JPH0555063U - Chromatographic equipment - Google Patents

Abstract

(57) [Summary] [Purpose] In supercritical fluid chromatographs and other analyzers that use fluid as the mobile phase, the fluid resistance of the mobile phase can be arbitrarily changed to freely change the flow rate or pressure of the mobile phase due to pressure fluctuations. To provide a chromatograph device capable of performing. [Structure] The pressure medium 3 is contained in a container 2 in which the pressure medium 3 is enclosed.
A pressurizer 5 for pressurizing the pressure is connected, and a flexible tube 6 for circulating a mobile phase is passed through the container 2 so as to be in contact with a pressure medium. −
A chromatograph device characterized in that the diameter of the stud 6 is changed.

Description

[Detailed description of the device]

[0001]

[Industrial application]

 The present invention relates to a chromatographic device capable of controlling the flow rate and pressure by changing the fluid resistance of a mobile phase in a supercritical fluid chromatograph, for example.

[0002]

[Prior Art]

 As shown in FIG. 4, the supercritical fluid chromatograph is a gas chromatograph type (hereinafter, referred to as GC type) in which a mobile phase is discharged from a syringe pump 11 through a column 12, a restrictor 10 and a gas chromatograph detector 13. As shown in FIG. 5, there is a liquid chromatograph type (hereinafter, referred to as LC type) in which a plunger pump 14, a column 15, a liquid chromatograph detector 16 are passed and a mobile phase is passed and discharged. The GC type is a combination of a syringe pump and a fixed resistance restrictor (resistor) that uses a capillary tube, and the LC type is a combination of a plunger pump and a back pressure valve. The GC type controls the pressure, and the LC type controls the temperature by keeping the flow rate and back pressure constant, or uses two plunger pumps to use a modifier gradient (mixing ratio of liquid with different polarity from mobile phase). And then add). Usually, a flame ionization detector (FID) is most often used as the GC type detector 13, and an ultraviolet spectrophotometer (UVD) is most often used as the LC type detector 16.

[0003]

[Problems to be solved by the device]

 When a sample is analyzed by a supercritical fluid chromatograph, the GC type changes the pressure of the syringe pump but does not control the flow rate, which may affect the reproducibility of the analytical value. Although the LC type can keep the flow rate constant, it cannot use the back pressure valve to set the mobile phase pressure to any pressure. Further, if a back pressure valve is used in the LC type, a hydrogen flame ionization detector (FID) cannot be used, which is disadvantageous in terms of sensitivity. The present invention has been made in view of such a problem, and its purpose is to change the fluid resistance of the mobile phase arbitrarily in an analyzer using a fluid as the mobile phase, such as a supercritical fluid chromatograph, to accompany changes in pressure. An object of the present invention is to provide a chromatograph device capable of freely setting the flow rate change or the mobile phase pressure.

[0004]

[Means for Solving the Problems]

 That is, according to this invention, in order to solve the above-mentioned problems, a chromatograph apparatus connects a container in which a liquid serving as a pressure medium is sealed with a pressurizer for pressurizing the pressure medium, and further moves to the container. It is characterized in that a flexible tube for circulating the phase is passed through in contact with the pressure medium, and the diameter of the flexible tube is changed by the pressurizer.

[0005]

[Action]

The operation when the chromatographic apparatus according to the present invention is used as the above means will be described with reference to the attached drawings (FIGS. 1 to 3). When the chromatographic apparatus 1 comprising the above means is applied to a gas chromatograph type supercritical chromatograph (Fig. 2), the syringe pump 11 changes the pressure upstream of the column 12 in accordance with the pressure program. The tube 6 of the device 1 can be pressurized by the pressurizer 5 and the pressure medium 3 to change the fluid resistance of the mobile phase so that the flow rate of the mobile phase flowing through the column 12 is always constant. Next, when the chromatograph apparatus 1 including the above means is applied to a liquid chromatograph type (FIG. 3), a pressure gauge 18 is arranged upstream of the chromatograph apparatus 1 and the numerical value P _{G of the} pressure gauge 18 is set. The tube 6 of the chromatographic apparatus 1 is pressurized by the pressure medium 3 so that the pressure becomes a desired pressure, and the fluid resistance of the mobile phase is changed to perform supercritical analysis. At this time, since the plunger pump 14 produces a constant flow rate, the pressure in the column 15 can be arbitrarily set by changing the flow rate resistance of the mobile phase by the chromatograph device 1. Of course, the chromatographic apparatus 1 can also be connected to the GC detector 13 if necessary.

[0006]

【Example】

 Hereinafter, a specific embodiment of the present invention will be described with reference to the drawings. FIG. 1 is a sectional view showing the structure of a chromatographic apparatus 1 according to the present invention. In this device, a hole 2a is formed in a container 2 in which a pressure medium 3, for example, a liquid such as water, oil or alcohol is sealed, and a pipe 4 to which a pressurizer 5 is connected is fitted into the hole 2a. It is embedded. As the pressurizer 5, a syringe capable of adjusting the hydrostatic pressure, various pumps, or the like can be used.

Next, a flexible tube 6 for circulating a mobile phase is passed through the pressure medium 3 (or in contact with the pressure medium 3) through the container 2. The tube 6 is connected to a detector (not shown). The tube 6 is fixed to the container 2 with a joint 7. A pipe 8 for passing a mobile phase is connected to the upstream side of the joint 7, but the pipe 8 does not need to be flexible. That is, only the tube 6 arranged in the container 2 has flexibility. In this way, when the pressurizer 5 is operated and the pressure of the pressure medium 3 is changed, the diameter of the flexible tube 6 is changed and the fluid resistance of the mobile phase flowing in the tube 6 is changed. Can be done.

The chromatographic apparatus 1 according to the present invention has the above-described configuration. Next, a specific example of use will be described. FIG. 2 shows a case where the chromatographic apparatus having the above-mentioned configuration is applied to the GC type. The syringe pump 11 changes the pressure upstream of the column 12 in accordance with the pressure program. In response to this, the tube 6 of the chromatographic apparatus 1 is pressurized by the pressurizer 5 and the pressure medium 3 and the fluid resistance of the mobile phase is increased. Can be changed so that the flow rate of the mobile phase flowing through the column 12 is always constant. That is, the resistance of the chromatograph device 1 can be controlled in accordance with the pressure program.

Next, FIG. 3 shows a case where the chromatograph apparatus 1 having the above-mentioned configuration is applied to an LC type. A pressure gauge 18 is arranged upstream of the chromatograph apparatus 1, and the tube 6 of the chromatograph apparatus 1 is moved by the pressure medium 3 so that the numerical value P _G of the pressure gauge 18 becomes a desired pressure. Supercritical analysis is performed by applying pressure to change the fluid resistance of the mobile phase. At this time, since the plunger pump 14 produces a constant flow rate, the pressure in the column 15 can be arbitrarily set by changing the flow rate resistance of the mobile phase by the chromatographic apparatus 1. The resistance of the chromatographic apparatus 1 is set so that feedback is applied from the pressure P _G displayed by the pressure gauge 18. Of course, the chromatograph device 1 can also be connected to the GC detector 13 if necessary.

[0010]

[Effect of the device]

 Since the chromatographic device according to the present invention has the above-described configuration, it can be used as a device capable of controlling the fluid resistance in either the GC type or the LC type. If the chromatographic device according to the present invention is installed in the GC type mobile phase distribution path, the change in the flow rate due to the pressure fluctuation can be suppressed, so that better reproducibility can be obtained, and the LC type path can be installed. If so, the pressure on the upstream side can be set freely, so a pressure program can be applied with a constant flow rate. Furthermore, it is easy to connect to the GC type detector, and there is no fear of precipitation of components inside the valve like a back pressure valve. can get.

[Brief description of drawings]

FIG. 1 is a cross-sectional view showing a configuration of a chromatographic apparatus according to the present invention.

FIG. 2 is a diagram showing a case where the chromatographic apparatus according to the present invention is applied to a GC type.

FIG. 3 is a diagram showing a case where the chromatographic apparatus according to the present invention is applied to an LC type.

FIG. 4 is a simplified diagram showing a configuration of a conventional supercritical fluid chromatograph, which is a diagram showing a gas chromatograph type.

FIG. 5 is a simplified diagram showing a configuration of a conventional supercritical fluid chromatograph, which is a liquid chromatograph type.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Chromatographic apparatus 2 Container 3 Pressure medium 4 Tube 5 Pressurizer 6 Flexible tube 7 Joint 11 Syringe pump 12 Column 13 Gas chromatograph detector 14 Plunger pump 16 Liquid chromatograph detector 18 Pressure gauge

Claims (1)

[Scope of utility model registration request] 1. A container enclosing a pressure medium is connected to a pressurizer for pressurizing the pressure medium, and a flexible tube for circulating a mobile phase is brought into contact with the pressure medium in the container. A chromatographic apparatus, characterized in that the diameter of the flexible tube is changed by the pressurizing device.