JPH0556945B2 - - Google Patents
Info
- Publication number
- JPH0556945B2 JPH0556945B2 JP488587A JP488587A JPH0556945B2 JP H0556945 B2 JPH0556945 B2 JP H0556945B2 JP 488587 A JP488587 A JP 488587A JP 488587 A JP488587 A JP 488587A JP H0556945 B2 JPH0556945 B2 JP H0556945B2
- Authority
- JP
- Japan
- Prior art keywords
- virus
- cells
- chuzan
- inoculum
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000700605 Viruses Species 0.000 claims description 21
- 241000283690 Bos taurus Species 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 230000002159 abnormal effect Effects 0.000 claims description 4
- 241000702259 Orbivirus Species 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 37
- 241001299893 Chuzan virus Species 0.000 description 19
- 239000002054 inoculum Substances 0.000 description 16
- 239000000243 solution Substances 0.000 description 13
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 12
- 239000002953 phosphate buffered saline Substances 0.000 description 12
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 210000000601 blood cell Anatomy 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 6
- 230000004663 cell proliferation Effects 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 5
- 238000007796 conventional method Methods 0.000 description 5
- 239000012888 bovine serum Substances 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000256135 Chironomus thummi Species 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 206010070834 Sensitisation Diseases 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000000120 cytopathologic effect Effects 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 230000008313 sensitization Effects 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- FXKZPKBFTQUJBA-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium;dihydrate Chemical compound O.O.[Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O FXKZPKBFTQUJBA-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241001222053 Akabane virus Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 241000120518 Palyam virus Species 0.000 description 1
- 241000702247 Reoviridae Species 0.000 description 1
- 241000251778 Squalus acanthias Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000005821 brain abnormality Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 208000005093 cerebellar hypoplasia Diseases 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 201000009075 hydranencephaly Diseases 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 208000002254 stillbirth Diseases 0.000 description 1
- 231100000537 stillbirth Toxicity 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP488587A JPS63173584A (ja) | 1987-01-14 | 1987-01-14 | 牛の流行性異常産ウィルス株の作出法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP488587A JPS63173584A (ja) | 1987-01-14 | 1987-01-14 | 牛の流行性異常産ウィルス株の作出法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63173584A JPS63173584A (ja) | 1988-07-18 |
| JPH0556945B2 true JPH0556945B2 (enrdf_load_stackoverflow) | 1993-08-20 |
Family
ID=11596132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP488587A Granted JPS63173584A (ja) | 1987-01-14 | 1987-01-14 | 牛の流行性異常産ウィルス株の作出法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63173584A (enrdf_load_stackoverflow) |
-
1987
- 1987-01-14 JP JP488587A patent/JPS63173584A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63173584A (ja) | 1988-07-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| EXPY | Cancellation because of completion of term |