JPH0556321B2 - - Google Patents

Description

[Detailed Description of the Invention] The present invention is used to preserve organs such as hearts or kidneys removed from humans or other animals until transplanted to other patients or animals, or to remove and treat organs and then use them. The present invention relates to an organ preservation method and preservation device for extracorporeal treatment to restore the original state.

Conventionally, organs extracted from the human body have been preserved by freezing or keeping them at a cold temperature.

However, with this method, the period during which organ tissue can be kept alive is extremely short;
For example, one drawback is that the tissue in the removed organ dies before it can be transplanted to the patient who needs it.

An object of the present invention is to eliminate such drawbacks and provide an organ preservation method and a preservation device that enable cell tissues of organs extracted from the body to be preserved alive for a long period of time. Regarding the preservation method of removed organs, the characteristics are that the organs removed from the body are stored in a sealed container with high pressure inside.
The second invention relates to a storage device for an extracted organ, which is characterized by storing an organ removed from the body and having a high pressure inside. It consists of a sealed container and a preservation solution supply device that supplies a preservation solution containing dissolved high-pressure oxygen to the artery of the organ. Next, one embodiment of the present invention will be described with reference to FIGS. 1 and 2.

1 indicates a sealed container, and the sealed container 1 is a tub body 1a.
and a lid 1b that is screwed together to seal the opening of the container 1a.Inside the sealed container 1, an organ 2 extracted from a human body is housed, and for example,
Kg/cm ² of high pressure oxygen 3 was sealed.

4 indicates a storage solution stored in the sealed container 1.
The preservation solution 4 supplies oxygen and nutrients necessary for the cells of the organ 2 instead of blood, and it is suitable to use Krebs' solution or physiological saline mixed with nutrients.

Reference numeral 5 denotes a preservation solution supply device that supplies the preservation solution 4 to the artery of the organ 2;
includes a liquid feeding tube 5a with one end communicating with the bottom of the sealed container 1 and the other end communicating with the cut end 2a of the artery of the organ 2, and a liquid feeding tube 5a provided outside the sealed container 1 in the middle of the liquid feeding tube 5a. A high-pressure oxygen cylinder 5d communicates with the upper part of the sealed container 1 via a communication pipe 5c, an on-off valve 5e provided in the middle of the communication pipe 5c, and a A preservation solution storage tank 5g that communicates with the high-pressure oxygen cylinder 5d and communicates with the sealed container 1 at the lower part via a liquid feeding pipe 5f, an on-off valve 5h provided in the middle of the liquid feeding pipe 5f, and the sealed container 1. It consists of a drain valve 5i provided at the bottom of the tank.

6 and 7 are PHs provided at the bottom of the sealed container 1.
Meter and liquid level detector, 8 indicates a pressure gauge provided at the upper part of the sealed container 1, and 9 indicates a control circuit that controls opening and closing of the on-off valves 5e, 5h and the drain valve 5i based on output signals from these.

Reference numerals 5j and 5k indicate opening/closing pots provided at the heads of the high-pressure oxygen cylinder 5d and the storage solution storage tank 5g, respectively. These opening/closing locks 5j, 5k are provided to close when replacing the liquid storage tank 5g to prevent leakage of high pressure oxygen or storage liquid.

Reference numeral 10 indicates an on-off valve provided on the lid 1b of the sealed container 1. When opening the lid 1b when the inside of the sealed container 1 is under high pressure, the on-off valve 10 is first opened to greatly expand the inside of the sealed container 1. It is provided to lower the atmospheric pressure.

Next, a method for preserving the extracted organ using the above device will be explained.

With the on-off valves 5e and 5h closed, the lid body 1b is opened and the extracted organ 2 is accommodated in the tub body 1a,
Next, after closing the lid 1b, the on-off valve 5h is opened, and the upper surface of the preservation solution 4 in the preservation solution storage tank 5g is pressed by high pressure oxygen from the high pressure oxygen cylinder 5d, and the preservation solution 4 is transferred to the liquid supply pipe 5f. A predetermined amount is delivered into the sealed container 1 through the . Note that the opening/closing handles 5j and 5k are in an open state. Thereafter, the on-off valve 5h is closed, and the on-off valve 5e is used to send high-pressure oxygen into the sealed container 1 to bring the inside of the sealed container 1 into a high-pressure oxygen state at a pressure of 3 kg/cm ² , and the on-off valve 5e is closed.

In this state, the storage solution 4 in the sealed container 1
is under the pressure of high pressure oxygen 3 of 3Kg/cm ² , so
According to Henry's law, approximately three times the mass of oxygen is dissolved compared to that at atmospheric pressure. and,
By operating the liquid pump 5b, the preservation solution 4 containing a large amount of oxygen is supplied to the artery of the organ 2, and the preservation solution 4 flows through the blood vessels in the organ 2 and is absorbed into the cell tissue of the organ 2. After supplying a sufficient amount of oxygen and nutrients to the artery, it flows out from the cut end of the artery and returns to the bottom of the sealed container 1.

By repeating this operation, the tissue of the organ 2 is always supplied with sufficient amounts of oxygen and nutrients, and can be preserved alive for a long period of time.

In addition, a storage solution containing 3 kg/cm ² of high pressure oxygen is used.
If it is supplied to the artery of organ 2 under atmospheric pressure, there is a risk that the blood vessel will expand and be damaged due to the pressure difference, but in this example, the surrounding area of organ 2 was also kept at a high pressure of 3 kg/cm ² , so it could flow inside and outside the blood vessel. There is no pressure difference and the tissue will not be damaged.

When the storage solution 4 in the sealed container 1 becomes contaminated, the storage solution 4 gradually becomes acidic. In this example,
This can be automatically detected, and the high pressure oxygen 3 and storage solution 4 in the sealed container 1 can be automatically replaced with fresh ones. The exchange is performed in the following steps.

(1) When an output signal indicating acidity is sent from the PH sensor 6 to the control circuit 9, the control circuit 9 issues an output signal to open the drain valve 5i, thereby opening the drain valve 5i.

After a certain period of time has elapsed, the storage liquid 4 and high pressure oxygen 3 in the sealed container 1 are discharged, and then the drain valve 5i is closed by an output signal from the control circuit 9.

(2) The liquid sending pipe 5 is controlled by the output signal from the control circuit 9.
Open the on-off valve 5h provided at f. Since the pressure of high pressure oxygen in the high pressure oxygen cylinder 5d is applied to the storage liquid storage tank 5g, the storage liquid in the storage liquid storage tank 5g is sent into the sealed container 1 by the pressure. Therefore, power such as a pump is not required.

When the preservation liquid reaches the position of the liquid level detector 7 in the sealed container 1, the control circuit 9 is activated by a signal from the liquid level detector 7, and the on-off valve 5h is closed.

(3) Next, in response to a signal from the control circuit 9, the on-off valve 5e provided in the communication pipe 5c is opened, and the high-pressure oxygen in the high-pressure oxygen cylinder 5d is sent into the sealed container 1. When the output signal from the pressure gauge 8 indicates 3 kg/cm ² , the control circuit 9 is activated and the on-off valve 5 is activated.
Close e to complete the exchange operation.

In the above embodiment, the exchange of high pressure oxygen and storage solution was performed automatically, but this may also be performed manually.

In this way, explanted organs can be preserved for an extremely long period of time by replacing the hyperbaric oxygen and preservation solution with fresh ones, but even if replacement is not done, organs cannot be stored until the contamination becomes significant. Since it can be stored, the above replacement is not necessarily necessary unless the storage period is extremely long.

Further, in the above embodiment, the high pressure oxygen 3 and the preservation solution 4 were placed in the sealed container 1, and the high pressure oxygen was dissolved in the preservation solution within the sealed container 1. However, the present invention is not limited to this, and for example, Even if high pressure oxygen is dissolved in a preservation solution outside the sealed container 1, the pressure inside the sealed container 1 is maintained at a high level to the extent that the vascular tissue of the organ 2 is not damaged by the pressure of the dissolved high pressure oxygen. good.

In addition, in the above example, the pressure of hyperbaric oxygen was 3 kg/cm ² , but it is not limited to this, and the optimal pressure can be selected depending on the type of organ or the amount of oxygen required for the organ. can do.

Further, in the above embodiment, the liquid feeding pipe 5a having the liquid feeding pump 5b was arranged outside the sealed container 1.
The liquid feeding pump 5b and the liquid feeding pipe 5a may be arranged inside the sealed container 1.

As described above, according to the first invention, since the preservation solution in which high-pressure oxygen is dissolved is supplied to the artery of the organ, a sufficient amount of oxygen and nutrients can always be supplied to the tissue of the excised organ. According to the second invention, a high-pressure sealed container and a preservation solution supply device that supplies the preservation solution to the artery of the organ are used. Since the organ preservation device is configured, the device is extremely small and has the advantage of being able to preserve organ tissue for a long period of time.

[Brief explanation of the drawing]

FIG. 1 is a sectional view of one embodiment of an apparatus for carrying out the present invention, and FIG. 2 is a block diagram of a control circuit for automatically replacing a storage solution, etc. 1... sealed container, 2... organ, 3... hyperbaric oxygen, 4...
Preservation liquid, 5...Preservation liquid supply device, 5a...Liquid sending pipe, 5
b...Liquid pump, 5d...High pressure oxygen cylinder, 5e...
On-off valve, 5g... Storage liquid storage tank, 5h... On-off valve, 5i... Drain valve.

Claims (1)

[Scope of Claims] 1. An organ extracted from the body is housed in a sealed container whose interior is kept under high pressure, and a preservation solution in which high pressure oxygen is dissolved is supplied to the artery of the organ. How to preserve organs. 2. Preservation of organs removed from the body, characterized by the use of a sealed container that houses organs removed from the body and has a high pressure inside, and a preservation solution supply device that supplies a preservation solution containing dissolved high-pressure oxygen to the artery of the organ. Device. 3. Claim 2, characterized in that the storage solution supply device comprises a conduit that communicates the bottom of the sealed container with the artery of the organ, and a liquid pump provided in the middle of the conduit. Preservation device for excised organs as described in Section 1. 4. The storage solution supply device includes a conduit that communicates the bottom of the sealed container with the artery of the organ, a liquid pump provided in the middle of the conduit, and a high-pressure pump that communicates with the sealed container via an on-off valve. An oxygen cylinder, a preservation solution storage tank whose upper part communicates with the high-pressure oxygen cylinder and whose lower part communicates with the sealed container via an on-off valve, and a drain means for discharging the preservation solution from the sealed container. A storage device for an extracted organ as claimed in claim 2.