plates and operate each microchamber plate by some kind of transfer means.

The present invention has been made in view of such conventional problems, and it is possible to select and select necessary cells by identifying individual cells and individually collecting and transporting them.
It is an object of the present invention to provide a cell transfer device capable of performing fine processing such as removing dust.

[0007]

In order to achieve such an object, the present invention provides a collecting drum such as a cylinder or a polygonal column having micropores having a size of about the diameter of cells on the side surface of the collecting drum. A means for alternately applying negative pressure and positive pressure air from the inside to the outside of the micropores is provided, and the sampling drum is continuously rotated in a state in which the side end of the sampling drum is immersed in a liquid phase containing cells, At the timing when the micropores are immersed in the liquid phase, a negative pressure is applied to the micropores by the above-mentioned means to suck the cells into the micropores, and at the timing when the micropores rise from the liquid phase, the aspirated cells are subjected to image discrimination processing. If it is determined that the micropores are not suitable by this selection, the micropores are removed from the micropores by positive pressure by the above means, and if it is determined that they are suitable, the micropores are positive pressure by the above means. By reason And to convey a predetermined receiving unit.

[0008]

With this configuration, even when processing a large number of types of cells, it is possible to continuously select and select predetermined cells, so that it is possible to collect and sort with extremely high accuracy. , The process of transportation can be performed automatically.

[0009]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. First, the configuration of the apparatus will be described with reference to FIGS.
In FIG. 1, reference numeral 3 denotes a chassis in which a solution 4 containing cells is placed, and the chassis 3 is mounted on a movable table 5. Reference numeral 6 is a cylindrical sampling drum, and four sampling pieces 7a ...
7d are fixed at regular intervals along the circumferential direction. As shown in FIG. 3, each of the sampling pieces 7a to 7d has a structure in which a microscopic hole 9 is formed by subjecting a single crystal silicon substrate 8 to an etching treatment in a semiconductor process technology, and the microscopic hole 9 has a microscopic hole 9. It is fixed so as to face the outer side in the radial direction of 6.

As shown in the longitudinal sectional view of FIG. 2, the sampling drum 6 is fixed to a drive shaft 11 of a rotation drive motor 10 and is rotationally driven in the circumferential direction about the central axis of the rotary drive motor 10. One end on the side is driven to rotate so as to be immersed in the solution in the Petri dish 3. Furthermore, inside the sampling drum 6,
As shown in FIG. 2, the micropores 9 in each of the collected pieces 7a to 7d.
Communication holes 12a to 2d (in FIG. 2, 12a and 2d) communicating with
(indicated by c) are formed independently of each other, and each of the communication holes 12a is formed.
The pressure sensor 13a is provided at one end of the two forks 12a to 12d.
13d is fixed, and the pressurizing / depressurizing pump 14a is attached to the other end.
14d is provided. That is, each of the collected pieces 7a to 7
4 individual communication holes 12a to 12 for d respectively
d, pressure sensors 13a to 13d and pressurizing / depressurizing pump 14a.
.About.14d are provided.

Reference numeral 15 denotes an electric brush for electrically connecting the pressure sensors 13a to 13d and the pressurizing / depressurizing pumps 14a to 14d to a control unit 19 which will be described later.
Even if 3a to 13d and the pressurizing / depressurizing pumps 14a to 14d rotate together with the sampling drum 6, signals can be exchanged by always connecting to the control unit 19.

Referring again to FIG. 1, reference numerals 15 and 16 denote chambers, which are provided so as to oppose and do not come in contact with the traces of the moving shafts of the collecting pieces 7a to 7d of the collecting drum 6, and further to collect the respective collecting pieces 7a to 7d. It is provided at an angle of 90 ° according to the installation phase of 7d. Reference numeral 17 is a video camera, and as shown in FIG. 1, when two pieces are opposed to the chambers 15 and 16 and the other piece is immersed in the solution 4 in the dish 3, It is installed in such a positional relationship that the image of the collected piece fits within the angle of view.

Reference numeral 18 is a photographing lamp for setting the optimum exposure of the video camera 17. Reference numeral 19 denotes a control unit, which reproduces an image on the CRT display 20 based on the image signal from the video camera 17, performs image signal processing described later based on the image signal, and further controls the drive of the drive motor 10. The signal M is output and each pressurizing / depressurizing pump 1
4a to 14d operation timing and each pressure sensor 13
Based on the analysis result of the detection signal from, the control such as outputting the control signals S1 to S4 for performing the optimum control is performed.

Next, the operation of the embodiment having such a configuration will be described. The control unit 19 provides the drive motor 10 with the control signal M to continuously rotate the sampling drum 6 in steps of 90 °, and the collection pieces 7a to 7d of the petri dish 3 are sequentially rotated by 90 °. Immerse in solution 4.
The processing operation by the collection piece 7a will be described as a representative. When the collection piece 7a or the solution 4 in the dish 3 is immersed, the control unit 19
The control signal Sa from the pressurizing / depressurizing pump 14a starts the depressurizing operation, and the inside of the micropores of the sampling piece 7a becomes negative pressure. As a result, the cells contained in the solution 4 are suctioned to the tip of the collection piece 7a. Here, the inside diameter of the micropore 9 of the sampling piece 7a is 1
Since the diameter is smaller than the diameter of one cell, this suction operation causes only one cell to be adsorbed to the tip of the collection piece 7a.

Next, when the sampling drum 6 is rotated by 90 °, the video camera 17 photographs the cells in the state of being sucked at the tip of the sampling piece 7a, and the video signal is transmitted to the control unit 19.
The control unit 19 compares the image data of a predetermined type of cell set inside beforehand with the transmitted image signal by image processing such as a pattern matching method, and determines that the predetermined criteria are satisfied. In this case, it is determined that these cells should be collected, and if they have a shape that does not satisfy the standard such as dust or abnormal cells, they should be discarded. When the sampling drum 6 further rotates 90 °, the sampling piece 7a comes to face the chamber 15 and when it is determined that the sampling should be performed, the control unit 19 causes the pressurizing / depressurizing pump 14a to perform a pressurizing operation to perform sampling. The cells adsorbed on the piece 7a are ejected to the collection chamber 15 side. On the other hand, when it is determined that the collection chamber 1 is to be discarded, the pressurization / depressurization pump 14a continues the depressurization operation at this point to collect dust and unnecessary cells.
Do not enter 5.

When the sampling drum 6 further rotates 90 °, the sampling piece 7a faces the chamber 16, and the control unit 19 causes the pressurizing / depressurizing pump 14a to perform the pressurizing operation, so that the dust adsorbed on the sampling piece 7a. Then, unnecessary cells are discharged to the discarding chamber 16 side. The pressure sensor 13a constantly detects the pressure in the communication hole 12a by the pressurizing / depressurizing pump 14a, and transmits the detection signal Pa to the control unit 19 so that the control unit 1
9 is a pressurizing / depressurizing pump 14 so as not to destroy the collected cells.
a is automatically controlled.

The pressurizing / depressurizing pumps 14b to 14d associated with the other sampling pieces 7b to 7d also operate to pressurize and depressurize in accordance with the rotation position of the sampling drum 6, and the collected cells are imaged by the video camera 17, and their images are taken. The quality of the cells, dust, etc. are judged from the signal, and only cells satisfying a predetermined standard are conveyed to the chamber 15, and unnecessary substances are discarded to the chamber 16.

As described above, according to this embodiment, cells are collected and sorted by adhering cells one by one to the sample piece by the suction force of the pressurizing / depressurizing pump, and only suitable cells are transferred to a predetermined chamber. Since the continuous treatment of cells is automated, highly precise cell treatment can be realized. Next, another embodiment will be described with reference to FIGS.

In FIG. 4, 21 is a solution 2 containing cells.
It is a petri dish that holds 2 and is placed on the movable table 23. Reference numeral 24 denotes a hollow cylindrical sampling drum, and four sampling pieces 25a to 25d are fixed to its side end surface at equal intervals along the same circumferential direction. Collected pieces 25a to 25d
3 has a structure in which micropores having an inner diameter smaller than the diameter of cells are formed by subjecting a single crystal silicon substrate to etching treatment of a semiconductor process technology, as shown in FIG. They are fixed so as to face outward in the radial direction of 24. Further, each of the collected pieces 25a-25
The minute hole of d and the hollow portion of the sampling drum 24 communicate with each other through minute through holes 24a to 24d.

The sampling drum 24, as shown in FIG.
It is rotatably supported by a piping mechanism 26 inserted from the back side along the central axis. That is, the piping mechanism 26
It has a portion to be inserted into the hollow portion of the sampling drum 24 and a support portion that rotatably supports the sampling drum 24 via a bearing 27, and is integrally fixed to a body 28 of the apparatus.
Then, the gear mechanism 2 provided at one end of the sampling drum 24
By connecting to the drive shaft of the rotary drive motor 30 via 9, the sampling drum 24 is driven by the drive force of the drive motor 30.
It is rotationally driven so that one end on the circumferential side is immersed in the solution 22 in the petri dish 21.

In FIG. 4, reference numerals 31 and 32 denote chambers, and the sampling drum 24 is arranged at an angle of 90 ° so as to face each of two adjacent sampling pieces each time the sampling drum 24 rotates by 90 °. Is provided so as to face the side edge of the. Reference numeral 33 denotes a video camera, which is arranged at a position opposite to the chamber 32. Therefore, when the two collection pieces face the chambers 31 and 32 and one collection piece is immersed in the liquid 22 of the petri dish 21, the remaining collection pieces are set within the angle of view of the video camera 33. It is configured.

Reference numeral 34 is a photographing lamp for setting the optimum exposure of the video camera 33. Further, to explain the structure of the piping mechanism 26, the portion inserted into the hollow of the sampling drum 24 has a smaller diameter than the inner diameter of the hollow, and the gap formed as a result is the chamber 35. A communication hole 37 that communicates the pressure reducing motor 36 and the chamber 35 provided outside is formed. Furthermore, the sampling drum 24
Is rotated in the circumferential direction, the four through holes 24a ...
Two projections 26a, 26b are formed so as to slide on the inner wall of the sampling drum 24 so as to trace the movement track of 24d, and these projections 26a, 26b are two as shown in FIG. When the collection piece and the chambers 31 and 32 face each other, they are arranged at an angle of 90 ° so as to face them from the inside of the collection drum 24. Then, the protrusion 26
A second communication hole 38a is formed which communicates with the external pressurization / decompression pump 39 from the tip opening of a, and communicates with another external compression / decompression pump (not shown) from the tip opening of the protrusion 26b. A third communication hole 38b is formed. Therefore, as shown in FIG. 4, when the chambers 31 and 32 and the two collection pieces (for example, 25c and 25d face each other, the chamber 31 and the second communication hole 38a communicate with each other, the chamber 32
And the third communication hole 38b communicate with each other.

Reference numeral 40 is a pressure sensor provided at one end of the first communication hole 37 for measuring the internal pressure, and 41 is a pressure sensor provided at one end of the second communication hole 38 for measuring the internal pressure. , The control signals of the respective measurement signals P1 and P2
2 to transmit. Although not shown, the third communication hole 38b
Is also provided with a pressure sensor and transmits the measurement signal P3 to the control unit 42.

The control section 42 is provided with a calculating means such as a microprocessor, receives the measurement signals P1, P2 and P3, and determines the first, second and third communication holes 37, which are predetermined.
Control signals S1 for setting the internal pressures of 38a and 38b
S2 and S3 are transmitted to the respective pumps 36 and 39 and a pump (not shown). Further, the control signals S2 and S3 are used to instruct the pressurizing / depressurizing pump 39 and a pressurizing / depressurizing pump (not shown) for pressurizing operation and depressurizing operation. The control unit 42 also has a function of receiving a video signal from the video camera 33, performing image processing described later, and distinguishing dust or abnormal cells from normal cells.

Next, the operation of another embodiment having such a configuration will be described. First, the decompression pump 36 is set to always perform decompression operation at a constant pressure. Further, by supplying the control signal M at a predetermined timing from the control unit 42 to the rotary drive motor 30, the sampling drum 24 is continuously stepwise rotated by 90 °, and the sampling pieces 24a to 24d are sequentially arranged in the petri dish 2.
Immerse in the solution 22 in 1.

The processing operation by the collection piece 24a will be described as a representative example. When the collection piece 24a is immersed in the solution 22 in the dish 21, one cell is sucked into the micropores of the collection piece 24a by the negative pressure in the chamber 35. It Next, there are 9 sampling drums
When rotated by 0 °, the video camera 33 images the cells still adsorbed on the collection piece 24a, and transmits the video signal to the control unit 42. The control unit 42 compares the image data of a cell of a predetermined type set inside beforehand with the transmitted image signal by image processing, determines that the cell is a normal cell if it meets a predetermined criterion, and collects it. Judge that it should. On the other hand, those that do not meet the criteria are judged to be abnormal cells or dust and are judged to be discarded.

Next, when the sampling drum 24 is rotated by 90 °, the sampling piece 7a faces the chamber 31.
At the time of this switching, the pressurizing / depressurizing pump 32 and the pressurizing / depressurizing pump (not shown) are depressurized, and when the control unit 42 determines that the cells are normal cells, the depressurizing operation is continued. Therefore, the normal cells remain adsorbed on the collected piece 24a. On the other hand, when it is determined that the cells are abnormal cells or dust, the pressurizing / depressurizing pump 39 is switched to the pressurizing operation. Therefore, the second
As the pressure inside the communication hole 38a increases, abnormal cells and the like are discharged into the discarding chamber 31 and are discarded.

Next, when the sampling drum 24 is further rotated 90 °, the sampling piece 25a faces the sampling chamber 32,
In the case of normal cells, the pressurizing / depressurizing pump (not shown) is switched to the pressurizing operation for the first time at this opposing time point. As a result,
Normal cells are discharged into the chamber 32 and collected. Although the sampling operation for the sampling piece 25a has been described above, the pressurization / depressurization pump 39 and the pressurization / decompression pump (not shown) for the other sampling pieces 25b to 25d also perform the pressurization / decompression operation according to the rotation position of the sampling drum 25, and the sampling operation is performed. The imaged cells are imaged by the video camera 33, the quality of the cells, the dust, etc. are judged from the video signal, and only the cells satisfying the predetermined standard are transported to the collection chamber 32, and unnecessary materials are discarded. Discard into the chamber 31 for use.

Next, still another embodiment will be described with reference to FIGS. This embodiment corresponds to a modification of the embodiment shown in FIGS. 4 and 5. That is, in the second embodiment, the sampling drum is rotated in a direction perpendicular to the petri dish, whereas in this embodiment, the collection drum is horizontally rotated in the casing of the apparatus. In FIG. 6, 42 is a space corresponding to a petri dish formed in the casing. Reference numeral 43 denotes a hollow cylindrical sampling drum, and four sampling pieces 44a to 44d are fixed to the side end surface of the sampling drum at equal intervals along the same circumferential direction. Each of the collected pieces 44a to 44d has a structure in which micropores having an inner diameter smaller than the diameter of the cell are formed by subjecting the single crystal silicon substrate to etching treatment of semiconductor process technology, as in the case shown in FIG. The minute holes are fixed so as to face the outside of the sampling drum 43 in the radial direction. Further, the minute holes of the respective collecting pieces 44a to 44d and the hollow portion of the collecting drum 43 are minute through holes 45a.
~ 45d communicates.

Further, the sampling drum 43, as shown in FIG.
It is horizontally rotatably supported by a piping mechanism 46 inserted from the back side along the central axis. That is, the piping mechanism 46
Has a portion to be inserted into the hollow portion of the sampling drum 43 and a support portion that rotatably supports the sampling drum 43 via a bearing 47, and is integrally fixed to a body 48 of the apparatus. Then, by connecting to the drive shaft of the rotary drive motor 50 via a gear mechanism 49 provided at one end of the sampling drum 46, the sampling drum 43 is driven by the driving force of the drive motor 50.
Is rotationally driven so that one end on the circumferential side is immersed in the solution in the Petri dish portion 42.

In FIG. 7, 51 and 52 are both chambers, and the sampling drum 43 is arranged at an angle of 90 ° so as to face each of two adjacent sampling pieces each time the sampling drum 43 rotates by 90 °. Is provided so as to face the side edge of the. Reference numeral 53 denotes a video camera, which is arranged at a position opposite to the chamber 52 through a transparent window 54. Therefore, the two collection pieces face the chambers 51 and 53, and
When the individual sample pieces are immersed in the petri dish part 42, the remaining sample pieces are configured to fit within the angle of view of the video camera 53.

Reference numeral 55 is a photographing lamp for setting the optimum exposure of the video camera 53. Further, to explain the structure of the piping mechanism 46, the portion inserted into the hollow of the sampling drum 43 has a smaller diameter than the inner diameter of the hollow, and the resulting gap forms the chamber 56. Then, a first communication hole 58 that communicates the pressure reducing motor 57 and the chamber 56 provided outside is formed. Furthermore, when the sampling drum 43 rotates in the circumferential direction, the four through holes 4 are always
Two projecting portions 59a, 5 which slidably contact the inner wall of the sampling drum 43 so as to trace the moving tracks of 5a to 45d.
9b are formed, and these protrusions 59a and 59b are formed so that when the two collecting pieces and the chambers 51 and 52 face each other, as shown in FIG. They are arranged at an angle of °. A second communication hole 61a is formed to communicate with the external pressurizing / depressurizing pump 60 from the opening of the tip of the protrusion 59a.
A third communication hole 61b that communicates with another external pressurizing / depressurizing pump (not shown) from the tip opening of b is formed.
Therefore, as shown in FIG. 7, the chambers 51, 52 and 2
When the individual collection pieces (for example, 44c and 44d face each other), the chamber 51 and the second communication hole 61a communicate with each other, and the chamber 52 and the third communication hole 61b communicate with each other.

Reference numeral 62 is a pressure sensor provided at one end of the first communication hole 58 for measuring the internal pressure, and 63 is a pressure sensor provided at one end of the second communication hole 61a for measuring the internal pressure. , And transmits respective measurement signals to a control unit (not shown). Although not shown, the third communication hole 61
A pressure sensor is also provided at b to transmit the measurement signal P3 to the control unit.

The control section is provided with arithmetic means such as a microprocessor, receives the measurement signal, and determines first, second, and third communication holes 58, 61a, 61b which are predetermined.
A control signal for setting the internal pressure of each pump 57,
60 and a pump (not shown). Furthermore, the control signal instructs the pressurizing / depressurizing pump 60 and a pressurizing / depressurizing pump (not shown) to perform a pressurizing operation and a depressurizing operation. The control unit also has a function of receiving a video signal from the video camera 53, performing image processing described later, and discriminating dust or abnormal cells from normal cells.

Next, the operation of this embodiment having such a configuration will be described. First, the decompression pump 57 is set to always perform decompression operation at a constant pressure. Further, by supplying a control signal of a predetermined timing from the control unit to the rotary drive motor 50, the sampling drum 43 is continuously rotated stepwise by 90 °, and the sampling pieces 44a to 44d are sequentially arranged in the petri dish portion 42. Soak in the solution.

The processing operation by the collecting piece 44a will be described as a representative example. When the collecting piece 44a is immersed in the solution in the Petri dish portion 42, the negative pressure in the chamber 56 causes the collecting piece 44a.
One cell is aspirated into the micropores of. Next, when the sampling drum rotates 90 °, the video camera 53 images the cells still adsorbed on the sampling piece 44a, and the video signal is transmitted to the control unit. The control unit compares the image data of a predetermined type of cell set in advance with the transmitted image signal by image processing, judges that it is a normal cell if it meets a predetermined criterion, and should collect it. To determine. On the other hand, those that do not meet the criteria are judged to be abnormal cells or dust and are judged to be discarded.

Next, when the sampling drum 43 is rotated by 90 °, the sampling piece 44a faces the chamber 51. At this switching time point, the pressurizing / depressurizing pump 60 and the pressurizing / depressurizing pump (not shown) are depressurized, and when the control unit determines that the cells are normal cells, the depressurizing operation is continued. Therefore, normal cells remain adsorbed on the collected piece 44a. On the other hand, when it is determined that the cells are abnormal cells or dust, the pressurizing / depressurizing pump 60 is switched to the pressurizing operation. Therefore, the second
As the pressure in the communication hole 61a of the cell rises, abnormal cells and the like are discharged to the discarding chamber 51 and are discarded.

Next, when the sampling drum 43 is further rotated by 90 °, the sampling piece 44a faces the sampling chamber 52,
In the case of normal cells, the pressurizing / depressurizing pump (not shown) is switched to the pressurizing operation for the first time at this opposing time point. As a result,
Normal cells are discharged into the chamber 52 and collected. Although the sampling operation for the sampling piece 44a has been described above, the pressurizing / depressurizing pump 60 and the pressurizing / depressurizing pump (not shown) for the other sampling pieces 44b to 44d also perform the pressurizing / depressurizing operation according to the rotation position of the sampling drum 43, and the sampling The imaged cells are imaged by the video camera 53, the quality of the cells, the dust, etc. are judged from the video signal, and only the cells satisfying a predetermined standard are conveyed to the collection chamber 52, and unnecessary substances are discarded. Discard into the chamber 51 for use.

[0039]

As described above, according to the present invention, minute holes each having a diameter of cells are provided on the side surface of the collecting drum having a cylindrical shape or a polygonal prism shape, and the inside of the collecting drum is outside the minute holes. A means for alternately applying negative pressure and positive pressure air is provided, and the sampling drum is continuously rotated with the side end of the sampling drum immersed in the liquid phase containing cells, and the micropores are in the liquid phase. The cells are sucked into the micropores by applying a negative pressure to the micropores by the above-mentioned means at the timing of dipping into the micropores, and the sucked cells are selected by performing image discrimination processing at the timing when the micropores rise from the liquid phase. When it is judged to be unsuitable by the selection, the micropores are removed from the micropores by applying a positive pressure to the micropores by the above-mentioned means, and when it is judged to be suitable, the micropores are applied to a positive pressure by the above-mentioned means to obtain a predetermined accommodation means. Transported to With this configuration, according to such a configuration, it is possible to continuously select predetermined cells even when processing a large number of types and a large number of cells. It is possible to automatically perform the processing of sorting, transportation.

[Brief description of drawings]

FIG. 1 is an explanatory diagram showing a configuration of an exemplary embodiment of the present invention.

FIG. 2 is a cross-sectional view showing a vertical cross-sectional area structure of the sampling drum or the like of FIG.

FIG. 3 is a perspective view showing the shape of a sampling piece.

FIG. 4 is an explanatory diagram showing a configuration of another embodiment of the present invention.

5 is a sectional view showing a vertical sectional structure of the sampling drum and the like of FIG.

FIG. 6 is an explanatory diagram showing a configuration of still another embodiment of the present invention.

7 is a sectional view showing a horizontal sectional structure of the sampling drum and the like in FIG.

FIG. 8 is an explanatory diagram illustrating a conventional cell manipulating means.

[Explanation of symbols]

3,21,42 Petri dish 6,24,43 Collection drum 7a-7d, 25a-25d, 44a-44d Collection piece 14a, 14c, 39,60 Pressurization pressure pump 36,57 Pressure reduction pump 12a, 12c, 37,38a, 38b. , 58, 61
a, 61b communication hole 15, 16, 31, 32, 51, 52 chamber 17, 33, 53 video camera

Claims (4)

[Claims] 1. A cylinder or a polygonal column having a single or a plurality of minute holes on its side surface, a rotating mechanism for rotating the column, and a suction and discharge pump attached to the column, wherein suction is performed from the minute holes. The isolated cells in the liquid phase are sucked together with the liquid into the micropores, and suction pressure is generated to adsorb the cells and hold them so that they do not fall off. A cell transport device that allows individual cells that have been isolated in the liquid phase to be transported by suction by ejecting the cells every night when they are inhaled. 2. A cylinder or a polygonal column having a single or a plurality of minute holes on its side surface, a rotation mechanism for rotating the column, and a suction and discharge pump attached to the column, and suction is performed from the minute hole. The isolated cells in the liquid phase are sucked together with the liquid into the micropores, a suction pressure is generated to adsorb the cells and hold them so as not to drop them, and the micropores are rotated to allow the camera to rotate. The micropore is photographed by a camera at a position where the micropore has been rotated to a position where it can be observed, and the desired cell is adsorbed or not desired by the pattern recognition of the image information. A cell transfer device that determines whether or not cells are adsorbed, discharges the desired cells and undesired cells together in different locations, and collects or transfers only the desired cells individually. 3. A column or polygonal column having a single or a plurality of micropores on its side surface and further having a cavity connected to the micropore therein, a rotating mechanism for rotating the column, and a suction provided outside the rotating mechanism. , A discharge pump, a discharge mechanism of the pump, a pressure transmission mechanism for transmitting suction pressure to keep the entire cavity at a negative pressure, and a nozzle having an opening in contact with or close to the inside of the rotating column to change suction force It is possible to detect that the isolated cells in the liquid phase are aspirated and adhered to the micropores by using the pressure control mechanism that holds the cells in the micropores by appropriate pressure control. A cell transport device that allows individual suctioned movement of separated cells. 4. A cylinder or a polygonal column having a single or a plurality of micropores on its side surface and further having a cavity connected to the micropore therein, a rotating mechanism for rotating the column, and a suction provided outside the rotating mechanism. , A discharge pump, a discharge mechanism of the pump, a pressure transmission mechanism for transmitting a suction pressure to keep the entire cavity at a negative pressure, and a nozzle having a port in contact with or close to the inside of the rotating column, thereby changing the suction force It is possible to detect that the isolated cells in the liquid phase are aspirated and adhered to the micropores by using the pressure control mechanism that holds the cells in the micropores by appropriate pressure control. The cells that have been separated can be sucked and moved individually, and the micropores are rotated by the camera to a position where the camera can observe the micropores. Request the image information by pattern recognition Judging whether or not cells are adsorbed by the micropores or adsorbed undesired ones, and ejecting desired cells and undesired ones to different places, only the desired cells are individually collected. Or a cell transfer device for transferring.